Biochemistry Lab

Specimen Receiving

Mr. MOHAMED A. ADAMMedical Lab Technologist

KCCC

LABORATORY WORK FLOW

Samples Received with

requests

Centrifugation of samples

Data are entered in the LIS to get

barcodeBarcodes are

stick on the tubes then distributed

to machine

Sample Processing

Results are Reported and

Released

Clean your

bench with fresh actichlor

WHAT WE ARE RECEIVE?

•Specimen type

• Whole Blood

1. Plasma

2. Serum

• Body Fluid

1. Cerebro-spinal Fluid CSF

Cerebrospinal fluid (CSF) is a fluid that surrounds the brain

and spinal cord.

CSF acts as a cushion, protecting the brain and spine from

injury. The fluid is normally clear same as water.

WHAT IS BLOOD

Phlebotomist draws sample

1.Gentlly invert five timesTo mix dot activator with blood

2. Allow blood to clot in a vertical position

Observe a dense clot

3.Centrifuge for 10 min. at 3000 rpm

SEPARATION OF SAMPLES (SERUM)

PRE-ANALYTICAL3000 rpm

10 minShould be balanced

WHAT IS THE DIFFERENCE BETWEEN SERUM AND PLASMA

VACUTAINER BLOOD COLLECTION TUBESRange Color coding Additive Clinical Use for

Whole bloodEDTA

Hematology testSpecial biochemistry (HbA1c)

Immunochemistry (Cyclosporin and Tacrolimus)

PlasmaLithium Heparin Cardiac Markers

Sodium Fluoride + Potassium Oxalate Glucose Test (FBS free blood sugar)

Sodium Citrate Coagulation test

SerumPlain (NA additive)

Clot activator

Serum biochemistry and immunology testTumor Markers

HormonesVitamin D (should be covered)

Oncology RFT – LFT – Lipid profileGel + Clot activator

SAMPLE TRANSPORT AND STORAGE

• Transport must be save

Biohazardous material

• 4 HOURS Stable at 15-25 ˚c

Close to avoid evaporation

• Stable at 2-8 ˚c

Dry Ice – Cool packs – refrigerator .. etc

24 hours only (for some biochemistry analyte such as K, )

LIST OF TESTS IN OUR LABTUMOR MARKERS

Test Diagnosis

CEA (No Spicificity) Colorectal, gastrointestinal,

lung and breast cancers

CA-15.3 Breast Cancer

CA-125 Ovarian Cancer

CA-19.9 Colorectal and Pancreatic

cancer

B2Microglobulin Blood cell cancer

Test Diagnosis

PSA

Total and Free

Prostate Cancer

AFP Liver Cancer

Cyfra 21-1 Lung Cancer

TG Thyroid Cancer

ATG

B-HCG Chorion Cancer

“Pregnant test”

LIST OF TESTS IN OUR LABBIOCHEMISTRY

Biochemistry Profiles

Oncology (biochemistry _ Full Profile)

Liver Electrolyte

Lipid Amylase

Renal Iron

HbA1c

Cardiac MarkersCK – CKMB - Troponin

Thyroid Function Test (TFT)TSH – FT4

Other Tests

Vitamin D

PCT

Cyclosporin

Tacrolimus

EACH SPECIMEN SHOULD BE LABELED WITH PATIENT`S INFORMATION

• Full Name• Age• Gender• File number or Civil ID• Doctor`s Stamp and signature• Clear and Specific test should be written• Cashier Stamp (If needed) for Vitamin D , Hormones and TFT

The personal information such (Name, file # or civil ID) MUST MATCH the same on the request