Crescentic GN

• This pattern of injury can be seen in three groups of diseases:

• 1. Anti-glomerular basement membrane (anti-GBM) disease, mediated by circulating anti-GBM antibodies; if associated with pulmonary capillaritis, it is reffered to as Goodpasture's syndrome.

• 2. Pauci-immune GN, most commonly triggered or mediated by anti-neutrophil cytoplasm antibodies (ANCA). Renal-limited forms are usually associated with p-ANCA. May present as pulmonary-renal syndrome (Wegener's granulomatosis), which is usually associated with c-ANCA.

• 3. Immune complex-mediated crescentic glomerulonephritides are usually associated with infections (postinfectious GN) or systemic diseases (lupus nephritis, class IV). Aggressive forms of IgA nephropathy/HSP-syndrome can also present with this form of glomerular injury.

Pauci-immune GN• Histopathology: • Early lesion is focal fibrinoid necrosis within the tuft; rupture of the GBM

causes plasma spillage in the Bowman’s space, with accumulation of fibrin and capillary thrombosis

• As the disease progresses, crescents occur, with involvement of more than 50% of glomeruli in most cases

• Not uncommon to see crescents of various stages (cellular, fibrocellular, or fibrous) at the same time

• Uninvolved segments and tufts appear unremarkable• Can be superimposed on other glomerular diseases, such as IgA nephropathy,

lupus nephritis, and others

• Immunofluorescence: • There is no significant deposition of immunoglobulins or complement. Fibrin

deposition may be demonstrated in injured segments of glomerular tufts and within the crescents.

• Electron microscopy: • Visceral epithelial cells: In the glomeruli with crescents, there

are numerous cells within the Bowman’s space, mostly epithelial cells and macrophages, with extracellular fibrin and sometimes necrotic debris. Visceral epithelial cells show various degrees of damage and effacement of foot processes

• Glomerular basement membranes: Segmental irregularities and wrinkling, with areas of disrupted continuity. Electron-dense deposits are typically not present, however, their presence does not exclude a concomitant ANCA-associated disease

• Glomerular endothelial cells: Usually show non-specific degenerative changes and do not contain tubuloreticular structures

• Mesangium: Normal cell elements and an extracellular matrix without electron-dense deposits

AntiGBM• Histopathology: • Early lesion is focal fibrinoid necrosis within the tuft; rupture of the GBM

causes plasma spillage in Bowman’s space, with accumulation of fibrin and capillary thrombosis

• As the disease progresses, glomerular extracapillary proliferation (crescents) occurs, with involvement of more than 50% of glomeruli in most cases

• All crescents in a given case are of the same age (e.g., cellular in acute or fibrocellular/fibrous in subacute/chronic phases)

• Uninvolved segments and tufts appear unremarkable (unless the anti-GBM disease is superimposed on some other glomerular disorder or disease)

• Immunofluorescence: • There is strong linear reactivity for IgG along the glomerular basement

membranes. Fibrin deposition may be demonstrated in injured segments of glomerular tufts and within the crescents

• Electron microscopy: • Visceral epithelial cells: In the glomeruli with crescents, the

Bowman’s space is packed with parietal epithelial cells, macrophages, extracellular fibrin, and sometimes necrotic debris. Visceral epithelial cells show various degrees of damage and effacement of foot processes

• Glomerular basement membranes: Segmental irregularities and wrinkling, with areas of disrupted continuity. Electron-dense deposits are typically not present in anti-GBM disease; however, some immune complex-mediated diseases may occur simultaneously, or rarely preceding or following the anti-GBM disease (e.g., membranous nephropathy{1})

• Glomerular endothelial cells: Usually show non-specific degenerative changes and do not contain tubuloreticular structures

• Mesangium: Normal cell elements and an extracellular matrix without electron-dense deposits

Lupus Nephritis Class IV• Histopathology: • Light microscopic examination reveals segmental or global

endocapillary proliferative changes. The mesangium is variably expanded and hypercellular

• The peripheral capillary loops are irregular in thickness, sometimes showing 'wire loops' and intraluminal 'microthrombi' (hyaline thrombi)

• Leukocyte infiltration, focal necrosis, hematoxilin bodies, and cellular crescents can all be seen

• In some cases, membranoproliferative pattern of injury may be dominant in glomeruli (class IV)

• The tubulointerstitium may show active interstitial nephritis

• Immunofluorescence: • There is 'full house' reactivity (reactivity for IgG, IgM, and IgA), with

granular deposits in the mesangium.

• Electron microscopy: • Visceral epithelial cells: Show different degrees of injury and

degenerative changes, with focal, but sometimes extensive, effacement of foot processes. Subepithelial deposits can be seen in many cases

• Glomerular basement membranes: May be irregular in thickness, with the presence of intramembranous, subepithelial, and/or subendothelial deposits. Subendothelial deposits can be rather large and may demonstrate substructural organization ('fingerprint'-like pattern)

• Glomerular endothelial cells: May contain tubuloreticular structures

• Mesangium: Expanded by increase in cellular elements and extracellular matrix, with sometimes large and confluent fine granular, electron-dense deposits

IgA Nephropathy• Histopathology: • Light microscopic examination reveals mesangial hypercellularity and

expansion of mesangial matrix, with segmental or global endocapillary proliferation and/or crescent formation

• If the number of involved glomeruli is below 50 percent, it is designated as focal proliferative pattern; if there are 50 percent or more glomeruli involved, the process is designated as diffuse proliferative IgA glomerulonephritis

• The tubulointerstitium may be unremarkable or show active inflammation or patchy fibrosis

• Immunofluorescence: • There is dominant reactivity for IgA in the mesangium; C3 may be

equally or less reactive. There is usually stronger reactivity for lambda than for kappa light chains in the mesangial deposits

• Electron microscopy: • Visceral epithelial cells: Show different degrees of injury and

degenerative changes; the effacement of foot processes is usually focal but sometimes extensive. Subepithelial and/or subendothelial deposits can also be present

• Glomerular basement membranes: May be thin; there is a higher incidence of thin glomerular basement membrane disease in IgA nephropathy than in any other glomerular disease {6}

• Glomerular endothelial cells: May show non-specific signs of injury; tubuloreticular structures are not seen

• Mesangium: Shows increase in cellularity and extracellular matrix, with fine granular electron-dense deposits that are sometimes large and confluent

Post Infectious GN• Histopathology: • Acute proliferative glomerulonephritis with numerous neutrophils and

endocapillary hypercellularity in all or most glomeruli• Cellular crescents are frequently seen in isolated glomeruli; true crescentic

form (involvement of 50% or more of glomeruli with crescents) is very unusual.• Fibrinoid necrosis and thrombosis are uncommon.• The tubulointerstitium may be unremarkable or show active inflammation

with or without edema.

• Immunofluorescence: • In acute phase, there is strong coarse granular C3 reactivity, with usually less

intense immunoglobulin (most commonly IgG) deposition. Lack of significant immunoglobulin reactivity may be seen in many cases. Three patterns of immunofluorescence reactivity have been described: starry sky (discrete random granules), garland (confluent subepithelial granular and band-like deposits), and mesangial pattern (usually during resolving phase).

• Electron microscopy: • Visceral epithelial cells: Different degrees of injury and

degenerative changes; the effacement of foot processes is usually focal, but sometimes extensive

• Glomerular basement membranes: May show irregularities in thickness. Subepithelial “hump”-like deposits are characteristic of postinfectious GN; they may be sometimes large and confluent. “Spike” formation is not characteristic of this entity. Sometimes large subendothelial deposits and an intraluminal increase in inflammatory cells may be seen as well

• Glomerular endothelial cells: May show non-specific signs of injury and reactive changes; tubuloreticular structures are not seen

• Mesangium: Increase in cellularity and extracellular matrix, with sometimes large and confluent fine granular electron-dense deposits