INSTRUMENTATION

QUOTE FOR THE DAY

OBJECTIVES• AT THE END OF THE LECTURE, YOU

SHOULD BE ABLE TO:

• 1. EXPLAIN THE PRINCIPLES OF THE METHODS.

• 2. DISCUSS THE FACTORS THAT MAY AFFECT THE METHODS.

• 3. KNOW THE IMPORTANCE OF THESE METHODS TO THE PROFESSION.

COLORIMETRY• VISUAL

• PHOTOELECTRIC

SPECTRONIC 20 D

CELL WELL

ON/OFCONTROL

0 ABSORBANCE CONTROL

ABSORBANCE /TRANSMITTANCE

CONTROL

DISPLAY WINDOWLED DISPLAY

MODEBUTTONSINCREASEDECREASE

CONCENTRATIONBUTTON

WAVELENGTHCONTROL

BEER LAMBERT’S LAW

SPECTROPHOTOMETRY

• BEER LAMBERT’S LAW O.D

Cu

% T

O.D.Cu

% T

Linear Graphing Paper

WHICH ONE IS MORE CONCENTRATED, BASED ON COLOR?

INTENSITY OF COLOR

v-frequency

wl

WAVELENGTHS

Infra red

visible

ultraviolet

Schematic of a single beam spectrophotometer

ELECTROPHORESIS

Gel Electrophoresis

PAPER CHROMATOGRAPHY

Completed Paper Chromatography of a Plant Pigment

ELECTROCHEMISTRY

TURBIDIMETRY

EMISSION FLAME PHOTOMETRY

EFP

ASPIRATOR

ATOMIZER

FLAME

VOLUMETRIC & TITRIMETRIC

GRAVIMETRIC

NEPHELOMETRY

FLUOROMETRY

ATOMIC ABSORPTION SPECTROSCOPY

AAS

AAS

AAS

MASS SPECTROPHOTOMETRY

OSMOMETRY

CHEMILUMINESCENCE

BRING OUT ¼ SHEET OF YELLOW PAPER

POST QUIZ• 1. Method that measures blocked light• 2. Method that applies the separation of

substances using mobile and stationary phase.• 3. Give the relationship of size to electrophoretic

mobility.• 4. In chromatography, what is the stationary

phase?• 5. Give one method that applies the separation of

ions in an electric field.• 6. Range of IR light.• 7. Range of visible light.• 8.-10 Name factors that affect EFP.• 11-15.Trascribe IEC,PC,GC,RBS, FBS

1. Method that measures Absorbed light2. Method that applies the separation of charged particles in an electric field.3. Give the relationship of density to electrophoretic mobility.4. In chromatography, what is the mobile phase?5. Give one method that separates ions/atoms from one another.6. Range of visible light.7. Range of uv light.8.-10 Name factors that affect electrophoresis method