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Oral Biofilm
ORAL BIOFILM
Oral Biofilm
A Biofilm is a community of microorganisms that adheres to substratum within a self-produced polysaccharide matrix and that show an altered phenotype compared to planktonic bacteria.
ORAL BIOFILM
Oral Biofilm
Microcolony 15-20% + Matrix 85-80%(water, bacterial cells,
proteins, exopolysaccharides , ….)
Essential for their functions
Oral Biofilm
The complexity of the bacteria SURVIVAL STRATEGY
Oral Biofilm
BIOFILM CONSEQUENCES?
Oral Biofilm
BIOFILM AND PERIODONTAL DISEASE
Polycaries
Oral Biofilm
BIOFILM MONOGRAPH
Oral Biofilm
New requirements in the therapeutic control of PD
The bacteria embedded in a biofilm are up to 1,000 times as resistant
Oral Biofilm
ANTISEPTIC MOUTHRINSES (affect the biofilm)
CHXCPC
TriclosanEssential oils
Hexetidine
IN VITRO TEST
(Artificial Mouth)
IN VIVO STUDIES
Oral Biofilm
In vitro studies (artificial mouth) CHLORHEXIDINE (CHX) greater bactericidal effect * but NOT all commercial brands showed the same efficacy (Herrera et al. 2003)
In vivo studies CHX mouthrinses able to penetrate the Biofilm and exert a greater bactericidal effect**. Mouthrinses with triclosan have little capacity to reduce the vitality of a biofilm (Arweiler)The effect of the CHLORHEXIDINE mouthrinses depended on their formulation. (Arweiler)
*In 2002 Shapiro compared the bactericidal capacity of CHX, Triclosan, Plant extracts, essential oils and hexetidine vs. destilled water in an artificial mouth **Netuschil
Oral Biofilm
Both in vitro and in vivo studies prove that combinations of CHX+CPC greater effect (Van Strydonck 2005)
Currently, lower levels of CHX (0.05%) are being used together with CPC, which improves its effect while reducing CHX’s side effects (Buscher &cols 2008)
Oral Biofilm
ADVANTAGES against planktonic bacteria
CHX ANTISEPTIC MOUTHRINSES
There are MORE AND MORE DIFFERENCES between CHX all the time
Oral Biofilm
Superior control over oral biofilm,for a better handle on gum disease.
Oral Biofilm
THE ONLY ONE THAT ACHIEVES MAXIMUM CONTROL OVER ORAL MICROBIAL LOAD
Oral Biofilm
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