- 1.Textile effluent biodegradation potentials oftextileeffluent-adapted and non-adapted bacteria Olukanni O. D.*, Osuntoki, A. A. and Gbenle, G. O.Presented by FAISAL
2. INTRODUCTION Industrialization is vital to anations economy There are associated problemsresulting from the introduction ofindustrial waste products into theenvironment. 3. The textile industries produce effluents thatcontains Dispersants Leveling agents Acids Alkalis carriers and various dyes (Cooper,1995.) 4. INTRODUCTION In many Nigerian cities,the textile factories dailydischarge millions oflitres of untreatedeffluents in the forms ofwastewater. (Olayinka and Alo,2004) This alters the pH, biochemical oxygendemand (BOD) chemical oxygendemand (COD), (Ajayi andOsibanjo, 1980). 5. Several methods are used in the treatment oftextile effluents filtration, specific coagulation, use of activated carbon and chemical flocculation. Some of these methods are effective but quiteexpensive(Do et al., 2002; Maier et al., 2004). 6. Biotreatment offers a cheaper andenvironmentally friendlier alternative for colorremoval in textile effluents. The ubiquitous nature of bacteria makesthem invaluable tools in effluentbiotreatment. 7. The chemical nature of dyes varies, but azodyes are the most widely used. The decolourization of azo dyes has beenfound to be effective under anaerobicconditions. The anaerobic degradation yields aromaticamines which are mutagenic and toxic tohumans and cannot be metabolized furtherunder the conditions which generated them(Chung and Stevens, 1993; Do et al.,2002). 8. To exploit the biodegradation abilities of ourindigenous microbial flora for remediativepurposes. The study is aimed to discovering isolateswith the potential for use in biologicaltreatment of textile effluents. 9. MATERIALS ANDMETHODS 10. Sources of organism Textile effluent-adapted bacteria were isolatedfrom effluent samples collected from thedischarge and drainage pipes of three textileindustries. The textile effluent non-adapted bacteria wereisolated from soil. Isolations were done on nutrient agar usingenrichment culture techniques and theorganisms identified to the generic level 11. A stock dye solution is prepared by dissolving5.6 g dyes mix/L. 12. In 20 ml simulated effluent, 2 x 10 cfu of the 5isolate was added in transparent bottles After 14 days decolourization and CODremoval were measured Decolourization was determined bymeasuring the absorbance of the simulatedeffluent on uv spectrophotometer. 13. The isolates were screened for plasmidsusing the plasmid isolation technique (kadoand lin, 1981)by electrophoresis on 0.8%agarose gels. The gels were stained withethidium bromide and viewed on a UVtransilluminator. 14. RESULTRESULTS 15. Source and identity ofisolates A total of 24 isolateswere obtained. eighteen organismsisolated from the textileeffluents Bacillus, Acinetobacter, Legionella, Staphylococcus and Pseudomonas 16. six isolates from the soil sample. Bacillus spp. 17. The majority of the effluent adapted isolatedshowed colour-removing activities between40.74 and 47.73% where as non-adaptedisolates show 40.25 to 46.63. while the COD activities are between 17.91and 36.69% of adapted isolates and nonadapted show high COD. 18. Plasmids were not detected in any of theisolates from the effluent adapted or non-adapted isolates. 19. DISCUSSION 20. In textile industries, During the dyeingprocess a substantial amount of dyes andother chemicals are lost in the waste water. Dye losses at between 1015% (Vaidya and Datye,1982). In the environment, dyes color water bodiesand may hinder light penetration therebyaffecting aquatic life and limiting theutilization (Ajayi and Osibanjo, 1980; Goncalves et al., 2000). 21. It has been reported that a typical textileeffluent contains a dye mass concentration of1050 mg/L (Clarke and Anliker, 1980).However, the human eye can detect levels aslow as 0.005 mg/L of reactive dyes in a clearriver (Pierce, 1994). 22. In our study a sample with a dye mass of 5.6g/L was used. This study discovered effluent adaptedstrains of Acinetobacter, Bacillus and Legionella with potentials forcolor removal and Strains of Acinetobacter, Bacillus andPseudomonas with potential use for CODremoval 23. very few strains can withstand the conditions ofdyeing effluents(Maeir et al., 2004) 24. we have discovered textile effluent adaptedstrains of Acinetobacter and Bacillus, andeffluent non-adapted Bacillus species withpotential use in effluent treatment. 25. Questions ?