1. Product InformationInteractive PDF internet-Link video/Animation Release 1.0Axio Vert.A1 Simply Get All Information from Your Cells

2. All Contrasts. All Information. All Flexible. Axio Vert.A1 Simply Get All Information from Your Cells In Brief The AdvantagesChoose from all standard contrasting techniques, including DIC, to investigate your cell cultures. Axio Vert.A1 produces brilliant images to answer your questions. Axio Vert.A1 is the only system in its class with such a large range of features, compact enough in fact to sit directly beside your incubator. Look into the very essence of your research while keeping your cell culture in its own protected environment. The Applications Animation The System Technology and Details ServiceAnimation2 3. Axio Vert.A1: Simpler. More Intelligent. More Integrated. Axio Vert.A1Using All Standard Contrast Techniques,LED Excitation Simply Get All InformationIts in a Class of its OwnGentle on Your Living Samplesfrom Your CellsBightfield, Phase contrast, PlasDIC, VAREL,You are working with fluorescence-labeled cells orEase of use is built right into Axio Vert.A1:improved Hoffman Modulation Contrast (iHMC)specify transfection rates? With Axio Vert.A1 yourWhether you are sitting or standing, simply useand Fluorescence contrast AxioVert.A1s rangesamples remain safe in gentle LED light.intermediate pieces to work comfortably in anreads like an index of contrast techniques. AloneThis microscope brings you tomorrows standardupright position. The ergo tube lets you tilt thein its class, it also employs Differential Interferencetoday:eyepieces as much as you need. You can alwaysContrast. With DIC you visualize even the finest LED excitation has no unwanted UV component,view your samples easily. Thanks to ergonomic In Brief The AdvantagesErgonomic Design Makes Your Work Easierstructures in your cells. And the new IVF contrast The Applicationsso you will see a significant increase in thedesign of all operating parts, your routine taskssystem is particularly impressive in IVF labs: withoutsurvival rate of your cells.and workflows will be easier especially whenmodifying the stand, you can switch freely between The SystemiHMC, PlasDIC and DIC as you investigate your samples. Technology and Details Profit from an extremely long life time of theyoure in a particular hurry.light source. Homogeneous illumination saves you time and bother on adjustments. LED illumination immediately works with full Serviceintensity there is no heating and cooling period required.3 4. Your Insight Into the Technology Behind it Axio Vert.A1The IVF contrast system unites iHMC, PlasDIC and DIC without modificationSimply Get All InformationThe new IVF contrast system is particularly effective in IVF labs: switch at will between iHMC, PlasDICfrom Your Cellsand DIC without modifying the stand. Axio Vert.A1 unites all three contrast techniques in one condenser and gives you enough room for micromanipulation. In BriefThe stable transmitted light arm provides precision you can rely on Adapt all standard micromanipulators to the stable transmitted light arm of your Axio Vert.A1. Your work The Advantageswill be free of vibration: you regulate your movements under the microscope with extreme precision. The Applications The System iHMC reveals even finest structures ServicePlasDIC improves your ICSI resultsDIC delivers brilliant images for IMSIYou examine embryos and assess cell Technology and DetailsStructures such as the Zona pellucida of oocytes areTop marks to DIC with IMSI for producing brilliantnuclei? iHMC shows nucleus shape and nucleolibrought out particularly well by PlasDIC contrastimages at high magnifications. You can easilybrilliantly.technique. You can judge precisely where to inject,assess the shape and vacuole count of sperm cells.in the cell nucleusthanks to the strong and brilliant relief effect.Embryo: Nucleus with nucleoli visible in right cellICSI: Oocyte with Zona pellucidaIMSI: Vacuoles in sperm cellsiHMCPlasDICDIC4 5. Axio Vert.A1 at Work Axio Vert.A1 Simply Get All Information from Your Cells In Brief The Advantages The ApplicationsKeratinocytis of epidermisHeLa cellsEmbryo: nucleus with nucleoli visible in right cellDICPlasDICiHMCTobacco, protoplastsHeLa cellsBrightfield2-channel fluorescence The System Technology and Details ServiceHeLa cells Phase contrastAnimation5 6. Axio Vert.A1: Your Flexible Choice of Components. Axio Vert.A1 Simply Get All Information from Your Cells 5 In Brief 4 The Advantages23 The Applications The System1 Technology and Details Service 1 Microscope3 Illumination4 Cameras Axio Vert.A1, transmitted light Transmitted light: halogen lamp, LED (wave-Recommended cameras: Axio Vert.A1 FL, transmitted light and fluorescence Axio Vert.A1 FL-LED,length 400 to 700 nm, peak at 460 nm) Reflected light: HBO 50, HBO 100, HXP 120 C, AxioCam ERc 5s AxioCam ICc 12 Objectives AxioCam ICc 3445, 455, 470, 505, 530, 590, 615, 625 or AxioCam ICm 1neutral white: 540 - 580 nmtransmitted light and LED fluorescenceLED modules (wavelength, nm): 365, 385, 420, AxioCam MRc AxioCam MRm A-Plan LD A-Plan5 Software LD Plan-NEOFLUAR ZEN lite ZEN pro6 7. Axio Vert.A1: System Overview Axio Vert.A1 Simply Get All Information from Your Cells In Brief The Advantages The Applications The System Technology and Details Service7 8. Technical Specifications Axio Vert.A1 Simply Get All Information from Your Cells In Brief The Advantages The Applications The System Technology and Details Service Microscope StandDimensions (W x D x H, including required space for cabling and plugs) Weight EyepiecesAxio Vert.A1Axio Vert.A1 FLAxio Vert.A1 FL-LEDmanual inverted stand, transmitted lightmanual inverted stand, transmitted light and fluorescencemanual inverted stand, transmitted light and LED fluorescence235 x 560 x 560 mm235 x 560 x 560 mm235 x 560 x 560 mm10.5 kg11.7 kg12.3 kgField number 23 (W-Pl 10x/23 br foc), diameter: 30 mm8 9. Axio Vert.A1 Simply Get All InformationObjectivesfrom Your CellsNosepiece turretAxio Vert.A1Axio Vert.A1 FLAxio Vert.A1 FL-LEDAxio Vert.A1Axio Vert.A1 FLAxio Vert.A1 FL-LEDAxio Vert.A1Axio Vert.A1 FLAxio Vert.A1 FL-LEDAxio Vert.A1Axio Vert.A1 FLAxio Vert.A1 FL-LED5x, H DIC M27 man. (2x H, 3x H DIC)ObjectivesA-Plan LD A-Plan LD Plan-NEOFLUAR In Brief Objective magnification1.25x-100x The Advantages Contrast methods (transmitted light) The ApplicationsBrightfield Phase contrast The SystemVAREL PlasDIC Technology and Details ServiceiHMC DIC Illumination (transmitted light) Hal 100 (Halogen)Output: 37 W,, controllability: continuous, 1,5 bis 12VLEDOutput: 3 W, controllability: continuous, 1,5 bis 12VFluorescence HBO 50Power consumption: max. 90 VAHBO 100Power consumption: max. 155 VAHPX 120 CPower consuption: max. 210 VA4-position mount for LED modulesfor reflected light fluorescence4-position reflector turretfor reflected light fluorescence and DIC included with stand optional not possible9 10. Axio Vert.A1 Simply Get All InformationAccessoriesfrom Your CellsTubesAxio Vert.A1Axio Vert.A1 FLAxio Vert.A1 FL-LEDBinocular tube 45, 23 Binocular phototube, left 45, 23 (50:50) Binocular phototube, 45, 23 (50:50) Binocular ergotube, 30-60, 23 In Brief Tubes, Ergo intermediate piecesIntermediate phototube, H=50 mm, left port Ergo intermediate piece, H=25 mm for optimization of viewing height The AdvantagesErgo intermediate piece, H=50 mm for optimization of viewing height The ApplicationsCondensorsLD-condensor 0,3 for slider LD-condensor 0,4 for slider LD-condensor 0,4 for H Ph PlasDIC DIC iHMC The SystemLD-condensor 0,55 for H Ph PlasDIC DIC TechnologyStagesand DetailsSpecimen stage with optional object guide M for various mounting frames M Mechanical stage 130x85 R/L with short coaxial drive for various mounting frames K ServiceGliding stage Z with stage inserts Scanning Stage 130x85 mot P; CAN for various mounting frames K AccessoriesAquastop Mounting frames, heatable mounting frames, incubators Micromanipulation included with stand optional not possible10 11. Axio Vert.A1 Simply Get All InformationOperational datafrom Your CellsArea of useClosed spacesProtection class / protection typeI, IP 20Electrical safetyacc. to DIN EN 61010-1 (IEC 61010-1) allowing for CSA and UL specificationsOvervoltage categoryIIRadio interference suppressionacc. to EN 55011 Class BInterference immunityacc. to DIN EN 61326-1Power supply100 to 240 V AC 10 %Power frequency50 to 60 HzPower consumption internal mains adaptermax. 80 VA In Brief The Advantages The Applications The SystemPower supply for HBO 100and Details ServiceArea of useClosed spacesProtection class / protection typeI, IP 20Power supply Technology100 VAC ... 240 VACPower frequency50/60 HzPower consumption with HBO 100155 VAPower supply for HBO 50 Area of useClosed spacesProtection class / protection typeI, IP 20Power supply100 to 240 V AC 10 %Power frequency50 to 60 HzPower consumption HBO 50max. 90 VAPower supply for HXP 120 C Power supply100 bis 240 V 10 %Power consumption HXP 120 Cmax. 210 VA11 12. Axio Vert.A1 Simply Get All InformationAxio Vert.A1 FL-LEDfrom Your CellsLED-illumination in reflected light with changeable LED modules, wavelengths365, 385, 420, 445, 455, 470, 505, 530, 590, 615, 625 nm or neutral white (540 - 580 nm)LED-classificationLED-risk group 1 acc. DIN EN 62471:2009 In Brief The Advantages The Applications The System Technology and DetailsAxio Vert.A1 FL Wave length400 to 700 nm, peak at 460 nmLED-classificationLED-risk group 1 acc. DIN EN 62471:2009Fuses acc. to IEC 127 Stand Axio Vert.A1T 3,15 A/H, 5x20 mmPower supply for HBO 50T 1,6 APower supply for HBO 100T 2,0 A/H, 5x20 mmPower supply for HAL 100T 5,0 A/H, 5x20 mm Service12