John La Salle - Opening Plenary

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The Barcode Blitz: accelerating the targeted capture of barcode data

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<ul><li>1.The Barcode Blitz: accelerating the targeted capture of barcode data John La Salle, Beth Mantle CSIRO Ecosystem Sciences, Canberra, Australia Jeff Webb, Rodolphe Rougerie, Paul Hebert Biodiversity Institute of Ontario, Guelph Canada</li></ul> <p>2. The Barcode Blitz- ANIC Lepidoptera </p> <ul><li><ul><li>A coordinated assault for targeted and priority capture of barcode data on ANIC Lepidoptera </li></ul></li></ul> <ul><li><ul><li>Has shown it is possible to gather large amounts of data quickly </li></ul></li></ul> <ul><li><ul><li>Built a substantive library for a megadiverse group at continental scale in a short time frame </li></ul></li></ul> <ul><li><ul><li>Fits into / supports </li></ul></li></ul> <ul><li><ul><li><ul><li>Large scale digitisation efforts </li></ul></li></ul></li></ul> <ul><li><ul><li><ul><li>Research outcomes </li></ul></li></ul></li></ul> <ul><li><ul><li><ul><li>Operational outcomes </li></ul></li></ul></li></ul> <ul><li><ul><li><ul><li>Rapid species discovery </li></ul></li></ul></li></ul> <p>3. The Barcode Blitz </p> <ul><li><ul><li>Two visits from BIO / Guelph team to ANIC </li></ul></li></ul> <ul><li><ul><li>5 people x 5 weeks each </li></ul></li></ul> <ul><li><ul><li>Jaclyn McCormick Paul Hebert </li></ul></li></ul> <ul><li><ul><li>Jayme Sones Rodolphe Rougerie </li></ul></li></ul> <ul><li><ul><li>Stephanie deWaard Jeff Webb </li></ul></li></ul> <ul><li><ul><li>Cirsty Carr Jeremy deWaard </li></ul></li></ul> <ul><li><ul><li>Renee Labbee Patrick Strutzenberger </li></ul></li></ul> <ul><li><ul><li>Valerie Levesque-Beaudin Mari Kekkonen </li></ul></li></ul> <p>4. The Barcode Blitz </p> <ul><li><ul><li>In 10 weeks </li></ul></li></ul> <ul><li><ul><li>28,000 specimens processed </li></ul></li></ul> <ul><li><ul><li>8,000 species</li></ul></li></ul> <ul><li><ul><li>Databased, geocoded, imaged on site </li></ul></li></ul> <ul><li><ul><li>Leg taken </li></ul></li></ul> <ul><li><ul><li>Sequenced in Canada </li></ul></li></ul> <p>5. Assembly line processing 6. Assembly line processing 7. Selecting specimens </p> <ul><li><ul><li>Multiple specimens per OTU </li></ul></li></ul> <p>8. Selecting specimens </p> <ul><li><ul><li>From collection to array box </li></ul></li></ul> <p>9. Specimens into array boxes </p> <ul><li><ul><li>Each space numbered </li></ul></li></ul> <ul><li><ul><li>Aligns with number of wells in lysis plate </li></ul></li></ul> <p>10. </p> <ul><li><ul><li>Position of every specimen taken from collection marked </li></ul></li></ul> <p>11. Specimens databased and geocoded 12. Specimens into processing boxes </p> <ul><li><ul><li>Specimens labelled as they are processed </li></ul></li></ul> <ul><li><ul><li><ul><li>BOLD label </li></ul></li></ul></li></ul> <ul><li><ul><li><ul><li>ANIC label </li></ul></li></ul></li></ul> <p>13. Every specimen photographed 14. Leg removed for barcoding 15. </p> <ul><li><ul><li>Each lysis plate gets unique label that ties it back to array box </li></ul></li></ul> <p>16. Specimens back to collection </p> <ul><li><ul><li>Specimens returned to exactly where they were taken </li></ul></li></ul> <p>17. The Barcode Blitz </p> <ul><li><ul><li>In 10 weeks </li></ul></li></ul> <ul><li><ul><li>28,000 specimens processed </li></ul></li></ul> <ul><li><ul><li>8,000 species</li></ul></li></ul> <ul><li><ul><li>Databased, geocoded, imaged on site </li></ul></li></ul> <ul><li><ul><li>Uploaded to BOLD within 24 hours </li></ul></li></ul> <ul><li><ul><li>Leg taken </li></ul></li></ul> <ul><li><ul><li>Sequenced in Canada </li></ul></li></ul> <p>18. The Barcode Blitz- what did we achieve Despite an average age of 30 years,COI sequences were recovered from more than 95% of the specimens 19. The Barcode Blitz- what did we achieve Provided the first continent-wide data set for a megadiverse insect group in Australia within a short period of time (about 90% of named Lepidoptera) Provides a model for inclusion in other initiatives aimed at the high-throughput accumulation of priority data sets.Establishes the great value of existing natural history collections as a basis for fast tracking the development of comprehensive DNA barcode libraries. 20. Despite popular opinion 21. The Barcode Blitz- Australian benefits </p> <ul><li><ul><li>Digital records in ANIC, ALA, BOLD</li></ul></li></ul> <ul><li><ul><li><ul><li>(28,000 databased, geocoded, imaged specimens) </li></ul></li></ul></li></ul> <p>22. The Barcode Blitz- results </p> <ul><li><ul><li>What did we achieve </li></ul></li></ul> <p>23. The Barcode Blitz- results </p> <ul><li><ul><li>What did we achieve </li></ul></li></ul> <p>24. The Barcode Blitz- Australian benefits </p> <ul><li><ul><li>ANIC barcoding blitz activity is our clearest and most positive example of an accelerated and serious attempt to digitise a significant representative sample of a national collection.</li></ul></li></ul> <ul><li><ul><li>Presenting the results in their fullness (including the capability of this process to highlight the range of undescribed diversity) would be a key element in a case to support a major national digitisation activity </li></ul></li></ul> <p>25. One example of international benefit </p> <ul><li><ul><li>A message from South Africa </li></ul></li></ul> <ul><li><ul><li> Note that the mitochondrion sequences we obtained from the groundnut leaf miner in South Africa matched 100% with the soybean mothApproaerema simplexella PS1from Australia on the BOLD system, and that is the reason we are interested in the soybean moth in Australia. </li></ul></li></ul> <p>26. The Barcode Blitz- Australian benefits </p> <ul><li><ul><li>A searchable digital library for Australian Lepidoptera (including images and barcoding)</li></ul></li></ul> <ul><li><ul><li>Foundation for a variety further studies </li></ul></li></ul> <ul><li><ul><li>Facilitate identification </li></ul></li></ul> <ul><li><ul><li>Able to connect life histories </li></ul></li></ul> <ul><li><ul><li>Informs systematics, biodiversity, biosecurity </li></ul></li></ul> <ul><li><ul><li>Foundation for Rapid Taxonomy efforts </li></ul></li></ul> <p>27. Rapid Taxonomy of Australian Xylorictidae </p> <ul><li><ul><li>Generations of investment in morphology (through putative species sorted in the collection) </li></ul></li></ul> <ul><li><ul><li>Estimated 250 new species with most of them already sorted to morphospecies </li></ul></li></ul> <p>28. Rapid Taxonomy of Australian Xylorictidae </p> <ul><li><ul><li>Group with no active specialist (last description in 1964) no revisions on horizon. </li></ul></li></ul> <ul><li><ul><li>Other major museums have made types of Australian xylorictids available for barcoding/study </li></ul></li></ul> <ul><li><ul><li>Become a project for Patrick Strutzenberger (PhD student, Guelph) </li></ul></li></ul> <ul><li><ul><li>DNA barcodes will be used to verify/validate morphospecies in collection </li></ul></li></ul> <ul><li><ul><li>Produce standardized descriptions + images + barcodes (?plus genitalia) </li></ul></li></ul> <ul><li><ul><li>Can progress taxonomic knowledge of this group rapidly </li></ul></li></ul> <ul><li><ul><li>GOAL: 250 species described in 2 years, with 100 done by this time next year </li></ul></li></ul> <p>29. Accelerated Species Discovery </p> <ul><li><ul><li>taxonomist </li></ul></li></ul> <ul><li><ul><li>Accelerated phenomics</li></ul></li></ul> <ul><li><ul><li>Accelerated DNA </li></ul></li></ul> <ul><li><ul><li>Accelerated species discovery and description </li></ul></li></ul> <ul><li><ul><li>Crowdsourcing citizen scientists </li></ul></li></ul> <p>30. Thank you Funding: Genome Canada CSIRO Transformational Biology </p> <ul><li>Bio Team from Guelph: </li></ul> <ul><li><ul><li>Jaclyn McCormick Rodolphe Rougerie </li></ul></li></ul> <ul><li><ul><li>Jayme Sones Jeff Webb </li></ul></li></ul> <ul><li><ul><li>Stephanie deWaard Jeremy deWaard </li></ul></li></ul> <ul><li><ul><li>Cirsty Carr Patrick Strutzenberger </li></ul></li></ul> <ul><li><ul><li>Renee Labbee Mari Kekkonen </li></ul></li></ul> <ul><li><ul><li>Valerie Levesque-Beaudin </li></ul></li></ul> <p>Contact Us Phone: 1300 363 400 or +61 3 9545 2176 Email: enquiries@csiro.auWeb: www.csiro.au </p>