Transcript
Page 1: Microscope Components 2CE-MUZH-4 - · PDF fileS Plan Fluor DIC N1 2 1.2 0.17 0 Microscope Components for Fluorescence Illumination and Transmitted Light Applications Microscope Components

ø23.1

Focal point

30.7

100.

1

27.3

4

17.5

ø53ø42

(Min

. ang

le)

142

582

.8

51.9 85.6

ø51.5

10˚

2112

5.8

ø60

11.3

40.3

151.

3

4.5

46.5

99.8

ø29.

1

ø30

10.5

ø54

48

ø116

ø43.5

80.7

117.8

53

31.8

54.8

7.7

ø40

70˚

M25×0.75ø32

ø10.5ø22

ø27.5ø32.5ø34

30˚

C0.5

1.2(

CG

)

60.4

1

12.8

519

.55

24.7

1.8

(5.1

1)

3.1(

WD

)

5.2

C0.5

C0.5

539

.515

.9

10.9

40X /0.60LWDS Plan Fluor

DIC N1

0.171.22 0

Microscope Components for FluorescenceIllumination and Transmitted Light Applications

Microscope Components for Fluorescence Illumination and Transmitted Light Applications

Page 2: Microscope Components 2CE-MUZH-4 - · PDF fileS Plan Fluor DIC N1 2 1.2 0.17 0 Microscope Components for Fluorescence Illumination and Transmitted Light Applications Microscope Components

2

Nikon's biological microscope units, including objective lenses and eyepiece tubes,

provide high precision and superior operability.

Excellent optical performance through high resolution and aberration correction,

make them optimal for both image acquisition and observation.

This catalog presents the technical data for microscope units that are integrated with a variety of

equipment for biomedical research and testing.

For experimental equipment and inspection systems for biomedical research,

the optical performance of Nikon’s microscope units is always outstanding

Page 3: Microscope Components 2CE-MUZH-4 - · PDF fileS Plan Fluor DIC N1 2 1.2 0.17 0 Microscope Components for Fluorescence Illumination and Transmitted Light Applications Microscope Components

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Contents

System Diagram ���������������������������������������4

CFI60 Objectives

CFI Achromat Series for Brightfield/Phase Contrast (DL)/Apodized Phase Contrast (ADL) �����6

CFI Plan Achromat Series for Brightfield/Phase Contrast (DL) �����������������8

CFI Plan Fluor Series for Brightfield/Phase Contrast (DLL)/Apodized Phase Contrast (ADH) ��� 10

CFI Plan Fluor Series for Phase Contrast (DL) ����������������������� 11

CFI S Plan Fluor ELWD Series for Brightfield/Apodized Phase Contrast (ADM/ADL) ������ 12

Multi-Immersion Objective for Brightfield �������������������������� 12

CFI Plan ApochromatλSeries for Brightfield/Phase Contrast (DM) �������������� 13

CFI Plan Apochromat VC Series ������������������������������ 15

CFI Plan Apochromat IR Lens ������������������������������� 15

CFI Apochromat TIRF Series ������������������������������� 16

CFI SR Series �������������������������������������� 17

CFI HP Series �������������������������������������� 17

CFI Apochromat λS Series �������������������������������� 18

CFI S Fluor Series ������������������������������������ 19

Water Dipping Series ����������������������������������� 20

Nikon Advanced Modulation Contrast (NAMC) Series �������������������� 22

No Cover Glass Objective Lenses ����������������������������� 23

CFI Tube Lens Unit ������������������������������������ 24

Condensers ���������������������������������������� 26

Eyepieces ����������������������������������������� 28

Eyepiece Tubes �������������������������������������� 29

Camera Ports for Ergonomic Binocular Tube ������������������������� 30

Illumination Modules ������������������������������������ 31

Intermediate Modules ������������������������������������ 32

Contact Arm ���������������������������������������� 35

Nosepieces ���������������������������������������� 36

Camera Adapters �������������������������������������� 38

Main Body and Accessories ��������������������������������� 40

For information about the optical values of objectives and the dimensions of units and modules that are not included in this brochure, please contact Nikon.

Page 4: Microscope Components 2CE-MUZH-4 - · PDF fileS Plan Fluor DIC N1 2 1.2 0.17 0 Microscope Components for Fluorescence Illumination and Transmitted Light Applications Microscope Components

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System Diagram

Y-IM Magnification Module

DS-F2.5 F-mount Adapter 2.5x

DS-F F-mount Adapter

V-T Photo Adapter

C-mount TV Adapter 0.35x, 0.45x, 0.6x

C-mount TV AdapterVM2.5x

C-mount TV Adapter VM4x

C-DA C-mount Adapter

C-mount Adapter 0.7x

C-mount Adapter 0.55x

Y-TV TV Tube

Y-TV 0.55 TV Tube

C-TB Binocular Tube C-TF Trinocular Tube F C-TT Trinocular Tube T C-TE2 Ergonomic Binocular Tube

C-TEP2 DSC Port for Ergonomic Binocular Tube

C-TEPF2.5 DSC Port F2.5xfor Ergonomic Binocular Tube

CFI 12.5x CFI 10x CFI UW 10xCFI 15x

CFI60

NI-N7-E Motorized Septuple Nosepiece

NI-ND6-E Motorized DIC Sextuple Nosepiece

NI-N7-I Intelligent Septuple Nosepiece

NI-ND6-I Intelligent DIC Sextuple Nosepiece

D-ND6 DIC Sextuple Nosepiece

C-N6A Sextuple Nosepiece with Analyzer Slot

C-N6 ESD Sextuple Nosepiece ESD

2

3

4

2

3

4

EX.ADJ.

D-FL Epi-Fluorescence Attachment

Illumination Modules P. 31

Main Body and Control Box P. 40~41

Intermediate Modules P. 32~34

C-mount Camera F-mount Camera

Condensers P. 26~27

Nosepieces P. 36~37

Tubes and Ports P. 29~30

Contact Arm P. 35

Camera Adapters P. 38~39

Eyepieces P. 28

ObjectivesCFI Tube Lens Unit P. 6~23P. 24

D H

E

I

F

F

E

J

N

J

I

T

M

D

D

H

H

H

C

C

DD EE

LKM

N

M

K L

T

A

AA A

B

BA

B

CI-FL Epi-Fluorescence Attachment

NI-SAM Standard Arm

NIU-CAMContact Arm

NI-CTLB Control Box B

NI-SSR Substage

NI-CH Condenser Holder

ECLIPSE Ni-U

NI-SRCP Simple Remote Control Pad

F.STOP A.STOP

EX.ADJ.

E

D

E

D

Y-IDP Double Port

NI-FLEI EPI-Fluorescence Attachment

NI-FLT6-E Motorized Epi-fluorescence Cube Turret

NI-FLT6-I Intelligent Epi-fluoresce Cube Turret

NI-FLT6 Epi-fluorescence Cube Turret

C-FL Epi-fl Filter Cube

NI-LH Precentered Lamphouse

NI-BPU Back Port Unit

C-HGFI HG Fiber Illuminator “Intensilight”

C-HGFIE-C HG Controller

C-HGFIE Motorized HG Fiber Illuminator “Intensilight”

C-HGFIB HG 100W Adapter R

C-HGFIF15/30 HG Fiber 1.5 m/3.0 m

Dark Field CondenserOil 1.43 - 1.20

JAPAN

Dark Field CondenserDry 0.95 - 0.80

JAPAN

0.20.40.60.81.01.21.4

Achr-Apl N.A=1.4

Achromat Swing-out Condenser 2-100x

NI-CUD Universal Condenser Dry

C-C Achromat Swing-out Condenser 1-100x

X LWD Condenser

C-C Abbe Condenser

C-C Achromat Condenser

C-C Achromat Aplanatic Condenser

Darkfield Condenser Dry

C-C Slide Achromat Condenser 2-100x

Darkfield Condenser Oil

C-C Phase Contrast Turret Condenser *2

Page 5: Microscope Components 2CE-MUZH-4 - · PDF fileS Plan Fluor DIC N1 2 1.2 0.17 0 Microscope Components for Fluorescence Illumination and Transmitted Light Applications Microscope Components

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Y-IM Magnification Module

DS-F2.5 F-mount Adapter 2.5x

DS-F F-mount Adapter

V-T Photo Adapter

C-mount TV Adapter 0.35x, 0.45x, 0.6x

C-mount TV AdapterVM2.5x

C-mount TV Adapter VM4x

C-DA C-mount Adapter

C-mount Adapter 0.7x

C-mount Adapter 0.55x

Y-TV TV Tube

Y-TV 0.55 TV Tube

C-TB Binocular Tube C-TF Trinocular Tube F C-TT Trinocular Tube T C-TE2 Ergonomic Binocular Tube

C-TEP2 DSC Port for Ergonomic Binocular Tube

C-TEPF2.5 DSC Port F2.5xfor Ergonomic Binocular Tube

CFI 12.5x CFI 10x CFI UW 10xCFI 15x

CFI60

NI-N7-E Motorized Septuple Nosepiece

NI-ND6-E Motorized DIC Sextuple Nosepiece

NI-N7-I Intelligent Septuple Nosepiece

NI-ND6-I Intelligent DIC Sextuple Nosepiece

D-ND6 DIC Sextuple Nosepiece

C-N6A Sextuple Nosepiece with Analyzer Slot

C-N6 ESD Sextuple Nosepiece ESD

2

3

4

2

3

4

EX.ADJ.

D-FL Epi-Fluorescence Attachment

Illumination Modules P. 31

Main Body and Control Box P. 40~41

Intermediate Modules P. 32~34

C-mount Camera F-mount Camera

Condensers P. 26~27

Nosepieces P. 36~37

Tubes and Ports P. 29~30

Contact Arm P. 35

Camera Adapters P. 38~39

Eyepieces P. 28

ObjectivesCFI Tube Lens Unit P. 6~23P. 24

D H

E

I

F

F

E

J

N

J

I

T

M

D

D

H

H

H

C

C

DD EE

LKM

N

M

K L

T

A

AA A

B

BA

B

CI-FL Epi-Fluorescence Attachment

NI-SAM Standard Arm

NIU-CAMContact Arm

NI-CTLB Control Box B

NI-SSR Substage

NI-CH Condenser Holder

ECLIPSE Ni-U

NI-SRCP Simple Remote Control Pad

F.STOP A.STOP

EX.ADJ.

E

D

E

D

Y-IDP Double Port

NI-FLEI EPI-Fluorescence Attachment

NI-FLT6-E Motorized Epi-fluorescence Cube Turret

NI-FLT6-I Intelligent Epi-fluoresce Cube Turret

NI-FLT6 Epi-fluorescence Cube Turret

C-FL Epi-fl Filter Cube

NI-LH Precentered Lamphouse

NI-BPU Back Port Unit

C-HGFI HG Fiber Illuminator “Intensilight”

C-HGFIE-C HG Controller

C-HGFIE Motorized HG Fiber Illuminator “Intensilight”

C-HGFIB HG 100W Adapter R

C-HGFIF15/30 HG Fiber 1.5 m/3.0 m

Dark Field CondenserOil 1.43 - 1.20

JAPAN

Dark Field CondenserDry 0.95 - 0.80

JAPAN

0.20.40.60.81.01.21.4

Achr-Apl N.A=1.4

Achromat Swing-out Condenser 2-100x

NI-CUD Universal Condenser Dry

C-C Achromat Swing-out Condenser 1-100x

X LWD Condenser

C-C Abbe Condenser

C-C Achromat Condenser

C-C Achromat Aplanatic Condenser

Darkfield Condenser Dry

C-C Slide Achromat Condenser 2-100x

Darkfield Condenser Oil

C-C Phase Contrast Turret Condenser *2

Page 6: Microscope Components 2CE-MUZH-4 - · PDF fileS Plan Fluor DIC N1 2 1.2 0.17 0 Microscope Components for Fluorescence Illumination and Transmitted Light Applications Microscope Components

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CFI60 ObjectivesCFI Achromat Series for Brightfield/Phase Contrast (DL)Apodized Phase Contrast (ADL)Correction of chromatic aberration, spherical aberration and coma has been dramatically improved, with significantly better image flatness.

60.0

6

4.5

19.9

10

ø25

ø27

M25×0.75

C1

30˚

4 X /0.10∞/- WD 30

CFI Achromat 4x

M25×0.75

ø13.5ø16ø25

ø23.8ø27

4.5

60.0

6

7(W

D)

4.5

10

0.17

(CG

)

(52.

89)

36.5 10 X /0.25

∞/- WD 7.0

CFI Achromat 10x CFI Achromat DL 10x

M25×0.75

60.0

60.

17(C

G)

ø27ø23.8

ø25ø17ø15ø8.2

45˚

4.5

3.9(

WD

)

1032

10(3

.99)

45˚

20X/0.40LWD∞/0.17 WD 3.9

CFI Achromat LWD 20x CFI Achromat LWD DL 20x

ø7ø13ø17ø20ø25

60.0

6

ø27

M25×0.75

45˚

35˚

34.5

3.7

83

105

40X /0.65WD 0.65∞/0.17

CFI Achromat 40x CFI Achromat DL 40x

M25×0.75

ø27

ø7ø13ø17ø20ø25

ø23.8

35˚

(4.0

9)3

42.5

105

8

0.17

(CG

)0.

3(W

D)

60.0

6

45˚

60 X /0.80∞/0.17 WD 0.3

CFI Achromat 60x

M25×0.75

ø5.3ø13

ø16.6ø20ø25

60.0

6

ø27

20˚

45˚

58

34.5

105

2.16

100 X /1.25oil∞/0.17 WD 0.23

CFI Achromat 100x Oil CFI Achromat DL 100x Oil

Page 7: Microscope Components 2CE-MUZH-4 - · PDF fileS Plan Fluor DIC N1 2 1.2 0.17 0 Microscope Components for Fluorescence Illumination and Transmitted Light Applications Microscope Components

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M25×0.75

60.4

13.

111.

2

4.5

1036

6(4

.1)

ø27ø23.8

ø25ø17ø8.2

45˚45˚

Ph1 DL∞/ WD 3.11.2

20 X /0.40

M25×0.75

ø27.5ø23.8

ø29.5

ø30.5ø27.5ø19ø17

ø5.3ø13 0.

170.

2360

.06

531

4.53.5

85.8

4.7

(2.1

6)

0.5-1.25oil lris100 X

∞/0.17 WD 0.23

60.4

1

ø23.8ø27

ø13.5ø16ø25

4.5

1036

.54.

5

(53)

1.2

6.2(

WD

)

45̊

M25×0.75

Ph1 ADL∞/ WD 6.21.2

10 X /0.25

60.4

11.

2

ø23.8ø27

1033

.75.

84.

14.

5

ø12.1ø14.6ø19

ø21.5ø25

45̊

M25×0.75

2.1(

WD

)3.5

Ph1 ADL∞/ WD 2.11.2

40 X /0.55

CFI Achromat LWD DL 20x FCFI Achromat LWD ADL 20x F

CFI Achromat 100x Oil with iris diaphragm

CFI Achromat ADL 10x CFI Achromat LWD ADL 40x F

M25×0.754.5

60.4

11.

22.

1

(5.1

)5

252.

6

ø12ø20

ø25.5

ø34ø32

ø23.8

ø32

40˚

19.4

∞/ WD 2.1

0.41.6 1.2 0.8 02

0-2

40 X /0.55

CFI Achromat LWD 40x C CFI Achromat LWD DL 40x C

CFI Achromat LWD ADL 40x C

Code.No Objectives NAW.D. (mm)

Physical depthof focus (µm)

Actual fieldof view (ømm)

Weight (g) Remarks Phase ring

MRP00042 CFI Achromat 4x 0.10 30.0 27.5 5.5 72

MRP00102 CFI Achromat 10x 0.25 7.0 4.4 2.2 85

MRP20102 CFI Achromat DL 10x 0.25 7.0 4.4 2.2 85 Ph1

MRP40102 CFI Achromat ADL 10x 0.25 6.2 4.4 2.2 85 Cover glass thickness 1.2mm Ph1

MRP00202 CFI Achromat LWD 20x 0.40 3.9 1.72 1.1 90

MRP20202 CFI Achromat LWD DL 20x 0.40 3.9 1.72 1.1 90 Ph1

MRP00402 CFI Achromat 40x 0.65 0.65 0.65 0.55 130 Spring-loaded

MRP20402 CFI Achromat DL 40x 0.65 0.65 0.65 0.55 130 Spring-loaded Ph2

MRP00602 CFI Achromat 60x 0.80 0.3 0.43 0.367 130 Spring-loaded

MRP01902 CFI Achromat 100x Oil 1.25 0.23 0.27 0.22 130 Spring-loaded

MRP21902 CFI Achromat DL 100x Oil 1.25 0.23 0.27 0.22 130 Spring-loaded Ph3

MRP02902 CFI Achromat 100x Oil with iris diaphragm 0.5-1.25 0.23 0.27 0.22 130 Spring-loaded

MRP26202 CFI Achromat LWD DL 20x F 0.40 3.1 1.72 1.1 90 Cover glass thickness 1.2mm Ph1

MRP46202 CFI Achromat LWD ADL 20xF 0.40 3.1 1.72 1.1 90 Cover glass thickness 1.2mm Ph1

MRP46402 CFI Achromat LWD ADL 40xF 0.55 2.1 0.91 0.55 90 Cover glass thickness 1.2mm Ph1

MRP05422 CFI Achromat LWD 40x C 0.55 2.7–1.7 0.91 0.55 195 Cover glass correction 0-2mm

MRP25422 CFI Achromat LWD DL 40x C 0.55 2.7–1.7 0.91 0.55 195 Cover glass correction 0-2mm Ph2

MRP45422 CFI Achromat LWD ADL 40xC 0.55 2.7–1.7 0.91 0.55 195 Cover glass correction 0-2mm Ph2

Page 8: Microscope Components 2CE-MUZH-4 - · PDF fileS Plan Fluor DIC N1 2 1.2 0.17 0 Microscope Components for Fluorescence Illumination and Transmitted Light Applications Microscope Components

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CFI60 ObjectivesCFI Plan Achromat Series for Brightfield/Phase Contrast (DL)Nikon's CFI Plan Achromat series provides incredible image flatness, with chromatic aberration corrected throughout the entire visible spectrum. These objectives are suitable not only for observation but also for capturing images.

60.0

6

4610

.75

ø31

ø30

M25×0.75

1X /0.04Plan UW

∞/-WD 3.2

CFI Plan Achromat UW 1x

60.0

652.4

5

5

ø20

ø30

M25×0.75

2X /0.06∞/-WD 7.5

Plan UW

CFI Plan Achromat UW 2x

60.0

6

19.9

104.

5

ø25

ø27

M25×0.75

4X /0.∞/-WD 3

Plan

CFI Plan Achromat 4x

60.0

6372.

45

10

ø19

ø12

ø25

ø27

M25×0.75

10X /0.25∞/-WD 10.5

Plan

45˚

45˚

CFI Plan Achromat 10x CFI Plan Achromat DL 10x

M25×0.75

60.0

6

1.22

(WD

)0.

17(C

G)

ø23.8

ø27

ø25

ø13

ø7

ø17

ø20

4.5

10 0.6

7.5

2.5

322

(4.6

7)

30˚

20 X /0.40Plan

∞/0.17 WD 1.2

CFI Plan Achromat 20x CFI Plan Achromat DL 20x

M25×

0.75

ø23.8

ø27

ø6

44.5

(2.5

)

0.17

(CG

)

60.0

6

10

52

55.5

(59.

33)

ø12.8

ø17

ø20

ø25

2

40 X /0.65Plan

∞/0.17 WD 0.56

CFI Plan Achromat 40x CFI Plan Achromat DL 40x

Page 9: Microscope Components 2CE-MUZH-4 - · PDF fileS Plan Fluor DIC N1 2 1.2 0.17 0 Microscope Components for Fluorescence Illumination and Transmitted Light Applications Microscope Components

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M25×0.75

0.17

ø23.8

ø14.5

ø19.5

ø22

60.0

6

ø27.5

5

ø5.5

0.18

49.7

5

4.25

45˚

30˚

1.5

(4.1

7)

50 X /0.90oil

Plan

∞/- WD 0.35

CFI Plan Achromat 50x Oil

M25×0.75

ø5.87

ø16

ø21

ø23.8

ø27.5

60.0

6

0.17

(CG

)0.

22(O

il)

47

57.5

(59.

67)

5

2.7

20˚

ø22.1

100XA/1.25Plan

∞/0.17 WD 0.2

CFI Plan Achromat 100x Oil CFI Plan Achromat DL 100x Oil

Code.No Objectives NAW.D.(mm)

Physical depth of focus (µm)

Actual fieldof view (ømm)

Weight (g) Remarks Phase ring

MRL00012 CFI Plan Achromat UW 1x 0.04 3.2 171.88 25 180

MRL00022 CFI Plan Achromat UW 2x 0.06 7.5 76.39 12.5 110

MRL00042 CFI Plan Achromat 4x 0.10 30.0 27.5 6.25 65

MRL00102 CFI Plan Achromat 10x 0.25 10.5 4.4 2.5 85

MRL20102 CFI Plan Achromat DL 10x 0.25 10.5 4.4 2.5 85 Ph1

MRL00202 CFI Plan Achromat 20x 0.40 1.2 1.7 1.25 85

MRL20202 CFI Plan Achromat DL 20x 0.40 1.2 1.72 1.25 85 Ph1

MRL00402 CFI Plan Achromat 40x 0.65 0.56 0.65 0.625 115 Spring-loaded

MRL20402 CFI Plan Achromat DL 40x 0.65 0.56 0.65 0.625 115 Spring-loaded Ph2

MRL01502 CFI Plan Achromat 50x Oil 0.90 0.35 0.51 0.44 160 Spring-loaded No cover grass

MRL01903 CFI Plan Achromat 100x Oil 1.25 0.2 0.27 0.22 160 Spring-loaded

MRL21903 CFI Plan Achromat DL 100x Oil 1.25 0.2 0.27 0.22 160 Spring-loaded Ph3

Page 10: Microscope Components 2CE-MUZH-4 - · PDF fileS Plan Fluor DIC N1 2 1.2 0.17 0 Microscope Components for Fluorescence Illumination and Transmitted Light Applications Microscope Components

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CFI60 ObjectivesCFI Plan Fluor Series for Brightfield/Phase Contrast (DLL)/Apodized Phase Contrast (ADH)

M25×0.75

ø16.5

45˚

(42.

69)

4

ø3017

.2(W

D)

0.17

(CG

)

60.0

6

4 X /0.13Plan Fluor

∞/- WD 17.2

CFI Plan Fluor 4x

M25×0.75

0.17

(CG

)

ø23.8ø28.2ø30

ø18

16.0

9(W

D)

60.0

6

45˚

(42.

4)

1.4

5

C0.6

10X /0.30DIC L/N1

Plan Fluor

∞/0.17 WD 16

JAPAN

CFI Plan Fluor 10x CFI Plan Fluor DLL 10x

M25×0.75ø23.8

ø28

ø8.5

ø15.5

45˚

5

0.17

(CG

)

60.0

6

2.1(

WD

)

ø20.5

51

45˚

(6.7

9)

20 X /0.50

Plan Fluor

∞/0.17 WD 2.1

CFI Plan Fluor 20x CFI Plan Fluor DLL 20x

M25×0.75ø23.8

ø27.5

ø28.2

ø30

45˚

5

156

.2

45˚

ø10

ø19

45˚

0.17

(CG

)0.

66(W

D)

60.0

6

(59.

23) 40X /0.75

DIC M/N2

Plan Fluor

∞/0.17 WD 0.66

JAPAN

CFI Plan Fluor 40x CFI Plan Fluor DLL 40x

M25×0.75

1240

4

(59.

61)

0.17(

CG)

0.28

(WD

)

60.0

6

ø6.92

22.5˚

ø22

ø25.8

ø28

C0.5

ø33.5

ø23.8

45˚

ø28

45˚82˚

5

40X /1.30oil

Plan Fluor

∞/0.17 WD 0.24

CFI Plan Fluor 40x Oil

M25×0.75ø23.8

ø5ø19.3ø21.5

40˚

70˚

60˚

0.17

(CG

)

60.0

6

5

32.5

59 2.1

ø30.5

ø31.5

ø26ø28

0.35

(WD

)

7.4

(59.

52)

60X/0.85Plan Fluor

/0.11-0.23 WD0.40-0

CFI Plan Fluor 60x

Featuring an extra-high transmission rate, especially in the ultraviolet wavelength, and flatness of field, this series is designed for fluorescence observation and imaging. These objectives can function as multi-purpose objectives for brightfield, fluorescence, simple polarizing, and DIC observations.

Code.No Objectives NAW.D.(mm)

Physical depth of focus (µm)

Actual fieldof view (ømm)

Weight (g) Remarks Phase ring

MRH00041 CFI Plan Fluor 4x 0.13 17.2 16.27 6.25 110

MRH00101 CFI Plan Fluor 10x 0.30 16.0 3.06 6.25 120

MRH10101 CFI Plan Fluor DLL 10x 0.30 16.0 3.06 2.5 120 Ph1

MRH00201 CFI Plan Fluor 20x 0.50 2.1 1.1 1.25 175

MRH10201 CFI Plan Fluor DLL 20x 0.50 2.1 1.1 1.25 175 Ph1

MRH00401 CFI Plan Fluor 40x 0.75 0.66 0.49 0.625 210 Spring-loaded

MRH10401 CFI Plan Fluor DLL 40x 0.75 0.66 0.49 0.625 210 Spring-loaded Ph2

MRH01401 CFI Plan Fluor 40x Oil 1.30 0.24 0.25 0.625 230 Spring-loaded,With stopper

MRH00602 CFI Plan Fluor 60x 0.85 0.4-0.31 0.38 0.417 265Spring-loadedCover glass correction 0.11–0.23mm

MRH02601 CFI Plan Fluor 60x Oil with iris diaphragm 0.50-1.25 0.22 0.27 0.417 230 Spring-loaded

MRH01902 CFI Plan Fluor 100x Oil 1.30 0.16 0.25 0.25 250 Spring-loaded,With stopper

MRH11902 CFI Plan Fluor DLL 100x Oil 1.30 0.16 0.25 0.25 250 Spring-loaded,With stopper Ph3

MRH41902 CFI Plan Fluor ADH 100x Oil 1.30 0.16 0.25 0.25 250 Spring-loaded,With stopper Ph3

MRH02902 CFI Plan Fluor 100x Oil with iris diaphragm 0.5–1.3 0.16 0.25 0.25 285 Spring-loaded

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M25×0.75

ø30

ø23.8

ø27.7

ø26

ø20.8

ø17

ø6.1

60.0

6

0.17

(CG

)0.

16(O

il)

5

0.8

45˚

20˚4.5

(59.

71)

50.7 100X/1.30oil

∞/0.17 WD 0.16

Plan Fluor

M25×0.75

ø8ø29.2ø30.3ø32ø35

ø30ø33

ø34.5ø36

30˚75

˚

45˚

5

0.5

517

55

9.5

2.5

12(3

.16)

0.17

(CG

)

60.0

6

0.23

(oil)

ø27.5C0.5

60X/0.5-1.25oil Iris

1.25 0.5

Plan Fluor

∞/0.17 WD 0.22

CFI Plan Fluor 100x Oil CFI Plan Fluor DLL 100x OilCFI Plan Fluor ADH 100x Oil

M25×0.75

ø5.2

ø23.4

ø32

ø32.4

ø33.4

ø32

ø23.8

38.8

3.5(5

9.7)

3.5

2.7 6.5

2.2

0.17

(CG

)0.

16(O

il)

60.0

6

45˚

17˚

5

ø19.8

ø27

7.1

100X/0.5-1.3oil IrisPlan Fluor

∞/0.17 WD 0.16

CFI Plan Fluor 100x Oil with iris diaphragm

CFI Plan Fluor 60x Oil with iris diaphragm

4X / 0.13 Plan Fluor

PhL DL ∞/1.2 WD 16.5

M25×0.75

ø30

60.4

1

42.8

55.

3

45˚

CFI Plan Fluor DL 4x

10X / 0.30 Plan Fluor

Ph1 DL ∞/1.2 WD 15.2

M25×0.75

ø30

60.4

1

1.2

445.

3

45˚

6

CFI Plan Fluor DL 10x

CFI Plan Fluor Series for Phase Contrast (DL)These objectives have more contrast than the DLL objectives for enhanced contrast with tissue culture specimens. These objectives are designed for use on inverted microscopes and therefore their markings are upside-down for easy reading.

Code.No Objectives N.A.W.D.(mm)

Physical depth of focus (µm)

Actual fieldof view (ømm)

Weight (g) Remarks Phase ring

MRH20041 CFI Plan Fluor DL 4x 0.13 16.5 16.27 6.25 105 Cover glass thickness 1.2mm PhL

MRH20101 CFI Plan Fluor DL 10x 0.30 15.2 3.06 2.50 120 Cover glass thickness 1.2mm Ph1

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CFI60 Objectives

ø8

ø33

ø28

1910

.412

.514

5.3

3.7

M25×0.75

20X / 0.75 Mlmm Plan Fluor

∞ / - WD 0.35

Oil G W0.17 0

ø36

ø34.5

ø30.6

30˚

40˚

CFI Plan Fluor 20x MI

M25×0.75ø32

ø16ø25.5ø32.5ø34

35˚

C0.8

5

12.819

.55

22.2

60.4

1

1.2(

CG

)7.

4(W

D)

C0.5

C0.5

(5.1

1)

20X /0.45LWDS Plan Fluor

∞/0-2 Ph1 ADM

CFI S Plan Fluor ELWD 20x C CFI S Plan Fluor ELWD ADM 20x C

M25×0.75

Front focal point

Back focal pointPupil

position

ø32

ø10.5

ø22

ø27.5

ø32.5

ø34

30˚

C0.5

1.2(

CG

)

60.4

1

12.8

519

.55

24.7

1.8

(5.1

1)

3.1(

WD

)

5.2

C0.5

C0.5

539

.515

.9

10.9

40X /0.60LWDS Plan Fluor

DIC N1

0.171.22 0

CFI S Plan Fluor ELWD 40x C CFI S Plan Fluor ELWD ADM 40x C

M25×0.75

ø8.8

ø17

ø19.2

ø22

ø29.5

ø31.5

35˚35˚

C0.5

C0.5

ø309˚

5

3.8

17.5

5

5.2

28

3.06

(3.8

5) 1.2(

CG

)1.

8(W

D)

60.4

1

60X /0.70WDS Plan Fluor

∞/0.1-1.3 WD2.6-1.8

0.171.0 0.7 0.41.2

CFI S Plan Fluor ELWD 60x C CFI S Plan Fluor ELWD ADL 60x C

Multi-Immersion Objective for Brightfield

Newly developed broadband multilayer coating realizes high transmittance from near-ultraviolet to near-infrared wavelengths, with improved chromatic correction. The correction collar ring allows these objectives to be used with a diverse range of culture vessels and specimen thicknesses. High-quality images with no aberrations can be obtained under a broad range of illumination techniques.

This multi-purpose immersion objective has a higher numerical aperture and superior contrast. The use of immersion media helps eliminate surface reflections and provides an image with higher contrast. It is an excellent objective for fluorescence, brightfield, and DIC observation techniques. This unique objective has a correction collar that allows it to be used with immersion oil, glycerin, and water (with and without a cover glass).

CFI S Plan Fluor ELWD Series for Brightfield/Apodized Phase Contrast (ADM/ADL)

Code.No Objectives NAW.D.(mm)

Physical depth of focus (µm)

Actual fieldof view (ømm)

Weight (g) Remarks Phase ring

MRH08230 CFI S Plan Fluor ELWD 20x C 0.45 8.2–6.9 1.36 1.1 195 Cover glass correction 0–2mm

MRH48230 CFI S Plan Fluor ELWD ADM20x C 0.45 8.2–6.9 1.36 1.1 195 Cover glass correction 0–2mm Ph1

MRH08430 CFI S Plan Fluor ELWD 40x C 0.60 3.6–2.8 0.76 0.55 195 Cover glass correction 0–2mm

MRH48430 CFI S Plan Fluor ELWD ADM40x C 0.60 3.6–2.8 0.76 0.55 195 Cover glass correction 0–2mm Ph2

MRH08630 CFI S Plan Fluor ELWD 60x C 0.70 2.6–1.8 0.56 0.367 210 Cover glass correction 0.1–1.3mm

MRH48600 CFI S Plan Fluor ELWD ADL60x C 0.70 2.6–1.8 0.56 0.367 210 Cover glass correction 0.1–1.3mm Ph2

Code.No Objective NAW.D.(mm)

Physical depth of focus (µm)

Actual fieldof view (ømm)

Weight (g) Remarks

MRH07241CFI Plan Fluor 20x MI

0.75 0.35(Oil) 0.74 1.25 300

Multi-immersionOil-glycerine-waterSpring-loadedCover glass correction 0–0.17mm

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M25×0.75ø23.8

ø30

ø19

45˚

5(5

1.39

)

60.0

6

0.17

(CG

)8.

5(W

D)

ø29

0.5

C0.5

2X /0.10

Plan Apo λ

∞/- WD 8.5

CFI Plan Apochromatλ2x

M25×0.75

5(3

9.89

)

ø18

30˚ 60

.06

20(W

D)

0.17

(CG

)

ø23.8

ø30

ø28.2

0.8

4X /0.20

Plan Apo λ

∞/- WD 20

CFI Plan Apochromatλ4x

M25×0.75ø23.8

ø30

5(5

5.89

)

60.0

6

4(W

D)

0.17

(CG

)

45˚

ø15

ø27.7

0.8

10X /0.45

Plan Apo λ

∞/0.17 WD 4.0

CFI Plan Apochromatλ10x

M25×0.75

0.17

(CG

)

60.0

6

ø27

ø23.8

ø25.8

ø21.8

5

3.5

40

ø27

ø28

0.5

49.5

ø6.5

45˚

(58.

89)

1(W

D)

60˚

ø31

ø32.5

45˚

20X /0.75

Plan Apo λ

∞/0.17 WD 1.0

CFI Plan Apochromatλ20x CFI Plan ApochromatλDM 20x

M25×0.75ø23.8

ø27

5

0.5

7.5

ø18.8

0.17

(CG

)

60.0

6

18.9

11.1

ø8

40˚

16.8

40˚

ø21

70˚

ø33

ø34.5

(59.

68)

0.21

(WD

)

ø28.5

ø30.8

40X /0.95Plan Apo λ

∞/0.11-0.23

0.11 0.17 0.23

WD0.25-0.17

CFI Plan Apochromaλ40x CFI Plan ApochromatλDM 40x

M25×0.75ø23.8

ø27

0.5

5

70˚

ø6.5

0.17

(CG

)

60.0

6

18

40˚

40˚

ø18.5

0.15

(WD

)

(59.

74)

ø28.5

ø33

ø34.5

ø30.6

811

.616

.4

ø21

60X /0.95Plan Apo λ

∞/0.11-0.23 WD0.20-0.11

CFI Plan Apochromatλ60x CFI Plan ApochromatλDM 60x

The ultra-low, refractive index coating technology “Nano Crystal Coat” enables the λ series’ remarkably high transmission throughout a broad range of wavelengths up to the near-IR region. As chromatic aberrations are corrected throughout a wavelength range from visible to near infrared, bright, high-contrast images are captured during long-wavelength imaging, which is less phototoxic to live-cells. Moreover, the unmatched chromatic aberration correction, resolution and image flatness of the λ series ensure the capture of high-quality brightfield images.

CFI Plan ApochromatλSeries for Brightfield/Phase Contrast (DM)

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M25×0.75

0.17

(CG

)

60.0

6

ø25.8

5

3.5

40

ø27

0.13

(WD

)

(59.

76)

35˚

ø9.17

ø23.8

ø27

ø28

0.5

60˚

ø31

32.5

45˚

55.8

60X /1.40oil

Plan Apo λ

∞/0.17 WD 0.13

CFI Plan Apochromatλ60x Oil CFI Plan ApochromatλDM 60x Oil

M25×0.75

0.17

(CG

)0.

13(W

D)

60.0

6

ø25.8

ø9.17

5(5

9.76

)

3.5

40

ø27

45˚

35˚

ø23.8

ø27

ø28

0.5

60˚

ø32.5

ø31

56

100X /1.45oil

Plan Apo λ

∞/0.17 WD 0.13

CFI Plan Apochromatλ100x Oil CFI Plan ApochromatλDM 100x Oil

CFI60 ObjectivesCFI Plan Apochromatλ Series for Brightfield/Phase Contrast (DM)

Code.No Objectives NAW.D. (mm)

Physical depth of focus (µm)

Actual fieldof view (ømm)

Weight (g) Remarks Phase ring

MRD00025 CFI Plan Apochromat λ2x 0.10 8.5 27.50 12.5 180

MRD00045 CFI Plan Apochromat λ4x 0.20 20.0 6.88 6.25 180

MRD00105 CFI Plan Apochromat λ10x 0.45 4.0 1.36 2.5 220

MRD00205 CFI Plan Apochromat λ20x 0.75 1.0 0.49 1.25 240 Spring-loaded

MRD30205 CFI Plan Apochromat λDM20x 0.75 1.0 0.49 1.25 240 Spring-loaded Ph2

MRD00405 CFI Plan Apochromat λ40x 0.950.21

(0.25-0.17)0.30 0.625 270

Spring-loadedCover glass correction 0.11–0.23mm

MRD30405 CFI Plan Apochromat λDM40x 0.950.21

(0.25-0.17)0.30 0.625 270

Spring-loadedCover glass correction 0.11–0.23mm

Ph2

MRD00605 CFI Plan Apochromat λ60x 0.950.15

(0.20-0.11)0.30 0.417 285

Spring-loadedCover glass correction 0.11–0.23mm

MRD30605 CFI Plan Apochromat λDM60x 0.950.15

(0.20-0.11)0.30 0.417 285

Spring-loadedCover glass correction 0.11–0.23mm

Ph2

MRD01605 CFI Plan Apochromat λ60x Oil 1.40 0.13 0.21 0.417 285 Spring-loaded

MRD31605 CFI Plan Apochromat λDM60x Oil 1.40 0.13 0.21 0.417 285 Spring-loaded Ph3

MRD01905 CFI Plan Apochromat λ100x Oil 1.45 0.13 0.20 0.25 245 Spring-loaded

MRD31905 CFI Plan Apochromat λDM100x Oil 1.45 0.13 0.20 0.25 245 Spring-loaded Ph3

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M25×0.75

60.0

6

0.17

(CG

)

ø23.8ø27.5ø30ø33

10˚

5

0.5

48.5

45˚

4(5

.828

)

30˚

C0.5

ø7ø21.4ø25.8ø27.3

1.06

(WD

)

20X /0.75DIC N2

Plan Apo VC

∞/0.17 WD 1.0

CFI Plan Apochromat VC 20x

M25×0.75ø31ø33

ø34.5

ø8

ø26ø31.4

56.

258.

75(3

.39)

22.5

0.17

(CG

)0.

3(w

ater

)

60.0

6±0.

01

8.7

6.2

3.8

ø22.8

60XA/1.20 WIPlan Apo VC

∞/0.15-0.18 WD0.31-0.28

15 16 17 18 19

CFI Plan Apochromat VC 60x WI

M25×0.75ø23.8

ø6.9

ø28

25˚

55.5

5

ø22

0.17

(CG

)0.

13(W

D)

60.0

6

45

ø35

C2

31.6

ø27.5ø30

0.5

C1

(59.

74) 100X /1.40oil

Plan Apo VC

∞/0.17 DIC N2

JAPAN

CFI Plan Apochromat VC 100x Oil

CFI Plan Apochromat VC Series

CFI Plan Apochromat IR Lens

These objectives provide perfect correction of chromatic aberrations in the visible light range and excellent resolution throughout the view field, and are the perfect choice for multi-stained, fluorescence specimens, and for brightfield and DIC observation. In addition to the correction range of the conventional Plan Apochromat series (435–660nm), axial chromatic aberration has been corrected up to the violet range (405nm), making these objectives highly effective for confocal applications.The 60x water-immersion type features high spectral transmittance, even in the 360nm wavelength ultra-violet range, making it perfect for the fluorescence observation of living organisms.

With the world’s highest NA (1.27) for a 60x water immersion objective, this lens achieves a high level of resolution and sharp image acquisition. It corrects chromatic aberration up to 1,064 nm.

0.17

(CG

)

60.0

6

5

ø9

ø25.1ø33ø36

ø34.5M25×0.7510˚

45˚

3

ø20

0.17

(wat

er) 6.9

20.5

20.1

6

ø23.8

9.5

20˚

(3.1

5)

60Plan Apo IR

X /1.27 WI

0.19 0.17 0.15

∞/0.15-0.19 WD0.18-0.16

CFI Plan Apochromat IR 60x Wl

Code.No Objectives NAW.D.(mm)

Physical depth of focus (µm)

Actual fieldof view (ømm)

Weight (g) Remarks

MRD70200 CFI Plan Apochromat VC20x 0.75 1.0 0.49 1.25 170 Spring-loaded

MRD07602 CFI Plan Apochromat VC60x WI 1.200.29

(0.31–0.28)0.25 0.367 220

Spring-loadedCover glass correction 0.15–0.18mm

MRD01901 CFI Plan Apochromat VC100x Oil 1.40 0.13 0.21 0.25 300 Spring-loaded

Code.No Objectives (magnification) NAW.D.(mm)

Physical depth of focus (µm)

Actual fieldof view (ømm)

Weight (g) Remarks

MRD07650 CFI Plan Apochromat IR60x Wl 1.27 0.18–0.16 0.23 0.367 250 Spring-loadedCover glass correction 0.15–0.19mm

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M25×0.75

ø9

ø29

ø32

ø23.8

ø27.8

ø31.5

ø33.5

C0.3

C1

5

0.3

16.2

80˚

827

5(3

.2)

C0.5

C1

0.17

(CG

)0.

13(

Oil)

60.0

6

28˚

35.5

60X /1.49oil

23˚CCG 0.19 0.17 0.13

Apo TIRF

∞/0.13-0.21 WD 0.12

CFI Apochromat TIRF 60x Oil

M25×0.75

60.0

6

ø35.5

0.17

(CG

)

ø9

ø29

ø32

C0.3

C1

(3.2

)5

258

18.2

32˚

50.3

ø33.5

ø23.8

ø27.8

ø31.5

80˚

C0.5

C1

0.13

(Oil)

ø27

1.5

45˚ø23

100X /1.49oil

23˚CCG 0.19 0.17 0.13

Apo TIRF

∞/0.13-0.20 WD 0.12

CFI Apochromat TIRF 100x Oil

CFI Apochromat TIRF SeriesBecause of the unprecedented high NA of 1.49—for use with a standard coverslip and immersion oil—these objectives enable the acquisition of high-resolution, high S/N ratio images during TIRF observation, epi-fluorescence and confocal observation, as well as DIC observation. The spherical aberration correction ring reduces deterioration in image quality caused by deviations in cover glass thickness or temperature fluctuations, and provides optimal optical performance at both 23°C and 37°C. The 100x objective can be optimally applied for laser tweezers microscopy.

CFI60 Objectives

Code.No Objectives NAW.D.(mm)

Physical depth of focus (µm)

Actual fieldof view (ømm)

Weight (g) Remarks

MRD01691 CFI Apochromat TIRF 60x Oil 1.49 0.12 0.19 0.367 265 Cover glass correction0.13–0.19mm (23C˚)0.15–0.21mm (37C˚)

MRD01991 CFI Apochromat TIRF 100x Oil 1.49 0.12 0.19 0.18 275 Cover glass correction0.13–0.19mm (23C˚)0.14–0.20mm (37C˚)

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M25×0.75

0.17

(CG

)

60.0

6

5ø9

ø33

ø36

ø34.5

10˚

ø20

0.17

(wat

er)

20.1

6

ø23.8

9.5

ø26.6ø28.5

1.9

ø24.7

45˚

(3.2

2)

20˚

60X /1.27 WISR Plan Apo IR

0.19 0.17 0.15

∞/0.15-0.19 WD0.18-0.16

CFI SR Plan Apochromat IR 60x Wl

M25×0.75

60.0

6

ø35.5

0.17

(CG

)

ø9

ø29ø32

C0.3

C1

(3.2

)5

258

18.2

32˚

50.3

ø33.5

ø23.8ø27.8ø31.5

80˚

C0.5

C1

0.13

(Oil)

ø27

1.5

45˚

ø23

100X /1.49oil

23˚CCG 0.19 0.17 0.13

HP Apo TIRF

∞/0.13-0.20 WD 0.12

M25×0.75

60.0

6

ø35.5

0.17

(CG

)

ø9ø29ø32

C0.3

C1

(3.2

)5

258

18.2

32˚

50.3

ø33.5

ø23.8ø27.8ø31.5

80˚

C0.5

C1

0.13

(Oil)

100X /1.49oil

23˚CCG 0.19 0.17 0.13

SR Apo TIRF

∞/0.13-0.20 WD 0.12

CFI HP Apochromat TIRF 100x Oil

CFI SR Apochromat TIRF 100x Oil

CFI SR Series

CFI HP Series

The SR (super resolution) objectives have been designed to provide superb optical performance with Nikon’s super-resolution microscope N-SIM. The adjustment and inspection of lenses using wavefront aberration measurement have been applied to yield optical performances with the lowest possible asymmetric aberration.

The CFI HP (High Power) objectives are optimized for the super resolution microscope N-STORM, which uses high power lasers to blink fluorophores at ultra-high speed. Thanks to the improved correction of axial chromatic aberration, these lenses enable high precision 3D multi-color fluorescence imaging.

M25×0.75ø23.8

ø6.9

ø28

25˚

55.5

5

ø22

0.17

(CG

)0.

13(W

D)

60.0

6

45

ø35

C2

31.6

ø27.5ø30

0.5

C1

(59.

74) 100X /1.40oil

HP Plan Apo VC

∞/0.17 DIC N2

JAPAN

CFI HP Plan Apochromat VC 100x Oil

Code.No Objectives NAW.D.(mm)

Physical depth of focus (µm)

Actual fieldof view (ømm)

Weight (g) Remarks

MRY10060 CFI SR Plan Apochromat IR 60x Wl 1.270.17

(0.18–0.16)0.23 0.37 250

Spring-loadedCover glass correction 0.15–0.19mm

MRY10059 CFI SR Apochromat TIRF 100x Oil 1.49 0.12 0.19 0.18 275 Cover glass correction0.13–0.19mm (23C˚)0.14–0.20mm (37C˚)

Code.No Objectives NA W.D. (mm)Physical depth of

focus (µm)Actual field

of view (ømm)Weight (g) Remarks

MRD01902 CFI HP Plan Apochromat VC 100x Oil 1.40 0.13 0.21 0.25 300 Spring-loaded

MRD01993 CFI HP Apochromat TIRF 100x Oil 1.49 0.12 0.19 0.18 300Cover glass correction0.13–0.19mm (23C˚)0.14–0.20mm (37C˚)

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CFI Apochromat λS SeriesThe λS series objectives provide chromatic aberration correction over a wide wavelength range from 405nm, and are powerful enough for spectral imaging and simultaneous multi-wavelength acquisition. The LWD 20x/40x WI λS lens has an extremely wide chromatic aberration correction range of 405nm to 950nm and is suitable for multiphoton observation. The 40x WI λS lens has an NA of 1.25, the world’s highest for a 40x water immersion objective. The 60x oil λS lens offers high level chromatic aberration correction across the whole visible range and is a powerful tool for confocal spectral imaging and photostimulation.

0.110.170.23

M25×0.75

ø31

ø35

ø37

ø34.6ø29.5ø24.3

ø9

60.0

6

5

6.5

19.5

7

80˚35˚

1

5.4

11.5

8

58.9

Apo LWD

∞/0.11-0.2320X/0.95 WI λS

WD 0.95

CFI Apochromat LWD 20x WI λS

M25×0.75

0.17

(CG

)

60.0

6

0.18

(wat

er)

21.5

616

.5

3.5

9.5

(2.9

04)

5

ø35.5

ø37.5

ø9ø19.3ø24.8ø33.5

11˚

22˚

45˚

11.8

Apo 40X /1.25 λS

0.19 0.17 0.15

∞/0.15-0.19 WD0.20-0.16

CFI Apochromat 40x WI λS

M25×0.75

Front focal point

Back focal point Pupil

position

ø9ø25.3

ø30

ø29ø31.5ø33.5

30˚

0.5

517

7.5

ø26.5

(5.2

9)

45˚

60.0

6

0.17

(CG

)0.

6(w

ater

)

45˚

245.

5

25.4

29.6

20.4

5

Apo 40Apo LWD

X /1.15 λS

0.19 0.17 0.15

∞/0.15-0.19 DIC NS

CFI Apochromat LWD 40x WI λS

M25×0.75

0.17

(CG

)

60.0

6

0.14

(Oil)

ø32

ø8

ø21.5

ø26

ø28.5

ø30.5

ø23.8

30˚

(59.

74)

5

45˚

45˚

3610

.25

4.25

ø27

55.0

8

60X /1.40 oil λS

Apo

∞/0.17 WD 0.14

CFI Apochromat 60x Oil λS

CFI60 Objectives

Code.No Objectives NAW.D.(mm)

Physical depth of focus (µm)

Actual fieldof view (ømm)

Weight (g) Remarks

MRD77200 CFI Apochromat LWD20x WI λS 0.95 0.95 0.41 1.1 245 Cover glass correction 0.11–0.23mm

MRD77400 CFI Apochromat 40x WI λS 1.250.18

(0.20–0.16)0.23 0.55 260

Spring-loadedCover glass correction 0.15–0.19mm

MRD77410 CFI Apochromat LWD40x WI λS 1.15 0.6 0.28 0.55 205 Spring-loadedCover glass correction 0.15–0.19mm

MRD71600 CFI Apochromat 60x Oil λS 1.40 0.14 0.21 0.367 180 Spring-loaded

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5.3

8.2

1.2

1238

.564

ø32

ø8

ø19

ø24

ø30

M25×0.75

10X / 0.50∞/ WD 1.20.17

S Fluor

M25×0.750.

17(C

G)

15.5

(WD

)

60.0

6(44.

39)

5

ø23.8

ø30

ø19.7

35˚

4X /0.20

S Fluor

∞/- WD 15.5

CFI S Fluor 10xCFI S Fluor 4x

1

5.3

5.9

1241

64.2

ø9

ø22ø25

ø33.5

ø30

M25×0.75

20X / 0.75∞/ WD 1.00.17

S Fluor

CFI S Fluor 20x

527

8.5

3.1

10.5

10.5

ø7.5

ø18

ø26

ø33

ø34.5

ø33

ø30

M25×0.75

11 14 17 20 23

40X / 0.9/ WD 0.30.11-0.23

Fluor

CFI S Fluor 40x

512

376.

54.

2

ø32

ø30

ø8.3

ø20

ø23.8

M25×0.75

40X / 1.30∞/ WD 0.220.17

S Fluor

Oil

CFI S Fluor 40x Oil

532

7.5

7.5

9.8

2.9

64.7

ø32

ø30

ø6

ø17

ø24

ø29.5

M25×0.75

1.3 0.7

100 X /0.5-1.3 oilS Fluor

∞/ WD 0.20.17

CFI S Fluor 100x Oil

CFI S Fluor SeriesThis CFI S Fluor series ensures a high transmission rate of ultraviolet wavelengths down to 340nm for fluorochromes like indo-1 and fura-2. Also, these objectives have improved S/N ratios for short wavelengths and have high NA, making the fluorescence images they produce significantly sharper and brighter.

Code.No Objectives NAW.D.(mm)

Physical depth of focus (µm)

Actual fieldof view (ømm)

Weight (g) Remarks

MRF00040 CFI S Fluor 4x 0.20 15.5 6.88 5.5 115

MRF00100 CFI S Fluor 10x 0.50 1.2 1.1 2.2 275 Spring-loaded

MRF00200 CFI S Fluor 20x 0.75 1.0 0.49 1.1 275 Spring-loaded

MRF00400 CFI S Fluor 40x 0.90 0.3 0.34 0.55 285Spring-loadedCover glass correction 0.11–0.23mm

MRF01400 CFI S Fluor 40x Oil 1.30 0.22 0.25 0.55 275 Spring-loaded,With stopper

MRF02900 CFI S Fluor 100x Oil with iris diaphragm 0.50-1.30 0.2 0.25 0.22 285 Spring-loaded

Page 20: Microscope Components 2CE-MUZH-4 - · PDF fileS Plan Fluor DIC N1 2 1.2 0.17 0 Microscope Components for Fluorescence Illumination and Transmitted Light Applications Microscope Components

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• Long WD and high NA at any magnification.• Sharper tips and broad approach angles provide improved accessibility for manipulator control.• Aberrations are corrected even in the infra-red range with the high-magnification objectives, making them

suitable for multi-photon imaging using infra-red light.• 100xW objective with a correction ring that corrects spherical aberration induced by imaging depth or

temperature fluctuations. With excellent infra-red transmission, this lens assures best quality images of even a thick specimen.

• The 16x objective is ideal for patch-clamp experiments when combined with the FN1 microscope and magnification module. With its excellent IR transmission and high NA, this lens is suitable for IR-DIC observation and provides superb image quality in combination with confocal microscopes.

• The 25xW MP objectives provide the high numerical aperture of 1.10 and a long 2.0 mm working distance, and are ideal for deep multiphoton imaging with a collar that corrects spherical aberrations depending on the depth of the specimen. Chromatic aberrations are effectively corrected from ultraviolet to infrared wavelengths, and transmission is increased by applying a Nano Crystal Coat.

M25×0.75

1.5

5

ø23.8

ø28

43

55.9

(56.

9)

5

60˚

ø5.5

ø6.5

ø18.5

ø21.5

ø24

60˚

C0.5

45˚

60.5

3.5(

wat

er)

10X /0.3W

Plan Fluor

DIC N1

∞/0 WD 3.5

JAPAN

CFI Plan Fluor 10x W

Lens

M25×0.75

ø6

ø17.8

ø22.8

ø25

44.2

50

57.2

5

5.9

C1.2

C0.5

2(W

D)

60.5

5(1

.25)

C0.75

35.2

32.9

ø29.5

ø23.8

ø5

20FluorX /0.50W

DIC M∞/ WD 2.00

CFI Fluor 20x W

M25×0.75

(58.

5)

55

(4.5

)

ø4

ø19ø23

ø25.5

ø29

45˚

60.5

2(w

ater

)

5

45˚

50˚

32.7

11.3

ø11.7

ø23.8

9.55

ø12.5

40FluorX /0.80W

DIC M∞/ WD 2.00

CFI Fluor 40x W

Water Dipping SeriesCFI60/CFI75 Objectives

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HL

M25×0.75

Index

Lens

60.5

ø35.5

ø28

ø27.5

5

1 6.5

ø35

ø22.8

ø10.8

C180˚

20˚30˚

ø8.3

ø11.6

0.6

4.2

45˚

13

5

C0.3

C0.5

C0.5

ø34

16.1

ø32.5

17.9

9.1

2.5

2.6(

wat

er)

Plan 100X /1.10W∞/0 WD 2.5 DIC H/N2

M25×

0.75 ø29

v5.5

ø19.2ø23

ø25.5

85.

5(1

)

(58.

5)5

60.5

2(W

D)

40˚

30˚

32.7

11.3

ø23.8

9.55

ø9

60FluorX /1.00W

DIC H∞/ WD 2.00

CFI Plan 100x W

CFI Fluor 60x W

M32×0.75ø30.8ø35

45˚

2.5

457

.566

.8(7

2)5

C1

45˚

45˚

ø6ø15ø16ø24ø29

3(w

ater

)7516 X /0.80W

LWD

∞/0 WD 3.0DIC N2

M25×0.75ø23.8

ø28

45˚

51.

5

50

53.3

(57)

45˚

45˚

50˚

5

ø6

ø13.7

ø19

ø20.4

ø24

60.5

3.5(

wat

er)

40X /0.80W

NIR Apo

DIC N2∞/0 WD 3.5

JAPAN

CFI75 LWD 16xW

CFI Apochromat 40xW NIR

M32×0.75

Lens

59.

611

.66.

518

.318

.56.

05(2

.41)

ø38

ø30.8

ø36

ø34.1

ø29.4

2.04

3(W

D)

ø6.1

33˚

ø12.9

75

ø34.5

45˚

ø31.5

45˚

ø35.6

45˚

ø13.6

Apo LWD 25X /1.10W

CG

SAMPLE DEEP

0.17 0.12

∞/0-0.17 WD 2.0

M25×0.75ø23.8

ø28

45˚

1.5

5

52.8

57

(57.

6)5

C1

40˚

30˚

ø8.5

ø10.6

ø11.4

ø19.2

ø24

60.5

2.8(

wat

er)(0

.93)

(3.2

7)

60X /1.0W

NIR Apo

DIC N2∞/0 WD 2.8

JAPAN

CFI75 Apochromat 25xW MP CFI75 Apochromat 25xW MP 1300

CFI Apochromat 60xW NIR

Code.No Objectives NAW.D.(mm)

Physical depth of focus (µm)

Actual fieldof view (ømm)

Weight (g) Remarks

MRH07120 CFI Plan Fluor 10x W 0.30 3.5 4.07 2.5 110 No cover grass

MRF07220 CFI Fluor 20x W 0.50 2.0 1.47 1.25 130 No cover grass

MRF07420 CFI Fluor 40x W 0.80 2.0 0.57 0.625 210 No cover grass

MRF07620 CFI Fluor 60x W 1.00 2.0 0.37 0.367 210 No cover grass

MRD07420 CFI Apo 40xW NIR 0.80 3.5 0.57 0.55 120 No cover grass

MRD07620 CFI Apo 60xW NIR 1.00 2.8 0.37 0.367 125 No cover grass

MRL07920 CFI Plan 100x W 1.10 2.5 0.3 0.22 225With Correction ring 0-0.13mmNo cover grass

MRP07220 CFI75 LWD 16xW 0.80 3.0 0.57 1.375 195 No cover grass

MRD77220 CFI75 Apo 25xW MP 1.10 2.0 0.3 0.88 360With Correction ringCover glass correction 0–0.17mm

MRD77225 CFI75 Apo 25xW MP 1300 1.10 2.0 0.3 0.88 360With Correction ringCover glass correction 0–0.17mm

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Nikon has developed dedicated objectives for advanced modulation contrast. Colorless and transparent samples can be observed in high relief with a plastic dish, which is not possible in DIC observation. The direction of contrast can be matched to S Plan Fluor ELWD NAMC objectives, thereby allowing optimal contrast selection for techniques like microinjection and ICSI.

M25×0.75

ø13.5

ø25

ø27.8

ø27

ø23.8

(6.2

3)1.

2

60.4

15

2.48

ø16

45˚

27.5

518

10 X /0.25

NAMC1

∞/1.2 WD 6.2

M25×0.75

1.2(

CG

)7.

4(W

D)

60.4

1

55

2(3

.31)

ø15ø22.8ø24.5

ø32ø34ø37

ø35

ø27.5ø31

35˚45˚45˚

9.5

3.5

5.5 10

.5

83˚

5.9

6.6

ELWD20X/0.45NAMC2

S Plan FluorCLAMP

2 0.171.2

CFI NAMC 10x

CFI S Plan Fluor ELWD NAMC 20xC

M25×0.7560

.41

1.2(

CG

)3.

1(W

D)

ø8.5

ø14.6

ø16

ø26

ø27.8

ø27

4.5

185

(56.

11)

45˚45˚

29 LWD20X/0.40

NAMC2

∞/1.2 WD 3.1

M25×0.75

1.2(

CG

)

ø32ø34

5

9.3

6.7

52

60.4

1

ø39.5

30˚

ø11ø27.5ø30.5

ø37.5

45˚

3.1(

WD

)

8.7

5.5

49.

5

(56.

11)

ø33.5

82˚

ELWD40X/0.60NAMC3

S Plan Fluor

2 0.171.2

CLAMP

CFI LWD NAMC 20xF

CFI S Plan Fluor ELWD NAMC 40xC

M25×0.75

ø12

ø20

ø25.5

15

0.5

85˚

60.4

1

1.2(

CG

)2.

1(W

D)

4.5

0.4

ø28

ø29

ø32

80˚19

57

41.

6

(57.

11)

ø34

ø3140˚

ø26.5

45˚

LWD40X/0.55

NAMC3

2 1.6 1.2 0.8 0.4 0

∞/0-2 WD 2.7-1.7

M25×0.75

220.

7

6.5

5

ø28.5ø29.5ø34

ø35.5

ø19.8ø24.8ø27

ø32.5

ø14.5

0.9(

CG)

60.3

1

(58

.2)

16˚

45˚(8

.2)

26˚

1.2(

WD

)

ø23.8

522

.3LWD IMSI 100X/0.85

1.3 0.60.9

∞/0.6-1.3 WD 0.95

CFI LWD NAMC 40xC

CFI Plan LWD IMSI 100xC

Nikon Advanced Modulation Contrast (NAMC) SeriesCFI60 Objectives

Code.No Objectives NAW.D.(mm)

Physical depth of focus (µm)

Actual fieldof view (ømm)

Weight (g) Remarks

MRP60105 CFI NAMC 10x 0.25 6.2 4.4 2.2 165 Cover glass thickness 1.2mm

MRP66205 CFI LWD NAMC 20xF 0.40 3.1 1.72 1.1 180 Cover glass thickness 1.2mm

MRP65425 CFI LWD NAMC 40xC 0.55 2.1

(2.7–1.7)0.91 0.55 250 Cover glass correction 0–2mm

MRH68200 CFI S Plan Fluor ELWD NAMC 20xC 0.45 7.4 1.36 1.1 225 Cover glass correction 0–2mm

MRH68400 CFI S Plan Fluor ELWD NAMC 40xC 0.60 3.1 0.76 0.55 275 Cover glass correction 0–2mm

MRL65900 CFI Plan LWD IMSI 100xC 0.85 1.15

(1.3-0.95)0.38 0.25 260

Spring-loadedCover glass correction 0.6–1.3mm

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No Cover Glass Objective Lenses

M25×0.75

60

0.53

550

.53.

5(5

.5)

ø13.8

ø7.5

ø18.8

ø30

45˚

45˚

ø23.8

ø24

40 X /0.65NCG

Plan

∞/0 WD 0.48

CFI Plan Achromat NCG 40x

M25×0.75

60

1.08

547

.5

4.5

3.5

(3.4

2) ø8.8

ø18

ø21.8

ø24

ø30

ø23.8

100X /0.90NCG

Plan

∞/0 WD 1.0

CFI Plan Achromat NCG 100x

ø23.8

ø28ø22

11˚

0.17

4(O

il)

25˚

ø6.5

60

ø31

C1

45˚

ø27.5ø30ø35

M25×0.75

100X /1.40oilPlan Apo

∞/0 DIC N2

JAPAN

50.5

12.9

31.6

10.5

(4.326)

CFI Plan Apochromat NCG 100x Oil

Code.No Objectives (magnification) NAW.D.(mm)

Physical depth of focus (µm)

Actual fieldof view (ømm)

Weight (g) Remarks

MRL03401 CFI Plan Achromat NCG 40x 0.65 0.48 0.65 0.625 160 Spring-loaded No cover grass

MRL03901 CFI Plan Achromat NCG 100x 0.90 1.0 0.34 0.25 160 Spring-loaded No cover grass

MRD04901 CFI Plan Apochromat NCG 100x Oil 1.40 0.16 0.21 0.25 300 Spring-loaded No cover glass

Page 24: Microscope Components 2CE-MUZH-4 - · PDF fileS Plan Fluor DIC N1 2 1.2 0.17 0 Microscope Components for Fluorescence Illumination and Transmitted Light Applications Microscope Components

24

100X / 1.30 oil Plan Fluor

DIC H ∞/ 0.17 WD 0.20

28

22.5

5

ø36f7 -0.025-0.050

ø33

Appr

ox. 7

0~17

014

8

M38×0.5

Image plane

Objective's shoulder

CFI Tube Lens UnitThis unit is attached to infinity objectives when neither a binocular or trinocular eyepiece tube is used.• Infinity Tube Lens Unit for CFI objectives (chromatic

aberration-free infinity corrected objectives).• Focal length: 200mm (7.87 in.)• To obtain the optimal objective performance, keep the

distance between the tube lens and the objective’s shoulder within 70–170 mm (2.76–6.69 in.).

CFI60 Objectives

Glossary

Numerical aperture is generally indicated by the equation below.

Numerical aperture is the most important factor in judging the objective's resolving power, brightness,

and depth of focus.

Working Distance (W.D.) and Parfocal DistaneceWorking distance is the distance between the top lens of the objective and the surface of

the specimen (or the cover glass) when the specimen is focused. The distance between the

objective's shoulder and the specimen (or the cover glass) when the specimen is focused is

referred to as parfocal distance. Nikon's CF infinity objectives have a parfocal distance of 60mm.

Objective Lens

(n=1 for air, 1.515 for oil, 1.333 for water)

Numerical aperture (NA)

Where,NA = n × sin θ n = Refractivity of the substance existing between the specimen and the objective.

sinθ = Angle that is formed by the optical axis and the light ray that passes to the extreme periphery of the objective lens.

The closest proximity of two objects that can be seen as two distinct regions of the image. Resolving power

is generally indicated by the equatuon below, where the larger the NA the greater the resolving power.

Resolving Power

Resolving Power =λ

NAWhere,λ = Light source's wavelength (generally 0.55μm)NA = Numerical aperture of objective

Specimen

0.61 ×

Working Distance

Parfocal Distance

Immersion Oil

Cover Glass

CFI Second Objective Lens UnitMXA22018

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25

The range in front of and behind the target plane of the specimen, within which the observed structure can be sharply focused. The accommodation

power of the human eye varies from person to person, so does depth of focus. Depth of focus is indicated by the equation below.

Depth of Focus (When observing with eyepieces)

Depth of focus =

Physical depth of focus =

n × λ

n × λ

2 × (NA)2

2 × (NA)2

λ = Light source's wavelength (generally 0.55μm)NA = Numerical aperture of objectiveM = Total magnificationn = Refractivity of the substance existing between the

specimen and the objective. (n=1 for air, 1.515 for 0il, 1.333 for water)

+ n 7 × NA× M × 1000

The pupil diameter of the objective lens is expressed by the following equation:

Pupil Diameter

Focal length

Pupil diameter = 2 × f × NA

f =200/Mo Mo = Objective's magnification

f = Focal length of objective lensNA = Numerical aperture of objective

When viewed through eyepieces

Total Magnification

When viewed on monitors

Eyepiece observation magnification (M) = objective's magnification × eyepieces magnification

Monitor observation magnification = objective's magnification × TV adapter magnification × monitor magnificationMonitor magnification varies depending on the imaging device size of the TV camera used and the monitor size. For information, see the table below.

Imaging device size Monitor magnificationType Diagonal length Longer side Shorter side

1/3-inch 6.0mm 4.8mm 3.6mm

1/2-inch 8.0mm 6.4mm 4.8mm

2/3-inch 11.0mm 8.8mm 6.6mm

Monitor Size

Imaging device size 9-inch 14-inch 20-inch

1/3-inch 38.1× 59.2× 84.6×

1/2-inch 28.6× 44.4× 63.5×

2/3-inch 20.8× 32.3× 46.2×

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Condensers

ø55

ø59

ø43

ø40

ø54

R400.

78

54.9

55.6

0.40.3

0.20.1

0.50.6

0.7

0.8M

AD

E IN

CH

INA

Achr N

A=0.9

C-SW

R68

35.5

70

27.5

R69

47

98.8

31.8

54.8

7.7 ø40

70˚

C-C Abbe Condenser Dry

C-SW Achromat Swing-out Condenser 1-100x

ø58

40R

ø55

ø54

854

.52

56.5

ø36.8

ø40

R42

R45

0.8 0.6 0.5 0.2 0.10.4 0.3Achr N.A=0.9

0.7C-C

ø40

824

22.2

8.5

54.7

11.

2 ø27ø36ø66

70˚

C-AR Achromat Condenser

C-SA Slide Achromat Condenser

ø27

59

63

98

ø60

ø40

55

870

˚

ø8

30˚

0.20.8 0.6 0.40.90 JAPANAchr

Achr-Apl N.A=1.41.4 1.2 1.0 0.8 0.6 0.4 0.2

(Oil)

(Slid

glas

s th

ickn

ess)

ø40ø62

70˚

9

54.9

0.6

55.5

1ø54

C-SWA Achromat Swing-out Condenser 2x-100x

C-AA Achromat /Aplanat Condenser

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27

ø59

ø42

ø22

ø36.8

ø54.5

1.5

8.6

8

63.4

54.8

29.8

24.5

12

ø40

C-LAR LWD Achromat Condenser

ø39

ø40

ø56

ø34.5

55.5

550.

5(O

il)20

70˚

149.

5

81

Dark Field CondenserOil 1.43-1.20

JAPAN

(Slid

glas

s th

ickn

ess)

Darkfield Condenser Oil

C-C Phase Contrast Turret Condenser NI-CUD Universal Condenser Dry

ø35.5

ø40

ø56

55.6

54

70˚

20.6

18

Dark Field CondenserDry 0.95-0.80

JAPAN

(Slid

glas

s th

ickn

ess)

Darkfield Condenser Dry

ø30

11.5

14.9

C-CEL

C-CEL Expander Lens (for darkfield)

>A

BS

<

30.5

24.4

9

ø43

ø51

50

3363

ø113

67.8

ø169

33

ø45

ø4740

Code.No Condensers NA O.D (mm)* Magnification Weight (g)**

MBL71100 C-C Abbe Condenser, Dry Type 0.9 1.9 4–100x 90

MBL71205 C-AR Achromat Condenser 0.85 4.2 4–100x 150

MBL11300 C-SWA Achromat Swing-out Condenser (2x-100x) 0.9/0.22 1.8 2–100x 105

MBL71305 C-SW Swing-out Achromat Condenser 1x-100x 0.9/0.11 1.4 1–100x 500

MBL71505 C-SA Slide Achromat Condenser 0.9 2.2 2-100x 250

MBL71405 C-AA Achromat /Aplanat Condenser 1.4 1.6 10–100x 180

MBL16105 C-LAR LWD Achromat Condenser 0.65 10.2 4–40x 118

MBL12000 Dark Field Condenser, Oil 1.2–1.43 1.5 20–100x 163

MBL12010 Dark Field Condenser, Dry 0.8-0.95 4.0 20–40x 175

MBL73100 C-C Phase Contrast Turret Condenser 0.9 1.9 10–100x 370

MBL99005 NI-CUD Universal Condenser Dry 0.88 2.5 2–100x 800

*Object Distance **Approximately

Page 28: Microscope Components 2CE-MUZH-4 - · PDF fileS Plan Fluor DIC N1 2 1.2 0.17 0 Microscope Components for Fluorescence Illumination and Transmitted Light Applications Microscope Components

28

Eyepieces

22.5

CFI

10x/22

ø30

ø34

ø38.

8

49-57

CFI 10x

CFIU

W10

X/25

ø30

ø34

ø38.

8

22.5

48-56

CFI UW 10x

CFI 12.5

X / 16

22.5

42–47

ø30

ø35

ø38.

8

CFI 12.5x

22.5

37–41

ø30

ø35

ø38.

8

CFI 15

X / 14.5

CFI 15x

Code.No Eyepieces Field number Weight (g)*MAK10110 CFI 10x 22 75

MAK30105 CFI UW 10x 25 100

MAK10120 CFI 12.5x 16 63

MAK10150 CFI 15x 14.5 48

*Approximately

Page 29: Microscope Components 2CE-MUZH-4 - · PDF fileS Plan Fluor DIC N1 2 1.2 0.17 0 Microscope Components for Fluorescence Illumination and Transmitted Light Applications Microscope Components

29

Eyepiece Tubes64

79 80.9

226.7

60

100

55

C-TB Binocular Tube

(Min

. dis

tanc

e)(M

in. a

ngle

)

(Tiltin

g sec

tion)

114

142

825

82.8

51.9 85.6ø51.5

112

(0–40)

10˚

C-TE2 Ergonomic Binocular Tube

67.5

3

ø59

99.3

225

16.5

120

52

76.5

C-TF Trinocular Tube F

73.7

3

ø5999

.2

222.5

52

17.5

172

70

103.5

57.5

C-TT Trinocular Tube T

Code.No Eyepiece Tubes Field

number ObservationInclination

Interpupillaryadjustment

Intensitysplit ratio

Weight (g)*

MBB92106 C-TB Binocular Tube 22 25° 50 to 75mm 1400

MBB93800 C-TE2 Ergonomic Binocular Tube 22 10°-30° 50 to 75mm100:0 or 50:50

two-way switching2000

MBB93106 C-TF Trinocular Tube F 25 25° 50 to 75mm100:0 or 0:100

two-way switching2000

MBB93115 C-TT Trinocular Tube T 25 25° 50 to 75mm100:0, 20:80 or 0:100three-way switching

2500

*Approximately

Page 30: Microscope Components 2CE-MUZH-4 - · PDF fileS Plan Fluor DIC N1 2 1.2 0.17 0 Microscope Components for Fluorescence Illumination and Transmitted Light Applications Microscope Components

30

C-mount screw

ø25.4ø42

ø29.

1

11.3

40.3

99.8

46.5

464

21

ø30

17.5

26

71

62

130.8

Image plane

2112

5.8

ø60

11.340.3

151.

3

4.5 46

.5

99.8

ø29.

1ø3

0

10.5

ø54

130.8

62

Image plane

C-TEP2 DSC Port for Ergonomic Binocular TubeMBB96800

C-mount camera port with built-in 0.7x relay lens.

C-TEPF2.5 DSC Port F2.5x for Ergonomic Binocular Tube MBB96810

F-mount camera port with built-in 2.5x relay lens.

Camera Ports for Ergonomic Binocular Tube

Code.No Camera Ports for Ergonomic Binocular Tube Weight (g)*MBB96800 C-TEP2 DSC Port for Ergonomic Binocular Tube 600

MBB96810 C-TEPF2.5 DSC Port F2.5x for Ergonomic Binocular Tube 500

*Approximately

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31

Illumination Modules

NI-LH Precentered LamphouseMBE95205

C-HGFIB HG 100W Adapter RMBF72315

C-HGFI HG Fiber Illuminator “Intensilight”MBF72655

C-HGFIE HG Motorized Fiber Illuminator “Intensilight”MBF72665

*With the “Intensilight” C-HGFI HG Fiber Illuminator. (C-HGFIE is not equipped with a shutter opening/closing function and ND compensation dial.)

C-HGFIF 15 HG Fiber 1.5mMBF71610

C-HGFIF 30 HG Fiber 3.0mMBF71630

7695 110

32

133

115272142

2 4

17.8

130

8

110*90

258

278.

5

25.5

7

*1831

276

.513

7

ø36

ø52

ø61

83.5

ø15

ø17

ø7.5

ø5ø9

20 24

Code.No Illumination Systems Weight (g)*

MBE95205 NI-LH Precentered Lamphouse 1000

MBF72655 C-HGFI HG Fiber Illuminator “Intensilight” 6000

MBF72665 C-HGFIE HG Motorized Fiber Illuminator “Intensilight” 6000

MBF72315 C-HGFIB HG 100W Adapter R 450

MBF71610 C-HGFIF 15 HG Fiber 1.5m 100

MBF71630 C-HGFIF 30 HG Fiber 3.0m 150

*Approximately

Page 32: Microscope Components 2CE-MUZH-4 - · PDF fileS Plan Fluor DIC N1 2 1.2 0.17 0 Microscope Components for Fluorescence Illumination and Transmitted Light Applications Microscope Components

32

Intermediate Modules67

102

8610

4.5

R70.5

362

ø40

19 80 126.5

80

5

2542

137.

3

2

12

6

Light

axis

67

294128

172

140

23

R(86)

R(8

6)

7062

.7

120

445

24.5

206.5

8084

23

CI-FL Epi-Fluorescence AttachmentMBE94310

D-FL Epi-Fluorescence AttachmentMBE74100

100

13.5

4525

5

50.5

57.522

175

ø52

ø51

285.5(Top View)

(Right-side View)

5545

1000

Y-IDP Double PortMBB74100

Y-IDP Double Port 0/100MBB74105

Y-IM Magnification ModuleMBB75500

285.5

100

455 ø51

50.5 175

50

285.5

1.6

76.523.3

Page 33: Microscope Components 2CE-MUZH-4 - · PDF fileS Plan Fluor DIC N1 2 1.2 0.17 0 Microscope Components for Fluorescence Illumination and Transmitted Light Applications Microscope Components

33

210

191

15

58.538

3.5

ø42

11.516

15

ø51.5

77.5

5.5

66

ø42

3.5210

191

38 58.515

5.5

66

11.5

15

16

77.5

ø51.5

NI-FLT6-E Motorized Epi-fluorescence Cube Turret MBE94300

NI-FLT6 Epi-fluorescence Cube Turret MBE94500

NI-FLT6-I Intelligent Epi-fluorescence Cube Turret MBE94400

58.538

1519

1

210 3.5

ø42

77.5

ø51.5

5.5

66

21

38

60˚

35

3316.5

6.7

(24.7)

ø29.

5

16 18

30˚

C-FL BLK Blank Filter CubeMXA22030

Same as outline images of C-FL Epi-fl Filter Cubes.

Page 34: Microscope Components 2CE-MUZH-4 - · PDF fileS Plan Fluor DIC N1 2 1.2 0.17 0 Microscope Components for Fluorescence Illumination and Transmitted Light Applications Microscope Components

34

Code.No Intermediate Tubes Weight (g)*MBE94310 CI-FL Epi-Fluorescence Attachment 2000

MBE74100 D-FL Epi-Fluorescence Attachment 3300

MBB74100 Y-IDP Double Port 1300

MBB74100 Y-IDP Double Port 0/100 1300

MBB75500 Y-IM Magnification ModuleY-IDP Double Port 1300

MBE94300 NI-FLT6-E Motorized Epi-fluorescence Cube Turret 2500

MBE94400 NI-FLT6-I Intelligent Epi-fluorescence Cube Turret 2500

MBE94500 NI-FLT6 Epi-fluorescence Cube Turret 2500

MBE94100 NI-FLEI Epi-fluorescence Attachment 1500

MBE94200 NI-BPU Back Port Unit 2500

*Approximately

NI-BPU Back Port UnitMBE94200

A

A

125 128

ø12

ø10

12

ø32

ø9

ø75

199

15

5866

6625

ø8

3

14

35

ø10

2.5

77

70

1749

4941

7010

1

80

70

80

54.8

107.

7

4-ø11

66 2.5

NI-FLEI Epi-fluorescence AttachmentMBE94100

Intermediate Modules

Page 35: Microscope Components 2CE-MUZH-4 - · PDF fileS Plan Fluor DIC N1 2 1.2 0.17 0 Microscope Components for Fluorescence Illumination and Transmitted Light Applications Microscope Components

35

Contact Arm

NIU-CAM Contact ArmMBD92110

NI-SAM Standard ArmMBD92000

The MBD92110 NIU-CAM Contact Arm for use with Motorized Nosepieces and Intelligent Nosepieces on page 37, and the MBD92000 NI-SAM Standard Arm for use with Manual Nosepieces on the same pages.

(100

0)

(100

0)R33

.5

R80

127

53.5

319.

4

30

ø72

26

127 30

ø72

32

27

R86

.5

53.5

319.

4

107

R33.5

26

R48.5R41.5

49.2 69

.2

Code.No Contact Arm Weight (g)*MBD92110 NIU-CAM Contact Arm 1500

MBD92000 NI-SAM Standard Arm 1400

*Approximately

Page 36: Microscope Components 2CE-MUZH-4 - · PDF fileS Plan Fluor DIC N1 2 1.2 0.17 0 Microscope Components for Fluorescence Illumination and Transmitted Light Applications Microscope Components

36

Nosepieces

485153

ø104.8

105.450

22.5

C-N6 Sextuple Nosepiece ESDMBP71316

48

ø106

105.8

53

50

22.5

C-N6A Sextuple Nosepiece with Analyzer SlotMBP71325

48

ø116

22.5

117.850

53

D-ND6 DIC Sextuple NosepieceMBP73325

Page 37: Microscope Components 2CE-MUZH-4 - · PDF fileS Plan Fluor DIC N1 2 1.2 0.17 0 Microscope Components for Fluorescence Illumination and Transmitted Light Applications Microscope Components

37

48

ø116

ø43.5

80.7

50

22.5

117.8

53

NI-ND6-E Motorized DIC Sextuple NosepieceMBP98325

23

4

48

ø126.5

ø43.5

80.5

22.5

50

129

53

NI-N7-E Motorized Septuple NosepieceMBP98405

2

3

ø116

48

117.650

22.5

NI-ND6-I Intelligent DIC Sextuple NosepieceMBP99325

48

ø116

22.5

117.850

53

NI-N7-I Intelligent Septuple NosepieceMBP99405

Code.No Nosepiece Weight (g)*MBP71316 C-N6 Sextuple Nosepiece ESD 450

MBP71325 C-N6A Sextuple Nosepiece with Analyzer Slot 450

MBP73325 D-ND6 DIC Sextuple Nosepiece 1000

MBP98325 NI-ND6-E Motorized DIC Sextuple Nosepiece 1300

MBP98405 NI-N7-E Motorized Septuple Nosepiece 1300

MBP99325 NI-ND6-I Intelligent DIC Sextuple Nosepiece 1200

MBP99405 NI-N7-I Intelligen Septuple Nosepiece 1300

*Approximately

Page 38: Microscope Components 2CE-MUZH-4 - · PDF fileS Plan Fluor DIC N1 2 1.2 0.17 0 Microscope Components for Fluorescence Illumination and Transmitted Light Applications Microscope Components

38

ø23.1

ø25.4

30.7

116.

8

27417

.526

ø53

ø42

Image plane

C-Mount TV Adapter 0.6xMQD42060

Camera Adapters3 9.3

ø12

7227

ø52

ø38

35

ø52

V-T Photo AdapterMAB53410

76.5

ø51

ø38

ø38

ø59

Y-TV TV TubeMBB73525

ø38 8.5

55

ø59

ø50

46.5

ø40

Y-TV 0.55x TV TubeMBB73550

C-mount screw

Image plane

ø38

ø50

41

11.4

717

.526

25.5

ø25.4

30 °

C-DA C-Mount AdapterMQD42005

ø25.4

ø50

ø40

413

.8

17.5

26

13

30.8

Image plane

C-mount screw

30.8

ø23.1ø42

84.8

4

17.5

26

ø25.4ø32

Image plane

C-Mount Adapter 0.55xMQD42055

C-Mount TV Adapter 0.35xMQD51040

ø48ø25.4

ø38

25.5

31.0

8

4 1.58

17.5

26

Image plane

ø23.1

ø25.4

30.7

100.

1

27.3

417.5

26

ø53

ø42

Image plane

C-Mount Adapter 0.7xMQD42070

C-Mount TV Adapter 0.45xMQD42040

Page 39: Microscope Components 2CE-MUZH-4 - · PDF fileS Plan Fluor DIC N1 2 1.2 0.17 0 Microscope Components for Fluorescence Illumination and Transmitted Light Applications Microscope Components

39

ø38

68

4229

ø53

Image plane

C-Mount TV Adapter VM 2.5xMQD42120

ø38

109

83

ø53

29

Image plane

ø40 f8

ø17.3

ø60

1312

.546

.5

4.5

30

Image plane

ø43.2

ø40 f8

ø50

ø60

1373

.346

.5

4.5

10.5

90.8

Image plane

C-Mount TV Adapter VM 4xMQD42140

DS-F F-Mount Adapter MQD43000

DS-F2.5 F-Mount Adapter 2.5x MQD43020

Code.No CCTV Camera Adapters Weight (g)*MAB53410 V-T Photo Adapter 200

MBB73525 Y-TV TV Tube 350

MBB73550 Y-TV 0.55x TV Tube 300

MQD42005 C-DA C-Mount Adapter 180

MQD42055 C-Mount Adapter 0.55x 300

MQD42070 C-Mount Adapter 0.7x 155

MQD51040 C-Mount TV Adapter 0.35x 300

MQD42040 C-Mount TV Adapter 0.45x 620

MQD42060 C-Mount TV Adapter 0.6x 650

MQD42120 C-Mount TV Adapter VM 2.5x 165

MQD42140 C-Mount TV Adapter VM 4x 338

MQD43000 DS-F Mount Adapter 80

MQD43020 DS-F2.5 F-Mount Adapter 2.5x 200

*Approximately

Page 40: Microscope Components 2CE-MUZH-4 - · PDF fileS Plan Fluor DIC N1 2 1.2 0.17 0 Microscope Components for Fluorescence Illumination and Transmitted Light Applications Microscope Components

40

Main Body and Accessories

148.5

320

140

315

4

R800 R800R1500

815

315

34

ø48

143

R450

R1400

68

133

269

383

341.7

POWER

CAPTURE F.S.

ND

OUT

IN

8NCB11

ND32

Ni-U Main BodyMBA92010

(-1.

0 ~

36.

0)

2547 53

.5

22 13.6 147.8

11.3

23.5

459

76

R38

ø10

84 151

277.771.5

ø26.2

NI-CH Condenser HolderMBD94000

72.5

196

94

84120

20.5

91.5

7.5

NI-SS SubstageMBD93000

To mount the condensers in this brochure on the Ni-U main body, the following holder and substage are required.

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41

220

93

160

6

NI-CTLB Control Box BMBF95410

EPI FL INTSL

SHUTTER

12481632

INTSL ND

OBJ FL

CONTROL

NI-SRCPMADE IN JAPAN

DIA

OPEN / CLOSE

120.

5

2.2 20 76

1750

37

3010

NI-SRCP Simple Remote Control PadMBF95120

The control box and control pad below are required to use the Ni motorized unit and intelligent unit via the NIU-CAM Contact Arm (page 35).

Code.No Illumination Systems Weight (g)*MBD94000 NI-CH Condenser Holder 350

MBD93000 NI-SS Substage 800

MBA92010 NI-U Main body 11000

MBF95410 NI-CTLB Control Box B 2500

MBF95120 NI-SRCP Simple Remote Control Pad 200

*Approximately

Page 42: Microscope Components 2CE-MUZH-4 - · PDF fileS Plan Fluor DIC N1 2 1.2 0.17 0 Microscope Components for Fluorescence Illumination and Transmitted Light Applications Microscope Components

En(1609)T Code No.2CE-MUZH-4 This brochure is printed on recycled paper made from 40% used material.

Specifications and equipment are subject to change without any notice or obligation on the part of the manufacturer. September 2016  ©1997-16 NIKON CORPORATION

N.B. Export of the products* in this brochure is controlled under the Japanese Foreign Exchange and Foreign Trade Law. Appropriate export procedure shall be required in case of export from Japan.*Products: Hardware and its technical information (including software)

NIKON CORPORATIONShinagawa Intercity Tower C, 2-15-3, Konan, Minato-ku, Tokyo 108-6290, Japan phone: +81-3-6433-3705 fax: +81-3-6433-3785http://www.nikon.com/products/microscope-solutions/

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WARNINGTO ENSURE CORRECT USAGE, READ THE CORRESPONDING MANUALS CAREFULLY BEFORE USING YOUR EQUIPMENT.

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