P6693Epidemiologic analysis of eyelash characteristics with increasing age in apopulation of healthy women
Dee Anna Glaser, MD, Department of Dermatology, Saint Louis University, SaintLouis, MO, United States; Carrie Caulkins, PhD, Allergan, Inc, Irvine, CA, UnitedStates; Derek Jones, MD, Division of Dermatology, David Geffen School ofMedicine, University of California at Los Angeles, Los Angeles, CA, United States;Jean Carruthers, MD, Department of Ophthalmology and Visual Sciences,University of British Columbia, Vancouver, Canada; Joan Largent, MPH, PhD,Allergan, Inc, Irvine, CA, United States
Background: As women age, eyelashes tend to get thinner, shorter, and lighter. Thisphenomenon may be caused by various factors, including a shortening of thegrowth phase and hormonal changes. To date, no empirical data have been reportedto quantify this phenomenon. Therefore, the current study was undertaken toinvestigate how eyelash characteristics (ie, length, thickness, and darkness) corre-late with advancing age.
Methods: This was a cross-sectional observational study of healthy adult femaleswith natural eyelashes. Qualified subjects had their demographic informationrecorded and their eyelashes photographed and analyzed using digital imageanalysis (DIA) during a single encounter. Upper eyelash length (mm), thickness(mm2), and darkness (intensity, ranging from black ¼ 0 to white ¼ 255) wascalculated based on DIA of superior-view eyelash photographs. Data from both theleft and right eye was averaged for each subject for statistical analysis. Linearregression was used to assess the association between age (years, continuous) andmeanmaximum eyelash length, mean eyelash thickness, andmean eyelash intensity.Race (white and nonwhite) was also tested as a potential confounding variable andincluded in the linear regression models.
Results: A total of 179 subjects aged 22 to 65 years were enrolled with a mean age of40.36 10.3 years. Subjects werewhite (46.1%), Asian (36.5%), Hispanic (9.0%), EastIndian (5.1%), and African American (3.4%). Mean maximum eyelash length rangedfrom 6.39 6 1.02 mm in subjects aged 50-65 years to 7.98 6 1.15 mm in subjectsaged 22 to 29 years. Mean eyelash thickness ranged from 1.17 6 0.42 mm2 insubjects aged 50 to 65 years to 1.62 6.0.56 mm2 in subjects aged 22 to 29 years.Mean eyelash intensity ranged from 118.2 6 19.8 in subjects aged 30 to 39 years to129.46 17.3 in subjects aged 50 to 65 years. Eyelash length and thickness decreasedwith increasing age (P\.0001; P\.0001, respectively), and intensity (lightness)increased with increasing age (P\.05) while adjusting for race.
Conclusion: Advancing age among an ethnically diverse population of healthywomen is associated with significant decreases in eyelash length, thickness, anddarkness.
APRIL 20
ponsored by Allergan.
100% is sP6569Evaluation of the role of dermatopontin in skin aging by changing skinviscoelastic properties: In vivo study
Yolene Guerif Ferreira, Ashland, Sophia Antipolis, France; Arlette Berghi,Ashland, Sophia Antipolis, France; Cl�emence Metro, Ashland, Sophia Antipolis,France; Corinne Coquet-Morel, Phd, Ashland, Sophia Antipolis, France; GillesOberto, Ashland, Sophia Antipolis, France; Karine Cucumel, Phd, Ashland,Sophia Antipolis, France; Nouha Domloge, MD, Ashland, Sophia Antipolis, France
The loss of elasticity is the most striking response to skin aging, leading to theappearance of wrinkles and skin slackening. Recent studies have demonstrated thekey role of the protein dermatopontin (DPT) in matrix assembly. It acceleratescollagen fibrillogenesis, affects the diameters of newly formed collagen fibrils, and asa consequence may play a critical role in skin elasticity. In order to evaluate the roleof extracellular matrix, and especially DPT in dermal aging, a new compoundIV09.021 was designed to modulate positively DPT expression and enhanced theexpression of ECM proteins (in vitro and ex vivo studies). In this study, we wereinterested in evaluating the role of IV09.021 in modulating viscoelasticity propertiesof the skin in vivo. A 28-day in vivo double-blind studywas conducted with IV09.021compound at 1% versus placebo on the neckline of 12 volunteers. The volunteersapplied the inducer-containing cream and the placebo cream twice a day during thetest. The mechanical properties of the skin were assayed with Reviscometer RVM600 and the viscoelasticity properties of the skin was appreciated regarding the RRTangular profile, the anisotropy (RRTmax/RRTmin) and the Langer’s line width,which all give an idea of the dependence of the mechanical properties to thedirection of the collagen fibers. After 4 weeks of IV09.021 treatment, the RRTangular profile got closer to a RRT angular profile of a young skin, the anisotropydeclined and the Langer’s line width increased. These results showed that IV09.021restores the young viscoelasticity property of the skin. Interestingly, Visioscanpictures and the clinical examination revealed that skin microrelief were lessmarked and led to triangular and hexagonal skin image as expected in young skin.These in vivo results confirm that IV09.021 compound leads to an improvement ofthe ‘‘quality’’ of ECM matrix, decreasing the visual feature of aging.
cial support: None identified.
Commer13
P6656Evidence that an olive derivative can beneficially affect intrinsic aging,photoaging, and inflammatory skin conditions based on gene expressionprofiling and pattern matching
Robert L. Binder, PhD, Procter and Gamble Beauty, Cincinnati, OH, UnitedStates; Jun Xu, PhD, Procter and Gamble Beauty, Cincinnati, OH, United States;Michael K. Robinson, PhD, Procter and Gamble Beauty, Cincinnati, OH, UnitedStates; Raghu Kainkaryam, PhD, Procter and Gamble Beauty, Cincinnati, OH,United States; Rosemarie Osborne, PhD, Procter and Gamble Beauty, Cincinnati,OH, United States; Scott M Hartman, MS, Procter and Gamble Beauty,Cincinnati, OH, United States
Background: Global gene expression profiles can serve as indicators of phenotypes,and coupled with pattern matching can be used to link diseases to beneficialmaterials. Two of the authors (R.K. and J.X.) have developed a novel patternmatching algorithm called FaceMap that is based on methods used for facialrecognition. We applied FaceMap to link gene expression profiles induced bychemicals in cultured cells to skin aging and atopic dermatitis.
Objective: To use gene expression profiling and FaceMap to investigate the activitiesof an olive derivative (olive oilederived fatty acids modified with PEG-7).
Methods: Human telomerized keratinocytes and BJ fibroblasts were treated with theolive derivative for 6 or 24 hours. RNA was isolated and gene expression profilingwas conducted by standard methods using Affymetrix GeneChips. The olivederivative data were added to a large set of expression profiles induced by otherchemicals in similar experiments and compared using FaceMap analysis to clinicalgenomics data. Skin aging data were from a clinical study we previously conductedin which full thickness biopsies of sun-exposed (outer forearm) and sun-protected(buttock) skin from young (age 18-20) and older (age 60-67) female subjects weresubjected to gene expression analysis on Affymetrix GeneChips. We also obtainedpublished clinical transcriptomics data on atopic dermatitis from the GeneExpression Omnibus database.
Results: FaceMap analysis yields a distance measure for each of the materials testedcompared to the respective conditions. Materials that tend to mimic the conditionwill have positive scores and materials that tend to reverse the pattern of geneexpression in the condition (predicted to be beneficial) will have negative scores. Inthe skin aging study, the comparison between the older to younger arms is anindication of photoaging and the buttock comparison is indicative of intrinsic aging.The olive derivative tested in both cell lines linked negatively to the gene expressionpatterns in photoaging and intrinsic aging. Also, the olive derivative tested inkeratinocytes linked negatively to atopic dermatitis. An extremely potent irritantreference material, cantharidin, linked strongly positively to atopic dermatitis,supporting the validity of the olive derivative results. Overall, these data support thatthe olive derivativewill have beneficial effects in both aging skin and skin affected byinflammatory conditions.
ponsored by Procter and Gamble.
100% is sP6642Expression profiles of cholesterol biosynthetic pathway associated withskin intrinsic and extrinsic aging
Bradley Jarrold, MS, Procter and Gamble Beauty, Cincinnati, OH, United States;Jay Tiesman, PhD, Procter and Gamble Beauty, Cincinnati, OH, UnitedStates; Makio Tamura, PhD, Procter and Gamble Beauty, Cincinnati, OH,United States; Michael Robinson, PhD, Procter and Gamble Beauty, Cincinnati,OH, United States; Robert Binder, Procter and Gamble Beauty, Cincinnati, OH,United States; Rosemarie Osborne, PhD, Procter and Gamble Beauty, Cincinnati,OH, United States
Skin aging is a cumulative process resulting from unrepaired damage and age-relatedphysiologic changes caused by intrinsic (eg, free radicals) and extrinsic (eg, UVexposure) factors. In the current study, we examined the effects of intrinsic andphotoaging on stratum corneum (SC) cholesterol biosynthetic pathway of humanskin barrier in vivo. Furthermore, human skin cell cultures were utilized to examinehow cosmetic compounds, known to improve skin barrier function, affect thesesame biomarkers in vitro. mRNA was purified from: (1) full thickness skin biopsiesfrom individual study subjects’ [young (18-20 yrs of age) or older (60-67 yrs of age)females] buttocks (sun-protected) and outer forearm (sun-exposed); and (2) humanskin cells treated in vitro with Pal-KTTKS or a olive oilederivative for 6 hours.Labeled target cRNA was hybridized to Affymetrix microarrays; bioinformaticsfocused on cholesterol metabolism genes. In both intrinsically and photoaged skin,there was a down-regulation in expression of genes involved in cholesterolbiosynthesis. In in vitro skin cells, the olive oilederivative and Pal-KTTKS treatmentsincreased the expression of cholesterol synthesis pathway genes in a directionsopposite to the effects of intrinsic and photoaging, suggesting improvement in SCbarrier function. The coordinated down-regulation of SC lipid pathways at the levelof gene expression is consistent with previously reported global decreases incholesterol in aging skin, and likely contributes to the decreased capacity of agedskin to maintain and repair the epidermal barrier. The olive oilederivative and Pal-KTTKS induced coordinate up-regulation in expression of cholesterol biosyntheticgenes, suggesting increased levels of this key lipid are available for SC maintenanceand repair.
d by Procter and Gamble Beauty.
SupporteJ AM ACAD DERMATOL AB29
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