viii

DAFTAR ISI

HalamanHALAMAN JUDUL ............................................................................................ i

HALAMAN SAMPUL............................................................................................ii

PENGESAHAN SKRIPSI ..................................................................................iii

PERNYATAAN ............................................................................................... iiiv

HALAMAN PERSEMBAHAN ........................................................................... v

KATA PENGANTAR ........................................................................................ vi

DAFTAR ISI……………………………………………………………….…….viii

DAFTAR GAMBAR ........................................................................................... x

DAFTAR TABEL............................................................................................... xi

DAFTAR LAMPIRAN......................................................................................xii

DAFTAR SINGKATAN...................................................................................xiii

INTISARI……….. ........................................................................................... xiv

BAB I. PENDAHULUAN ................................................................................... 1

A. Latar Belakang ......................................................................................... 1

B. Rumusan Masalah .................................................................................... 3

C. Tujuan Penelitian...................................................................................... 3

D. Tinjauan Pustaka ...................................................................................... 4

1. Kunyit (Curcuma domestica Val). .......................................................... 4

2. Tikus Galur Wistar. ................................................................................ 8

3. Madecassol® ......................................................................................... 10

4. Ekstraksi .............................................................................................. 11

5. Penyembuhan Luka .............................................................................. 15

6. Faktor-faktor yang Mempengaruhi Penyembuhan Luka........................ 23

E. Landasan Teori ....................................................................................... 24

F. Hipotesis ................................................................................................ 25

BAB II. CARA PENELITIAN........................................................................... 26

A. Rancangan Penelitian.............................................................................. 26

B. Definisi Operasional ............................................................................... 26

C. Alat dan Bahan yang Digunakan............................................................. 26

ix

D. Prosedur Penelitian ................................................................................. 29

1. Preparasi sampel .................................................................................. 29

2. Formulasi ............................................................................................. 30

3. Kromatografi Lapis Tipis ..................................................................... 32

4. Pengelompokan Hewan Uji dan Uji Aktivitas Penyembuhan Luka ....... 34

5. Pembuatan Preparat Histopatologi ........................................................ 36

6. Pengamatan Makroskopis dan Mikroskopis .......................................... 37

E. Cara Analisis .......................................................................................... 40

F. Skema Penelitian .................................................................................... 41

G. Perlakuan Hewan Uji.............................................................................. 43

BAB III. HASIL PENELITIAN DAN PEMBAHASAN .................................... 44

A. Ekstraksi Rimpang Kunyit (Curcuma domestica Val) ............................. 44

B. Perlakuan Hewan Uji.............................................................................. 44

C. Penutupan Luka...................................................................................... 46

D. Hemoragi................................................................................................ 48

E. Sel Radang ............................................................................................. 50

F. Fibroblast ............................................................................................... 51

G. Nekrosis ................................................................................................. 53

H. Diskusi Umum........................................................................................ 54

BAB IV. KESIMPULAN DAN SARAN ........................................................... 59

DAFTAR PUSTAKA ........................................................................................ 60

LAMPIRAN. ..................................................................................................... 64