Download pdf - Biology Xii project

7/24/2019 Biology Xii project

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Biology project

TO ESTIMATERELATIVE

AMOUNT OF

CAFFEINE INVARIOUS

BRANDS OF TEA


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CERTIFICATE


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1. Aim

2. Requirement

3. Introduction

4. Classifcation o tea plant5. Importance o tea

6. Extraction o ater solu!lecomponent rom tea sample"I#

$. Remo%al o tannic acid rom fltrate"II#

&. Extraction o ca'eine rom tannicacid ree fltrate"III#

(. Calculation

1). *!ser%ation ta!le

11. Experimental results12. +recautions and expected errors

13. !i!lio,rap-

CONTENT


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Extraction anddetermination o

ca'eine content in%arious tea samples

AIM


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/ea0er Conical as0 unnel eatin, arran,ement amples o tea Calcium car!onate

ilute sulp-uric acid c-loroorm

REQUIREMENT


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ost popular drin0s in Indiaare Tea a! Co"ee#As is t-epopularit o t-ese drin0s so is

t-e contri!ution o India in t-eirproduction. India -as !een t-eleadin, producer o tea.

8ea ,ros indeed9 ertile : ell

drained soil. It require arm andmoist climate t-rou,-out t-eear. requent s-oers9 elldistri!uted o%er t-e ear

ensures continuous ,rot- otender lea%es.

INTRODUCTION


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In India it ,ros mainl in lo-ills o Assam9 ills o ar;eelin,and est

/en,al and t-e -ill o =il,irietc. 8ea also causes man-armul disease suc- asia!etes9 pro!lems in ner%ous

sstem9 decrease in A=secretion -ic- causes increasein ine secretion and it -as=icotine -ic- is a timulant.


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?in,dom @ +lantae

Class @ icotledonae

*rder @ 8-eales

amil 8-eaceae

Benus @ 8-ea

pecies @ inensis

Cla$$i%catio o& tea

plat


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It is used as stimulant It -as anti%iral and ,ermicidal

acti%it /lac0 tea is capa!le o reducin,

c-olesterol and tri,lcerides8ea is diuretic su!stances8ea drin0in, acilitates muscle

contraction 9 induced ! ner%estimulation.

IM'ORTANCE OF

TEA


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se di'erence in t-e solu!ilito ca'eine and tannic acid inater and c-loroorm to

separate t-em rom tea lea%es.Ca'eine is muc- more solu!lein c-loroorm t-an in ater. /uttannic acid is extensi%el

solu!le in ater as ell asc-loroorm.

t(eory


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8a0e a!out 1))ml o distilledater in 25)ml conical as0.

Add 5,m o tea and !oil it or 4

to 5 minute. 8-en cool it andflter.

Repeat t-is process a,ain it-1))ml o distilled ater and

residual tea lea%e.8-e fltrate in eac- case s-ould

!e collected. =o t-e et tea samples ma

!e dried in an o%er andei,-ted.

E)tractio o& *ater Sol+,leCo-poet$ &ro- Tea Sa-ple .

Step.I


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8-e fltrate o!tained in t-e frst step isno !oiled it- 2@3 ,m. calciumcar!onate to ,et tannic acid9 present

in tea extract9 precipitates. *n lea%in,it or ten minutes ppt. is o!tained. ilter t-e solution it-out distur!in,

t-e ppt careull. 8-e ppt s-ould !etranserred to flter paper onl ater all

t-e fltrate is remo%ed.8annic acid -as !een extracted in t-e

orm o calcium ternate -ic- ontreatment it- -droc-loric acid ,i%etannic acid.

8-e fltrate is no ree rom tannicacid and can !e used to extractca'eine.

Re-o0al o& Taic Aci! &ro- Filtrate .Step.II


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8a0e tannic acid fltrate o!tained instep 2"ii# and add 1)) ml oc-loroorm in it in a separatin, unnel

and allo it to stand or 1) minutes. A,ain t-e same procedure as done in"i#

ix it it- t-e pre%ious one. rom t-is c-loroorm extract9

c-loroorm ma !e distilled ore%aporated.

8-e pale ,reen residue o!tained iscrude ca'eine.

Repeat t-is experiment it- ot-er!rands o tea and record ouro!ser%ation in a ta!ular orm.

E)tractio o& Ca"eie &ro- TaicAci! Free Filtrate . Step.III


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Calc+latio$ o& 1ag( ,a2ri

>1D 6 ,m

>2D ).$ ,m

+ercenta,e o ca'eine D ).$ 6 x 1))

D 11.6F

Calc+latio$ o& Lipto yello1 la,el

>1D 6 ,m

>2D ).& ,m +ercenta,e o ca'eine D 13.3F

Calc+latio$*eig(t o& tea +$e! 3 *4g-5

*eig(t o& Ca"eie 3 *6g-5Calc+latio 7

'ercetage o& $ol+te ca"eie 3 *4 8 *6 ) 499


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Calc+latio$ o& Taj Ma(al

>1D 6 ,m

>2D ).$ ,m

+ercenta,e o ca'eine D 11.6F

Calc+latio$ o& Re! La,el

>1D 6 ,m

>2D ).$2 ,m

+ercenta,e o ca'eine D 12


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Sr:o5

Bra!a-e

*4 *6 ;age o&ca"eie

1 >a,-!a0ri

6,m ).$,m 11.6

2 Giptonellola!el

6,m ).&,m 13.3

3 8a; ma-al 6,m ).$,m 11.6

4 Red la!el 6,m ).$2,m 12.)

O,$er0atio ta,le


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8-e tea sample o Gipton ellola!el contain -i,-est amount oca'eine and -ence H@

Bi%es !etter taste t-an ot-ers. Is more -armul or our -ealt-.

E)peri-et Re$+lt$


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C-loroorm s-ould not !e smelt directlor a lon, time as it itsel is toxic.

8-e measurement o ei,-t o tealea%es and all extract s-ould !e precise.

8-e measurement o ei,-t o teasample and all extract s-ould !eprecise.

8-e ater used s-ould !e distilled. Apparatus s-ould !e clean and dr. =o ater s-ould !e added to an

extract in !eteen t-e experiment as itma ,et errors in calculation.

=o prolon,ed -eatin, s-ould !e done asater ma e%aporate in lar,e quantitalon, it- same %olatile solutes

'reca+tio$ a! E)pecte!Error$ 7


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BIBLIO