98 PATENT ABSTRACTS

Arthur Riggs, assigned to Genentech Inc

The Specification discloses: 1. Recombinant microbial cloning vehicles comprising hete- rologous DNA coding for the expression of mammalian hormone (e.g., somatostatin) and other poiypeptides, including plasmids suited for the transformation of bacterial hosts. The latter incorporate a regulon homologous to the host in its untransformed state, in reading phase with the structural gene for the heterologous DNA; 2. Cloning vehicles coding for the microbial expression of a protein variously comprising (a) a poly- peptide hapten and additional protein suffi- cient in size to confer immunogenicity on the product of expression, which may find use in raising antibodies to the hapten for assay use or in the manufacture of vaccines; and (b) a desired polypeptide product and additional protein from which the desired product may be cleaved; and 3. Methods of preparing synthetic structural genes coding for the expression of mammalian polypep- tides in microbial cloning systems.

4362867

RECOMBINANT cDNA CONSTRUCTION METHOD

AND HYBRID NUCLEOTIDES USEFUL IN CLONING

Gary V. Paddock, assigned to Research Corporation

/ (d~Ooy

(rN)# i I

\(dl~)ey Compounds useful as complementary DNA (cDNA) include deoxyribonucleotides and at least one ribonucleotide. They may be depicted by the general formula: wherein (dN)a and (dN)c represent series of deoxyri- bonucleotides and (rN)b represents a series of ribonucleotides; wherein a, b, and c are the number of nucleotides in the series, with the proviso that b is > or = 1, a is > or : 35, and c is > or : 10; wherein the series of

deoxyribonucleotides (dN)a includes a ser- ies of deoxyribonucleotides which is sub- stantially complementry to the series of deoxyribonucleotides (dN)c and the dashed line represents noncovalent bonding between the complementary deoxyribonucleotide series: and wherein the solid line represents a covalent phosphodiester bond. These compounds may be prepared from mes- senger R NA (m R NA) containing the genetic information necessary for celhilar produc- tion of desired products such as polypep- tides. After appropriate modification, they may be combined with DNA from a suit- able cloning vehicle such as a plasmid and the resulting combined DNA used to trans- form bacterial cells. The transformed bacte- rial cells may then be grown and harvested; and the desired product or products recovered.

4362817

HYBRID PLASMID A N D PROCESS OF MAKING SAME

Fritz Reusser, assigned to The Upjobn Company

i I

--'-i

A novel chemical compound , plasmid pUC 1060, which was constructed from Strep- tomyces sp. 3022a chromosomal DNA and plasmid pBR322. Hybrid plasmid pUC 1060 contains a functional tet gene promoter

PATENT ABSTRACTS 99

composed of streptomycete and E. coli DNA, and, thus, is useful as a cloning vehicle in recombinant DNA work. For example, using well known DNA methodology, a desired gene, for example, the insulin gene, can be inserted into pUCI060 and the resulting plasmid can then be transformed into a suitable host microbe which, upon cultur- ing, produces the desired insulin.

4362816

HYBRID PLASMID A N D PROCESS OF MAKING SAME

Fritz Reusser, assigned to The Upjohn Company

i i = =_

ii i i ]

A novel chemical compound , plasmid pUCI031, which was constructed from Strep- tomyces sp. 3022a chromosomal DNA and plasmid pBR322. Hybrid plasmid pUC 1031 contains a functional tet gene promoter composed of streptomycete and E. coil DNA, and, thus, is useful as a cloning vehicle in recombinant DNA work. For example, using well known DNA methodology, a desired gene, for example, the insulin gene, can be inserted into pUCI031 and the resulting plasmid can then be transformed into a suitable host microbe which, upon cultur- ing, produces the desired insulin.

4360597

STREPTOMYCES PLASMID AND CULTURE

Mervyn J. Bibb, David A. Hopwood, assigned to National Research Development Corporation

PCT No. PCT/GB79/00095 Sec. 371 Date Jan. 24, 1980 Sec. 102(e) Date 3an. 24, 1980 PCT Filed Jun. I, 1979 PCT Pub. No. W079/01169 PCT Pub. Date Dec. 27, 1979. Novel Streptomyces Plasmids have the char- acteristic that their presence in non-integrated form in a micro-organism of the species Streptomyces lividans confers on that micro- organism the properties (a) of forming pocks when grown on a lawn of that strain of micro-organism deposited with the National Collection of Industrial Bacteria (NCIB) under the reference number 11416, and (b) of not forming pocks when grown on a lawn of that strain of micro-organism deposited with the NCIB under the reference number 114[7, or which is derivable from a plasmid having such a characteristic by the removal or addition of DNA therefrom. The plas- raids are prepared from micro-organisms containing them or by the manipulation of other plasmids of the group and are of value as vectors for the introduction of nucleic acid into micro-organisms.

4359535

AUTONOMOUSLY REPLICATING DNA CONTAINING INSERTED DNA

SEQUENCES

George Pieczenik

Autonomously replicating DNA containing a unique nucleotide sequence, being an oli- gonucleotide of which its sequence does not otherwise exist in said DNA, inserted in a non-essential region thereof at a site not previously susceptible of restriction endonu- clease cleavage.