LACE 2013

19th Latin-American Symposium

on Biotechnology, Biomedical, Biopharmaceutical, and Industrial

Applications of Capillary Electrophoresis and Microchip Technology

November 29 – December 3, 2013 JW Marriott Hotel, Lima, Peru

Technical Program

AGENDA OF SESSIONS November 29 – December 3, 2013

FRIDAY, November 29: At JW Marriott Hotel Lima, 4th floor, Salón Bolognesi/Pizarro

08:00 – 18:00 Registration (Foyer) 09:00 – 13:00 Pre-Symposium Course 13:00 – 14:00 Lunch Recess 14:00 – 18:00 Pre-Symposium Course

SATURDAY, November 30:

08:00 – 13:00 Registration (Foyer) 09:00 – 13:00 Pre-Symposium Course

OPENING SESSION At JW Marriott Hotel Lima, 4th floor, Salón San Martín

15:00 – 16:00 Registration (Foyer) and Poster Set-up (La Terraza del Mar) 16:00 – 16:15 Welcoming Remarks

Marina F.M. Tavares, University of Sao Paulo, Brazil Norberto A. Guzman, Princeton Biochemicals Inc., U.S.A.

PLENARY LECTURES SESSION I – Sponsored by Beckman Coulter, Inc. and AirBus Military Chairperson, Dr. Marina F. M. Tavares

16:15 – 16:55 A novel detection scheme for DNA in a spinning polyester-toner (PeT)

microdevice (OP-A-01) James P. Landers, U.S.A. 17:00 – 17:40 Metabolic tracing and metabolomics in a clinical setting (OP-A-02) Katja B.P. Elgstoen, Norway 17:45 – 18:25 Development of proteomic methods by immunoaffinity capillary

electrophoresis to monitor normal and disease profiles of the human gastrointestinal tract using stool samples (OP-A-03)

Norberto A. Guzman, U.S.A. 18:30 – 20:00 Cocktails and Instrumentation Exposition (La Terraza del Mar)

SUNDAY, December 1:

08:00 – 18:30 Registration (Foyer) and Poster Set-up (La Terraza del Mar) 08:00 – 09:00 Short Course for the Pharmaceutical Sciences of Peru (Salón San Martín)

SESSION II Chairperson, Dr. Emanuel Carrilho

9:00 – 9:20 Multi-segment injection-capillary electrophoresis-mass spectrometry: a

high-throughput platform and improved data workflow in metabolomics for biomarker discovery and personalized medicine (OP-A-04)

Philip Britz-McKibbin, Canada 9:25 – 9:45 Quantitative proteomics using CESI-MS: SILAC labeled yeast as a

model system (OP-A-05) Herbert Lindner, Austria

9:50 – 10:10 Pseudostationary phase mediated open tubular capillary

electrochromatography for n-glycan analysis (OP-A-06) Andras Guttman, Hungary 10:15 – 10:35 Expanding the role of capillary electrophoresis for the development,

characterization and control of therapeutic biologics (OP-A-07) Jeffrey D. Chapman, U.S.A. 10:40 – 11:20 Coffee Break, POSTER PRESENTATION I (PP-A-01 to PP-A-10), and

Instrumentation Exhibition (La Terraza del Mar)

SESSION III Chairperson, Dr. Nora M. Vizioli

11:20 – 11:40 Microchip electrophoresis with electrochemical detection for monitoring

biological processes in cells and freely roaming animals (OP-A-08) Susan M. Lunte, U.S.A. 11:45 – 12:05 Selection of aptamers using capillary electrophoresis methods and their

applications in biosensors (OP-A-09) Sam F.Y. Li, Singapore 12:10 – 12:30 Enantiopurity analysis of anti-AIDS drugs and related acyclic nucleoside

phosphonates-based antivirotics by capillary electrophoresis (OP-A-10) Václav Kašička, Czech Republic

12:30 – 14:30 Lunch Recess Short course for the Pharmaceutical Sciences of Peru (Salón San Martín)

SESSION IV Chairperson, Dr. Virginia Robinson-Fuentes

14:30 – 14:50 Microscale chemical analyses with multifunctional nanogels:

programming biomimetic nanomaterials to process and quantify biomarkers (OP-A-11)

Lisa A. Holland, U.S.A. 14:55 – 15:15 Capillary electrophoresis with evaporative light scattering detection:

some analytical possibilities for solving specific problems (OP-A-12) Angel Ríos, Spain 15:20 – 15:40 Wax printing in bioanalyis and point-of-care diagnostic

devices (OP-A-13) Emanuel Carrilho, Brazil

15:40 – 16:20 Coffee Break, POSTER PRESENTATION II (PP-A-11 to PP-A-20), and

Instrumentation Exhibition

SESSION V Chairperson, Dr. Lucas Blanes

16:20 – 16:40 Separation of DNA by sequence in CZE (OP-A-14) Linda B. McGown, U.S.A. 16:45 – 17:05 Detection modes for Capillary Electrophoresis (OP-A-15) Craig E. Lunte, U.S.A. 17:10 – 17:30 CE-MS as a component of a multiplatform approach to disease

biomarkers discovery through metabolomics (OP-A-16) Coral Barbas, Spain

17:30 – 18:30 Instrumentation Exhibition & Short course for the Pharmaceutical Sciences of Peru (Salón San Martín) 20:00 Leasure activity

MONDAY, December 2:

08:00 – 18:30 Registration SESSION VI Chairperson, Dr. María S. Aurora-Prado

9:00 – 9:20 Microfluidic devices integrating solid-phase extraction, fluorescence

labeling, and microchip electrophoresis separation (OP-A-17) Adam T. Woolley, U.S.A. 9:25 – 9:45 Phenolic compounds analysis in foods by CE compared with HPLC

(OP-A-18) Helena T. Godoy, Brazil 9:50 – 10:10 Characterization of a lauryl acrylate porous polymer monolithic

stationary phase: diffusion, retention, and thermodynamic studies via capillary electrochromatography and HPLC (OP-A-19)

Michelle M. Bushey, U.S.A. 10:15 – 10:35 Study of the methylation profile of DNA from brain tissues of rats treated

with zinc telluride (OP-A-20) Nora M. Vizioli, Argentina 10:40 – 11:20 Coffee Break, POSTER PRESENTATION IV (PP-A-21 to PP-A-30),

and Instrumentation Exhibition

SESSION VII Chairperson, Dr. Patricia W. Stege

11:20 – 11:40 Rapid diagnosis of galactosemia diseases in newborn urine samples on

microfluidic chips-copper nanowires platforms (OP-A-21) Alberto Escarpa, Spain 11:45 – 12:05 Quantitative capillary zone electrophoresis method for the precise

determination of charge differences arising from the manufacture of a lysosomal enzyme (OP-A-22)

Robert Weinberger, U.S.A. 12:10 – 12:30 Enantioselective fungal biotransformation of hydroxyzine and analysis

by capillary electrophoresis (OP-A-23) Anderson R.M. de Oliveira, Brazil

12:30 – 14:30 Lunch Recess

SESSION VIII Chairperson, Dr. Marcone A.L. de Oliveira

14:30 – 14:50 Capillary electrophoresis analysis of different species of Trichoderma

SPP. (OP-A-24) Virginia A. Robinson-Fuentes, Mexico 14:55 – 15:15 Joining forces of CE-ICP-MS and ESI-MS/MS techniques in explanation

of the anticancer ruthenium-based drug mechanism of action (OP-A-25) Magdalena Matczuk, Poland 15:20 – 15:40 Potentiality of a reversed flow CE-UV and LC-MS/MS methods for the

characterization of prostate cancer biomarkers (OP-A-26) Ana Valéria C. Simionato, Brazil

15:40 – 16:20 Coffee Break, POSTER PRESENTATION IV (PP-A-31 to PP-A-50),

and Instrumentation Exhibition SESSION IX Chairperson, Dr. Helena T. Godoy

16:20 – 16:40 Implementation of carbon-based nanomaterials in capillary

electrochromatography as an immunosupport (OP-A-27) Patricia W. Stege, Argentina 16:45 – 17:05 A portable explosive detector based on fluorescence quenching of pyrene

deposited on coloured wax printed uPADs (OP-A-28) Lucas Blanes, Brazil

17:10 – 17:30 Then and now; Beckman Coulter and Capillary Electrophoresis (TP-A-01)

Ed Horton, U.S.A. 17:30 – 18:30 Instrumentation Exhibition (please remove all posters at the end of

session)

20:00 Gala Dinner

TUESDAY, December 3:

08:00 – 13:00 Registration SESSION X Chairperson, Dr. Anderson R.M. de Oliveira

9:00 – 9:20 Monitoring the production of the Brazilian Meningococcal C conjugate

vaccine by CE (OP-A-29) Shirley de M.P. Abrantes, Brazil 9:25 – 9:45 Capillary electrophoresis- mass spectrometry for the characterization of

glycoproteins and glycan derivatives (OP-A-30) Roxana G. Jayo, Canada 9:50 – 10:10 Fatty acids analysis by capillary electrophoresis associate to

chemometrics approach: an interesting alternative (OP-A-31) Marcone A.L. de Oliveira, Brazil 10:15 – 10:35 A novel optimization method for separation of complex mixtures in CE.

Application to the analysis of Harmala alkaloids in edible algae by CE-MS. (OP-A-32)

Marcos Tascon, Argentina 10:40 – 11:20 Coffee Break and Instrumentation Exhibition

SESSION XI Chairperson, Dr. Ana Valéria C. Simionato

11:20 – 11:40 Capillary electrophoresis as a guide in the isolation of active ingredients

from plants and as a tool for determination of physical and chemical properties related to absorption, metabolism and excretion of the active isolates (OP-A-33)

Gustavo A. Micke, Brazil

11:45 – 12:05 Chiral capillary electrophoresis and its role in cutting-edge research of charged helical molecules (OP-A-34)

Dušan Koval, Czech Republic 12:10 – 12:30 Targeted and untargeted approaches to clinical metabolomics (OP-A-35) Marina F. M. Tavares, Brazil 12:30 – 13:00 Closing Remarks and Photography Session

Poster Presentations Panel support for poster presentations can fit posters having the following dimensions: 90 cm (35.4 inches) width x 120 cm (47.2 inches) height. Three Poster Prizes will be awarded for excellent presentation of particularly significant innovative research during poster sessions. The awards are sponsored by Analytical and Bioanalytical Chemistry and entitle the winners a free selection of books published by Springer up to a value of 200 euro. PP-A-01 METABOLOMIC APPROACH TO SEARCHING FOR BLADDER CANCER

BIOMARKERS USING CE-MS Juliana V. Alberice, Antonia García-Fernández, Nuria Malats, Coral Barbas, and Emanuel Carrilho (Brazil & Spain)

PP-A-02 PRELIMINARY STEREOSELECTIVE BIOTRANSFORMATION STUDY OF ZOPICLONE EMPLOYING FUNGI AND ANALYSIS BY CAPILLARY ELECTROPHORESIS Nayara C.P. Albuquerque and Anderson R.M. de Oliveira (Brazil)

PP-A-03 SIMULTANEOUS DETERMINATION OF VILDAGLIPTIN AND METFORMIN IN COATED TABLET FORMULATION BY CAPILLARY ELECTROPHORESIS Amanda T. Barden, Bruna L. Piccoli, Nadia M. Volpato, María S. Aurora-Prado, and Martin Steppe (Brazil)

PP-A-04 THE INFLUENCE OF THE SIZE OF MAGNETIC SILICA BEADS ON THE DNA EXTRACTION ON PT-MICRODEVICE Juliane C. Borba, Kimberly Jackson, Qian Liu, James P. Landers, and Emanuel Carrilho (Brazil & U.S.A.)

PP-A-05 DEVELOPMENT OF ANALYTICAL PAPER-BASED MICROFLUIDIC

DEVICES TO DETECT AND QUANTIFY ETHANOL Rubiane Borba, Emanuel Carrilho, Mafalda Costa, João J.I. Silva, and Elvira Fortunato (Brazil & Portugal)

PP-A-06 DEVELOPMENT OF A MEKC SYSTEM APPLIED TO THE QUALITY CONTROL OF URSODEOXYCHOLIC ACID IN PHARMACEUTICAL FORMULATIONS AND RAW MATERIAL Oriana Boscolo, Sabrina Flor, Manuela Martinefski, Valeria Tripodi, and Silvia Lucangioli (Argentina)

PP-A-07 CE AS A TOOL IN A PHYSICAL-CHEMISTRY PROPERTY STUDY: APPLICATION IN ALKALOIDS ISOLATED FROM Senecio brasiliensis Lizandra C. Bretanha, Gustavo A. Micke, and Moacir G. Pizzolatti (Brazil)

PP-A-08 OPTIMIZATION OF EXTRACTION AND MEASUREMENT OF PHENOLIC

COMPOUNDS IN EDIBLE MUSHROOMS BY CAPILLARY ELECTROPHORESIS Francisco A. de S. Campos Jr, Mateus H. Petrarca, Adriana D. Meinhart, Adriana T. Godoy, and Helena T. Godoy (Brazil)

PP-A-09 13C LABELED COMPOUNDS IN THE ELUCIDATION OF METABOLIC

PATHWAYS: AN APPLICATION BY CE-MS IN METABOLOMICS ANALYSIS Gisele A.B. Canuto, Emerson A. Castilho-Martins, Marina F.M. Tavares, Luis Rivas, Ángeles López-Gonzálvez, and Coral Barbas (Brazil & Spain)

PP-A-10 COUPLING POLYESTER-TONER MICROCHIP ELECTROPHORESIS WITH ELECTROSPRAY IONIZATION MASS SPECTROMETRY FOR BIOCOMPOUNDS ANALYSIS Jonatan R. Catai, Suzyeth M. Melo, and Emanuel Carrilho (Brazil)

PP-A-11 TECHNOLOGICAL RISKS IN ENVIRONMENTAL CHANGE AND GOVERNMENTAL DIMENSION AND POLICY Sedat Cereci (Turkey)

PP-A-12 REVERSED FLOW CE-UV WITH INDIRECT DETECTION FOR AMINO ACIDS ANALYSIS: A METHOD TO INVESTIGATE AMYOTROPHIC LATERAL SCLEROSIS BIOMARKERS Zuzana Cieslarová, and Ana Valéria C. Simionato (Brazil)

PP-A-13 PHOSPHOLIPID-ASSISTED CAPILLARY ELECTROPHORESIS FOR

ENZYMATIC CHARACTERIZATION AND ASSAY Cassandra Crihfield, Srikanth Gattu, Anthony Moncrief, and Lisa Holland (U.S.A.)

PP-A-14 SOLVENT EFFECTS ON THE MEKC SEPARATION OF DRUGS OF ABUSE Jose Luiz Costa, André R. Morrone, Rodrigo R. Resende, Alice A. da M. Chasin, Marina F.M. Tavares, and João P.S. Farah (Brazil)

PP-A-15 TARGETED METABOLOMICS ANALYSIS BY CE-DAD IN HUMAN URINE Pedro L.R. da Cruz, Aline Klassen, Andrea T. Faccio, and Marina F.M. Tavares (Brazil)

PP-A-16 PHOSPHOLIPID NANOGEL AIDED MICROFLUIDIC SEPARATION FOR DNA BIOMARKER IDENTIFICATION Tyler Davis, Lisa Holland, and Cassandra Crihfield (U.S.A.)

PP-A-17 ELECTROCHEMICAL DETECTION FOR MICROCHIP - CAPILLARY ELECTROPHORESIS FIVE SQUARE PLATFORM Fabiane C. de Abreu, and Carlos D. Garcia (Brazil & U.S.A.)

PP-A-18 A DRUG RELEASE STUDY FROM TAIOBA STARCH USING QSRR

MODELING Maicon de Morais, Lucas Sobral, João P.S. Farah, and Marina F.M. Tavares (Brazil)

PP-A-19 CAPILLARY ZONE ELECTROPHORESIS AS AN ALTERNATIVE METHOD FOR DETERMINATION OF FUROSEMIDE IN RAW MATERIAL Aline de Souza, Laura V. Español-Mariño, Claudia V. de Oliveira, María S. Aurora-Prado, Maria Inês R.M. Santoro, and Erika R.M. Kedor-Hackmann (Brazil)

PP-A-20 PROTEOME STUDY FROM EXTRACELLULAR AND MAJOR CELLULAR PROTEINS FROM Xylella fastidiosa CULTIVATED IN DIFFERENT CULTURE MEDIA USING OFFGEL Fayene Z.R. de Souza, Regiane de F. Travensolo, and Emanuel Carrilho (Brazil)

PP-A-21 DEVELOPMENT OF A MICROFLUIDIC DEVICE TO STUDY Xylella fastidiosa BACTERIUM COLONIZATION Fayene Z.R. de Souza, Regiane de F. Travensolo, and Emanuel Carrilho (Brazil)

PP-A-22 EVALUATION OF FREE COMPONENTS IN THE BRAZILIAN MENINGOCOCCAL C CONJUGATE VACCINE USING CE METHODS Iaralice M. de Souza, Milton N. da Silva, Elza C.S. Figueira, Maria de Lourdes M. Leal, Ellen Jessouroum, Shirley de M.P. Abrantes, and Ivna A.F.B. da Silveira (Brazil)

PP-A-23 USING CAPILLARY ELECTROPHORESIS FOR THE CHARACTERIZATION OF A WATER-SOLUBLE POLYAMPHOLYTE Laura A. del Rio, Lucía Lombardo Lupano, Juan Lázaro Martínez, Viviana Campodall’ Orto, and Nora M. Vizioli (Argentina)

PP-A-24 CAPILLARY ELECTROPHORESIS AS A POWERFUL TOOL TO SCREENING BIOACTIVE COMPOUNDS IN PLANTS EXTRACTS: APPLICATION IN Senecio brasiliensis Maressa D. Dolzan, Jacqueline P. Vistuba, Lizandra Bretanha, Elis C.R. da Silva, and Gustavo A. Micke (Brazil)

PP-A-25 DETERMINATION OF THE CONTAMINANT DIMETHYLAMINOPROPYLAMINE (DMAPA) IN ALKYL AMIDOPROPYL BETAINE SURFACTANT BY CAPILLARY ELECTROPHORESIS WITH INDIRECT UV DETECTION Fábio C. dos Santos, Aline Klassen, Livio C. da Rocha, and Marina F.M. Tavares (Brazil)

PP-A-26 DETERMINATION OF THE DISSOCIATION CONSTANTS OF SELECTED FLAVONOIDS BY CZE Thais D. dos Santos, Daniel R. Oliveira, João P.S. Farah, and Marina F.M. Tavares (Brazil)

PP-A-27 DEVELOPMENT OF A CAPILLARY ELECTROPHORESIS METHOD FOR QUANTITATIVE DETERMINATION OF DRUGS IN NANOPARTICLES Laura V. Español, Aline de Souza, Marina F.M.Tavares, Erika R.M. Kedor-Hackmann, Maria Inês R.M. Santoro, and María S. Aurora-Prado (Brazil)

PP-A-28 CHARACTERIZATION OF A MEKC SYSTEM BASED ON POLYMERIC-MIXED MICELLES Sabrina Flor, Matías Iglesias Rando, Mario Contin, Valeria Tripodi, and Silvia Lucangioli (Argentina)

PP-A-29 CAPILLARY ZONE ELECTROPHORESIS STUDY OF ACARBOSE UNDER STRESS CONDITIONS Angel A. Gaona-Galdos, María S. Aurora-Prado, Maria Inês R.M. Santoro, and Erika R.M. Kedor-Hackmann (Brazil)

PP-A-30 POLYMERIC NANOPARTICLES AS PSEUDOSTATIONARY PHASE FOR THE SEPARATION OF SYNTHETIC PEPTIDES BY CAPILLARY ELECTROCHROMATOGRAPHY Denise A. Grela, Daiana Collazo, and Nora M. Vizioli (Argentina)

PP-A-31 OPTIMIZATION OF AN ANALYTICAL METHOD FOR THE ANALYSIS OF TOPIRAMATE BY CAPILLARY ELECTROPHORESIS WITH INDIRECT UV DETECTION Aline A. Ishikawa, Thiago P. Segato, Lanuze G.B. de Toni, Anderson R.M. de Oliveira, Marina F.M. Tavares, Emanuel Carrilho, and Cristiane M. de Gaitani (Brazil)

PP-A-32 CAPILLARY ELECTROPHORESIS METHOD DEVELOPMENT FOR THE ANALYSIS OF DANOFLOXACIN IN FISH TISSUE IN RESIDUES DEPLETION STUDIES Valquiria A.P. Jabor, Luis Fernando L. Guimarães, Severino M. de Alencar, and Jonas A.R. Paschoal (Brazil)

PP-A-33 NOVEL STATIONARY PHASES FOR CAPILLARY ELECTROCHROMATOGRAPHY: 1. PSEUDOSTATIONARY PHASE APPROACH FOR CARBOHYDRATE ANALYSIS Marta Kerekgyarto, and Andras Guttman (Hungary)

PP-A-34 CE-UV AND LC-MS METHODS FOR AROMATIC AMINES AS A MEANS OF CONFIRMING AZO DYES PRESENCE IN TEXTILE MATRICES Aline Klassen, Daniel R. Oliveira, João P.S. Farah, and Marina F.M. Tavares (Brazil)

PP-A-35 GLASS/SU-8 MICROCHIP FOR ELECTROKINETIC APPLICATIONS Paulo A.G.C. Leão, Renato S. Lima, Alessandra M. Monteiro, Maria Helena de O. Piazzetta, Ângelo L. Gobbi, Luiz Henrique Mazo, and Emanuel Carrilho (Brazil)

PP-A-36 USE OF OFFGEL/CE-DAD FOR ASSESSMENT OF PROTEIN PROFILING OF ALBUMEN SAMPLES Fabiana A. Marques, Juliana V. Alberice, Adriano Aquino, and Emanuel Carrilho (Brazil)

PP-A-37 DETERMINATION OF LOW MOLAR WEIGHT ALDEHYDES IN INDOOR ENVIRONMENTS BY CAPILLARY ELECTROPHORESIS AFTER DERIVATIZATION WITH NASH’S REAGENT Elisabete A. Pereira, and Darllene Silveira (Brazil)

PP-A-38 EVALUATION OF THERMODYNAMIC PARAMETERS IN TERPENE-SDS MICELLE INTERACTION VIA MEKC Carolina R.C. Picossi, Daniel R. Oliveira, João P.S. Farah, and Marina F.M. Tavares (Brazil)

PP-A-39 URINARY BIOMARKERS FOR CHRONIC RENAL DESEASE IN DOGS: FIBRINOGEN Virginia A. Robinson-Fuentes, Angélica Gutiérrez-Cancino, and Salvador Padilla Arellanes (Mexico)  

PP-A-40 SYSTEMATIC DEVELOPMENT OF CAPILLARY ELECTROPHORESIS PROTOCOLS FOR THE ANALYSIS OF PHARMACEUTICALS FROM DISTINCT CHEMICAL CLASSES Karina T. Rodrigues, Pedro L.R. da Cruz, Loraine R. Togni, Daniel R. Oliveira, Thaiani K.V. de Oliveira, Karen M. Ranocchia, Aline Scarabelin, Larissa R. Prestes, and Marina F.M. Tavares (Brazil)

PP-A-41 DEVELOPMENT AND OPTIMIZATION OF A CE-MS METHOD FOR AMINO ACIDS TARGETED METABOLOMICS ANALYSIS IN A VESICOURETERAL REFLUX PILOT STUDY Karina T. Rodrigues, Aline de P. Vitor, Denis T.R. Vidal, Daniela Daniel, Edgar P. Moraes, João P.S. Farah, Claudimir L. do Lago, Marcelo de S. Tavares, and Marina F. M. Tavares (Brazil)

PP-A-42 RAPID ANALYTICAL PROCEDURE FOR DETERMINATION OF A SCHISTOSOMIASIS DRUG BY FSCE WITH DIODE ARRAY DETECTION Elenice L. da S. Santos, Erika R.M. Kedor-Hackmann, Maria Inês R.M. Santoro, and María S. Aurora-Prado (Brazil)

PP-A-43 MISIDENTIFICATION OF LYSINE/ARGININE METHYLATION BY MASS SPECTROMETRY Bettina Sarg, Shadab A. Birgani, Gerald Brosch, Klaus Faserl, and Herbert Lindner (Austria)

PP-A-44 ANALYSIS OF THE IONIC BEHAVIOR OF THE ASSOCIATION OF HEXAMETHYLATED P-SULFONATOCALIX[6]ARENE (SC6HM) WITH THE SURFACTANT DODECYLTRIMETHYLAMMONIUM BROMIDE (C12TABR) BY CAPILLARY ELECTROPHORESIS Daniel A. Spudeit, Juliana B. Dalmarco, Leandro Scorsin, Samantha Gonçalves, Gustavo A. Micke, and Faruk Nome (Brazil)

PP-A-45 MICROFLUIDIC POLYESTER-BASED DEVICES AS PLATFORMS FOR CELL CULTURE Ana Carolina Urbaczek, Fayene Z.R. de Souza, Beatriz N.M. de Miranda, Lívia C. de A. Ribeiro, Emanuel Carrilho, and Iracilda Z. Carlos (Brazil)

PP-A-46 DETERMINATION OF KAEMPFEROL AND QUERCETIN IN LEAVES EXTRACT OF Maytenus ilicifolia BY CE-UV USING DIFFERENT FACTORIAL DESIGNS IN THE OPTIMIZATION OF SEPARATION, ACID HYDROLYSIS AND EXTRACTION PROCESS Jacqueline P. Vistuba, Samantha Gonçalves, Maressa D. Dolzan, Marcel Piovezan, Luciano Vitali, Fabiano C. Bertoldi, Marcone A.L. de Oliveira, Ana Carolina O. Costa, and Gustavo A. Micke (Brazil)

PP-A-47 DEVELOPMENT AND VALIDATION OF A BIOANALYTICAL METHOD USING SUPPORTED LIQUID EXTRACTION (SLE) FOR QUANTIFICATION OF FLUVASTATIN ENANTIOMERS IN PLASMA BY ELECTROKINETIC CHROMATOGRAPHY (EKC) Jennifer M.C. Yokoya, Cristiane M. de Gaitani, and Anderson R.M. de Oliveira (Brazil)