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15-18 November 2011
Genetic Laboratory
Forest Research Institute Malaysia (FRIM) Kuala Lumpur
WORKSHOP ON MICROSATELLITE DNA PROFILING AND
PLANT GENETIC TRANSFORMATION
Date: 15 – 18 November 2011 Venue: Genetic Laboratory, Forest Research Institute Malaysia (FRIM)
Kuala Lumpur ___________________________________________________________________
Title: Prof. Assoc. Prof. Dr. Mr. Ms.
Full name: _________________________________________________
Organisation:_________________________________________________
Address: _________________________________________________
____________________________________________________________
____________________________________________________________
Tel:____________________________ Fax: ________________________
Email: ______________________________________________________
Registration fee: RM1,800.00 I enclose a cheque/money order/postal order/bank draft (No: _______) for
the amount of RM__________as payment for participation in the workshop.
The payment should be made payable to the ‘DIRECTOR GENERAL FRIM’.
Signature: ________________________Date:______________________
Please send the completed form along with the registration fee to:
The Secretariat Workshop on Microsatellite DNA Profiling and Plant Genetic Transformation, Forest Research Institute Malaysia (FRIM), 52109 Kepong, Selangor, MALAYSIA. Email: [email protected] or [email protected]
(Attn.: Dr. Siti Salwana Hashim and Mr. Mohd Rosli Haron)
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WORKSHOP ONWORKSHOP ON
MICROSATELLITE DNA MICROSATELLITE DNA MICROSATELLITE DNA PROFILING AND PLANT PROFILING AND PLANT PROFILING AND PLANT
GENETIC TRANSFORMATIONGENETIC TRANSFORMATIONGENETIC TRANSFORMATION
Introduction
Precise, fast, cost-effective, and reliable identification of new plant varieties is essen-tial in agro-forestry as well as for practical breeding purposes and plant proprietary rights protection. Conventionally, morphological descriptors are routinely used to establish the identity of varieties, but these morphological descriptors suffer from many drawbacks such as influence of environment on trait expression, epistatic inter-action and pleiotropic effects. The Protection of New Plant Varieties Act 2004 of Ma-laysia provides for the protection of breeders for their contribution towards the crea-tion of new plant varieties. Application for registration of a new plant variety shall be supported by documents and information relating to the characteristics of the plant variety which can be achieved using DNA profiling. DNA profiling (also called DNA testing, DNA typing, or DNA fingerprinting) is the iden-tification of individuals on the basis of their respective DNA profiles. The method of DNA profiling used today is based on polymerase chain reaction (PCR) and commonly uses microsatellite markers. Microsatellite markers (also called short tandem repeats or simple sequence repeats) are polymorphic DNA markers that contain a repeated nucleotide sequence. The repeat can be from 2–7 nucleotides in length. There are tens of thousands of microsatellites in the plant genome. The majority of the mi-crosatellite regions have a number of different alleles, each with a different number of repeat units. Microsatellite analysis involves PCR amplification of the microsatellite regions using fluorescently labelled primers, and the PCR products are then analysed by electrophoresis to separate the alleles according to size. The polymorphisms dis-played at each microsatellite region are by themselves very common; typically each
polymorphism will be shared by around 5–20% of individuals. When looking at multi-ple loci, it is the unique combination of these polymorphisms to an individual that makes this method discriminating as an identification tool. Genetic transformation is the genetic alteration of an organism resulting from the uptake, genomic incorporation and expression of foreign genetic material (DNA), and thereby alters its genotype or genetic make-up. This technique plays a central role in both basic and applied molecular biology. The application of this technique has pro-duced significant achievements for major food crops and also in the overproduction of valuable plant secondary metabolites. Plant genetic transformation can be carried out by physical, chemical and biological agents. Among the methods used for plant ge-netic transformation are Agrobacterium mediated gene transfer, electroporation, microinjection, polyethylene glycol mediated gene transfer, viral transduction and particle bombardment. The particle bombardment via biolistic/gene gun is developed to enable penetration of cell wall so that genetic material containing a gene of interest can be transferred into a cell. This method has led to the development of many im-proved plant varieties with desired characteristics. At the Genetic Laboratory, Forest Research Institute Malaysia (FRIM), we routinely perform plant DNA/RNA extraction, DNA sequencing, and microsatellite genotyping and have used such techniques to generate information useful to biologists to help manage species of concern. Using our highly efficient rapid-capture technique, we have been successful in isolating microsatellite markers from numerous plant species (Shorea leprosula, S. lumutensis, S. platyclados, Koompassia malaccensis, Hopea bilitonensis, Gonystylus bancanus, Johannesteijsmannia lanceolata and Eurycoma longifolia). These markers have been effectively used in population studies, timber tracking and identification of individuals. In addition, by using the particle bombard-ment method, we have produced transgenic teaks which are resistant to the larvae of Paliga damastesalis.
Individuals and professionals in government organizations, research institutions,
universities and private companies involved in research on plant population genetics,
plant breeding, plant variety identification, timber tracking and genetic engineering.
This four-days workshop will be held on 15 – 18 November 2011, at the Genetic Laboratory, Forest Research Institute Malaysia (FRIM), Selangor, Malaysia.
Session 1 (Day 1–2): Plant DNA extraction; What is microsatellite?; How to de-velop microsatellite markers; Microsatellite genotyping; Microsatellites for genetic conservation; Microsatellites for paternity analysis; Microsatellites for plant variety identification; and Microsatellites for forensic applications.
Session 2 (Day 3–4): Plant RNA extraction; Principles of plant tissue culture; Ge-netic transformation techniques; Particle bombardment; The use of vectors, pro-moters and selectable markers; and Evaluation of transgenic material (Southern, Western and PCR).
Participants may contact the following hotels directly for reservations: Crystal Crown Hotel (603-62594422); Legend Hotel (603-40429888); Vistana Hotel (603-40428000); and Dynasty Hotel (603-40437777)
Dr. Lee Soon Leong (Tel: 603-62797145; Email: [email protected]) or Dr. Norwati Adnan (Tel: 603-62797151; Email: [email protected]).
The workshop will consist of lectures and hands-on practicals. The registration fee of RM1,800 is inclusive of lecture notes, hands-on materials and meals. Please submit payment enclosed with the completed registration form and all payments should be made in the form of cheque/money order/postal order/bank draft to the DIRECTOR GENERAL FRIM.
To provide participants with the opportunity to gain a theoretical insight into the
technologies used in DNA profiling and genetic transformation.
To provide participants with hands-on experience in DNA profiling using mi-
crosatellites and genetic transformation techniques.
Objectives
Who Should Attend?
Dates and Venue
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