143:4, 2010 ESVP/ECVP Proceedings 2010 345

P8GASTRIC POLYPS AND SPONTANEOUS HELICOBACTER

SPP. INFECTION IN DOGSM. Taulescu, C. Catoi, P. Bolfa, L. Ciobanu, A. Gal

and F. TabaranUniversity of Agricultural Sciences and Veterinary Medicine, Romania

Introduction: The objective of this study was to evaluate the mor-phological aspects of gastric polyps and the prevalence of Helicobacterspp. infection in dogs with these lesions.Materials and Methods: Ten dogs (5 males and 5 females) witha mean age of 11 years (range 9e16 years) were diagnosed with gas-tric polyps on routine necropsy examination. The polyps wereremoved and examined histologically. The presence of Helicobacterspp. was investigated by Giemsa staining and immunohistochemistrywith polyclonal anti-Helicobacter pylori (ab7788, ABCAM).Results: The polyps were classified as hyperplastic (6/10) and be-nign lymphoid (2/10). Leiomyoma was detected in two cases. Giemsastaining revealed the presence of Helicobacter spp. in eight cases. A pos-itive reaction for H. pylori by IHC was detected in three cases.Conclusion: Gastric polyps are found in dogs, but are more fre-quently diagnosed during necropsy examination of the stomach. Mi-croscopical examination is important for differentiating the type ofgastric polyps. We found a correlation between the development ofgastric polyps and spontaneous gastric infection with Helicobacterspp. in dogs.

P9VALVULAR AND PARIETAL ENDOCARDITIS IN A BENGAL

TIGERE. Ciobotaru, T. Soare, G. Dinescu, C. Constantinescu

and M. MilitaruFaculty of Veterinary Medicine, Romania

Introduction: Previous pathological studies of captive tigers have re-ported gastrointestinal diseases due to general adaptation and bacte-rial (anthrax, tuberculosis, salmonellosis) and viral diseases (caninedistemper, rhinotracheitis, calicivirus, panleukopenia, feline infec-tious peritonitis) as most frequently encountered in this species.Materials and Methods: A 16-year-old female Bengal tiger(Panthera tigris tigris) clinically diagnosed with chronic renal failurewas submitted for necropsy examination. Cytological investigationsweremade of transudate sampled from the peritoneal cavity and thorax(May Gr€unwald Giemsa stained). Samples of organs were subjected toroutine histopathology (Masson’s trichrome stain). Gaffney’s 1 hGiemsa stain was used for identification of bacterial colonies.Results: The most important gross and microscopical lesions in thisanimal included: blunted teeth with pulp cavity exposure associatedwith periodontal disease and gingivitis, endocarditis confined to thetricuspid and aortic valve and the parietal endocardium of right ven-tricle, myocardial fibrosis, ascites, hydrothorax, interstitial nephritisand proliferative glomerulonephritis, uraemic enteropathy and gas-tropathy, haemorrhages and infarction of spleen, embolic pneumoniaand bronchointerstitial pneumonia. Bacterial colonies were identifiedin the endocardium and lung.Conclusions: Prolonged antigenemia leading to a high concentra-tion of soluble immune complexes was likely to be associated withthe observed dental and endocardial injuries. Thus, glomerulonephri-tis may be the consequence of periodontal disease and endocarditis.Bacterial endocarditis and subsequent cardiac failure were likelyresponsible for the observed pulmonary inflammation.

P10PATHOMORPHOLOGICAL CHANGES IN THE LIVER OFCARP (CYPRINUS CARPIO L.) ARE DEPENDENT ON FISH

PRODUCTION TECHNOLOGYJ. Szarek*, I. Babicska*, K. Skibniewska*, J. Guziur*,

M. Gesek* and A. Andrzejewskay*University of Warmia and Mazury and yMedical University, Poland

Introduction: The morphological changes in the liver of carp(Cyprinus carpio L.) reared by semi-extensive (natural feed) and low-intensity (natural feed supplemented with granulated cereal fodder)fish production technology was compared.Materials and Methods: The study was conducted on 120 2- and3-year-old carp divided into four groups (n5 30): control fish fromtwo farms fed with natural feed (benthos); fish from two further farmsfed according to low-intensity technology and additionally fed withgranulated Aller-Aqua. HE and PAS staining of liver sections andultrastructural examinations were performed.Results: In all groups the most common changes were parenchymaldegeneration and steatosis simplex (especially in groups 2 and 4).Other lesions were present sporadically in groups 3 and 4. The glyco-gen content in the hepatocytes of all fish was similar. Among the ul-trastructural lesions present in all of the groups, steatosis simplexdominated, especially in fish of groups 2 and 4. In addition, smallchanges in the mitochondria and in rough endoplasmic reticulumwere observed, especially in older carp.Conclusion: Slightly fewer morphological lesions were observed inthe carp fed with natural feed than in those fed with granulatedfeed. Fewer morphological lesions were observed in 2-year-old fishthan in 3-year-old fish.

P11DESCRIPTION OF INTESTINAL EMPHYSEMA AMONGPIGS FROM A SLAUGHTERHOUSE IN OKLAHOMA

R.E. Mendes Ricardo*, M.T. Collens y and J.F. Edwardsz*University of Cordoba, Spain, yUniversity of Wisconsin

and zTexas A&M University, USAIntroduction: Intestinal emphysema (IE) is of low incidence, but isa well-recognized lesion of slaughter pigs, first described in 1737. Lit-tle is known about its histology, and the cause is undetermined.Materials and Methods: Over 3 days, 54,000, 110e125 kg pigswere examined. Cases of IE were identified and the viscera were ex-amined. Samples of intestines, liver, heart, mesenteric lymph node,spleen and pancreas were collected for light microscopy. Sampleswere stained with HE, Gram, GMS and acid fast techniques, andtested by PCR for Mycobacterium spp.Results: Seven cases clustered during times of collection, but pigscame from a wide geographical distribution. Mild chronic serositiswas common, but two cases also had multiple abdominal abscesses.All pigs had granulomatous mural enteritis and serositis with air trap-ped, presumably in lymphatics and lymph nodes. The enteric mucosaand all other organs examined were normal, except for the presence ofhepatic and splenic pyogenic abscesses in two cases. These abscessesseemed unrelated to the IE. Mycobacterial testing and special stainswere negative.Conclusions: IE is a segmental and granulomatous enteric and peri-toneal condition of swine. It is not an artefact of slaughter because it isassociated with a granulomatous process that exists prior to timepassed during transport and killing.