Understanding exocytosis in immune and inflammatory cells: The molecular basis of mediator secretion

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  • J ALLERGY CLIN IMMUNOL May 2003 933

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    CME itemsQuestion 1. Soluble NSF attachment protein receptors

    (SNAREs) are:A. membrane-associated proteins essential for the

    downstream fusion of granules/vesicles with their target (plasma membrane)

    B. cytosolic proteins that are recruited to docked granules/vesicles at the plasma membrane

    C. membrane-associated proteins involved in facili-tating mobilization of granules/vesicles to the cell periphery during exocytosis

    D. cytoplasmic proteins involved in recycling dock-ing complexes after granule/vesicle membrane fusion with the plasma membrane

    Question 2. The SNARE complex:A. is a trimolecular interaction of two Q-SNAREs

    and one R-SNARE and is resistant to cleavage by botulinum and tetanus neurotoxins

    B. is a trimolecular interaction of one Q-SNARE and two R-SNAREs and is resistant to cleavage by botulinum and tetanus neurotoxins

    C. is critical for exocytosis but is not implicated in other vesicle trafficking events

    D. is sensitive to proteolytic cleavage to botulinum and tetanus neurotoxins

    Question 3. R-SNAREs and Q-SNAREs:A. are both expressed in inflammatory cell types

    and always localize to specific subcompartmentsB. are expressed separately in different cell types

    but not together in the same cell typeC. are both expressed in inflammatory cell types

    and sometimes localize to specific subcompart-ments

    D. are predicted to be the prime candidates involved in directing the fusion of specific gran-ule populations with the plasma membrane

    Question 4. Administration of tetanus or botulinumtoxins into inflammatory cell types:

    A. cleaves free SNARE proteins and impairs mobil-ity of granules/vesicles to the cell periphery

    B. cleaves complexed SNAREs and impairs exocy-tosis of granule/vesicle stored mediators

    C. cleaves free SNAREs and impairs exocytosis of granule/vesicle stored mediators

    D. has a negligible effect on mediator secretion from inflammatory cell types

    Continuing Medical Education examination

    Understanding exocytosis in immune andinflammatory cells: The molecular basisof mediator secretion

    Instructions for category 1 Continuing Medical Education credit

    The American Academy of Allergy, Asthma and Immunology is accredited as a provider of Continuing MedicalEducation (CME) by the Accreditation Council for Continuing Medical Education.

    Test ID no.: mai0010Contact hours: 1.0Expiration date: April 30, 2004

    Category I credit can be earned by reading the text material and taking this CME examination online. For completeinstructions, visit the Journals Web site at www.mosby.com/jaci.

    Learning objectives: Understanding exocytosis in immune and inflammatory cells:The mole-cular basis of mediator secretion

    1. To identify the major patterns of exocytosis in inflammatory cell types and how this process is central to the devel-opment of all inflammatory conditions.

    2. To understand the critical involvement of soluble NSF (N-ethylmaleimidesensitive factor) attachment proteinreceptor (SNARE) complexes involved in granule/vesicle docking and how the diversity of SNARE isoformsmight, in part, contribute to the release of specific stored mediators from inflammatory cell types.

    3. To identify upstream signaling molecules that are implicated in regulating granule/vesicle docking and SNAREcomplex assembly.

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