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System Overview TCS SP5 II Leica TCS SP5 II, TCS SP5 MP, TCS SP5 X, FLIM, SMD

TCS SP5 II System Overview - Leica Microsystems TCS SP5 II/Brochur… · System Overview TCS SP5 II Objectives w. M25 mount Optical outfit DM6000 CFS 1565006 12 IR Black Optical Table

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Page 1: TCS SP5 II System Overview - Leica Microsystems TCS SP5 II/Brochur… · System Overview TCS SP5 II Objectives w. M25 mount Optical outfit DM6000 CFS 1565006 12 IR Black Optical Table

System Overview TCS SP5 IILeica TCS SP5 II, TCS SP5 MP, TCS SP5 X, FLIM, SMD

Page 2: TCS SP5 II System Overview - Leica Microsystems TCS SP5 II/Brochur… · System Overview TCS SP5 II Objectives w. M25 mount Optical outfit DM6000 CFS 1565006 12 IR Black Optical Table

22

ContentStands 3

Leica DM6000 CFS 4

Laser Safety Boxes 5

Scan Head Options 6

Stages & Inserts 8

With/without SuperZ 10

Environmental Control 11

MP Lasers, IR Tables/Compensor 12

OPO 14

NDD (Non Descanned Detectors) 15

FLIM (B&H) 16

APD Imaging 18

Leica TCS SMD FCS (PQ) 20

Leica TCS SMD FLIM: Internal FLIM (PQ) 22

Leica TCS MSD FLIM: Internal FLIM (PQ) 24

Leica TCS SMD FLIM: FCS + External FLIM (PQ) 26

Leica TCS MSD FLIM: FCS + Internal + External SP FLIM (PQ) 28

Page 3: TCS SP5 II System Overview - Leica Microsystems TCS SP5 II/Brochur… · System Overview TCS SP5 II Objectives w. M25 mount Optical outfit DM6000 CFS 1565006 12 IR Black Optical Table

3

0005M

D/0006M

D acieL

Stands

Le

ica

DM

I600

0

Microscope TablePassive Upright

156504701

Microscope TableActive Upright

156504715

DM6000 CS 5 pos fluo155931620

DM6000 CS 8 pos fluo155931625

DM6000 CS 5 pos fluo155931624

DM5000 CS 5 pos fluo155931521

Microscope TableActive Inverted

156504715

System Overview TCS SP5 II

EL6000 Optical Outfit DM6000156500613

EL6000 Optical Outfit DM6000 w. DIC156500614

EL6000 Optical Outfit DM5000156500603

EL6000 Optical Outfit DM5000 w. DIC156500604

EL6000 Optical Outfit DMI6000156500620

EL6000 Optical Outfit DMI6000 with DIC 156500621

DMI6000 CS Bino155933633

DMI6000 CS Trino155933621

DMI6000 CS AFC Trino155933641

Microscope TablePassive Inverted

156504711

Air Compressor forActive Tables

156502536

Air Compressor forActive Tables

156502536DMI6000 CS AFC Bino

155933640

Page 4: TCS SP5 II System Overview - Leica Microsystems TCS SP5 II/Brochur… · System Overview TCS SP5 II Objectives w. M25 mount Optical outfit DM6000 CFS 1565006 12 IR Black Optical Table

44

Leica DM6000 CFS

Optical Table 1200 x 1500 mm DM 6000 CFS

156502558

Mandatory for MP systems

Single Objective Slider15555089

Automated 2 position nosepiece15524006

System Overview TCS SP5 II

Objectives w. M25 mount

Optical outfit DM6000CFS 156500612

IR Black Optical Table DM6000 CFS

156502563DM6000 CFS basic

incl. mech. Stage

155931627

Objective Nosepiece H (6 pos, M25, mot.)

15501249

HCX APO L 20x/1.015507701

HCX APO L 20x/0.95 15507702

Dodt Contrast Illumination CFS

156504204

Dodt Contrast Illumination CFS mot

156504205

BF detector Dodt contrast156504203

Data Aquisition Unit/16ch

156504764*

ICT set for CFS nosepiece basic

156500718

ICT set CFS 20x/1.0 W /

Cond S1/0.90 1156500716

ICT set CFS 20x/1.0 W / Cond S1/1.40 Oil

156500717

IICTset CFS20x/1.0 W/Cond S1/0.90 enh. sep.

1156500719

Scanning stage CFS flat 156504163

Stage BMmot 7238 step CFS 15501250

Luigs & Neumann Stage in vitro 156504160

Luigs & Neumann Stage in vivo 156504162

Inserts: please have a look at

Page xxx

Slice Mini Badkammer II for L&N Stage in vitro

156504164

Tandem scanningsystem SP5 II

156501122

Conventional scanningsystem SP5 II

156501120

Resonant scanningsystem SP5 II

156501121

TCS MP Unitfor DM6000 CFS

156508002

Laser Safety Boxes CFS

Laser Safety Box CFS blackStage 2010156506059

Laser Safety Box CFS blackL&N 2010156506058

Please make sure to include a laser safety box

MP Lasers:Please have a look at

page 12

Trigger unit156504760

*requires 64-bit Windows, to be released soon

Page 5: TCS SP5 II System Overview - Leica Microsystems TCS SP5 II/Brochur… · System Overview TCS SP5 II Objectives w. M25 mount Optical outfit DM6000 CFS 1565006 12 IR Black Optical Table

5

Leica DM6000 CFS

Optical Table 1200 x 1500 mm DM 6000 CFS

156502558

Mandatory for MP systems

Single Objective Slider15555089

Automated 2 position nosepiece15524006

System Overview TCS SP5 II

Objectives w. M25 mount

Optical outfit DM6000CFS 156500612

IR Black Optical Table DM6000 CFS

156502563DM6000 CFS basic

incl. mech. Stage

155931627

Objective Nosepiece H (6 pos, M25, mot.)

15501249

HCX APO L 20x/1.015507701

HCX APO L 20x/0.95 15507702

Dodt Contrast Illumination CFS

156504204

Dodt Contrast Illumination CFS mot

156504205

BF detector Dodt contrast156504203

Data Aquisition Unit/16ch

156504764*

ICT set for CFS nosepiece basic

156500718

ICT set CFS 20x/1.0 W /

Cond S1/0.90 1156500716

ICT set CFS 20x/1.0 W / Cond S1/1.40 Oil

156500717

IICTset CFS20x/1.0 W/Cond S1/0.90 enh. sep.

1156500719

Scanning stage CFS flat 156504163

Stage BMmot 7238 step CFS 15501250

Luigs & Neumann Stage in vitro 156504160

Luigs & Neumann Stage in vivo 156504162

Inserts: please have a look at

Page xxx

Slice Mini Badkammer II for L&N Stage in vitro

156504164

Tandem scanningsystem SP5 II

156501122

Conventional scanningsystem SP5 II

156501120

Resonant scanningsystem SP5 II

156501121

TCS MP Unitfor DM6000 CFS

156508002

Laser Safety Boxes CFS

Laser Safety Box CFS blackStage 2010156506059

Laser Safety Box CFS blackL&N 2010156506058

Please make sure to include a laser safety box

MP Lasers:Please have a look at

page 12

Trigger unit156504760

*requires 64-bit Windows, to be released soon

Page 6: TCS SP5 II System Overview - Leica Microsystems TCS SP5 II/Brochur… · System Overview TCS SP5 II Objectives w. M25 mount Optical outfit DM6000 CFS 1565006 12 IR Black Optical Table

66

Scan Head Options

Basic optical module VISIRwith scan rotation

156501107

System Overview TCS SP5 II

Basic optical module HIVISwith scan rotation

156501106

Basic Confocal ModuleAOBS

156501103

Basic optical module HIVISwith scan rotation

15651106

Basic optical module VISIRwith scan rotation

156501107

UV/405 Port156501201

Attenuation FW405156502351

Tandem ScanningSystem SP5156501122

Conventional ScanningSystem SP5156501120

Resonant ScanningSystem SP5156501121

Confocal DetectorImaging

156501390

MFP and IR Lasers(see page 12 ff.)

Imaging WLL w.Ar ion laser156502148

Imaging WLL156502149

Basic Confocal ModuleDichroic

156501104

Basic optical module HIVISwith scan rotation

156501106

Basic optical module VISIRwith scan rotation

156501107

Basic Confocal Module WLL

156501105

Imaging Laser AOBS R 156502100RB 156502105RGB 156502110RYB 156502115RYBV 156502116ROB 156502120ROGB 156502123ROYB 156502125ROYBV 156502126

Imaging Laser Dichroic R 156502150G 156502151RB 156502155RGB 156502160RYB 156502165RYBV 156502166ROB 156502170ROGB 156502173ROYB 156502175ROYBV 156502176

UV and Laser Options

AOTF 405 Internal156502352

Pulsed 405 Laser 156502305

405 Laser156502300

UV Laser 405Laser AOTF incl.

156502326*

SP Channel 01 156501310SP Channel 02 156501320SP Channel 03 156501330SP Channel 04 156501340SP Channel 05 156501350

Confocal DetectorFLIM

156501391

Hybrid GaAsPdetector SP f. imaging

158001392

Adaptor kit HyD SP for Basic Module 158*

158001492

Switchable Beam Expander

156502220

Exhaust w. Silencer Ar Laser

156502210

X1 Port Options (see page 16 ff.)

Control Panel w. Programmable LCD

156504750

Trigger unit 156504760

NDD Detector Options(see page 15 ff.)

Double filter wheelbasic incl. analyzer

158004505

Notch filter set VIS for SP5156504511

Notch filter set STED158004507

Barrier filter set MP158004508

Notch filter set SMD158004509

UV and UV/405 Options(see page 6)

* available on special request (SPR)

Page 7: TCS SP5 II System Overview - Leica Microsystems TCS SP5 II/Brochur… · System Overview TCS SP5 II Objectives w. M25 mount Optical outfit DM6000 CFS 1565006 12 IR Black Optical Table

7

Scan Head Options

Basic optical module VISIRwith scan rotation

156501107

System Overview TCS SP5 II

Basic optical module HIVISwith scan rotation

156501106

Basic Confocal ModuleAOBS

156501103

Basic optical module HIVISwith scan rotation

15651106

Basic optical module VISIRwith scan rotation

156501107

UV/405 Port156501201

Attenuation FW405156502351

Tandem ScanningSystem SP5156501122

Conventional ScanningSystem SP5156501120

Resonant ScanningSystem SP5156501121

Confocal DetectorImaging

156501390

MFP and IR Lasers(see page 12 ff.)

Imaging WLL w.Ar ion laser156502148

Imaging WLL156502149

Basic Confocal ModuleDichroic

156501104

Basic optical module HIVISwith scan rotation

156501106

Basic optical module VISIRwith scan rotation

156501107

Basic Confocal Module WLL

156501105

Imaging Laser AOBS R 156502100RB 156502105RGB 156502110RYB 156502115RYBV 156502116ROB 156502120ROGB 156502123ROYB 156502125ROYBV 156502126

Imaging Laser Dichroic R 156502150G 156502151RB 156502155RGB 156502160RYB 156502165RYBV 156502166ROB 156502170ROGB 156502173ROYB 156502175ROYBV 156502176

UV and Laser Options

AOTF 405 Internal156502352

Pulsed 405 Laser 156502305

405 Laser156502300

UV Laser 405Laser AOTF incl.

156502326*

SP Channel 01 156501310SP Channel 02 156501320SP Channel 03 156501330SP Channel 04 156501340SP Channel 05 156501350

Confocal DetectorFLIM

156501391

Hybrid GaAsPdetector SP f. imaging

158001392

Adaptor kit HyD SP for Basic Module 158*

158001492

Switchable Beam Expander

156502220

Exhaust w. Silencer Ar Laser

156502210

X1 Port Options (see page 16 ff.)

Control Panel w. Programmable LCD

156504750

Trigger unit 156504760

NDD Detector Options(see page 15 ff.)

Double filter wheelbasic incl. analyzer

158004505

Notch filter set VIS for SP5156504511

Notch filter set STED158004507

Barrier filter set MP158004508

Notch filter set SMD158004509

UV and UV/405 Options(see page 6)

* available on special request (SPR)

Page 8: TCS SP5 II System Overview - Leica Microsystems TCS SP5 II/Brochur… · System Overview TCS SP5 II Objectives w. M25 mount Optical outfit DM6000 CFS 1565006 12 IR Black Optical Table

88

Stages & Inserts

System Overview TCS SP5 II

SuperZ Galvo Stage Type S

156504102Motor Stage upright*

156504155

Manual Stage 15501234

Scanning Stage upright 156504150

Insert rotatable Type S156504104

Insert fix Type S156504103

Insert universal Type S 156504105

SuperZ Galvo Stage Type H

156504116

Motor Stage inverted156504145

Manual Stage*15522019

Scanning Stage universal 156504141

Insert rotatable Type H156504114

Insert fix Type H156504113

Insert universal Type H 156504115

Leic

a D

M60

00/D

M50

00Le

ica

DM

I600

0

Petri Dish Insert 39 mm156504139

Petri Dish Insert 36 mm156504136

Insert SuperZ Willco Dish 35 mm

156604421

Insert SuperZ Mattek Dish 35 mm

156604423

Insert SuperZ Willco Dish 50 mm

156604422

Plate insert SuperZ Microtiter Plate

156504420

Inserts for SuperZ

Stages and Inserts

Inserts for use w/o SuperZ

Inserts for Scanning Stage upright w/o SuperZ

Inserts for Scanning Stage inverted w/o SuperZ

Glas Plate 160x116x311600183

Universal Specimenholder156504171

Holder for Glas Slides 3“x1“ 11600184

Heatable Mounting FrameAK 160x16156506040

Holder forSlide Frames 70x70mm

11600185

Insert univ. forMicrotiter Plates

15532338

Universal Holding Frame 15600234

*Default

*Default

Page 9: TCS SP5 II System Overview - Leica Microsystems TCS SP5 II/Brochur… · System Overview TCS SP5 II Objectives w. M25 mount Optical outfit DM6000 CFS 1565006 12 IR Black Optical Table

9

Stages & Inserts

System Overview TCS SP5 II

SuperZ Galvo Stage Type S

156504102Motor Stage upright*

156504155

Manual Stage 15501234

Scanning Stage upright 156504150

Insert rotatable Type S156504104

Insert fix Type S156504103

Insert universal Type S 156504105

SuperZ Galvo Stage Type H

156504116

Motor Stage inverted156504145

Manual Stage*15522019

Scanning Stage universal 156504141

Insert rotatable Type H156504114

Insert fix Type H156504113

Insert universal Type H 156504115

Leic

a D

M60

00/D

M50

00Le

ica

DM

I600

0

Petri Dish Insert 39 mm156504139

Petri Dish Insert 36 mm156504136

Insert SuperZ Willco Dish 35 mm

156604421

Insert SuperZ Mattek Dish 35 mm

156604423

Insert SuperZ Willco Dish 50 mm

156604422

Plate insert SuperZ Microtiter Plate

156504420

Inserts for SuperZ

Stages and Inserts

Inserts for use w/o SuperZ

Inserts for Scanning Stage upright w/o SuperZ

Inserts for Scanning Stage inverted w/o SuperZ

Glas Plate 160x116x311600183

Universal Specimenholder156504171

Holder for Glas Slides 3“x1“ 11600184

Heatable Mounting FrameAK 160x16156506040

Holder forSlide Frames 70x70mm

11600185

Insert univ. forMicrotiter Plates

15532338

Universal Holding Frame 15600234

*Default

*Default

Page 10: TCS SP5 II System Overview - Leica Microsystems TCS SP5 II/Brochur… · System Overview TCS SP5 II Objectives w. M25 mount Optical outfit DM6000 CFS 1565006 12 IR Black Optical Table

1010

With/without SuperZ

System Overview TCS SP5 II

SuperZ Galvo Stage Type H

156504116

SuperZ Incubation System extended (110 V)

156506061

SuperZ IncubationSystem Basic

156506060

SuperZ Incubation System Extended (230 V)

156506062

Heating Insert P 156506014

On Stage Incubation SystemComplete

156506010

Cool/Heat Insert P156506015

Wit

h Su

perZ

Petri Dish attachment35 mm SuperZ

156506064

Petri Dish attachment35 mm SuperZ

156506063

Attachment forChamber Slide SuperZ

156506065

Attachment forSlide Glas SuperZ

156506067

Attachment forCover Glas Chamber SuperZ

156506066

On Stage Incubator(Incubator S)

156506013

Environmental Control

Climate Chambers for DM6000 CFS

Climate Chambers for DMI6000

Ludin Cube & BoxSP5 II internal HyD

156506080

Ludin Cube2 & BoxDMI6000, bright

156506007

Conv. Kit LudinCube & Box PMT /HyD

156506081

See page 5in section DMI6000 CFS

Incubator BL-TIRF LSM DMI6000, black

156506019

Wit

hout

Sup

erZ

Temp Control for Objective Heater

156506030

Objective Heaterd= 29 mm 156506031

Objective Heaterd= 30,5 mm15650632

Perfusion Insert f. POC-R (open w. cover)

15521752

POC-R Cell Cultivation System

w/o SuperZ156506022

Cult Foil, 25 pc. for POC-R) 15521754

Perfusion Insert f. POC mini(open w. cover)

15521740

POC mini Cell Cultivation System

156506022

Foil cover for POC mini 15521741

SuperZ Galvo Stage Type H

156504116

POC-R Cell Cultivation System

w. SuperZ156506020

Perfusion Insert f. POC-R (open w. cover)

15521752

Cult Foil, 25 pc. for POC-R)15521754

Ludin Chamber Set (18 mm)for DMI6000156506070

Ludin Chamber Set (12 mm)for DMI6000156506071

Holder f. Ludin Chambers for SuperZ and rot. insert

156506073

Holder f. Ludin ChambersAdapter f. SuperZ

156506074

Holder f. Ludin Chambersw/o SuperZ156506072

Passive Gas RegulationLUDIN Cube & Box without

LUDIN incubator kit 156506051

Active CO2 RegulationLUDIN Cube & Box without

LUDIN incubator kit 156506050

O2 Controller 2 – 60%for LUDIN Active

156506004

O2 Controller 0.5 – 25%for LUDIN Active

156506006

Incubator Kit LUDINfor use without SuperZ

156506053

Incubator Kit LUDINfor use with SuperZ

156506052

Page 11: TCS SP5 II System Overview - Leica Microsystems TCS SP5 II/Brochur… · System Overview TCS SP5 II Objectives w. M25 mount Optical outfit DM6000 CFS 1565006 12 IR Black Optical Table

11

With/without SuperZ

System Overview TCS SP5 II

SuperZ Galvo Stage Type H

156504116

SuperZ Incubation System extended (110 V)

156506061

SuperZ IncubationSystem Basic

156506060

SuperZ Incubation System Extended (230 V)

156506062

Heating Insert P 156506014

On Stage Incubation SystemComplete

156506010

Cool/Heat Insert P156506015

Wit

h Su

perZ

Petri Dish attachment35 mm SuperZ

156506064

Petri Dish attachment35 mm SuperZ

156506063

Attachment forChamber Slide SuperZ

156506065

Attachment forSlide Glas SuperZ

156506067

Attachment forCover Glas Chamber SuperZ

156506066

On Stage Incubator(Incubator S)

156506013

Environmental Control

Climate Chambers for DM6000 CFS

Climate Chambers for DMI6000

Ludin Cube & BoxSP5 II internal HyD

156506080

Ludin Cube2 & BoxDMI6000, bright

156506007

Conv. Kit LudinCube & Box PMT /HyD

156506081

See page 5in section DMI6000 CFS

Incubator BL-TIRF LSM DMI6000, black

156506019

Wit

hout

Sup

erZ

Temp Control for Objective Heater

156506030

Objective Heaterd= 29 mm 156506031

Objective Heaterd= 30,5 mm15650632

Perfusion Insert f. POC-R (open w. cover)

15521752

POC-R Cell Cultivation System

w/o SuperZ156506022

Cult Foil, 25 pc. for POC-R) 15521754

Perfusion Insert f. POC mini(open w. cover)

15521740

POC mini Cell Cultivation System

156506022

Foil cover for POC mini 15521741

SuperZ Galvo Stage Type H

156504116

POC-R Cell Cultivation System

w. SuperZ156506020

Perfusion Insert f. POC-R (open w. cover)

15521752

Cult Foil, 25 pc. for POC-R)15521754

Ludin Chamber Set (18 mm)for DMI6000156506070

Ludin Chamber Set (12 mm)for DMI6000156506071

Holder f. Ludin Chambers for SuperZ and rot. insert

156506073

Holder f. Ludin ChambersAdapter f. SuperZ

156506074

Holder f. Ludin Chambersw/o SuperZ156506072

Passive Gas RegulationLUDIN Cube & Box without

LUDIN incubator kit 156506051

Active CO2 RegulationLUDIN Cube & Box without

LUDIN incubator kit 156506050

O2 Controller 2 – 60%for LUDIN Active

156506004

O2 Controller 0.5 – 25%for LUDIN Active

156506006

Incubator Kit LUDINfor use without SuperZ

156506053

Incubator Kit LUDINfor use with SuperZ

156506052

Page 12: TCS SP5 II System Overview - Leica Microsystems TCS SP5 II/Brochur… · System Overview TCS SP5 II Objectives w. M25 mount Optical outfit DM6000 CFS 1565006 12 IR Black Optical Table

1212

Air compressorfor active tables

156502536

IR Tables/CompressorMP Picosecond: Lasers and Beam Routing

H

igh

pow

er la

sers

Tandem ScanningSystem SP5156501122

IR Laser MaiTai HP156502503

IR Laser ChameleonUltra II

156502508

IR Beam Routingw. EOM DM6000

158002593

IR Beam Routingw. EOM DMI6000

158002594

IR Beam Routingw. EOM CFS158002595

IR Imaging Multifunc. Port HX

156501216

IR Imaging Multifunc. Port inv. HX156501206

IR Imaging Multifunc. Port upright CFS HX

156501217

IR Tables/Compressor

sresal rewop

woL

IR Laser MaiTai156502514

IR black optical table DM6000900 x 1200156502561

IR black optical table DMI6000900 x 1500156502560

IR black optical table DM6000 CFSand STED 1200 x 1500

156502563

IR Imaging Multifunc. Port upright HX

156501216

IR Beam Routingw. EOM DM6000

158002593

System Overview TCS SP5 II

Optical AdapterMaiTai Leica156502567

MP: Femtosecond Lasers, Beam, Routing, Optical, Adapters

H

igh

pow

er la

sers

IR Laser MaiTai HP156502513

Tandem ScanningSystem SP5156501122

IR Laser ChameleonUltra

156502516

Optical adapterMaiTai HP Leica

156502565

Optical adapterChameleon Leica

156502566

Optical adapter MaiTaiHP other supplier

156502568High power laser

purchased directly from laser supplier

by customer

Optical adapter Cha-meleon other supplier

156502569

IR Imaging Multifunc. Port upright HX

156501216

IR Imaging Multifunc. Port inv. HX156501206

IR Imaging Multifunc. Port CFS HX156501217

IR Beam Routingw. EOM DM6000

158002593

IR Beam Routingw. EOM DMI6000

158002594

IR Beam Routingw. EOM CFS158002595

IR Laser ChameleonUltra II

156502518

IR black optical table DMI6000900 x 1500156502560

IR black optical table DM6000900 x 1200156502561

IR black optical table DM6000 CFSand STED 1200 x 1500

156502563

Air compressorfor active tables

156502536

MP Femtosecond, Lasers, Routing, Optical Adapters IR Tables/Compressor

IR black optical tableDM6000 900 x 1200

156502561

Low power laserpurchased directlyfrom laser supplier

by customer

IR Imaging Multifunc. Port inv. HX156501206

Optical AdapterMaiTai other supplier

156502567 IR Imaging Multifunc. Port CFS HX156501217

IR Beam Routingw. EOM DMI6000

158002594

IR Beam Routingw. EOM CFS158002595

IR black optical tableDMI6000 900 x 1500

156502560

IR black optical tableDM6000 CFS and STED

1200 x 1500156502563

Air compressorfor active tables

156502536

Page 13: TCS SP5 II System Overview - Leica Microsystems TCS SP5 II/Brochur… · System Overview TCS SP5 II Objectives w. M25 mount Optical outfit DM6000 CFS 1565006 12 IR Black Optical Table

13

Air compressorfor active tables

156502536

IR Tables/CompressorMP Picosecond: Lasers and Beam Routing

H

igh

pow

er la

sers

Tandem ScanningSystem SP5156501122

IR Laser MaiTai HP156502503

IR Laser ChameleonUltra II

156502508

IR Beam Routingw. EOM DM6000

158002593

IR Beam Routingw. EOM DMI6000

158002594

IR Beam Routingw. EOM CFS158002595

IR Imaging Multifunc. Port HX

156501216

IR Imaging Multifunc. Port inv. HX156501206

IR Imaging Multifunc. Port upright CFS HX

156501217

IR Tables/Compressor

sresal rewop

woL

IR Laser MaiTai156502514

IR black optical table DM6000900 x 1200156502561

IR black optical table DMI6000900 x 1500156502560

IR black optical table DM6000 CFSand STED 1200 x 1500

156502563

IR Imaging Multifunc. Port upright HX

156501216

IR Beam Routingw. EOM DM6000

158002593

System Overview TCS SP5 II

Optical AdapterMaiTai Leica156502567

MP: Femtosecond Lasers, Beam, Routing, Optical, Adapters

H

igh

pow

er la

sers

IR Laser MaiTai HP156502513

Tandem ScanningSystem SP5156501122

IR Laser ChameleonUltra

156502516

Optical adapterMaiTai HP Leica

156502565

Optical adapterChameleon Leica

156502566

Optical adapter MaiTaiHP other supplier

156502568High power laser

purchased directly from laser supplier

by customer

Optical adapter Cha-meleon other supplier

156502569

IR Imaging Multifunc. Port upright HX

156501216

IR Imaging Multifunc. Port inv. HX156501206

IR Imaging Multifunc. Port CFS HX156501217

IR Beam Routingw. EOM DM6000

158002593

IR Beam Routingw. EOM DMI6000

158002594

IR Beam Routingw. EOM CFS158002595

IR Laser ChameleonUltra II

156502518

IR black optical table DMI6000900 x 1500156502560

IR black optical table DM6000900 x 1200156502561

IR black optical table DM6000 CFSand STED 1200 x 1500

156502563

Air compressorfor active tables

156502536

MP Femtosecond, Lasers, Routing, Optical Adapters IR Tables/Compressor

IR black optical tableDM6000 900 x 1200

156502561

Low power laserpurchased directlyfrom laser supplier

by customer

IR Imaging Multifunc. Port inv. HX156501206

Optical AdapterMaiTai other supplier

156502567 IR Imaging Multifunc. Port CFS HX156501217

IR Beam Routingw. EOM DMI6000

158002594

IR Beam Routingw. EOM CFS158002595

IR black optical tableDMI6000 900 x 1500

156502560

IR black optical tableDM6000 CFS and STED

1200 x 1500156502563

Air compressorfor active tables

156502536

Page 14: TCS SP5 II System Overview - Leica Microsystems TCS SP5 II/Brochur… · System Overview TCS SP5 II Objectives w. M25 mount Optical outfit DM6000 CFS 1565006 12 IR Black Optical Table

1414

MP Femtosecond Lasers with Precompensation, Beam Routing and Optical Adapters IR Tables/Compressor

System Overview TCS SP5 II

NDD: Non-Descanned Detection

MP: OPO, Beam Routing and Optical Adapters IR Tables/Compressor

4 Channel NDD Boxwith Liquid Light Guide

156504270

NDD Detection Unit156504253

Tandem ScanningSystem SP5156501122

Filter Cube empty156504240

Resonant ScanningSystem SP5156501121

Dapi/TRITCFilter Cube156504242

Dapi/FITCFilter Cube156504241

Tandem ScanningSystem SP5156501122

Resonant ScanningSystem SP5156501121

FITC/TXRFilter Cube156504244

CFP/YFPFilter Cube156504243

DMI6000 TLD 1 Channel156504221

DM6000 CFS TLD 1 Channel incl. Mirror HousingDM

156504227

DM6000 CFS RLD 1 Channel156504236

DM6000 RLD2 Channels156504234

DMI6000 TLD2 Channels156504223

DM6000 CFS TLD 2 Channels incl. Dichroic

156504226

DM6000 CFS TLD 2 Channels incl. Mirror Housing

156504225

DMI6000 RLD 2 Channels156504233

DM6000 CFS RLD 2 Channels156504235

Filter Cubes for 2ch NDDs with SP680

Tandem ScanningSystem SP5156501122

Empty Filter Cube156504247

Resonant ScanningSystem SP5156501121

SHG440/CFP/YFP/DsRedFilter Cube Set

156500246

Dapi/FITC/TXR/Alexa633Filter Cube Set

156504245

Filter Cube Set for 4ch NDDs

DM6000 CFS Optical andMechanical Adaption TLD 4 Channels

156504278

DM6000 Optical and MechanicalAdaption RLD 4 Channels

156504276

DMI6000 Optical and Mechanical Adaption RLD 4 Channels

156504274

DM6000 CFS Optical and MechanicalAdaption RLD 4 Channels

156504279

Resonant ScanningSystem SP5156501121

DM6000 CFS TLD1 Channel156504228

DMI6000 RLD1 Channel156504231

DM6000 RLD1 Channel156504232

DM6000 TLD1 Channel156504222

FITC/TRITC filter cube is always included with 2ch NDDs

IR Laser MaiTai HPDeepSee, fs156502534

IR Laser MaiTai eHPDeepSee, fs

690 – 1040 nm156502534

Resonant ScanningSystem SP5156501121

IR Laser ChameleonVision II, fs156502533

MaiTai DeepSee/MaiTai eHP DeepSee

purchased directyfrom laser supplier

by customer

Chameleon Vision Iand II purchsed

directly from lasersupplier by customer

Resonant ScanningSystem SP5156501121

Optical AdapterChameleonVision Leica156502572

Resonant ScanningSystem SP5156501121

Optical AdapterMaiTai

DeepSee Leica156502573

Optical AdapterMaiTai Deep See

other156502575

Optical AdapterChameleonVision other156502574

IR ImagingMultifunc. Port inv. HX

156501206

IR ImagingMultifunc. Port upright HX

156501216

IR ImagingMultifunc. Port CFS HX

156501217

IR Beam Routingw. EOM DMI6000

158002594

IR Beam Routingw. EOM DM6000

158002593

IR Beam routingw. EOM CFS158002595

IR black optical table1200 x 1800156502564

IR black optical tableDM6000 CFS and STED

1200 x 150015800563

Air compressorfor active tables

156502536

Air compressorfor active tables

156502536

Beam Routing OPODMI w. single EOM

158002591IR Laser OPOChameleon Vision II

156502608

IR Imaging Multifunc. Port inv. HX156501206

Optical Adapter OPOLeica

156502598

IR Imaging Multifunc. Port CFS HX156501217

Beam Routing OPOCFS w. single EOM

158002592

Beam Routing OPOCFS w. single EOM,

RSP670 158002590

IR black optical tableOPO 1500 x 1800

156502537Optical Adapter OPOothers

156502599

IR Laser OPOChameleon Ultra II

156502594

IR Laser OPOChameleon Ultra II orVision II purchaseddirectly from laser

supplier by customer

NDD detection unit needed if more thanthree detectors (2ch + 1ch as NDD or TL-BF) are used.

Filter Cubes for 2ch NDDs with SP680

Filter Cube OPOempty

156502607

Tandem ScanningSystem SP5156501122

Resonant ScanningSystem SP5156501121

mCherry/Cy5156502605

Filter Cube 2ch OPOTRICT/Alexa633

156502604

Filter Cube 2ch OPO

Page 15: TCS SP5 II System Overview - Leica Microsystems TCS SP5 II/Brochur… · System Overview TCS SP5 II Objectives w. M25 mount Optical outfit DM6000 CFS 1565006 12 IR Black Optical Table

15

MP Femtosecond Lasers with Precompensation, Beam Routing and Optical Adapters IR Tables/Compressor

System Overview TCS SP5 II

NDD: Non-Descanned Detection

MP: OPO, Beam Routing and Optical Adapters IR Tables/Compressor

4 Channel NDD Boxwith Liquid Light Guide

156504270

NDD Detection Unit156504253

Tandem ScanningSystem SP5156501122

Filter Cube empty156504240

Resonant ScanningSystem SP5156501121

Dapi/TRITCFilter Cube156504242

Dapi/FITCFilter Cube156504241

Tandem ScanningSystem SP5156501122

Resonant ScanningSystem SP5156501121

FITC/TXRFilter Cube156504244

CFP/YFPFilter Cube156504243

DMI6000 TLD 1 Channel156504221

DM6000 CFS TLD 1 Channel incl. Mirror HousingDM

156504227

DM6000 CFS RLD 1 Channel156504236

DM6000 RLD2 Channels156504234

DMI6000 TLD2 Channels156504223

DM6000 CFS TLD 2 Channels incl. Dichroic

156504226

DM6000 CFS TLD 2 Channels incl. Mirror Housing

156504225

DMI6000 RLD 2 Channels156504233

DM6000 CFS RLD 2 Channels156504235

Filter Cubes for 2ch NDDs with SP680

Tandem ScanningSystem SP5156501122

Empty Filter Cube156504247

Resonant ScanningSystem SP5156501121

SHG440/CFP/YFP/DsRedFilter Cube Set

156500246

Dapi/FITC/TXR/Alexa633Filter Cube Set

156504245

Filter Cube Set for 4ch NDDs

DM6000 CFS Optical andMechanical Adaption TLD 4 Channels

156504278

DM6000 Optical and MechanicalAdaption RLD 4 Channels

156504276

DMI6000 Optical and Mechanical Adaption RLD 4 Channels

156504274

DM6000 CFS Optical and MechanicalAdaption RLD 4 Channels

156504279

Resonant ScanningSystem SP5156501121

DM6000 CFS TLD1 Channel156504228

DMI6000 RLD1 Channel156504231

DM6000 RLD1 Channel156504232

DM6000 TLD1 Channel156504222

FITC/TRITC filter cube is always included with 2ch NDDs

IR Laser MaiTai HPDeepSee, fs156502534

IR Laser MaiTai eHPDeepSee, fs

690 – 1040 nm156502534

Resonant ScanningSystem SP5156501121

IR Laser ChameleonVision II, fs156502533

MaiTai DeepSee/MaiTai eHP DeepSee

purchased directyfrom laser supplier

by customer

Chameleon Vision Iand II purchsed

directly from lasersupplier by customer

Resonant ScanningSystem SP5156501121

Optical AdapterChameleonVision Leica156502572

Resonant ScanningSystem SP5156501121

Optical AdapterMaiTai

DeepSee Leica156502573

Optical AdapterMaiTai Deep See

other156502575

Optical AdapterChameleonVision other156502574

IR ImagingMultifunc. Port inv. HX

156501206

IR ImagingMultifunc. Port upright HX

156501216

IR ImagingMultifunc. Port CFS HX

156501217

IR Beam Routingw. EOM DMI6000

158002594

IR Beam Routingw. EOM DM6000

158002593

IR Beam routingw. EOM CFS158002595

IR black optical table1200 x 1800156502564

IR black optical tableDM6000 CFS and STED

1200 x 150015800563

Air compressorfor active tables

156502536

Air compressorfor active tables

156502536

Beam Routing OPODMI w. single EOM

158002591IR Laser OPOChameleon Vision II

156502608

IR Imaging Multifunc. Port inv. HX156501206

Optical Adapter OPOLeica

156502598

IR Imaging Multifunc. Port CFS HX156501217

Beam Routing OPOCFS w. single EOM

158002592

Beam Routing OPOCFS w. single EOM,

RSP670 158002590

IR black optical tableOPO 1500 x 1800

156502537Optical Adapter OPOothers

156502599

IR Laser OPOChameleon Ultra II

156502594

IR Laser OPOChameleon Ultra II orVision II purchaseddirectly from laser

supplier by customer

NDD detection unit needed if more thanthree detectors (2ch + 1ch as NDD or TL-BF) are used.

Filter Cubes for 2ch NDDs with SP680

Filter Cube OPOempty

156502607

Tandem ScanningSystem SP5156501122

Resonant ScanningSystem SP5156501121

mCherry/Cy5156502605

Filter Cube 2ch OPOTRICT/Alexa633

156502604

Filter Cube 2ch OPO

Page 16: TCS SP5 II System Overview - Leica Microsystems TCS SP5 II/Brochur… · System Overview TCS SP5 II Objectives w. M25 mount Optical outfit DM6000 CFS 1565006 12 IR Black Optical Table

1616

FLIM (B&H)

Resonant ScanningSystem SP5156501121

Confocal DetectorImaging

156501390

Coupling FLIM MP156505350

X1 PortEmission access

156504500

X1 Port adaptionmultipurpose for FCS/APD imaging

and FLIM156504531

X1 Port adaptionfor FLIM

156504532

APD Detection unit156505130

SP Channel 01 – 05(See page 6)

andem ScanningSystem SP5

IR Imaging PortMultifunc.

(See page 12 ff.)

System SP5156501122UV/405 Port156501201

andem ScanningSystem SP5IR Beam Routing

(See page 12 ff.)System SP5156501122

IR Laser (See page 12 ff.) System SP5

156501122IR black optical table

(See page 12 ff.)

SMD Filter cube

156505240156505241156505242156505243156505244156505245156505246156505247156505248156505249156505250156505251156505252

EmptyAlexa 405/cy2DAPI/Atto 647CFP/YFPCFP/RFPCy2/Cy5GFP narrow/RFPGFP wide/RFPFITC/TRITCGFP/HcRedAlexa 488/Alexa 633Neutral splittingPolarization splitting

FLIM Filter cube m

156504580156504581156504582156504583156504584

MP FLIM mD FLIM mDAPI mFITC mTRITC m

B&H SPC 830 incl.PC and software

156505301

B&H Routing for4 channel detection

156505340

B&H FLIM detectorPMC-100

156505330

Pulsed Laser 405156502305

Adaptor Kit HyD SP158001492 (new systems)

or156501493 (old systems)

Confocal DetectorFLIM

156501391

Basic Confocal ModuleAOBS

+ Basic Optical Module+ Imaging Laser AOBS

(See page 6)

Trigger unit156504760

System Overview TCS SP5 II

SMD Excitation: WLL156505231

FLIM Filter cube m

156504560156504561156504562156504563156504564

MP FLIMD FLIMDAPIFITCTRITC

Basic Module Dichroic+ Basic Optical Module

+ Imaging Laser Dichroic(See page 6)

Scanhead Options

Basic Confocal ModuleWLL

156501105+ Basic Optical Module

Scanhead Options

System SP5156501122

Imaging Laser WLL2(Pulse picker)

156502138

System SP5156501122

Imaging Laser WLL2(Pulse picker) + Ar

156502139

Notch filter set MP156504508

Double filter wheel basicincl. analyzer158004505

Notch filter set SMD156504509

Notch filter set VISfor SP5

156504511

Note: 2nd generation WLL2to be released soon

Page 17: TCS SP5 II System Overview - Leica Microsystems TCS SP5 II/Brochur… · System Overview TCS SP5 II Objectives w. M25 mount Optical outfit DM6000 CFS 1565006 12 IR Black Optical Table

17

FLIM (B&H)

Resonant ScanningSystem SP5156501121

Confocal DetectorImaging

156501390

Coupling FLIM MP156505350

X1 PortEmission access

156504500

X1 Port adaptionmultipurpose for FCS/APD imaging

and FLIM156504531

X1 Port adaptionfor FLIM

156504532

APD Detection unit156505130

SP Channel 01 – 05(See page 6)

andem ScanningSystem SP5

IR Imaging PortMultifunc.

(See page 12 ff.)

System SP5156501122UV/405 Port156501201

andem ScanningSystem SP5IR Beam Routing

(See page 12 ff.)System SP5156501122

IR Laser (See page 12 ff.) System SP5

156501122IR black optical table

(See page 12 ff.)

SMD Filter cube

156505240156505241156505242156505243156505244156505245156505246156505247156505248156505249156505250156505251156505252

EmptyAlexa 405/cy2DAPI/Atto 647CFP/YFPCFP/RFPCy2/Cy5GFP narrow/RFPGFP wide/RFPFITC/TRITCGFP/HcRedAlexa 488/Alexa 633Neutral splittingPolarization splitting

FLIM Filter cube m

156504580156504581156504582156504583156504584

MP FLIM mD FLIM mDAPI mFITC mTRITC m

B&H SPC 830 incl.PC and software

156505301

B&H Routing for4 channel detection

156505340

B&H FLIM detectorPMC-100

156505330

Pulsed Laser 405156502305

Adaptor Kit HyD SP158001492 (new systems)

or156501493 (old systems)

Confocal DetectorFLIM

156501391

Basic Confocal ModuleAOBS

+ Basic Optical Module+ Imaging Laser AOBS

(See page 6)

Trigger unit156504760

System Overview TCS SP5 II

SMD Excitation: WLL156505231

FLIM Filter cube m

156504560156504561156504562156504563156504564

MP FLIMD FLIMDAPIFITCTRITC

Basic Module Dichroic+ Basic Optical Module

+ Imaging Laser Dichroic(See page 6)

Scanhead Options

Basic Confocal ModuleWLL

156501105+ Basic Optical Module

Scanhead Options

System SP5156501122

Imaging Laser WLL2(Pulse picker)

156502138

System SP5156501122

Imaging Laser WLL2(Pulse picker) + Ar

156502139

Notch filter set MP156504508

Double filter wheel basicincl. analyzer158004505

Notch filter set SMD156504509

Notch filter set VISfor SP5

156504511

Note: 2nd generation WLL2to be released soon

Page 18: TCS SP5 II System Overview - Leica Microsystems TCS SP5 II/Brochur… · System Overview TCS SP5 II Objectives w. M25 mount Optical outfit DM6000 CFS 1565006 12 IR Black Optical Table

1818

APD imaging

X1 PortEmission access

156504500

X1 Port adaptionfor APD imaging

and FCS156504530

APD Detection unit156505130

SP Channel 01 – 05(See page 6)

SMD Filter cube

156505240156505241156505242156505243156505244156505245156505246156505247156505248156505249156505250156505251156505252

EmptyAlexa 405/cy2DAPI/Atto 647CFP/YFPCFP/RFPCy2/Cy5GFP narrow/RFPGFP wide/RFPFITC/TRITCGFP/HcRedAlexa 488/Alexa 633Neutral splittingPolarization splitting

SP5 Basic Module AOBS+

Imaging Laser AOBS(See page 6)

System Overview TCS SP5 II

SP5 Basic ModuleDichroic

+Imaging Laser Dichroic

(See page 6)

Basic Confocal ModuleWLL

156501105+

Basic Optical Module

System SP5156501122

Imaging Laser WLL2156502137

System SP5156501122

Imaging Laser WLL2+ Ar

156502138

Note: 2nd generation WLL2to be released soon

Page 19: TCS SP5 II System Overview - Leica Microsystems TCS SP5 II/Brochur… · System Overview TCS SP5 II Objectives w. M25 mount Optical outfit DM6000 CFS 1565006 12 IR Black Optical Table

19

APD imaging

X1 PortEmission access

156504500

X1 Port adaptionfor APD imaging

and FCS156504530

APD Detection unit156505130

SP Channel 01 – 05(See page 6)

SMD Filter cube

156505240156505241156505242156505243156505244156505245156505246156505247156505248156505249156505250156505251156505252

EmptyAlexa 405/cy2DAPI/Atto 647CFP/YFPCFP/RFPCy2/Cy5GFP narrow/RFPGFP wide/RFPFITC/TRITCGFP/HcRedAlexa 488/Alexa 633Neutral splittingPolarization splitting

SP5 Basic Module AOBS+

Imaging Laser AOBS(See page 6)

System Overview TCS SP5 II

SP5 Basic ModuleDichroic

+Imaging Laser Dichroic

(See page 6)

Basic Confocal ModuleWLL

156501105+

Basic Optical Module

System SP5156501122

Imaging Laser WLL2156502137

System SP5156501122

Imaging Laser WLL2+ Ar

156502138

Note: 2nd generation WLL2to be released soon

Page 20: TCS SP5 II System Overview - Leica Microsystems TCS SP5 II/Brochur… · System Overview TCS SP5 II Objectives w. M25 mount Optical outfit DM6000 CFS 1565006 12 IR Black Optical Table

2020

Leica TCS SMD FCS (PQ)

X1 PortEmission access

156504500

SP Channel 01 – 05(See page 6)

Basic Confocal ModuleAOBS

+ Basic Optical Module+ Imaging Laser AOBS

(See page 6)

System Overview TCS SP5 II

Scanning stageincl. SuperZ

SuperZ Galvostage

Conventionalscanning system

156501120

Tandemscanning system

156501122

Obj. HCX PL APO63x/1.20 W CORR CS

0.14 – 0.1815506279

SMD Filter cube

156505240156505241156505242156505243156505244156505245156505246156505247156505248156505249156505250156505251156505252

EmptyAlexa 405/cy2DAPI/Atto 647CFP/YFPCFP/RFPCy2/Cy5GFP narrow/RFPGFP wide/RFPFITC/TRITCGFP/HcRedAlexa 488/Alexa 633Neutral splittingPolarization splitting

SMD Detection package FCS(high time resolution, 2 MPD SPAD)

156505200

SMD Detection package FCS(high sensitivity, 2 PE APDs)

156505201

Second license for SPT-1 (FCS)156505280

Please, order exactly one SMD detection package!An SMD excitation package is not necessary for FCS.

Resonant scanning system 156501121 cannot be used for FCS.

Page 21: TCS SP5 II System Overview - Leica Microsystems TCS SP5 II/Brochur… · System Overview TCS SP5 II Objectives w. M25 mount Optical outfit DM6000 CFS 1565006 12 IR Black Optical Table

21

Leica TCS SMD FCS (PQ)

X1 PortEmission access

156504500

SP Channel 01 – 05(See page 6)

Basic Confocal ModuleAOBS

+ Basic Optical Module+ Imaging Laser AOBS

(See page 6)

System Overview TCS SP5 II

Scanning stageincl. SuperZ

SuperZ Galvostage

Conventionalscanning system

156501120

Tandemscanning system

156501122

Obj. HCX PL APO63x/1.20 W CORR CS

0.14 – 0.1815506279

SMD Filter cube

156505240156505241156505242156505243156505244156505245156505246156505247156505248156505249156505250156505251156505252

EmptyAlexa 405/cy2DAPI/Atto 647CFP/YFPCFP/RFPCy2/Cy5GFP narrow/RFPGFP wide/RFPFITC/TRITCGFP/HcRedAlexa 488/Alexa 633Neutral splittingPolarization splitting

SMD Detection package FCS(high time resolution, 2 MPD SPAD)

156505200

SMD Detection package FCS(high sensitivity, 2 PE APDs)

156505201

Second license for SPT-1 (FCS)156505280

Please, order exactly one SMD detection package!An SMD excitation package is not necessary for FCS.

Resonant scanning system 156501121 cannot be used for FCS.

Page 22: TCS SP5 II System Overview - Leica Microsystems TCS SP5 II/Brochur… · System Overview TCS SP5 II Objectives w. M25 mount Optical outfit DM6000 CFS 1565006 12 IR Black Optical Table

2222

SMD Filter cube

156505240156505241156505242156505243156505244156505245156505246156505247156505248156505249156505250156505251156505252

EmptyAlexa 405/cy2DAPI/Atto 647CFP/YFPCFP/RFPCy2/Cy5GFP narrow/RFPGFP wide/RFPFITC/TRITCGFP/HcRedAlexa 488/Alexa 633Neutral splittingPolarization splitting

Leica TCS SMD FLIM: External FLIM (PQ)

SMD detectionpackage external

FLIM (high time resolution,

2 MPD SPAD)156505203

IRLaser

Scanning stageincl. SuperZ

SuperZGalvo stage

X1 PortEmission access

156504500

Trigger unit156504760

UV/405 Port156501201 Correction lenses

SMD Excitationpackage

(405, pulsed + cw;470 +640 pulsed)

156505224

SMD Excitationpackage

(470 + 640, pulsed)156505222

SMD Excitationpackage

(470, pulsed)156505228

SMD Excitationpackage

(440 + 470, pulsed)156505230

SMD Excitationpackage

(440, pulsed)156505229

SMD Excitationpackage

(MP)156505225

System Overview TCS SP5 II

No

Yes

MP?

SP Channel 01 – 05(See page 6)

Basic Confocal ModuleAOBS

+ Basic Optical Module+ Imaging Laser AOBS

(See page 6)

Basic Module Dichroic+ Basic Optical Module

+ Imaging Laser Dichroic(See page 6)

Basic Confocal ModuleWLL

156501105+

Basic Optical Module

SMD excitation: WLL156505231

System SP5156501122

Imaging Laser WLL2(Pulse picker)

156502138

System SP5156501122

Imaging Laser WLL2(Pulse picker) + Ar

156502139

Note: 2nd generation WLL2to be released soon

IR Imaging PortMultifunc.

(See page 12 ff.)

IR Beam Routing(See page 12 ff.)

IR Laser(See page 12 ff.)

IR black optical table(See page 12 ff.)

Notch filter set MP156504508

Notch filter set VISfor SP5

156504511

Notch filter set SMD156504509

Double filter wheel basicincl. analyzer158004505

Notch filter set VISfor SP5

156504511

SMD Excitationpackage

(405 + 470 + 640, all pulsed)156505223

SMD Excitationpackage

(405, pulsed + cw)156505221

SMD Excitationpackage

(405, pulsed)156505220

SMD detectionpackage external

FLIM (basic, 1PMT)

156505202

Second license forSPT-2 (FLIM)156505202

SMD Filter cube

156505240156505253156505254156505255156505256

EmptyUVDAPIFITCTRITC

Please, order exactly one SMD detection and one SMD excitation package!Only exception: package 156505220 or 156505221 can be combined with 156505225.

Optional with MP: pulsed 405 nm excitation

Page 23: TCS SP5 II System Overview - Leica Microsystems TCS SP5 II/Brochur… · System Overview TCS SP5 II Objectives w. M25 mount Optical outfit DM6000 CFS 1565006 12 IR Black Optical Table

23

SMD Filter cube

156505240156505241156505242156505243156505244156505245156505246156505247156505248156505249156505250156505251156505252

EmptyAlexa 405/cy2DAPI/Atto 647CFP/YFPCFP/RFPCy2/Cy5GFP narrow/RFPGFP wide/RFPFITC/TRITCGFP/HcRedAlexa 488/Alexa 633Neutral splittingPolarization splitting

Leica TCS SMD FLIM: External FLIM (PQ)

SMD detectionpackage external

FLIM (high time resolution,

2 MPD SPAD)156505203

IRLaser

Scanning stageincl. SuperZ

SuperZGalvo stage

X1 PortEmission access

156504500

Trigger unit156504760

UV/405 Port156501201 Correction lenses

SMD Excitationpackage

(405, pulsed + cw;470 +640 pulsed)

156505224

SMD Excitationpackage

(470 + 640, pulsed)156505222

SMD Excitationpackage

(470, pulsed)156505228

SMD Excitationpackage

(440 + 470, pulsed)156505230

SMD Excitationpackage

(440, pulsed)156505229

SMD Excitationpackage

(MP)156505225

System Overview TCS SP5 II

No

Yes

MP?

SP Channel 01 – 05(See page 6)

Basic Confocal ModuleAOBS

+ Basic Optical Module+ Imaging Laser AOBS

(See page 6)

Basic Module Dichroic+ Basic Optical Module

+ Imaging Laser Dichroic(See page 6)

Basic Confocal ModuleWLL

156501105+

Basic Optical Module

SMD excitation: WLL156505231

System SP5156501122

Imaging Laser WLL2(Pulse picker)

156502138

System SP5156501122

Imaging Laser WLL2(Pulse picker) + Ar

156502139

Note: 2nd generation WLL2to be released soon

IR Imaging PortMultifunc.

(See page 12 ff.)

IR Beam Routing(See page 12 ff.)

IR Laser(See page 12 ff.)

IR black optical table(See page 12 ff.)

Notch filter set MP156504508

Notch filter set VISfor SP5

156504511

Notch filter set SMD156504509

Double filter wheel basicincl. analyzer158004505

Notch filter set VISfor SP5

156504511

SMD Excitationpackage

(405 + 470 + 640, all pulsed)156505223

SMD Excitationpackage

(405, pulsed + cw)156505221

SMD Excitationpackage

(405, pulsed)156505220

SMD detectionpackage external

FLIM (basic, 1PMT)

156505202

Second license forSPT-2 (FLIM)156505202

SMD Filter cube

156505240156505253156505254156505255156505256

EmptyUVDAPIFITCTRITC

Please, order exactly one SMD detection and one SMD excitation package!Only exception: package 156505220 or 156505221 can be combined with 156505225.

Optional with MP: pulsed 405 nm excitation

Page 24: TCS SP5 II System Overview - Leica Microsystems TCS SP5 II/Brochur… · System Overview TCS SP5 II Objectives w. M25 mount Optical outfit DM6000 CFS 1565006 12 IR Black Optical Table

2424

Leica TCS SMD FLIM: Internal FLIM (PQ)

IRLaser

Scanning stageincl. SuperZ

SuperZGalvo stage

Trigger unit156504760

UV/405 Port156501201 Correction lenses

SMD Excitationpackage

(405, pulsed + cw;470 +640 pulsed)

156505224

SMD Excitationpackage

(470 + 640, pulsed)156505222

SMD Excitationpackage

(470, pulsed)156505228

SMD Excitationpackage

(440 + 470, pulsed)156505230

SMD Excitationpackage

(440, pulsed)156505229

SMD Excitationpackage

(MP)156505225

System Overview TCS SP5 II

No

Yes

MP?

SP Channel 01 – 05(See page 6)

Basic Confocal ModuleAOBS

+ Basic Optical Module+ Imaging Laser AOBS

(See page 6)

Basic Module Dichroic+ Basic Optical Module

+ Imaging Laser Dichroic(See page 6)

Basic Confocal ModuleWLL

156501105+

Basic Optical Module

SMD excitation: WLL156505231

System SP5156501122

Imaging Laser WLL2(Pulse picker)

156502138

System SP5156501122

Imaging Laser WLL2(Pulse picker) + Ar

156502139

Note: 2nd generation WLL2to be released soon

IR Imaging PortMultifunc.

(See page 12 ff.)

IR Beam Routing(See page 12 ff.)

IR Laser(See page 12 ff.)

IR black optical table(See page 12 ff.)

Notch filter set MP156504508

Notch filter set VISfor SP5

156504511

Notch filter set SMD156504509

Double filter wheel basicincl. analyzer158004505

Notch filter set VISfor SP5

156504511

SMD Excitationpackage

(405 + 470 + 640, all pulsed)156505223

SMD Excitationpackage

(405, pulsed + cw)156505221

SMD Excitationpackage

(405, pulsed)156505220

SMD detectionpackage

(Spectral FLIM1 SP FLIM PMT)

156505204 Second license forSPT-2 (FLIM)156505202

Please, order exactly one SMD detection and one SMD excitation package!Only exception: package 156505220 or 156505221 can be combined with 156505225.

Optional with MP: pulsed 405 nm excitation

SMD detectionpackage

(Spectral FLIM2 SP FLIM PMT)

156505205

Page 25: TCS SP5 II System Overview - Leica Microsystems TCS SP5 II/Brochur… · System Overview TCS SP5 II Objectives w. M25 mount Optical outfit DM6000 CFS 1565006 12 IR Black Optical Table

25

Leica TCS SMD FLIM: Internal FLIM (PQ)

IRLaser

Scanning stageincl. SuperZ

SuperZGalvo stage

Trigger unit156504760

UV/405 Port156501201 Correction lenses

SMD Excitationpackage

(405, pulsed + cw;470 +640 pulsed)

156505224

SMD Excitationpackage

(470 + 640, pulsed)156505222

SMD Excitationpackage

(470, pulsed)156505228

SMD Excitationpackage

(440 + 470, pulsed)156505230

SMD Excitationpackage

(440, pulsed)156505229

SMD Excitationpackage

(MP)156505225

System Overview TCS SP5 II

No

Yes

MP?

SP Channel 01 – 05(See page 6)

Basic Confocal ModuleAOBS

+ Basic Optical Module+ Imaging Laser AOBS

(See page 6)

Basic Module Dichroic+ Basic Optical Module

+ Imaging Laser Dichroic(See page 6)

Basic Confocal ModuleWLL

156501105+

Basic Optical Module

SMD excitation: WLL156505231

System SP5156501122

Imaging Laser WLL2(Pulse picker)

156502138

System SP5156501122

Imaging Laser WLL2(Pulse picker) + Ar

156502139

Note: 2nd generation WLL2to be released soon

IR Imaging PortMultifunc.

(See page 12 ff.)

IR Beam Routing(See page 12 ff.)

IR Laser(See page 12 ff.)

IR black optical table(See page 12 ff.)

Notch filter set MP156504508

Notch filter set VISfor SP5

156504511

Notch filter set SMD156504509

Double filter wheel basicincl. analyzer158004505

Notch filter set VISfor SP5

156504511

SMD Excitationpackage

(405 + 470 + 640, all pulsed)156505223

SMD Excitationpackage

(405, pulsed + cw)156505221

SMD Excitationpackage

(405, pulsed)156505220

SMD detectionpackage

(Spectral FLIM1 SP FLIM PMT)

156505204 Second license forSPT-2 (FLIM)156505202

Please, order exactly one SMD detection and one SMD excitation package!Only exception: package 156505220 or 156505221 can be combined with 156505225.

Optional with MP: pulsed 405 nm excitation

SMD detectionpackage

(Spectral FLIM2 SP FLIM PMT)

156505205

Page 26: TCS SP5 II System Overview - Leica Microsystems TCS SP5 II/Brochur… · System Overview TCS SP5 II Objectives w. M25 mount Optical outfit DM6000 CFS 1565006 12 IR Black Optical Table

2626

SMD Filter cube

156505240156505241156505242156505243156505244156505245156505246156505247156505248156505249156505250156505251156505252

EmptyAlexa 405/cy2DAPI/Atto 647CFP/YFPCFP/RFPCy2/Cy5GFP narrow/RFPGFP wide/RFPFITC/TRITCGFP/HcRedAlexa 488/Alexa 633Neutral splittingPolarization splitting

Leica TCS SMD FLIM: FCS + External FLIM (PQ)

SMD detectionpackage external

FLIM + FCS(high time resolution,

2 MPD SPAD)156505206

IRLaser

Scanning stageincl. SuperZ

SuperZGalvo stage

X1 PortEmission access

156504500

Trigger unit156504760

UV/405 Port156501201 Correction lenses

SMD Excitationpackage

(405, pulsed + cw;470 +640 pulsed)

156505224

SMD Excitationpackage

(470 + 640, pulsed)156505222

SMD Excitationpackage

(470, pulsed)156505228

SMD Excitationpackage

(440 + 470, pulsed)156505230

SMD Excitationpackage

(440, pulsed)156505229

SMD Excitationpackage

(MP)156505225

System Overview TCS SP5 II

No

Yes

MP?

SP Channel 01 – 05(See page 6)

Basic Confocal ModuleAOBS

+ Basic Optical Module+ Imaging Laser AOBS

(See page 6)

SMD Excitation package(MP)

156505220

IR Imaging PortMultifunc.

(See page 12 ff.)

IR Beam Routing(See page 12 ff.)

IR Laser(See page 12 ff.)

IR black optical table(See page 12 ff.)

Notch filter set VISfor SP5

156504511

Notch filter set VISfor SP5

156504511

Notch filter set SMD156504509

Double filter wheel basicincl. analyzer158004505

Notch filter set SMD156504509

SMD Excitationpackage

(405 + 470 + 640, all pulsed)156505223

SMD Excitationpackage

(405, pulsed + cw)156505221

SMD Excitationpackage

(405, pulsed)156505220

Second license forSPT-1 + 2 (FCS + FLIM)

156505282

Please, order exactly one SMD detection and one SMD excitation package!Only exception: package 156505220 or 156505221 can be combined with 156505225.

Resonant scanning system 156501121 cannot be used for FLCS.

optional

Trigger unit156504760

Conventionalscanning system

156501120

Tandemscanning system

156501122

UV/405 Port156501201Correction lenses

SMD Excitation package(405, pulsed + cw)

156505221

Obj. HCX PL APO63x/1.20 W CORR CS

0.14 – 0.1815506279

Page 27: TCS SP5 II System Overview - Leica Microsystems TCS SP5 II/Brochur… · System Overview TCS SP5 II Objectives w. M25 mount Optical outfit DM6000 CFS 1565006 12 IR Black Optical Table

27

SMD Filter cube

156505240156505241156505242156505243156505244156505245156505246156505247156505248156505249156505250156505251156505252

EmptyAlexa 405/cy2DAPI/Atto 647CFP/YFPCFP/RFPCy2/Cy5GFP narrow/RFPGFP wide/RFPFITC/TRITCGFP/HcRedAlexa 488/Alexa 633Neutral splittingPolarization splitting

Leica TCS SMD FLIM: FCS + External FLIM (PQ)

SMD detectionpackage external

FLIM + FCS(high time resolution,

2 MPD SPAD)156505206

IRLaser

Scanning stageincl. SuperZ

SuperZGalvo stage

X1 PortEmission access

156504500

Trigger unit156504760

UV/405 Port156501201 Correction lenses

SMD Excitationpackage

(405, pulsed + cw;470 +640 pulsed)

156505224

SMD Excitationpackage

(470 + 640, pulsed)156505222

SMD Excitationpackage

(470, pulsed)156505228

SMD Excitationpackage

(440 + 470, pulsed)156505230

SMD Excitationpackage

(440, pulsed)156505229

SMD Excitationpackage

(MP)156505225

System Overview TCS SP5 II

No

Yes

MP?

SP Channel 01 – 05(See page 6)

Basic Confocal ModuleAOBS

+ Basic Optical Module+ Imaging Laser AOBS

(See page 6)

SMD Excitation package(MP)

156505220

IR Imaging PortMultifunc.

(See page 12 ff.)

IR Beam Routing(See page 12 ff.)

IR Laser(See page 12 ff.)

IR black optical table(See page 12 ff.)

Notch filter set VISfor SP5

156504511

Notch filter set VISfor SP5

156504511

Notch filter set SMD156504509

Double filter wheel basicincl. analyzer158004505

Notch filter set SMD156504509

SMD Excitationpackage

(405 + 470 + 640, all pulsed)156505223

SMD Excitationpackage

(405, pulsed + cw)156505221

SMD Excitationpackage

(405, pulsed)156505220

Second license forSPT-1 + 2 (FCS + FLIM)

156505282

Please, order exactly one SMD detection and one SMD excitation package!Only exception: package 156505220 or 156505221 can be combined with 156505225.

Resonant scanning system 156501121 cannot be used for FLCS.

optional

Trigger unit156504760

Conventionalscanning system

156501120

Tandemscanning system

156501122

UV/405 Port156501201Correction lenses

SMD Excitation package(405, pulsed + cw)

156505221

Obj. HCX PL APO63x/1.20 W CORR CS

0.14 – 0.1815506279

Page 28: TCS SP5 II System Overview - Leica Microsystems TCS SP5 II/Brochur… · System Overview TCS SP5 II Objectives w. M25 mount Optical outfit DM6000 CFS 1565006 12 IR Black Optical Table

2828

SMD Filter cube

156505240156505241156505242156505243156505244156505245156505246156505247156505248156505249156505250156505251156505252

EmptyAlexa 405/cy2DAPI/Atto 647CFP/YFPCFP/RFPCy2/Cy5GFP narrow/RFPGFP wide/RFPFITC/TRITCGFP/HcRedAlexa 488/Alexa 633Neutral splittingPolarization splitting

Leica TCS SMD FLIM: FCS + External + Internal SP FLIM (PQ)

SMD detectionpackage external

FLIM + FCS(high time resolution,

2 MPD SPAD)+ spectral FLIM

(2 SP FLIM PMT)156505207

IRLaser

Scanning stageincl. SuperZ

SuperZGalvo stage

X1 PortEmission access

156504500

Trigger unit156504760

UV/405 Port156501201 Correction lenses

SMD Excitationpackage

(405, pulsed + cw;470 +640 pulsed)

156505224

SMD Excitationpackage

(470 + 640, pulsed)156505222

SMD Excitationpackage

(470, pulsed)156505228

SMD Excitationpackage

(440 + 470, pulsed)156505230

SMD Excitationpackage

(440, pulsed)156505229

SMD Excitationpackage

(MP)156505225

System Overview TCS SP5 II

No

Yes

MP?

SP Channel 01 – 05(See page 6)

Basic Confocal ModuleAOBS

+ Basic Optical Module+ Imaging Laser AOBS

(See page 6)

SMD Excitation package(MP)

156505220

IR Imaging PortMultifunc.

(See page 12 ff.)

IR Beam Routing(See page 12 ff.)

IR Laser(See page 12 ff.)

IR black optical table(See page 12 ff.)

Notch filter set VISfor SP5

156504511

Notch filter set VISfor SP5

156504511

Notch filter set SMD156504509

Double filter wheel basicincl. analyzer158004505

Notch filter set SMD156504509

SMD Excitationpackage

(405 + 470 + 640, all pulsed)156505223

SMD Excitationpackage

(405, pulsed + cw)156505221

SMD Excitationpackage

(405, pulsed)156505220

Second license forSPT-1 + 2 (FCS + FLIM)

156505282

Please, order exactly one SMD detection and one SMD excitation package!Only exception: package 156505220 or 156505221 can be combined with 156505225.

Resonant scanning system 156501121 cannot be used for FLCS.

optional

Trigger unit156504760

Conventionalscanning system

156501120

Tandemscanning system

156501122

UV/405 Port156501201Correction lenses

SMD Excitation package(405, pulsed + cw)

156505221

Obj. HCX PL APO63x/1.20 W CORR CS

0.14 – 0.1815506279

Page 29: TCS SP5 II System Overview - Leica Microsystems TCS SP5 II/Brochur… · System Overview TCS SP5 II Objectives w. M25 mount Optical outfit DM6000 CFS 1565006 12 IR Black Optical Table

29

SMD Filter cube

156505240156505241156505242156505243156505244156505245156505246156505247156505248156505249156505250156505251156505252

EmptyAlexa 405/cy2DAPI/Atto 647CFP/YFPCFP/RFPCy2/Cy5GFP narrow/RFPGFP wide/RFPFITC/TRITCGFP/HcRedAlexa 488/Alexa 633Neutral splittingPolarization splitting

Leica TCS SMD FLIM: FCS + External + Internal SP FLIM (PQ)

SMD detectionpackage external

FLIM + FCS(high time resolution,

2 MPD SPAD)+ spectral FLIM

(2 SP FLIM PMT)156505207

IRLaser

Scanning stageincl. SuperZ

SuperZGalvo stage

X1 PortEmission access

156504500

Trigger unit156504760

UV/405 Port156501201 Correction lenses

SMD Excitationpackage

(405, pulsed + cw;470 +640 pulsed)

156505224

SMD Excitationpackage

(470 + 640, pulsed)156505222

SMD Excitationpackage

(470, pulsed)156505228

SMD Excitationpackage

(440 + 470, pulsed)156505230

SMD Excitationpackage

(440, pulsed)156505229

SMD Excitationpackage

(MP)156505225

System Overview TCS SP5 II

No

Yes

MP?

SP Channel 01 – 05(See page 6)

Basic Confocal ModuleAOBS

+ Basic Optical Module+ Imaging Laser AOBS

(See page 6)

SMD Excitation package(MP)

156505220

IR Imaging PortMultifunc.

(See page 12 ff.)

IR Beam Routing(See page 12 ff.)

IR Laser(See page 12 ff.)

IR black optical table(See page 12 ff.)

Notch filter set VISfor SP5

156504511

Notch filter set VISfor SP5

156504511

Notch filter set SMD156504509

Double filter wheel basicincl. analyzer158004505

Notch filter set SMD156504509

SMD Excitationpackage

(405 + 470 + 640, all pulsed)156505223

SMD Excitationpackage

(405, pulsed + cw)156505221

SMD Excitationpackage

(405, pulsed)156505220

Second license forSPT-1 + 2 (FCS + FLIM)

156505282

Please, order exactly one SMD detection and one SMD excitation package!Only exception: package 156505220 or 156505221 can be combined with 156505225.

Resonant scanning system 156501121 cannot be used for FLCS.

optional

Trigger unit156504760

Conventionalscanning system

156501120

Tandemscanning system

156501122

UV/405 Port156501201Correction lenses

SMD Excitation package(405, pulsed + cw)

156505221

Obj. HCX PL APO63x/1.20 W CORR CS

0.14 – 0.1815506279

Page 30: TCS SP5 II System Overview - Leica Microsystems TCS SP5 II/Brochur… · System Overview TCS SP5 II Objectives w. M25 mount Optical outfit DM6000 CFS 1565006 12 IR Black Optical Table

www.leica-microsystems.com

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