System Microscope BX63/BX53 - Fluorescence ??System Microscope BX63/BX53 BX3 Series Photos courtesy of: Junko Kyozuka, ... BX61 Displacement Large Small BX63. 9 10 Motorized Fluorescence

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  • A Clear Edge in Imaging

    System Microscope

    BX63/BX53BX3 Series

    Photos courtesy of:Junko Kyozuka, Associate Professor, Graduate School of Agricultural and Life Sciences,

    The Tokyo University (P.11 below)Prof. Nakatani, Biochemistry course, School of medicine, Showa University (P.13 above)

    Prof. Tadokoro, Pathology class, School of medicine, St. Marianna University (P.15)

    Printed in Japan M1697E-122011

    OLYMPUS CORPORATION is ISO14001 certified. OLYMPUS CORPORATION is FM553994/ISO9001 certified. OLYMPUS CORPORATION is MD540624/ISO13485 certified. Illumination devices for microscope have suggested lifetimes. Periodic inspections are required. Please visit our web site for details. All company and product names are registered trademarks and/or trademarks of their respective owners. Images on the PC monitors are simulated. Specifications and appearances are subject to change without any notice or obligation on the part of the manufacturer.BX63/BX53 are the environmental conscious products according to OLYMPUS's own standards.

    Images are courtesy of:

    Dr. Shigeo Hayashi, Dr. Kagayaki Kato, Dr. Reiko Tajiri and Mr. Hosei WadaLaboratory for Morphogenetic SignalingRIKEN Center for Developmental Biology (P.12, P.15 top right, P.16 top left)

    Shigenobu Yonemura, Ph.D.Electron Microscope LaboratoryRIKEN Center for Developmental Biology (P.6 lower right, P.7, P.15 lower right, P.17 Phase contrast, P.19 top right)

    Guojun Sheng, Ph.D., Yukiko Nakaya, Ph.D.Laboratory for Early EmbryogenesisRIKEN Center for Developmental Biology (P.2, P.15 lower left, P.16 lower left)

    Fumio Matsuzaki, Ph.D., Daijiro Konno, Ph.D. Laboratory for Cell Asymmetry RIKEN Center for Developmental Biology (P.6 lower left, P.15 top left, P.16 top right)

    Junko Kyozuka, Associate ProfessorGraduate School of Agricultural and Life SciencesThe Tokyo University (P.16 lower right)

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    User-friendly design assures outstanding operating ease and flexibility forbrightfield/darkfield and fluorescence imaging in a wide range of researchsettings. With a choice of models and configuration options, there's anOlympus research microscope to meet your individual needs.

    A Revolutionary New Standard in Accuracy and Imaging Efficiency

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    Advanced Sensitivity in Fluorescence Imaging

    Motorized Sensors with Power-offFunctionsThe sensor for the motorized fluorescenceilluminator BX3-RFAA turns the light on onlyupon mirror unit changeover commands,and turns it off as soon as the command iscompleted. This function assures that straylight from the sensor is terminated duringobservation.

    New Fluorescence Illuminators with Flys-eye LensFluorescence illuminators BX3-RFAA andBX3-RFAS equipped with a Flys-eye lensprovide uniform illumination. Even, uniformfluorescence illumination facilitates post-imaging enhancements and processing.

    Accuracy & Ease of Use

    High Transmission Objectives withReduced AutofluorescenceOlympus UIS2 objectives are made withsuperior low-autofluorescence glass, anti-reflection coats, and lens joining materials,improving the S/N ratio. Effective detectionsof subtle fluorescence emissions even withweak excitation light deliver idealperformance in fluorescence imaging.

    Condenser Design to Reduce Back-reflectionsThe motorized universal condenser isdesigned to reduce back-reflections andautofluorescence by swinging its top lensout, automatically closing its diaphragm tothe minimum, and locating the wheel inbetween two positions.

    Low Autofluorescence Immersion OilThis immersion oil isspecifically designedto reduce theautofluorescencenormally associatedwith such oils, for

    improved S/N ratio. Insusceptible tocrystallization, it is an ideal oil for long timeobservations.

    Fluorescent Mirror Units with the Latest Coating TechnologyOlympus applies an outstanding filtercoating technology to all fluorescencemirror units in order to produce hightransmissions, sharp cut-offs and efficientdetections of fluorescence.

    Stray Light Reduction Equipped on All Mirror UnitsThe low reflection function eliminates over99% of stray light, producing a high S/Nratio.

    Low reflection function

    Stray light

    Excitation filter

    Emission filter

    Fluorescence light for observation



    Excitation light: Illumination light

    Dichromatic mirror

    Light source

    High-sensitivity fluorescence detection enables bright, high-contrast imagingwith minimal exposure of cells to excitation light. Advanced Olympustechnologies also reduce stray light and autofluorescence to assure a high S/N ratio.






    500 550Wavelength (nm)





    600 650 700 750 800























    300 350 400 450 500 550Wavelength (nm)



    AccuracySurface of Flys-eye Lens,

    Enlarged Image

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    Accuracy & Ease of Use Reliability and Precision in an Easy-to-use Package

    Effortless Imaging The cellSens software allows researchers toperform effortless imaging.

    NRK-52E cells (Alexa Fluor 488/Alexa Fluor 546)Brain section of mouse at embryonic day 15 (Cy3)

    Further Fluency in FluorescenceIntegration The Olympus 8-position fluorescenceilluminator allows flexible responses tovarious fluorescence specimens, witheasily replaceable mirror units.Observations are acceleratedfurther with less need forchanging mirror units forspecimens such as multi-colors and FISH.

    Upgraded AccuracyThe BX63 microscope features a fixed stage and focusingnosepiece for maximum stability and accurate imaging. An ultrasonic drive system enables fine, smooth control of specimen position.

    Accuracy Ease of Use


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    BX63 Intelligent Design for MaximumConfiguration Flexibility and Operating EaseThe BX63 offers outstanding stability and imaging precision for researchapplications, with the convenience of a touch screen interface for easyoperation. Additionally, it features a detachable controller that can bepositioned to suit the operator's preference or workflow requirements.

    Programmable controls greatly simplify repetitive observation and imaging tasks.In Navigation mode, the interface is context-sensitive, and displays only the functionsthat are relevant to the currently selected observation method.In Full Operation mode, it allows researchers access to the full range of functions andcustomization options available.

    The Olympus BX63 is the choice for todays motorized microscope.By incorporating a motorized nosepiece and securely fastening the stage on threepoints, the microscope is even more stabilized. The smooth, high-precision drive of the fixed ultrasonic stage reduces vibrations, further advancing the quality of image acquisition.

    Intuitive Touch Panel Controller for Enhanced Workspace andWorkflow Flexibility

    Stability for Improved Imaging Reliability

    The Detachable Controller Customizes Workspace into an Efficient Microscope Workstation

    The controller U-MCZ can be removed and attached anywhere on the microscope,enabling the researcher to create a working environment all his/her own. The XY-controller of the ultrasonic stage can also be attached to the users preferredside, allowing the simultaneous operation of the controller and the mouse.The controllers functionally-placed switches simplify observation method/objective/mirror unit changeovers, light intensity adjustments and live/archive image selections.

    Accuracy & Ease of Use BX63

    Intelligent Microscope BX63 + Digital Camera DP73

    Touch Panel Controller

    Stability Simulation in Comparison to BX61

    U-MCZ+BX3-SSU Ultrasonic Stage XY-controller





    Small BX63

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    Motorized Fluorescence Illuminator/BX3-RFAAThe flexibility of the motorized fluorescenceilluminator accommodates multi-colorstained specimens. The 8-position mirror units permit quickchangeover of fluorescence colors.

    Motorized Attenuator Wheel/U-AWThe Olympus BX63 has a motorized NDfilter wheel for fluorescence and transmittedlight intensity adjustments.* Special adapters are required for mounting (U-LHEAD for fluorescence, and U-LH100ADP for transmitted light).

    Touch Panel Controller


    Software cellSens

    Motorized Seven Position Nosepiece/U-D7REAEquipped with a DIC slider slot, thisrevolving nosepiece allows simultaneousattachment of seven objectives. It isespecially suitable for continuousobservations from low to high magnificationsand combining specific objectives, such aspolarized light observations.

    Motorized Units Facilitate Operations

    Scanning Stage with Ultrasonic/BX3-SSUThe ultrasonic stage delivers high-precisionXY control. The XY-controller can bemounted on the controller/U-MCZ for theBX63 and worked like conventional stagehandles.

    Motorized Universal Condenser/BX3-UCD8ABy integrating with designated opticalcomponents, the motorized universalcondenser accommodates various kinds oftransmitted light observation, from brightfieldto differential interference contrast andphase contrast.

    With the BX63, a diverse lineup of motorized units are available.The researcher has a choice of three controlsthe touch panelcontroller, the controller/U-MCZ, or the imaging softwarecellSensfor