Page 1Supplementary data

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Leaf 4 Leaf 7 Leaf 4 Leaf 7 Leaf 4 Leaf 7 Leaf 4 Leaf 7 Leaf 4 Leaf 7 Leaf 4 Leaf7 Leaf 4 Leaf7 Leaf 4 Leaf7

Wild-type SALK 113361 35S::GTE6

Line1

35S::GTE6

Line2

35S::GTE6

Line3

GTE6 RNAi

Line 1

GTE6 RNAi

Line 2

GTE6 RNAi

Line 3

Wid

th/L

en

gth

ra

tios

35S::GTE6C

WT

line

1

line

2

35S::GTE6

TPC2

ACT8

E

WT

line

1

line

2

35S::GTE6

AN

ACT8

A

WT

line

1

line

2lin

e 4

35S::GTE6

ROT3

ACT8

D

WT

line

1

line

2

35S::GTE6B

WT

line

1

line

2

Figure S2. Expression of regulatory genes in 35S::GTE6 plants.RT-PCR analysis of transcript abundance of (A) AN, (B) CLF, (C) DRL, (D) ROT3 and (E) TPC2 in low (line 2), medium (line 1) and high (line 4) expressing lines of 35S::GTE6 plants, using ACT8 as an internal control.

line

4lin

e 4

line

4

line

4

CLF

ACT8DRL

ACT8

Figure S1. Width/length ratios of leaf lamina.The width to length ratios of leaf lamina of leaves 4 and 7 of wild-type Col, Salk_113361, 35S::GTE6 and GTE6 RNAi plants. Error bars indicate standard error of the mean. N=5.

ET_domain YD KR IN LP KL R II P S E E D TL T L Yeast_BDF1 NKLKTVVTYDMKRIITERINDLPTSKLERAIDIIKKSMPNI--SEDDEVELDLDTLDNHTILTLYNTFFRQHuman_RING3 EEESRPMSYDEKRQLSLDINKLPGEKLGRVVHIIQAREPSLRDSNPEEIEIDFETLKPSTLRELERYVLSCHuman_Orfx EEEGLPMSYDEKRQLSLDINRLPGEKLGRVVHIIQSREPSLRDSNPDEIEIDFETLKPTTLRELERYVKSCDroso_Fsh EDTAKPMSYDEKRQLSLDINKLPGDKLGRVVHIIQNREPSLRDSNPDEIEIDFETLKPSTLRELESYVASCArab_GTE6 VERCRKITIEEKRNIGLALLKLSPDDLQKVLGIVAQANPSFQ-PRAEEVSIEMDILDEPTLWRLKFFVKDA

ET_domain G K Q Yeast_BDF1 YESSSGASNGLDGTSGVTRDASSLSPTSAGSRKRRSKALSQEEQSRQIEKIKNKLAILDSASPLSQNGSPGHuman_RING3 LRKKPRKPYTIKKPVGKTKEELALE-KKRELEKRLQDVSGQLNSTKKPPKKANEKTESSSAQQVAVSRLSAHuman_Orfx LQKKQRKPFS---ASGKKQAAKSKE-ELAQEKKKELEKRLQDVSGQLSSSKKPARKEKPGSAPSGGPSRLSDroso_Fsh LRKKTRKPYYKKKPSGKSKDEQMAE-KKQELEKRLQDVTGQLGASKKTAKKDESASSKVEAVQPANPVSSSArab_GTE6 LDNAM-----KKKKEEETKTRELSGAQKKEVSKK-RNATTKL-AERKTKRSRI------------------

ET_domain S SS S S SYeast_BDF1 QIQSAAHNGFSSSSDDDVSSESEEEHuman_RING3 SSSSSDSSSSSSSSSSSDTSDSDSGHuman_Orfx SSSSSESGSSSSSGSSSDSSDSE–Droso_Fsh SSSSDSSSSSSSDSSSSDSSDSEAGArab_GTE6 -------------------------

Figure S3. Alignment of ET domains.Alignment was performed using PileUp in the GCG package (Wisconsin Package Version 10.3, Accelrys Inc., USA). Accession numbers: Yeast BDF, Z18944; Human Ring3, P25440; Human Orfx, D26362; Drosophila Fsh P13709; Arabidopsis GTE6, NP190796.

Supplementary data Page 2

Table 1. Sequence of primers.

GTE6raceR4, 5'-AAATCTGCGAAAGACTGGAATGAGT,GTE6raceR5, 5'-CTGAGTCAGCCATTCACAGCTTCTC,GTE6raceR6, 5'-CGTCGACACGCTGCTTGATGCACTC,LBB1, TTTTTCGCCCTTTGACGTTGGAGT, GTE6p-F2, TGGTTTTAAGAAGTTTATGGTGTGGT, GTE6p-F3, AAGATATATCTATATTTGCTGGCTTG, GTE6p- R2, GACATGACCTGGCACTGAGTC,AS1-f, 5'-GAAAGAGAGACAACGTTGGAGTGGT,AS1-r, 5'-GGGCGGTCTAATCTGCAACCCATT,ACT8 forward primer, 5'-TAAACTAAAGAGACATCGTTTCCA,ACT8 reverse primer, 5'-TTTTTATCCGAGTTTGAAGAGGC,AS1 forward primer, 5'-AGCCTTCTTAGCCTCCATGC,AS1 reverse primer, 5'-AAGATTGCTGCTGAGGTTCC,GTE6 forward primer, 5'-CAATGCCAATCCAATATTACG,GTE6 reverse primer, 5'-CAATGCCAATCCAATATTACG,AN forward primer, 5'-ACGGTTCAGATACTCAATGCAG,AN reverse primer, 5'-CTACGTACGATCTCAGTGCTGG,CLF forward primer, 5'-AGAAGATGGACCTGCCAGTTC,CLF reverse primer, 5'-GCTTGATTATCGCCACTCTCG,ROT3 forward primer, 5'-CATGGCTCTGGTTACGTCTACG,ROT3 reverse primer, 5'-AGGTGAGGAGATCTGAGGAACG,AS2 forward primer, 5'-AACGTGACAAAGCTCCTCAACG,AS2 reverse primer, 5'-TGCTGAAACTGAGTGATCTGTCC,DRL forward primer, 5'-TAGGCAATGGAATAAGGAACGG,DRL reverse primer, 5'-TGTCTGCATCAGTTGGTAACGG,TCP2 forward primer, 5'-TGTCCGGTTATCAGTCTCCAC,TCP2 reverse primer, 5'-CTTGGTGCTGATGATGAGGAT,18S rDNA forward, 5'-GGTGGTGGTGCATGGCCGTTCTT,18S rDNA reverse, 5'-GACGGGCGGTGTGTACAAAGGGC,Region P1 of AS1:as1p1F, 5'-CCTGTTTGGTTTCCAGAATATAGGT,

as1p1R, 5'-GTTTCTCCTGAAGACGGATCACAAG.Region P2 of AS1:as1p2F, 5'-GATCATCATTGCGAAATCTTCC,

as1p2R, 5'-TACACGATTAGACCACAGAGACG.Region P3 of AS1:as1p3F, 5'-AATGCTGTGTCTGCTACGATTAAC,

as1p3R 5'-GGATTTGACGTATGAGTAATTGGG.Region P0 of AS1: AS1 forward primer, 5'-AGCCTTCTTAGCCTCCATGC,

AS1 reverse primer, 5'-AAGATTGCTGCTGAGGTTCC.

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