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Strongiloides stercolaris in bronchioloalveolar lavage 1 MedDocs Publishers Received: Apr 06, 2020 Accepted: May 11, 2020 Published Online: May 13, 2020 Journal: Journal of Clinical Images Publisher: MedDocs Publishers LLC Online edion: hp://meddocsonline.org/ Copyright: © Palaoro L (2020). This Arcle is distributed under the terms of Creave Commons Aribuon 4.0 Internaonal License *Corresponding Author(s): Luis Palaoro Department of clinical Biochemistry, clinical Hospital (UBA), cordoba 2351 (1120) C.A.B.A.-INFIBIOC, Argenna. Tel: 5411 4552 0449; Email: [email protected] Journal of Clinical Images Open Access | Clinical Image Cite this arcle: Rocher A, Guerra F, Gimenez CL, Palaoro L. Strongiloides stercolaris in bronchioloalveolar lavage. J Clin Images. 2020; 3(1): 1038. Clinical image descripon A 57-year-old male paent, former smoker, nave of Mi- siones (Argenna), was presented to the Emergency Depart- ment of the Clinical Hospital of Buenos Aires, Argenna, with a worsening oxygen requirement, chronic cough, shortness of breath and voming of 1 week of evoluon. He suffered from stage 3-4 squamous cell carcinoma of the tonsil since 2 years, treated with chemotherapy with paclitaxel, 5FU, cisplan). Chestradiography was performed, which showed diffuse bi- lateral lung consolidaon. Bronchioalveolar lavage (BAL) was performed to evaluate diffuse infiltrates in alveoli and lung in- tersces. The cytological examinaon of the material obtained- Keywords: Bronchoalveolar lavage (BAL); Convenonal cytol- ogy; Immunosuppression; Strongiloidesstercolaris. Adriana Rocher; Fernando Guerra; Cina Lorena Gimenez; Luis Palaoro* Department of clinical Biochemistry, clinical Hospital (UBA), cordoba 2351 (1120) C.A.B.A.-INFIBIOC – Argenna. showed Strongiloides stercolaris filariform larvae in fresh and in stained samples with the Giemsa and Papanicolaou methods. Giemsa’smethod, simple and fast, was to be very useful for the cytodiagnosis of this parasitosis. So far as we know, this is the first report of this method for the observaon of Strongiloides stercolaris larvae in BAL [1] (Figures 1-3). Strongyloidosis is an endemic helminthiasis of Southwest Asia, Africa, and South America [2]. Severely immunosuppressed paents, with hyperinfecon, usually present the parasite in the lungs, since the larvae can evolve into filariform forms before being excreted in the faeces, with the ability to spread to other sites. Our paent presented not only the larvae in the BAL, but also the parasite’s eggs, which is an exceponal fact [3].

Strongiloides stercolaris in bronchioloalveolar lavage...1. Zhao P, Maleki Z. Strongyloides stercoralis in bronchoalveolar lavage specimen processed as Prussian Blue Stain. Diagn Cyto-pathol

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Page 1: Strongiloides stercolaris in bronchioloalveolar lavage...1. Zhao P, Maleki Z. Strongyloides stercoralis in bronchoalveolar lavage specimen processed as Prussian Blue Stain. Diagn Cyto-pathol

Strongiloides stercolaris in bronchioloalveolar lavage

1

MedDocs Publishers

Received: Apr 06, 2020Accepted: May 11, 2020Published Online: May 13, 2020Journal: Journal of Clinical ImagesPublisher: MedDocs Publishers LLCOnline edition: http://meddocsonline.org/Copyright: © Palaoro L (2020). This Article is distributed under the terms of Creative Commons Attribution 4.0 International License

*Corresponding Author(s): Luis PalaoroDepartment of clinical Biochemistry, clinical Hospital (UBA), cordoba 2351 (1120) C.A.B.A.-INFIBIOC, Argentina.Tel: 5411 4552 0449; Email: [email protected]

Journal of Clinical Images

Open Access | Clinical Image

Cite this article: Rocher A, Guerra F, Gimenez CL, Palaoro L. Strongiloides stercolaris in bronchioloalveolar lavage. J Clin Images. 2020; 3(1): 1038.

Clinical image description

A 57-year-old male patient, former smoker, native of Mi-siones (Argentina), was presented to the Emergency Depart-ment of the Clinical Hospital of Buenos Aires, Argentina, with a worsening oxygen requirement, chronic cough, shortness of breath and vomiting of 1 week of evolution. He suffered from stage 3-4 squamous cell carcinoma of the tonsil since 2 years, treated with chemotherapy with paclitaxel, 5FU, cisplatin).

Chestradiography was performed, which showed diffuse bi-lateral lung consolidation. Bronchioalveolar lavage (BAL) was performed to evaluate diffuse infiltrates in alveoli and lung in-terstices. The cytological examination of the material obtained-

Keywords: Bronchoalveolar lavage (BAL); Conventional cytol-ogy; Immunosuppression; Strongiloidesstercolaris.

Adriana Rocher; Fernando Guerra; Cintia Lorena Gimenez; Luis Palaoro*Department of clinical Biochemistry, clinical Hospital (UBA), cordoba 2351 (1120) C.A.B.A.-INFIBIOC – Argentina.

showed Strongiloides stercolaris filariform larvae in fresh and in stained samples with the Giemsa and Papanicolaou methods.Giemsa’smethod, simple and fast, was to be very useful for the cytodiagnosis of this parasitosis. So far as we know, this is the first report of this method for the observation of Strongiloides stercolaris larvae in BAL [1] (Figures 1-3).

Strongyloidosis is an endemic helminthiasis of Southwest Asia, Africa, and South America [2]. Severely immunosuppressed patients, with hyperinfection, usually present the parasite in the lungs, since the larvae can evolve into filariform forms before being excreted in the faeces, with the ability to spread to other sites. Our patient presented not only the larvae in the BAL, but also the parasite’s eggs, which is an exceptional fact [3].

Page 2: Strongiloides stercolaris in bronchioloalveolar lavage...1. Zhao P, Maleki Z. Strongyloides stercoralis in bronchoalveolar lavage specimen processed as Prussian Blue Stain. Diagn Cyto-pathol

The cytological and histological examination of the affected tissues is the method of choice due to its great sensitivity and specificity, for cases of Strongyloides stercolaris hyperinfection. Pulmonary strongyloidiasis should be considered as a possible differential diagnosis if alveolar and interstitial shadow patterns are observed on chest radiographs of patients with immunode-ficiencies [4].

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MedDocs Publishers

Journal of Clinical Images

References

Zhao P, Maleki Z. Strongyloides stercoralis in bronchoalveolar 1. lavage specimen processed as Prussian Blue Stain. Diagn Cyto-pathol. 2013; 41: 141-143. doi: 10.1002/dc.21822. Epub 2011 Sep 30.

Schär F, Trostdorf U, Giardina F, Khieu V, Muth S, et. al. Strongy-2. loides stercoralis: Global Distribution and Risk Factors. PLoS Negl Trop Dis. 2013; 7: e2288.

Bava AJ, Troncoso AR. Strongyloides stercoralis hyper¬infection 3. in a patient with AIDS. J Int Assoc Physicians AIDS Care (Chic) 2009; 8: 235-238

Requena A, Chiodini P, Bisoffi Z, Buonfrate D, Gotuzzo E et al. 4. The laboratory diagnosis and follow up of strongyloidiasis: a sys-tematic review. PLOS Negl Trop Dis. 2013; 7: e2002.

Figure 1: Filariform larva of Strongyloides stercoralis in BAL (Gi-emsa stain-800x)

Figure 2: Strongyloides stercoralis egg in BAL (Giemsa stain-800x)

Figure 3: Filariform larva of Strongyloides stercoralis in BAL (Pap stain-800x)