Solutions for Biopharmaceuticals Analysis .Biopharmaceuticals Analysis ... Uniform graphical user

  • View
    219

  • Download
    1

Embed Size (px)

Text of Solutions for Biopharmaceuticals Analysis .Biopharmaceuticals Analysis ... Uniform graphical user

  • Solutions for Biopharmaceuticals Analysis

    C10G-E039

  • Shimadzus innovative and robust instruments to accelerate your workflow !

    Discovery

    Perfinity iDP

    Integrated Protein Digestion HPLC

    MALDI-7090

    High-performance MALDI TOF-TOF Mass Spectrometer

    Research

    Shimadzu is an industry leader in providing innovative analytical solutions for the biopharmaceutical and

    pharmaceutical market segments. Working closely with global collaborators and partners in industry and

    academia, Shimadzu develops products that meet customer expectations for robustness, reproducibility, and

    versatility. You can depend on Shimadzus extensive range of analytical products to help you in your daily

    laboratory workflows in Drug Discovery and Development, Clinical Trials, and QA/QC.

  • Development

    Clinical Trials

    Production Quality Assurance

    Quantitative Proteomics

    LCMS-8050 with Skyline

    Co-sense BA

    Automated SamplePre-treatment

    ProteinSSSSSSequencer

    Edman Sequencing

    PPSQ Series

  • 4

    The MALDI-7090 sets a new standard in MS/MS acquisition. Several novel and exclusive technologies have been combined to create Hyper-MS2.

    The MALDI-7090 is equipped with a dual wire-grid high-resolution ion gate.Compounds of similar nominal mass may produce MS/MS spectra that contain fragment ions from several precursors if not gated correctly. However, the high-resolution ion gate in the MALDI-7090 allows the individual gating of species close in nominal mass, thus producing distinct fragment ion spectra.

    High-resolution ion gate

    Ultimate performance in identification and structural characterization of biomolecules.

    0

    10

    20

    30

    40

    50

    60

    70

    80

    90

    100

    m/z860 870 880 890 900 910 920 930 940 950 960 970 980

    0

    10

    20

    30

    40

    50

    60

    70

    80

    90

    100

    m/z860 870 880 890 900 910 920 930 940 950 960 970 980

    0

    10

    20

    30

    40

    50

    60

    70

    80

    90

    100

    m/z1030 1032 1034 1036 1038 1040 1042 1044 1046 1048 1050 1052 1054 1056 1058 1060 1062 1064 1066 1068 1070

    MS/MSlow-resolution ion gatepeptide A + peptide B

    MS/MS high-resolution ion gate

    peptide A only

    y7 peptide A

    (b-H2O)7 peptide A

    b7 peptide A

    y7 peptide A

    (b-H2O)7 peptide A

    b7 peptide A

    y7 peptide B

    (b-H2O)7 peptide B

    b7 peptide B

    peptide A

    peptide B

    Features of MALDI-7090

    Ultrafastacquisition

    speedMALDI-7090

    UltimateMS/MS

    resolution

    Biiioooomolleeecules Research

  • 5

    The result of applying ASDF in addition to pulsed extraction during an MS/MS acquisition is illustrated below. The full MS/MS spectrum shown in the inset demonstrates almost complete sequence coverage of the detected fragment ions. The detailed region (m/z 1190 - 1310) shows the high resolution achieved using ASDF (10000 FWHM) as well as the presence of high-energy w-type fragment ions characteristic of side chain fragmentation.

    551.55

    573.54

    645.48

    829.73

    x20

    100

    80

    60

    40

    20

    0680 700 720 740 760 775

    O

    O

    OOO

    O

    %In

    ten

    sity

    100

    90

    80

    70

    60

    50

    40

    30

    20

    10

    0500 540 580 620 660 700 740 780 820 860

    m/z

    High-energy CIDfragment ions

    1807 1808 1809m/z

    1810 1811

    NTPpSQHDHpSIQHSPER

    (M+H)+Neutral loss of phosphoric acid

    1780 1800 1820 1840 1860 1880 1900 1920 1940 1960 1980 2000 2020

    m/z

    %In

    ten

    sity

    100

    90

    80

    70

    60

    50

    40

    30

    20

    10

    0

    1808.156

    1807.126

    1905.063

    1790.125

    1789.106

    1805.0081843.089 1863.127 1887.964 1923.089 1954.304

    1993.979

    1985.792

    (98 Da)Neutral loss of phosphoric acid(98 Da)

    ASDF is a Shimadzu patented technology that enables unparalleled resolution in MS/MS acquisitions. Through correction of the axial spatial distribution of the ions generated, the mass resolution is significantly increased and becomes essentially independent of the laser power used to ionize the sample.With ASDF, the MALDI-7090 can achieve mass resolution of 10,000 FHWM unobtainable through pulsed extraction and ion optics alone.

    Axial Spatial Distribution Focusing - ASDF

  • 6

    The Perfinity iDP (Integrated Digestion Platform) system digests proteins using a dedicated trypsin column. The peptide fragments obtained from digestion are separated in a reverse-phase column using HPLC. By fully automating the series of steps, the system is able to significantly reduce the time required for analysis. By linking directly to an LC/MS system, the resulting peptide fragments can be identified automatically online. Compared to manual methods, fully automating the process minimizes human error and provides reproducible results.

    Rapid online trypsin digestion using a high-efficiency trypsin column Dedicated software supports methods that extend from pretreatment to analysis Automatic online analysis results in high reproducibility and reliability Online connectivity to mass spectrometers provides broad applicability

    Perfinity iDP

    Manual Method

    Protein digestion 18-24 hours HPLC or LC/MSanalysisSolid phaseextraction

    Re-suspension

    HPLC or LC/MSanalysisDesalting

    Proteindigestion

    (1-4 minutes)

    Perfinity iDP reduces the entire sample preparation

    workflow down to minutes20

    Reduce trypsin digestion time to

    minutes1-4

    Perfinity iDP

    PPeeppttiiddee MMaappppiinngg

    Peptide mapping is an essential analytical approach to confirm amino acid sequences and any modifications. This is useful for characterization and QC of biopharmaceuticals as well as in research fields.The reproducibility of the analysis is important to compare chromatograms.

    Rapid on-line trypsin digestion

    Features of Perfinity iDP

  • 7

    Highly Reproducible Peptide Mapping

    LC Systems

    Maximum Reliability and

    Stability

    ExceptionalOperational

    Efficiency

    MaximumEasy-of-use

    Nexera-i

    Uniform graphical user interfaces between the system and workstation allow intuitive operations regardless of experience level and increase the operation availability of the i-Series. The browser functions in LabSolutions bring rapid processing of large amounts of data, real-time statistical calculation and easy confirmation of anomalous values, enabling more efficient data processing.The i-Series saves lab operators time and energy. Combined with the LabSolutions automated functions, it reliably completes analyses under specified procedures.

    Features of Nexera-i Series

    Intra-Day Repeatability for Chromatograms of IgG Tryptic Digests

  • Sensitivitydown to lowpicomole level

    Worlds onlyautomated Edman

    degradationsequencer

    Stableretention timesand baselines

    Differentiationof isobaric

    amino acids PPSQ Series

    AAccccuurraattee PProoteeiinn SSeeqquueennccee DDeetteermiinnaattiioonn

    N-terminal amino acid analysis is essential to confirm the type and uniformity of N-terminal amino acids. This analysis employs the Edman method (sequential cleaving of amino acids from the N-terminal of the protein to determine the amino acid sequence), which is the most reliable method available for determining amino acid sequences.The PPSQ Protein Sequencer Systems automate the Edman reaction, LC separation, detection and data analysis to determine the amino acid sequence from the N-terminal.

    Protein sequencer

    8

    1. Easy-to-use and cost-effective solution. 2. Easy data analysis assisted. 3. Shimadzu provides full support.

    Features of PPSQ Series

    Thr (cycle 5)

    Leu (cycle 2)

    Met (cycle 4)

    Val (cycle 3)

  • Chromatograms of sequence analysis of IgGmouse) light chain

    9

    The results show the N-terminus of the light chain is uniform and the identified amino sequence is Asp-Leu-Val-Met-Thr from N-terminus.

    Data

    Analysis of Commercial Lab Mouse Monoclonal Antibodies

    Heavy chain

    SDS-PAGE Electro-blotting PPSQ

    Light chain

    Asp (cycle 1)

    Thr (cycle 5)Thr (cycle 5)

    DTT DMPTU

    DPU

    DPTU

    Leu (cycle 2)Leu (cycle 2)

    Met (cycle 4)Met (cycle 4)

    Val (cycle 3)Val (cycle 3)

    Heavy and light chains subject to SDS-PAGE are electroblotted onto PVDF membranes for sequencing.

  • 10

    Combinationwith

    SkylineUltrafast

    MRM

    Shimadzus LCMS-8050 is the fastest triple quad on the market. Combination with the Skyline environmental significantly increases the throughput of quantitative proteomics.

    LCMS-8050

    Predict digest peptides

    Calculate precursor ions for each valence state

    Select product ion candidates

    (using measured or calculated product ions)

    Select transitions

    Calculated retention time values

    Calculated CE values

    Select peptides for quantitation

    Select product ions

    Evaluate results from considering CEs

    0.0

    (10,000,000)

    2.00

    1.75

    1.50

    1.25

    1.00

    0.75

    0.50

    0.25

    0.00

    1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 min

    1.5 2.0 2.5 1.5 3.5 4.0 4.5 5.0 5.5 6.0 6.5 7.0 min

    2.00

    1.75

    1.50

    1.25

    1.00

    0.75

    0.50

    0.25

    0.00

    (1,000,000)

    (1,000,000)2.00

    1.75

    1.50

    1.25

    1.00

    0.7