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Scientific Service Bacterial Diseases
NRC Listeria
Recent trends of listeriosis in Belgium
Sophie Bertrand, Pieter-Jan Ceyssens, Jean Vanderpas, Raymond Vanhoof and
Wesley Mattheus
May 19th 2016
Rue Juliette Wytsmanstraat 14 | 1050 Brussels | Belgium
T +32 2 642 51 11 | F +32 2 642 50 01 | email: [email protected] | www.wiv-isp.be
OD-Communicable and infectious diseases
1: What is a Listeriosis?
Invasive disease due to a Listeria monocytogenes infection
Population at risk (pregnant women, elderly
or immunocompromised)
Mortality reaching up to 20-30%
OD-Communicable and infectious diseases
1: Listeria: 6-7 species
L. monocytogenes
L. ivanovii
L. innocua
L. grayi
L. seeligeri
L. welshimeri
+ L. marrayii?
1: Listeria monocytogenes
• Classical typing: 13 serotypes
OD-Communicable and infectious diseases
1: Sources of contamination
• Widespread in the environment
• Resistant: T between 0ºC - 50ºC
• Zoonosis
OD-Communicable and infectious diseases
1:Sources of contamination
Products with raw milk
Salmon/ Sliced fish
Prepared dishes
Incubation period:
few days to 3 months
OD-Communicable and infectious diseases
1:European Directive
Directive EC Nº2073/2005 (Nov 2005)
• Absence in 25g (products for new-born and therapeutic purposes)
• Absence in 25g (In products that enable the development of LM) unless the product meets the limit of 100 LM/gr
• In products that do not allow the development of LM: 100 LM limit/gr
OD-Communicable and infectious diseases
2: National Reference Centre of Listeria
• Exists since 1966, data collected since 1985, Officially recognised by
INAMI in 2010
2015:
• Human isolates:160 from clinical laboratories
• Food isolates : AFSCA + + Food industries
Serotyping for confirmation of the diagnosis, identification of the source of infection, control of the products and a better understanding of the epidemiology of Listeria.
ACCREDITATION BELTEST NORME NBN EN ISO 17025 CERTIFICAT NR: 109-T
OD-Communicable and infectious diseases
3:Trends: incidence 2015 = 0.67; 2014 = 0.72
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OD-Communicable and infectious diseases
3:Trends: retrospective study
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OD-Communicable and infectious diseases
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OD-Communicable and infectious diseases
3:Trends: retrospective study
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Incidence Incidence n-MN Incidence MN
Linear (Incidence n-MN) Linear (Incidence MN)
OD-Communicable and infectious diseases
3:Trends: retrospective study
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serovar 4b serovar 1/2a Linear (serovar 1/2a)
OD-Communicable and infectious diseases
3:Trends: retrospective study
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0-9 10-19 20-29 30-39 40-49 50-59 60-69 70-79 80-89 >90 y
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OD-Communicable and infectious diseases
3:Trends: retrospective study
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inh
ab
Females Males Total
OD-Communicable and infectious diseases
4: Listeriosis and associated pathologies
%N: Relative % to the number of cases with a complementary pathology %n: Relative % to the number of cases with an indication
N %n %N
Cancer 185 43.1 23.7
Digestive diseases 52 12.1 6.6
Respiratory diseases 35 8.2 4.5
Chronic kidney diseases 33 7.7 4.2
Immunosuppression 29 6.8 3.7
Heart diseases 23 5.4 2.9
Alcoholism 20 4.7 2.6
Iatrogenic immunosuppression 20 4.7 2.6
Transplantation 14 3.3 1.8
Surgery 12 2.8 1.5
Autoimmune Diseases 2 0.5 0.3
infection/wound 1 0.2 0.1
No indication 359 45.9
Total 785
OD-Communicable and infectious diseases
4: Listeriosis and associated pathologies
N %n
Haematological 75 40.5
Digestive tract 25 13.5
Lung 21 11.4
Breast 12 6.5
Others 16 8.6
Unknown 36 19.5
Total 185 100
OD-Communicable and infectious diseases
5: Listeriosis outbreak 2011
• Alert Epis (ECDC) 05/04/2011
• One reaction from France
• Rapid Alert System for Food and Feed (RASFF) of the European Commission
• Contamination of Hard Cheese (<100 LM/g)
• Removing cheese (national and international)
OD-Communicable and infectious diseases
6. Conclusions Listeriosis: rare disease but severe
Global incidence of Listeriosis increases
The Materno-fetal incidence is decreasing
Increase of serovar 1/2a
Population at risks:
Man up to 60y old
Blood cancer
Susceptible to low level of contamination
OD-Communicable and infectious diseases
Thanks
Dr. M. Yde
Dr. J. Vanderpas
Dr. R. Vanhoof
Dr. P-J Ceyssens
Dr. W. Mattheus
Thank you for your attention!
Questions?
