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Science Investigatory Project

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A science investigatory project conducted at BNHS-RSHS for Region IV-B MIMAROPA, Philippines.

Text of Science Investigatory Project


IV. Research PlanStatement of the ProblemThis study will be conducted to test the capability of aloe gel (Aloe barbadensis), honey (Honey) and guava (Psidium guajava) leaf extracts in lightening scars. It will be then tested to see if it can answer the following questions:1) What are the properties of aloe (Aloe barbadensis) gel, honey (Honey) and guava (Psidium guajava) leaf extracts that help lighten scars?2) Is there any significant difference between the experimental product and the commercial product in terms of:2.1) Odor2.2) Sensation after applying2.3) Scar appearance after 2 weeksNull HypothesesIt will be hypothesized that:1) Aloe gel, honey and guava leaf extract has no properties that can help lighten scars.2) There is no significant difference between the experimental product and the commercial product in terms of:2.1) Odor2.2) Sensation after applying2.3) Scar appearance after 2 weeksMaterials and EquipmentThe materials to be used in the study are: one triple beam balance, one stirring rod, two beakers, 3g aloe, 3g honey, 3g guava leaf extracts, 0.40g xanthan gum, 0.30g ethylenediamenetetraacetic acid, 2.0g triethanolamine , 3.0g cetyl alcohol, 3.5g stearic acid, 3.3g glycerylmonestearate, 3.5g mineral oil, and 1.2g isopropyl myristate.General ProcedureIn preparation of the raw materials, aloe leaves will be gathered, blanched, blended, and filtered. Then the aloe will be weighed using a triple beam balance to satisfy the amount needed (3g) in producing the product. Then three (3g) of natural honey will also be gathered by weighing the honey using the triple beam balance. And lastly, 139g of guava leaves are to be gathered, rinsed, blanched, blended, filtered, and weighed to attain the amount of three grams (3g) needed for the formulation.50ml of water was added in the blending of the guava leaves.In making the base cream, xanthan gum an emulsifier, was to be dissolved in purified water. Next, ethylenediaminetetraacetic acid (EDTA) a preservative, was also to be added to the mixture. And triethanolamine (TEA), which is used as a neutralizing and emulsifying agent in cosmetic and pharmaceuticals industry for manufacturing of skin care products like creams, ointments and shampoos was also to be added. Then, cetylalcohol- a moisturizer, stearic acid- a lubricant, glycerol monostearate (GMS), mineral oil another moisturizer, and isopropyl myristate (IPM) which often used in pharmaceutical ointments and healing creams because of its ability to soothe and soften skin will also be added in a separate container and heated to 80C with mixing. Next, the first mixture will be added to the second mixture. Then, the mixed raw materials were to be then, added to the base cream until it combines evenly. The cream that is going to be produced is to be placed into a clean container until it cools down.Potential Risk and SafetyThe researcher or respondents might have minor skin allergies if chemicals used in the study are not handled properly.ReferencesFrauenheilk, G. (2011). Topical egyptian bee honey and royal jelly following post surgicalremoval of recurrent cesarean scar endometriosis. Thieme E-Journals, 71Mars, B. (2006). Beauty by Nature. Summertown, TN: Healthy Living PublicationsTenney, D. (1997). Aloe Vera. Pleasant Grove, UT: Woodland Publishing.Aloe Vera.(n.d.).Retrieved on Feb 19, 2013 from on Aug13, 2013 from

V. IntroductionBackground of the studyScars have always been a nuisance in our life. Not only does it leave an unpleasant mark on the body, it also affects the individual psychologically. A scar is any mark left on the skin (Collins English Dictionary, 1819) or other tissue following the healing of a wound. When the skin is wounded and there is a break in the bodys tissues, the body produces more of a protein called collagen as part of the healing process. Collagen builds up where the tissue has been damaged, helping to heal andstrengthen the wound. Since the condition of the skin is a window to ones lifestyle, (Ferrel, Archbold &Cherne, 2004) proper, cheap, and effective cosmetics are needed to restore an individuals flawless skin.Commercial products contain several properties that can help soften the scar and remove unneeded skin. These products are needed to make the skin look flawless again though these commercial products are expensive and a normal citizen cannot afford to waste money on such cosmetics.To address this problem, the researcher made a study focused on formulating an effective and reasonably priced cream made from aloe (Aloe barbadensis), honey (honey), and guava (Psidium guajava) leaf extract. These active ingredients are all known to remove scars and are very much available. Statement of the ProblemThis study was conducted to test the capability of aloe (Aloe barbadensis), honey (Honey), and guava (Psidium guajava) leaf extract in removing scars. It sought to answer the following specific questions:1) What are the properties of aloe (Aloe barbadensis) gel, honey (Honey), and guava (Psidium guajava) leaf extract that help lighten scars?2) Is there any significant difference between the experimental product and the commercial product in terms of:2.1) Odor2.2) Sensation after applying2.3) Scar appearance after 2 weeksSignificance of the StudyThe study would be beneficial to the following:Future ResearchersPeople these days are relying too much on modern medicines and are forgetting about the wonderful properties of aloe (Aloe barbadensis), honey (Honey), guavas (Psidium guajava), etc.People with scarsThe product of this study would be of great help to people with scars.CommunitySurely, people in communities have scars and this study will help those people.Scope and Limitation of the StudyThis study focused on the formulation of a scar-removing cream made using honey (Honey), aloe (Aloe vera), and guava (Psidium guajava) leaf extract. This study was conducted at Bansud National High School - Regional Science High School for Region IV-B MIMAROPA, Pag-asa, Bansud, Oriental Mindoro from July 2013 to January 2014.Related LiteratureScarsA scar is a mark that is left on the skin after a wound or an injury to the surface of the skin has healed. They can be removed using plastic surgery, pressure dressings, silicone gel sheets, etc. Scars naturally heal over time because our body produces a protein called collagen. Some of it breaks down at the site of the wound then the blood supply reduces and the scar gradually becomes smoother, softer, and paler. The healing of scars can be improved by using ointments and other related products. Aloe gelAloe (Aloe barbadensis) gel has been known throughout the world for its amazing benefits to the skin. When broken, it secretes a clear gel that can be applied topically to heal wounds, lighten scars and to soothe skin ( Various studies have been conducted to examine the benefits of the aloe (Aloe barbadensis) vera plant and it was found out that aloe (Aloe barbadensis) vera does in fact have several properties that are effective in treating a variety of skin conditions, from flaky or dry skin, cosmetic ailments, hair and scalp problems to many more.HoneyHoney (Honey) is used commonly as food enhancer. Honey is traditionally known as a natural antibacterial. It is used (even in some allopathic medicine) as a skin healer (for example, helping to heal ulcers). Because of this skin healing action, it may be helpful for people with acne. Honey also softens and moisturizes the skin which is a valuable component in making a scar removing cream. The honey will soften the scar to promote faster fading of the scar.GuavaGuava (Psidium guajava) fruit is a source of anti-oxidants that can help the skin away from aging. Guava (Psidium guajava) leaves also was efficacious to overcome the problem of acne and blackheads on your skin. It also has a high concentration of vitamin C that will aid in the removing of pesky scars. Related StudiesAccording to a book entitled Aloe Vera by Tenney (1997), a group of researchers at the University of Texas in Galveston reviewed many medical studies on aloe vera. Their conclusion was that the aloe gel does promote healing of wounds, burns and frostbite. The aloe is able to penetrate the skin layers to relieve pain, reduce inflammation and increase blood flow to the injured area. In fact, most of the research done with aloe has found that it helps accelerate the healing process of both burns and wounds.Aloe vera can also be used to help prevent scarring and to heal minor scars. The properties of aloe vera help to promote tissue healing as well as remove dead skin. It contains substances called uronic acids that are natural detoxicants and may take part in the healing process by stripping toxic materials of their harmful effects. Aloes enzyme action is able to penetrate tissue and promote the healing and normal growth of the tissue.The study clearly states that aloe vera can be used to heal or prevent scars. It also states that aloe vera promote tissue healing and dead skin removal which will prove to be very useful in the researchers study.A book entitled Beauty by Nature by Mars (2006) stated that After the wound has healed somewhat, applying products to promote healing, such as aloe vera, avocado oil, calendula flowers, castor oil, cocoa butter, comfrey, honey, plantain, and shea butter, will help to treat and prevent scars.This study proves that aloe vera and honey has properties that help lighten scars. This improves the possibility that the product of this study can remove scars.A study conducted by Frauenheilk (2011) aimed to show the effect of topical Egyptian bee honey and royal jelly on wound healing and recurrence rate after removal of recurrent caesarean section scar endometriosis, showed 7 complete exter