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7/30/2019 Rockwood(2011)_Materials Fabrication From Bombyx Mori Silk Fibroin_Nature Protocols
http://slidepdf.com/reader/full/rockwood2011materials-fabrication-from-bombyx-mori-silk-fibroinnature-protocols 1/20
©:>66
KfturdFgdromf#Okm+Fccrohbtsrdsdrvd`+
pratamac
656: | UAC+5 KA+6> | :>66 | kfturd pratamacs
Oktra@umtOakJagjyx garo "sociwarg/ soci os f ukoqud gftdrofc# wbomb bfs bos'taromfccy jddk bohbcy rdhfr`d` ar ots strdkhtb fk` custdr+ bysomofks
bfvd usd` soci fs f suturd gftdrofc ar mdkturods# fk` ot bfs rdmdktcy hfokd` fttdktoak fs f joagftdrofc jdmfusd a sdvdrfc `dsorfjcd prap'
drtods+ Ok pfrtomucfr# tbdsd prapdrtods okmcu`d ots joamagpftojocoty#tbd dfsd wotb wbomb ot mfk jd mbdgomfccy ga`ood`6”4# ots scaw rftda dhrf`ftoak ok vova fk` ots fjocoty ta jd pramdssd` okta guctopcd
gftdrofc argfts rag dotbdr fqudaus sacutoak ar fk arhfkom sac'vdkt5+ Jdmfusd a cfrhd'smfcd muctovftoak a sociwargs ar tbd tdx'tocd ok`ustry# tbdrd frd fjuk`fkt fk` rdfsakfjcd mast saurmds ar
tbos kfturfc pacygdr9 bawdvdr# ar gd`omfc fppcomftoaks# f`dquftddxtrfmtoak fk` prdpfrftoak a tbd mard pratdok os rdquord`+ Eragtbd rfw mamaaks# tbd sdromok magpakdkt gust jd rdgavd` rag
tbd mard ojraok ojdrs+ ]dromok os f hraup a sacujcd hcymapratdoksdxprdssd` ok tbd go``cd soci hcfk` a J+ garo8+ Pbdsd pratdoks mavdr
tbd surfmd a ojraok# tbd soci ocfgdkt mard pratdok# ok tbd mamaakocfgdkt+ Akmd tbos f`bdsovd pratdok os rdgavd`# tbd ojraok ojdrsfrd `ossacvd` okta fk fqudaus sacutoak tbft mfk jd urtbdr pra'mdssd` okta `odrdkt gftdrofcs+ ]agd a tbd gftdrofc argfts tbft
bfvd jddk stu`od` frd sbawk ok Eohurd 6+Pbd accawokh pratamacs mfk bdcp wotb tbd`dsohk fk` ogpcdgdktftoak a f vfrodty a soci'jfsd` joagftdrofcs ar f rfkhd a
patdktofc fppcomftoaks+Kftovd J+ garo soci os magpasd` a soci
ojraok pratdok maftd` wotb sdromok pra'
tdoks+ ]dromoks frd f`bdsovd pratdoks tbftfmmaukt ar :4”=>$ a tbd tatfc sociwarg
mamaak jy wdohbt+ Pbd soci ojraok maksostsa f cohbt mbfok " G
w ~:5 i@f/ fk` f bdfvy
mbfok " G w
~=?> i@f/ cokid` jy f `osuco`djak`5+ ]oci ojraok os f jcami mapacygdr
romb ok by`rapbajom κ'sbddt'argokh
jcamis cokid` jy sgfcc by`rapbocom cokidr sdhgdkts ar spfmdrs+Pbd mrystfccokd rdhoaks frd progfrocy magpasd` a hcymokd'R
rdpdfts# wbdrd R os fcfkokd# sdrokd# tbrdakokd ar vfcokd+ Xotboktbdsd `agfoks cod suj`agfoks tbft frd romb ok hcymokd# fcfkokd#sdrokd fk` tyrasokd5+ Pbd rdsuct os f by`rapbajom pratdok tbft sdc'fssdgjcds ta arg strakh fk` rdsocodkt gftdrofcs+ Pbd agokfkmd a
tbd κ'sbddt'argokh rdhogds wotbok tbd ojraok strumturd ogpfrtstbd pratdok'jfsd` gftdrofcs wotb bohb gdmbfkomfc strdkhtb fk`tauhbkdss+ Pbd tauhbkdss a soci ojdrs os hrdftdr tbfk tbd jdst
syktbdtom gftdrofcs# okmcu`okh Idvcfr2+ Ok tdrgs a strdkhtb# soci'warg soci os supdroar ta maggakcy usd` pacygdrom `dhrf`fjcdjoagftdrofcs sumb fs maccfhdk fk` pacy"c'cfmtom fmo`/ "^CF/+ Pbductogftd tdksocd strdkhtb a J+ garo soci ojdrs os 80> G^f+ Ok mak'
trfst# maccfhdk bfs fk uctogftd tdksocd strdkhtb a >+?”8+0 G^f fk`^CF :2”4> G^f?+ Pbdrdard# soci ojraok os fk dxmdccdkt mfk`o`ftd
pacygdr ar joagd`omfc fppcomftoaks+Ok f``otoak ta tbd ogprdssovd gdmbfkomfc prapdrtods# soci ojraok os
fcsa f dhrf`fjcd gftdrofc+ Bohbcy mrystfccozd` soci `dhrf`ds scawcy#jut tbd rftd ok vova `dpdk`s ak tbd ogpcfktftoak sotd# gdmbfkomfc
Gftdrofcs efjromftoak erag Jagjyx garo soci eojraok
@fkodccd K Zamiwaa`# Zumsfk`f M rd`f# Pukf _ómdc# Rofaqok Xfkh# Gombfdc C Cavdtt % @fvo` C Ifpcfk
@dpfrtgdkt a Joagd`omfc Dkhokddrokh# Puts Tkovdrsoty# Gd`ar`# Gfssfmbusdtts# T]F+ Marrdspak`dkmd sbauc` jd f``rdssd` ta @+C+I+ "`fvo`+ifpcfkNtuts+d`u/+
^ujcosbd` akcokd :: ]dptdgjdr :>669 `ao<6>+6>=2!kprat+:>66+=8?
soi ojo# ov` g Jagjyx garo # o o`y ` ` `o` o yg jogo oo+soi ojo b gij gbo o b g` o o go# g jogojooy# bj `h`o g b y ` j bgoy g`oo` o oggjoozhb + f voy q ho v'oh gb` j ` h oi jogo h oo+ O bo # o` gb` x oi gJ+ garo jo by`h# j# h#go# oj# gob ` bo og+ tb go j ` `oy jogo og# `oho o hooh ` ok votra `o g`# h ovy+
4 ” 6 > @ f y s
: ” 4 @ f y s
= @ f y s
6 @ f
y
6 @ f
y
: @ f y s
Eoh 6 | ]mbdgftom a gftdrofc args fjromftd`
rag soci ojraok usokh jatb arhfkom sacvdkt” fk`
fqudaus'jfsd` pramdssokh fpprafmbds+ Avdrfcc#
tbd soci ojraok dxtrfmtoak pramdss tfids 0 ` fk`
tbd togd wotbok tbd frraws ok`omftds tbd togd
rdquord` ta pramdss tbd soci ojraok sacutoak okta
tbd gftdrofc a mbaomd+
7/30/2019 Rockwood(2011)_Materials Fabrication From Bombyx Mori Silk Fibroin_Nature Protocols
http://slidepdf.com/reader/full/rockwood2011materials-fabrication-from-bombyx-mori-silk-fibroinnature-protocols 2/20
©:>66
KfturdFgdromf#Okm+Fccrohbtsrdsdrvd`+
pratamac
kfturd pratamacs | UAC+5 KA+6> | :>66 | 656=
dkvorakgdkt fk` dfturds a tbd pramdssokh usd` ta prdpfrd tbdsoci gftdrofc+ ]oci `dhrf`ftoak os gd`oftd` jy pratdfsds# wotb tbdpdpto`ds hdkdrftd` gdtfjacozd` jy mdccs?+ J+ garo yfrks bfvd jddk
okmujftd` ok pratdfsd ROU up ta 6: wddis# fk` ot bfs jddk sbawktbft wotb okmrdfsokh okmujftoak togd tbd dkzygd mcdfvd` tbd soci pratdok ft guctopcd camftoaks fcakh tbd mbfoks9 avdrfcc dkzygftom
`dhrf`ftoak wfs gd`oftd` jy surfmd drasoak6>+ Ok f``otoak# tbd`dhrf`ftoak rftd a soci mfk jd fctdrd` jy tbd ga`d a pramdssokh tbdojraok# fs wdcc fs past'pramdssokh trdftgdkts# rdcftd` ta tbd maktdkt
a κ'sbddt mrystfcs fk` dhrdd a arhfkozftoak a tbd kakmrystfccokd`agfoks+ Ok hdkdrfc# tbd dhrf`ftoak rftd dmrdfsds wotb fk okmrdfsdok avdrfcc κ'sbddt maktdkt+ Bawdvdr# ot bfs fcsa jddk sbawk tbft
tbd rftd a `dhrf`ftoak a soci joagftdrofcs `ordmtcy fdmtd` tbd
gdtfjacosg a bugfk gdsdkmbygfc stdg
mdccs "bG]Ms/ fk` maksdqudktcy fctdrd` tbdrftd a astdahdkdsos66+ Pbus# cokis jdtwddksoci ojraok pramdssokh# gftdrofc prapdrtods
sumb fs `dhrf`ftoak rftd fk` joacahomfcfmtovoty bfvd jddk dstfjcosbd`# fk` pravo`df saco` jfsos ar utocozokh soci fs f joagd`o'
mfc gftdrofc ar gfky fppcomftoaks+Fs f suturd gftdrofc# soci ojdrs frd atdk
maftd` ok wfx ta prdvdkt rfyokh fk` patdk'tofc oggukd rdspaksds+ Fctbauhb soci wfs
tbauhbt ta mfusd fccdrhods ok sagd pftodkts#sujsdqudkt rdsdfrmb bfs sbawk tbft sdromokwfs tbd mfusd a tbd oggukd rdspaksds?+
Pbdrdard# sdromok gust jd rdgavd` ragtbd ojraok ta fssurd joamagpftojocoty+ Pbdokcfggftary rdspaksd ta soci ocgs wfs
dvfcuftd` ok votra wotb bG]Ms ar jy sdd`'okh rft G]Ms ak tbd ocgs fk` ogpcfktokhtbdg ok vova6:+ Pbd ok votra rdspaksd ta soci
ojraok wfs sogocfr ta tbd rdspaksd ta mac'cfhdk fk` tossud mucturd pcfstom maktracs+Ok vova# soci sbawd` f cawdr okcfggftary
rdspaksd wbdk magpfrd` wotb maccfhdk fk`^CF6:+ Pbd okcfggftary patdktofc bfs fcsajddk fssdssd` ok votra wotb gfmrapbfhds
ok wbomb soci wfs sbawk ta bfvd cdvdcs a tugar kdmrasos fmtar'ζ# f mytaiokd ok`omf'tovd a fk okcfggftary rdspaksd# sogocfrta tbasd a tossud mucturd pcfstom6=+ Ok f``o'
toak# soci ojraok bfs jddk usd` ok sdvdrfcok vova stu`ods ar jrfok60# sat tossud64# suj'mutfkdaus65 fk` jakd68 fppcomftoaks+
Avdrvodw a tbd pramd`urds
Pbd fppcomftoaks a soci ta f rfkhd a joa'
gftdrofcs# mdcc fk` tossud stu`ods bfvd jddkhrawokh ok rdmdkt ydfrs "Pfjcd 6/+ Pbdsdfppcomftoaks okmcu`d aur mftdharods< tossud
dkhokddrokh# `osdfsd ga`dcs# ogpcfktfjcd`dvomds fk` ruh rdcdfsd+ Pbd gdtba`s usd`ta hdkdrftd tbd gftdrofc argfts usd` ok
tbdsd stu`ods frd okmcu`d` wotbok tbos pra'tamac+ Ear dfmb a tbd soci gftdrofc argfts#tbd rfw soci rag tbd mamaaks orst gust jdtrdftd` ta rdgavd tbd sdromok+ Pbdsd stdps
frd okmcu`d` ok tbd ‒]oci ojraok dxtrfmtoak‟ pratamac+ Pbd dk` rdsuctos fk fqudaus sacutoak a purd soci ojraok tbft mfk jd usd` ok gfky a tbd pratamacs+ Fctdrkftovdcy# tbd sacutoak mfk jd cyapbocozd` ar
cakh'tdrg starfhd ar usd` ta pra`umd gftdrofcs ok arhfkom sacvdkt"6#6#6#=#=#='bdxfcuara':'prapfkac# BEO^/+
Akmd tbd dxtrfmtd` soci ojraok os pramdssd` okta akd a tbd sdvdrfc
argfts dsmrojd` bdrd# tbd okfc stdp os atdk ta ok`umd mrystfccokoty+Mrystfccokoty mfk jd ok`umd` vof twa gdtba`s# dotbdr jy oggdrsoakok fk fcmabac sumb fs gdtbfkac ar dtbfkac ar jy wftdr fkkdfc'
okh+ Fcmabac oggdrsoak os sogpcd fk` quomi jut o tbd rdsdfrmbdrwfkts ta fvao` tbd usd a fk fcmabac# wftdr fkkdfcokh mfk jd usd`+Xftdr fkkdfcokh os tbd pramdss ok wbomb tbd soci gftdrofcs frd okmu'
jftd` ok f bugo` dkvorakgdkt ar sdvdrfc baurs+ Ear gard dtfocd`
tfJcd 6 | Joagd`omfc fppcomftoaks a soci smfac`s+
foo to y Go g
Possud dkhokddrokh
Jakd BEO^ spakhds06#0=#00
Fqudaus spakhds0=#04#05
Dcdmtraspuk ojdrs=5
Mfrtocfhd BEO^ spakhds08
Fqudaus spakhds02”4>
Dcdmtraspuk ojdrs46
]at tossud BEO^ spakhds4:
Fqudaus spakhds4:
By`rahdcs64
Markdfc ^fttdrkd` soci ocgs=6#4=
Ufsmucfr tossuds Pujds::
Dcdmtraspuk ojdrs40”45
Mdrvomfc tossud Fqudaus spakhds48
]iok Dcdmtraspuk ojdrs42#4?
@osdfsd ga`dcs
Jrdfst mfkmdr BEO^ spakhds5>
Fqudaus spakhds56#5:
Futasagfc agokfkt pacymystom
io`kdy osdfsd
Fqudaus spakhds5=
Ogpcfkt dvomds
Fktdroar mrumoftd cohfgdkt Eojdrs50#54
Edgur ddmts BEO^ spakhds68
Gfk`ojucfr ddmts Fqudaus spakhds55#58
@ruh dcovdry
@ruh dcovdry ]pbdrds=0#52”8>
Hrawtb fmtar dcovdry ]pbdrds86
]gfcc gacdmucd ]pbdrds==
Katd< Pbd saurmds ar tbd rdfhdkts fk` dquopgdkt `dsmrojd` ok tbdsd pratamacs frd hovdk akcy fs dxfgpcds+ Dquovfcdkt gftdrofcs mfk jdusd` ukcdss atbdrwosd katd`+
7/30/2019 Rockwood(2011)_Materials Fabrication From Bombyx Mori Silk Fibroin_Nature Protocols
http://slidepdf.com/reader/full/rockwood2011materials-fabrication-from-bombyx-mori-silk-fibroinnature-protocols 3/20
©:>66
KfturdFgdromf#Okm+Fccrohbtsrdsdrvd`+
pratamac
6560 | UAC+5 KA+6> | :>66 | kfturd pratamacs
okargftoak# wd rddr tbd rdf`dr ta tbd stu`y jy Bu dt fc+62# wbomb
`dsmrojds f systdgftom fkfcysos a mrystfccokd ok`umtoak tbrauhbwftdr fkkdfcokh+
]oci ojraok dxtrfmtoak
Pbos pratamac os tbd stfrtokh paokt ok ar`dr ta ajtfok fky a tbdgftdrofc typds costd` dfrcodr‐spakhds# ocgs# ojdrs fk` hdcs+ Aurpratamac os `dsohkd` ta pra`umd akd jftmb rag 4 h a soci mamaaks9
bawdvdr# o gard gftdrofc os rdquord`# tbd vacugds mfk jd smfcd`fppraproftdcy "Eoh+ :/+ Akcy mamaaks tbft caai uk`fgfhd` sbauc`jd usd` ok tbd pramdss+ O kdmdssfry# soci mamaaks mfk jd rdpcfmd` wotb
jfvd ojdrs "rfw soci tdxtocd yfrk/ ok ]tdp 4# fk` tbd rdgfok`dr a tbd
pratamac mfk jd usd` wotbaut mbfkhds+Pbd rdsuctokh sacutoak mfk jd mbfrfmtdr'
ozd` jy f vfrodty a tdmbkoquds jut sagd a tbd vfcuds prdsdktd` bdrd wocc vfry dpdk`okhak tbd cdkhtb a tbd jaocokh togd fk` tbd
saurmd a tbd soci+ Ear dxfgpcd# tbd gacdmucfrwdohbt bfs jddk `dtdrgokd` jy hdc dcdmtra'pbardsos fk` hdc pdrgdftoak mbragftahrf'
pby+ Ear sfgpcds tbft bfvd jddk dhuggd`ar => gok# tbd gacdmucfr wdohbt bfs fjraf` `ostrojutoak mdktdrd` kdfr 6>> i@f+Pbos vfcud mfk okmrdfsd ar sbartdr `dhug'
gokh togds ar dmrdfsd wbdk dhuggd` ar
cakhdr togds6=+ Xrfy fk` ma'waridrs6= bfvd
fcsa dstfjcosbd` f sogpcd gdtba` ta quomicy fk` rdpra`umojcy gdfsurd tbd fgaukt a pratdok `dhrf`ftoak vof cuardsmdkmd spdmtras'
mapy+ Jrodcy# `ocutd sfgpcds ">+6$# wt!vac/wdrd dxmotd` ft :2> kg ok f cuardsmdkmdspdmtrapbatagdtdr fk` tbd jfk` ft =>8
kg wfs fssdssd`+ Ear sfgpcds tbft wdrd`dhuggd` ar => gok# tbd dgossoak vfcudwfs >+85+ Pbos vfcud `dmrdfsd` ar sfgpcdstbft wdrd `dhuggd` ar cakhdr togds fk`
vomd vdrsf ar sbartdr jaocokh togds6=+ Okf``otoak# tbok ocgs a tbd soci sacutoak mfkjd prdpfrd` fk` tbdrgfccy tdstd` ok f odr'
dktofc smfkkokh mfcarogdtdr ta dtdrgokd tbdP
h"682 ¾M/ fk` P
g"6?:”:>= ¾M/6?+
Pbd dxtrfmtoak pramdss tfids 0 `+ O
cyapbocozd` soci os rdquord`# tbdk fk f``o'toakfc = ` wocc jd kdd`d`+ Fqudaus sacu'toaks mfk fcsa jd makmdktrftd` wotbok fk
f``otoakfc :0 b+ Fqudaus soci sacutoaksmfk jd stdrocozd` jy f kugjdr a gdtba`s#okmcu`okh stdrocd octrftoak# ν 'orrf`oftoak
fk` futamcfvokh+ Gard roharaus pramd'`urds# sumb fs ν 'orrf`oftoak:>#:6 fk` futa'mcfvokh "ukpujcosbd` rdsucts/# bfvd jddk
sbawk ta `dhrf`d tbd gacdmucfr wdohbta tbd soci ojraok# fk` tbdrdard orrf`of'toak `asokh ar stdfg stdrocozftoak togokhsbauc` jd warid` aut ar dfmb systdg+ Ok
sagd mfsds# ot os sogpcdr ta stdrocozd tbdokfc pra`umt jdard usd+ Pbd prddrrd`gdtba`s a stdrocozftoak ar dfmb gftdrofc
argft frd katd` wotbok dfmb pratamac+^urood` soci mfk tbdk jd usd` ar f vfrodty a fppcomftoaks# fssuggfrozd` ok Eohurds =”66+
]oci tujds "]tdp :4# aptoaks F fk` J/
Pbdrd frd twa gdtba`s ar prdpfrokh soci tujds+ O stdrocozd` tujds
frd rdquord`# okmujftd tbd tujds ok f sacutoak a 8>$ "vac!vac/ dtbf'kac avdrkohbt fk` tbdk roksd tbdg ok stdrocd wftdr jdard usd+
@op gdtba`+ Pbd orst pratamac amusds ak f sogpcd op gdtba` tamrdftd tbok'wfccd` tujds "Eoh+ =9 aptoak F/+ Xotb tbos gdtba`# soci tujds wotb vfroaus okkdr ofgdtdrs mfk jd ajtfokd`+ Pbd tbomikdssa tbd tujd wfccs mfk fcsa jd vfrod` jy okmrdfsokh ar dmrdfsokh tbd
Jagjyx garo socimamaaks
]quddzd aut dxmdss wftdr
fk` fccaw ta ry avdrkohbt
Zdgavd soci sacutoak eragmfssdttd
Mdktroeuhd twomd ]tard ft 0 °M
F`` ?+= G CoJr ak tap ae
soci eojdrs fk` okmujftd ft
5> °M ear 0 bF`` soci!CoJr ta ofcysos
mfssdttd
@ofcyzd fhfokst uctrfpurd
wftdr ear 02 b
Mut mamaaks fk` `ospasdae warg
Jaoc mamaaks ear => gok ok>+>: G Kf:MA=
Zoksd eojdrs ear :> gok =togds
Eoh : | ]mbdgftom a tbd soci ojraok dxtrfmtoak pramd`urd+ Haokh rag tbd rfw gftdrofc "mamaaks/ ta
tbd okfc fqudaus'jfsd` sacutoak tfids 0 `+
@op gfk`rdc okta
makmdktrftd` soci
sacutoak
@op gfk`rdc okta
gdtbfkac ear 4”6> s
Zdpdft fs kdmdssfry
@ry ear
=> s
@ry tujds avdrkohbt fk`
safi ok safp sacutoak
ta rdgavd
Fccaw soci jdf` ta ruk `awk
tbd gfk`rdc fk` tbdk
jfmi ta tbd top
Eoh = | ]mbdgftom ar gfiokh `oppd` soci tujds+
7/30/2019 Rockwood(2011)_Materials Fabrication From Bombyx Mori Silk Fibroin_Nature Protocols
http://slidepdf.com/reader/full/rockwood2011materials-fabrication-from-bombyx-mori-silk-fibroinnature-protocols 4/20
7/30/2019 Rockwood(2011)_Materials Fabrication From Bombyx Mori Silk Fibroin_Nature Protocols
http://slidepdf.com/reader/full/rockwood2011materials-fabrication-from-bombyx-mori-silk-fibroinnature-protocols 5/20
©:>66
KfturdFgdromf#Okm+Fccrohbtsrdsdrvd`+
pratamac
6565 | UAC+5 KA+6> | :>66 | kfturd pratamacs
Mdccs mfk jd f``d` ftdr tbd sakomftoak stdp fk` proar ta tbd hdcf'toak ta pravo`d bagahdkdaus fk` ddmtovd dkmfpsucftoak ok tbd
hdc+ Fs fk dxfgpcd# wd bfvd dkmfpsucftd` mdccs okta sakomftd` hdcsjy futamcfvokh 0$ "wt!vac/ soci fk` sakomftokh 4 gc a ot usokh tbdgdtba`s jdcaw+ ]dpfrftdcy# bugfk gdsdkmbygfc stdg mdccs wdrd
bfrvdstd` fk` jrauhbt up ta f makmdktrftoak a 4> Ø 6>5 mdccs pdrgc+ F vacugd a 4> ´c a tbd mdcc suspdksoak wfs f``d` ta tbd sako'mftd` soci fk` tbdk okmujftd` ft =8 ¾M fk` 4$ MA
:ar : b jdard
f``okh mdcc mucturd gd`oug:8+
Dcdmtrom murrdkt+ Pbd gftdrofc pra`umd` ok tbos pratamac os fk
f`bdsovd hdc:2+ Pbos hdc os rdvdrsojcd o tbd pacfroty a tbd vactfhd osswotmbd`+ Pbus# o f pasotovd vactfhd os fppcod` ta tbd dcdmtra`d tbftbf` f kdhftovd vactfhd prdvoauscy# tbd hdc wocc jdhok ta arg ak tbd
kdwcy pasotovd dcdmtra`d fk` tbd hdc tbft bf` argd` dfrcodr woccjdhok ta `osfssdgjcd+ Jdmfusd a tbd rdvdrsojcd kfturd a tbos hdc#ot mfk jd usd` ok fppcomftoaks ok wbomb ot os f`vfktfhdaus ta bfvdtukfjcd f`bdsoak+
]oci ocgs "]tdp :4# aptoaks H fk` B/
Eocgs pravo`d f wfy ta smrddk gftdrofcs ar ok votra ar ok vova tdst'
okh# pfttdrk mdccs# dgjd` gacdmucds a oktdrdst fk` tdst dhrf`ftoakfk` rdcdfsd+ Bdrd wd prdsdkt pratamacs ta prdpfrd jatb pfttdrkd`"]tdp :4# aptoak H/ fk` kakpfttdrkd` "]tdp :4# aptoak B/
soci ocgs rag fqudaus sacutoaks "Eoh+ 5/+ Fs tbd pramdssokh oswftdr jfsd`# tbdsd tdmbkoquds adr tbd cdxojocoty ta okmarparftdjoafmtovd gacdmucds=>+ Pa stdrocozd soci ocgs#
tbdy mfk jd okmujftd` ok 8>$ "vac!vac/dtbfkac avdrkohbt fk` tbdk roksd` ok stdrocd^J] ar wftdr+ Eocg tbomikdss fk` garpbac'
ahy mfk jd fssdssd` jy dotbdr gfkufccy gdfsurokh ot wotb f gomragdtdr ar jy vosu'fccy gdfsurokh ot jy ]DG+
Pbd pfttdrkd` soci ocgs hdkdrftd` rag
tbd cfttdr pratamac frd hdkdrfccy 0> ´gtbomi =6+ Pbd pfttdrk `dptb fk` kugjdr a hraavds mfk jd ga`ood` jy mbfkhokh tbd
hrftokh usd` ta prdpfrd tbd pacy`ogdtbyc'socaxfkd "^@G]/ gac`+ Eocg tbomikdss mfkjd maktraccd` jy mbfkhokh tbd makmdktrf'
toak a tbd soci sacutoak# ocutokh ar tbokkdrocgs ar makmdktrftokh ar tbomidr ocgs+ Okf``otoak# pards mfk jd ok`umd` wotbok tbd
soci ocgs jy goxokh vfroaus fgaukts a
^DA okta tbd fqudaus soci sacutoak proar tamfstokh+ Akmd tbd ocgs bfvd jddk rod` fk`wftdr fkkdfcd`# tbd ^DA mfk jd rdgavd`
jy oggdrsoak ok wftdr avdrkohbt+ Ear gardokargftoak# pcdfsd rddr ta Lok dt fc+=:+
]oci gomraspbdrds "]tdp :4# aptoaks O fk L/
Pbd accawokh pratamacs `dsmrojd tbdprdpfrftoak a soci gomra' fk` kfka'spbdrds tbft mfk jd usd` ta dkmfpsucftd
fk` rdcdfsd hrawtb fmtars# sgfcc gac'dmucds ar tbdrfpdutom magpauk`s+ Pbdorst pramdss dsmrojd` usds fk uksfturftd`
ftty fmo` copo` "6#:'`oacdayc'sk'hcymdra'='pbaspbambacokd# @A^M/ ta dkmfpsu'
cftd fqudaus'jfsd` soci sacutoaks wotb f gacdmucd a oktdrdst
"]tdp :4# aptoak O/==+ Akmd vdsomcds bfvd jddk hdkdrftd`# tbdcopo` os rdgavd` fk` tbd gomraspbdrds mfk jd rdsuspdk`d` wbdkrdquord` "Eoh+ 8/+ Pbd sdmak` pramd`urd `dsmrojds tbd argftoak
a gomraspbdrds tbrauhb tbd usd a pbfsd sdpfrftoak jdtwddk soci fk` fkatbdr pacygdr "pacyvokyc fcmabac# UF9 ]tdp :4# aptoak L/=0 "Eoh+ 2/+ Pbos sdmak` gdtba` os pfrtomucfrcy usduc jdmfusd a ots
sogpcomoty fk` fvao`fkmd a arhfkom sacvdkts ok tbd pramdss+Pbd orst pratamac os fk dxfgpcd ak baw ta okmarparftd gacdmucds
a oktdrdst okta soci gomraspbdrds usokh @A^M fs fk dgucsoodr ==+
Pbd fvdrfhd pfrtomcd sozd a spbdrds prdpfrd` wotb tbos gdtba` os~: ´g# wbomb mfk jd `dtdrgokd` wotb dotbdr aptomfc gomrasmapy ar ]DG==+ Fs ar `ruh caf`okh# `odrdkt `ruhs wocc oktdrfmt `odr'dktcy wotb tbd soci fk` tbdrdard tbd caf`okh domodkmy wocc `odr
fgakh vfroaus gacdmucds+ Xd suhhdst tbft sgfcc jftmbds jd tdstd`ok tbd pramdss jdard smfcokh up+ Zdsuspdk`d` gomraspbdrds mfkfcsa jd goxd` wotb soci sacutoak fk` tbdk jd prdpfrd` okta paraus
smfac`s# by`rahdcs ar ocgs+Pbd sdmak` pratamac# wbomb utocozds ^UF# wocc hdkdrftd soci spbdrds wotb f sozd rfkhd jdtwddk =>> kg fk` :> ´g=0+ Pbd sozd
a tbd spbdrds mfk jd maktraccd` jy mbfkhokh tbd makmdktrftoak a tbd soci ar ^UF sacutoaks ar jy sakomftokh ta tbd sacutoak proar tamfstokh tbd ocg+ Pa mbfrfmtdrozd tbdsd spbdrds# f dw tdmbkoquds
bfvd jddk usd`# okmcu`okh ykfgom cohbt smfttdrokh ta dtdrgokd tbdsozd a tbd kfkagdtdr spbdrds# cfsdr cohbt `orfmtoak ta `dtdrgokdtbd sozd a tbd gomraspbdrds fk` ]DG ta ogfhd tbd garpbacahy+
F`` soci tasujstrftd
K a k p f t t d r k d `
^ f t t d r k d `
@ryavdrkohbt
Xftdr fkkdfc
ear 6 `fy
Zdgavd eocgerag sujstrftd
Eoh 5 | ]mbdgftom ar gfiokh pfttdrkd` fk` kakpfttdrkd` soci ocgs+
@ossacvd copo` okmbcaraearg
@ r y u k ` d r k o t r a h d k
E r d d z d ! t b f w m
y m c d s t b d k c y a p b o c o z d
F`` soci!`ruhsacutoak ta copo`
eocg
Copo` dgucsoeodstbd soci
Zdsuspdk` okgdtbfkac
Zdsuspdk`wbdk kdd`d`
Mdktroeuhd fk`rdgavd copo`suspdk`d` ok
gdtbfkac pbfsd
Xftdr os rdgavd` jutsoci!`ruh gomraspbdrds
rdgfok maftd` okf copo` cfydr
Eoh 8 | ]mbdgftom a soci gomraspbdrd prdpfrftoak usokh @A^M+
7/30/2019 Rockwood(2011)_Materials Fabrication From Bombyx Mori Silk Fibroin_Nature Protocols
http://slidepdf.com/reader/full/rockwood2011materials-fabrication-from-bombyx-mori-silk-fibroinnature-protocols 6/20
©:>66
KfturdFgdromf#Okm+Fccrohbtsrdsdrvd`+
pratamac
kfturd pratamacs | UAC+5 KA+6> | :>66 | 6568
Makamfc gomrasmapy wfs fcsa usd` ta `dtdrgokd ga`dc `ruh
caf`okh=0+ @ruhs gfy jd dkmfpsucftd` ok tbdsd spbdrds jy f``'okh tbd `ruh ta tbd soci sacutoak proar ta goxokh wotb tbd ^UF+ Okhdkdrfc# wd stfrt wotb caf`okh f gfss rftoa a 6>><6 soci ta `ruh+
@ruh'caf`okh domodkmy os spdmoom ta dfmb mfsd `dpdk`okh akgacdmucfr wdohbt# mbfrhd fk` by`rapbajomoty# fk` tbdrdard ot gustjd dtdrgokd` ar dfmb ruh+
Dcdmtraspuk soci ojdrs "]tdp :4# aptoak I/
Dcdmtraspokkokh os f pramdss jy wbomb sgfcc'`ofgdtdr ojdrs wotb
cfrhd surfmd frdfs mfk jd pra`umd` "Eoh+ ?9 ]tdp :4# aptoak I/+ Pbddquopgdkt rdquord` os rdcftovdcy sogpcd# jut tbd ojdrs pra`umd`frd gumb sgfccdr ok ofgdtdr magpfrd` wotb makvdktoakfccy spuk
ojdrs=4# fk` tbd pramdss adrs tbd fjocoty ta okmarparftd gacdmucdsa oktdrdst=5+ Ok f``otoak# ojdrs mfk jd fcohkd` dotbdr jy usokh fratftokh gfk`rdc=8 ar jy ok`umokh tbd Bfcc Ddmt=2+ Dcdmtraspukgfts mfk jd stdrocozd` jy oggdrsokh tbd gfts ok 8>$ "vac!vac/
dtbfkac avdrkohbt fk` tbdk sujsdqudktcy
roksokh tbdg ok dotbdr stdrocd wftdr ar J]+Pbos pratamac pra`umds f kakwavdk osa'
trapom gft a soci ojdrs+ Pbd ojdrs wocc bfvd
`ofgdtdrs a cdss tbfk 2>> kg fk` sbauc`fppdfr forcy ukoarg+ ]DG mfk jd usd` tadxfgokd tbd ojdr garpbacahy# `ofgdtdr
fk` arodktftoak+
]oci spakhds "]tdp :4# aptoaks C fk` G/
]oci spakhds pravo`d f vdrsftocd =@ paraus
smfac`okh gftdrofc ar sdvdrfc fppcomf'toaks# fs sbawk ok Pfjcd 6+ Bdrd wd pravo`dpratamacs ta arg fqudaus'jfsd`=? "Eoh+ 6>/
ar BEO^'jfsd` smfac`s0> "Eoh+ 66/+ Pbdfqudaus'jfsd` spakhds bfvd dxmdccdkt
oktdrmakkdmtovoty jdtwddk tbd pards fk` bfvd tbd jdkdot a kat
rdquorokh fk arhfkom sacvdkt+ Pbos gfy jd a oktdrdst o f joafmtovdgacdmucd os okmarparftd` okta tbd soci gftrox `urokh pramdssokh+Pbd BEO^'jfsd` spakhds bfvd sgaatbdr surfmds fcakh tbd pards
fk` bfvd hrdftdr gdmbfkomfc strdkhtb+ Pbrauhb tbos pramdssokhgdtba`# rdokarmokh fhdkts mfk jd f``d` ta tbd soci gftrox ta ur'tbdr okmrdfsd ots gdmbfkomfc prapdrtods06+ Ok f``otoak# ot bfs jddk
sbawk tbft fqudaus'jfsd` spakhds dhrf`d fstdr tbfk BEO^'jfsd`spakhds66# wbomb dkfjcds tbd usdr ta tfocar tbdsd smfac`s ta praldmtspdmoomftoaks+ Jatb a tbdsd spakhd typds gfy jd stdrocozd` jy dotbdr
oggdrsokh tbdg ok 8>$ "vac!vac/ dtbfkac avdrkohbt ar jy futamcfv'okh+ Pbd orst pratamac wocc pra`umd fqudaus'jfsd` soci spakhdstbft frd 64 gg ok `ofgdtdr fk` 6> gg ok bdohbt# fk` tbd sdmak`pratamac wocc pra`umd ft cdfst kokd smfac`s tbft frd ~6: gg ok
jatb bdohbt fk` `ofgdtdr+ ]DG mfk jd usd` ta mbfrfmtdrozd tbdgarpbacahy fk` pard strumturd a tbdsd gftdrofcs+ arasoty mfk jd`dtdrgokd` vof coquo` ospcfmdgdkt0>+
F`` fqudaus socisacutoak ta UF
]oci fk` UF pbfsdsdpfrftd
"sakomftoak aptoakfc/
@ry soci!^UF eocg @ossacvdsoci!^UF eocg
Mdktroeuhd fk`rdgavd UF
Zdsuspdk`wbdk kdmdssfry
Eoh 2 | ]mbdgftom a soci gomraspbdrd prdpfrftoak usokh ^UF+
GftdrOfcsZDFHDKP]
Dxtrfmtoak]oci mamaaks "Pflogf ]balo ar dquovfcdkt/]a`oug mfrjakftd "]ohgf'Fc`romb# mft+ ka+ 046560#bttp<!!www+sohgffc`romb+mag!/Tctrfpurd wftdrCotboug jrago`d "CoJr# ]ohgf'Fc`romb# mft+ ka+ :6=::4#bttp<!!www+sohgffc`romb+mag!/
Makmdktrftokh soci ejraok
Eojraok sacutoak "8”2$# wt!vac/9 ^ZAMD@TZD ]tdp ::/
^DH "6>#>>> GX# ]ohgf'Fc`romb# mft+ ka+ ^5558#bttp<!!www+sohgffc`romb+mag!/Tctrfpurd wftdr
]oci tujds fk` hdc'spuk tujds
Makmdktrftd` soci jraok "^ZAMD@TZD ]tdp :0J/Gdtbfkac "Fmras Arhfkoms# mft+ ka+ 68520>>6>#bttp<!!www+sbdrsmo+mag!/@osb safp
Uartdxd` hdcs fk` sakomftd` hdcs
Fqudaus soci sacutoak# 2$ "wt!vac/Tctrfpurd wftdr
Dcdmtrahdcftd` hdcsFqudaus soci sacutoak# 2$ "wt!vac/pB hdcsFqudaus soci sacutoak# 2$ "wt!vac/
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By`rambcarom fmo` ">+= G BMc# ]ohgf'Fc`romb# mft+ ka+ B6842#bttp<!!www+sohgffc`romb+mag!/
]oci ecgs
Fqudaus soci sacutoak# 2$ "wt!vac/
^fttdrkd` soci ecgs
Fqudaus soci sacutoak# 2$ "wt!vac/^acy`ogdtbycsocaxfkd "^@G]9 ]ychfr` 620# Dccswartb F`bdsovds#mft+ ka+ 620# ]OC DCF]P IOP >+4IH# bttp<!!www+dccswartb+mag!/
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Gox soci
wotb ^DA
@rfw sacutoak
okta f syrokhd
Fppcy f pasotovd vactfhd ta
tbd syrokhd ta okotoftd f ldt
]afi eojdrs ok gdtbfkac eorst fk`
tbdk wftdr ta rdgavd tbd DA
Pbd rdsuctokh eojdrs wocc jd
uk`dr 2>> kg ok `ofgdtdr
)
Eoh ? | Gdtba` ta prdpfrd dcdmtraspuk soci ojdrs+
7/30/2019 Rockwood(2011)_Materials Fabrication From Bombyx Mori Silk Fibroin_Nature Protocols
http://slidepdf.com/reader/full/rockwood2011materials-fabrication-from-bombyx-mori-silk-fibroinnature-protocols 7/20
©:>66
KfturdFgdromf#Okm+Fccrohbtsrdsdrvd`+
pratamac
6562 | UAC+5 KA+6> | :>66 | kfturd pratamacs
]oci gomraspbdrds usokh @A^M
Fqudaus soci sacutoak# 2$ "wt!vac/6#:'@oacdayc'sk'hcymdra'='pbaspbambacokd "@A^M9 Fvfkto ^acfr Copo`s#mft+ ka+ 24>=84^# bttp<!!www+fvfktocopo`s+mag!/Mbcaraarg "Eosbdr ]modktom# mft+ ka+ M:?2'6# bttp<!!www+sbdrsmo+mag!/Gdtbfkac "]ohgf'Fc`romb# mft+ ka+ Z02:2>>>'0M#bttp<!!www+sohgffc`romb+mag!/
Kotrahdk hfs@ruh# sgfcc gacdmucd ar pratdok a oktdrdstTctrfpurd wftdr
Gomraspbdrds "^UF/
Fqudaus soci sacutoak# 2$ "wt!vac/^UF "GX =>”8> i@f9 ]ohgf# mft+ ka+ ^26=5# bttp<!!www+sohgffc`romb+mag!/Tctrfpurd wftdr
Dcdmtraspokkokh
Fqudaus soci sacutoak# 2$ "wt!vac/^acydtbycdkd axo`d "^DA# ?>> i@f9 ]ohgf'Fc`romb# mft+ ka+ 62?045#bttp<!!www+sohgffc`romb+mag!/Tctrfpurd wftdrGdtbfkac "Fmras Arhfkoms# mft+ ka+ 68520>>6># bttp<!!www+sbdrsmo+mag!/
]oci spakhds
Fqudaus soci sacutoak# 5”2$ "wt!vac/
]fct "Eosbdr ]modktom# mft+ ka+ J^=42'6#bttp<!!www+sbdrsmo+mag!/Tctrfpurd wftdr
BEO^'jfsd` spakhds
Cyapbocozd` soci jraok6#6#6#=#=#='bdxfeuara':'prapfkac "BEO^9]ohgf'Fc`romb# mft+ ka+ 6>4::2'6>>H#bttp<!!www+sohgffc`romb+mag!/KfMc "Eosbdr ]modktom# mft+ ka+ J^=42'6#bttp<!!www+sbdrsmo+mag!/Tctrfpurd wftdrGdtbfkac "Fmras Arhfkoms# mft+ ka+ 68520>>6>#bttp<!!www+sbdrsmo+mag!/
DVTO^GDKP
Dxtrfmtoak
Potfkoug smossars "]tfpcds# mft+ ka+ 028?>4#
bttp<!!www+stfpcds+mag!/]gfcc stor jfr "Eosbdr ]modktom#mft+ ka+ 60'46='5:# bttp<!!www+sbdrsmo+mag!/Cfrhd stor jfr "Eosbdr ]modktom#mft+ ka+ 60'46='45# bttp<!!www+sbdrsmo+mag!/]pftucf "Eosbdr ]modktom# mft+ ka+ 60'=8=':4F#bttp<!!www+sbdrsmo+mag!/Hcfss jdfidr "4> gc9 Iogjcd Mbfsd Iogjcd#mft+ ka+ 60>>> 4># bttp<!!www+sbdrsmo+mag!/Hcfss jdfidr "6 cotdr9 Iogjcd Mbfsd Iogjcd#mft+ ka+ 60>>> 6>>># bttp<!!www+sbdrsmo+mag!/Hcfss jdfidr ": cotdrs9 Iogjcd Mbfsd Iogjcd#mft+ ka+ 60>>> :>>># bttp<!!www+sbdrsmo+mag!/^cfstom jdfidr ": cotdrs9 Eosbdr ]modktom#mft+ ka+ >:'4?6'==# bttp<!!www+sbdrsmo+mag!/
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Fcugokug aocBat pcftd "Eosbdr ]modktom# mft+ ka+ 66'6>>'6>>B# bttp<!!www+sbdrsmo+mag!/Cfrhd wdohb jaft "Eosbdr ]modktom#mft+ ka+ >2'8=:'664# bttp<!!www+sbdrsmo+mag!/Gd`oug wdohb jaft "Eosbdr ]modktom# mft+ ka+>2'8=:'66=# bttp<!!www+sbdrsmo+mag!/]gfcc wdohb jaft "Eosbdr ]modktom#mft+ ka+ >2'8=:'66:# bttp<!!www+sbdrsmo+mag!/
Fkfcytomfc jfcfkmd "F%@ Xdohbokh# ga`dcAroak BZ':>># bttp<!!www+fgdromfkwdohb+mag!/Bat bfk` pratdmtars "Jdc'Frt ^ra`umts#mft+ ka+ =2>>>>>>># bttp<!!www+sbdrsmo+mag!/Hrf`uftd` mycok`dr "4> gc9 Eosbdr ]modktom#mft+ ka+ >2'448M# bttp<!!www+sbdrsmo+mag!/]co`d'F'Cyzdr `ofcysos mfssdttd =4>> GXMA#=”6: gc mfpfmoty "Pbdrga ]modktom#mft+ ka+ 5566># bttp<!!www+sbdrsmo+mag!/
@ofcysos mfssdttd juay "Pbdrga ]modktom# mft+ ka+ 550=:# bttp<!!www+sbdrsmo+mag!/]yrokhd ":> gc9 J@ Gd`omfc# mft+ ka+ =>?556# bttp<!!www+sbdrsmo+mag!/Kdd`cds "62H9 J@ Gd`omfc# mft+ ka+ =>46?4# bttp<!!www+sbdrsmo+mag!/Makomfc tujds "4> gc9 J@ Gd`omfc# mft+ ka+ 60'0=:'::# bttp<!!www+sbdrsmo+mag!/Mdktrouhd "Dppdk`ar 42>0Z# mft+ ka+ >::5:=4>6# bttp<!!www+sbdrsmo+mag!/Eoxd`'fkhcd ratar "Dppdk`ar# mft+ ka+ >::5=8:>8# bttp<!!www+sbdrsmo+mag!/
Cyapbocozdr "aptoakfc< usd akcy o cyapbocozd` soci os rdquord`# Cfjmakma#mft+ ka+ 8846>=># bttp<!!www+sbdrsmo+mag!/Erddzdr " √ 2> ¾M9 aptoakfc< usd akcy o cyapbocozd` soci os rdquord`# Pbdrga]modktom# mft+ ka+ TCP:425'?]O'F# bttp<!!www+sbdrsmo+mag!/Iogwopds "Iogjdrcy'Mcfri# mft+ ka+ =0:45# bttp<!!www+sbdrsmo+mag!/
Makmdktrftokh soci ejraok
]yrokhd "6> gc9 J@ Gd`omfc# mft+ ka+ =>?5>0# bttp<!!www+sbdrsmo+mag!/]yrokhd "= gc9 J@ Gd`omfc# mft+ ka+ =>?424# bttp<!!www+sbdrsmo+mag!/Kdd`cds "62H9 J@ Gd`omfc# mft+ ka+ =>46?4# bttp<!!www+sbdrsmo+mag!/]co`d'F'Cyzdr `ofcysos mfssdttd ">+4”=+> gc9 Pbdrga ]modktom# mft+ ka+ 55==>#bttp<!!www+sbdrsmo+mag!/Jdfidr "6>> gc9 Iogjcd Mbfsd Iogjcd# mft+ ka+ 60>>> 6>>#bttp<!!www+sbdrsmo+mag!/Jdfidr "6 cotdr9 Iogjcd Mbfsd Iogjcd# mft+ ka+ 60>>> 6>>>#bttp<!!www+sbdrsmo+mag!/
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F`` fqudaus
soci sacutoak ta
tbd hcfss vofc
^aur sfct avdr
tbd soci+ Zatftd ta
dvdkcy ostrojutd sfct
Fccaw
soci ta hdc
Zdgavd sfct ]tard ar mut
smfeeac` ear usd
Eoh 6> | ]mbdgftom ar gfiokh fqudaus'jfsd` soci spakhds+
@ossacvd soci okBEO^
F`` soci!BEO^sacutoak avdr sfct
Akmd soci pdkdtrftds tatbd jattag ae tbd vofc#
fccaw BEO^ ta dvfparftd
F`` gdtbfkac taok`umd κ'sbddts
Zdgavd gdtbfkac
Zdgavd sfct ]mfeeac` os rdf`y
Eoh 66 | Gdtba` ar prdpfrokh BEO^'jfsd` soci spakhds+
7/30/2019 Rockwood(2011)_Materials Fabrication From Bombyx Mori Silk Fibroin_Nature Protocols
http://slidepdf.com/reader/full/rockwood2011materials-fabrication-from-bombyx-mori-silk-fibroinnature-protocols 8/20
©:>66
KfturdFgdromf#Okm+Fccrohbtsrdsdrvd`+
pratamac
kfturd pratamacs | UAC+5 KA+6> | :>66 | 656?
Cfrhd stor jfr "Eosbdr ]modktom# mft+ ka+ 60'46='45# bttp<!!www+sbdrsmo+mag!/]gfcc `ofcysos mfssdttd juay "Pbdrga ]modktom# mft+ ka+ 550=>#bttp<!!www+sbdrsmo+mag!/Fcugokug aocGomramdktrouhd tujds ": gc mfpfmoty# Eosbdr ]modktom# mft+ ka+ >4'0>2'6=2#bttp<!!www+sbdrsmo+mag!/
]oci tujds
]tfokcdss stddc word mut ta ='okmb cdkhtbs9 hfuhd wocc dpdk` ak rdquord`okkdr `ofgdtdr "d+h+# >+4 gg# GmGfstdr'Mfrr# mft+ ka+ 2?>2I:6#
bttp<!!www+gmgfstdr+mag!/Edftbdr smfcpdc "DG]# mft+ ka+ 8:>0:':># bttp<!!www+sbdrsmo+mag!/Makomfc tujd "64 gc9 J@ Gd`omfc# mft+ ka+ =4:>?5# bttp<!!www+sbdrsmo+mag!/Pwddzdrs "Eosbdr ]modktom# mft+ ka+ >2'22># bttp<!!www+sbdrsmo+mag!/]tyraafg
Hdc'spuk tujdsGfk`rdc ratftoak systdg# mustag gf`d ta ratftd fk` trfksvdrsd:=
Cudr'Cai syrokhd "6 gc9 J@ Gd`omfc# mft+ ka+ =>?5:2# bttp<!!www+sbdrsmo+mag!/Jcukt'top kdd`cd "60 hfuhd9 GmGfstdr'Mfrr# mft+ ka+ 84654F58:#bttp<!!www+gmgfstdr+mag!/Jcukt'top kdd`cd "=> hfuhd9 GmGfstdr'Mfrr# mft+ ka+ 84654F=6#bttp<!!www+gmgfstdr+mag!/Jcukt'top kdd`cd ":8 hfuhd9 GmGfstdr'Mfrr# mft+ ka+ 84654F522#bttp<!!www+gmgfstdr+mag!/]tfokcdss stddc word9 hfuhd wocc `dpdk` ak rdquord` okkdr `ofgdtdr"d+h+# >+4 gg# GmGfstdr'Mfrr# mft+ ka+ 2?>2I:6# bttp<!!www+gmgfstdr+mag!/
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7/30/2019 Rockwood(2011)_Materials Fabrication From Bombyx Mori Silk Fibroin_Nature Protocols
http://slidepdf.com/reader/full/rockwood2011materials-fabrication-from-bombyx-mori-silk-fibroinnature-protocols 9/20
©:>66
KfturdFgdromf#Okm+Fccrohbtsrdsdrvd`+
pratamac
65:> | UAC+5 KA+6> | :>66 | kfturd pratamacs
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Hcfss smoktoccftoak vofcs ":> gc9 Eosbdr ]modktom# mft+ ka+ >='==8'64#bttp<!!www+sbdrsmo+mag!/
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Hcfss vofcs "6+2 gc9 Eosbdr ]modktom# mft+ ka+ >='==?':5F#bttp<!!www+sbdrsmo+mag!/^frfcg "^dmbokdy ^cfstom ^fmifhokh# mft+ ka+ ^G??5#bttp<!!www+sbdrsmo+mag!/]yrokhd "4 gc9 For Potd ^ra`umts# mft+ ka+ F4# bttp<!!www+sbdrsmo+mag!/^cfstom jdfidr ": cotdrs9 Eosbdr ]modktom# mft+ ka+ >:'4?6'==#bttp<!!www+sbdrsmo+mag!/Cfrhd stor jfr "Eosbdr ]modktom# mft+ ka+ 60'46='45# bttp<!!www+sbdrsmo+mag!/Bat pcftd "Eosbdr ]modktom# mft+ ka+ 66'6>>'6>>B# bttp<!!www+sbdrsmo+mag!/
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7/30/2019 Rockwood(2011)_Materials Fabrication From Bombyx Mori Silk Fibroin_Nature Protocols
http://slidepdf.com/reader/full/rockwood2011materials-fabrication-from-bombyx-mori-silk-fibroinnature-protocols 10/20
©:>66
KfturdFgdromf#Okm+Fccrohbtsrdsdrvd`+
pratamac
kfturd pratamacs | UAC+5 KA+6> | :>66 | 65:6
& mfutOak F``okh CoJr ta wftdr rdsucts ok fk dxatbdrgom rdfmtoak9 jd gok`uc a tbd bdft hdkdrftd`+ Xbdk prdpfrokh cfrhdvacugds# wd rdmaggdk` mfrryokh tbos aut ak omd+
mrOtOmfc stdp CoJr bfs f caw `dksoty fk` ots vacugd sbauc` jd tfidk okta fmmaukt wbocd prdpfrokh tbd sacutoak+ Xdsuhhdst f``okh akcy 5>$ a tbd mfcmucftd` vacugd a wftdr fk` tbdk jrokhokh tbd sacutoak up ta tbd kfc vacugd+ ]tor wotb f
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toak wbdkdvdr passojcd ta fvao` tbd ok`umtoak a κ'sbddt wotbok tbd soci+ Pbdrdard# akcy usd tbd kdd`cd wbdk okldmtokh oktatbd mfssdttd+ Gardavdr# bfvd fk f``otoakfc kdd`cd fk` oksdrt ot okta fkatbdr tap part a tbd mfssdttd ta fccaw for ta dsmfpd+Zdgavd tbd dxtrf kdd`cd akmd fcc tbd for bfs jddk purhd`+
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6?| Zdgavd soci rag tbd mfssdttds wotb fkatbdr :>'gc syrokhd fk` fk 62'hfuhd kdd`cd+ ^cfmd soci ok f 4>'gc makomfc tujd+ @dpdk`'okh ak tbd vacugd# dotbdr spcot ot jdtwddk twa tujds "o gard tbfk 0> gc/ ar cc akd tujd fk` usd f mauktdrjfcfkmd a wftdr+
:>| Mdktrouhd ta rdgavd ogpurotods+ ^cfmd ok f mdktrouhd fk` spok ft ?#>>> r+p+g+ "~6:#8>> h / ft 0 ¾M ar :> gok+
:6| Mfrduccy rdgavd tujds rag tbd mdktrouhd fk` dotbdr paur ar trfksdr tbd soci sacutoak wotb f :4 gc popdttd okta fkatbdrmdktrouhd tujd+ Jd surd ta cdfvd fky wbotd eammucdkt ar jrawk gfttdr jdbok`+
::| Zdpdft ]tdps :> fk` :6 fhfok+
:=| Pa `dtdrgokd tbd makmdktrftoak a tbd soci ok sacutoak# gdfsurd tbd wdohbt a f sgfcc wdohb jaft+ Pbdrdftdr# f`` >+4 gc a tbd soci sacutoak ta tbd jaft fk` fccaw ot ta `ry ft 5> ¾M+ Akmd tbd soci os `ry# `dtdrgokd tbd wdohbt a tbd soci fk` `ovo`dot jy >+4 gc+ Pbos wocc yodc` tbd wdohbt pdr vacugd pdrmdktfhd+
mrOtOmfc stdp F jftmb a 4 h a soci mamaaks hdkdrfccy yodc`s :4 gc a 8”2$ "wt!vac/ soci sacutoak+ Pbd sacutoak wocc jdtoktd` ydccaw jut sbauc` jd rdcftovdcy mcdfr fk` scohbtcy gard vosmaus tbfk wftdr+ O tbdrd frd ogpurotods sumb fs wbotd eam'mucdkts ar `fri pfrtomucftds# ot os jdst ta rdmdktrouhd ta rdgavd tbdg+
pfusd paOkt Pbd soci sacutoak mfk jd stard` ft 0 ¾M ar ft cdfst f gaktb+ @dpdk`okh ak tbd puroty# stard` soci wocc dvdktufccy hdc jut hdcftoak togds wocc vfry+ Akmd tbd soci bfs hdccd`# ot mfkkat jd usd` ar pratamacs tbft rdquord sacutoak fk`tbdrdard fkatbdr jftmb wocc kdd` ta jd dxtrfmtd`+
cyboozo ` o:0| Pbd jraok sacutoak ":4 gc ft makmdktrftoak 8”2$ "wt!vac// mfk dotbdr jd usd` fs os ar ot mfk jd cyapbocozd` "aptoak F/ar makmdktrftd` "aptoak J/+ Ear starfhd ar cakhdr tbfk 6 gaktb# tbd soci sacutoak sbauc` jd cyapbocozd`+ Ok tbos arg#tbd soci wocc jd stfjcd ar ydfrs ft raag tdgpdrfturd fk` mfk jd rdmakstotutd` ok BEO^+ Pbd makmdktrftd` sacutoak ":>”=>$#wt!vac/ mfk jd usd` `ordmtcy ar prdpfrokh soci tujds+
7/30/2019 Rockwood(2011)_Materials Fabrication From Bombyx Mori Silk Fibroin_Nature Protocols
http://slidepdf.com/reader/full/rockwood2011materials-fabrication-from-bombyx-mori-silk-fibroinnature-protocols 11/20
©:>66
KfturdFgdromf#Okm+Fccrohbtsrdsdrvd`+
pratamac
65:: | UAC+5 KA+6> | :>66 | kfturd pratamacs
"f/ cyboozo "o/ ◁ tOGOkH = `"o/ @ovo`d tbd fqudaus soci sacutoak okta 4>'gc makomfc tujds wotb ka gard tbfk :> gc pdr tujd+"oo/ ^cfmd vdrtomfccy ok f √ 2> ¾M rddzdr ar sdvdrfc baurs uktoc tbd sacutoak os magpcdtdcy razdk+ O f √ 2> ¾M rddzdr os kat
fvfocfjcd# soci mfk fcsa jd razdk ft √ :> ¾M avdrkohbt ar pcfmd` ok coquo` kotrahdk uktoc tbd sacutoak os razdk+"ooo/ Eac` f Iogwopd avdr tbd tap a tbd tujd fk` fttfmb wotb dotbdr f rujjdr jfk` ar tfpd+ Iddp mfps ar cftdr usd+
"ov/ ^cfmd razdk sfgpcds ak f cyapbocozdr ar :”= ` uktoc fcc a tbd wftdr os rdgavd` rag tbd sacutoak+ Pbd tujd wocc kacakhdr ddc mac`+
"v/ Zdgavd rag cyapbocozdr# mfp fk` stard ft raag tdgpdrfturd ":>”:4 ¾M/+ pfusd paOkt Pbd cyapbocozd` gftdrofc mfk jd stard` ft raag tdgpdrfturd ok`dkotdcy+
"J/ moh oi ojo "o/ ◁ tOGOkH :6”:4 b"o/ F`` 6>> h a ^DH ta ?>> gc a uctrfpurd wftdr ta prdpfrd f 6>$ "wt!vac/ ^DH sacutoak+ Gox wotb f cfrhd stor jfr ak f
gfhkdtom stor pcftd+ Pbd sacutoak sbauc` jd mcdfr akmd tbd ^DH os magpcdtdcy `ossacvd`+ Xbocd wfotokh ar tbd ^DH ta`ossacvd# gavd ta ]tdp :0J"oo/+
"oo/ By`rftd `ofcysos mfssdttd ok f 6>>'gc jdfidr ccd` wotb uctrfpurd wftdr ar f dw gokutds+
"ooo/ Zdgavd tbd mfssdttd rag tbd wftdr fk` tfp tbd jattag a tbd mfssdttd ak f pfpdr tawdc ta `ry+ Jd surd ta bac` tbdmfssdttd ft tbd d`hds fk` `a kat taumb tbd gdgjrfkd+
"ov/ @rfw aut 6> gc a 8”2$ "wt!vac/ soci sacutoak wotb tbd 6>'gc syrokhd wotbaut f kdd`cd+"v/ Fttfmb tbd 62'hfuhd kdd`cd ta tbd syrokhd ccd` wotb jraok sacutoak+"vo/ Oksdrt tbd top a tbd kdd`cd okta f tap part a tbd mfssdttd+
mrOtOmfc stdp Jd mfrduc ta oksdrt lust tbd top a tbd kdd`cd ta fvao` pukmturokh tbd gdgjrfkd+"voo/ ]cawcy oksdrt tbd jraok sacutoak okta tbd mfssdttd+
mrOtOmfc stdp Fvao` bohb sbdfr tbft gfy fccaw prdgfturd fcohkgdkt a tbd κ'sbddts ok tbd soci jraok+"vooo/ Fs tbd mfssdttd jdmagds okeftd`# purhd dxmdss for jy oksdrtokh fkatbdr 62'hfuhd kdd`cd okta tbd atbdr tap part ta
fccaw tbd for ta dsmfpd+"ox/ Ftdr for os rdgavd`# wotb`rfw tbd dxtrf kdd`cd rag tbd mfssdttd+"x/ Eokosb ccokh tbd mfssdttd wotb tbd rdgfokokh jraok sacutoak+ Fctbauhb tbd mfssdttd ok`omftds f gfxogug vacugd a
=+> gc# 6> gc mfk jd oksdrtd`+ @urokh tbd makmdktrftoak pramdss tbos vacugd wocc `dmrdfsd+"xo/ Zdgavd tbd kdd`cd!syrokhd rag tbd mfssdttd+"xoo/ Fttfmb tbd `ofcysos juay akta tbd tap a tbd mfssdttd+"xooo/ ^cfmd tbd mfssdttd okta 6>$ "wt!vac/ ^DH sacutoak fk` mavdr ot wotb fcugokug aoc+"xov/ Ok`omftd tbd togd fk` `ftd tbft tbd mfssdttd wfs f``d` ta sacutoak+ Ear `op'gdtba` tujds# tbd soci os hdkdrfccy
makmdktrftd` ar :> b+ Ear tbd hdc'spuk tujds# tbd sacutoak gust jd gard vosmaus fk` tbdrdard os makmdktrftd` ar ~:: b+"xv/ Zdgavd tbd makmdktrftd` soci sacutoak ftdr tbd `dsord` fgaukt a togd usokh f ='gc syrokhd fk` fk 62'hfuhd kdd`cd+"xvo/ Gdfsurd tbd sacutoak makmdktrftoak jy wdohbokh aut f sgfcc wdohb jaft fk` tbdk f``okh >+6 gc a makmdktrftd` soci
sacutoak ta tbd jaft+ Jdmfusd a tbd bohb vosmasoty a makmdktrftd` soci# wd suhhdst usokh dotbdr cfrhd'aromd popdttdtops ar muttokh a tbd top a tbd popdttd+ @ry tbd sacutoak ft 5> ¾M fk` tbdk wdohb tbd `rod` cg+ Pbd wdohbt a tbd soci`ovo`d` jy tbd vacugd usd` ">+6 gc/ wocc yodc` tbd wdohbt pdr vacugd pdrmdkt+ mrOtOmfc stdp @dpdk`okh ak tbd okotofc soci makmdktrftoak# 6> gc a soci jraok wocc yodc` :”0 gc a makmdktrftd` socisacutoak ftdr `ofcyzokh fhfokst 6>$ "wt!vac/ ^DH ar :>”:: b+ Pbd fgaukt a togd usd` ta makmdktrftd tbd sacutoakgfy kdd` ta jd fctdrd` ar dfmb jftmb+ Pbd makmdktrftoak togd os kat cokdfr# sa mfrd gust jd tfidk ta fvao` hdccokh tbdsoci ok tbd mfssdttd o ot os cdt ok tbd ^DH sacutoak ar taa cakh+ Xotb dxpdrodkmd# tbd rdsdfrmbdr wocc jd fjcd ta vosufccy
`dtdrgokd wbdtbdr tbd soci os makmdktrftd` dkauhb+ O tbd soci os maccdmtd` taa dfrcy# ot mfk dotbdr jd rdpcfmd` okta fmfssdttd fk` fccaw tbd `ofcysos ta pramdd` ar gard togd ar ot mfk jd stard` ft 0 ¾M+ Xd k` tbft starokh tbd soci ar f dw`fys wocc fccaw ot ta rdfmb tbd rdquord` makmdktrftoak+ Ot os suhhdstd` tbft tbd soci jd mbdmid` `focy# fs tbd sacutoak mfk
hdc ftdr ot bfs jddk stard` ar taa cakh "fppraxogftdcy 6”: wddis/+ Pbd sacutoak mfk jd mbdmid` jy fttdgptokh ta usdot ok tbd oktdk`d` pratamac+ Ear dxfgpcd# ar `oppd` tujds# f gfk`rdc mfk jd `oppd` okta tbd sacutoak fk` tbdk ot mfk jdvosufccy `dtdrgokd` wbdtbdr guctopcd jdf`s arg wotbaut mafcdsmokh+ O tbos ammurs# tbdk tbd sacutoak os kat rdf`y+ Pbdrdsuctokh soci sacutoak sbauc` jd vdry vosmaus fk` wocc fppdfr scohbtcy mcau`y wbdk magpfrd` wotb tbd stfrtokh sacutoak+
"xvoo/ Zdgavd tbd kdd`cd fk` trfksdr tbd sacutoak ta :'gc gomramdktrouhd tujds+
mrOtOmfc stdp Jd mfrduc kat ta oktra`umd jujjcds ta tbd sacutoak# fs tbdy frd vdry `omuct ta rdgavd cftdr+
pfusd paOkt Cfjdc fk` stard ft 0 ¾M ar f dw `fys+ Makmdktrftd` soci mfk akcy jd stard` ar f dw `fys# passojcyup ta f wddi# jdard ot hdcs+ Akcy makmdktrftd soci wbdk ot os oktdk`d` ta jd usd` rdcftovdcy saak+
:4| Pbd soci jraok mfk kaw jd usd` ta prdpfrd f kugjdr a `odrdkt gftdrofcs "Eoh+ 6/+ Tsokh tbd makmdktrftd` sacutoak"prdpfrd` ok ]tdp :0J/ yau mfk prdpfrd soci tujds jy dotbdr f sogpcd `op gdtba` ta mrdftd tbok'wfccd` tujds "aptoak F# Eoh+ =/ arjy hdc spokkokh# wbdrdok tbd jraok os dxtru`d` akta f ratftokh gfk`rdc "aptoak J# Eoh+ 0/+ Fctdrkftovdcy# soci sacutoak gfy jd usd`
7/30/2019 Rockwood(2011)_Materials Fabrication From Bombyx Mori Silk Fibroin_Nature Protocols
http://slidepdf.com/reader/full/rockwood2011materials-fabrication-from-bombyx-mori-silk-fibroinnature-protocols 12/20
©:>66
KfturdFgdromf#Okm+Fccrohbtsrdsdrvd`+
pratamac
kfturd pratamacs | UAC+5 KA+6> | :>66 | 65:=
ta prdpfrd by`rahdcs vof vartdxokh# sakomftoak# dcdmtromfc murrdkt ar pB mbfkhd "aptoaks M”E# Eoh+ 4/9 kakpfttdrkd` ar pfttdrkd` socicgs "aptoaks H fk` B# Eoh+ 5/9 soci gomraspbdrds usokh tbd @A^M "aptoak O# Eoh+ 8/ ar UF "aptoak L# Eoh+ 2/ gdtba`9 dcdmtraspuksoci jdrs "aptoak I# Eoh+ ?/9 ar soci spakhds tbft frd dotbdr fqudaus'jfsd` "aptoak C# Eoh+ 6>/ ar BEO^'jfsd` "aptoak G# Eoh+ 66/+
"f/ @o` j ◁ tOGOkH : go j ob vohb `y ` 6 b i"o/ "Aptoakfc/ O paraus tujds frd rdquord`# prdpfrd f sacutoak a 8”2$ "wt!vac/ ^DA fk` mfrduccy f`` tbd fppraproftd
fgaukt ta tbd makmdktrftd` soci sacutoak+ Hdktcy stor wotb f stfokcdss stddc word+ mrOtOmfc stdp Ot os ogpartfkt kat ta oktra`umd jujjcds ta dotbdr sacutoak fs jatb frd bohbcy vosmaus fk` jujjcdsfrd `omuct ta rdgavd+ O jujjcds frd prdsdkt# tbdy wocc mrdftd bacds ok tbd rdsuctokh soci tujds+
"oo/ @op stfokcdss stddc word okta makmdktrftd` soci sacutoak+ Zdgavd tbd word rag tbd soci fk` okvdrt tbd word# fccawokh tbdjdf`s a soci ta dvdkcy ruk `awk tbd word+Xbdk tbd jdf`s mafcdsmd ft tbd jattag a tbd word# eop ot fk` fccaw tbd soci ta ruk jfmi `awk tawfr` tbd dk` a tbdword "try ta maft dvdkcy/+
1 trauJcdsBaatOkH"ooo/ Cdt dxmdss soci jdf`s `rop a a tbd word jfmi okta soci sacutoak+
"ov/ ^cfmd tbd words ok f vofc a gdtbfkac ar 4”6> s+ mrOtOmfc stdp Zdgavd tbd soci'maftd` word rag gdtbfkac fk` fccaw ta `ry ar ~=> s+
"v/ Zdpdft `oppokh pramdss fk` gdtbfkac trdftgdkt :”0 togds ar uktoc tbd word os sumodktcy maftd` wotb soci+ Pbd socigfy fppdfr ukdvdk# jut ot wocc sbroki fs ot `rods+
"vo/ @ry tujds jy stomiokh tbd okvdrtd` soci'maftd` word okta styraafg fk` pcfmd ot ok f ugd baa` avdrkohbt+ Xd suhhdst
gfiokh fk dxmdss kugjdr a tujds# fs sagd a tbd tujds gfy mrfmi `urokh tbd `ryokh pramdss+ pfusd paOkt @rod` tujds mfk jd stard` ar sdvdrfc `fys ft raag tdgpdrfturd+
"voo/ Xbdk soci os `ry# pcfmd tbd words maftd` wotb soci okta f 64'gc makomfc tujd ccd` wotb safpy wftdr fk` safi ar 6 b+"vooo/ Ftdr safiokh# rdgavd tbd tujds rag tbd safpy wftdr fk` mut tbd dk` a tbd soci tujd wotb f smfcpdc ar f rfzar jcf`d+
"ox/ Zdgavd tbd tujd rag tbd word usokh twddzdrs+ Pbd tujd sbauc` sco`d a dfsocy+ O ot os `omuct ta rdgavd wotbautsmrukmbokh tbd tujd# tbdk pcfmd tbd tujd jfmi okta safpy wftdr fk` wfot jdard tryokh fhfok+1 trauJcdsBaatOkH
"x/ Eocc f pcfstom ^dtro `osb "6> mg/ wotb uctrfpurd wftdr+ ^cfmd tbd tujds ok tbd wftdr fk` okmujftd ak f sbfiokh wftdrjftb ar 6 b ta rdgavd rdso ufc safp+
"xo/ ]tard soci tujds ok f pcfstom ^dtro `osb ft raag tdgpdrfturd+ Pbdsd tujds mfk jd stard` ar sdvdrfc gaktbs up ta fmaupcd a ydfrs+ Pbdy frd jrottcd# sa wd suhhdst tbft tbdy jd by`rftd` ok wftdr jdard jdokh gfkopucftd`+
"J/ H' j ◁ tOGOkH 4 go j vohb `y
"o/ ^cfmd tbd gfk`rdc ok tbd gfk`rdc ratftoak systdg+"oo/ Fttfmb f 60'hfuhd kdd`cd ak f 6'gc syrokhd fk` scawcy `rfw up tbd makmdktrftd` soci sacutoak+ Jd surd ta fvao`okmcu`okh for jujjcds+
"ooo/ Zdgavd tbd kdd`cd fk` rdpcfmd wotb f =>'hfuhd kdd`cd+"ov/ ]tfrt tbd gfk`rdc ratftoak systdg+"v/ ]quddzd tbd hdc sacutoak aut a tbd kdd`cd fk` akta tbd gfk`rdc+ Pbd jdr sbauc` jd ukoarg wotbaut fky jdf`s ar
`osmaktokuotods+ Pa ajtfok f ukoarg jdr# f hdkdraus fgaukt a prdssurd os rdquord`+ O sacutoak `ads kat magd aut#rdpcfmd tbd kdd`cd wotb f :8'hfuhd kdd`cd+
mrOtOmfc stdp Pbd wok`okh pfttdrk mfk jd fctdrd` tbrauhb tbd usd a tbd gfk`rdc ratftoak systdg+ O tbdratftoak os makstfkt fk` tbd gfk`rdc asmoccftds jfmi fk` artb# f mrass'bftmbd` `dsohk mfk jd hdkdrftd`+ O f jdr
rukkokh ok tbd sfgd `ordmtoak os rdquord`# tbd gfk`rdc ratftoak systdg sbauc` jd stappd` ftdr akd cfydr fk`tbdk tbd ratftoak rdvdrsd`+ Pbd wfcc tbomikdss ajtfokd` wotb tbos gdtba` wocc `dpdk` ak tbd kugjdr a cfydrs fk`tbd spdd` a tbd trfksvdrsd gatar+
1 trauJcdsBaatOkH"vo/ Akmd tbd soci bfs jddk cfo` akta tbd gfk`rdc# fppcy gdtbfkac ak tap a tbd soci wotb f kdd`cd fk` syrokhd ok ar`dr ta
ok`umd κ'sbddt argftoak wotbok tbd soci jraok+"voo/ Fccaw tbd gdtbfkac ta `ry# fk` tbdk pcfmd tbd gfk`rdc okta f sacutoak a safpy wftdr+"vooo/ Xbdk tbd tujd bfs satdkd`# hrfj tbd tujd ukoargcy wotb f Iogwopd fk` hdktcy pucc ot a+ O tbd tujd `ads kat
rdf`ocy gavd# maktokud ta safi ot ok tbd safp sacutoak+
"ox/ Eocc f pcfstom ^dtro `osb "6> mg/ wotb uctrfpurd wftdr+ ^cfmd tbd tujd ok wftdr fk` okmujftd ok f sbfiokh wftdr jftb ar6 b ta rdgavd rdso`ufc safp+
"x/ ]tard tbd soci tujd ok f pcfstom ^dtro `osb ft raag tdgpdrfturd ar up ta 6 ydfr "`dpdk`okh ak tujd trdftgdkt fk`starfhd mak`otoaks/+
"m/ Ux` h ◁ tOGOkH 6 `"o/ F`lust tbd makmdktrftoak a fk 2$ "wt!vac/ soci sacutoak ta :”4$ "wt!vac/+
7/30/2019 Rockwood(2011)_Materials Fabrication From Bombyx Mori Silk Fibroin_Nature Protocols
http://slidepdf.com/reader/full/rockwood2011materials-fabrication-from-bombyx-mori-silk-fibroinnature-protocols 13/20
©:>66
KfturdFgdromf#Okm+Fccrohbtsrdsdrvd`+
pratamac
65:0 | UAC+5 KA+6> | :>66 | kfturd pratamacs
"oo/ Prfksdr 6 gc a soci sacutoak okta f hcfss vofc fk` mcasd tohbtcy+ mrOtOmfc stdp Pbos pratamac os ar 6 gc a caw'makmdktrftoak soci sacutoak+ Cfrhdr vacugds gfy jd usd`# jut tbdvartdx togd fk` spdd` gfy kdd` ta jd fctdrd`+ Ot os kat kdmdssfry ta usd hcfss vofcs+
"ooo/ ]dmurd tbd vofc ok fk uprohbt pasotoak ak tbd vartdxdr wotb `umt tfpd+"ov/ Uartdx tbd sacutoak ok tbd hcfss vofc ar ~8 gok ft =#:>> r+p+g+ "gfxogug spdd` sdttokh/+ Pbos trdftgdkt sbauc`
okmrdfsd tbd sacutoak turjo`oty+ mrOtOmfc stdp ]oci sacutoaks gfy vfry jdtwddk jftmbds fk` fs f sacutoak fhds9 tbdrdard# tbd vartdx togd gfy kdd`ta jd okmrdfsd` ar `dmrdfsd`+1 trauJcdsBaatOkH
"v/ Maccdmt tbd turjo` sacutoak usokh f 6'gc scop'top syrokhd fvao`okh fky passojcd saco`# stomiy pbfsd+"vo/ Okmujftd turjo` sacutoak avdrkohbt ok tbd syrokhd ar ok f wdcc pcftd ta fccaw hdcftoak+ Okmujftoak ft =8 ¾M wocc `dmrdfsd
tbd hdcftoak togd+ Ear cakh'tdrg starfhd# stard ft 0 ¾M+"voo/ Hdcs mfk jd okldmtd` tbrauhb :6'hfuhd "ar cfrhdr `ofgdtdr/ kdd`cds o kdmdssfry+ Gokogfc armd sbauc` jd sumodkt ta
okldmt f 4$ "wt!vac/ hdc tbrauhb f :6'hfuhd kdd`cd+ O okldmtoak rdquords sujstfktofc armd# tbd top a tbd syrokhd mfk
jd mut usokh f jcf`d "jdard makkdmtokh tbd kdd`cd/ ta rdgavd tbd siok cfydr ft tbd top+
"@/ so` h ◁ tOGOkH 6 `"o/ @ocutd 2$ "wt!vac/ fqudaus soci sacutoak ta 0$ "wt!vac/+"oo/ F`` 4 gc a 0$ "wt!vac/ soci sacutoak ta f 64'gc makomfc tujd+"ooo/ ]akomftd tbd sacutoak ft 4>$ fgpcotu`d ":6 X/ ar => s+
& mfutOak Pbd sakomftokh bark gust jd oggdrsd` ok sacutoak wbocd tbd pawdr os fppcod` ok ar`dr ta prdvdkt `fgfhdta tbd okstrugdkt+ Jd mfrduc kat ta taumb tbd bark ak tbd so`ds a tbd tujd `urokh sakomftoak+
& mfutOak Dxtdk`d` dxpasurd ta uctrfsakomftoak gfy mfusd `fgfhd ta akd‟s bdfrokh+ Tsd a pratdmtovd dfrgus osbohbcy rdmaggdk`d +
1 trauJcdsBaatOkH"ov/ O rdquord`# tbd turjo` sacutoak mfk jd popdttd` okta f wdcc pcftd ar f ^dtro `osb oggd`oftdcy ftdr sakomftoak# proar ta hdcftoak+"v/ Okmujftd tbd sakomftd` sacutoaks ft =8 ¾M avdrkohbt# o kdmdssfry# ta fccaw by`rahdcftoak+
"d/ dh "'h/ ◁ tOGOkH 64 go"o/ F`` 6> gc a 8”2$ "wt!vac/ fqudaus soci sacutoak ta f 4>'gc makomfc tujd+"oo/ Oggdrsd f pfor a pcftokug word dcdmtra`ds okta tbd soci sacutoak+ O tbd dcdmtra`ds `a kat rdfmb tbd sacutoak# mut a
tbd tap partoak a tbd tujd+"ooo/ Fppcy ~:4 U
@Mta tbd pcftokug dcdmtra`ds avdr f dw gokutds+ Xotbok sdmak`s a tbd fppcomftoak a tbd vactfhd# f
vosojcd hdc wocc arg ft tbd pasotovd dcdmtra`d# camiokh ok sagd axyhdk jujjcds ft tbd dcdmtra`d surfmd fs tbd hdc dgfkftds autwfr`+1 trauJcdsBaatOkH
"ov/ Purk a tbd pawdr suppcy wbdk f``otoakfc hdc ka cakhdr maccdmts ak tbd pasotovd dcdmtra`d+"v/ Maccdmt tbd hdc ak tbd pasotovd dcdmtra`d+"vo/ "Aptoakfc/ O f stodr hdc os rdquord`# maccdmt tbd d'hdc okta f syrokhd jy rdgavokh tbd pcukhdr fk` pcfmokh tbd soci ok
tbd apdk dk`+ Pbd κ'sbddt maktdkt a tbd soci os okmrdfsd` wbdk f rfpo` sbdfr armd os fppcod` ta tbd hdc+
"E/ B h ◁ tOGOkH 6> go"o/ F`` >+? gc a 2$ "wt!vac/ fqudaus soci sacutoak ta f hcfss vofc+ Pbos pratamac bfs jddk dstfjcosbd` ar f tatfc sacutoak
vacugd a 6 gc# jut tbd vacugds mfk jd smfcd` up o tbd vacugdtrom rftoa a soci ta fmo` os gfoktfokd` ft 6><6+
"oo/ F`` >+6 gc a >+= G BMc ta tbd vofc+ Pbd gacfroty a tbd BMc mfk jd okmrdfsd` ar `dmrdfsd` `dpdk`okh ak tbd kdd`s a tbd usdr+ Xd bfvd stu`od` hdcs argd` wotb >+6# >+= fk` 6+> G BMc fk` bfvd auk` tbft tbd stokdss okmrdfsd` wotbokmrdfsokh fmo` makmdktrftoak+ Ok f``otoak# wd auk` tbft tbd >+= G BMc hdcs wdrd tbd gast f`bdsovd+
"ooo/ Mcasd mfp fk` stor jy okvdrtokh tbd vofc+"H/ soi eg ◁ tOGOkH : `
"o/ F`` 0 gc a 2$ "wt!vac/ fqudaus soci sacutoak okta f 6>> gg ^dtro `osb+ Pbd cgs pra`umd` jy tbos gdtba` frd hdkdr'fccy 4> ´g tbomi fk` mfk jd dfsocy rdgavd` rag tbd pacystyrdkd `osb+ Ear tbomidr cgs# dotbdr okmrdfsd tbd fgaukt a soci sacutoak ar tbd makmdktrftoak a soci+
"oo/ Fccaw ta `ry avdrkohbt wotbaut mavdrokh tbd `osb+ Fky ga`omftoaks ta dotbdr tbd soci makmdktrftoak ar vacugd gfy
okmrdfsd ryokh togds+"ooo/ Eocc tbd jattag a tbd vfmuug `dsommftar wotb wftdr+"ov/ ^cfmd tbd `ry cgs ok tbd `dsommftar fk` fppcy vfmuug ta tbd vfmuug part+"v/ Fccaw tbd cgs ta wftdr fkkdfc ar 6 `+ mrOtOmfc stdp Pbd wftdr'fkkdfcokh stdp os mrotomfc ta prdvdkt tbd cgs rag `ossacvokh ok wftdr+ Xd bfvd auk`tbft wftdr fkkdfcokh ok`umds κ'sbddt sogocfrcy ta f``okh gdtbfkac jut ta f cdssdr dxtdkt+
7/30/2019 Rockwood(2011)_Materials Fabrication From Bombyx Mori Silk Fibroin_Nature Protocols
http://slidepdf.com/reader/full/rockwood2011materials-fabrication-from-bombyx-mori-silk-fibroinnature-protocols 14/20
©:>66
KfturdFgdromf#Okm+Fccrohbtsrdsdrvd`+
pratamac
kfturd pratamacs | UAC+5 KA+6> | :>66 | 65:4
"vo/ Hdktcy rdgavd tbd cg rag tbd `osb+1 trauJcdsBaatOkH
"voo/ ]tard ft raag tdgpdrfturd ar up ta 6 ydfr "`dpdk`okh ak cg trdftgdkt fk` starfhd mak`otoaks/+
"B/ p` oi eg ◁ tOGOkH 5 b hoh g`# :` oh"o/ ^rdpfrd wari frdf jy cfyokh `awk f sbddt a fcugokug aoc fk` maccdmtokh tbd rdquord` gftdrofcs "f pcfstom popdttd# f
jax a Iogwopds# f pfpdr mup# f kdd`cd# f ]ychfr` jfsd fk` murokh fhdkt# f jattcd a 8>$ "vac!vac/ dtbfkac fk``orfmtoak hrftokh/+ mrOtOmfc stdp O yau frd rdusokh f gac`# siop ta ]tdp :4B"xoo/+ mrOtOmfc stdp Dksurd tbft tbd `orfmtoak hrftokh os rdd a `djros+ ]prfy wotb dtbfkac# wopd fk` jcaw'`ry wotbprdssurozd` for rag tbd jdkmb tap+
"oo/ ^cfmd pfpdr mup ak jfcfkmd# wdohb aut fk fppraproftd fgaukt a jfsd fk` tbdk murokh fhdkt rag tbd ]ychfr` iot+Ear 6 gac` "4 mg Ø 4 mg/ usd 0+4 h a jfsd fk` >+4 h a murokh fhdkt "?<6 rftoa/+
"ooo/ Gox wotb f pcfstom popdttd ar 6 gok+"ov/ ^cfmd ^@G] ok f vfmuug avdk ar =>”5> gok ta rdgavd for jujjcds+
"v/ ^cfmd tbd `orfmtoak hrftokh ok f ^dtro `osb+"vo/ ]cawcy paur tbd ^@G] avdr tbd `orfmtoak hrftokh9 try kat ta oktra`umd jujjcds+"voo/ Akmd tbd hrftokh os mavdrd`# rdgavd fky jujjcds wotb f kdd`cd+"vooo/ Hdktcy gavd tbd ^dtro `osb ta f 5> ¾M avdk ar 0 b ar cdfvd ft raag tdgpdrfturd ar avdr :0 b+"ox/ Zdgavd tbd ^@G] rag tbd `orfmtoak hrftokh+ Jd mfrduc kat ta rop tbd ^@G]+
"x/ Mcdfk tbd gac` wotb f Iogwopd fk` dtbfkac+ pfusd paOkt ^@G] gac`s mfk jd stard` ft raag tdgpdrfturd fk` usd` ok`dokotdcy+ @osmaktokud usd wbdk tbdrd os`fgfhd prdsdkt ak tbd mfstokh fmd+
"xo/ "Aptoakfc/ tbd ^@G] gac` mfk jd pukmbd` ta vfryokh `ofgdtdrs `dpdk`okh ak kdd`+ Ear tbos pratamac# pukmb aut
60'gg `osis rag tbd ^@G]+"xoo/ ^cfmd tbd ^@G] gac`s ok f ^dtro `osb+"xooo/ F`` 6>> ´c a 2$ "wt!vac/ soci sacutoak akta dfmb 60'gg `osi+"xov/ Fccaw tbd cgs ta `ry avdrkohbt+"xv/ Eocc tbd jattag a tbd vfmuug `dsommftar wotb wftdr+
mrOtOmfc stdp ^cfmd tbd `ry cgs ok tbd `dsommftar fk` fppcy vfmuug ta tbd vfmuug part+"xvo/ Fccaw tbd cgs ta wftdr fkkdfc ar 6 `+"xvoo/ Fccaw tbd cgs ta `ry ar 6> gok fk` hdktcy pddc cgs rag tbd gac` usokh twddzdrs+
"xvooo/ Pa ok`omftd tbd pfttdrkd` so`d a tbd cg# pcfmd fk d`hd a tbd kakpfttdrkd` so`d akta f ^ast'ot katd+ ]tard ft raagtdgpdrfturd uktoc usd+
"O/ Gob "@apm/ ◁ tOGOkH 0+4 `"o/ F`` :>> gh a @A^M copo` paw`dr ta f hcfss tdst tujd+"oo/ Ok f baa`# f`` 6 gc a mbcaraarg fk` wfot uktoc tbd @A^M os magpcdtdcy `ossacvd`9 tbd sacutoak wocc turk mcdfr+
& mfutOak Mbcaraarg os f bfzfr`aus sujstfkmd ikawk ta jd mfrmokahdkom9 wdfr prapdr sfdty dquopgdkt fk` wariokso`d f mbdgomfc ugd baa`+
"ooo/ Zacc tbd tujd ta dvdkcy maft tbd oktdroar a tbd tujd wotb f tbok# bagahdkaus cfydr+ Xbocd raccokh tbd tujd# `ry autsacutoak wotb kotrahdk hfs eaw+ Xbdk tbd cg os kdfrcy `ry# tbd eaw rftd mfk jd okmrdfsd`+
"ov/ Cdfvd tbd tujd ta `ry ok tbd baa` ar => gok+
pfusd paOkt Hcfss tujds mfk jd maftd` wotb @A^M fk` stard` ft √ :> ¾M uktoc kdd`d`+"v/ Ok f 6+4'gc gomramdktrouhd tujd# f`` :>> ´c a 6> gh gc √ 6 sacutoak a tbd `ruh# pratdok ar mbdgomfc ta jd dkmfpsu'
cftd` ta 6 gc a 2$ "wt!vac/ soci sacutoak+ O `dsord`# fcsa prdpfrd f sacutoak a 6 gc a 2$ "wt!vac/ soci wotb :>> ´c a
uctrfpurd wftdr ar f maktrac sfgpcd+"vo/ By`rftd tbd copo` cg wotb tbd soci fk` `ruh sacutoak "ar maktrac sacutoak# o fppcomfjcd/+ Jy f``okh tbd fqudaus sacu'
toak# tbd wftdr wocc scawcy `ossacvd tbd copo` cg rag tbd hcfss tujd surfmd+ Pbd sacutoak wocc jdmagd gard turjo` fstbd copo` cg os by`rftd` fk` dvdktufccy tbd copo` cg wocc `osfppdfr rag tbd hcfss# cdfvokh ot mcdfr+
"voo/ Akmd tbd cfydr os rdby`rftd`# `ocutd wotb = gc a uctrfpurd wftdr# gfiokh surd ta hdt tbd dktord copo` cfydr a tbd wfcc a tbd hcfss tujd+
"vooo/ Prfksdr tbd sacutoak rag yaur hcfss tujd ta f 64'gc makomfc tujd ar rddzd'tbfwokh+"ox/ ]ujgdrhd tbd tujd ok coquo` kotrahdk ar 64 gok ar uktoc tbd coquo` kotrahdk staps jaocokh+
& mfutOak Coquo` kotrahdk os dxtrdgdcy mac` fk` dxpasurd ta ot gfy mfusd jurks+ Jd mfrduc wbdk bfk`cokh ot fk` wdfrprapdr sfdty dquopgdkt+
"x/ Vuomicy gavd tbd tujd ta f =8 ¾M wftdr jftb ar 64 gok "mbdmi tbft tbd maktdkts frd tatfccy tbfwd` ft tbd dk` a dfmb 64'gok tbfw/+
7/30/2019 Rockwood(2011)_Materials Fabrication From Bombyx Mori Silk Fibroin_Nature Protocols
http://slidepdf.com/reader/full/rockwood2011materials-fabrication-from-bombyx-mori-silk-fibroinnature-protocols 15/20
©:>66
KfturdFgdromf#Okm+Fccrohbtsrdsdrvd`+
pratamac
65:5 | UAC+5 KA+6> | :>66 | kfturd pratamacs
"xo/ Zdpdft ]tdps ?”6> twomd gard ta magpcdtd tbrdd rddzd'tbfw mymcds "ok ar`dr ta mrdftd f gard bagahdkaus sozd`ostrojutoak a spbdrds/+
"xoo/ Eocc f 6>>'gc jdfidr wotb 4> gc a uctrfpurd wftdr+ F` f stor jfr fk` gavd tbd jdfidr ta f stor pcftd+"xooo/ ^opdttd tbd `ocutd` sacutoak okta tbd 6>> gc a uctrfpurd wftdr vdry scawcy "`rop jy `rop/ wotb fst storrokh+"xov/ Prfksdr tbd `ocutd` sacutoak ta f 4>'gc makomfc tujd fk` rddzd ot ft √ 2> ¾M ar ft cdfst 4 b+ @a kat dxmdd` 0> gc a
sacutoak pdr tujd fs tbd sacutoak gfy dxpfk` `urokh rddzokh+"xv/ Cyapbocozd ar :”= `+"xvo/ Ftdr cyapbocozftoak# trfksdr => gh a cyapbocozd` pra`umt okta f cfrhd :'gc gomramdktrouhd tujd fk` f`` : gc a purd
gdtbfkac+& mfutOak Gdtbfkac os f bfzfr`aus sujstfkmd9 wd rdmaggdk` usokh tbd prapdr sfdty dquopgdkt+ Ok f``otoak#gdtbfkac os f efggfjcd sacvdkt9 tbdrdard# saurmds a ohkotoak sbauc` jd rdgavd` jdard usd+
"xvoo/ Okmujftd tbd sfgpcd ar => gok ft raag tdgpdrfturd "fk okmujftoak togd a akcy 6> gok os rdmaggdk`d` o f pratdokar gdtbfkac'sacujcd `ruh os jdokh dkmfpsucftd`/+
"xvooo/Prfksdr tbd sacujcd partoak ta f kdw :'gc gomramdktrouhd tujd+
"xox/ Mdktrouhd tbd tujds ft 6>#>>> r+p+g+ ar 4 gok ft 0 ¾M ok f gomramdktrouhd+"xx/ @osmfr` tbd gdtbfkac supdrkftfkt okta f prapdr wfstd jattcd fk` `ry tbd pdccdt avdrkohbt ok tbd baa`+ ]tard ft 0 ¾M+"xxo/ Pa rdsuspdk` tbd gomraspbdrds# rst wfsb tbd gomraspbdrds jy f``okh : gc a wftdr ta tbd pdccdt fk` tbdk mdktrouhd
ft 6>#>>> r+p+g+ ar 4 gok ft 0 ¾M+"xxoo/ Zdgavd tbd wftdr fk` rdsuspdk` gomraspbdrds ok tbd `dsord` wftdr ar judr+ O tbd gomraspbdrds bfvd fhhrdhftd`#
tbdy mfk jd `ospdrsd` jy sakomftokh tbd sacutoak ar fjaut 6> s ft =>$ fgpcotu`d "~:> X/+"L/ Gob "pUf/ ◁ tOGOkH : `
"o/ ^rdpfrd f 4$ "wt!vac/ ^UF sacutoak jy f``okh >+:4 h a ^UF ta 0 gc a uctrfpurd wftdr+ Bdft ta 5> ¾M ta `ossacvd tbd ^UF+"oo/ Prfksdr tbd sacutoak okta tbd 6>'gc hrf`uftd` mycok dr fk` f`lust tbd vacugd a tbd sacutoak up ta 4 gc wotb
uctrfpurd wftdr+"ooo/ Eoctdr tbd ^UF sacutoak wotb f >+04'´g ctrftoak ukot+
pfusd paOkt ]tard tbd ^UF sacutoak ft raag tdgpdrfturd ar ka gard tbfk = gaktbs+"ov/ @ocutd soci sacutoak ta 4$ "wt!vac/ wotb uctrfpurd wftdr+"v/ F`` 6 gc a 4$ "wt!vac/ soci fk` 0 gc a 4$ "wt!vac/ ^UF okta f 64'gc makomfc tujd fk` hdktcy gox+ Xbocd scawcy
gavokh tbd prajd up fk` `awk# sakomftd tbd sacutoak ar => s ft :4$ fgpcotu`d+& mfutOak Pbd sakomftokh bark gust jd oggdrsd` ok sacutoak wbocd tbd pawdr os fppcod` ok ar`dr ta prdvdkt `fgfhdta tbd okstrugdkt+ Jd mfrduc kat ta taumb tbd bark ak tbd so`ds a tbd tujd `urokh sakomftoak+
& mfutOak Dxtdk`d` dxpasurd ta uctrfsakomftoak gfy mfusd `fgfhd ta akd‟s bdfrokh+ Tsd a pratdmtovd dfrgus osbohbcy rdmaggdk`d +
"vo/ ^aur tbd sacutoak okta f ^dtro `osb fk` fccaw ot ta mavdr tbd jattag a tbd `osb dvdkcy+"voo/ @ry tbd sacutoak avdrkohbt ok f ugd baa`+
pfusd paOkt Ftdr `ryokh# tbd ocg mfk jd stard` ar f dw wddis+ Mavdr fk` sdfc tbd `osb wotb ^frfocg fk` stardot ft raag tdgpdrfturd+
"vooo/ ^ddc a tbd cg rag tbd `osb fk` pcfmd akd cg ok f 4>'gc makomfc tujd+"ox/ F`` :> gc a `doakozd` wftdr ta tbd tujd9 sbfid ar => gok ft raag tdgpdrfturd ta `ossacvd tbd cg+
1 trauJcdsBaatOkH"x/ Mdktrouhd ft 66#>>> r+p+g+ ar :> gok ft 0 ¾M fk` `osmfr` tbd supdrkftfkt+
"xo/ Zdsuspdk` tbd pdccdt ok 4 gc a uctrfpurd wftdr+ ]akomftd ar 64 s ft 64$ fgpcotu`d# o kdmdssfry+Aptoakfc< usd tbd suspdksoak "maktfokokh jatb kfka' fk` gomraspbdrds/ ar cyapbocozd fk` stard tbd `ry gftdrofc+
"I/ d ej ◁ tOGOkH 6 `"o/ ^rdpfrd f sacutoak a 4$ "wt!vac/ ^DA ok wftdr ok f :>'gc hcfss smoktoccftoak vofc+ Tsd f sgfcc stor jfr ta dksurd
prapdr goxokh+ pfusd paOkt Pbd ^DA wocc tfid togd ta `ossacvd okta sacutoak# sa tbos sbauc` jd `akd fbdf` a togd+ Pbos sacutoakmfk jd stard` ft 0 ¾M ar 6 ydfr+
"oo/ F`` 4 gc a tbd 4$ "wt!vac/ ^DA "?>>#>>> h gac √ 6/ sacutoak okta :> gc a 2$ "wt!vac/ soci sacutoak ta hdkdrftd fkfqudaus sacutoak a 5+0$ "wt!vac/ soci!6$ "wt!vac/ ^DA+
mrOtOmfc stdp F bohb vosmasoty# jdmfusd a pacygdr makmdktrftoak fk` gacdmucfr wdohbt# gust jd gdt ok ar`dr artbd pacygdr gacdmucds ta dktfkhcd fk` arg f ldt+ O dotbdr tbd makmdktrftoak ar gacdmucfr wdohbt a tbd pacygdr ostaa caw# akcy jdf`s wocc arg+ Bdkmd# wd bfvd okmcu`d` ^DA ok tbos pratamac ta pragatd jdr argftoak jy okmrdfsokhtbd avdrfcc pacygdr makmdktrftoak+
"ooo/ ]cawcy stor tbd jcdk`d` sacutoak ar ~6> gok ft 0 ¾M ta ajtfok f bagahdkaus sacutoak+ Fvao` bohb sbdfr `urokh goxokhtbft gfy mfusd prdgfturd mrystfccozftoak a tbd soci+
7/30/2019 Rockwood(2011)_Materials Fabrication From Bombyx Mori Silk Fibroin_Nature Protocols
http://slidepdf.com/reader/full/rockwood2011materials-fabrication-from-bombyx-mori-silk-fibroinnature-protocols 16/20
©:>66
KfturdFgdromf#Okm+Fccrohbtsrdsdrvd`+
pratamac
kfturd pratamacs | UAC+5 KA+6> | :>66 | 65:8
"ov/ @rfw up 6> gc a tbd soci!^DA sacutoak okta f 6> gc syrokhd+"v/ Fttfmb f 65'hfuhd kdd`cd ta tbd syrokhd+"vo/ Gaukt tbd syrokhd ak tbd syrokhd pugp+"voo/ ^cfmd f maccdmtoak surfmd ft f `ostfkmd a 8”:> mg rag tbd top a tbd kdd`cd+ Gfky surfmds mfk jd usd` ta maccdmt
dcdmtraspuk jdrs fs cakh fs tbdy mfk jd dcdmtromfccy hrauk`+ F sogpcd surfmd tbft maksosts a f podmd a mfr`jafr`
mavdrd` wotb fcugokug aoc os sumodkt+"vooo/ Fttfmb tbd pasotovd vactfhd cdf` ta tbd kdd`cd ak tbd syrokhd fk` tbd hrauk` cdf` ta tbd maccdmtoak surfmd+"ox/ ]dt tbd murrdkt ta scohbtcy fjavd > F+ Purk ak tbd bohb vactfhd fk` syrokhd pugp+
& mfutOak Dcdmtraspokkokh os pdrargd` ft f bohb vactfhd+ Jd mfrduc wbocd tbd bohb vactfhd os ak fk` `a kat taumbmbfrhd` surfmds sumb fs tbd kdd`cd ar pasotovd vactfhd cdf`+
"x/ F`lust tbd sacutoak eaw rftd ">+>6”>+>= gc gok √ 6/# dcdmtrom patdktofc "2”64 IU/ fk` tbd `ostfkmd jdtwddk tbdmfpoccfry top fk` tbd maccdmtoak smrddk "8”:> mg/ ta ajtfok f stfjcd ldt+
mrOtOmfc stdp Pbd dcdmtrom dc` strdkhtb os rdquord` ta okotoftd tbd ldt fk` ft tbd cawdr cogot jdf` `ddmts frddvo`dkt ak tbd jdrs+ Ft tbd bohbdr cogot# soci jdrs trfksotoak rag f rauk` garpbacahy ta f rojjak'sbfpd` mrass'
sdmtoak+ Ot bfs fcsa jddk katd` tbft wotb fk okmrdfsd ok vactfhd tbd jdr `ofgdtdr okmrdfsds jdmfusd a f``otoakfc gfss trfkspart wotb tbd bohbdr dcdmtromfc armd+ Jy okmrdfsokh tbd `ostfkmd jdtwddk tbd spokkdrdt fk` tbd maccdmtokhsurfmd# tbd jdr `ofgdtdr mfk jd rd`umd`+ Eokfccy# tbd eaw rftd sbauc` jd f`lustd` sa tbft tbd sacutoak os makstfktcyjdokh suppcod` ta tbd top wotbaut fccawokh dxmdss sacutoak ta `rop0:+
"xo/ Maccdmt tbd soci jdrs uktoc tbd `dsord` tbomikdss os fmbodvd`+
"xoo/ Oggdrsd tbd jdr gfts ok f ?>$ "vac!vac/ gdtbfkac!wftdr sacvdkt ar :> gok ta ajtfok wftdr'oksacujcd jdr gfts+& mfutOak Gdtbfkac os f bfzfr`aus fk` efggfjcd sujstfkmd9 usd prapdr pdrsakfc sfdty dquopgdkt fk` rdgavd fcc saurmds a ohkotoak jdard usd+
"xooo/ Okmujftd tbd gdtbfkac'trdftd` jdr gfts ok uctrfpurd wftdr ak f rdmopramftokh sbfidr avdrkohbt ta rdgavd tbd ^DA
rag tbd gfts+"xov/ @ry tbd jdr gfts ok f mbdgomfc ugd baa`+
"c/ fq'j` h ◁ tOGOkH 4 `"o/ ^rdpfrd tbd sfct wotb tbd pfrtomcd sozds a oktdrdst+ ]tfmi tbd sodvds wotb tbd cfrhdst gdsb ak tap fk` tbd sgfccdst
gdsb ak tbd jattag+ F`` sfct fk` sbfid voharauscy+ Zdpdft uktoc tbd `dsord` fgaukt a sfct os maccdmtd`+ mrOtOmfc stdp Ot os ogpartfkt ta katd tbft tbd pard sozds wocc jd scohbtcy sgfccdr tbfk tbd sfct pfrtomcds usd`# fstbd sfct os pfrtofccy `ossacvd` wbocd tbd soci hdcs+ Pbdrdard# ot os ogpartfkt ta stfrt wotb sfct pfrtomcds tbft frd scohbtcycfrhdr tbfk tbd rdquord` pard vacugd+
1 trauJcdsBaatOkH"oo/ Xdohb 0 h a sfct ok wdohbokh jafts# akd ar dfmb gac`+
"ooo/ Xotb f 4'gc syrokhd# fcoquat jraok sacutoak okta pcfstom maktfokdrs# : gc pdr gac`+ Katd< Ear sfct pfrtomcds 84> ´g fk`cfrhdr# usd 2$ "wt!vac/ soci sacutoak+ Ear sgfccdr sfct pfrtomcds# usd 5$ "wt!vac/ soci sacutoak+ Pbd sozd fk` sbfpd a tbdsmfac`s gfy jd vfrod` jy mbfkhokh tbd gac`s+ Pbd rftoa a sfct ta soci ok sacutoak sbauc` jd gfoktfokd` ft :4<6+Pbd kugjdr a smfac`s mfk dfsocy jd smfcd` up jy ccokh f``otoakfc gac`s+
"ov/ ]cawcy paur tbd sfct ak tap a tbd jraok sacutoak ok tbd gac` wbocd ratftokh tbd maktfokdr sa tbft tbd sfct os ukoarg+"v/ Pfp tbd maktfokdr hdktcy ak tbd jdkmb tap ta rdgavd for jujjcds+"vo/ Mcasd tbd mfp fk` fccaw tbd sacutoak ta sdttcd avdrkohbt ft raag tdgpdrfturd+ Pbd soci jraok sbauc` hdc ok 6”: `+"voo/ Akmd tbd soci bfs hdccd`# rdgavd tbd co`s fk` pcfmd tbd gac`s ok f :'cotdr jdfidr wotb uctrfpurd wftdr "tbrdd maktfokdrs
pdr : cotdrs a wftdr/+"vooo/ Prfksdr tbd jdfidr ta f stor pcftd fk` stor+"ox/ Mbfkhd tbd wftdr :”= togds pdr `fy ar : ` "0”5 wfsbds ok tatfc/+
"x/ Zdgavd tbd smfac`s rag tbd gac`s fk` rdpcfmd tbdg ok rdsb wftdr ar fk f``otoakfc `fy+"xo/ ]tard tbd smfac`s ok uctrfpurd wftdr ok 4> gc tujds ft 0 ¾M uktoc kdd`d` ar usd+ Ear cakh'tdrg starfhd# `ry tbd
smfac`s fk` stard tbdg ft raag tdgpdrfturd+"xoo/ Jdard usd# mut by`rftd` smfac`s okta tbd `dsord` `ogdksoaks+ Futamcfvd ta stdrocozd+ Pa mut tbd smfac`s ta sgfccdr
`ogdksoaks# wd suhhdst `ospasfjcd joapsy pukmbds ta ajtfok ukoarg `osis+
"G/ BEOp'j` h ◁ tOGOkH 2 `"o/ ^rdpfrd 68$ "wt!vac/ soci!BEO^ sacutoak+ Ear f vacugd a 6> gc kfc sacutoak# paur ? gc a BEO^ avdr 6+8 h a
cyapbocozd` soci+& mfutOak BEO^ os costd` jy tbd Bfzfr`aus Gftdrofcs Okargftoak ]ystdg fs f bdfctb bfzfr` cdvdc =9 ot mfk mfusd jurksjatb tapomfccy fk` o okbfcd`+ Xdfr prapdr sfdty dquopgdkt fk` wari okso`d f mbdgomfc ugd baa`+1 trauJcdsBaatOkH
"oo/ Xrfp tbd co` wotb ^frfcg fk` iddp ot ft raag tdgpdrfturd avdrkohbt ar uktoc fcc tbd soci os `ossacvd`+
7/30/2019 Rockwood(2011)_Materials Fabrication From Bombyx Mori Silk Fibroin_Nature Protocols
http://slidepdf.com/reader/full/rockwood2011materials-fabrication-from-bombyx-mori-silk-fibroinnature-protocols 17/20
©:>66
KfturdFgdromf#Okm+Fccrohbtsrdsdrvd`+
pratamac
65:2 | UAC+5 KA+6> | :>66 | kfturd pratamacs
"ooo/ Xbocd tbd soci os `ossacvokh# prdpfrd tbd sfct wotb tbd pfrtomcd sozds a oktdrdst+ ]tfmi tbd sodvds wotb tbd cfrhdst gdsbak tap fk` tbd sgfccdst gdsb ft tbd jattag+ F`` sfct fk` sbfid voharauscy+ Zdpdft uktoc tbd `dsord` fgaukt a sfctos maccdmtd`+
"ov/ Xdohb =+0 h a KfMc fk` pcfmd ot ok f hcfss sbdcc vofc+ Zdpdft ar dohbt gard vofcs+
mrOtOmfc stdp O cfrhdr smfac`s frd rdquord`# tbd gac` mfk jd dxmbfkhd` wotb f cfrhdr akd fk` tbd sfct wdohbt mfk
jd smfcd` wotb f :><6 rftoa wotb tbd soci gfss ok sacutoak+ Jd mfrduc ta mbaasd f gac` gftdrofc tbft wocc kat oktdrfmt wotbBEO^+ BEO^ os bohbcy marrasovd# sa gdtfc sbauc` jd fvao`d`+ Ok f``otoak# ar cfrhdr smfac`s tbd gdtbfkac trdftgdkt fk``ofcysos togds gfy kdd` ta jd smfcd` up ta dksurd pdkdtrftoak a tbd sacvdkt gacdmucds okta tbd mdktdr a tbd smfac`s+
"v/ "Aptoakfc/ O yau frd f``okh f rdokarmokh fhdkt sumb fs by`raxyfpftotd# wdohb ot fk` f`` ot ta tbd sfct bdrd+ Gox wdcc#jatb ok tbd wdohb `osb fk` ok tbd gac`+
"vo/ Pfp tbd maktfokdr hdktcy ak tbd jdkmb tap surfmd ta cdvdc tbd sfct ok tbd maktfokdr+"voo/ Ok f ugd baa`# f`` 6 gc a soci sacutoak ta dfmb maktfokdr a sfct vdry quomicy usokh f 4'gc syrokhd+ Oggd`oftdcy mfp
dfmb maktfokdr ta rd`umd sacvdkt dvfparftoak+"vooo/ Xrfp dfmb mfp wotb ^frfcg fk` fccaw soci sacutoak ta pdkdtrftd tbd sfct "6”: `/+
"ox/ Akmd tbd sfct fppdfrs ‒wdt‟ fk` tbd soci sacutoak bfs rdfmbd` tbd jattag a tbd vofc# ukmfp tbd vofcs ta fccaw BEO^ tadvfparftd ar 6 `+& mfutOak Iddp tbd smfac`s ok tbd baa`+ pfusd paOkt Pbd `rod` smfac`s mfk jd stard` ft raag tdgpdrfturd ar sdvdrfc `fys+
"x/ Xbdk tbd soci fppdfrs `ry fk` `dtfmbd` rag tbd maktfokdr wfccs# f`` 6 gc a gdtbfkac ta dfmb maktfokdr fk` rdmfp+
Fccaw tbd gdtbfkac ta sddp tbrauhb tbd smfac` ar 6 `+"xo/ Zdgavd dxtrf gdtbfkac fk` `ospasd a ot ok f prapdr wfstd maktfokdr+ Prfksdr tbd maktfokdrs ta f :'cotdr jdfidr a
uctrfpurd wftdr "~5 maktfokdrs pdr : cotdrs a wftdr/+
1 trauJcdsBaatOkH"xoo/ ^cfmd tbd jdfidr ak f stor pcftd fk` stor+"xooo/ Mbfkhd tbd wftdr :”= togds f `fy ar :”= `+"xov/ Zdgavd tbd smfac`s rag tbd gac`s fk` rdpcfmd tbdg ok rdsb wftdr ar fk f``otoakfc `fy+ ]mfac`s wotbaut
rdokarmdgdkt wocc jdhok ta eaft wbdk tbd sfct os rdgavd`+"xv/ ]tard smfac`s ok uctrfpurd wftdr ok 4>'gc tujds ft 0 ¾M uktoc kdd`d` ar usd "mfk jd stard` ar :”5 gaktbs/ ar `ry
tbd smfac`s fk` stard tbdg `ry ft raag tdgpdrfturd+"xvo/ Xbdk kdd`d` ar usd# mut tbdg ok wftdr ft `dsord` `ogdksoaks fk` futamcfvd ta stdrocozd+ Xd suhhdst scomokh a tbd
tap fk` jattag cfydrs fk` `osmfr`okh tbdg# fs tbd surfmd bfs f siok tbft os cdss paraus+
1 trauJcdsBaatOkH
1 trauJcdsBaatOkHPraujcdsbaatokh f`vomd mfk jd auk` ok tj :+
"maktokud`/
tfJcd : | Praujcdsbaatokh tfjcd+
s pjg so
64 "soci pramdssokh/ ]oci `ads kat ossacvd ftdr 0 b
ok cotboug jrago`d "CoJr/
@o` yau fmmaukt ar tbd vacugd a CoJr wbdk prdpfrokh tbd ?+= G sacutoak1
O kat# f`` tbd fppraproftd fgaukt a CoJr+
@o` yau jaoc tbd mamaaks ok sa`oug mfrjakftd ar => gok1 O yau jaocd` ar f
sbartdr togd# yau gfy kdd` ta fccaw tbd soci ta ossacvd ok CoJr ar cakhdr+ Gfidkatd a tbd jaocokh togd fs jftmbds gfy vfry o tbd jaocokh togds frd odrdkt+
@o` yau paur tbd CoJr avdr tbd soci ojdrs1 O kat# yau wocc kdd` ta gox tbd
sacutoak ta dksurd maktfmt jdtwddk tbd soci fk` tbd CoJr sacutoak+ Jd surd ta
mavdr tbd jdfidr ta rd`umd dvfparftoak+
Mbdmi tbft tbd avdk os sdt ta 5> ¾M
CoJr fk` soci ojraok sacutoak os
kat fgjdr macard`
Mbfkhd ar mcdfk hcfsswfrd
:4F"oo/ "tujds/ ]oci wocc kat dvdkcy maft tbd
word fk` sdvdrfc jdf`s frd
prdsdkt fcakh tbd gfk`rdc
]oci os kat makmdktrftd` dkauhb# stard ft 0 ¾M ar f dw fys# mbdmi ot
pdroa`omfccy fs tbd soci wocc hdc o ot os stard` ar taa cakh
7/30/2019 Rockwood(2011)_Materials Fabrication From Bombyx Mori Silk Fibroin_Nature Protocols
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©:>66
KfturdFgdromf#Okm+Fccrohbtsrdsdrvd`+
pratamac
kfturd pratamacs | UAC+5 KA+6> | :>66 | 65:?
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]tdp :4E# rdpfrftoak a pB hdcs< 6> gok]tdp :4H# ^rdpfrftoak a soci ocgs< : `]tdp :4B# Hdkdrftokh gac`s fk` mfstokh a pfttdrkd` soci ocgs< 5 b ar hdkdrftokh gac`s# : ` ar mfstokh]tdp :4O# ^rdpfrftoak a gomraspbdrds "@A^M/< 0+4 `]tdp :4L# rdpfrftoak a gomraspbdrds< : ]tdp :4I# Dcdmtraspokkokh< 6 `
tfJcd : | Praujcdsbaatokh tfjcd "maktokud`/+
s pjg so
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O tbd prajcdg maktokuds# accaw tbd pratamac# mfrduccy fccawokh tbd jdf` ta ha
up fk` awk tbd gfk`rdc+ Fcsa# fccaw 6> s ar gdtbfkac trdftgdkt fk` => s ar
tbd tujd ta `ry jdtwddk cfydrs
Cfrhd hfps frd prdsdkt ak tbd
tujds
Jujjcds frd prdsdkt ok tbd soci sacutoak+ Jd mfrduc kat ta ok`umd jujjcds wbdk
popdttokh ar goxokh ok ^DA "o fppcomfjcd/+ @dhfs tbd sacutoak ok f vfmuug avdk
jdard usd
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spokkokh/
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bfs jdf`s fcakh ot
O tbd ojdr os kat ukoarg fs ot os jdokh wrfppd` frauk` tbd gfk`rdc# ot wocc
rdsuct ok fk ukdvdk tujd+ Jdf`s fcakh tbd hdc ojdr ok`omftd tbft tbd soci sacutoak
os kat makmdktrftd` dkauhb+ ]tard tbd sacutoak ft 0 ¾M ar f maupcd a fys jut
mbdmi ot pdroa`omfccy fs tbd soci wocc hdc o ot os stard` ar taa cakh
:4M"ov/ "vartdx hdcs/ Hdc wocc kat arg Uartdxokh togd fk fgpcotu`d gfy kdd ta jd f lustd dpdk okh ak tbd jftmb
a soci# fhd a tbd sacutoak fk` vacugd
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:4D"ooo/
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tbd sacutoak fk` vacugd+ Dcdmtrahdcftoak waris jdst ak soci sacutoaks tbft frd
fppraxogftdcy 6 wddi ac`
:4H"vo/ "tbok ocgs/ Eocgs wocc kat cot a a tbd
pcftd
Tsd kak”tossud mucturd”trdftd` osbds
:4L"ox/ "^UF
gomraspbdrds/
Pbd ocg wocc kat magpcdtdcy
`ossacvd ftdr => gok
]akomftd tbd sacutoak ar => s ft 64$ fgpcotu`d
:4C"o/#:4G"o/
"spakhds/
Xbdk sbotokh tbd sfct# tbdrd
frd vdry dw mrystfcs wotbok tbd
sozd rfkhd a oktdrdst
Pbd sodvd gfy jd mcahhd`+ Okvdrt tbd sodvd fk` try ta osca`hd pfrtomcds trfppd`
wotbok tbd gdsb
:4G"xo/ "BEO^'jfsd`
spakhds/
BEO^'jfsd` spakhds `ossacvd`
wbocd ok wftdr
Gdtbfkac wfs kat fjcd ta pdkdtrftd prapdrcy+ Zdpdft jut dxtdk` tbd gdtbfkac
trdftgdkt togd
:4G"xvo/ "BEO^'
jfsd` spakhds/
BEO^'jfsd` spakhds bfvd vao`s
ok tbdg
Pry pusbokh tbd smfac`s awk ar mdktrouhd tbdg jdard ryokh
7/30/2019 Rockwood(2011)_Materials Fabrication From Bombyx Mori Silk Fibroin_Nature Protocols
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©:>66
KfturdFgdromf#Okm+Fccrohbtsrdsdrvd`+
pratamac
65=> | UAC+5 KA+6> | :>66 | kfturd pratamacs
]tdp :4C# ^rdpfrftoak a fqudaus'jfsd` spakhds< 4 `]tdp :4G# ^rdpfrftoak a BEO^'jfsd` spakhds< 2 `
fktOmOpftd@ rdsuctsDxpdmtd` autmagds ar tbd vfroaus aptoaks `dsmrojd` ok tbd ^ZAMD@TZD frd suggfrozd` ok tbd OKPZA@TMPOAK+
::+ Cavdtt# G+ dt fc+ ]oci jraok gomratujds ar jcaa` vdssdc dkhokddrokh+Joagftdrofcs :2# 4:86”4:8? ":>>8/+
:=+ Cavdtt# G+C+# Mfkkozzfra# M+G+# Uuklfi'Kavfiavom# H+ % Ifpcfk# @+C+Hdc spokkokh a soci tujds ar tossud dkhokddrokh+ Joagftdrofcs :?#054>”0548 ":>>2/+
:0+ Cavdtt# G+C+# Zamiwaa`# @+K+# Jfrysbyfk# F+ % Ifpcfk# @+C+ ]ogpcdga`ucfr joardfmtars ar tossud dkhokddrokh< f systdg ar mbfrfmtdrozftoaka axyhdk hrf`odkts# bugfk gdsdkmbygfc stdg mdcc `odrdktoftoak# fk`
prdvfsmucfrozftoak+ Possud Dkh+ ^frt M Gdtba`s+ 65# 6454”648= ":>6>/+:4+ Iog# T+L+ dt fc+ ]trumturd fk` prapdrtods a soci by`rahdcs+
Joagfmragacdmucds 4# 825”8?: ":>>0/+
:5+ _umdc# P+# Mdjd# + % Ifpcfk# @+C+ Uartdx'ok`umd` okldmtfjcd soci jraokby`rahdcs+ Joapbys+ L+ ?8# :>00”:>4> ":>>?/+
:8+ Xfkh# R+V+# Icuhd# L+F+# Cdosi# H+H+ % Ifpcfk# @+C+ ]akomftoak'ok`umd`
hdcftoak a soci jraok ar mdcc dkmfpsucftoak+ Joagftdrofcs :?# 6>40”6>50":>>2/+
:2+ Cdosi# H+H+# Ca# P+L+# _umdc# P+# Cu# V+ % Ifpcfk# @+C+ Dcdmtrahdcftoak ar
pratdok f`bdsovds+ F`v+ Gftdr+ ::# 866”864 ":>6>/+:?+ _umdc# P+# Ialom# K+# Cdosi# H+H+# Ca# P+L+ % Ifpcfk# @+C+ Kak'dquocojroug
soci jraok f`bdsovds+ L+ ]trumt+ Joac+ 68># 0>5”06: ":>6>/+
=>+ Bagfkk# ]+ dt fc+ ]oci jraok fs fk arhfkom pacygdr ar maktraccd` `ruh`dcovdry+ L+ Maktrac Zdcdfsd 666# :6?”::8 ":>>5/+
=6+ Cfwrdkmd# J+@+# Gfrmbfkt# L+I+# ^ok rus# G+F+# Agdkdtta# E+H+ % Ifpcfk# @+C+
]oci cg joagftdrofcs ar markdf tossud dkhokddrokh+ Joagftdrofcs =>#6:??”6=>2 ":>>?/+
=:+ Lok# B+L+# ^fri# L+# Ufccuzzo# Z+# Mdjd# + % Ifpcfk# @+C+ Joagftdrofc cgs
a Jagjyx garo soci jraok wotb pacy"dtbycdkd axo`d/+ Joagfmragacdmucds 4#866”868 ":>>0/+
==+ Xfkh# R+ dt fc+ ]oci gomraspbdrds ar dkmfpsucftoak fk` maktraccd` rdcdfsd+
L+ Maktrac Zdcdfsd 668# =5>”=8> ":>>8/+=0+ Xfkh# R+V+# _umdc# P+# Cu# V+# Bu# R+ % Ifpcfk# @+C+ ]oci kfkaspbdrds fk`
gomraspbdrds rag soci!pvf jcdk` cgs ar `ruh `dcovdry+ Joagftdrofcs =6#
6>:4”6>=4 ":>6>/+
=4+ Lok# B+L+# Ero`roib# ]+U+# Zutcd`hd# H+M+ % Ifpcfk# @+C+ DcdmtraspokkokhJagjyx garo soci wotb pacy"dtbycdkd axo`d/+ Joagfmragacdmucds =#
6:==”6:=? ":>>:/+=5+ Co# M+# Udpfro# M+# Lok# B+'L+# Iog# B+L+ % Ifpcfk# @+C+ Dcdmtraspuk soci'
JG^': smfac`s ar jakd tossud dkhokddrokh+ Joagftdrofcs :8# =664”=6:0
":>>5/+=8+ Zamiwaa`# @+K+# Fioks# Z+D+# ^frrfh# O+M+# Xaa`bausd# I+F+ % Zfjact# L+E+
Mucturd ak dcdmtraspuk pacyurdtbfkd smfac`s `dmrdfsds ftrofc kftrourdtom
pdpto`d dxprdssoak jy mfr`oagyamytds ok votra+ Joagftdrofcs :?# 082=”08?6":>>2/+
=2+ Ifif`d# G+U+ dt fc+ Dcdmtrom dc` ok`umd` arodktftoak a pacygdr mbfoks ok
gfmrasmapomfccy fcohkd` dcdmtraspuk pacygdr kfkajdrs+ L+ Fg+ Mbdg+ ]am+ 6:?#:888”:82: ":>>8/+
=?+ Iog# T+L+# ^fri# L+# Iog# B+L+# Xf`f# G+ % Ifpcfk# @+C+ Pbrdd'`ogdksoakfc
fqudaus'`drovd` joagftdrofc smfac`s rag soci jraok+ Joagftdrofcs :5#:884”:824 ":>>4/+
0>+ Kfzfrav# Z+# Lok# B+L+ % Ifpcfk# @+C+ ^araus ='@ smfac`s rag
rdhdkdrftd` soci jraok+ Joagfmragacdmucds 4# 862”8:5 ":>>0/+06+ Zamiwaa`# @+K+ dt fc+ Okhrawtb a bugfk gdsdkmbygfc stdg mdccs okta
paraus soci pfrtomcd rdokarmd` soci magpasotd smfac`s< fk ok votra stu`y+
Fmtf Joagftdr+ 8# 600”646 ":>66/+0:+ \bfkh# R+B+# Zdfhfk# G+Z+ % Ifpcfk# @+C+ Dcdmtraspuk soci joagftdrofc
smfac`s ar rdhdkdrftovd gd`omokd+ F`v+ @ruh @dc+ Zdv+ 56# ?22”6>>5
":>>?/+0=+ Iog# B+L+# Iog# T+L+# Uuklfi'Kavfiavom# H+# Gok# J+B+ % Ifpcfk# @+C+
Okeudkmd a gfmraparaus pratdok smfac`s ak jakd tossud dkhokddrokh
rag jakd gfrraw stdg mdccs+ Joagftdrofcs :5# 000:”004: ":>>4/+00+ Bagfkk# ]+ dt fc+ Maktrac a ok votra tossud'dkhokddrd` jakd'coid
strumturds usokh bugfk gdsdkmbygfc stdg mdccs fk` paraus soci smfac`s+
Joagftdrofcs :2# 664:”665: ":>>8/+04+ Iog# B+L+ dt fc+ Jakd tossud dkhokddrokh wotb prdgokdrfcozd` soci smfac`s+
Jakd 0:# 6::5”6:=0 ":>>2/+
fmIkawcd@HGdkts Pbd futbars fmikawcd`hd tbd accawokh rdsdfrmbdrs ar tbdormaktrojutoaks ta tbdsd pratamacs< D+]+ Hoc# M+ Xottgdr# B+ L+ Iog# D+F+ rotmbfr`#R+ Xfkh# L+F+ Icuhd fk` E+ Agdkdtta+ Pbos wari wfs suppartd` jy tbd Kftoakfc Okstotutds a Bdfctb "^06 DJ>>:4:>‐Possud Dkhokddrokh Zdsaurmd Mdktdr/# tbd
Kftoakfc ]modkmd Eauk`ftoak fk` tbd For Earmd Aomd a ]modktoom Zdsdfrmb+
futBar maktrOJutOaks @+K+Z+# Z+M+^+# P+_+# R+X+ fk` G+C+C+ wratd tbd
gfkusmropt fk` @+C+I+ supdrvosd` fk` d`otd` tbd praldmt+
maGpdtOkH EOkfkmOfc Oktdrdsts Pbd futbars dmcfrd ka magpdtokh
eokfkmofc oktdrdsts+
^ujcosbd` akcokd ft bttp<!!www+kfturdpratamacs+mag!+
Zdprokts fk` pdrgossoaks okargftoak os fvfocfjcd akcokd ft bttp<!!www+kfturd+mag!rdprokts!ok`dx+btgc+
6+ Gurpby# F+Z+ % Ifpcfk# @+C+ Joagd`omfc fppcomftoaks a mbdgomfccy'ga`od` soci jraok+ L+ Gft+ Mbdg+ 6?# 500=”504> ":>>?/+
:+ Gurpby# F+Z+# Labk# +]+ % Ifpcfk# @+C+ Ga`omftoak a soci jraok usokh
`ofzakoug maupcokh mbdgostry fk` tbd ddmts ak bG]M pracodrftoak fk``odrdktoftoak+ Joagftdrofcs :?# :2:?”:2=2 ":>>2/+
=+ Xdki# D+ dt fc+ Pbd usd a sucakftd` soci jraok `drovftovds ta maktrac jok`okh#
`dcovdry fk` patdkmy a EHE': ok tossud rdhdkdrftoak+ Joagftdrofcs =6#60>=”606= ":>6>/+
0+ Udpfro# M+# Gftbdsak# @+# @ruggy# C+# Kfoi# Z+ % Ifpcfk# @+C+ ]urfmd
ga`omftoak a soci jraok wotb pacy"dtbycdkd hcymac/ ar fktof`bdsoak fk`fktotbragjatom fppcomftoaks+ L+ Joagd`+ Gft+ Zds+ F ?=f# 4?4”5>5 ":>6>/+
4+ ]af# ]+# GmMfrtby# G+J+# Hrakawomz# H+ % Ifpcfk# @+C+ Eukmtoakfcozd` soci'
jfsd` joagftdrofcs ar jakd argftoak+ L+ Joagd`+ Gft+ Zds+ 40# 6=?”602":>>6/+
5+ Udpfro# M+ % Ifpcfk# @+C+ ]oci fs f joagftdrofc+ ^rah+ ^acy+ ]mo+ =:#
??6”6>>8 ":>>8/+8+ Mrfoh# M+ % Zodidc# M+ Magpfrftovd frmbotdmturd a socis# jraus pratdoks
fk` tbdor dkma`okh hdkds ok oksdmts fk` spo`drs+ Magp+ Mbdg+ ^bysoac+J Joambdg+ Gac+ Joac+ 6==# 0?=”4>8 ":>>:/+
2+ Agdkdtta# E+H+ % Ifpcfk# @+C+ Kdw appartukotods ar fk fkmodkt gftdrofc+]modkmd =:?# 4:2”4=6 ":>6>/+
?+ Fctgfk# H+B+ dt fc+ ]oci'jfsd` joagftdrofcs+ Joagftdrofcs :0# 0>6”065 ":>>=/+6>+ Barfk# Z+C+ dt fc+ Ok votra `dhrf`ftoak a soci jraok+ Joagftdrofcs :5#
==24”==?= ":>>4/+
66+ ^fri# ]+B+ dt fc+ Zdcftoaksbops jdtwddk `dhrf`fjocoty a soci smfac`s fk`astdahdkdsos+ Joagftdrofcs =6# 565:”568: ":>6>/+
6:+ Gdokdc# C+ dt fc+ Pbd okefggftary rdspaksds ta soci cgs ok votra fk`
ok vova+ Joagftdrofcs :5# 608”644 ":>>4/+6=+ Xrfy# C+]+# Bu# R+ % Ifpcfk# @+C+ Ddmt a pramdssokh ak soci'jfsd`
joagftdrofcs< rdpra`umojocoty fk` joamagpftojocoty+ L+ Joagd + Gftdr+ Zds+ J
Fppc+ Joagftdr+ pujcosbd` akcokd# `ao<6>+6>>:!ljg+j+=6284 ":6 Lukd :>66/+60+ Iog# @+'B+ dt fc+ @ossacvfjcd cgs a soci jraok ar uctrftbok makargfc
joa'oktdhrftd` dcdmtrakoms+ Kft+ Gftdr+ ?# 466”468 ":>6>/+
64+ Dtodkkd# A+ dt fc+ ]at tossud fuhgdktftoak usokh soci hdcs< fk ok votra fk`ok vova stu`y+ L+ ^droa`akt+ 2># 624:”6242 ":>>?/+65+ Xfkh# _+ dt fc+ Ok vova `dhrf`ftoak a tbrdd'`ogdksoakfc soci jraok
smfac`s+ Joagftdrofcs :?# =064”=0:2 ":>>2/+68+ Gdokdc# C+ dt fc+ ]oci jfsd` joagftdrofcs ta bdfc mrotomfc sozd` dgur
`ddmts+ Jakd =?# ?::”?=6 ":>>5/+
62+ Bu# R+ dt fc+ Zdhucftoak a soci gftdrofc strumturd jy tdgpdrfturd'maktraccd`wftdr vfpar fkkdfcokh+ Joagfmragacdmucds 6:# 6525”65?5 ":>66/+
6?+ Bu# R+# Cu# V+# Ifpcfk# @+C+ % Mdjd# + Gomrapbfsd sdpfrftoak maktraccd`
jdtf'sbddt mrystfccozftoak iokdtoms ok jraus pratdoks+ Gfmragacdmucds 0:#:>8?”:>28 ":>>?/+
:>+ ]ukh# K+'_+ dt fc+ Ddmt a hfggf orrf`oftoak ak tbd strumturfc fk`
pbysoacahomfc prapdrtods a soci jraok+ Eaa` ]mo+ Joatdmbkac+ 62# ::2”:==":>>?/+
:6+ Ialtbukh# F+ dt fc+ Ddmt a hfggf rf`oftoak ak joa`dhrf`ftoak a
Jagjyx garo soci jraok+ Okt+ Joa`dtdroar+ Joa`dhrf`+ 5:# 028”0?> ":>>2/+
7/30/2019 Rockwood(2011)_Materials Fabrication From Bombyx Mori Silk Fibroin_Nature Protocols
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©:>66
KfturdFgdromf#Okm+Fccrohbtsrdsdrvd`+
pratamac
05+ Iog# B+L+ dt fc+ Jakd rdhdkdrftoak ak gfmraparaus fqudaus'`drovd` soci='@ smfac`s+ Gfmragac+ Joasmo+ 8# 50=”544 ":>>8/+
08+ Bagfkk# ]+ dt fc+ Mfrtocfhd'coid tossud dkhokddrokh usokh soci smfac`sfk` gdsdkmbygfc stdg mdccs+ Possud Dkh+ 6:# :8:?”:8=2 ":>>5/+
02+ Pohco# Z+]+ dt fc+ Magpfrftovd mbak`rahdkdsos a bugfk mdcc saurmds ok =@
smfac`s+ L+ Poss+ Dkh+ Zdhdk+ Gd`+ =# =02”=5> ":>>?/+0?+ Xfkh# _+\+# Jcfsoaco# @+L+# Iog# B+L+# Iog# B+]+ % Ifpcfk# @+C+ Mfrtocfhd
tossud dkhokddrokh wotb soci smfac`s fk` bugfk frtomucfr mbak`ramytds+
Joagftdrofcs :8# 00=0”000: ":>>5/+
4>+ Xfkh# _+\+# Iog# T+L+# Jcfsoaco# @+L+# Iog# B+L+ % Ifpcfk# @+C+ Ok votra mfrtocfhd tossud dkhokddrokh wotb =@ paraus fqudaus'`drovd` soci smfac`s
fk` gdsdkmbygfc stdg mdccs+ Joagftdrofcs :5# 8>2:”8>?0 ":>>4/+46+ Hbasb# ]+# Cfbf# G+# Gak`fc# ]+# ]dkhuptf# ]+ % Ifpcfk# @+C+ Ok votra
ga`dc a gdsdkmbygfc mak`dksftoak `urokh mbak`rahdkom `dvdcapgdkt+
Joagftdrofcs =># 54=>”540> ":>>?/+4:+ Gfukdy# L+Z+ dt fc+ Dkhokddrokh f`opasd'coid tossud ok votra fk` ok vova
utocozokh bugfk jakd gfrraw fk` f`opasd'`drovd` gdsdkmbygfc stdg mdccs
wotb soci jraok =@ smfac`s+ Joagftdrofcs :2# 4:2>”4:?> ":>>8/+4=+ Hoc# D+]+# ^fri# ]+B+# Gfrmbfkt# L+# Agdkdtta# E+ % Ifpcfk# @+C+ Zdspaksd
a bugfk markdfc jrajcfsts ak soci cg surfmd pfttdrks+ Gfmragac+
Joasmo+ 6># 550”58= ":>6>/+40+ ]adr# C+ dt fc+ ]oci'jfsd` dcdmtraspuk tujucfr smfac`s ar tossud'
dkhokddrd` vfsmucfr hrfts+ L+ Joagft+ ]mo+' ^acy+ D`+ 6?# 54=”550 ":>>2/+
44+ \bfkh# R+B+# Jfuhbgfk# M+J+ % Ifpcfk# @+C+ Ok votra dvfcuftoak a dcdmtraspuk soci jraok smfac`s ar vfsmucfr mdcc hrawtb+ Joagftdrofcs :?#::68”:::8 ":>>2/+
45+ \bfkh# R+B+ dt fc+ @ykfgom mucturd mak`otoaks ta hdkdrftd soci'jfsd`tossud'dkhokddrd` vfsmucfr hrfts+ Joagftdrofcs =># =:6=”=::= ":>>?/+
48+ Bausd# G+# ]fkmbdz# M+M+# Zomd# X+C+# ]amrftd# ]+ % Ifpcfk# @+C+ Mdrvomfc tossud
dkhokddrokh usokh soci smfac`s fk` bugfk mdrvomfc mdccs+ Possud Dkh+ ^frt F 65#:6>6”:66: ":>6>/+
42+ ]mbkdo`dr# F+# Xfkh# R+_+# Ifpcfk# @+C+# Hfrcomi# L+F+ % Dhcds# M+
Joaukmtoakfcozd` dcdmtraspuk soci gfts fs f tapomfc joafmtovd `rdssokh arfmmdcdrftd` wauk` bdfcokh+ Fmtf Joagftdr+ 4# :48>”:482 ":>>?/+
4?+ Xbfrrfg# ]+D+# \bfkh# R+B+# Ifpcfk# @+C+ % GmMfrtby# ]+ + Dcdmtraspuk socigftdrofc systdgs ar wauk` bdfcokh+ Gfmragac+ Joasmo+ 6># :05”:48
":>6>/+5>+ Gardfu# L+D+ dt fc+ Possud'dkhokddrd` jakd sdrvds fs f tfrhdt ar
gdtfstfsos a bugfk jrdfst mfkmdr ok f gausd ga`dc+ Mfkmdr Zds+ 58#
6>=>0”6>=>2 ":>>8/+56+ Xfkh# R+C+ dt fc+ F magpcdx =@ bugfk tossud mucturd systdg jfsd` ak
gfggfry stragfc mdccs fk` soci smfac`s ar ga`dcokh jrdfst
garpbahdkdsos fk` ukmtoak+ Joagftdrofcs =6# =?:>”=?:? ":>6>/+
5:+ Xfkh# R+C+ dt fc+ ^rdf`opamytds stogucftd `umtfc garpbahdkdsos fk`ukmtoakfc `odrdktoftoak a bugfk gfggfry dpotbdcofc mdccs ak =@ soci
smfac`s+ Possud Dkh+ ^frt F 64# =>28”=>?2 ":>>?/+5=+ ]ujrfgfkofk# J+ dt fc+ Possud'dkhokddrd` tbrdd'`ogdksoakfc ok votra ga`dcs
ar kargfc fk` `osdfsd` io`kdy+ Possud Dkh+ ^frt F 65# :2:6”:2=6 ":>6>/+
50+ Fctgfk# H+B+ dt fc+ ]oci gftrox ar tossud dkhokddrd` fktdroar mrumoftdcohfgdkts+ Joagftdrofcs :=# 06=6”0606 ":>>:/+
54+ Gardfu# L+D+# Jrfgaka# @+]+# Barfk# Z+C+# Ifpcfk# @+C+ % Fctgfk# H+B+
]dqudktofc joambdgomfc fk` gdmbfkomfc stogucftoak ok tbd `dvdcapgdkt a tossud'dkhokddrd` cohfgdkts+ Possud Dkh+ ^frt F 60# 6656”668: ":>>2/+
55+ Lofkh# R+V+ dt fc+ Gfk`ojucfr rdpfor ok rfts wotb prdgokdrfcozd` soci
smfac`s fk` JG^':'ga`od` jG]Ms+ Joagftdrofcs =># 04::”04=:":>>?/+
58+ \bfa# L+ dt fc+ Fpftotd'maftd` soci jraok smfac`s ta bdfcokh gfk`ojucfr
jar`dr `ddmts ok mfkokds+ Jakd 04# 468”4:8 ":>>?/+52+ ^rotmbfr`# D+G+# ]zyjfcf# M+# Jaosak# @+ % Ifpcfk# @+C+ ]oci jraok
dkmfpsucftd` paw`dr rdsdrvaors ar sustfokd` rdcdfsd a f`dkasokd+
L+ Maktrac Zdcdfsd 600# 64?”658 ":>6>/+5?+ ]zyjfcf# M+ dt fc+ Fktodpocdptom ddmts a soci'pacygdr jfsd` f`dkasokd
rdcdfsd ok iok`cd` rfts+ Dxp+ Kdurac+ :6?# 6:5”6=4 ":>>?/+
8>+ Xocz# F+ dt fc+ ]oci pacygdr'jfsd` f`dkasokd rdcdfsd< tbdrfpdutom patdktofc ar dpocdpsy+ Joagftdrofcs :?# =5>?”=565 ":>>2/+
86+ Xfkh# R+V+ dt fc+ Hrawtb fmtar hrf`odkts vof gomraspbdrd `dcovdry ok
joapacygdr smfac`s ar astdambak`rfc tossud dkhokddrokh+ L+ Maktrac
Zdcdfsd 6=0# 26”?> ":>>?/+