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  • IX73INSTRUCTIONS

    RESEARCH INVERTED MICROSCOPE

    This instruction manual is for the Olympus research inverted microscope model IX73.

    To ensure the safety, obtain optimum performance and to familiarize yourself fully with the use

    of this microscope, we recommend that you study this manual thoroughly before operating the

    microscope.

    Retain this instruction manual in an easily accessible place near the work desk for future reference.

    A X 8 1 5 7

  • In accordance with European Directive 2002/96/EC on Waste Electrical and Electronic Equip-

    ment, this symbol indicates that the product must not be disposed of as unsorted municipal

    waste, but should be collected separately.

    Refer to your local Olympus distributor in EU for return and/or collection systems available in

    your country.

  • 1 Intended use ...............................................................................................................................................................................................4

    2 Conformity of the System ................................................................................................................................................................5

    3 Handling Precautions..........................................................................................................................................................................6

    4 Maintenance and Storage ..............................................................................................................................................................6

    1 MODULE NOMENCLATURE .................................................................................................7

    2 NOMENCLATURE ..........................................................................................................................8

    3 TRANSMITTED LIGHT BRIGHTFIELD OBSERVATION PROCEDURE .12

    4 USING THE CONTROLS ..........................................................................................................14

    4-1 Power Supply Unit and Microscope Frame .......................................................................................14

    1 Turning Power On, Adjusting the Brightness .................................................................................................................14

    2 Light Path Selection ..............................................................................................................................................................................15

    3 Coded intermediate magnification changer IX3-CAS ....................................................................................................... 15

    4 Frame Fix Plate IX3-FP .......................................................................................................................................................................15

    5 Dust tray ..........................................................................................................................................................................................................16

    4-2 Focusing Block............................................................................................................................................................17

    1 Rotation Direction of the Coarse/Fine Adjustment Knobs ..........................................................17

    2 Adjusting the Coarse Adjustment Knob Tension .......................................................................................................17

    3 Detaching the Fine Adjustment Knob ..................................................................................................................................17

    4 Pre-focusing Lever .................................................................................................................................................................................17

    4-3 Stage ....................................................................................................................................................................................18

    1 Placing the Specimen ........................................................................................................................................................................18

    2 Moving the Specimen.........................................................................................................................................................................21

    3 Connecting the Grounding Wire ...............................................................................................................................................22

    IMPORTANT -- Be sure to read this section for safe use of the equipment. -- 1-6

  • 4-4 Observation Tube ......................................................................................................................................................23

    1 Adjusting the Interpupillary Distance ....................................................................................................................................23

    2 Adjusting the Diopter ...........................................................................................................................................................................23

    3 Using the Eye Shades ........................................................................................................................................................................24

    4 Mounting the Eyepiece Micrometer Disk ..........................................................................................................................24

    5 Selecting the Light Path of the Trinocular Tube ...........................................................................................................24

    6 Adjusting the Tilt (U-TBI90) .............................................................................................................................................................24

    4-5 Illumination Column (IX3-ILL) ..........................................................................................................................25

    1 Tilting the Illumination Column ...................................................................................................................................................25

    2 Mounting Filters........................................................................................................................................................................................26

    3 Using the Field Iris Diaphragm ...................................................................................................................................................27

    4 Adjusting the Condenser Height Adjustment Knob Tension ...................................................................................................................27

    5 Condenser refocusing stopper ..................................................................................................................................................27

    4-6 Condenser ......................................................................................................................................................................28

    1 Centering the Condenser ................................................................................................................................................................28

    2 Using the Aperture Iris Diaphragm .........................................................................................................................................30

    4-7 Oil- or Water-Immersion Objective ..........................................................................................................31

    1 Using Oil- or Water-Immersion Objective .........................................................................................................................31

    4-8 Objectives with Correction Collar ...............................................................................................................32

    5 OTHER OBSERVATION METHODS .................................................................................33

    5-1 Phase Contrast Observation ...........................................................................................................................33

    1 Phase Contrast Optical Elements and Applicable Objectives .......................................................................33

    2 Attaching the Phase Contrast Optical Elements ........................................................................................................34

    3 Centering the Phase Contrast Ring Slit ..............................................................................................................................36

    5-2 Differential Interference Contrast Observation ................................................................................37

    1 DIC Optical Elements, Applicable Objectives and DIC Sliders ......................................................................37

  • 2 Attaching the DIC Optical Elements ......................................................................................................................................38

    3 Attaching the Analyzer and DIC Slider ................................................................................................................................39

    4 Attaching the Polarizer (IX-LWPO) ...............................................