Embed Size (px)
Citation preview
Plant Physiology is planning a special issue devoted toReactive Oxygen Species in June 2006. All excellent paperscovering reactive oxygen species signaling and metabolism,oxidative stress homeostasis, and redox signaling research inplants will be considered.
Authors interested in contributing to this special issue shouldindicate this in their cover letter when submitting papers online at
http://submit.plantphysiol.org/. Manuscripts to be considered forthe special issue should be submitted no later than January 31, 2006.
For additional information, please contact Editor-in-Chief Donald Ort([email protected]) or Special Issue Editors Julia Bailey-Serres
([email protected]) and Ron Mittler ([email protected]).
Plant PhysiologyAnnounces a Reactive Oxygen Species Special Issue
The Open Access movement in scholarly publishing advocates that research content shouldbe freely available to all immediately upon publication. This approach has prompted publish-ers to examine the feasibility of a shift from traditional subscription-based (“user pays”)financial models to an “author-pays” model, in which some or all of the costs of publicationare typically borne by authors.
What does our author community think about Open Access? To gauge the plant sciencecommunity’s interest in this new approach to publishing and to help ASPB determine theviability of “author-pays” publishing models, the Society is conducting an 18-month OpenAccess “experiment.” Beginning with the December 2005 issues of The Plant Cell and PlantPhysiology, authors of articles accepted by the journals will be given the option to pay a sur-charge to make their online article free from the moment of publication to anyone withInternet access. The surcharge, which is in addition to the usual author charges, will be$1,000 (discounted to $500 if the author’s institution subscribes to the journal).
For more information, go to http://www.aspb.org/publications/openaccess.cfm or contactNancy Winchester, ASPB director of publications, at [email protected].
Plant Cell Lysis
www.piercenet.com/pper38j
The P-PER® Reagent Kit † (#89803) extractsmaximum active protein from stem, root, seed andleaves in 10 minutes without liquid nitrogen.
You can trust the experts at Pierce to know how to get max-imum protein extraction from your sample. We have beenthe protein people for over 50 years, providing the bestsolutions to extract, purify and quantify proteins from manytypes of cell lines and tissue.
Is your plant cell lysis leafing you out in the cold?
Fresh leaf tissue and seed were lysed and extracted according to the P-PER® Kit (Product # 89803)protocol, a competitor’s protocol and a literature-based (home brew) protocol. Samples were normalized(weight tissue/volume extract), resolved on a 10% Bis-Tris gel and stained with Imperial™ ProteinStain† (Product # 24615). Samples were also quantified using the BCA™ Protein Assay Kit, ReducingAgent Compatible (Product # 23250).
*The Competitor S kit is not recommended for dried seed.
Tel: 815-968-0747 or 800-874-3723 • Fax: 815-968-7316 • Customer Assistance E-mail: [email protected] the United States, visit our web site or call 815-968-0747 to locate your local Perbio Science branch office (below) or distributor
© Pierce Biotechnology, Inc., 2005. Pierce products are supplied for laboratory or manufacturing applications only.
BCA™, P-PER® and Imperial™ are trademarks of Pierce Biotechnology, Inc. † U.S. patents pending on P-PER® Technology, Imperial™ Protein Stain and Reducing Agent-compatible BCA™ Technology.
Belgium & Dist.:Tel +32 53 85 7184
China:Tel +86 10 8049 [email protected]
France:Tel 0800 50 82 15
Germany: Tel 0228 9125650
Hong Kong:Tel 852 2753 0686
The Netherlands: Tel 076 50 31 880
United Kingdom: Tel 0800 252185
Switzerland: Tel 0800 56 31 40
P-PER® Reagent Kit Highlights:
• Convenient – disrupts cells without harsh mechanical methods in 10 minutes
• Compatible – downstream applications include 1-D and 2-D gel electrophoresis,Western blotting, activity assays, and protein affinity purifications
• Quantifiable – use the BCA™ Protein Assay Kit, Reducing Agent Compatible†
• Ready-to-use – extract does not require filtration through cheesecloth or Miracloth
• Provides active proteins – extracted proteins are functional
New! Poppers™ Cell Lysis Solutions HandbookLog on to our web site or call us to requestyour FREE handbook (#1601234). Outsidethe U.S., contact Perbio Science or your local distributor.
200
976643
29
2014
6
3.6
0.02 0.016 0.009
Leaf
MW (kD)
Protein extracted (mg)/ total tissue weight (mg)
Pie
rce
Tobacco
Hom
e B
rew
Com
pet
itor
S
0.02 0.02 0.01
Pie
rce
Hom
e B
rew
Com
pet
itor
S
0.176 0.09
Pie
rce
Hom
e B
rew
0.056 0.046
Pie
rce
Hom
e B
rew
0.09 0.058 0.063
Pie
rce
Hom
e B
rew
Com
pet
itor
S
Corn Leaves Arabidopsis Soybean Corn Kernel
Seed*
G r a s p t h e P r o t e o m e ®
Joint Annual Meeting of the American Society of Plant Biologists
and the Canadian Society of Plant Physiologists
Société Canadienne de Physiologie Végétale
Hynes Convention Center
Boston, MassachusettsAugust 5-9, 2006
For more information: American Society of Plant BiologistsTelephone: 301-251-0560 • Fax: 301-279-2996 • E-Mail: [email protected] • Web Site: http://www.aspb.org/meetings/pb-2006
Photo Credit: Greater Boston Convention & Visitors Bureau
SYMPOSIAPlants Mitigating Global Change—Stephen P. Long, University of Illinois
Legumes: Genomes to Biology—Douglas R. Cook, University of California-Davis
Ion Channels and Cellular Signaling—Julian I. Schroeder, University of California-San Diego
Gibbs Medal Symposium: Genome Scale BiologyJoseph R. Ecker, The Salk Institute
President's Symposium: Plant Responses to the EnvironmentMichael F. Thomashow, Michigan State University
Plan
t Bio
logy
