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sigma.com/pcr ORDER: 800-325-30�0 TECHNICAL SERVICE: 800-325-5832�2
Custom Oligonucleotides
Sigma-Genosysoffersacompletelineofcustomoligonucle-otidesforyourresearch.Sigma-Genosyshasmanufacturingsitesaroundtheglobein10countries–Australia,Canada,France,Germany,India,Israel,Japan,Singapore,UKandUSA.Ourproprietaryplatforms,theAbacus™andtheARK™,provideunparalleledthroughput,capacityandqualityresult-inginaffordable,high-qualityoligos.
Sigma-GenosysisISO9001:2000registeredatourmanufacturingsitesinCanada,Germany,Israel,JapanandtheUSA.TheUKmanufacturingsiteisISO14001:1996registered.
Products and Servicesn StandardDNASynthesisandPurification
n SpecialtyPrimers
n LongOligosforArrays
n Real-timePCRProbes
n ModifiedOligos
n OligosforHigh-ThroughputApplications
n GeneSynthesis
n OligoDesignService
Features and Benefitsn Everyoligoisbar-codedforefficientandaccuratehandling
throughouttheproductionprocess
n Anadditionalviallabelisincludedforyourconvenience
n Everyoligoisdeprotectedanddesalted
n 100%QualityControlusingMALDI-TOFMassSpectrom-etry,ElectrosprayIonizationMassSpectrometry(ESI-MS),CapillaryElectrophoresis(CE)orPolyacrylamideGelElectrophoresis(PAGE)
n QualityAssurancedocumentationincludingmolecularmass,sequenceandyieldisincludedwitheveryorder
n Shippingwithin1–2daysforstandard(non-modified)oligos
n Mostmodifiedoligosshipwithin3-5workingdays
n Onlineordertrackingisavailableonourwebsite
n Technicalsupportstaffcanassistwitholigodesignandhelptotroubleshootdifficultassays
Custom Oligonucleotides from Sigma-Genosys
Purity Levels and Guaranteed Minimum Yields*�Purification� Recommended�Method� Applications� 0.025�µmole� 0.05�µmole� 0.2�µmole� 1.0�µmole� 10�µmole� 15�µmole
Desalting PCR,probing,mobilityshift, 3OD 5OD 12OD 40OD 400OD 600OD≤35bases arbitrary10-merapplications, 90µg 150µg 360µg 1,200µg 12,000µg 18,000µg hybridization
Cartridge Allaboveapplications,plus N/A 1OD 3OD 12OD N/A N/A(RPI) sequencing,DNAfinger- N/A 30µg 90µg 360µg N/A N/A≤50bases printing,cyclesequencing, RT-PCR,in situ hybridization, microsatellitepolymorphisms
HPLC Allaboveapplications, N/A 1OD 2.5OD 13OD 130OD 190OD(RPI) plusmutagenesis,S-oligos, N/A 30µg 75µg 390µg 3,900µg 5,700µg≤50bases large-scales,antisense†, andtriplehelix†
PAGE Allaboveapplications,plus N/A 0.5OD 1OD 5OD N/A N/A≤110bases genesynthesisandprimer N/A 15µg 30µg 150µg N/A N/A extension
* Guarantee is for non-modified oligos ≥20 bases; yields for shorter oligos will be proportionally less. S-oligos and other modified oligos will typically contain 30% and 40%, respectively, less yield than unmodified oligos.
† In combination with gel filtration.
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�3Our Innovation, Your Research — Shaping the Future of Life Science
Real-time PCR Probes
Sigma-Genosysoffersacompletelineofreal-timePCRchemistries.Ourbroadportfoliooffersavarietyofprobeandprimeroptionstosuityourindividualneeds.
Ourtechnicalsupportteamisalsoavailabletoassistyouwithyourprobeandprimerdesign.ThisserviceisofferedfreeofchargetoallSigma-Genosyscustomers.Simplycontactyourlocaltechnicalsupportofficeorcompletetheonlinerequestformformoreinformation.
Products and Servicesn Dual-labeledProbes
n MolecularBeacons
n LightCycler®Probes
n Scorpions®Probes
n LNA™Dual-labeledProbes
n LNAMolecularBeacons
nLNALightCyclerProbes
nProbeandPrimerDesignServices
Features and Benefitsn Fullydeprotectedanddesalted.PurifiedbyPAGEorRP-HPLC
n 100%QualityControlusingMALDI-TOFMassSpectrome-try,PolyacrylamideGelElectrophoresis(PAGE)orRP-HPLC
n Everyprobeisdelivereddriedinambertubestoensuredyeintegrity
n Onlineordertrackingisavailableonourwebsite
n Ourtechnicalserviceteamcanassistwithprobedesign
Shipping ScheduleLightCyclerprobes: 4to5workingdaysDual-labeledfluorogenicprobes: 5to6workingdaysMolecularBeaconsprobes: 5to6workingdaysScorpionsprobes: 7to10workingdays
Custom Dual-Labeled Probe & Molecular Beacon Formats
Guaranteed OD Yield
Approximate No. of nmoles*
Approximate No. of Reactions**
1 5 1,000
3 15 3,000
5 25 5,000
10 50 10,000
50 250 50,000
100 500 100,000
*1OD=5nmoles=30µgfora20-meroligo.Nmoleamountwillvarybasedonoligolength
**Numberofreactionsisbasedona25µlreactionsize,finalprobeconcentrationof200nM.
Cu
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sigma.com/pcr ORDER: 800-325-30�0 TECHNICAL SERVICE: 800-325-5832��
Custom OligonucleotidesCustomer/Technical Support
United States/North AmericaTel:[email protected]@sial.com
Australia/New Zealand 1800800097(Australia)0800936666(NewZealand)(+61)266228614
Austria: Tel:497329972950E-mail:[email protected]
Belgium: Tel:0800-14747E-mail:[email protected]
Canada(866)[email protected]
Czech Republic: Tel:+420246003200E-mail:[email protected]
Denmark: Tel:43565910E-mail:[email protected]
Finland: Tel:(09)3509250E-mail:[email protected]
France: Tel:0800779508E-mail:[email protected]
Germany+49/(0)7329/[email protected]
Greece: Tel:302109948010E-mail:[email protected]
Hungary: Tel:0612359054E-mail:[email protected]
India+91(80)[email protected]
Ireland: Tel:1800200888E-mail:[email protected]
Italy: Tel:800536373E-mail:[email protected]
Japan0133-75-7311(+81)[email protected]
Netherlands: Tel:08002288032E-mail:[email protected]
Norway: Tel:23176060E-mail:[email protected]
Poland: Tel:0618290100E-mail:[email protected]
Portugal: Tel:800202180E-mail:[email protected]
Spain: Tel:900101376E-mail:[email protected]
Sweden: Tel:020-350510E-mail:[email protected]
Switzerland: Tel:41817552650E-mail:[email protected]
United Kingdom/Europe 0800269016(UKOnly)+441440767000(Europe)[email protected]@europe.sial.com
Internetordersmaybesubmittedbyvisitingourwebsite:sigma.com/oligosOnlineordertrackingisavailableforordersplacedthroughtheInternet.
Fordetailsabouthowtoorderbyfaxore-mail,pleasecontactyourlocalcustomersupportteam.
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