Plant Molecular Biology 18: 601-602, 1992. © 1992 Kluwer Academic Publishers. Printed in Belgium.

Update section

Sequence

601

Nucleotide sequence of the chloroplast 23S rRNA gene from alder (Alnus incana)

Madeleine L6vesque and Douglas A. Johnson Ottawa-Carleton Institute of Biology, 30 George GlinskL Ottawa, Ontario, Canada KIN 6N5

Received and accepted 27 September 1991

Key words: Alnus incana, chloroplast, 23S rRNA

From an alder (Alnus incana) genomic library, we have isolated a clone containing the entire chloroplast rRNA operon. Restriction fragments spanning the 23S rRNA gene were subcloned into M 13mp 19, deletions were prepared [ 1 ] and both strands were sequenced [ 3]. The complete nucle- otide sequence (2811 bp) is shown in Fig. 1.

The 23 S coding region has a G + C content of 55~o and shows 98.3~o homology to the corre- sponding gene from Nicotiana tabacum [ 5 ], also a member of the subclass Magnoliopsida (dicots), and 95.3~ homology to the 23S rRNA gene of Zea mays [2] a member of the subclass Liliopsida (monocots). A 68 bp sequence thought to be spe- cific to monocots [4] is absent from the alder sequence.

Acknowledgements

This work was supported by a grant from the Natural Sciences and Engineering Research Council of Canada (NSERC). M.L. was a holder of an NSERC scholarship.

References

1. Dale RMK, McClure BA, Houchins JP: A rapid, single stranded cloning strategy for producing a sequential series of overlapping clones for use in DNA sequencing: appli- cation to sequencing the corn mitochondrial 18S rDNA. Plasmid 13:31-40 (1985).

2. Edwards K, Kossel H: The rRNA operon from Zea mays chloroplasts: nucleotide sequence of the 23 S rDNA and its homologies with E. coli 23S rDNA. Nucl Acids Res 9: 2853-2869.

3. Sanger F, Nicklen A, Coulson AR: DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74: 5463-5468 (1977).

4. Shimada H, Sugiura M: Fine structural features of the chloroplast genome: comparison of the sequenced chloro- plast genomes. Nucl Acids Res 19:983-995 (1991).

5. Shinozaki K, Ohme M, Tanaka M, Wakasugi T, Hayash- ida N, Matsubayashi T, Zaita N, Chunwongse J, Obokata J, Yamaguchi-Shinozaki K, Ohto C, Torazawa K, Meng BY, Sugita M, Deno H, Kamogashira T, Yamada K, Kusuda J, Takaiwa F, Kato A, Tohdoh N, Shimada H, Suglura M: The complete nucleotide sequence of the to- bacco chloroplast genome: its gene organization and ex- pression. EMBO J 5:2043-2049 (1986).

The nucleotide sequence data reported will appear in the EMBL, GenBank and DDBJ Nucleotide Sequence Databases under the accession number M76448.

602

TTCAAACGAG GAAAGGCTTA CGGTGGATAC CTAGGCACCC AGAGACGAGG AA~CGTAG TAAGCGACGA 70

AATGCTTCGG GGAGTTGAAA ATAAGCGTAG ATCCGGAGAT TCCCGAATAG GTCAACCTTT CAAACTGCTG 140

CTGAATCCAT GGGCAGGCAA GAGACAACCT AACGAACTGA AACATCTTAG TAGCCAGAGG AAAAGAAAGC 210

AAAAGCGATT CCCGTAGTAG CAGGCGAGCG AAATGGGAGC AC-CCTAAACC GTGAAAACGG GGTTGTGGGA 280

GAGCAATACA AGCGTCGTGC TC-CTAGGCGA AGCGGTGAAG TGCCGAACCC TAGATGGCTA GAGTCCAGTA 350

GCCGAAAGCA TCACTAGCTT ACGCTCTGAC CCGAGTAGCA TGGGGCACGT GGAATCCCGT GTGAATCAGC 420

AAGGACCACC TTGCAAGGCT AAATACTCCT GGGTGACCGA TAGTGAAGTA GTACCGTGAG GGAAGGGTGA 490

AA~GAACCCC CACCGGGGAG TGAAATAGAA CATGAAACCG TAAGCTCCCA AC-CAGTGGGA GGAGCCTGGG 560

GCTCTGACCG CGTGCCTGTT GAAGAATGAG CCGGCGACTC ATAGGCAGTG GCTTGGTTAA GGGAACCCAC 630

CGGAGCCGTA GCGAAAGCGA GTCTTCATAG CAATTGTC ACTGCTTATG GACCCGAACC TGGGTGATCT 700

ATCCATGACC AGGATGAAGC TTGC4~TGAAA CTAAGTGGAG GTCCGAACCG ACTGATGTTG AAGAATCAGC 770

GGATGAGTTG TGGTTAGGGG TGAAATGCCA CTCGAACCCA GAGCTAGCTG GTTCTCCCCG AAATGCGTTG 840

AGGCGCAGCA GTTGACTGGA CATCTAGGGG TAAAGCACTG TTTCGGTGCG GGCCGCGAGA GCGGTACCAA 910

ATCGAGGCAA ACTCTGAATA CTAGATATGA CCTCAAAATA ACAGGGGTCA AGGTCGGCCA GTGAGACGAT 980

GGGGGATAAG CTTCATCGTC GAGAGGGAAA CAGCCCGGAT CACCAGCTAA GGCCCCTAAA TGACCGCTCA 1050

GTGATAAAGG AGGTAGGGGT GCAGAGACAG CCAGGAGGTT TGCCTAGAAG CAGCCACCCT TGAAAGAGTG 1120

CGTAATAGCT CACTGATCOA GCGCTCTTGC C-CCGAAGATG AACGGGGCTA AGCGATCTGC CGAAGCTGTG 1190

GGATGTAAAA AGGCATCGGT AGGGGAGCGT TCCGCCTTAG GGGGAAGCAC CTGCGCGAGC AGCGGTAGAC 1260

GAAGCGGAAG COAGAATGTC GGCTTGAGTA ACGCAAACAT TGGTGAGAAT CCAATGCCCC GAAAACCTAA 1330

GGGTTCCTCC GCAAGGTTCG TCCACGGAGG GTGAGTCAGG GCCTAAGATC AGGCCGAAAG GCGTAGTCGA 1400

TGGACAACAG GTGAATATTC CTGTACTGCC CCTTGTTGGT CCCGAGGGAC GGAGGAGGCT AGGTTAGCC.G 1470

AAAGATGGTT ATCGGTTCAA GGACGCAAGG TGCCCCTGCT TTTTCAGGGT AAGAAGGGGT AGAGAAAATA 1540

CCTCGAGCCA ATGTTCGAGT ACCAGC4CGCT ACGGCGCTGA AGTAACCCAT GCCATACTCC CAGGAAAAGC 1610

TCGAACGACC TTCAACAAAA GGGTACCTGT ACCCGAAACC GACACAGGTA GGTAGGTAGA GAATACCTAG 1680

~CGAGA CAACTCTCTC TAAGGAACTC GGCAAAATAG CCCCGTAACT TCGC4~AGAAG GGGTGCCTCC 1750

TCACAAAGGA GGTCGCAGTG ACCAGGCCCG GGCGACTGTT TACCAAAAAC ACAGGTCTCC GCAAAGTCGT 1820

AAGACCATGT ATGGC.C4QCTG ACGCCTGCCC AGTGCCGGAA GGTCAAGGAA GTTGGTGACC TGATGACAGG 1890

GGAGCCAGCG ACCGAAGCCC CGGTGAACGG CGGCCGTAAC TATAACGGTC CTAAGGTAGC GAAATTCCTT 1960

GTCGGGTAAG TTCCGACCCG CACGAAAGGC GTAACGATCT GGGCACTGTC TCGGAGAGAG ACTCGGTGAA 2030

ATAGACATGT CTGTGAAGAT ~ACTACC TGCACCTGGA CAGAAAGACC CTATGAAGCT TCACTGTTCC 2100

CTGGGATTGG CTTTGGGCCT TTCCTGCGCA GCTTAGGTGG AGGGCGAAGA AGGCCTCCTT C C ~ C 2170

CCGAGCCGTC AGTGAGATAC CACTCTGGAA GAGCTAGAAT TCTAACCTTG TGTCAGGACC TACGGC, CCAA 2240

GGGACAGTCT CAGGTAGACA GTTTCTATGG GGCGTAGGCC TCCCAAAAGG TAACGGAGGC GTGCAAAGGT 2310

TTCCTCGGGC CAGACGGAGA TTGGCCCTCG AGTGCAAAGG CAGAAGGGAG CTTGACTGCA AGACCCACCC 2380

GTCGAGCAGG GACGAAAGTC GGCCTTAGTG ATCCGACGGT GCCGAGTGGA AGGGCCGTCG CTCAACGGAT 2450

AAAAGTTACT CTAGGGATAA CAGGCTGATC TTCCCCAAGA GCTCACATCG ACGGGAAGGT TTGGCACCTC 2520

GATGTCGGCT CTTCGCCACC TGGGGCTGTA GTATGTTCCA AGGGTTGGGC TGTTCGCCCA TTAAAGCGGT 2590

ACGTGAGCTG GGTTCAGAAC GTCGTGAGAC AGTTCGGTCC ATATCCGGTG ~GTTAG AGCATTGAGA 2660

GGACCTTTCC CTAGTACGAG AGGACCGGGA AGGACGCACC TCTGGTGTAC CAGTTATCGT GCCCACGGTA 2730

AACGCTGGGT AGCCAAGTGC GGAGCGGATA ACTGCTGAAA GCATCTAAGT AGTAAGCCCA CCCCAAGATG 2800

AGTGCTCTCC T 2811

Fig. 1. Nucleotide sequence of the chloroplast 23S rRNA gene from Alnus incana.