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A sputum culture is a test to detect and identify

bacteria or fungi (plural of fungus) that areinfecting the lungs or breathing passages.Sputum is a thick fluid produced in the lungs and

in the airways leading to the lungs. A sample of sputum is placed in a container with substancesthat promote the growth of bacteria or fungi. If nobacteria or fungi grow, the culture is negative. If organisms that can cause infection (pathogenicorganisms) grow, the culture is positive. The typeof bacterium or fungus will be identified with a

microscope or by chemical tests.



A sputum culture is done to:Detect and identify bacteria or fungi that

are causing an infection (such as pneumoniaor tuberculosis) of the lungs or the airwaysleading to the lungs. Symptoms of a lung infection may include difficulty breathing,pain when breathing, or a cough that

produces bloody or greenish brown sputum. Identify the best antibiotic to treat the

infection (sensitivity testing).Monitor treatment of an infection.



Do not use mouthwash before collecting a sputum

sample because it may contain antibacterialsubstances that could affect your results. If bronchoscopy will be used to collect your sputum

sample, your doctor will tell you how soon beforethe test to stop eating and drinking. you will need to stop eating at least 6 hours before

your test. You may be able to drink clear liquids, such as

water, coffee, or tea, up to 2 hours before the test.But be sure to follow the exact instructions your

doctor gives you.



the sputum sample is collected early in the morning before you eat or drink anything.If you wear dentures, you will need to remove thembefore collecting a sputum sample.

rinse your mouth with water, take a deep breath, andthen cough deeply to produce a sample of sputum.The health professional collecting the sample may

tap on your chest to help loosen the sputum in your lungs before you cough.If you still have trouble coughing up a sample, youmay be asked to inhale an aerosol mist to help you

cough.





Pulse oximetry is a procedure used to

measure the oxygen level (or oxygen saturation) in the blood. Itis considered to be a noninvasive,

painless, general indicator of oxygen delivery to the tissues (e.g.,finger, earlobe, or nose).



ÌOxygen in the air is breathed into the lungs. Theoxygen then passes into the blood where themajority of the oxygen attaches to hemoglobin (a

protein located inside the red blood cell) for transport in the bloodstream. The oxygenatedblood circulates to the tissues.

Ì Pulse oximetry technology utilizes the lightabsorptive characteristics of hemoglobin and thepulsating nature of blood flow in the arteries toaid in determining the oxygenation status in the

body.



Your physician will explain the procedure to youand offer you the opportunity to ask anyquestions that you might have about theprocedure.

Generally, fasting is not required before thetest.If a finger probe is to be used, you may be

asked to remove fingernail polish.Based upon your medical condition, your physician may request other specific

preparation.



A clip-like device called a probe(functions like a clothespin but does notpinch) will be placed on your finger or

earlobe. Alternatively, an adhesive probemay be placed on your forehead or finger.



The probe may be left on for continuous monitoring or only toobtain a reading.

Unless you are to have continuousmonitoring, the probe will be

removed after the test.



After the Procedure

You may resume your usual diet andactivities unless your physician advises you

differently.Generally, there is no special care following pulse oximetry. However, your physician

may give you additional or alternateinstructions after the procedure, depending on your particular situation.

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