Nanotech Retina

8/18/2019 Nanotech Retina

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Enginyeria Física 2015/2016 Nanotechnology 1

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NANOTECHNOLOGY

APPLIED TO THE RETINA

David Amat

16 de novembre de 2015Curs 2015-2016Nanotecnologia

Enginyeria Fsica


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REFERENCES

!asic "eference

• Intravitreal silicon-based quantum dots as neuroprotective factors in amodel of photoreceptor degeneration (Olson JL, Velez-Montoa !,Mandava ", #toldt $!% IOV# Jorunal &'&, )ug

#t$er "eferences

• "europrotective *+ect of hotoactive uantum .ots in rogressive!etinal hotoreceptor .egeneration (Olson JL, Velez-Montoa !, Mandava", #toldt $!% Journal of "anomaterials / Molecular "anotechnolog, &'0

Jul

• )rti1cial means for restoring vision (ar2ez 3ossain, Ian 4 #eetho,

)ndre2 $ 5ro2ning,4infried M )moa6u%, 5MJ Journal, &''7 8eb


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OUTLINE

• %ntroduction to retinal structure and biological be$aviour

• Aim of t$e e&'eriment

• Designing t$e e&'eriment

• "esults

• Discussions


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Introduction to retinal

structure

161 million people with visal impairment !2"2# o$ worl%wi%e poplation&

'etinal %isor%ers( %egeneration o$ neral cells !photoreceptors" )ipolar cells*& +rreversi)le e$$ect Electric stimlation as metho% o$ prevention


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Bioloical !e"a#iour

• Dis(s in rods contain r$odo'sin 'roteins

• "$odo'sin 'roteins contain 11-cis retinal

• )$otoisomeri*ation becuase of lig$t


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Bioloical !e"a#iour

• +$en lig$t stri(es, t$e transducin 'rotein is AC%.AED

• )DE transforms c/) to /)

• Closure of ion c$annels


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Bioloical !e"a#iour

• Absence of c/) ma(es t$e ion c$annels to C#E

• Negative 'otential inside

• "ods $y'er'olari*ed

• Electric negative current send to !%)#A" CE


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Ho$ e%es are tested &ERG'

• a-3ave due to '$otorece'tor negative 'otential res'onse

• b-3ave due to bi'olar and ganglion cells

• "od res'onse in DA"4 ADA)ED EE

• Cone res'onse in %/ ADA)ED EE


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Ai( o) t"e e*+eri(ent

hotoreceptor %egeneration

starts at 3r% wee o$ li$e

se o$ antm %ots as electrical stimlos generator


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Desinin t"e e*+eri(ent

32 eyes divided in 4 groups of 8 eyes each

ontrol ham 7 active 7 inactive

1 dose 2 doses 3 doses

4 eyes 2 eyes 2 eyes

• $am grou' received saline in7ection

• 8D in a colloidal a9ueous medium con7ugated 3it$ surfactant 'roteins

• 8D active: silicon 8D, gets e&cited by ;50 nm 3avelengt$ , and t$ird dose at 3ee( 6

• After ; 3ee(s, rats eut$ani*ed and $istological retina images analysis



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Results

,- Rod res+onse !% rou+s

• ig$ voltage res'onse in t$e active 8D?s eyes

analy*ed

• %nactive 8D?s eyes be$ave as t$e s$am grou'

• %nactive, s$am and control grou' s$o3 a 'oor

voltage res'onse at 3ee( ; com'ared to active 8D

$ig$ res'onse

ilicon

8ol%

ham

ontrol



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Results

.- /uantu( Dots (iration

• A: one $our after t$e in7ection 9uantum dots internal membrane retina

• !: i& $ours after t$e in7ection 9uantum dots migration across retina

• !y 2 day all 8D $ave crossed retina

• Nano'articles in7ected in eye are cleared by endot$elial system



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Results

0- Nu(!er o) in1ections

• "e'etitive 'rocess im'roving t$e results

1 in9ection

2 in9ections

3 in9ections ontrol



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Results

2- Retina "istolo%3 cell count

• A: ilicon 8D?s eye: all reinal layers 3ell-'reserved

• !: /old 8D?s eye: loose of '$otorece'tors in #N

• C: Control eye: very t$in layer



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Discussions

Electric

stimulation

%m'lants

C$i' retina

Dra3bac(s

)ar(inson

urgery

needed

"etinal

detac$ment

8uantum

Dots

No surgery

ore @8D

)rocess

re'eated

' remove

coating



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T"an4s )or %our attention

No$ it5s %our turn3

I) %ou "a#e an%

/ U E S T I O

N 1ust AS6

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Nanotech Retina