System Highlights

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In-VivoMSFXPRO

X-ray

Unmixed Fluorescent Signals

Reflectance

4-Layer Composite

Learn more. For more information,call 1-877-747-4357, exp. code 7.

Outside the U.S. call +1-203-786-5657.

The In-Vivo Multispectral FX PRO is designed for researchers who need to non-invasively image biologicalprocesses in small animal models. It provides unmatched versatility in small animal imaging with four imagingmodalities, fluorescence, luminescence, radioisotopic, and radiographic imaging, in one fully automated system.The MS FX PRO is now available with a new high speed X-ray head that enables researchers to rapidly captureX-ray images, ideal for co-registering functional and anatomical images.

TheMS FX PRO also features powerful excitation-based spectral unmixing that enables identification andseparation of multiple fluorophores and removal of autofluorescence background. TheMS FX PRO automaticallygenerates multispectral image “cubes” with spatially co-registered X-ray and white light images for improvedlocalization of biomarkers in vivo. This co-registration enables direct correlation of molecular signals to changes inbone structure, soft tissue or animal appearance.

Choose either the 10X zoom lens or our new, fast f/0.95 fixed lens option that is ideal for bioluminescent andradioisotopic imaging applications.

NEWhigh speed X-ray

and fixed lens

Unmatched Versatility� Four modalities in one system - fluorescence,luminescence, radioisotopic & high resolution X-ray

� Screening of both SPECT and PET probes withpatented high sensitivity radioisotopic screen

� 28 excitation filters excite any relevantfluorophores from the visible through the NIR

� Large 20 x 20 cm FOV for imaging 5 or moremice with zoom lens option

� 360 degree imaging with optional multimodalanimal rotation system (MARS)

Powerful Multispectral Analysis� Sophisticated software algorithms removeautofluorescence for improved signal to noiseand detection sensitivity

� Software saves and recalls excitation spectra tounmix multiple fluorophores

� Elegant visualization and rendering software forco-registering all modalities and multiplefluorophores

High resolution X-ray images captured injust 3 seconds

� New high speed 500 µA X-ray head� True microfocus X-ray source� Patented ultra-thin, ultra-uniform radiographicphosphor screen

� 3.3X geometric magnification stage standard

NEW! Enhanced sensitivity for bioluminescence,fluorescence and radioisotopic imaging

� Fast f/0.95 lens option� Powerful broad spectrum 400WXenonlight source

� Exclusive wide angle fluorescenceemission filters

Imaging of 18F-FDG in anormal mouse

NIRF tumor cells discovered in thelungs of a mouse

Contrast of kidneys and heart usingan i.v. X-ray contrast agent

Spectral unmixing of four fluorescentnanoparticles and co-registration with

autofluorescence signal.

Spectral unmixing of X-Sight 761 & 650nanospheres. Co-registration withX-ray and RGB reflectance images

In-VivoMultispectral FX PRO

Bruker Corporation

4 Research Drive, Woodbridge, CT 06525

Learn more. For more information,call 1-877-747-4357, exp. code 7.

Outside the U.S. call +1-203-786-5657.

www.bruker.com

Utilize the MS FX PRO for making fundamental biological discoveries and preclinical validation oftherapeutics and biomarkers. Image changes in signaling cascades in live animals over time todiscover how genetics, small molecules, and therapeutics affect these pathways. Validate anddiscover new molecular imaging probes and preclinical biomarkers — ideal for multi-functionalimaging agents including nanoparticles, peptides, antibodies and more.

Bruker’s pre-clinical imaging systems are not licensed to perform certain optical imaging applications that involve the in vivoimaging inmammals of (i) genetically expressed bioluminescent or fluorescent protein or (ii) conjugates of cells and light generatingmolecules, such applications are covered by patents owned or controlled by Caliper Life Sciences, Inc. Such patents include thefollowing: U.S. Patents Nos. 5,650,135; 6,217,847; 7,198,774; 6,649,143; 6,939,533; 6,916,462; 6,923,951; 6,890,515; 6,908,605;5,824,468; 6,638,752; 6,737,245 and 6,867,348; U.S. Patent Application No. 11/818,208; European Patent No. 0861093 andEuropean Patent Application No. 991246406; Japanese Patent Nos. 3786704 and 3786903; Canadian Patent No. 2237983;Singapore Patent No. 53708; Hong Kong Patent No. 1018747; and Chinese Patent No. 951980068.

©Bruker CorporationPrinted in USA December 2012

CAMERA DETECTIONMODES

CCD Interline CCD Luminescence

Pixel Density 2048 x 2048 pixels Multiwavelength Fluorescence

Cooling -29°C absolute Radioisotopic

Lens 10X zoom, ƒ/2.5 or X-Ray

Fast, ƒ/0.95 fixed lens EXPOSUREMODES

ILLUMINATION Single Capture

Source 400W Xenon Multiple Capture

Fluorescence Automated 29-position selectable Progressive Exposure

multiwavelength epi-illumination, Time Lapse Exposure

410 - 760 nm— includes 28 COMPUTER INTERFACE

excitation filters Ethernet

400Watt Xenon illumination source OPERATING SYSTEMS

White Light White light epi-illumination and Windows XP andWindows 7

white light transillumination Macintosh OS X

EMISSION FILTERS DIGITAL X-RAY

Automated selectable wide angle Resolution Zoom lens: ≥ 25 lp/mm (nominal)

filters, 535 - 830 nm – includes 6 Fixed lens: ≥ 19 lp/mm (mag stage)

emission filters Energy Range Approximately 20-45 kVp

PERFORMANCE Maximum Current 500 µA

Field of View Zoom: 2 x 2 cm to 20 x 20 cm or Spot Size < 60 µm

Fixed lens: 13.8 x 13.8 cm Target Material Tungsten

Optical Resolution Zoom: 10 µm/pixel (maximum) Window Filtration Beryllium

Fixed lens: 67 µm/pixel Cone of Illumination > 33 degrees

Data Acquisition 16-bit single capture Field of Illumination Zoom lens: 20 X 20 cm or

n-bit data acquisition Fixed lens: 13.8 x 13.8 cm

Binning 1x2, 2x2, 1x4, 2x4, 4x4, 1x8, 2x8, INSTRUMENT

4x8, 8x8, 16x16 Approx. Dimensions 41 x 24 x 38 in

Read Noise < 7-rms (nominal) (l x w x h) 104 x 61x 97 cm

Dynamic Range > 4 OD Approx. Net Weight 313 lbs, 142 kg

Dark Current Noise ≤ 5x10-5 e/pixel/s

System Specifications

In-Vivo SystemsA complete line of benchtop systems forhigh resolution optical, radioisotopic andX-ray imaging

Innovation with IntegrityPreclinical Imaging

One of themost respected brands in scientific instrumentation, Bruker Corporation is a $1.5 billion global company that serves researchers in a broad rangeof industries including life sciences, clinical research, pharmaceutical, biotechnology, material science andmore.With a focus on preclinical research,Bruker provides themost innovative technologies for small animal imaging includingmagnetic resonance imaging (MRI), magnetic particle imaging (MPI),optical, X-ray, nuclear (PET, SPECT), and X-ray computer tomography (micro CT). By offering themost expansive collection of imagingmodalities, theBruker imaging portfolio becomes unparalleled in themarket offering you themost complete and diversified choice for the advancement of small animalpreclinical research.

In the area of high resolution optical and X-ray, wewere the first to offer the combination of fourmodalities—luminescence, fluorescence, radioisotopic,and X-ray—in a single system for preclinical research.We offer four automated In-Vivo imaging systems in our classic benchtop configuration: the entrylevel In-Vivo F PRO, our unique DXS PRO digital X-ray system, themid-level In-Vivo FX PRO, and our full featured In-VivoMS FX PRO. They all provideumatched versatility, superior image quality and streamlinedworkflow. For those researchers who require greatersensitivity for bioluminescence applications, we nowoffer a fast f/0.95 fixed lens option as well as highresolution, high speed digital x-ray for all of ourIn-Vivo systems.

In-Vivo Imaging Systems

� Unmatched imaging versatility with rapidmultimodal acquisitions� Rapid imaging of fluorescent & radioisotopic labeled molecules in combination

with luminescent and X-ray imaging

� Automatic co-registration of optical images with high resolution X-ray images

� High throughput screening of both PET and SPECT probes

� Four imaging modalities, one system� Fluorescence, luminescence, radioisotopic and radiographic imaging modalities

available in one system

� Co-register signals from any imaging modality

� 360° imaging with multimodal animal rotation system (MARS)� Never miss a critical signal because of animal positioning

� Maximize sensitivity by imaging your animal at just the right angle

� Very high resolution and superior image quality� Exclusive wide angle emission filters enhance detection sensitivity and image quality

� Unique imaging geometry improves image clarity by providing a flat focal plane

� Advanced 4.2 megapixel camera and cooled CCD technology

� Fast, 3-second exposures with truemicrofocus X-ray source� New high-speed X-ray head

� Patented ultra-thin, ultra-uniform radiographic screen

� Enhanced performance for bioluminescent, fluorescent& radioisotopic imaging� New fast f/0.95 lens option

� Powerful 400WXenon illumination source

� Fast, convenient workflow� Single click acquisition of complex imaging protocols

� High throughput imaging of multiple animals in all modalities

� Live preview facilitates easy subject positioning and focusing

� User friendly, state-of-the-art software suite� Automated Region of Interest tools make image analysis fast, easy and quantitative

� Sophisticated software algorithms remove autofluorescence

� World-class service, training and technical support� Choice of extended service packages to maximize instrument performance

� Expert training for users at all skill levels

This is what makes our In-Vivo systems great:

NIR fluorescent imaging oflung tumors

High resolution X-ray of amurine cranium

Simultaneous imaging of fourfluorophores

Complete digital imaging solutionsWhen you invest in a Bruker In-Vivo imaging system, you are doingmore than just purchasing an instrument. You are getting an entireteam dedicated to making sure your research is successful. Fromour powerful, easy-to-use software, to our knowledgeable andprofessional technical support team, to customizable trainingprograms, you are getting an entire turnkey solution to meet yourmolecular imaging needs.

Superior molecular imaging softwareOurMolecular Imaging Software optimizes your system’s perform-ance and your productivity with integrated image acquisition,quantitative analysis and image databasing capabilities. Thenavigational structure features workflow-driven tool palettes forimproved ease of use.

� Powerful, easy-to-use capture & visualization

� Simple, automated analysis and reporting

� Intuitive user interface for managing workflow

� Unified platform for managing all images

� Superior data protection and image integrity

� Quantitative measures of light and X-ray density

Worldwide service and technical supportWewant your research programs to succeed, so we support youwith a comprehensive suite of service, training and technical supportprograms that are second to none.We help you protect yourinvestment by offering:

� A comprehensive warranty, backed by an expert service team, soyou are covered from day one

� A choice of service packages from basic to premium andpreventive maintenance

� A range of technical support options including phone support andremote access support

� Application support by our team of PhD scientists

� Problem solving assistance by our imaging experts and highlyresponsive world-wide support team

Training programs for users at all levelsWe help you achieve more by offering training programs that arecustom designed to meet your specific imaging and applicationneeds. Select from cost-effective options for users at all levels: frombasic introductory skills to in-depth techniques for advanced users.From one-on-one instruction to a full classroom, on-site or on-line —it’s your choice.

In-Vivo DXS PRO In-Vivo F PRO In-Vivo FX PRO In-Vivo MS FX PRO

Excitation Filters

Filter Positions NA 29 29 29

Filters Provided NA 14 14 28

Standard Filters NA 410 nm - 760 nm 410 nm - 760 nm 410 nm - 760 nm

Additional Filters Available NA 390 nm - 770 nm 390 nm - 770 nm 390 nm - 770 nm

Emission Filters

Wide Angle NA Yes Yes Yes

Filter Positions NA 4 4 4

Filters Provided NA 6 6 6

Standard Filters NA 535 nm - 830 nm 535 nm - 830 nm 535 nm - 830 nm

Additional Filters Available NA 440 nm - 850 nm; custom 440 nm - 850 nm; custom 440 nm - 850 nm; custom

Specifications

Standard & Optional Filters

Detection of green fluorescentdyes in vivo

High resolution NIRFand X-ray imaging

X-ray of mouse pup paw(with geometric magnification)

Swarm plate of fluorescentPseudomonas aeruginosa

Camera Performance

CCD Monochrome interline CCD Imaging Area 2 x 2 cm to 20 x 20 cm, 10X zoom or 13.8 cm, fixed lens

Pixel Density 2048 x 2048 pixels Resolution 10 µm/pixel (max.), 10X zoom or 67 µm/pixel, fixed lens

Lens 10X zoom, ƒ/2.5 or fixed ƒ/0.95 lens Pixel Size 7.4 µm

Illumination Data Acquisition 16-bit single capture n-bit data acquisition

Source 400W Xenon Dark Current Noise ≤ 5x10-5 e/pixel/sec (@ -29°C absolute)

Fluorescence Selectable multi-wavelength, epi-illumination Read Noise < 7 e-rms (nominal)

White Light Epi-illumination Dynamic Range > 4.0 orders of magnitude

Transillumination Binning 1x2, 2x2, 1x4, 2x4, 4x4, 1x8, 2x8, 4x8, 8x8, 16x16

Digital X-ray Animal Management

Resolution ≥ 25 lp/mm (contact) (zoom lens) Animal management chambers

≥ 19 lp/mm (with mag stage ) (fixed lens option) Automated animal heater (optional for DXS PRO)

Energy Range 20-45 kVp Atmospheric ports

Maximum Current 500 µA Optional anesthesia system

Spot Size < 60 µm Software

Target Material Tungsten 3 licenses of MI and Multiplex software** (PC only)

Window Filtration Beryllium **Multiplex software not included with DXS PRO

Exposure Modes 1 license of Multispectral software (MS FX PRO only)

Single Capture 0.175 sec–100 min (X-ray min 1.2 sec) Optional bone density software (not available for F PRO)

Multiple Capture 0.175 sec–100min, 32 accumulations max System Requirements

X-ray minimum 1.2 sec Computer PC and monitor supplied standard

Progressive Exposure 0.175 sec–100 min per frame, minimum increment = 1 sec Interface Ethernet

X-ray minimum 1.2 sec Operating Systems Windows XP, Windows 7 and Macintosh OS X*

Time Lapse Exposure 0.175 sec–100 min per exposure, minimum interval = 0.675 sec *DXS PRO is not Macintosh compatible.

X-ray minimum 1.2 sec

Select your in vivo imaging solution from four high-performance benchtop systemsBruker offers a selection of In-Vivo Imaging Systems so you can choose one that best meets your particular imaging needs. Eachsystem provides exceptional resolution, sensitivity and image quality for accurate quantification of your data.

Each system can now be configured with a fast f/0.95 lens that is ideal for bioluminescent and radioisotopic probes.

In-Vivo DXS PROThe DXS PRO System is ideal for X-ray imaging of smallanimals, plants, and more. It features a state-of-the-artradiographic phosphor screen that generates images withoutstanding 25 line pair per millimeter resolution contact.With our new fast 500 µA X-ray head whole animal X-raysare acquired in just 3 seconds. 10X continuous zoomprovides the flexibility to optimally image a variety ofspecimen sizes and zoom in on features of interest. The DXSPRO comes as a full energy calibrated system allowing fortrue quantification of your X-ray, and enabling bone densitysoftware. The DXS PRO also features automated controlsand filters for simplified workflow.

In-Vivo MS FX PROFor researchers who want to non-invasively image biologicalprocesses in small animal models, theMS FX PRO is a greatchoice. It combines any of four imagingmodalities (fluorescence,luminescence, radioisotopic, and radiographic) into one systemalong with powerful fluorescence spectral unmixing that removesautofluorescence and enables simultaneous imaging of multiplefluorophores. The first multispectral imaging systemwith a truemicrofocus X-ray head, it delivers high resolution X-ray imagesfor co-registration in just 3 seconds. The patented radioisotopicimaging screen allows researchers to screen both PET and SPECTprobes onmultiple animals. The optional MARS system enablesresearchers to image amouse from any angle using any of thefour imagingmodalities. Finally, our fast f/0.95 lens enables highspeed imaging of bioluminescence imaging of multiple animals.

In-Vivo FX PROThe In-Vivo FX PRO combines high sensitivity opticalmolecular imaging, radioisotopic imaging and highresolution digital X-ray to deliver precise anatomicallocalization of molecular and cellular biomarkers. Precisionautomation simplifies complex multimodal imagingprotocols and takes sensitivity, throughput, and ease ofuse to an entirely new level. For researchers whoseapplications focus on bioluminescence imaging of multipleanimals, we now offer a fast f/0.95 fixed lens option.

In-Vivo F PROThe compact In-Vivo F PROmakes an ideal entry-level systemfor researchers whose imaging requirements are expandingbeyond gels, blots and plates and into the study of diseasestates in vivo. Economical yet versatile, the F PRO provides highperformance optical molecular imaging of NIR fluorescent,radioisotopic and luminescent labels in small animals, a featurefurther enhanced with the new f/0.95 lens option. The F PROcan be easily upgraded to include X-ray and multispectralimaging as your research requirements evolve.

NEW!

Learn moreFor more information, to request pricing, an in-lab demo, or toplace an order, call 1-978-667-9580, Option 4.Or visit us online at

www.bruker.com

Product Selection Chart

Bruker Corporation • 15 Fortune Drive • Billerica, MA 01821Printed in U.S.A. ©Bruker Corporation February 2014

Spectral unmixing ofNIR probes

Spectral unmixing of livefluorescent-labeled bacteria

X-ray of mouse kneejoint (zoomed)

Three fluorescent imagesco-registered on X-rayimage of a mouse

18F Radioisotopic image ofFDG co-registered withwhite light image

Imaging capabilities include:

Instrument Modalities Features

Fluorescence Luminescence Radioisotopic Radiographic(X-ray) Automation Spectral

UnmixingMARS

Compatible

In-Vivo DXS PRO � � �

In-Vivo F PRO � � � �

In-Vivo FX PRO � � � � � �

In-Vivo MS FX PRO � � � � � � �

More optical/X-ray and nuclear small animal imaging systems from BrukerIn addition to our classic benchtop In-Vivo systems, we also offer the new In-Vivo Xtreme, a highly innovative tower system thatoffers a choice of back- or front-illuminated camera, and Albira, our revolutionary PET/SPECT/CT system that fits in virtually any lab.

Ask your Bruker representative for details.

Get seven imaging modalities in two instrumentswith the new In-Vivo Xtreme and Albira.

Ask us aboutAlbira andXtreme!

About Bruker CorporationBruker Corporation, a $1.5 billion public company with 6,200 employees worldwide, is aglobal technology andmarket leader in magnetic resonance imaging (MRI), magneticparticle imaging (MPI) and X-ray micro computer tomography (micro CT) andmore.With the addition of high resolution In-Vivo optical/X-ray and Albira PET, SPECT andCT systems, the Bruker preclinical imaging portfolio becomes unparalleled in the market,offering you the most diversified choice of imaging modalities for small animalpreclinical research.