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Molecularbiology Molecularbiology Green Fluorescent Protein

Molecularbiology Green Fluorescent Protein. Alba, the fluorescent bunny

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MolecularbiologyMolecularbiology

Green Fluorescent Protein

Alba, the fluorescent bunnyAlba, the fluorescent bunny

Alba, the fluorescent bunnyAlba, the fluorescent bunny

• "Alba", the green fluorescent bunny, is an albino rabbit.

• Alba is not green all the time.• It only glows when illuminated with the correct

light.• When (and only when) illuminated with blue light

(maximum excitation at 488 nm), she glows with a bright green light (maximum emission at 509 nm).

GFP - Other ApplicationsGFP - Other Applications

GFP Transgenic MiceGFP Transgenic Mice

Toes of newborn mice

Pancreatic tissue

Aequorea victoria (jellyfish)Aequorea victoria (jellyfish)

Green Fluorescent ProteinGreen Fluorescent Protein

E. coliE. coli

cells carrying and expressingthe gfp gene of A. victoria

wild type colonies

How to do it?How to do it?

?

Applications of CloningApplications of Cloning

PharmacyPharmacy

MedicineMedicine

FoodFood

AgricultureAgriculture

EnvironmentalEnvironmentalcleanupcleanup Basic researchBasic research

RegulationsRegulationsSafety & HealthSafety & Health

CloningCloning

Ethical concernsEthical concerns

Acceptance of genetic engineeringAcceptance of genetic engineering

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74

76

26

crops &food

pharmacy &medicine

data given in % Pro Contra

24

74

76

26

crops &food

pharmacy &medicine

data given in % Pro Contra

Source: Akademie für Technikfolgenabschätzung / Baden-Württemberg, 1998