Molecular Cytogenetics

.Molecular Cytogenetics Edited by

Barbara Ann Hamkalo and

John Papaconstantinou Biology Division Oak Ridge National Laboratory Oak Ridge, Tennessee

PlenU111 Press • New York-London

Library of Congress Cataloging in Publication Data

Main entry under tide:

Molecular cytogenetics

"Proceedings of ehe twenty-sixth annual Biology Division research con­ference held April 9-12, 1973 in Gadinburg, Tennessee; ... sponsored by the Biology Division of the Oak Ridge National Laboratory."

Includes bibliographies. 1. Molecular genetics-Congresses. 2. Cytogenetics-Congress. I. Hamkalo,

Barbara Ann, ed. II. Papaconstantinou, lohn, ed.lII. United States. National Laboratory, Oak Ridge, Tenn. Biology Division. [DNLM: 1. Cytogenetics­Congresses. QH605 OUm 1973] QH426.M64 575.2'1 73-18008 ISBN 978-1-4615-7481-1 ISBN 978-1-4615-7479-8 (eBook) DOI 10.1007/978-1-4615-7479-8

Proceedings of the Twenty-Sixth Annual Biology Division Research Conference, held April 9-12,197 3, in Gatlinburg, Tennessee.

Dr. Hamkalo's present address is the Department of Molecular Biology and Biochemistry, University of California at lrvine, lrvine, California 92664.

© 1973 Plenum Press, N ew Y ork Softcover reprint ofthe hardcover 1st edition 1973

A Division of Plenum Publishing Corporation 227 West 17th Street, New York, N.Y. 10011

United Kingdom edition published by Plenum Press, London A Division of Plenum Publishing Company, Ltd. Davis House (4th Floor), 8 Scrubs Lane, Harlesden, London, NWIO 6SE, England

All rights reserved

No part of this publication may be reproduced in any form without written permission from the publisher

FOREWORD

This volume represents the Proceedings of the Twenty-Sixth Annual Biology Division Research Conference held April 9-12 , 1973 in Gatlinburg, Tennessee. The subject of the symposium was Molecular Cytogenetics and the aim of the meeting was to bring together researchers interested in problems of chromosome organi­zation, activity and regulation in prokaryotes and eukaryotes. Cytological, biochemical and genetic approaches to these questions were included since the collective information gained fr om these disciplines provides an integrated approach to genome structure and function.

The meeting was sponsored by the Biology Division of the Oak Ridge National Laboratory*. It would not have been possible with­out the interest and cooperation of the organizing committee under the chairmanship of O. L. Miller, Jr. Special thanks are due to the chairmen and speakers for making this volume possible and to Dr. Waldo Cohn for his assistance in editing. Preparation of the completed volume was due in large part to the efforts of Sandra Vaughan of the Biology Division.

August, 1973

The Editors

Barbara Ann Hamkalo John Papaconstantinou

* Operated by the Union Carbide Corporation for the U.S. Atomic Energy Commission.

v

CONTRIBUTORS

ALBERTS, B., Department of Biochemical Sciences, Princeton University, Princeton, New Jersey

AMENSON, C. S., Division of Biology, California Institute of Technology, Pasadena, California

BAKKEN, A. H., Department of Biology, Yale University, New Haven, Connecticut

BALSAMO, J., Laborat6rio de Oncologia Experimental, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, Brasil

BERNARDI, G., Laboratoire de Genetique Moleculaire, Institut de Biologie Moleculaire, Paris, France

BIRD, A. P., Department of Biology, Yale University, New Haven, Connecticut

BIRNSTIEL, M. L., Institute for Molecular Biology, University of Zurich, Zurich, Switzerland

BLATTNER, F. R., McArdle Laboratory for Cancer Research, University of Wisconsin, Madison, Wisconsin

BRENTANI, R., Laborat6rio de Oncologia Experimental, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, Brasil

BRESCHKIN, A., Department of Molecular Biology, Vanderbilt University, Nashville, Tennessee

BRITTEN, R. J., Division of Biology, California Institute of Technology, Pasadena, Cali£ornia and Kerckhoff Marine Laboratory, Corona del Mar, California

BRZEZINSKA, M., Departments of Genetics and Biochemistry, University of Washington, Seattle, Washington

BUTTERWORTH, P. H. W., Department of Biochemistry, University College, London, U.K.

~i

CONTRIBUTORS

CALLAN, H. G., Zoology Department, The University, St. Andrews, Scotland

CASE, M. E., Department of Zoology, University of Georgia, Athens, Georgia

CASHEL, M., Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland

CHESTERTON, C. J., Biochemistry Department, University College London and King's College London, U.K.

DANEHOLT, B., Department of Histology. Karolinska Institutet, Stockholm. Sweden

DAVIDSON, E. H., Division of Biology. California Institute of Technology, Pasadena, California

DE POMERAI, D. I .• Biochemistry Department. University College London and King's College London. U.K.

EHRLICH, S. D •• Laboratoire de Genetique Moleculaire. Institut de Biologie Moleculaire, Paris. France

FLINT. S. J •• Biochemistry Department. University College London and King's College London. U.K.

GALAU, G. A., Division of Biology, California Institute of Technology. Pasadena, California

GALL. J. G .• Department of Biology. Yale University. New Haven, Connecticut

GEORGIEV. G. P .• Institute of Molecular Biology, Academy of Sciences of the U.S.S.R .• Moscow

GILES. N., Department of Zoology. University of Georgia. Athens, Georgia

GOLDBERG, R. B., Division of Biology. California Institute of Technology. Pasadena, California

GRAESSMANN. A .• Institut fUr Molekularbiologie und Biochemie. Freie UniversitHt, Berlin

GRAESSMANN, M .• Institut fUr Molekularbiologie und Biochemie. Freie UniyersitHt. Berlin

CONTRIBUTORS

GRAHAM, D. E., Division of Biology, California Institute of Technology, Pasadena, California and Kerckhoff Marine Laboratory, Corona del Mar, California

ix

GRUNSTEIN, M., Department of Medicine, Stanford University School of Medicine and Veterans Administration Hospital, Palo Alto, California

HALL, B. D., Department of Genetics, University of Washington, Seattle, Washington

HALLICK, R. B., Departments of Biochemistry and Biophysics, University of California Medical Center, San Francisco, California

HAMKALO, B. A., Biology Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee

HAUSMANN, R., Institut fUr Biologie III der Universitgt, Freiburg, West Germany

HAYES, S., McArdle Laboratory for Cancer Research, University of Wisconsin, Madison, Wisconsin

HILL, R. J., Department of Zoology, The University, St. Andrews, Scotland

HOLLENBERG, C. P., Departments of Genetics and Biochemistry, University of Washington, Seattle, Washington

HOUGH, B. R., Division of Biology, California Institute of Technology, Pasadena, California

JACOBSON, J. W., Department of Zoology, University of Georgia, Athens, Georgia

JEANTEUR, PH., Laboratoire de Biochimie-C.R.L.C., Hoptial Saint­Eloi, Montpellier, France

KEDES. L., Department of Medicine. Stanford University School of Medicine and Veterans Administration Hospital, Palo Alto, California

LARA, F. J. S., Department of Biochemistry. Institute of Chemistry, Sao Paulo, Brasil

LE TALAER, J. Y., Unite de Biochimie, Institut Gustave Roussy, Villejuif. France

x CONTRIBUTORS

MAHLER, H. R., Department of Chemistry, Indiana Uhiversity, Bloomington, Indiana

MARQUES, N., Laborat6rio de Oncologia Experimental, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, Brasil

MAUNDRELL, K. G., Department of Zoology, The University, St. Andrews, Scotland

MILLER, O. L, JR., Biology Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee

MOSIG, G., Department of Molecular Biology, Vanderbilt University, Nashville, Tennessee

MYIASHITA, M., Laborat6rio de Oncologia Experimental, Faculdade de Medicina, Universidade de Sao PaUlo, Sao Paulo, Brasil

NEUFELD, B., Division of Biology, California Institute of Technology, Pasadena, California and Kerckhoff Marine Laboratory, Corona del Mar, California

PARDUE, M. L., Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts

PERRY, R. P., The Institute for Cancer Research, Fox Chase Center for Cancer and Medical Sciences, Philadelphia, Pennsylvania

REINESS, G., Department of Biological Sciences, Columbia University, New York, New York

ROCHAIX, J. D., Department of Biology, Yale University, New Haven, Connecticut

RUTTER, W. J., Department of Biochemistry and Biophysics, University of California Medical Center, San Francisco, California

SCHEDL, P., Department of Biochemistry, Stanford University School of Medicine, Palo Alto, California

SCHULTZ, L. D., Departments of Genetics and Biochemistry, University of Washington, Seattle, Washington

SMITH, M. J., Division of Biology, California Institute of Technology, Pasadena, California

STOLF, A. M. S., Laborat6rio de Oncologia Experimental, Faculdade de Medicine, Universidade de Sao Paulo, Sao Paulo, Brasil

CONTRIBUTORS xi

SWIFT, H., Department of Biology, University of Chicago, Chicago, Illinois

SZYBALSKI, W., McArdle Laboratory for Cancer Research, University of Wisconsin, Madison, Wisconsin

THIERY, J. P., Laboratoire de GenetiQue Moleculaire, Institut de Biologie Moleculaire, Paris, France

WEINBERG, E. S., Department of Biology, Johns Hopkins University, Baltimore, Maryland

YANG, H. L., Department of Biological Sciences, Columbia University, New York, New York

ZUBAY, G., Department of Biological Sciences, Columbia University, New York, New York

CONTENTS

CRAPI'ER PAGE

1. As the Conference Opens . . . . . . . . . . . . . .. 1

2.

C. A. Thomas, Jr.

Sequence Organization in Animal DNA's ... E. H. Davidson, B. R. Hough, C. S. Amenson, Britten, D. E. Graham, B. R. Neufeld, M. J. R. B. Goldberg and G. A. Galau

R. J. Smith,

3. DNA Replication in the Chromosomes of Eukaryotes H. G. Callan

4. The Mechanism of Gene Amplification in Xenop~ laev-iA O'ocytes . . . . . . . .. .... A. P. Bird, J. D. Rochaix and A. H. Bakken

5. Repetitive DNA in V~o~ophi,ta J. G. Gall

9

31

59

6. Evolution of 9S mRNA Sequences . . . . 75

7.

M. L. Birnstiel, E. S. Weinberg and M. L. Pardue

A New Approach to the Study of Nucleotide Sequences in DNA's . . . . . . . . G. Bernardi, S. D. Ehrlich and J. P. Thiery

8 . On the Structure of Pre-mRNA and t he Transcriptional Unit in Eukaryotes G. P. Georgiev

9. Purification of Individual Histone mRNA's and Evolut ion of Their Sequences and Sizes M. Grunstein, P. Schedl and L. Kedes

10 . Re iteration of DNA Coding for Collagen Me ssenger

95

101

115

RNA. . . . . . . . . . . . . . . . . . 125 R. Brentani, N. Marques, J. Balsamo, L. Wang, M. Myiashita and A. M. S. Stolf

xiii

xiv

CHAPTER

ll. On the Role of Poly A Se~uences in mRNA Metabolism . R. P. Perry

12. Nonhistone Proteins of the Newt Germinal Vesicle R. J. Hill, K. G. Maundrell and H. G. Callan

13. The Giant RNA Transcript in a Balbiani Ring of Ch£nonomU6 ~entan6 . . . . . . . . . . . B. Daneholt

CONTENTS

PAGE

133

147

155

14. Studies on the Template Specificities of Eukaryotic DNA-Dependent RNA Polymerases ~n v~o 167 S. J. Flint, D. I. de Pomerai, C. J. Chesterton and P. H. W. Butterworth

15. Genetic Autonomy of Mitochondrial DNA H. R. Mahler

16. Involvement of RNA in the Process of Puff Induction in Polytene Chromosomes A. Graessmann, M. Graessmann and F. J. S. Lara

17. Stimulation of RNA Polymerase III by Histones and

181

209

Other Polycations •••• • . • . . . • • 217

18.

B. D. Hall, M. Brzezinska, C. P. Hollenberg and L. D. Schultz

A Transcription Complex from Chloroplasts of Euglena g~a~ . . . . . . . . . . . . . . R. B. Hallick and W. J. Rutter

227

19. Studies on the Replication of DNA . • . . • . • .• 233 B. Alberts

20. Regulation of Bacterial Genes •..•••• 253 G. Zub~, H. L. Yang, G. Reiness and M. Cashel

21. Host-Dependent Gene Expression of Bacteriophages T3 and T7 • . . . . . • • • • • . • • • • • 263 R. Hausmann

22. Synthesis of RNA Primer for Lambda DNA Replication Is Controlled by Phage and Host . • • • •• 277 s. H~es and W. Szybalski

CONTENTS

CHAPTER

23. Interaction in DNA Replication of, the Gene 32 and ~II Products of Phage T4 with the dna C product of

xv

PAGE

the Host ................... 285 G. Mosig and A. Breschkin

24. Some Considerations Regarding the Structure and Function of Promoter-Operator Regions . . . . . F. R. Blattner

25. Structure of Sigma-Dependent Binding Sites of E. eo~ RNA Polymerase to Phages A, T5 and T7 DNA IS. • • • • • • • •••••••••

Ph. Jeanteur and J. Y. Le Talagr

26. Genetic Regulation of Quinate-Shikimate Catabolism

293

301

in NeUhohpo~ ~ahha . . . . . . . . . . . . . . . 309 N. H. Giles, M. E. Case and J. W. Jacobson

27. Ultrastructural Aspects of Genetic Activity 315 B. A. Hamkalo, O. L. Miller, Jr. and A. H. Bakken

28. Molecular Cytogenetics; A Symposium Smmnary . . . 325 H. Swift

INDEX