12
MICROSCOPE COMPONENTS FOR TRANSMITTED LIGHT APPLICATIONS Specifications and equipment are subject to change without any notice or obligation on the part of the manufacturer. June 2007. ©1997-07 NIKON CORPORATION En Printed in Japan (0706-03)T Code No. 2CE-MUZH-3 This brochure is printed on recycled paper made from 40% used material. NIKON CORPORATION Parale Mitsui Bldg., 8, Higashida-cho, Kawasaki-ku, Kawasaki, Kanagawa 210-0005, Japan phone: +81-44-223-2167 fax: +81-44-223-2182 http://www.nikon-instruments.jp/eng/ NIKON INSTRUMENTS INC. 1300 Walt Whitman Road, Melville, N.Y. 11747-3064, U.S.A. phone: +1-631-547-8500; +1-800-52-NIKON (within the U.S.A.only) fax: +1-631-547-0306 http://www.nikonusa.com/ NIKON INSTRUMENTS EUROPE B.V. P.O. Box 222, 1170 AE Badhoevedorp, The Netherlands phone: +31-20-44-96-222 fax: +31-20-44-96-298 http://www.nikon-instruments.com/ NIKON INSTRUMENTS (SHANGHAI) CO., LTD. CHINA phone: +86-21-5836-0050 fax: +86-21-5836-0030 (Beijing office) phone: +86-10-5869-2255 fax: +86-10-5869-2277 (Guangzhou office) phone: +86-20-3882-0552 fax: +86-20-3882-0580 NIKON SINGAPORE PTE LTD SINGAPORE phone: +65-6559-3618 fax: +65-6559-3668 NIKON MALAYSIA SDN. BHD. MALAYSIA phone: +60-3-78763887 fax: +60-3-78763387 NIKON INSTRUMENTS KOREA CO., LTD. KOREA phone: +82-2-2186-8410 fax: +82-2-555-4415 NIKON CANADA INC. CANADA phone: +1-905-625-9910 fax: +1-905-625-0103 NIKON FRANCE S.A.S. FRANCE phone: +33-1-45-16-45-16 fax: +33-1-45-16-00-33 NIKON GMBH GERMANY phone: +49-211-9414-0 fax: +49-211-9414-322 NIKON INSTRUMENTS S.p.A. ITALY phone: +39-55-3009601 fax: +39-55-300993 NIKON AG SWITZERLAND phone: +41-43-277-2860 fax: +41-43-277-2861 NIKON UK LTD. UNITED KINGDOM phone: +44-20-8541-4440 fax: +44-20-8541-4584 NIKON GMBH AUSTRIA AUSTRIA phone: +43-1-972-6111-00 fax: +43-1-972-6111-40 NIKON BELUX BELGIUM phone: +32-2-705-56-65 fax: +32-2-726-66-45

MICROSCOPE COMPONENTS FOR TRANSMITTED …MICROSCOPE COMPONENTS FOR TRANSMITTED LIGHT APPLICATIONS Specifications and equipment are subject to change without any notice or obligation

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Page 1: MICROSCOPE COMPONENTS FOR TRANSMITTED …MICROSCOPE COMPONENTS FOR TRANSMITTED LIGHT APPLICATIONS Specifications and equipment are subject to change without any notice or obligation

MICROSCOPE COMPONENTSFOR TRANSMITTED

LIGHT APPLICATIONS

Specifications and equipment are subject to change without any notice or obligation on the part of the manufacturer. June 2007. ©1997-07 NIKON CORPORATION

EnPrinted in Japan (0706-03)T Code No. 2CE-MUZH-3 This brochure is printed on recycled paper made from 40% used material.

NIKON CORPORATIONParale Mitsui Bldg., 8, Higashida-cho, Kawasaki-ku,Kawasaki, Kanagawa 210-0005, Japanphone: +81-44-223-2167 fax: +81-44-223-2182 http://www.nikon-instruments.jp/eng/

NIKON INSTRUMENTS INC.1300 Walt Whitman Road, Melville, N.Y. 11747-3064, U.S.A.phone: +1-631-547-8500; +1-800-52-NIKON (within the U.S.A.only) fax: +1-631-547-0306http://www.nikonusa.com/

NIKON INSTRUMENTS EUROPE B.V.P.O. Box 222, 1170 AE Badhoevedorp, The Netherlandsphone: +31-20-44-96-222 fax: +31-20-44-96-298http://www.nikon-instruments.com/

NIKON INSTRUMENTS (SHANGHAI) CO., LTD.CHINA phone: +86-21-5836-0050 fax: +86-21-5836-0030(Beijing office) phone: +86-10-5869-2255 fax: +86-10-5869-2277(Guangzhou office) phone: +86-20-3882-0552 fax: +86-20-3882-0580

NIKON SINGAPORE PTE LTDSINGAPORE phone: +65-6559-3618 fax: +65-6559-3668

NIKON MALAYSIA SDN. BHD.MALAYSIA phone: +60-3-78763887 fax: +60-3-78763387

NIKON INSTRUMENTS KOREA CO., LTD.KOREA phone: +82-2-2186-8410 fax: +82-2-555-4415

NIKON CANADA INC.CANADA phone: +1-905-625-9910 fax: +1-905-625-0103

NIKON FRANCE S.A.S.FRANCE phone: +33-1-45-16-45-16 fax: +33-1-45-16-00-33

NIKON GMBHGERMANY phone: +49-211-9414-0 fax: +49-211-9414-322

NIKON INSTRUMENTS S.p.A.ITALY phone: +39-55-3009601 fax: +39-55-300993

NIKON AGSWITZERLAND phone: +41-43-277-2860 fax: +41-43-277-2861

NIKON UK LTD. UNITED KINGDOM phone: +44-20-8541-4440 fax: +44-20-8541-4584

NIKON GMBH AUSTRIA AUSTRIA phone: +43-1-972-6111-00 fax: +43-1-972-6111-40

NIKON BELUXBELGIUM phone: +32-2-705-56-65 fax: +32-2-726-66-45

Page 2: MICROSCOPE COMPONENTS FOR TRANSMITTED …MICROSCOPE COMPONENTS FOR TRANSMITTED LIGHT APPLICATIONS Specifications and equipment are subject to change without any notice or obligation

32

System DiagramContentsSystem Diagram ..............................

CFI60 ObjectivesCFI Achromat Seriesfor Brightfield/Phase Contrast (DL)............

CFI Achromat 4xCFI Achromat 10x / CFI Achromat DL 10xCFI Achromat LWD 20x / CFI Achromat LWD DL 20xCFI Achromat 40x / CFI Achromat DL 40xCFI Achromat 60xCFI Achromat 100x Oil / CFI Achromat DL 100x OilCFI Achromat LWD DL 20x FCFI Achromat LWD 40x C / CFI Achromat LWD DL 40x C

CFI Plan Achromat Seriesfor Brightfield/Phase Contrast (DL) ............

CFI Plan Achromat UW 1xCFI Plan Achromat UW 2xCFI Plan Achromat 4xCFI Plan Achromat 10x / CFI Plan Achromat DL 10xCFI Plan Achromat 20x / CFI Plan Achromat DL 20xCFI Plan Achromat 40x / CFI Plan Achromat DL 40xCFI Plan Achromat 40x NCGCFI Plan Achromat 50x OilCFI Plan Achromat 100x Oil / CFI Plan Achromat DL 100x OilCFI Plan Achromat 100x NCG

CFI Plan Fluor Seriesfor Brightfield/Phase Contrast (DLL) ..........

CFI Plan Fluor 4xCFI Plan Fluor 10x / CFI Plan Fluor DLL 10xCFI Plan Fluor 20x / CFI Plan Fluor DLL 20xCFI Plan Fluor 40x / CFI Plan Fluor DLL 40xCFI Plan Fluor 40x OilCFI Plan Fluor 60x CCFI Plan Fluor 100x Oil / CFI Plan Fluor DLL 100x OilCFI Plan Fluor 100x Oil with iris diaphragm

CFI Plan Fluor Series for Phase Contrast (DL)....CFI Plan Fluor DL 4xCFI Plan Fluor DL 10x

CFI Plan Fluor ELWD Seriesfor Brightfield/Phase Contrast (DM/DLL) ........

CFI Plan Fluor ELWD 20x C / CFI Plan Fluor ELWD DM 20x CCFI Plan Fluor ELWD 40x C / CFI Plan Fluor ELWD DM 40x CCFI Plan Fluor ELWD 60x C / CFI Plan Fluor ELWD DLL 60x C

Multi-Immersion Objective for Brightfield .....CFI Plan Fluor 20x MI

CFI Plan Apochromat Seriesfor Brightfield/Phase Contrast (DM) .........

CFI Plan Apochromat 2xCFI Plan Apochromat 4xCFI Plan Apochromat 10xCFI Plan Apochromat 20x / CFI Plan Apochromat DM 20xCFI Plan Apochromat 40x C / CFI Plan Apochromat DM 40x CCFI Plan Apochromat 40x Oil / CFI Plan Apochromat DM 40x OilCFI Plan Apochromat 60x C / CFI Plan Apochromat DM 60x CCFI Plan Apochromat 60x Oil / CFI Plan Apochromat DM 60x OilCFI Plan Apochromat 60x WICFI Plan Apochromat 100x Oil / CFI Plan Apochromat DM 100x OilCFI Plan Apochromat 100x NCG Oil

CFI S Fluor Series..........................................CFI S Fluor 10xCFI S Fluor 20xCFI S Fluor 40x CCFI S Fluor 40x OilCFI S Fluor 100x Oil

Water Dipping Series....................................CFI Fluor 10x WCFI Fluor 40x WCFI Fluor 60x W

CFI HMC Seriesfor Hoffmann Modulation Contrast® ..............

CFI HMC 10xCFI HMC 20x FCFI HMC LWD 40x C

2nd Objective Lens Unit ...............................Markings on CFI60 Objectives........................

Condensers....................................C-C Abbe Condenser, Dry TypeC-C Achromat CondenserAchromat Swing-out CondenserC-C Achromat /Aplanat CondenserLWD Achromat CondenserC-C Low Power CondenserDark Field Condenser, OilDark Field Condenser, DryC-CEL Expander Lens (for darkfield)

Illumination Systems ................Lamphouse HMX-3BLamphouse HMX-4B, built-in back mirrorLamphouse HMXMercury Lamp Socket S 100W for HMX-3BHalogen Lamp Socket 100W/HMXC-FC Epi-Fl Collector LensQuartz Epi-Fl Collector LensCollector Lens/QH100WC-LA HMX Lamphouse AdapterHMX Connecting Adapter SB-X1 (Bayonet to Screw)HMX Connecting Adapter SB-X2 (Screw to Bayonet)

Eyepiece Tubes ..............................Y-TB Binocular TubeY-TE Ergonomic Binocular TubeY-TF Trinocular Tube “F” UWY-TT Trinocular Tube “T” UW

Intermediate Tubes .......................Y-FL Epi-Fl AttachmentY-IDP Double Port

Eyepieces......................................CFI 10x with diopter adjustmentCFI 10x M Photomask with diopter adjustmentCFI 12.5x with diopter adjustmentCFI 15x with diopter adjustment

Nosepiece......................................V-NA Motorized Sextuple NosepieceV-ND MG-DIC Intelligent Sextuple NosepieceC-N Sextuple NosepieceC-ND Sextuple DIC Nosepiece

CCTV Camera Adapters .............V-T Photo AdapterY-T TV Tube for C-Mount Adapter AC-Mount TV Adapter ARelay Lens 1x IC-Mount TV AdapterENG-Mount TV AdapterZoom LensC-Mount Zooming AdapterENG-Mount Zooming AdapterC-Mount TV Adapter 0.45xC-Mount TV Adapter 0.6xENG-Mount TV Adapter 0.45x (for Trinocular Tube “F”, “T”)ENG-Mount TV Adapter 0.6x (for Trinocular Tube “F”, “T”)C-Mount TV Adapter VM 2.5xC-Mount TV Adapter VM 4x

22~23

21

20

20

19

18~19

171615

15

14

13

11~12

10

10

9

8~9

6~7

4~5

3

Darkfield Condenser (Oil)

C-CEL Expander Lens

C-C Abbe CondenserC-C Abbe Condenser(Dry)

C-C Achromat CondenserC-C Achromat Condenser Achromat Swing-out Condenser

C-C Achromat/Aplanat Condenser

LWD Achromat Condenser

C-C Low Power Condenser

Condensers

Darkfield Condenser (Dry)

Mercury LampSocket S 100W Lamphouse HMX-3B

Lamphouse HMX-4Bwith Built-in Back Mirror

C-FC Epi-Fl Collector LensQuartz Epi-Fl Collector LensCollector Lens/QH 100W

C-LA HMX Lamphouse Adapter HMX Connecting Adapter SB-X1 (Bayonet to Screw Mount)

HMX Connecting Adapter SB-X2 (Screw to Bayonet Mount)

Lamphouse HMXHalogen Lamp

Socket 100W/HMX

Illumination Systems

C-NSextuple Nosepiece

V-NDMG-DIC IntelligentSextuple Nosepiece

V-NAMotorized Sextuple Nosepiece

C-ND DICNosepiece

CFI60 Objectives

Nosepieces

Y-IDP Double Port

Y-FL Epi-Fl Attachment

Intermediate Tubes

Intermediate Tubes

Y-TT Trinocular Tube "T" UW Y-TF Trinocular Tube "F" UW Y-TB Binocular Tube Y-TE Ergonomic Binocular Tube

Eyepiece TubesY-T TV Tubefor C-Mount Adapter A

CFI 10Xwith Diopter Adjustment

CFI 10XMPhotomask with

Diopter AdjustmentCFI 15X

with Diopter AdjustmentCFI 12.5X

with Diopter Adjustment

Eyepieces

ENG-Mount TV Adapter*1*2 0.45X, 0.6X

ENG-Mount TV Adapter

ENG-Mount Zooming Adapter

Relay Lens 1X I

V-T Photo Adapter

C-Mount Zooming Adapter

Zoom Lens

C-Mount TV Adapter

Relay Lens 1X I

C-Mount TV Adapter 0.45X, 0.6X

C-Mount TV Adapter A

C-Mount TV Adapter VM2.5X

C-Mount TV Adapter A

C-Mount TV Adapter VM4X

C-Mount TV Adapter A

CCTV Camera AdaptersENG-Mount CCTV Camera C-Mount CCTV Camera

*1: Use the dedicated 0.45X adapter for the double port sub-port. The 0.6X adapter cannot be used with the double port sub-port.

*2: ENG-mount CCTV adapter 0.45x is a 38-bayonet mount type; other ENG-mount CCTV adapters are 48-bayonet mount types.

Page 3: MICROSCOPE COMPONENTS FOR TRANSMITTED …MICROSCOPE COMPONENTS FOR TRANSMITTED LIGHT APPLICATIONS Specifications and equipment are subject to change without any notice or obligation

ø27

ø25

ø8.1

ø17

ø14.3

M25�~0.75

D 20X /0.4

WD 3.0��/1.2

Ph1 DL

60.4

1

4.2

636

104.

5

45˚�

45˚�

5

CFI Achromat LWD DL 20x F

24.6

25

60.4

1

4.5

35

35˚�

ø28.2

ø25.5

ø20

ø12.2

ø29

ø32

ø34

M25�~0.75

D 40X /0.55WD 2.1��/0–2

2 1.6 1.2 0.8 0.4 0

CFI Achromat LWD 40x CCFI Achromat LWD DL 40x C

Objectives (magnification) N.A. W.D. (mm) Focal length (mm) Physical depth of focus (µm) Weight (g) Remarks Phase ring

CFI Achromat 4x 0.10 30.0 50.0 27.50 72

CFI Achromat 10x 0.25 6.1 20.0 4.40 85CFI Achromat DL 10x 0.25 6.1 20.0 4.40 85 ph1

CFI Achromat LWD 20x 0.40 3.8 10.0 1.72 90CFI Achromat LWD DL 20x 0.40 3.8 10.0 1.72 90 ph1

CFI Achromat 40x 0.65 0.65 5.0 0.65 130 Spring-loadedCFI Achromat DL 40x 0.65 0.65 5.0 0.65 130 Spring-loaded ph2

CFI Achromat 60x 0.80 0.3 3.3 0.43 130 Spring-loaded

CFI Achromat 100x Oil 1.25 0.18 2.0 0.27 130 Spring-loadedCFI Achromat DL 100x Oil 1.25 0.18 2.0 0.27 130 Spring-loaded ph3

CFI Achromat LWD DL 20x F 0.40 3.0 10.0 1.72 90 Cover glass thickness 1.2mm ph1

CFI Achromat LWD 40x C 0.55 2.7–1.7 5.0 0.91 195 Cover glass correction 0.2mmCFI Achromat LWD DL 40x C 0.55 2.7–1.7 5.0 0.91 195 Cover glass correction 0.2mm ph2

4

60.0

6

4.5

19.9

10

ø25

ø27

M25�~0.75

C1

30˚�

4 X /0.10��/- WD 30

CFI Achromat 4x

60.0

6

4.5

36.5

2.3

510

ø27

ø10

ø16

ø25

M25�~0.75

45˚�

45˚�

10X /0.25��/- WD 6.1

CFI Achromat 10xCFI Achromat DL 10x

60.0

6

ø27

ø25

ø8

ø14.3

ø17

M25�~0.75

45˚�

45˚�

20X /0.40WD 3.8

4.5

364.

16

10

��/0.17

CFI Achromat LWD 20xCFI Achromat LWD DL 20x

ø7

ø13

ø17

ø20

ø25

60.0

6

ø27

M25�~0.75

45˚�

35˚�

34.5

3.7

83

105

40X /0.65WD 0.65��/0.17

CFI Achromat 40xCFI Achromat DL 40x

60.0

6

58

310

34.5

4.1

ø7

ø13

ø17

ø20

ø25

ø27

M25�~0.75

45˚�

35˚�

WD 0.3��/0.17

60X /0.80

CFI Achromat 60x

60.0

6

58

510

34.5

2.2 ø5

ø13

ø16.6

ø20

ø25

ø27

M25�~0.75

45˚�

20˚�

WD 0.18��/0.17

00X / 1.25 oil

CFI Achromat 100x OilCFI Achromat DL 100x Oil

CFI Achromat Series for Brightfield/Phase Contrast (DL)Correction for chromatic aberration in these objectives have been dramatically improved and are at the same levelas the CFI Plan Achromat Series. These objectives were also corrected for spherical aberration and coma andimage flatness across the 22mm field of view has been drastically improved.The result: truly exceptional quality for this class of objectives.The DL phase contrast objectives in this series have more contrast than the DLL objectives for enhanced contrastwith unstained specimens.

CFI60 OBJECTIVES

Page 4: MICROSCOPE COMPONENTS FOR TRANSMITTED …MICROSCOPE COMPONENTS FOR TRANSMITTED LIGHT APPLICATIONS Specifications and equipment are subject to change without any notice or obligation

These objectives provide incredible image flatness over the entire 25mm field of view, with chromatic aberrationcorrected throughout the entire visible spectrum. These objectives are suitable not only for routine laboratory workbut for photomicrography.The DL phase contrast objectives in this series have more contrast than the DLL objectives for enhanced contrastwith unstained specimens.

76

60.0

6

4610

.75

ø31

ø30

M25�~0.75

1X /0.04Plan UW

��/-WD 3.2

CFI Plan Achromat UW 1x

60.0

652.4

5

5

ø20

ø30

M25�~0.75

2X /0.06��/-WD 7.5

Plan UW

CFI Plan Achromat UW 2x

60.0

6

19.9

104.

5ø25

ø27

M25�~0.75

4X /0.��/-WD 3

Plan

CFI Plan Achromat 4x

60.0

6372.

45

10

ø19

ø12

ø25

ø27

M25�~0.75

10X /0.25��/-WD 10.5

Plan

45˚�

45˚�

CFI Plan Achromat 10xCFI Plan Achromat DL 10x

60.0

6

34.5

4.6

4.5

54.

510

ø13ø7

ø17

ø20

ø25

ø27

M25�~0.75

20X /0.40��/ WD 1.3

Plan

45˚�

45˚�

0.17

CFI Plan Achromat 20xCFI Plan Achromat DL 20x

45˚�

30˚�

60.0

6

34.5

7.5

43.

35

10

ø6

ø13

ø17

ø20

ø25

ø27

M25�~0.75

40X /0.65��/ WD0.57

Plan

0.17

CFI Plan Achromat 40xCFI Plan Achromat DL 40x

60

50.5

5.5

3.5

35.

3

ø14

ø7.5

ø18.8

ø30

M25�~0.75

40X /0.65 NWD 0.480

Plan

45˚�

45˚�

��/

CFI Plan Achromat 40x NCG

60.0

6 (N

CG

)

51.2

4.5

4.3

5.3

ø14

ø5.5

ø18.8

ø30

M25�~0.75

50X /0.90 oilWD 0.40

Plan

45˚�

30˚�

��/-

CFI Plan Achromat 50x Oil

ø25ø20

ø13

ø5.3

ø16.8

60.0

644.5

2.2

49

5

ø27

M25�~0.75

45˚�

22.5˚�

100 X /1.25WD 0.17

Plan

��/ 0.17

CFI Plan Achromat 100x OilCFI Plan Achromat DL 100x Oil

6051.2

2.2

6.3

2

5

ø14

ø5.5

ø18.8

ø30

M25�~0.75

100 X /0.90 NWD 0.260

Plan

45˚�

20˚�

��/

CFI Plan Achromat 100x NCG

CFI Plan Achromat Series for Brightfield/Phase Contrast (DL)

Objectives (magnification) N.A. W.D. (mm) Focal length (mm) Physical depth of focus (µm) Weight (g) Remarks Phase ring

CFI Plan Achromat UW 1x 0.04 3.2 200.0 171.88 180

CFI Plan Achromat UW 2x 0.06 7.5 100.0 76.39 110

CFI Plan Achromat 4x 0.10 30.0 50.0 27.50 65

CFI Plan Achromat 10x 0.25 10.5 20.0 4.40 85CFI Plan Achromat DL 10x 0.25 10.5 20.0 4.40 85 ph1

CFI Plan Achromat 20x 0.40 1.3 20.0 1.72 85CFI Plan Achromat DL 20x 0.40 1.3 20.0 1.72 85 ph1

CFI Plan Achromat 40x 0.65 0.57 5.0 0.65 115 Spring-loadedCFI Plan Achromat DL 40x 0.65 0.57 5.0 0.65 115 Spring-loaded ph2

CFI Plan Achromat 40x NCG 0.65 0.48 5.0 0.65 160 Spring-loaded No cover grass

CFI Plan Achromat 50x Oil 0.90 0.4 4.0 0.52 160 Spring-loaded

CFI Plan Achromat 100x Oil 1.25 0.17 3.3 0.52 160 Spring-loadedCFI Plan Achromat DL 100x Oil 1.25 0.17 3.3 0.52 160 Spring-loaded ph3

CFI Plan Achromat 100x NCG 0.90 0.26 2.0 0.18 160 Spring-loaded No cover grass

CFI60 OBJECTIVES

Page 5: MICROSCOPE COMPONENTS FOR TRANSMITTED …MICROSCOPE COMPONENTS FOR TRANSMITTED LIGHT APPLICATIONS Specifications and equipment are subject to change without any notice or obligation

98

Objectives (magnification) N.A. W.D. (mm) Focal length (mm) Physical depth of focus (µm) Weight (g) Remarks Phase ring

CFI Plan Fluor DL 4x 0.13 16.4 50.0 16.27 105 Cover glass thickness 1.2mm phL

CFI Plan Fluor DL 10x 0.30 15.2 20.0 3.06 120 Cover glass thickness 1.2mm ph1

Objectives (magnification) N.A. W.D. (mm) Focal length (mm) Physical depth of focus (µm) Weight (g) Remarks Phase ring

CFI Plan Fluor 4x 0.13 17.1 50.0 16.27 110

CFI Plan Fluor 10x 0.30 16.0 20.0 3.06 120CFI Plan Fluor DLL 10x 0.30 16.0 20.0 3.06 120 ph1

CFI Plan Fluor 20x 0.50 2.1 10.0 1.10 175CFI Plan Fluor DLL 20x 0.50 2.1 10.0 1.10 175 ph1

CFI Plan Fluor 40x 0.75 0.72 5.0 0.49 210 Spring-loadedCFI Plan Fluor DLL 40x 0.75 0.72 5.0 0.49 210 Spring-loaded ph2

CFI Plan Fluor 40x Oil 1.30 0.2 5.0 0.25 230 Spring-loaded Stopper

CFI Plan Fluor 60x C 0.85 0.3 3.3 0.38 265 Spring-loaded Cover glass correction 0.11–0.23mm

CFI Plan Fluor 100x Oil 1.30 0.2 2.0 0.25 250 Spring-loaded StopperCFI Plan Fluor DLL 100x Oil 1.30 0.2 2.0 0.25 250 Spring-loaded Stopper ph3

CFI Plan Fluor 100x Oil with iris diaphragm 0.5–1.3 0.2 2.0 0.25 285 Spring-loaded With iris diaphragm

100X/1.30 oil Plan Fluor

DIC H ��/0.17 WD 0.20

ø30

ø5.8

ø17

ø21

5.3

3.8

51.5

4.5

45˚�20˚�

CFI Plan Fluor 100x OilCFI Plan Fluor DLL 100x Oil

17˚�

ø5.3

ø19.8

C2.5

ø23.6

ø32

100X/0.5-1.3 oil Plan Fluor

��/0.17 WD 0.2

1.3 0.7

ø32

ø33.4

ø30

M25�~0.75

5.3

2.9

6.5

7.56.2

2.6

34

CFI Plan Fluor 100x Oil withiris diaphragm

4X / 0.13 Plan Fluor

PhL DL ��/1.2 WD 16.4

M25�~0.75

ø3060

.41

42.8

55.

3

45˚�

CFI Plan Fluor DL 4x

4X / 0.13

Plan Fluor

�� / - WD 17.1

M25�~0.75

ø30

5.3

42.8

ø20

45˚�

CFI Plan Fluor 4x

10X / 0.30

Plan Fluor

DIC L�@� ��/0.17 WD 16.0

M25�~0.75

ø30

5.3

43.9

ø18

45˚�

CFI Plan Fluor 10xCFI Plan Fluor DLL 10x

20X / 0.50

Plan Fluor

DIC M ��/0.17 WD 2.1

M25�~0.75

ø30

5.3

543.

8

ø17

45˚�

ø12

CFI Plan Fluor 20xCFI Plan Fluor DLL 20x

40X / 0.75

Plan Fluor

DIC M ��/0.17 WD 0.72

M25�~0.75

ø30

5.3

5.5

57.2

2

45˚�45˚�

ø10

CFI Plan Fluor 40xCFI Plan Fluor DLL 40x

45˚�

23˚�

40X / 1.30 oil Plan Fluor

DIC H ��/0.17 WD 0.2

M25�~0.75

ø30

ø33.5

5.3

12

2.7

404

3.7

ø8

ø22

ø25.8

CFI Plan Fluor 40x Oil

60X / 0.85 Plan Fluor

DIC M ��/0.11-0.23 WD 0.30

171411 20 23

M25�~0.75

ø30

ø34.5

ø8.5

ø18

ø22

ø33

5.3

3.91.

7

1110

.832

30˚�30˚�

CFI Plan Fluor 60x C

10X / 0.30

Plan Fluor

Ph1 DL ��/1.2 WD 15.2

M25�~0.75

ø30

60.4

1

1.2

445.

3

45˚�

6

CFI Plan Fluor DL 10x

CFI Plan Fluor Series for Phase Contrast (DL)

CFI60 OBJECTIVESCFI Plan Fluor Series for Brightfield/Phase Contrast (DLL)Featuring an extra-high transmissionrate, especially in the ultravioletwavelength, and flatness of fieldcomparable to the CFI Plan Achromatseries, these objectives are designedfor fluorescence observation andphotomicrography. Because of thisimprovement in quality, theseobjectives can function as multi-purpose objectives for brightfield,fluorescence, polarizing, and DICobservations.The DLL (low contrast) phaseobjectives for this series can also beused as universal objectives forphase contrast, fluorescence, DIC,and brightfield techniques.

These objectives have more contrast than the DLL objectives for enhanced contrast with tissue culturespecimens. These objectives are designed for use on inverted microscopes and therefore their markings areupside-down for easy reading.

Page 6: MICROSCOPE COMPONENTS FOR TRANSMITTED …MICROSCOPE COMPONENTS FOR TRANSMITTED LIGHT APPLICATIONS Specifications and equipment are subject to change without any notice or obligation

ø8

ø33

ø28

1910

.412

.514

5.3

3.7

8˚�

M25�~0.75

20X / 0.75 Mlmm Plan Fluor

�� / - WD 0.35

Oil G W0.17 0

ø36

ø34.5

ø30.6

3˚�

30˚�

40˚�

1110

M25�~0.75

ø30

51.4

60.0

6

WD

=8.

5C

G=

0.17

5.3

ø19

45˚�

2X / 0.1 Plan Apo

�� / - WD 8.5

CFI Plan Apochromat 2x

M25�~0.75

ø30

44.2

60.0

6

WD

=15

.7C

G=

0.17

5.3

ø18

30���

4X / 0.2 Plan Apo

�� / - WD 15.7

CFI Plan Apochromat 4x

M25�~0.75

ø30

55.9

60.0

6

WD

=4.

0C

G=

0.17

5.3

ø15

45˚�

10X / 0.45 Plan Apo

�� /0.17 WD 4.0

DIC L

CFI Plan Apochromat 10x

M25�~0.75

ø30

ø33.5

12

60.0

6

WD

=1.

0C

G=

0.17

5.3

5.9

2.5

38.5

ø13.5

ø22

ø26

45˚�

30˚�

20X / 0.75 Plan Apo

�� /0.17 WD 1.0

DIC M

CFI Plan Apochromat 20xCFI Plan Apochromat DM 20x

3˚�

WD

=0.

14C

G=

0.174.2

5.3

3211

10.8

1.7

171411 20 23

��/0.11-0.23 WD 0.14

40X / 0.95 Plan Apo

DIC M

ø7

ø18

ø22

ø33

M25�~0.75

ø30

ø34.5

60.0

6

30˚�

30˚�

CFI Plan Apochromat 40x CCFI Plan Apochromat DM 40x C

ø25

ø30

ø32

12

60.0

6

WD

=0.

16 (

oil)

CG

=0.

17

54.

74.

538

.5

ø8

ø18.8

ø22.8

45˚�

5˚�

40X / 1.0 oil Plan Apo

�� /0.17 WD 0.16

DIC H

30˚�

CFI Plan Apochromat 40x OilCFI Plan Apochromat DM 40x OilCFI Plan Fluor 20x MI

Objective N.A. W.D. Focal length Physical depth Weight Remarks(magnification) (mm) (mm) of focus (µm) (g)

CFI Plan Fluor 20x MI 0.75 0.35–0.33 10.0 0.49 300 Multi-immersionOil-glycerine-water

Objectives (magnification) N.A. W.D. (mm) Focal length (mm) Physical depth of focus (µm) Weight (g) Remarks Phase ringCFI Plan Fluor ELWD 20x C 0.45 8.1–7.0 10.0 1.36 195 Cover glass correction 0–2mmCFI Plan Fluor ELWD DM 20x C 0.45 8.1–7.0 10.0 1.36 195 Cover glass correction 0–2mm ph1CFI Plan Fluor ELWD 40x C 0.60 3.7–2.7 5.0 0.76 195 Cover glass correction 0–2mmCFI Plan Fluor ELWD DM 40x C 0.60 3.7–2.7 5.0 0.76 195 Cover glass correction 0–2mm ph2CFI Plan Fluor ELWD 60x C 0.70 2.1–1.5 3.3 0.56 210 Cover glass correction 0.5–1.5mmCFI Plan Fluor ELWD DLL 60x C 0.70 2.1–1.5 3.3 0.56 210 Cover glass correction 0.5–1.5mm ph2

ø25.5

ø28.7

ø32.5

ø16.4

ø29

ø32

ø34

60.4

11.

2

22.2

1.8

1.8

11.5

135

5˚�

7˚�

25˚�

LWD 20X/0.45 Plan Fluor

��/ 0-2 WD 7.4

2 1.6 1.2 0.8 0.4 0

Sta

ndar

d�th

ickn

ess

CFI Plan Fluor ELWD 20x CCFI Plan Fluor ELWD DM 20x C

ø27.5

ø29

ø32.5

ø13.3

ø29

ø32

ø34

60.4

1

26.5

2.5

2.5

11.5

135

5���

7˚�

20˚�

LWD 40X/0.60 Plan Fluor

��/0-2 WD 3.7 - 2.7

2 1.6 1.2 0.8 0.4 0

M25�~0.75

CFI Plan Fluor ELWD 40x CCFI Plan Fluor ELWD DM 40x C

ø26.3

ø32.5

ø10

ø29

ø33

ø34

60.4

1

3311

.513

5

6

7.5˚�

9˚�

22

.5���

WD 60X/0.70 Plan Fluor

0.5-1.5 WD 2.1-1.5

1.5 1.2 0.81 0.5

M25�~0.75

CFI Plan Fluor ELWD 60x CCFI Plan Fluor ELWD DLL 60x C

CFI Plan Fluor ELWD Series for Brightfield/Phase Contrast (DM/DLL)

Multi-Immersion Objective for Brightfield

CFI Plan Apochromat Series for Brightfield/Phase Contrast (DM)Offering superb flatness of field, high UV transmission rates, and high Numerical Apertures with extra long working distances, these objectives arewell suited for fluorescence observations, especially with inverted microscopes. Because of their superior optical design, CFI Plan Fluor ELWD DMobjectives can be used universally for all other observation techniques, including brightfield, fluorescence, phase contrast, and Nomarski DIC.The DM phase contrast objectives have higher contrast to enhance the image of very thin, highly detailed specimens with low phase gradients.The DLL objectives have less contrast for thicker, more contrasty specimens.

These objectives feature longer working distances with high Numerical Apertures and is designed to correct alloptical aberrations throughout the visible spectrum from violet to red from center to edges across the entire 25mmfield of view. Superior image flatness and color reproduction, plus resolving power at the theoretical limit of today'soptical technology are also featured.The DM phase contrast Plan Apochromats have higher contrast to enhance images of very thin, highly detailedspecimens with low phase gradients.

This multi-purpose immersion objective has a higher numerical aperture andsuperior contrast. The use of immersion media helps eliminate surfacereflections and provides an image with higher contrast. It is an excellentobjective for fluorescence, brightfield, and DIC observation techniques.This unique objective has a correction collar that allows it to be used withimmersion oil, glycerin, and water (with and without a cover glass).

CFI60 OBJECTIVES

Page 7: MICROSCOPE COMPONENTS FOR TRANSMITTED …MICROSCOPE COMPONENTS FOR TRANSMITTED LIGHT APPLICATIONS Specifications and equipment are subject to change without any notice or obligation

1312

Objectives (magnification) N.A. W.D. (mm) Focal length (mm) Physical depth of focus (µm) Weight (g) Remarks

CFI S Fluor 10x 0.50 1.2 10.0 1.10 275 Spring-loaded

CFI S Fluor 20x 0.75 1.0 10.0 0.49 275 Spring-loaded

CFI S Fluor 40x C 0.90 0.3 5.0 0.34 285 Spring-loaded Cover glass correction 0.11–0.23mm

CFI S Fluor 40x Oil 1.30 0.22 5.0 0.25 275 Spring-loaded

CFI S Fluor 100x Oil 1.30 0.2 2.0 0.25 285 Spring-loaded

5.3

8.2

1.2

1238

.564

ø32

ø8

ø19

ø24

ø30

M25�~0.75

10X / 0.50��/ WD 0.120.17

S Fluor

CFI S Fluor 10x

1

5.3

5.9

1241

64.2

ø9

ø22ø25

ø33.5

ø30

M25�~0.75

20X / 0.75��/ WD 1.00.17

S Fluor

CFI S Fluor 20x

527

8.5

3.1

10.5

10.5

ø7.5

ø18

ø26

ø33

ø34.5

ø33

ø30

M25�~0.75

11 14 17 20 23

40X / 0.9/ WD 0.30.11-0.23

Fluor

CFI S Fluor 40x C

512

376.

54.

2

ø32

ø30

ø8.3

ø20

ø23.8

M25�~0.75

40X / 1.30��/ WD 0.220.17

S Fluor

Oil

CFI S Fluor 40x Oil

532

7.5

7.5

9.8

2.9

64.7

ø32

ø30

ø6

ø17

ø24

ø29.5

M25�~0.75

1.3 0.7

100 X /0.5-1.3 oilS Fluor

��/ WD 0.20.17

CFI S Fluor 100x Oil

30˚�

30˚�

60.0

6

3211

10.8

4.21.

7

5.3

ø18

ø7

ø22

ø33

ø34.5

ø30

M25�~0.75

WD

=0.

15C

G=

0.17

3˚�

��/ WD 0.150.11-0.23

11 14 17 20 23

60X /0.95Plan Apo

DIC M

CFI Plan Apochromat 60x CCFI Plan Apochromat DM 60x C

60.0

6

404

3.7

125.

3

ø7.2

ø22

ø25.8

ø33.5ø30

M25�~0.75

CG

=0.

17W

D=

0.21

(oi

l)

8˚�

45˚�

22.5˚�

��/ WD 0.210.17

60X /1.40 oilPlan Apo

DIC H

CFI Plan Apochromat 60x OilCFI Plan Apochromat DM 60x Oil

7˚�

5˚�

30˚�

30˚�

59.7

5.3

23.5

1111

.910

.42.

9

ø29

ø7.5

ø33.4

ø36.5

ø35.2

ø30

M25�~0.75

15 16 17 18

60X /1.20 WIPlan Apo

��/ WD 0.220.15-0.18

CFI Plan Apochromat 60x WI

20˚�

59.8

60.0

6

5.3

51.5

4.5

3.8

ø30

ø5.8

ø17

ø21

M25�~0.75

C2

WD

=0.

13

��/ WD 0.130.17

100 X /1.40 oil

Plan Apo

DIC H

CFI Plan Apochromat 100x OilCFI Plan Apochromat DM 100x Oil

20˚�

60

59.8

5.3

51.5

4.5

3.8

ø5.8

ø17

ø21

ø30

M25�~0.75

C2

WD

=0.

17

��/ WD 0.170

00X /1.40 oilN

Plan Apo

CFI Plan Apochromat 100x NCG Oil

CFI Plan Apochromat Series for Brightfield/Phase Contrast (DM)

Objectives (magnification) N.A. W.D. (mm) Focal length (mm) Physical depth of focus (µm) Weight (g) Remarks Phase ring

CFI Plan Apochromat 2x 0.10 8.5 100.0 27.50 180

CFI Plan Apochromat 4x 0.20 15.7 50.0 6.88 180

CFI Plan Apochromat 10x 0.45 4.0 20.0 1.36 220

CFI Plan Apochromat 20x 0.75 1.0 10.0 0.49 240 Spring-loadedCFI Plan Apochromat DM 20x 0.75 1.0 10.0 0.49 240 Spring-loaded ph2

CFI Plan Apochromat 40x C 0.95 0.14 5.0 0.30 270 Spring-loaded Cover glass correction 0.11–0.23mmCFI Plan Apochromat DM 40x C 0.95 0.14 5.0 0.30 270 Spring-loaded Cover glass correction 0.11–0.23mm ph2

CFI Plan Apochromat 40x Oil 1.0 0.16 5.0 0.42 240 Spring-loaded StopperCFI Plan Apochromat DM 40x Oil 1.0 0.16 5.0 0.42 240 Spring-loaded Stopper ph3

CFI Plan Apochromat 60x C 0.95 0.15 3.3 0.30 285 Spring-loaded Cover glass correction 0.11–0.23mmCFI Plan Apochromat DM 60x C 0.95 0.15 3.3 0.30 285 Spring-loaded Cover glass correction 0.11–0.23mm ph2

CFI Plan Apochromat 60x Oil 1.40 0.21 3.3 0.21 285 Spring-loaded StopperCFI Plan Apochromat DM 60x Oil 1.40 0.21 3.3 0.21 285 Spring-loaded Stopper ph3

CFI Plan Apochromat 60x WI 1.20 0.22 3.3 0.25 285 Spring-loaded Cover glass correction 0.15–0.18mmWater immersion

CFI Plan Apochromat 100x Oil 1.40 0.13 2.0 0.21 245 Spring-loaded StopperCFI Plan Apochromat DM 100x Oil 1.40 0.13 2.0 0.21 245 Spring-loaded Stopper ph3

CFI Plan Apochromat 100x NCG Oil 1.40 0.17 2.0 0.21 245 Spring-loaded Stopper No cover glass

CFI S Fluor SeriesThese objectives ensure a high transmission rate of ultraviolet wavelengths down to 340 nm for fluorochromes likeindo-1, fura-2, and fluor-3. Also, these objectives have improved signal-to-noise ratios (S/N) for short wavelengths andhave high N.A., making the fluorescence images they produce significantly sharper and brighter.

CFI60 OBJECTIVES

Page 8: MICROSCOPE COMPONENTS FOR TRANSMITTED …MICROSCOPE COMPONENTS FOR TRANSMITTED LIGHT APPLICATIONS Specifications and equipment are subject to change without any notice or obligation

100X / 1.30 oil

Plan Fluor

DIC H ��/ 0.17 WD 0.20

28

22.5

5

ø36f7 -0.025�-0.050

ø33

App

rox.

70~

170

148

M38�~0.5

Image plane

Objective's shoulder

2nd Objective Lens Unit

2nd Objective Lens Unit

15

This unit is attached to infinity objectives when neither abinocular or trinocular eyepiece tube is used.• Infinity Tube Lens Unit for CFI objectives (chromatic aberration-

free infinity corrected objectives).• Focusing distance: 200mm (7.87 in.)• To obtain the optimal objective performance, keep the distance

between the tube lens and the objective’s shoulder within100–200 mm (3.84–7.87 in.).

These objectives (no cover glass) are designed with a narrow top and a 2mm working distance to allowmicroelectrodes to be inserted into the specimen at 45°. They use ceramic and glass nose cones to isolate themthermally and electronically. Since they are FLUOR lenses, they are designed for fluorescence and have very hightransmission in the UV and IR wavelengths with a high signal-to-noise ratio for superior images.

These objectives have been completely redesigned by Nikon, allowing the contrast direction to be changed usinga modulator inside the objective. That direction, once set, is maintained over the entire magnification range from10X to 40X.Note: Hoffman Modulation Contrast and HMC are registered trademarks of Modulation Optics Inc.

14

45˚�

45˚�

10X / 0.25

MC1 HMC

�� / 1.2 WD 5.2

ø25

60.4

1

4.5

1310

2.5

28.5

ø13.3

ø10

M25�~0.75

ø27

ø27.8

4.5

CFI HMC 10x

45˚�

45˚�

D 20X / 0.4

MC2 HMC

�� / 1.2 WD 3.0

ø25

60.4

1

4.8

1310

28.5

ø16.5

ø8

ø15

M25�~0.75

ø27

ø27.8

4.5

CFI HMC 20x F

35˚�

ø28.8ø25.5ø20ø12

ø32ø33.3

WD 40X / 0.55

MC3 HMC

��/0- 2 WD 2.7-1.7

00.40.81.21.62

60.4

1

1

5.6

1911

.58

135

M25�~0.75

ø34

ø29

ø32

CFI HMC LWD 40x CObjectives (magnification) N.A. W.D. (mm) Focal length (mm) Physical depth of focus (µm) Weight (g) Remarks

CFI HMC 10x 0.25 5.2 20.0 4.40 130 Cover glass thickness 1.2mm

CFI HMC 20x F 0.40 3.0 10.0 1.72 130 Cover glass thickness 1.2mm

CFI HMC LWD 40x C 0.55 2.7–1.7 5.0 0.91 210 Cover glass correction0–2mm

Objectives (magnification) N.A. W.D. (mm) Focal length (mm) Physical depth of focus (µm) Weight (g) Remarks

CFI Fluor 10x W 0.30 2.0 20.0 4.06 210 Water dipping

CFI Fluor 40x W 0.80 2.0 5.0 0.57 210 Water dipping

CFI Fluor 60x W 1.0 2.0 3.3 0.37 210 Water dipping

10X / 0.30 WDIC L

Fluor

�� / 0 WD 2.0

M25�~0.75

ø29

ø20.5

ø24

ø26

ø3.5

ø5

58.5

60.5

2 (W

ater

dip

ping

)

32.7

121.

58.

53.

8

5

48˚� 45˚�55˚�

CFI Fluor 10x W

40X / 0.80 WDIC M

Fluor

�� / 0 WD 2.0

M25�~0.75

ø29

ø19

ø23

ø25.5

ø4

ø11.5

58.5

60.5

32.7

4.5

11.3

55

5

45˚� 45˚� 50˚�

2 (W

ater

dip

ping

)

CFI Fluor 40x W

60X / 1.00 WDIC H

Fluor

�� / 0 WD 2.0

M25�~0.75

ø29

ø19.2

ø5.5

ø23

ø25.5

58.5

60.5

32.7

111

.35.

58

5

33.7˚� 30˚

40˚

2 (W

ater

dip

ping

)

CFI Fluor 60x W

Water Dipping Series CFI HMC® Series for Hoffmann Modulation Contrast®

CFI60 OBJECTIVES

Page 9: MICROSCOPE COMPONENTS FOR TRANSMITTED …MICROSCOPE COMPONENTS FOR TRANSMITTED LIGHT APPLICATIONS Specifications and equipment are subject to change without any notice or obligation

Markings on CFI60 Objectives

16 17

Condensers

Type N.A. Object distance (mm) Magnification

C-C Abbe Condenser, Dry Type 0.9 1.9 4–100x

C-C Achromat Condenser 0.8 4.2 4–100x

Achromat Swing-out Condenser 0.9/0.22 1.8 2–100x

C-C Achromat /Aplanat Condenser 1.40 1.6 10–100x

LWD Achromat Condenser 0.65 10.2 4–40x

C-C Low Power Condenser 0.13 10.2 1–4x

Dark Field Condenser, Oil 1.2–1.43 1.5 20–100x

Dark Field Condenser, Dry 0.8–0.95 4.0 20–40x

ø55

ø59

ø43

ø40

ø54

R40

0.7

854

.9

55.6

C-C Abbe Condenser, Dry Type

ø55

ø36.8

ø58R40

2.0

854

.5

56.5

ø40

ø54

C-C Achromat Condenser

ø27 ø50

ø40

R40

ø10

9

34.5

55

7.5

8

1913

11.5

ø60

ø8

582.5 21

14.5 6

Achr 0.90 JAPAN

4.5˚�

30˚�

70˚�

0.8 0.6 0.4 0.2

4.5˚�

Achromat Swing-out Condenser

ø54

ø40

ø62

55

54.4

0�

-0.2

70˚�

1.0

0.6

(Oil)

10(S

lidgl

ass

thic

knes

s)

1.4 1.2 1.0 0.8 0.6 0.4 0.2Achr-ADl N.A=1.4 JAPAN

C-C Achromat /Aplanat Condenser

ø59

ø42

ø22

ø36.8

ø54.5

1.5

8.6

8

63.4

54.8

29.8

24.5

12

ø40

LWD Achromat Condenser

R40 ø58

ø55

ø54

ø38

58.7

88.

3

67

ø40

C-C Low Power Condenser

ø39

ø40

ø56

ø34.5

55.5

550.

5(O

il)20

70˚�

149.

5

81

Dark Field Condenser�Oil 1.43-1.20�

JAPAN

(Slid

glas

s th

ickn

ess)

Dark Field Condenser, Oil

ø35.5

ø40

ø56

55.6

54

70˚�

20.6

18

Dark Field Condenser�Dry 0.95-0.80�

JAPAN

(Slid

glas

s th

ickn

ess)

Dark Field Condenser, Dry

ø30

11.5

14.9

C-CEL

C-CEL Expander Lens (for darkfield)

(1) Magnification and Color CodeA color coded ring on the barrel identifiesthe magnification of the objective:

(2) Numerical Aperture (N.A.)N.A. is the most important factor in definingthe performance characteristics of an objective.N. A.= n sin θn: the refractive index of the media at d-line (587nm)For dry objective n=1.000 (air)For oil objective n=1.515 (oil)For water objective n= 1.333 (water)θ:Half angle of incident rays to the top lens of the objective

The higher N.A., the higher resolving power.When the resolving power is defined as the power torecognize the two points,

R= λ2N.A.

or

R= λN.A. Obj. +N.A. Cond.

The higher N.A. the brighter image we take.

Brightness: B ∝ N.A. 2

Total Magnification

The higher N.A., the shallower the depth of focus (DOF).

DOF = λN.A. 2

(3) Working DistanceWorking distance (W.D.) defines the distance betweenthe top lens of the objective and the surface of thecover glass.CFI60 objectives can offer longer working distance withhigh numerical aperture.

(4) Correction RingDry objectives with high Numerical Aperture aresusceptible to spherical and other aberrations whichcan impair resolution and contrast when used with acover glass whose thickness differs from the specifiedvalue. A 1 1/2 cover glass (0.17mm thick) should be usedas standard, however not all 11/2 cover glasses areexactly 0.17mm and many specimens have mediabetween them and the cover glass. The correction ringis used to adjust for these subtle differences to ensurethe optimum objective performance.

How to use the correction ring● Position the ring at 0.17. The thickness of thestandard cover glass is 0.17mm.● Focus the lens on a small artifact in the specimen.●Rotate the ring very slightly and focus the lens againto check if the image has improved or degraded.●Repeat the above step to determine if the image isimproving or degrading in the direction you areturning the ring.● If the image has degraded, follow the same

procedure in the opposite direction to find the position offering optimum resolving power and contrast.

(5) Cover GlassFor optimum performance, the thickness of the coverglass should be 0.17mm. For example, at N.A.=0.95, a0.01mm difference in thickness reduces imageformation by 45% from the ideal image.

(6) Application MarkingsDIC: for Differential Interference ContrastDM: Phase contrast, Dark contrast middle typeDL: Phase contrast, Dark contrast light typeDLL: Phase contrast, Lower contrast type

(7) Immersion Oil—cleaningAfter using immersion oil, gently blot the lens dry withlens tissue. Then slightly moisten a piece of lens tissuewith petroleum benzene (Naphtha) and clean off alltraces of the oil from the immersion objective. Cleaningis essential for water immersion objectives as well; afteruse, wipe the water off the top lens.

LightBlue

LightBlue

CobaltBlue WhiteBlackColor code Gray Red Yellow Green

1XMag. 2X 4X 10X 20X 40X 50X 60X 100X

0.3

N.A.Difference in cover glass thickness

0.01mm 0.02mm

0.450.70.850.95

100%100988145

100%100924329

(1) Color Code

(1) Magnification (2) Numerical Aperture

(3) Working Distance

(4) Correction Ring

(5) Cover Glass

(6) Application Markings

(7) Immersion Oil—cleaning

Oil Dry

n = 1.515 θn = 1.000

Slide Glass

Cover Glass

Objective lens

{ }

Nikon offers a wide variety of CFI objectives. To assist the user they are clearly marked with informationon the objective barrel such as: which DIC module or Phase Ring to use.

CFI60 OBJECTIVES

Page 10: MICROSCOPE COMPONENTS FOR TRANSMITTED …MICROSCOPE COMPONENTS FOR TRANSMITTED LIGHT APPLICATIONS Specifications and equipment are subject to change without any notice or obligation

67.8306.6

10.3 0.6

7

37.8

24 5

20.5

41.5

(Center of lamp)

(Cen

ter

of p

in)

(Cen

ter

of fi

lam

ent)

�i35.5�‘39.5�j�

ø57

ø50

Eyepiece Tubes

1918

50

76

30 84.6

4

12 24.5 13.5

90˚�

ø71

ø51

ø67

(Dat

um c

lam

p fa

ce)

(Dat

um c

lam

p fa

ce)

C-LA HMX Lamphouse Adapter

3.5

15

20.5

7628.5

ø71

M50�~

0.75

(Dat

um c

lam

p fa

ce)

(Dat

um c

lam

p fa

ce)

HMX Connecting Adapter SB-X1(Bayonet to Screw)

3

7

4

M50�~0.75-6HSectional View

ø55

ø61

HMX Connecting Adapter SB-X2(Screw to Bayonet)

Y-TB Binocular Tube F.O.V. 22mm

Y-TE Ergonomic Binocular Tube F.O.V. 22mm with tilting angle of 8° to 32° and telescopic mechanism of ±15mm movement

Y-TF Trinocular Tube “F” UW Two-way: 100 : 0 or 0 : 100 (observation : photo) F.O.V. 22mm or 25mm with eyepiece reticule orientation adjustment mechanism

Y-TT Trinocular Tube “T” UW Three-way: 100 : 0, 20 : 80, or 0 : 100 (observation : photo) F.O.V. 22mm or 25mm with eyepiece reticule orientation adjustment mechanism

60

100

80 64

70

Y-TB Binocular Tube

110

100

9864�

(Tilt

ing

sect

ion)

105

(Min

. dis

tanc

e)

Approx. 11190.5

863

R32

R40

508˚

(M

in. a

ngle

)

50.5 77

ø51.3

705

Y-TE Ergonomic Binocular Tube

76.5

151.

566

.5

218.

5

120

16.5

50

ø52

Y-TF Trinocular Tube “F” UW

135.9

102.5

27.1

ø58

58.5

ø52

ø59

50

172

73.710

0

16.5

3

�i239�j�

Y-TT Trinocular Tube “T” UW

Illumination Systems

105

2461 (Center of lamp)

139

80.5

171

801

170

Lamphouse HMX-3B

2461 (Center of lamp)

105

80.5

168.5

139

171

117

080

Lamphouse HMX-4B, built-in back mirror

137.

367

.8

109

7769

.5

ø42

4

ø56

4768

26

10

2

136

34

6

2

20

80

102

148

Lamphouse HMX

99.581.5

70 8.5

52.5

51

76.5

138.

5

40

Mercury Lamp Socket S 100W for HMX-3B

Halogen Lamp Socket 100W/HMX38.5

7.5

12

ø45

ø33

ø31.1 223

6

Center �of �lamp

C-FC Epi-Fl Collector Lens

36 11

3

3 21

ø45

ø36

Center �of �lamp

36.5

3 22

12.5

ø45 Center of

lamp

Quartz Epi-Fl Collector Lens Collector Lens/QH100W

Page 11: MICROSCOPE COMPONENTS FOR TRANSMITTED …MICROSCOPE COMPONENTS FOR TRANSMITTED LIGHT APPLICATIONS Specifications and equipment are subject to change without any notice or obligation

Nosepieces

21

Intermediate Tubes

100

353.5

322

126.580

264

248

120.

655 34

.520

.5

80

Y-FL Epi-Fl Attachment

100

13.5

4525

5

50.5

57.522

175

ø52

ø51

285.5(Top View)

(Right-side View)

5545

1000

Y-IDP Double Port

20

Eyepieces

Type (magnification) F.O.V. Remarks

CFI 10x with diopter adjustment 22mm

CFI 10x M Photomask with diopter adjustment 22mm With photomask reticule

CFI 12.5x with diopter adjustment 16mm

CFI 15x with diopter adjustment 14.5mm

CF

I 10X

/ 22

ø34

ø38

.8

ø30

22.5

48–56

CFI 10x with diopter adjustment

CF

I 10X

/ 22M

ø34

ø38

.8

ø30

22.5

48–56

CFI 10x M Photomask with diopter adjustment

CF

I 12.5X

/ 16

22.5

42–47

ø30

ø35

ø38

.8

CFI 12.5x with diopter adjustment

22.5

37–41

ø30

ø35

ø38

.8

CF

I 15X

/ 14.5

CFI 15x with diopter adjustment

22.5

V-N

JAP

AN

123.2

ø121

50

53 48 2

1

6 2

3

V-NA Motorized Sextuple Nosepiece

22.5

V-N

D JAP

AN

123.2

ø121

50

53 48

V-ND MG-DIC Intelligent Sextuple Nosepiece

22.5

50

JAP

AN

105.8

ø106

53 51.1 48

C-N Sextuple Nosepiece

22.5

C-N

D D

IC6

123.2

ø121

50

53 48

C-ND Sextuple DIC Nosepiece

Page 12: MICROSCOPE COMPONENTS FOR TRANSMITTED …MICROSCOPE COMPONENTS FOR TRANSMITTED LIGHT APPLICATIONS Specifications and equipment are subject to change without any notice or obligation

23

ø23.1

ø53

ø42

Focal point

48

122.

8

27.0

30.2

3.3

ø23.1

ø53

ø42

Focal point

38

111.

2

2730

.24

ENG-Mount TV Adapter 0.45x(for Trinocular Tube “F”, “T”)

CCTV Camera Adapters

22

3 9.3

ø12

7227

ø52

ø38

35

ø52

V-T Photo Adapter

76.5

ø51

ø38

ø38

ø59

Y-T TV Tube for C-Mount Adapter A

ø50

ø25.4

ø38

41

25.5

11.4

717

.526

C-mount screw

TV camera imaging plane

30˚�

C-Mount TV Adapter A

ø34

Image plane �on eyepiece tube side

31

10

4475

ø30

Relay Lens 1x I

ø31

ø42

ø46

7.5

64.5

27.3

91.8

17.5

26

95.8

45˚�

C-Mount TV Adapter61

.3�}0.

148

64.3

53.3

27.8

11.5

ø42

ø63

ø62ø47

ENG-Mount TV Adapter

JAPAN

ø42

ø54ø58.5

ø23.1

67.3

130

40.2

Zoom Lens

ø44

54.3

7.5

42.8

17.5

26

11.5

4

ø54

45˚�

C-mount screw

C-Mount Zooming Adapter

4812

.37.

5

22.8

ø44ø57

ENG-Mount Zooming Adapter

ø23.1

Focal point

30.7

100.

1

27.3

417.5

26

ø53

ø42

C-Mount TV Adapter 0.45x

ø23.1

Focal point

30.7

116.

8

27417

.526

ø53

ø42

C-Mount TV Adapter 0.6x

ø38

68

4229

ø53

Focal point

C-Mount TV Adapter VM 2.5xø38

109

83

ø53

29

Focal point

C-Mount TV Adapter VM 4x

ENG-Mount TV Adapter 0.6x(for Trinocular Tube “F”, “T”)