Measuring pH Using the Orion 3 Star Plus pH Meter with Printer 1

Measuring pH Using the Orion 3 Star Plus pH Meter with Printer

Measuring pH Using the Orion 3 Star Plus pH Meter with Printer 2

Orion 3 Star Plus pH Meter

Front of Meter & Keypad

Measuring pH Using the Orion 3 Star Plus pH Meter with Printer 3

Start Up• Turn Printer ON• Press POWER Key on

Meter Display will read:

Star PLUS Software Version and

current method

Header information will print:

Meter Serial Number Meter Software Version Method currently set

• Check print quality

Measuring pH Using the Orion 3 Star Plus pH Meter with Printer 4

Calibrate Method 1

Select Method (# decimals) to be used for samples

Press MEASURE to set method

Open Fill Hole

Rinse electrode and blot carefully

Measuring pH Using the Orion 3 Star Plus pH Meter with Printer 5

Calibrate Method 1

Press CALIBRATE

CAL 1 Displayed..

Immerse electrode in first buffer

• pH will stop flashing when reading stable

• Buffer value will be displayed with leading zeroes

Measuring pH Using the Orion 3 Star Plus pH Meter with Printer 6

Calibrate Method 1After pressing CALIBRATE, • CAL 2 displayed• pH flashes while reading in progress

• Remove electrode from first buffer, rinse & blot

• Immerse electrode in second buffer

• pH will stop flashing when reading stable

• Buffer value will be displayed with leading zeroes

Measuring pH Using the Orion 3 Star Plus pH Meter with Printer 7

Calibrate Method 1

After pressing CALIBRATE, • CAL 3 displayed• pH flashes while reading in progress

• Remove electrode from second buffer, rinse & blot

• Immerse electrode in third buffer

• pH will stop flashing when reading stable

• Buffer value will be displayed with leading zeroes

Measuring pH Using the Orion 3 Star Plus pH Meter with Printer 8

Completing CalibrationPress MEASURE to print & save calibration results

• Check date and time (not visible in photo)

• Check mV for each buffer

• Check slope 1 and slope 2

• Initial & date printout

Measuring pH Using the Orion 3 Star Plus pH Meter with Printer 9

Verifying Instrument Performance• Remove electrode from buffer, rinse & blot

• Immerse electrode in QC (6.86) bufferPress MEASURE

pH will stop flashing when reading stable

Press MEASURE to print result

• Initial & date• Identify sample as QC Buffer

Measuring pH Using the Orion 3 Star Plus pH Meter with Printer 10

Documenting Calibration

• Check Dates & Times• Check Method• Check Calibration #• Check Limits:

• 4.01 +177 mV ± 30 mV

• 7.00 0 mV ± 30 mV

• 10.01 -177 mV ± 30 mV• Slope 1 and 2:

95% - 102%• QC Buffer: 6.86 ± 0.05 Method 1: 6.8 – 6.9 Method 2: 6.81 – 6.91

Calibration & Initial QC Buffer on form

Measuring pH Using the Orion 3 Star Plus pH Meter with Printer 11

Measuring Sample pH• Remove electrode from buffer or storage solution, rinse & blot

• Immerse electrode in samplePress MEASURE

pH will stop flashing when reading stable

Press MEASURE again to print result

• Initial & date• Identify sample

Measuring pH Using the Orion 3 Star Plus pH Meter with Printer 12

Reviewing Results• Check Dates & Times

• If > 2 hours since last QC Buffer run, instrument performance must be verified by running the QC Buffer as a Batch control

• Check Method• 1 decimal for any product QC and most

reagents/solution• 2 decimals if reagent procedure

requires more sensitivity• NO ROUNDING

• Check Calibration #• Match to the most recent calibration for

the method selected

• Attach product results to Product QC Forms

• Attach reagent/solution pH measurements to Reagent pH Log [19.4.frm024]