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Pan Pacific Pork Expo 2006. Pig Reproduction. M.E. Wilson. 30 Pigs weaned/ sow/ year. PMWS. Economics of reproduction. 3-3.5 US cents per market pig/EBV point above the average $2.35 improvement in each generation. Natural Mating. Natural Service vs Artificial Insemination. - PowerPoint PPT Presentation
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M.E. Wilson
Pig Reproduction
Pan Pacific Pork Expo 2006
30 Pigs weaned/ sow/ year
Economics of reproduction
3-3.5 US cents per market pig/EBV point above the average
$2.35 improvement in each generation
5
Natural MatingNatural Service vs Artificial Insemination
Natural
Service
Artificial
Insemination
Total sperm
cells
30-80 billion 3 billion
Total volume 200 – 400 ml 75-85 ml
Seminal
Plasma/
insemination
160-350 ml 8-12 ml
Boar ID Volume % viable cells Mean recovery
7 307 75.3 52%
12 286 70.6 24 %
14 324 66.6 35%
16 199 47 20%
17 234 83.6 39%
Lebowa and Wilson, unpublished data, 2005
Which Boar will have the Highest Fertility?
Mean Fertility Results
Boar ID services Farrowing % Total born
7 30 93 13.18
12 29 83 12.74
14 31 45 9.22
16 27 100 12.04
17 27 52 6.5
Lebowa and Wilson, unpublished data, 2005
Single Boar 93 79.6 9.26Two different Boars 106 89.6 10.68
Number ofMatings
%conception
Litter Size
Thacker and Kirkwood, 1988
Effects of heterospermic matings verseshomospermic matings on litter size
Comparison of Breeding MethodsComparison of Breeding Methods
United Feeds, 1998
MethodNumber of
LittersFarrowing
rate %Total born - mummy
AI- 2 different boars
220 88 12.36
Combo - 1 natural 1AI
218 88 12.06
Natural serv 189 88 11.9
AI- 1boar 185 88 11.62
A
A
A
B
Different superscripts P<.05
B
B
UltrasonographyMonitoring Ovarian Activity
No Follicles
Follicles
0 10 20 30 40 50 60 70
6
5
4
3
WE
I (d
)
Estrus (h)
OEOI ED
Kemp and Soede, 1996
follicles
Interval between insemination and ovulation
Sows with >90% Normal Embryos
Fertilization %
Insemination 48-40 17 29
Pre-ovulation 40-32 14 37
32-24 47 47
24-16 79 79
16-8 83 94
8-0 86 93
Ovulation 0-8 54 75
Post-ovulation 8-16 53 62
The effects of the interval between insemination and ovulation onFertilization rate and embryo viability (Soede, et.al., 1995a)
Synchronized Estrus and ovulation
500 – 750 IU of eCG Pregnecol (Canada) 400 IU eCG and 200 IU hCG (PG 600)
42 hours after hCG ovulation typically occurs 36-38 hours after pLH ovulation will be induced
Billions sperm cells/dose
Number of doses
3 30
2.5 36
2 45
1.5 60
IUI - .6 150
Managing dose potential per boar
The issue is not TECHNOLOGY
The issue is The issue is IMPLEMENTATIONIMPLEMENTATION
Transcervical
Deep Uterine
Artificial Insemination
Traditional
Treatment N Total Pigs
+/- Control
Conventional 4
billion
100 947 -
IUI 4 billion 100 1086 + 139
IUI 1 billion 100 892 - 55
IUI .5 billion 100 712 - 235
The number of pigs per 100 matings with 0.5, 1or 4 billion cells transcervical1 or 4 billion cells
intra cervical
1 Transcervical catheter passage was 94%. Hemorrhaging observed on Catheter 4% of matings (n=620) K. Rozeboom
Sexed Sperm Cells Non-surgical implantation of embryos Frozen Semen Expanding genetic potential and reducing
variation
Let’s jump into the future
Sperm sexing technology
• Based on the fact that more (2.8% – 7%) DNA is contained in X-bearing than in Y-bearing sperm
Surgically Flushing Embryos
Midwest Commercial ET Project
Embryo Survival to Birth from Farrowed Sows
Piglet Genotype
Total BornEmbryos
Transferred (farrowed sows only)
Pigs per Embryo
Duroc 182 375 .49
White 66 103 .64
Total 248 478 .52
Litter of 14 pigs from non-surgical transfer
15 embryos transferred 14 pigs born alive
CloningDolly, the sheep (1996)
First mammal to be cloned from adult cells (mammary)
Dolly
Surrogate mother
Viable cloned offspring (litter size ave. = 4-8)
Adult donor
Cloned embryosTransferred within24 hrs - Porcine
Surgical transfer into oviduct- 50-100 clones
Chromatin Transfer
Benefits of Cloning Advance superior genetics
Feed efficiency Rate of gain Carcass traits
Gene markers for disease Research model
Removes more variation Medical models
Testing human diseases