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Effect of atmospheric deposition of nitrogen on the fixation and assimilation of nitrogen by wild lupine (Lupinus perennis) in the sand dunes of Lake Michigan Lauren Avans Purdue University Calumet, Hammond, IN Faculty Advisor: Young D. Choi

Lauren Avans Purdue University Calumet, Hammond, IN Faculty Advisor: Young D. Choi

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Effect of atmospheric deposition of nitrogen on the fixation and assimilation of nitrogen by wild lupine ( Lupinus perennis ) in the sand dunes of Lake Michigan. Lauren Avans Purdue University Calumet, Hammond, IN Faculty Advisor: Young D. Choi . Introduction. - PowerPoint PPT Presentation

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Page 1: Lauren Avans  Purdue University Calumet, Hammond, IN Faculty Advisor: Young D. Choi

Effect of atmospheric deposition of nitrogen on the fixation and assimilation of nitrogen by wild lupine (Lupinus perennis) in the sand dunes of Lake Michigan

 

Lauren Avans Purdue University Calumet, Hammond, IN

Faculty Advisor: Young D. Choi 

 

Page 2: Lauren Avans  Purdue University Calumet, Hammond, IN Faculty Advisor: Young D. Choi

Introduction• Located at the Lake Michigan Dunes• Karner Blue Butterfly is an endangered species• Sole food source of Karner Blue Butterfly• Increase in Nitrogen Deposition recently• I hypothesize that the addition of nitrogen in the soil will increase the biomass production of wild lupine.  

Page 3: Lauren Avans  Purdue University Calumet, Hammond, IN Faculty Advisor: Young D. Choi

Methods

• Germinated  in dishes and transplanted• Randomly assigned • NH4NO3  Treatment groups: Water, 7.5g m-2 , 15g m-2 

• Harvested 5 months later• Total dry weight biomass and biomass allocation analysis through:– Kruskal-Wallis test– Linear regression analysis

Page 4: Lauren Avans  Purdue University Calumet, Hammond, IN Faculty Advisor: Young D. Choi

Median total biomass of wild lupine seedlings. Results were significant at p<0.05. n=12. * 

represents outliers.

Total dry weight biomass of wild lupine (Lupinus perennis)

  Control Low High

Mean ± SE 224 ± 4 mg 198 ± 4 mg 137 ± 3 mg

Median 201 mg 168 mg 130 mg

Page 5: Lauren Avans  Purdue University Calumet, Hammond, IN Faculty Advisor: Young D. Choi

Median belowground biomass allocation of wild lupine seedlings.  Results were 

significant at p<0.06. n=12. * represents outliers.

Belowground biomass allocation by wild lupine  Control Low High

Mean ± SE 62 ± 5% 60 ± 2% 52 ± 4%Median 66% 63% 53%

Page 6: Lauren Avans  Purdue University Calumet, Hammond, IN Faculty Advisor: Young D. Choi

Linear Regression Line showing a correlation between the amount of biomass allocated belowground (vertical axis) and the amount of total biomass 

(horizontal axis).  

Page 7: Lauren Avans  Purdue University Calumet, Hammond, IN Faculty Advisor: Young D. Choi

Summary

• Contradictory• Population Decline• Total biomass decrease as nitrogen increases • Physiological Drought• Reduction of below ground biomass allocation percent as nitrogen increase 

• Below Ground Biomass Allocation Percent Increases as Total Biomass Increases 

• Allomeric growth vs. Nitrogen increase

Page 8: Lauren Avans  Purdue University Calumet, Hammond, IN Faculty Advisor: Young D. Choi

Acknowledgements

This project was funded by Flora Richardson Foundation

Page 9: Lauren Avans  Purdue University Calumet, Hammond, IN Faculty Advisor: Young D. Choi

Works CitedDriscoll, C., D. withal, J. Amber, E. Boyer, M. Castro, C. Cronan, C. Goodale, P. 

Groffman, C. Hopkinson, K. Lambert, G. Lawrence, and S. Ollinger. 2003. Nitrogen pollution in the northeastern United States: sources, effects and management options. Bioscience 53: 357-374. 

Forrester, J. A., D. J. Leopold, and S. D. Hafner. 2005. Maintaining Critical Habitat in a Heavily Managed Landscape: Effects of Power Line Corridor Management on Karner Blue Butterfly (Lycaeides melissa samuelis) Habitat. Restoration Ecology 13(3): 488-498.

Grundel, R., N. B. Pavlovic, and C. L. Sulzman. 1998. The effect of canopy cover and seasonal change on host plant quality for the endangered Karner blue butterfly (Lycaeides melissa samuelis). Oecologia 114: 243-250.

Grundel, R., N. B. Pavlovic, and C.L. Sulzman. 1999. Nectar Plant Selection by the Karner Blue Butterfly (Lycaeides melissa samuelis) at the Indiana Dunes National Lakeshore. The American Midland Naturalist 114: 1-10.