Ivermectin Effects on Caenorhabditis elegans (C.elegans) Moyosore Olagoke Ivermectin C.elegans

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Text of Ivermectin Effects on Caenorhabditis elegans (C.elegans) Moyosore Olagoke Ivermectin C.elegans

  • Ivermectin Effects on Caenorhabditis elegans (C.elegans) Moyosore OlagokeIvermectinC.elegans

  • Background Information 1.Anti-parasitic MedicationStrongyloides stercoralis Onchocerciasis volvulus Pubic lice infestation Scabies Wuchereria bancrofti 2 Mechanism of action Bind to GABA Channel Hyperpolarization by Cl Permeabilityhttp://www.pnas.org/cgi/content/full/97/6/2674/F2C95H146O28

  • Background InformationPhylum NematodaFeed on OP50 bacteriaPseudocoelomatesThrashing motion (contraction)Female deposit 100,000 or more egg/dayMost individuals are Hermaphrodites

    Life Cycle : >12hours

    Caenorhabditis elegans

  • ResearchPurpose :To determine the effect of Ivermectin on wild type (C.elegans) Plan to extend strain

    Complications Method of introduction Drug Test Resistance strain

  • Ivermectin response to OP50 bacteria Ivermectin has no effect on OP-50 bacteria

    Avermectin is isolated from strepto Avermectins

  • Drug IntroductionIvermectin AgarSample calculation

    Stock 1- 0.1g/1000l in 100% DMSO

    Stock 2- 10l of stock 1 in 9.99ml 1%DMSO

    15l of stock 2 in 8l Agar

    10g/l x 15l= 150g

    150 g/8l = 62.5g/l

  • Wild type C.elegans

  • Ivermectin: Wildtype worm62.5g/lFewer numbersNo apparent movementWorm appears transparent18.75g/lSame conclusionMovement is perceived (~3 worms)

  • ComparisonIvermectinControl

  • Touch insensitive=Tapping

  • Future Analysis

    Growth Assay: Count the number of gravid adult Reflects Pharyngeal function

    Mechanosensation: Touch sensitivity Neurological Response

    Motility assay: Number of worm still thrashing Locomotion

  • References American Society of, Health-System Pharmacists Inc. Patient Drug Information From MedMaster. Maryland: 2007. Jamison, Dean T., and Joel G. Breman. Disease Control Priorities in Developing Countries. 2nd. New York: Oxford University Press, 1993.

    John Keane and Leon Avery Mechanosensory inputs influence Caenorhabditis elegans pharyngeal activity via ivermectin sensitivity genes. Genetics. 2003 May; 164(1): 153162. PMCID: PMC1462566

    Griffiths, Anthony J.F., and Jeffery H. Miller. HumanGenetics. 7th ed. New York: W.H. Freeman,1996.

    Hart, Anne C. "Behavior." Worm Book July 3 2006. 04 April 2008.

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