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Assignment: Application of enzyme in food industry: Submitted by: Muhammad Irfan Nawaz Reg. No. 2005-ag-1885 Msc. (Hons.) Horticulture Submitted to: Miss. Lubna Course No. Biochem 700

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Ass ignm ent :

A p p l i c a t i o n o f e n z y m e i n f o o d

indust ry :

Subm i t ted by :

M u h a m m a d I rf a n N a w a z

Reg . No . 2005 -ag -1885

Msc . (Hons . ) Ho r t i cu l tu re

Subm i t ted to :

M iss . Lubna

Course No . B iochem 700

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Application of Enzymes in Food Industry:

Food industry utilizes a variety of enzymes for processing of various foods, e.g.,

  production of various types of syrups from starch or sucrose (a- and β-amylases,

glucamylase, pullulanase, invertase, and glucose isomerase), meat/protein processing

using proteases, removal of glucose and or molecular oxygen (O2) using glucose oxidase

and catalase, use of lactase in dairy industry and use of enzymes in fruit juice and

 brewing industries. The use of g1cose oxidase and catalase is briefly described heres.

Glucose oxides are obtained from A. Niger and Penicillium. It is a highly specific

enzyme, which catalyzes the formation of gluconic acid from β - D- glucose in two

separate steps, the second step being nonenzymatic spontaneous hydrolysis. Glucose

oxidase is used for the removal of glucose or oxygen from the food stuffs in order to

enhance their storability. Hydrogen peroxide (H2O2) effectively kills bacteria; it can be

eliminated by using catalase.

An important application of these enzymes is in the processing of egg white for use in

 baking industry. The egg white is treated with a mixture of glucose oxidase and catalase;

additional H2O2 (0.1 % w/w) is added to provide sufficient O2 for glucose oxidase action.

The glucose present in egg white is oxidised, and the egg white is dried for use in baking.

Other applications of this enzyme pair are in

(i) removal of O2 from the air present in the head space of bottled and canned drinks, and

(ii) reduction of non enzymic browning in wines and mayonnaises.

Enzymes for the Food Processing, Dairy and Beverage

Industry:

Animal Feed & Pet Care:

We have blends that help provide natural relief to dry or scaly hair coats, skin problems,

digestive disorders, joint difficulties, immune disorders, excessive shedding, weight

 problems, allergies, bloating, lethargy, flatulence, and coprophagia. We also have a

 proprietary blend called Exclzyme®Pet that promotes tissue, muscle and wound healing,

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helps along recovery from soreness and inflammation and is helpful for pets who are

suffering from arthritis and pain. Exclzyme®Pet is ideal for active or athletic pets.

Baking:

Enzymes are rapidly becoming very important to the Baking Industry. Enzyme

supplements are used in baking to make consistently high-quality products by enabling

 better dough handling, providing anti-staling properties, and allowing control over crumb

texture and color, taste, moisture, and volume.

Enzymes are now replacing chemical supplements that were used in the baking industry

to account for these discrepancies. For instance, our enzyme called SEBake-GO can

replace chemical oxidants, such as bromates, used to strengthen gluten.

Dairy:

Specialty Enzymes' SEB-RMP, microbial rennet used in cheese production, is an

effective substitute for calf/cow, goat, sheep or swine-derived rennets. When milk is

treated with milk-clotting proteases or microbial proteases, it causes casein to precipitate

in the form of curd. The curd is separated from other milk ingredients (whey) and further 

 processed to produce soft and hard cheeses. SEB-RMP is a superior option to animal

derived rennets.

Food Processing:

SEB-Brolase and SEB-Prolase rapidly hydrolyze both animal and plant proteins to reduce

or eliminate gel formation on heating. They are used to hydrolyze soy, pea, yeast, maize

and wheat-flour proteins to produce non-bitter, protein hydrolysates. Pre-processed or 

separated plant and milk proteins such as soya isolates and concentrates, casein and whey

can be easily hydrolyzed to produce non-bitter protein hydrolysates suitable for use as

active ingredients in dietary supplements, functional beverages, fermented foods, baked

goods and other foods.SEB-Acid, SEB-Alkaline and SEB-Neutral are different types of protease enzymes used

in food processing applications to break down and increase the solubility, dispersibility

and digestibility of proteins. They also hydrolyze vegetable and animal proteins to

 peptides, polypeptides, and small amounts of amino acids.

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SEBzyme-XL and CelluSEB is used for extraction, for liquefaction of plant materials and

for downstream processing in the food, starch, and alcohol and brewing industries. In the

food industry, these products are used to improve the processing and extraction of wheat

and corn gluten, soybean protein, cereal starch, seed and nut oils, flavors and colors,

coffee, tea and other botanical extracts.

Fruit and Vegetable Juice:

Specialty Enzymes products, LiquiSEB and ExtractSEB, are pectinases that contain

hemicellulase enzyme activities. These products not only increase juice yields, but also

increase the color and health-promoting antioxidants in fruit and vegetable juices

extracted by pressing or decanter centrifuge. By reducing fruit and vegetable mash

viscosity and improving solid/liquid separation, they increase color extraction and juice

volume by as much as 15 percent.

ClariSEB and SEBamyl enzyme solutions speed up filtration and prevent storage or post-

 packaging haze formation by depectinizing and reducing starch in raw juices. Pectin and

starch must be removed from freshly extracted juices prior to filtration, fining and

concentration. Unripe fruits and vegetables can contain as much as 15 percent starch.

ClariSEB pectinase and SEBamyl amylase can reduce starch and pectin in raw fruits and

 juices, thus achieving clear and stable juices and juice concentrates.

Coffee and Tea:Bioclean TC has a blend of natural enzymes that gives manufacturing areas a 100 percent

safe-detergent and alkali-free cleansing. Bioclean TC is dissolved in water and sprayed in

areas that come in contact with leaf material, such as on rolling tables, CTC rollers,

conveyor belts and CFMs. The blend helps degrade biological material, which is then

easily dislodged in cold water washing. Bioclean TC prevents bacterial growth by not

 providing any food to sustain the bacteria. And because it is 100 percent natural, the

 blend doesn’t impart any off-flavor or off-taste to the tea.

Specialty Enzymes, a leading solution provider to the human healthcare, animal nutrition,

and natural product processing industries, offers SPEEDOX. When added to the wash

water or effluent stream in the coffee production process, SPEEDOX rapidly reduces

organic loads in the wastewater that cause environmental pollution.