Thank you for purchasing the Nikon products.
This instruction manual is written for the users of the Nikon’s inverted microscopes “ECLIPSE TE2000-E, ECLIPSE
TE2000-U, ECLIPSE TE2000-S” and describes the basic operations of the microscope.
To ensure correct usage, read this manual carefully before operating the instrument.
• It is prohibited to reproduce or transmit this manual in part or whole without Nikon’s expressed permission.
• The contents of this manual are subject to change without notice.
• Although every effort has been made to ensure the accuracy of this manual, if you note any points that are unclear
or incorrect, contact your nearest Nikon representative.
• Some of the products described in this manual may not be included in the set you have purchased.
• Be sure to read the instruction manual for any other products used in combination with the microscope.
• If you purchased the TE2000-E model, be sure to read the instruction manual supplied with the T-HUBC HUB
• If you are using the TE2000-U/S together with the T-HUBC HUB controller, be sure to read the instruction manual
supplied with the T-HUBC HUB controller.
Warning / Caution symbols used in this manual
Although Nikon products are designed to provide you with the utmost safety during use, incorrect usage or
disregard of the instructions can cause personal injury or property damage and will lead to the forfeiture of all
claims against warranty. For your safety, read the instruction manual carefully and thoroughly before using the
instrument. Do not discard this manual but keep it near the product for easy reference.
In this manual, safety instructions are indicated with the symbols shown below. Be sure to follow the
instructions indicated with these symbols to ensure correct and safe operation.
Disregarding instructions marked with this symbol may lead to death or serious
Disregarding instructions marked with this symbol may lead to injury or property
Meaning of symbols used on the equipment
The symbol appearing on the product indicates the need for caution at all times during use.
Always refer to the instruction manual and read the relevant instructions before manipulating any part to which
the symbol has been affixed.
Caution for heat.
This marking at the top part of the Dia-illuminator and the 12V100W lamphouse calls your
attention on the following;
• Lamphouse becomes very hot during and immediately after the illumination.
• Risk of burns. Do not touch the lamphouse during and immediately after the illumination.
• Make sure that the lamphouse is sufficiently cool before the lamp replacement.
This symbol on the stage calls your attention to the following:
• Spillage of a sample from a vessel onto the microscope presents a biohazard risk.
• To avoid biohazard contamination, do not touch the contaminated portion with your bare
• Decontaminate the contaminated portion according to the standard procedure of your
1. Intended use of the equipment.
This microscope is intended mainly for use in microscopic observation and in the micromanipulation of
living cells and tissue using diascopic (transmitted) and episcopic (reflected) illumination. It is designed
for the main purposes of experimentation and observation, in hospitals or other laboratories, of such cells
and tissue within the fields of genetics, immunology, physiology, pharmacology, neurology, cellular biology,
and molecular biology.
2. Do not disassemble.
Disassembly may cause malfunction and / or electrical shock, and will lead to the forfeiture of all claims
against warranty. Do not disassemble any part other than those described in this manual. If you
experience any problem with the microscope, notify your nearest Nikon representative.
3. Check the Input Voltage.
This microscope uses a power supply for the lamp.
When using the power supply TE-PS30 or TE-PSE30:
Make sure that the input voltage indication on the rear panel matches your regional voltage supply. If the
voltages do not match, do not use the microscope; instead, notify your nearest Nikon representative.
Using the power supply with the wrong input voltage may cause a short circuit or fire. It may also cause
damage to the microscope.
When using the power supply TE2-PS100W:
If you are using the power supply TE2-PS100W, there is no need to check the input voltage since the input
voltage of this power supply is AC 100-240V and can be used at any place in the world.
4. Check the AC adapter of the HUB controller (when using the T-HUBC HUB controller).
The HUB controller is powered by the AC adapter. Be sure to use the specified adapter model meeting the
requirements given below. Use of any other type of adapter can result in malfunction, excessive heating,
• To prevent malfunction and/or fire, be sure to use the AC adapter in a well-ventilated location. To
ensure that it radiates heat properly and does not overheat, never cover or place any object on the
• To prevent malfunction, always turn off the power switch (switch to “O”) of the HUB controller before
attaching the AC adapter.
• Specified AC adapter
Manufacturer: PHIHONG ENTERPRISE (Taiwan)
Model: PSA30U-120 (N)
Rated input voltage: AC 100-240 V, 0.7 A, 50/60 Hz
Voltage fluctuation: ±10%
Rated output voltage: DC 12 V
Rated output current: 2.5 A
Others: UL Listed product, GS approved, CE satisfied
5. Power cord for power supply and the power cord for AC adaptor of the HUB controller
To prevent electric shock, always turn off the power switch (switch to “O”) for the power supply and the HUB
controller before attaching or detaching the power cord. Use one of the power cords specified below. Use of an
improper power cord can result in fire or other hazard. Also note that the power supply is classified as subject
to protection class I against electric shock. Therefore, be sure to connect it to a protective ground terminal.
• Using units in areas where the supply voltage is 100 to 120 V
UL Listed detachable power cord set, 3 conductor grounding Type SVT, No. 18 AWG, 3 m long maximum,
rated at 125 V AC minimum.
• Using units in areas where the supply voltage is 220 to 240 V
Approved according to EU/EN standards, 3 conductor grounding Type HO5VV-F, 3 m long maximum,
rated at 250 V AC minimum.
6. Heat from the light source.
• The lamp and the lamphouse become extremely hot by the lamp illumination. To avoid burns, do not
touch the lamphouse while the lamp is lit or for thirty minutes after it has been turned off.
• To avoid the risk of fire, do not place fabric, paper or highly flammable volatile materials such as
gasoline, petroleum benzine, paint thinner or alcohol near the lamphouse while the lamp is lit or for
about thirty minutes after it has been turned off.
• The bottom plate of the power supply becomes hot during use. Do not cover up the ventilation holes
on the side of the power supply.
Continued on the next page
7. Hazardous sample
This microscope is mainly for use in microscopic observation and micromanipulation of living cells and
tissue cultures in Petri dishes, microtiter plates, etc.
When handling a sample, check to determine whether the sample is hazardous. Handle hazardous
samples according to the standard procedure of your laboratory. If the sample is of an infectious
nature, wear rubber gloves to avoid infection, and be careful not to spill the sample. In the event of
spillage of a sample from a vessel onto the microscope, decontaminate the contaminated portion
according to the standard procedure of your laboratory.
1. Check the combination of the lamp, dia-illuminator and power supply.
The dia-illuminator and the power supply must be used in correct combination against the ratings of the
lamp and the regional voltage supply. See page 38 to find out the correct combination of these items.
Using the equipment in wrong combination will lead to fire, electric shocks or malfunction of the
2. Turn off the power during assembly, connection/disconnection of the cords, and lamp
To prevent electric shocks and/or malfunction, always turn off the power switch of the power supply and
the T-HUBC HUB controller (flip it to the side) and unplug the power cord from the wall outlet before
assembly, connecting or disconnecting of the cords, and the lamp replacement.
3. Cautions on lamp replacement.
To avoid burns, wait at least 30 minutes after the lamp is turned off so that the lamp can cool sufficiently.
To avoid electric shocks and malfunction, always turn off the power switch (flip it to the side) and unplug
the power cord from the wall outlet before lamp replacement.
Securely attach the lamphouse cover to the lamphouse after replacing the lamp. Never light the lamp
while the lamphouse cover is open. Do not break used lamps; instead dispose of them as special
industrial waste or according to the laws applicable to your municipal waste system.
4. Do not wet the microscope.
If the instrument gets wet, a short circuit may result that may cause malfunction or abnormal heating. If
you accidentally spill a liquid on the instrument, immediately turn off the power switch (f