1
tological study, however, showed highly specific binding to lung cancers; MAb 8 bound to 68% of 65 lung cancers, and MAb 15 bound to 72% of them. Interestingly, both antibodies were more reactive with non-small cell than small cell lung cancers and bound most frequently to large cell carcinoma. Most extrapulmonary tumor tissues were negative in staining with a few exceptions; endodermal sinus tumor (two of two) was positive to both antibodies, breast carcinoma (one of five) to MAb 8, gastric carcinoma (one of three), and malignant melanoma (one of one) to MAb 15. Cross-reactions with normal tissues were limited; MAb 8 reacted with adult and fetal lung, and MAb 15 with esophagus and renal tubules. MAb 8 recognized a M(r) 48,000 glycoprotein antigen (carbohydrates as its epitope), and MAb 15 recognized two proteins (M (r) 85,000 and 45,000) (peptides as their epitopes). These two antibodies detecting novel antigens ex- tensively associated with an highly specific to lung cancers, are poten- tially useful for the study of lung cancer. 4. PATHOLOGY Histopathology of Lung Cancer in New Mexico, 1970-72 and 1980-81. Butler, C., Samet, J.M., Humble, C.G., Sweeny, E.S. Department of Pathology, University of New Mexico School of Medicine, Albuquerque, MN 87131, U.S.A. J. Natl. Cancer Inst. 78: 85-90, 1987. In conjunction with a population- based case-control study of lung cancer in New Mexico, the histopathology of cases diagnosed durign 1980 and 1981 and during 1970-72 was reviewed. Adequate histologic or cytologic material was obtained for 725 cases, with 308 during 1970-72 and 417 during 1980-81. The light microscopic his- tologic type was classified on the basis of review by 2 pathologists. No significant differences were found in the histologic-type distributions in Hispanics and non-Hispanics whites. In males, the distributions of histologic types were similar in the two time peirods, but in non-Hispanic white women the proportion of adenocarcinoma declined.during 1980-81 as the propor- tion of small cell carcinoma increased. The panel classification was compared with that recorded by the New Mexico Tumor Registry. Overall agreement was 52.1% for 1970-72 and increased to 65.2% for 1980-81. The discrepancies between the two classifications were largest for the categories of large cell undifferentiated carcinoma and 'other malignancy' 135 Immunohistological Staining of Reactive Mesothelium, Mesothelioma, and Lung Carcinom~ With a Panel of Monoclonal Antibodies. Ghosh, A.K., Gatter, K.C., Dunnill, M.S., Mason, D.Y. Nuffield Department of Pathology, John Radcliffe Hospital, Oxford, U.K.J. Clin. Pathol. 40: 19- 25, 1987. A panel of seven monoclonal an- tiepithelial antibodies of different specificities, including anticytoke- ratin, human milk fat globule membrane, Ca, and carcinoembryonic antigen (CEA) were used with the alkaline phosphatase-antialkaline phosphatase (APAAP) immunostaining technique to determine their value in the differen- tiation between benign and malignant mesothelial cells and lung carcinoma in histological preparations. The an- ticytokeratin antibody reacted strongly with all cases of reactive mesothelium, mesothelioma, and lung carcinoma. An- tibodies to human milk fat globule membrane and the Ca antigen stained mesothelioma and carcinoma and 43% of cases of reactive mesothelium. Staining for carcinoembryonic antigen was not detected in reactive mesothelium or mesothelioma, but was present in most of the lung carcinomas. CEA seemed to be the single most useful marker in distinguishing carcinoma from mesothelioma in that a positive reac- tion for CEA would indicate carcinoma rather than mesothelioma. Limitations of the Usefulness of Microvillous Ultrastructure in Distin- guishing Between Carcinoma Primary in and Metastatic to the Lung. Engstrand, D.A., England, D.M., Oberley, T.D. Middleton Memorial Veterans Hospital, Madison, WI 53705, U.S.A. Ultrastruct. Pathol. ii: 53-58, 1987. We performed ultrasound analysis on 70 consecutive patients with solitary cancers in lung with the following his- tologic classifications: adenocarcinoma (42 cases), bronchioloalveolar car- cinoma (13), large cell carcinoma (4), and adenosquamous carcinoma (ii). Off these 70 cases, nineteen (13 adenocarcinomas, 4 bronchioloalveolar carcinomas, and 2 adenosquamous carcinomas) contained cell surface microvilli with microvillus core root- lets and/or glycocalyceal bodies. Sub- sequent clinical follow-up revealed that three of these 19 cases were ac- tually metastatic colon carcinomas. The remaining 16 patients are currently free of extrathoracic primary disease and are therefore, presumably, primar M carcinoma of the lung. Since both primary and metastatic tumors showed cell surfaces with microvilli having core rootlets and glycocalycean bodies,

Immunohistological staining of reactive mesothelium, mesothelioma, and lung carcinoma with a panel of monoclonal antibodies

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tological study, however, showed highly specific binding to lung cancers; MAb 8 bound to 68% of 65 lung cancers, and MAb 15 bound to 72% of them. Interestingly, both antibodies were more reactive with non-small cell than small cell lung cancers and bound most frequently to large cell carcinoma. Most extrapulmonary tumor tissues were negative in staining with a few exceptions; endodermal sinus tumor (two of two) was positive to both antibodies, breast carcinoma (one of five) to MAb 8, gastric carcinoma (one of three), and malignant melanoma (one of one) to MAb 15. Cross-reactions with normal tissues were limited; MAb 8 reacted with adult and fetal lung, and MAb 15 with esophagus and renal tubules. MAb 8 recognized a M(r) 48,000 glycoprotein antigen (carbohydrates as its epitope), and MAb 15 recognized two proteins (M (r) 85,000 and 45,000) (peptides as their epitopes). These two antibodies detecting novel antigens ex- tensively associated with an highly specific to lung cancers, are poten- tially useful for the study of lung cancer.

4. PATHOLOGY

Histopathology of Lung Cancer in New Mexico, 1970-72 and 1980-81. Butler, C., Samet, J.M., Humble, C.G., Sweeny, E.S. Department of Pathology, University of New Mexico School of Medicine, Albuquerque, MN 87131, U.S.A. J. Natl. Cancer Inst. 78: 85-90, 1987.

In conjunction with a population- based case-control study of lung cancer in New Mexico, the histopathology of cases diagnosed durign 1980 and 1981 and during 1970-72 was reviewed. Adequate histologic or cytologic material was obtained for 725 cases, with 308 during 1970-72 and 417 during 1980-81. The light microscopic his- tologic type was classified on the basis of review by 2 pathologists. No significant differences were found in the histologic-type distributions in Hispanics and non-Hispanics whites. In males, the distributions of histologic types were similar in the two time peirods, but in non-Hispanic white women the proportion of adenocarcinoma declined.during 1980-81 as the propor- tion of small cell carcinoma increased. The panel classification was compared with that recorded by the New Mexico Tumor Registry. Overall agreement was 52.1% for 1970-72 and increased to 65.2% for 1980-81. The discrepancies between the two classifications were largest for the categories of large cell undifferentiated carcinoma and 'other malignancy'

135

Immunohistological Staining of Reactive Mesothelium, Mesothelioma, and Lung Carcinom~ With a Panel of Monoclonal Antibodies. Ghosh, A.K., Gatter, K.C., Dunnill, M.S., Mason, D.Y. Nuffield Department of Pathology, John Radcliffe Hospital, Oxford, U.K.J. Clin. Pathol. 40: 19- 25, 1987.

A panel of seven monoclonal an- tiepithelial antibodies of different specificities, including anticytoke- ratin, human milk fat globule membrane, Ca, and carcinoembryonic antigen (CEA) were used with the alkaline phosphatase-antialkaline phosphatase (APAAP) immunostaining technique to determine their value in the differen- tiation between benign and malignant mesothelial cells and lung carcinoma in histological preparations. The an- ticytokeratin antibody reacted strongly with all cases of reactive mesothelium, mesothelioma, and lung carcinoma. An- tibodies to human milk fat globule membrane and the Ca antigen stained mesothelioma and carcinoma and 43% of cases of reactive mesothelium. Staining for carcinoembryonic antigen was not detected in reactive mesothelium or mesothelioma, but was present in most of the lung carcinomas. CEA seemed to be the single most useful marker in distinguishing carcinoma from mesothelioma in that a positive reac- tion for CEA would indicate carcinoma rather than mesothelioma.

Limitations of the Usefulness of Microvillous Ultrastructure in Distin- guishing Between Carcinoma Primary in and Metastatic to the Lung. Engstrand, D.A., England, D.M., Oberley, T.D. Middleton Memorial Veterans Hospital, Madison, WI 53705, U.S.A. Ultrastruct. Pathol. ii: 53-58, 1987.

We performed ultrasound analysis on 70 consecutive patients with solitary cancers in lung with the following his- tologic classifications: adenocarcinoma (42 cases), bronchioloalveolar car- cinoma (13), large cell carcinoma (4), and adenosquamous carcinoma (ii). Off these 70 cases, nineteen (13 adenocarcinomas, 4 bronchioloalveolar carcinomas, and 2 adenosquamous carcinomas) contained cell surface microvilli with microvillus core root- lets and/or glycocalyceal bodies. Sub- sequent clinical follow-up revealed that three of these 19 cases were ac- tually metastatic colon carcinomas. The remaining 16 patients are currently free of extrathoracic primary disease and are therefore, presumably, primar M carcinoma of the lung. Since both primary and metastatic tumors showed cell surfaces with microvilli having core rootlets and glycocalycean bodies,