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Human Drosophila C. elegans ~ 24,000 Genes ~ 13,000 Genes ~ 19,000 Genes Mouse ~ 24,000 Genes

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Text of Human Drosophila C. elegans ~ 24,000 Genes ~ 13,000 Genes ~ 19,000 Genes Mouse ~ 24,000 Genes

  • HumanDrosophilaC. elegans~ 24,000 Genes~ 13,000 Genes~ 19,000 GenesMouse~ 24,000 Genes

  • Somatic Cell Nuclear Transfer Cloning

  • Transcription Initiation

  • Gene Regulatory Proteins and the DNA SequencesThat They Recognize

  • Asymmetric Localization of Ash1 Transcription FactorDICDAPIAsh1-GFPAsh1 mRNAMother cellDaughter cell

  • Developmental Fate

  • Caenorhabditis elegans959 Somatic cells1000-2000 Germ cells

  • C. elegans Life Cycle

  • Cleavage DivisionsEggDAPI stained embryo

  • Asymmetric Segregation of P GranulesEggImmunostaining of P Ganules

  • Wild Typepar-1 MutantPar (Partition) MutantsDICP granules

  • 2-cell embryoPlasma MembraneCytosol

  • Early L3

  • Early L3Late L3

  • Early L3 Fate Map

  • Vulva-less PhentoypeBag of wormsBag of embryos

  • EGF Pathways of Vertebrates

  • Notch Receptor

  • Two-Signal Model of Vulva Development

  • T.ap Cell Development

  • LIN-14 Mutations

  • Lin-14-GFP WT WormsL1AdultL3L2

  • lin-4(gf)lin-4(lf)LIN-4 Mutations

  • RNAi (RNA Interference)Lin-4 RNALin-4 RISCLin-14 mRNARISC = RNA-Induced Silencing ComplexmiRNA = micro RNA

  • Micro RNAs

  • Drosophila melanogaster

  • AnteriorPosteriorDorsalVentral

  • Cleavage in the Drosophila Embryo

  • APAPLarvaEmbryo

  • 2 hrs.10 hrs.APAP

  • APAP

  • GeneticHierarchy inA-P AxisSpecification

  • Mutant Phenotypes for Maternal-Effect Genes

  • Bicoid mRNA/Protein Distribution

  • Gap GeneExpressionPatterns

  • Bicoid Controls Hunchback (hb) Expression