HPLCHPLC Specialist

　　　　　Chemco Scientific Co., Ltd.

HPLC COLUMNS ChemcosorbChemcobondQUICKSORB

&Application Data

URL ; http://www.chemco.jp/E-Mail ; info@chemco.jp

URL ; http://www.chemco.jp/!E-mail : info@chemco.jp

CHEMCOSORB U-seriesCHEMCOSORB U-series packings are prepared from special silica with extremely large surface area (6nm pore,

600 m2/g). With this property of base material and our modification process, CHEMCOSORB U-series results in

more retentive and unique phase than other bland column. Wide range of chemical phase, ODS, C8, Diphenyl,

TMS, CN, NH2 and Si are available.

CHEMCOSORB 5-ODS-UH has very high carbon content (30%), using high surface area base material and

special modification process. This unique ODS phase has high capacity and resolution, and is particularly

effective not only precise separation of oils, fats and low polarity samples but also highly polar water soluble

samples, which are difficult to discriminate with conventional ODS phase. It is suitable for isomeric separation of

organic compounds and gradient elution of modified amino acids. Comparison of hydrophobic retention for other

brand ODS phase is shown in Fig-1.

Column size : 4.6mmφx150mm.Mobile phase : MeOH/ Water =50/50 (v/v)Flow : 1.0 mL/min, Temp. : 40℃, Detection : UV 254 nm.Sample :1. Uracil, 2. Caffein, 3. Phenol, 4. Methylbenzoate,5. Benzene, 6. N, N-dimethyl aniline, 7. Toluene,8. Phenylacetylacetone

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Mobile Phase:MeCN / Water = 75 /25 (v/v)

Column Size: 150 x 4,6 mm, 5 um

Fig-1. Comparison of chromatographic retention.

CHEMCOSORB!®

CHEMCOSORB, completely porous silica packings, is prepared from M.

Nagel’s Nucleosil 8, 10 and 12 nm silica gel with our unique surface

modification. Wide pore packings are also available for peptide and

protein separation. All of packings are processed our careful end-capping

treatment and give good peak shape for basic solute.

ODS-L allows relatively rapid elution of a wide range of polar to non polar solutes. ODS-H is excellent in both capacity

and resolution and is suitable for conventional separation. Wide range of chemical phase, C8, C4, Diphenyl, TMS, CN,

NH2, OH2( diol ), SCX, SAX, CM and DEAE are available in different particle size.

Completely porous irregular silica, CHEMCOSORB I-series, is ideal tool for economic separation in preparative

chromatography.

URL ; http://www.chemco.jp/!E-mail : info@chemco.jp

CHEMCOSORB 5-ODS-UH, the best material for scale up.

The retentive property of CHEMCOSORB 5-ODS-UH gives advantage in preparative chromatography. As shownin Fig-2, extremely large capacity of CHEMCOSORB 5-ODS-UH enables to load more concentrated sample than

other ODS columns, while maintaining good resolution under over load condition. In preparative

chromatography, CHEMCOSORB 5-ODS-UH is the best alternative to reduce total production cost and achieve

efficient separation.

Mobile phase; 80% MeOH, Column Size ; 4.6 mm I.d. x 150 mm. Flow ; 1.0 mL/min,

UV 300nm (over load injection) , Sample ; 1. Ethyl paraben, 2. Butyl paraben.

Conventional ODS

(350 m2/g, 14%C)

1 µg Injection

(Injection vol. 1 µL)

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(Injection vol. 100 µL)

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Chemcosorb 5ODS-UH

(600 m2/g, 30%C)

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Fig-2. Comparison of peak shape under over load condition.

U-13!Barbiturates

CHEMCOSORB 5ODS-UH

4.6!X150mm

CH3OH/H2O(49:51)

1.0mL/min, 4012 UV

220nm

1.Barbital 2.Allobarbital

3.Aprobarbital

4.Butabarbital 5.butarbital

6.Hexobarbital

7.Pentobarbital

8.Secobarbital

U-26! Nucleotides

CHEMCOSORB 5C8-U

4.6!X250mm

0.1M NH4H2PO4(pH

3.7/H3PO4)

1.5mL/min, 4012 UV 254nm

1.ATP 2.ADP 3.IMP 4.AMP

5.Hypoxannthine 6.Inosine

U-25!Fullerenes

CHEMCOSORB 5ODS-UH

4.6!X150mm

C6H6CH3/CH3CN(60:40)

1.5mL/min, ambient, UV @ 285nm

1. Fullerene C60, 2. Fullerene C70

U-19 34567849:;<=45!>?:@<U-7!Polyaromatic hydrocarbons

U-5!Cold medicine

CHEMCOSORB 5ODS-UH!4.6!X150mm

50mM KH2PO4[pH 2.5/H3PO4]/CH3CN (76:24)

1.0mL/min, 4012 UV 280nm

1.Potassium guaiacol-4-sulfonate

2.p-Acetamidophenol 3.Caffeine

4.d-Chlorpheniramine maleate 5.Salicylamide

6.Acetyisalicylic acid 7.Phenacetin 8.Bucetin

URL ; http://www.chemco.jp/!E-mail : info@chemco.jp

CHEMCOBONDTM

CHEMCOBOND series packings are prepared from ultra pure silica gel that is

obtained by sol-gel technology. Due to its narrow particle size distribution,

CHEMCOBOND series give lower column back pressure than CHEMCOSORB. Peakshape for basic and chelateing solute are improved because of excellent endcaping

and metal free silica gel. ( Chemical phase; ODS, C8, NH2 and OH2 (diol), Particle

diameter; 3.5 µm, 5 µm, Pore diameter; 10 nm )

CHEMCOBOND ODS-W is specially designed ODS phase for polar solute separationin aqueous mobile phase. By optimizing silica pore size and ODS coverage, this

phase allows to use highly aqueous mobile phase for separation of polar solutes with

good retention reproducibility.

0 5 10 15

Tryptic digest of bovine serum albumin.

CHEMCOBOND 5-ODS-W, 4.6!x150mm

2.0 mL min, 0% A-60%B in 15 min.

A: 0.1% TFA, B: 0.1% TFA in Acetonitrile

CHEMCOBOND 3.5-ODS-H

QUICKSORBOur new ODS phase, QUICKSORB (18 nm pore, 200 m2/g, 13% carbon), is

suitable for high throughput HPLC separation. It reduces the actual analysis time in

isocratic elution. In spite of shorter analysis time, the chromatographic performance

is still maintained. In addition, the ultimate end capping and pure silica gel enableexcellent peak shape for basic and/ or chelating samples.

Fig-1 shows the chromatogram of 8 test solutes including basic and chelating

compounds. QUICKSORB separates these compounds with shorter and excellent

peak shape.Quicksorb 3 um

-H

B-8!Nucleosides

CHEMCOBOND 5ODS-H!4.6!X250mm

(22.5mM KH2PO4+2.5mM Na2HPO4)

[pH5.91]/CH3OH(91:9)

1.0mL/min, 4012 UV 260nm

1.Cytosine 2.Cytidine 3.Guanine 4.Uridine

5.Thymine 6.Guanosine 7.Adenine 8.Thymidine

9.Adenosine

B-11!Antidepressant Drugs

B-6!Anticonvalsants

URL ; http://www.chemco.jp/!E-mail : info@chemco.jp

First LC Column

Conventional Column

I.D., 3.0, 4.0, 4,6, 6,0 mm

Semi-Micro Column

I.D., 1.0, ( 2.0 ), 2.1 mm

Semi-Prep Column

I.D., 7.5, 10.0 mm

Prep ColumnI.D., 20.0, 30.0 mm

HPLC COLUMNS

Table1. HPLC phase specifications.

Chemcopak HPLC Columns

We can pack any size of column.

(Diameter 1.0 mm - 30 mm, column length

10 – 500mm.)

In addition to conventional size column,

micro capillary columns (diameter 0.1,

0.32, 0.53mm) are available for specific

application. These columns are sold by

name of “chemcopak e-series”.

HORIKAWA BLDG.,

2-8-14,NAKAZAKI, KITA-KU, OSAKA, JAPAN

Tel: +81-6-6375-0395!Fax: +81-6-6372-0533

Particle Pore Surface Carbon Particle Pore Surface CarbonDiameter Size Area Content Diameter Size Area Content（μm） (nm) (m2/g) (%) （μm） (nm) (m2/g) (%)

QUICKSORB 3, 5 18 200 13 Si-U 5, 10 ----ODS-H 3.5, 5 10 340 22 C8-U 5, 10 14ODS-W 5 13 300 15 ODS-UH 5, 10 30C8 5 10 340 ---- Dph-U 5 ----NH2 3.5, 5 10 340 ---- CN-U 5 ----OH(Diol) 5 10 340 ---- NH2-U 5 ----Si 5, 7 8 ---- SCX 7 8 450 ----C8 7 8 9 SAX 7 8 450 ----ODS-L 5, 7, 10 10 10 300-CM 7 30 100 ----ODS-H 5, 7, 10 10 20 300-DEAE 7 30 100 ----Dph 5, 7 8 7 300-Si 7 100 ----TMS 5, 7 8 5 300-C4 7 100 0.8NH2 5, 7 8 ---- 300-C8 7 100 2.5CN 7 8 ---- 300-ODS-L 7 100 4120-Si ---- 300-C18 5, 7 30 100 6C8 8 300-Dph 7 100 1.2ODS-L 9 300-CN 7 100 ----ODS-H 18 300-NH2 7 100 ----Dph 6 300-OH 7 100 ----TMS 4 I-Si ----CN ---- I-C18 10NH2 ----

250

Column

CHEMCOSORB

6 600CHEMCOSORB

12

450

200

5, 7, 10 10

CHEMCOSORB

Column

CHEMCOBOND

3

Specialist

URL ; http://www.chemco.jp/E-Mail ; info@chemco.jp