HPLC * % ( ) ˙ ˚ ˜ - core.ac.uk ˘ˇˆ ˙˝˛˚˜˛˘ˇ = I (˙>I8I I I˘ (Licorice) ˛˚˜ $˘ #

  • View
    214

  • Download
    0

Embed Size (px)

Text of HPLC * % ( ) ˙ ˚ ˜ - core.ac.uk ˘ˇˆ ˙˝˛˚˜˛˘ˇ = I (˙>I8I I I˘ (Licorice) ˛˚˜ $˘ #

!"#$%'('#)*

!"#$ %& #'

() %*HPLC !"#$%

(Glycyrrhiza glabra L.)

! "# $%& ' %(&) *"# (+ ,- $.$ /0(*123+4'56#'7'/0(+/8$

!09&:(&);'88'8&?8*

@A#8'8&:(&);'8@

!

=

(Licorice)I(>I8III

Glycyrrhiza glabra L.*#$ &6cS II$6L I

$%&':(&)* I;$$K7,$d%&cI*I99;

f/4[SQ#!I4[6'6#&b&8*I(

& 'e[8I7g' 6&06+'[08 *'%

,!?&8,- Q&08Ehi*O'

.'8/I.'S/.ihd;7'$OhC%jbiBV.@*F

7k*$H&4;'86*7

I Ic6d;$IIAI@MI7&8*1I$IISIl I&N'67.8N'67m(7!J&0

*"# 8'3 .n'63/0(+')@\/0(0

cMTVTTI6&0

#II(II+,'IIII.$IIIIII/0II(a/0I(*d+'Ib$,-/

k&8oB&FEhiQ&01Oh.&!I9p8I6L 8qAD+

& VTV\I+IBZrV*Fa8''(H'k(a/0(/K

H'kK'aa0(!*./0(a8a/0(/60(.&3 *

I(>'I8'!aI8I?/0(a/0*OkI/0I(&0IH'=

$!a$*#;ZW.DW/'(AUMr\U@W/8B@.\*F

!"##$%%%'()*+"%,*%-.+/.012*3

1AT3oH/0( IqCI$%&/'IBMF1II

@TTTbH$%&I$I$* ,$P .S) !NI&KIlIS/8$4b

q!l8B&F%&II$'I*$>?8 /0I(I$#8

IIIa/0III(RNADNA III4P.'#Iv4b :(&)C'8$'I/58w'b

8'6:(&);'8!"#$,-*

"#%0#"##$%& 45%6 *#

>

'56 123>$/x)#

/0(5(]4/8$&I:(&);'8

S;7*+$+=SI8'8OI+;/I0( &I$II

8'8G7 I?8 I&`'I8'8$63d'+ II$>?8 hBM.V\.VMF*IdIt"#4t

!

?

SHIM ADZA-10AVPC'I88'&IISHIM PACK-CLC-C84mmAU\cm@MdEM$'I&k

$%&''II&kUV'IH'IS@M\.&'8I8 I1!>?8 &0m'a/*

RI/I?;H{I;I7I.jIh&=

IIcBO&8'&I@MWH'8I&.\MWN!@DW

H{a&@+F.V('9&K*I8'8JZIRI/I*I$Q&I(b 8&&6BV\.VM.VY*F

%#&$HPLC5(]& ZT.\T.MT.AT.YT.DT>k

(/0(8&&KB`'Sigmaw'(b"7.>'8'!(.k!YMFWN!H{;

[08H'8&VV$Q&HPLCIR/B8'8ZFQ8&(=$%&sq@UT

Kl+ &kVTT(I&) &KI*sq@T(=t!H'8&N!H{;&K

'IIb&(=_{8'8!O*KOb8'8?;8VTT(I$8&K*

sq8'8a$Q&HPLC8R/6h6>'6h6E$%&

[h8'8/0(9'[K*sq4a/0(+H'=$%&

B@rTVY.\F

Bm

A a/0(+

A>c0;d/0(>'8'!'8'5(]VTT('I[KI6h6$%&I8'8&K

$[h*Bd/0(>'8'!'8'8&w'(b+m>c0;8'8

D@@a/0(K'kK'

D\TN!d/0(>'8'!'8'K'kK''I!.yI&8 !O(h/? /KI8!

8'!=Sast8'8$%& *

'/0(5(]/@D'8'8

Ok@>'8'8a$Q&O+; HPLCOkZ$$#88*$I+/

O+;$I/0I(+/8H'8 $$4$Q&I(HPLCI`'I@D

$#8'84KE'6L+$IOI+;a#b$/JI I

/8(0'/0(+a#b$8'8$H7V$$!*

$JO+;$/H'8II B(0'F$JiIIE9N!

$%&0kMj8'8=d'+8N)&8.09*J$IQ8I

$H'8II XTUZXAUVAJ>I$E9N! D@UVYAUY>I!

BH7@*F

"#%0#"##$%& 45%6 *#

@

()

*!"##$%&789:;,60

+!&+ HPLC006I[7IBYMUTX\U\F8BH7A*FK; /0I(I9

8'8

$%& 45"#%0#"##%6 *#

%

:II(&);'II8IIII@UAII@UVTWJ/II$BVM*FJ/ILauren/0I(I9

$!ZUVVUYBWi'&YUAFW$II

B@V*F09I8'8OII+;/I0I(I+ I

G7:(&);'8$ !III7; '3$!BMFO+;+

/0(9t;#8IIIe`98[08&;'8P+

#8BTMUTP$9

8'8 !N!P_&[08C%;'8$

a6b '64]'SC%;'8N!03I> 'I*/0I(I5(]

8'8a6b 'N!G%_&[08;'8 'SII64 'II8IIII;>IICII%BTMUTP$l{&bI I8'$8#8

!

A

The Percent of Extract and Glycyrrhizin Content of Glycyrrhia glabra Root Grown in Kerman

Province and Some Samples from Fars Province by HPLC Method

1. M.Sc. student of Plant Biotechnology, International Center for Science & High technology & Environmental Sciences, Kerman,

Iran

2. Associate professor of Genetics & Plant Biotechnology, International Center for Science & High technology & Environmental

Sciences , Kerman, Iran

3. Associate professor of Pharmacognosy, Pharmaceutics Research Center, Kerman University of Medical Sciences, Kerman. Iran

4. Associate professor of Plant Cytogenetic, Tehran Payame Noor University, Tehran, Iran

* Corresponding author, e-mail: neda.d.p@gmail.com

Abstract Background & Aims: Licorice (Glycyrrhia glabra L.) is an important herbal medicine that its root extract

has long been used for the treatment of various diseases. The essential component of its root is glycyrrhizin.

This study was performed to determine the percentage of the extract and glycyrrhizin content of the roots of

Glycyrrhiz glabra grown in different areas of Kerman province and some samples in Fars province.

Methods: Twenty six samples from the roots of Glycyrrhia glabra grown in various areas of Kerman

province and 2 samples from Fars Province (a total of 28 samples from 8 regions) were collected and

extracted by using ethanol and distilled water (for 5 samples). The percentage of glycyrrhizin in the extracts

was determined by High Performance Liquid Chromatography (HPLC) technique.

Results: Mean percent of extracts from the samples of Fasa and Shiraz in Fars province (18.25 1.06%),

Sirjan (17.29 0.90%) and Bardsir (16.33 5.62%) were higher than other areas (P

$%& 45"#%0#"##%6 *#

References 1. Majnoon Hosseini N, Emami S.D.

Cultivation and Production of Certain Herbs

and Spices. Tehran, University of Tehran

Press, 2007; pp232-5 [Persian].

2. Omidbaigi R. Production and Processing of Medical Plants. Mashhad, Behnashr Co.

2005; pp267-75 [Persian].

3. Salehi Surmaghi M.H. Medical Plants and

Phylothrapy. Tehran, Donyayeh Taghziyeh,

2006; pp257-26 [Persian].

4. Tavasoly V. Persian translation of High Perfermance Liquid Chromatography by

Mayer V.A. Tehran, University Center

Press, 2006 [Persian].

5. Kumada H. Long-term treatment of chronic hepatitis C with glycyrrhizin [Stronger Neo-

Minophagen C (SNMC)] for preventing

liver cirrhosis and hepatocellular carcinoma.

Oncology 2002; 62: 94-100.

6. Hirabayashi K, Iwata S, Matsumoto H, Mori

T, Shibata S, Baba M, et al. Antiviral

activities of glycyrrhizin and its modified

compounds against human immuno-

deficiency virus type 1 (HIV-1) and Herpes

simplex virus type 1 (HSV-1) in vitro. Chem

Pharm Bull (Tokyo) 1991; 39(1): 112-5.

7. Su XS, Chen HM, Wang LH, Jiang CF, Liu JH, Zhao MQ, et al. Clinical and laboratory

observation on the effect of glycyrrhizin in

acute and chronic viral hepatitis. J Tradit

Chin Med 1984; 4(2): 127-32.

8. Akamatsu H, Komura J, Asada Y, Niwa Y.

Mechanism of anti-inflammatory action of

glycyrrhizin: effect on neutrophil functions

including reactive oxygen species

generation. Planta Med 1991; 57(2): 119-21.

9. Tanaka A, Horiuchi M, Katsummi U, Shibamoto T. Antioxidant and anti-

inflammatory activities of water distillate and

its dichloromethane extract from licorice root

(Glycyrrhiza uralensis) and chemical

composition of dichloromethane extract.

Journal of the Science of Food and

Agriculture 2008; 88(7): 1158-65.

10. Nishino H, Kitagawa K, Iwashima A.

Antitumor promoting activity of glycyrrhetic

acid in mouse skin tumor formation induced

by 7, 12-dimethylbenz [a] anthracene plus

teleocidin. Carcinogenesis 1984; 5(11):

1529-30.

11. Liu W, Kato M, Akhand A, Hayakawa A,

Takemura M, Yoshida S, et al. The herbal

medicine Sho-saiko-to inhibits the growth of

malignant melanoma cells by up-regulating

Fas mediated apoptosis and arresting cell

cycle through downregulating of cyclin

dependent kinases. Int J Oncol 1998; 12:

1321-6.

12. Nassiri Asl M, Hosseinzadeh H.M. Review

of the Pharmacological Effects of

Glycyrrhiza sp, and its Bioactive

Compounds. Phytother Res 2008; 22(6):

70924.

13. Ploeger B, Mensinga T, Sips A, Seinen W, Meulenbelt J, DeJongh J. The

pharmacokinetics of glycyrrhizic acid

evaluated by physiologically based

pharmacokinetic modeling. Drug Metab Rev

2001; 33(2): 125-47.

14. Hagimahdipoor H, Ahanzadeh Y, Hassanlow T, Shakarchy M, Abedi Z, Pirali

M. Study of the liquorice roots that have

been collected from different areas of Iran. J

Herb Med 2008; 27(3): 10614 [Persian].

15. Davarpanah Z, Sheikh-Zeinodin M, Dokhani

SH, Saeidi GH. Effects of harvesting season

and location on the suger, ash and

Glycyrrhizic acid content of Licorice root. J

!

=

Sci Technol Agric Natur Resour 2008;

13(47): 27-35 [Persian].

16. Sheidai M, Cyto-morphological Studies of the Genus Glycyrrhiza in Iran. Cytologia

2008; 73(3): 3339.

17. Jiang Y, Lu H.T, Chen F. Preparative

purification of glycyrrhizin extracted from

the root of liquorice using High-speed

counter-current chromatography. J

Chromatogr A 2004; 1033(1), 183-6.

18. Sabbioni C, Mandrioli R, Ferranti A, Bugamelli F, Saracino M.A, Forti G.C, et al.

Separation and analysis of glycyrrhizin, 18-

glycyrrhetic acid and 18-glycyrrhetic acid

in liquorice roots by means of capillary zone

electrophoresis, J Chromatogr A 2005;

1081(1): 65-71.

19. Sabbioni C, Ferranti A, Bugamelli F, Forti G.C, Raggi M.A. Simultaneous HPLC

analysis, with isocratic elution, of

glycyrrhizin and glycyrrhetic acid in

liquorice roots and confectionery products

Phytochemical Anal 2006; 17(1)