Efficiency and Flexibility at Every Step of Real-Time PCR

High-Throughput Real-Time PCR Solutions

Real-Time PCR

BIO-RAD LABORATORIES, INC. | 32 | BIO-RAD LABORATORIES, INC.

HIGH THROUGHPUT. FLEXIBILITY. RELIABILITY. Bio-Rad’s high-throughput real-time PCR products leverage more than 30 years of expertise to support your application and workflow.

■■ Genetic Disease Research■■ Oncology Research■■ Stem Cell Research■■ Pharmacogenomics■■ Pathogen Detection■■ Agricultural Biotechnology■■ Lead Screening■■ Lead Optimization■■ Target Discovery■■ Target Validation■■ Biomarker Studies■■ CRISPR Validation

BIO-RAD LABORATORIES, INC. | 3

GENE TARGET SELECTION

SAMPLE PREPARATION

REACTION SETUP

DATA ACQUISITION

CFX Maestro SOFTWARE ANALYSIS REPORT

GENE TARGET SELECTION

ASSAY DESIGN

ASSAY VALIDATION

PLATE DESIGN

SAMPLE PREPARATION

REACTION SETUP

PROTOCOL SELECTION

DATA ACQUISITION

DATA ANALYSIS

RESULTS

VS.5 STEPS

BIO-RAD WORKFLOW

10 STEPS

TRADITIONAL WORKFLOW

PrimePCRGene Expression

Assays

PrimePCRRun Files

PrimePCRGene Expression

Assays

PrimePCRRun Files

HIGH THROUGHPUT

MAXIMIZED THROUGHPUT. REDUCED MANUAL INTERVENTION.Traditional real-time PCR workflows involve multiple unreliable and labor-intensive steps that limit throughput, process efficiency, and scalability. We provide a suite of products that simplifies and accelerates real-time PCR to permit true high-throughput gene expression profiling and quantification.

4 | BIO-RAD LABORATORIES, INC.

Bio-Rad Competitor

vs.

CFX Automation System II

1CFX Real-Time PCR Detection System

2CFX Real-Time PCR Detection System

43 in. (109 cm)

Competitor AutomationRobot

1Competitor Real-Time PCR System

Competitor AutomationRobot

2Competitor Real-Time PCR System

97 in. (246 cm)

30 in

. (76

cm

)

PrimePCR run files

3 Complete statistical analysis with CFX Maestro Software

2 Load run files into automation software

1 Download run files

Quantitative PCR

Needs Standard Throughput Large Reaction Volume High Throughput

Reaction Module CFX96 CFX96 Deep Well CFX384

Interchangeable Module

Flexible System Configurations

CFX Real-Time PCR Detection Systems and CFX Maestro Software provide:■■ Laboratory information management system

(LIMS) and automation compatibility■■ Barcode-specific cycling protocols that can

be assigned to each plate, with or without LIMS integration

■■ CFX Maestro Software, Security Edition to support U.S. FDA 21 CFR Part 11 regulations

■■ Interchangeable 96- and 384-well reaction modules for real-time PCR

■■ Customized run setup and data analysis ■■ Multi-instrument connectivity■■ 5-target multiplexing■■ Remote notification of run completion with

attached data file

HIGH THROUGHPUT

Twice the throughput. Half the footprint.The CFX Automation System II can operate two CFX Real-Time PCR Detection Systems simultaneously, doubling throughput and saving valuable benchspace, compared to other automation systems.

From Plates to Analyzed Data in Three Clicks

Benchspace Layout

BIO-RAD LABORATORIES, INC. | 5

From tissue culture to real-time PCR without time-consuming RNA purification steps. Prepare real-time PCR–compatible cell lysates in a single 10 min step using SingleShot Cell Lysis RT-qPCR Kits.

Comprehensive Portfolio of Consumables

Our line of robotics-compatible real-time PCR consumables was designed specifically with automation in mind. Products include:■■ Temperature-stable supermixes■■ Customizable, warp-free Hard-Shell PCR Plates and accessories■■ One-step reverse transcription quantitative PCR (RT-qPCR) kits■■ Kits for RT-qPCR directly from lysate

FLEXIBLE AND CUSTOMIZABLE

Supermixes for Every Sample

Our SsoAdvanced Supermixes utilize patented* Sso7d fusion polymerase technology, which increases processivity and inhibitor tolerance to ensure superior performance even on challenging samples. Supermixes are available as custom formulations and in custom sizes. Products include:■■ SsoAdvanced Universal SYBR® Green and Probes Supermixes■■ SsoAdvanced Universal Inhibitor-Tolerant SYBR® Green Supermix■■ SsoAdvanced PreAmp Supermix■■ Reliance One-Step Multiplex Supermix■■ SingleShot Cell Lysis RT-qPCR Kits

FLEXIBLE SYSTEMS. CUSTOMIZABLE ASSAYS.Real-time PCR reagent, instrument, and software requirements are as diverse as the products they help innovate. To address this, we have built flexibility, customizability, and time-saving solutions into our entire product line to maximize your throughput and workflow.

Reliable real-time PCR data from even the most challenging samples. SsoAdvanced Universal Inhibitor-Tolerant SYBR® Green Supermix is a high-performance real-time PCR supermix that allows generation of superior data using crude lysates from plants, seeds, cells, bacteria, and formalin-fixed paraffin-embedded samples.

Customizable Real-Time PCR Assays and Arrays

Bio-Rad’s PrimePCR Gene Expression Assays are expertly designed, and panels are curated to include only biologically relevant genes. PrimePCR Assays are available as:■■ SYBR® Green– and probe-based

individual assays■■ Custom predesigned disease and

pathway panels■■ Wet-lab validated and next-generation

sequencing verified assays for the human, rat, and mouse genomes

■■ Expertly in silico–designed assays or a range of other model organisms

* U.S. patents 6,627,424; 7,541,170; and 7,560,260.

6 | BIO-RAD LABORATORIES, INC.

INSTRUMENT VALIDATION AND QUALIFICATIONBio-Rad provides a comprehensive suite of products and services that ensures your instruments are cared for and perform to our standard of quality, run after run.

REGULATORY TOOLS AND SERVICES

On-Site Thermal Validation Services

To ensure optimal performance of our CFX Real-Time PCR Detection Systems, a Bio-Rad trained and certified service engineer will:■■ Provide a thermal validation report clearly

documenting instrument performance ■■ Assess PCR block accuracy, thermal

uniformity, and instrument ramp rate■■ Confirm thermal block settling times and

overshoot are within specifications

CFX Qualification Plate

The CFX Qualification Plate provides a reliable and fast way to qualify any CFX Real-Time PCR Detection System. These sealed and frozen instrument qualification plates contain predispensed reagents and offer: ■■ 1-click pass/fail results■■ Easy integration into quality control procedures■■ Convenient troubleshooting

Bio-Rad’s Expert Care Service

Bio-Rad offers a no-contract Repair Plan and a wide variety of individualized service plans.

■■ Choose a preconfigured plan or design your own custom plan with MyPlan for maximum flexibility

■■ Use on-site and off-site services as needed■■ Consult our team of dedicated experts who have direct access

to the latest procedures, parts, tools, and factory-trained engineers to ensure the best service for your instruments

4 Thermal Validation Services 4 IQ/OQ 4 Qualification Plates 4 Expert Care Services

On-Site Installation Qualification (IQ) and Operational Qualification (OQ) Services

A certified service engineer documents that instruments are installed and functioning properly and ensures that instruments will generate reliable and reproducible data.

Care Plan Entitlements • On-site service option • Service center option

Service Complete Support Plan Proactive Plan Performance Plan Extended Coverage Plan

Repairs • • • •PM1 • • • • • •Parts (repair) • • • •Shipping2 • • •Labor3 • • • • • • • •Travel • • • •Priority response4 • • • •Remote diagnostics5 • • • • • •Technical support • • • • • • • •Instrument check • • Loaner • •Part discount6 • •

1 The number of preventive maintenance (PM) visits will be in line with manufacturer’s recommendations. Additional PM visits can be added at agreed-upon price levels. PM is not available in all locations. 2 Shipping paid by Bio-Rad (not available in all regions). 3 Labor and travel covered for preventive maintenance and performance check activities only with the Proactive and Performance Plans, respectively. 4 Priority response guaranteed over non-contract customers. 5 Go to bio-rad.com/support/RemoteDiagnosticServices to learn more about remote service and support. 6 Discount applies for service performed by Bio-Rad outside of the PM service. Note: Services may vary by region. Please contact your local Bio-Rad representative for details of availability.

Visit bio-rad.com/support/expertcare for more information.

BIO-RAD LABORATORIES, INC. | 7

20YEARS IN REAL-TIME PCR 30+YEARS IN PCR

Expertise

1,000sOF CFX SYSTEMS INSTALLED IN 80+COUNTRIES

Reliability

#1MARKET LEADER IN DIGITAL PCR #2MARKET LEADER IN REAL-TIME PCR

1,000sOF Sso7d PUBLICATIONS

Innovation

RELIABLE PERFORMANCE. REPRODUCIBLE RESULTS.From the solid-state optical technology that allows our real-time PCR detection systems to perform optimally year after year to the algorithms that ensure that all of the most relevant gene targets are represented in each PrimePCR Panel, our real-time PCR portfolio provides you confidence in reagents, technology, and results.

CONFIDENCE IN RESULTS

Bulletin 6629 Ver C US/EG 19-0152 0319 Sig 0119

Web site bio-rad.com USA 1 800 424 6723 Australia 61 2 9914 2800 Austria 43 01 877 89019 Belgium 32 03 710 53 00 Brazil 55 11 3065 7550 Canada 1 905 364 3435 China 86 21 6169 8500 Czech Republic 36 01 459 6192 Denmark 45 04 452 10 00 Finland 35 08 980 422 00 France 33 01 479 593 00 Germany 49 089 3188 4393 Hong Kong 852 2789 3300 Hungary 36 01 459 6190 India 91 124 4029300 Israel 972 03 963 6050 Italy 39 02 49486600 Japan 81 3 6361 7000 Korea 82 2 3473 4460 Mexico 52 555 488 7670 The Netherlands 31 0 318 540 666 New Zealand 64 9 415 2280 Norway 47 0 233 841 30 Poland 36 01 459 6191 Portugal 351 21 4727717 Russia 7 495 721 14 04 Singapore 65 6415 3188 South Africa 36 01 459 6193 Spain 34 091 49 06 580 Sweden 46 08 555 127 00 Switzerland 41 0617 17 9555 Taiwan 886 2 2578 7189 Thailand 66 2 651 8311 United Arab Emirates 971 4 8187300 United Kingdom 44 01923 47 1301

Bio-Rad Laboratories, Inc.

Life ScienceGroup

Bio-Rad, Hard-Shell, and Microseal are trademarks of Bio-Rad Laboratories, Inc. in certain jurisdictions.

Hard-Shell Plates are covered by one or more of the following U.S. patents or their foreign counterparts owned by Eppendorf AG: U.S. Patent Numbers 7,347,977; 6,340,589; and 6,528,302.

SYBR is a trademark of Thermo Fisher Scientific Inc. Bio-Rad Laboratories, Inc. is licensed by Thermo Fisher Scientific Inc. to sell reagents containing SYBR Green I for use in real-time PCR, for research purposes only.

All trademarks used herein are the property of their respective owner.

Ordering Information

Catalog # Description

Instruments1845075 CFX Automation System II for Real-Time PCR Systems,

includes plate handler, barcode scanner, mounting plate, automation software

1855195 CFX96 Touch Real-Time PCR Detection System, includes C1000 Touch Thermal Cycler Chassis, CFX96 Optical Reaction Module, cables

1855485 CFX384 Touch Real-Time PCR Detection System, includes C1000 Touch Thermal Cycler Chassis, CFX384 Optical Reaction Module, cables

1854095 CFX96 Touch Deep Well Real-Time PCR Detection System, includes C1000 Touch Thermal Cycler Chassis, CFX96 Deep Well Optical Reaction Module, cables

1814000 PX1 PCR Plate Sealer, includes heat sealing instrument, plate support block that holds 96- and 384-well plates, sealing frame, power cord

Reagents and Consumables1725085 SingleShot SYBR® Green Kit for Cell Lysis and RT-qPCR,

100 x 50 µl reactions

1725090 SingleShot Probes Kit for Cell Lysis and RT-qPCR, 100 x 50 µl reactions

1725271 SsoAdvanced Universal SYBR® Green Supermix, 5 x 1 ml vials, 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, SYBR® Green I, universal reference dye, for 500 x 20 µl reactions

1725281 SsoAdvanced Universal Probes Supermix, 5 x 1 ml vials, 2x qPCR mix, contains dNTPs, Sso7d fusion polymerase, MgCl2, stabilizers, universal reference dye, for 500 x 20 µl reactions

1725151 iTaq Universal SYBR® Green One-Step Kit, 5 x 1 ml vials, one-step RT-PCR kit, includes 2x one-step SYBR® Green reaction mix containing iTaq DNA Polymerase, buffers, and universal reference dye; iScript Reverse Transcriptase; and nuclease-free water, for 500 x 20 µl reactions

12010220 Reliance One-Step Multiplex Supermix, 5 ml (5 x 1 ml vials), 1,000 x 20 μl reactions, 4x RT-qPCR supermix, contains Reliance Reverse Transcriptase, blend of high-performance DNA polymerases including Sso7d fusion polymerase, universal reference dye, stabilizer, and reaction buffer, for 1,000 x 20 µl reactions

12010221 Reliance One-Step Multiplex Supermix, 10 ml (2 x 5 ml vials), 2,000 x 20 μl reactions, 4x RT-qPCR supermix, contains Reliance Reverse Transcriptase, blend of high-performance DNA polymerases including Sso7d fusion polymerase, universal reference dye, stabilizer, and reaction buffer, for 2,000 x 20 µl reactions

MSB1001 Microseal 'B' Adhesive Seals, optically clear seals for PCR plates, 100

HSP3905 Hard-Shell 384-Well PCR Plates, thin-wall, skirted, clear shell/white well, barcoded, 50

HSP9901 Hard-Shell 96-Well PCR Plates, low-profile, thin-wall, skirted, white shell/clear well, barcoded, 50

HSP9955 Hard-Shell 96-Well PCR Plates, low-profile, thin-wall, skirted, white shell/white well, barcoded, 50

1845098 CFX Qualification Plate, predispensed 96-well plate for performance validation

1845099 CFX Qualification Plate, predispensed 384-well plate for performance validation

Catalog # Description

Software12004110 CFX Maestro Software, Windows Edition

12004128 CFX Maestro Software, Mac Edition

12005259 CFX Maestro Software, Security Edition, 5 licenses

Visit bio-rad.com/web/qPCRHighThroughput for more information.

Want to order custom PCR primer or probe assays? Let our search tools find the right assay for you!

Visit bio-rad.com/web/PrimePCR for more information.