Abstracts 115 ~]41 HETEROGENEITY OF HLA-DR4 HAPLOTYPES IN THREE ETHNIC GROUPS. X Gao, MA Fernandez-Vina, ME Moraes, E Gazit, C Brautbar, 10.1 Y Sun, J An and P Stastny, Dept of Internal Medicine, UT Southwestern Med Cntr, Dallas, TX, Tel Hashomer, Israel and Haddassah Univ Hosp, Jerusalem, Israel. There are 8 known variants of DR4-DRBI which associate with DQw7 or DQw8 in Caucasians and with DQw4 in Orientals. Little is known about the distribution of these variants or their DQ associations in other ethnic groups. In the present study we have analyzed 59 N.A. Caucasians, 44 Israeli Jews and 25 Chinese from North China, all having DR4. DR4-DRBI genes were amplified with group-specific primers. Locus-specific primers were used for DQAI and DQBI. The dominant DRBI subtypes were totally different in each race. Dw4 dominated the American Caucasian DR4 panel (55.9%), DwI0 was the major type of DR4 in Israeli Jews (59.1%) while in Chinese we found mainly KT2 (DRBI*0406, 36%) and Dwl5 (DRBI*0405, 32%). Each of these alleles was rare in the other ethnic groups. DQAI*0301 was found in virtually all DR4 haplotypes while associations with DQBI alleles were variable in different panels. In all 17 DRBI- DQBI haplotypes were determined and confirmed by family studies. Several of these were not previously described: DRBI Dw DQ Race DRBI Dw DQ Race 0403 13.1 w4 Jew 0408 14.2 w2 Jew 0404 14.1 w4 Jew 0405 w15 w2 Jew 0401 4 w2 Jew 0405 w15 w8 Cau, Jew These results may be important in transplants and in the interpretation of disease associations in different races. ~142 FOUR-LOCUS HLA CLASS II HAPLOTYPES DEFINED BY PCR AND OLIGONUCLEOTIDE HYBRIDIZATION IN FIVE ETHNIC GROUPS. MA I0.| Fernandez-Vina, X Gao, ME Moraes, Y Sun, J An, Z Layrisse and P Stastny, UT Southwestern Med Cntr, Dallas, TX, and IVIC, Caracas, Venezuela. We have analyzed the associations of class II alleles in 480 unrelated individuals including N.A. Caucasoids, Blacks, Latin American, Chinese, and Israeli Jews. Thirty-nine alleles of DRBI, 4 of DRB3, 4 of DRB5, 8 of DQAI, and 14 of DQBI yielded 20 DQAI-DQBI combinations, 65 DRBI-DQAI-DQBI and 71 DRB3/5-DRBI-DQAI-DQBI class II haplotypes, many of which were found only in one ethnic group. The 20 DQAI-DQBI groups and their DR antigens were: DQAI DQBI DR DQAI DQBI DR 0101 0501 l,wl0,wll,wl2 0301 0201 4,7,9 0503 w14 0301 4 0102 0501 wll,wl3,wl5 0302 4,w8 0502 wll,wl3,wl6 0401 4 0602 wll,wl5 0303 9 0604 w13 0401 0402 w8,w18 6.5 w13 0501 0201 w17 0103 0601 w8,w15 0301 l,w8,wll,wl2,wl3, 0603 wlI,wI3 Wl4,Wl6,JX6 0201 0201 7,w13 0601 0301 w8,w12 0303 7 Conclusions: a) there are multiple sites for recombination in the D region; b) these sites are characteristic for haplotypic combinations; c) maintenance of DQAI-DQBI associations could be due to selective advantage or difficulty in pairing with other alleles.

Heterogeneity of HLA-DR4 haplotypes in three ethnic groups

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Page 1: Heterogeneity of HLA-DR4 haplotypes in three ethnic groups

Abstracts 115

~]41 HETEROGENEITY OF HLA-DR4 HAPLOTYPES IN THREE ETHNIC GROUPS. X Gao, MA Fernandez-Vina, ME Moraes, E Gazit, C Brautbar,

10.1 Y Sun, J An and P Stastny, Dept of Internal Medicine, UT Southwestern Med Cntr, Dallas, TX, Tel Hashomer, Israel and Haddassah Univ Hosp, Jerusalem, Israel.

There are 8 known variants of DR4-DRBI which associate with DQw7 or DQw8 in Caucasians and with DQw4 in Orientals. Little is known about the distribution of these variants or their DQ associations in other ethnic groups. In the present study we have analyzed 59 N.A. Caucasians, 44 Israeli Jews and 25 Chinese from North China, all having DR4. DR4-DRBI genes were amplified with group-specific primers. Locus-specific primers were used for DQAI and DQBI. The dominant DRBI subtypes were totally different in each race. Dw4 dominated the American Caucasian DR4 panel (55.9%), DwI0 was the major type of DR4 in Israeli Jews (59.1%) while in Chinese we found mainly KT2 (DRBI*0406, 36%) and Dwl5 (DRBI*0405, 32%). Each of these alleles was rare in the other ethnic groups. DQAI*0301 was found in virtually all DR4 haplotypes while associations with DQBI alleles were variable in different panels. In all 17 DRBI- DQBI haplotypes were determined and confirmed by family studies. Several of these were not previously described: DRBI Dw DQ Race DRBI Dw DQ Race 0403 13.1 w4 Jew 0408 14.2 w2 Jew 0404 14.1 w4 Jew 0405 w15 w2 Jew 0401 4 w2 Jew 0405 w15 w8 Cau, Jew These results may be important in transplants and in the interpretation of disease associations in different races.

~142 FOUR-LOCUS HLA CLASS II HAPLOTYPES DEFINED BY PCR AND OLIGONUCLEOTIDE HYBRIDIZATION IN FIVE ETHNIC GROUPS. MA

I0.| Fernandez-Vina, X Gao, ME Moraes, Y Sun, J An, Z Layrisse and P Stastny, UT Southwestern Med Cntr, Dallas, TX, and IVIC, Caracas, Venezuela.

We have analyzed the associations of class II alleles in 480 unrelated individuals including N.A. Caucasoids, Blacks, Latin American, Chinese, and Israeli Jews. Thirty-nine alleles of DRBI, 4 of DRB3, 4 of DRB5, 8 of DQAI, and 14 of DQBI yielded 20 DQAI-DQBI combinations, 65 DRBI-DQAI-DQBI and 71 DRB3/5-DRBI-DQAI-DQBI class II haplotypes, many of which were found only in one ethnic group. The 20 DQAI-DQBI groups and their DR antigens were: DQAI DQBI DR DQAI DQBI DR 0101 0501 l,wl0,wll,wl2 0301 0201 4,7,9

0503 w14 0301 4 0102 0501 wll,wl3,wl5 0302 4,w8

0502 wll,wl3,wl6 0401 4 0602 wll,wl5 0303 9 0604 w13 0401 0402 w8,w18 6.5 w13 0501 0201 w17

0103 0601 w8,w15 0301 l,w8,wll,wl2,wl3, 0603 wlI,wI3 Wl4,Wl6,JX6

0201 0201 7,w13 0601 0301 w8,w12 0303 7 Conclusions: a) there are multiple

sites for recombination in the D region; b) these sites are characteristic for haplotypic combinations; c) maintenance of DQAI-DQBI associations could be due to selective advantage or difficulty in pairing with other alleles.