GlucoseGlucose

Glutamine

Glucose-6P Glucose-6P

Glycerol-3P Fatty Acids

Glutamate

Ribose-5P

2 NADP+

NADP+

NADP+

Acetyl-CoA

α-KG

ETC

Malate

Fructose-6P

Fructose-1,6-P2

1,3-Biphosphoglycerate

3-Phosphoglycerate

2-Phosphoglycerate

Phosphoenolpyruvate

Pyruvate

Pyruvate

Glyceraldehyde-3P Dihydroxyacetone-P

PI3K

PI3K

PI3K

MYC

MYC

MYC

NUCLEOTIDESYNTHESIS

NUCLEOTIDESYNTHESIS

LIPIDSYNTHESIS

HIF

p53p53

Lactate

p53

PAMPK

AMPK

2 NADPH

2 NADPH

2 NADPH

HIF

HIF-1

p53

HIF

HIF

HIF

HIF

MYC

HIFAldolase

Glutaminase

GLUT1

HexokinaseHexokinase 2

Pyruvate Kinase

Phosphofructokinase

Phosphoglucomutase

Enolase

Pyruvate Kinase

PhosphoglucoseIsomerase

PhosphoglycerateKinase

Triosephosphate Isomerase

Fatty Acid Synthase

ATP Citrate Lyase

PyruvateDehydrogenase

Lactate Dehydrogenase A

1 3 B

Glyceraldehyde-3-phosphate Dehydrogenase

Pyruvate Dehydrogenase Kinase 1

Carnitine Palmitoy-ltransferase 1

Glucose Transporter 1

ROS

Acetyl-CoA

Acetyl-CoA

α-Ketoglutarate

FATTY ACIDSYNTHESIS

ElectronTransport

ChainMYC

MYC

MYC

HIF

HIF

HIF

Aconitase 2

CitrateSynthase

PyruvateDehydrogenase

α-KetoglutarateDehydrogenase

LactateDehydrogenase

Fatty Acid Synthase

Pyruvate Dehydrogenase Kinase 1

C AA

Carnitine Palmitoy-ltransferase 1

ROS

LIPIDSYNTHESIS

LIPIDSYNTHESIS

Pyruvate

Oxaloacetate Citrate

Isocitrate

Glutamate

Glutamine

α-Ketoglutarate

Dehydry ogeg na

Succinyl-CoA

Succinate

CitratePyruvateLactate

Glutaminase

2HGMalate

Fumarate

Malate

Succinyl-CoA Ligase

Malic Enzyme 1

eMalate

Dehydrogenase

Mutant FumarateHydratase

Mutant IsocitrateDehydrogenase 2

Mutant IsocitrateDehydrogenase 1

Mutant SuccinateDehydrogenase

TCA Cycle

Citrate

at abcam.com/cancer

Copyright © 2014 Abcam, All Rights Reserved. The Abcam logo is a registered trademark. All information / detail is correct at time of going to print.Produced in collaboration with Meghan Verschoor (McMaster University, Hamilton, Ontario, Canada).

Kits Size Description Species Product reactivity code

MitoProfile® Total OXPHOS Human 360 or 720 µg The cocktail is suitable for Western Blotting Hu ab110411WB Antibody Cocktail analysis of the relative levels of the 5 OXPHOS

complexes in mitochondrial preparations

MitoProfile® Total OXPHOS Rodent 360 or 720 µg The cocktail is suitable for Western Blotting Ms, Rt ,Hu, ab110413 WB Antibody Cocktail analysis of the relative levels of the 5 OXPHOS Cow, Mk

complexes in mitochondrial preparations

ATP citrate lyase (ab40793)

Clonality Applications Host Species cross reactivity M WB, IP, ELISA, Rb Hu, Ms, Rt IHC-P, ICC/IF

c-Myc (ab32072)

Clonality Applications Host Species cross reactivity M WB, IP, IHC-P, ICC/IF Rb Hu, Ms, Rt

Glucose Transporter GLUT1 (ab115730)

Clonality Applications Host Species cross reactivity M WB, IHC-P, Flow Cyt, Rb Hu, Ms, Rt ICC/IF

p53 (ab26)

Clonality Applications Host Species cross reactivity M WB, IP, IHC-Fr, IHC-P, Ms Hs, Ms, Rt, Chk, Cow, Flow Cyt, ICC/IF CMk, Dog, Hm, Dog, Hm

Pyrovate Dehydrogenase E2 (ab66511)

Clonality Applications Host Species cross reactivity P ELISA, IHC-P, WB Rb Hu, Ms, Rt, Dog

Aconitase 2 [6F12BD9] M Flow Cyt, ICC/IF, In-Cell ELISA, IP, WB Ms Hu, Rt, Dm ab110321AMPK alpha 1 + AMPK alpha 2 M WB, IP, ELISA, IHC-Fr, ICC/IF Ms Hu, Rt, Dm ab80039c-Myc [9E10] - ChIP Grade ChIP, ELISA, Flow Cyt, ICC, ICC/IF, IHC (Methanol fixed), IHC-Fr, IHC-P, M IP, Purification, WB Ms Hu, Ms, Dm ab32Fatty Acid Synthase P ICC/IF, IHC (PFA fixed), IHC-Fr, IP, WB Rb Hu, Ms, Rt ab22759Glucose Transporter GLUT1 P WB, IP, IHC-P, ICC/IF Rb Hu, Ms, Rt ab32551Hexokinase II P ELISA, IHC-P, WB Rb Hu ab37593HIF1 alpha M WB, IP, IHC-P, Flow Cyt, ICC/IF Rb Hu ab51608Lactate Dehydrogenase M WB, IP, IHC-P, Flow Cyt, ICC/IF Rb Hs, Ms, Rt ab52488Mitochondrial Pyruvate dehydrogenase kinase 1 P WB, IP, IHC-P, ICC/IF Rb Hu, Ms, Rt ab92959p53 WB, IP, ELISA, IHC-Fr, IHC-P, M Flow Cyt, ICC/IF Ms Hu, Rt, ab28PFKL M WB, Flow Cyt, ICC/IF Rb Hs, Ms, Rt ab181064Pyruvate Dehydrogenase E2 Hu, Ms, M WB, IHC-P, Flow Cyt, ICC/IF Ms Rt, Cow ab110333GAPDH P WB, IP, ELISA, IHC-Fr, Flow Cyt, ICC/IF Rb Hs, Ms, Rt, Ckn, ab9485 AGMk, XI, Sc, Sp

And for superb IF and ICC results use our pre-adsorbed secondary antibodiesAvailable conjugated to: Alexa Fluor® 405, Alexa Fluor® 488, Alexa Fluor® 555, Alexa Fluor® 568,

Alexa Fluor® 594, Alexa Fluor® 647, Alexa Fluor® 750

Key

MitoScience Rat - Rt Chicken - Chk Saccharomyces cerevisiae - ScRabMAb Cow-Cow Xenopus laevis - XI Saccharomyces pombe - SpHuman - Hu Dog - Dog Syrian hamster - Hm Drosophila melanogaster - Dm Mouse - Ms Monkey - Mk Cynomolgus Monkey - CMk African Green Monkey - AGMk

Antibody Clonality Applications Host Species Product reactivity code

DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Biochemicals Size Description Product code Diclofenac Sodium 1g Potent cyclo-oxygenase inhibitor ab120621FCCP 10mg,50mg Mitochondrial oxidative phosphorylation inhibitor ab120081Lovastatin 10mg,50mg Potent HMG CoA reductase inhibitor ab120614NS 398 10mg,50mg COX-2 inhibitor ab120295

Product highlights