GEORGE A. ZACHARIADIS ASSOCIATE PROFESSOR

LAB. OF ANALYTICAL CHEMISTRY, DEPARTMENT OF CHEMISTRY ARISTOTLE UNIVERSITY OF THESSALONIKI, GREECE

CURRICULUM VITAE FOR THE POSITION OF

“PROFESSOR” OF CHEMISTRY DEPARTMENT, AUTH

IN

ANALYTICAL CHEMISTRY

PARTS I AND II

INCLUDING BIOGRAPHIC DATA, STUDIES, TITLES, TEAACHING, ADMINISTRATIVE AND RESEARCH ACTIVITIES

PUBLISHED WORKS AND BOOKS

THESSALONIKI NOVEMBER 2012

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CONTENTS

PART Ι BIOGRAPHY, SCIENTIFIC, EDUCATIONAL, ADMINISTRATIVE WORK No Title of section Page

1 SUMMARIZED CURRICULUM VITAE ………………………………..………………………… 3

2 HIGHLIGHTS AND RECOGNITION OF SCIENTIFIC WORK ………………………………….. 5

3 ACADEMIC POSITIONS …….………………………………….…….…………………….…. . 6

4 ADMINISTRATIVE DUTIES .……………………………………………………………………..… 6

5 MEMBER OF CONFERENCE ORGANIZATION COMMITTEES …………………………….. 7

6 EDUCATIONAL DUTIES IN UNIVERSITY ……………………………………………………... 7

7 INVITATIONS FOR LECTURES IN SEMINARS AND CONFERENCES ……………………… 8

8 SCIENTIFIC RESEARCH AREAS ………………................................................................... 8

9 SUPERVISOR AND MEMBER OF ADVISORY COMMITEES IN Ph.D. THESES …..….. 9

10 SUPERVISOR OF POST DOCTORAL RESEARCHERS ………………………………..…... 9

11 SUPERVISOR OF POST GRADUATE MASTER STUDENTS ………………… ………..…. 9

12 SUPERVISOR IN SENIOR THESES OF UNDERGRADUATE STUDENTS ……………….. 10

13 PARTICIPATION IN INTERNATIONAL INTERLABORATORY TESTS ……………. ……. 10

14 COOPERATIONS WITH OTHER LABORATORIES AND INSTITUTES …………………….. 11

15 EDITORIAL BOARDS AND PAPER REVIEWING ……………………………………………… 12

16 PARTICIPATION IN RESEARCH PROJECTS ………….…………………………………..… 13

17 PROPOSALS OF RESEARCH PROJECTS …………………………….…………….……..… 14

18 PARICIPATION IN CONFERENCES …………………………………….……………………….. 15

19 PARTICIPATION IN SCIENTIFIC SEMINARS ……….……………….……………………….. 17

20 INTERNATIONAL BOOK ………………………………………………………………..… 17

21 TUTORIAL BOOKS ………………..………………………………………………………..…. . 18

22 MY DOCTORAL THESIS ………………………………………………………………..… 19

23 PUBLISHED PAPERS IN PEER REVIEWED JOURNALS………………………………………. 19

24 CONFERENCE ANOUNCEMENTS ….…………………………………………………………..… 27

25 ARTICLES PUBLISHED IN PERIODICALS ……………………………………………..… 37

26 IMPACT FACTORS OF JOURNALS ………………………………… …………………………. 38

27 BIBLIOGRAPHIC STATISTICS FOR MY PAPERS …………………………………………….. 41

PART ΙΙ ANALYSIS OF SCIENTIFIC WORK ………………………………….. 42

PART ΙΙΙ PAPERS IN INTERNATIONAL PEER REVIEWED JOURNALS ….. 76

PART ΙV CITATIONS IN MY PAPERS …………………………………………… 76

PART V ANOUNCEMENTS IN CONFERENCES ……………………………… 76

PART VΙ MY DOCTORAL THESIS BOOK……………………………………… 76

PART VΙΙ INTERNATIONAL BOOK FOR ICP-AES TECHNIQUE ………….… 76

PART VΙΙI COPIES OF MY TUTORIAL BOOKS ……………………………… 76

PART ΙX FORMAL DOCUMENTS………………………………………………… 76

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PART Ι BIOGRAPHY, SCIENTIFIC, EDUCATIONAL, ADMINISTRATIVE WORK

1. SUMMARIZED CURRICULUM VITAE

Name: George Zachariadis Website: http://users.auth.gr/~zacharia/ e-mail: zacharia@chem.auth.gr Telephone: (0030) 2310 997707 Profession: Associate Professor, Lab. of Analytical Chemistry, Dept. of Chemistry,

Aristotle University of Thessaloniki, AUTH. GREECE Birth date, place: 1963, Thessaloniki, Greece Undergraduate Studies: Bachelor in Chemistry, (1986), Aristotle University Thessaloniki Postgraduate Studies: Ph.D. Thesis (1991), Dept. of Chemistry, Aristotle University of

Thessaloniki, Greece, title: Determination of Mercury Compounds by Cold Vapor Atomic Absorption Spectrometry and Factorial Design.

Post Doctoral Studies: Technical University of Vienna, Austria, (2006, 2009) Scholarships: Undergraduate Scholarship, National Scholarship Foundation (1982-83)

Postgraduate Scholarship, National Scholarship Foundation (1988-90) Teaching duties: Analytical Chemistry, (Quantitative, Instrumental and Advanced Analytical

Chemistry) for under- and postgraduate students of Chemistry, Pharmaceutics, Biology etc. between the years 1997 to 2012.

Seminars: Attendance in 12 Scientific Seminars on Instrumental techniques of

analysis Teaching courses of Analytical and Environmental Analytical Chemistry and Chemometrics in various seminars for post graduate students and professionals.

Member of Associations: International Association of Environmental Analytical Chemistry,

European Virtual Institute on Speciation Analysis, Greek Chemical Association, North Greece Chemists‘ Society, Greek Archaeometric Society.

Conferences’ Committees: Co-Chairman in Flow Analysis 2012 International Conference, Member of

organizing and Scientific Committees in 4 other International Conferences (IMA 2003, AACD 2006, AACD 2010, IMA 2011).

Administrative work: Member of the general Assembly of Department of Chemistry Member of the Committees for the Undergraduate Curriculum, DChem. Member of the Committees for the Postgraduate Curriculum Research fields: Atomic spectrometry; Mass spectrometry; Hyphenated techniques;

Multielement methods of analysis; Speciation; Sample pretreatment; Chemometrics; More precisely, the current research is focused on the development of (i) speciation methods using hyphenated techniques like GC-MS, GC-MIP-AED, LC-MS, etc. (ii) off-line analytical methods for multi-element analysis of complex matrices like foods (honey, sugars, cereals, yeasts etc) and silicates (ceramics, glasses etc.) and (iii) on-line analytical methods for preconcentration and speciation of heavy metals and their determination in biological and environmental matrices.

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Supervisor: 4 Doctoral Students, 1 Post Doc, 12 Master students, 18 Senior theses st Co-Supervisor: 1 Doctoral student and 2 Master students. Book authoring: 1 International book in ICPAES technique under the title ―Inductively

Coupled Plasma Atomic Emission Spectrometry, A model multi-element technique for modern laboratory‖, NOVA Science Publishing, New York, 2012. 11 Books in Analytical Chemistry. Tutorial books in greek, for students of Chemistry, Pharmaceutics, Biology and Geology. Course books in Analytical Chemistry (introductory) (1996 and 1998), Quantitative Analytical Chemistry (1999 and 2011), Instrumental methods of Analysis (2004), Special methods of Separation and Chemical Analysis (2005).

Editor: Editorial Board in American Journal of Analytical Chemistry

Guest Editor in Current Analytical Chemistry, Special Issue on ―Metals Speciation and Metallomics‖. Reviewer in 30 International Peer-Review Journals, for 86 papers.

Projects: 35 research projects. 4 research projects as Main Researcher and Supervisor Conference Presentations: 111 announcements in International and National Conferences. Publications in Journals: 107 original research and review papers in International Peer-reviewed

journals (See the list of publications below). Citations: 1636 (November 1, 2012, excluding self ciations) H-index: H-Index = 23 G-index: G-index = 37

Summary of Research and Authoring Work:

Papers in journals: 107 papers in international peer reviewed journals (Γ1 to Γ107)

Γ90, Γ91 are Reviews, Γ106 is Editorial.

Papers as Associate Prof: 32 papers ( after Γ76)

Corresponding author: 37 papers as Associate or Assistant professor

Conference Anouncement: 111 presentations in internat. and national conferences (Α1 to Α111)

Present. as Associate Prof: 35 presentations as Associate professor (Α77 to Α111)

Participations in Conference: 39 participations (1 to 39)

Participations in Seminars: 12 scientific seminars (Μ1 to Μ12)

Research projects: 34 projects. In 4 as Supervisor (Π1-Π30, Τ1-Τ4)

Other articles in journals: 2 scientific articles in regular journal (Σ1 and Σ2).

Ph.D. Supervising: In 4 as Supervisor and in 1 as co-supervisor.

Postgraduate diploma work: 12 as Supervisor, 2 as co-supervisor.

Highlights: 1 papers in 25 most downloaded papers of ACA.

2 Distinction Prizes Scholarships for my Ph.D. students.

International book: 1 monography on ICP-AES, NOVA SCIENCE, New York (Ν1).

Greek books: 11 tutorial books, teached in Univeristy (Β1 to Β11). B12 is in edit.

Course curriculum: I was involved in the formation of 3 courses in Undergraduate

Chemistry Curriculum and of 2 courses in Post Graduate Chemistry.

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Summary of Teaching duties:

Courses: ARISTOTLE UNIVERSITY OF THESSALONIKI

Undergraduate Level: Teaching courses of Analytical Chermistry and

instrumental Methods of Analysis in Students of Chemistry,

Pharmaceutics, (1998-2013) Biology, (1998-2001)

Postgraduate Level: Courses in 4 Post-Graduate Curriculae:

Α) Advanced Chemical Analysis,

Β) Chemical Analysis and Quality Control,

Γ) Conservation and Restoration of Art Objects.

Γ) Nanoscience and Nanotechnology.

TECHNICAL UNIVERSITY OF WIEN:

Postgraduate Level: Teaching in Seminars of Institut fur Chemische

Technologien und Analytic (after 2004).

ΗΔΚ: Teaching General Chemistry in the curriculum of "Techniciens

for Pharmaceuticals and Cosmetics" (1993-1995).

In High School : I teached Chemistry for University candidates (1987-

1990).

Laboratory excercises: Laboratory excercises in almost all courses of Analytical Chemistry

(1988 - 2011). Responsible for the organization of Laboratory of

Analytical Chemistry Pronciples (2000 - 2004).

Seminars Courses on "Sampling methods, Chemical Analysis. Chemometrics.

In Environemntal Protection Seminars (Kavala 1989, Katerini 1992,

Veria 1993). "Instrumental Methods of Chemical Analysis" (1999).

Member of Scientific Associations and Societies:

International: International Association of Environmental Analytical Chemistry (S1)

European Virtual Institute of Speciation Analysis. (S2)

National: Associations of Greek Chemists and of North Greece Chemists

Greek Society of Toxicology (S3)

Greek Archaeometry Society (S4)

2. HIGHLIGHTS AND RECOGNITION OF SCIENTIFIC WORK

1. 1636 Citations excluding self citations in my papers in scientific journals (November 1, 2012).

2. Invitation as Author of one International Book about ICP-AES, 2012.

3. Invitation as Guest Editor in Special issue in Current Analytical Chemistry, 2012.

4. Invitation as Reviewer of 86 submitted papers in 30 interantional journals of Chemistry.

5. One paper in Αnalytica Chimica Acta in the top 25 most downloaded in year 2008.

6. The conclusions of my paper in Sunscreens were included in the article of Wikipedia.

7. Invitation to give 8 Lectures/presentations

8. Two Ph.D. students received Excellence Scholarship for their thesis research.

9. Application from researchers for post doc supervising.

10. Involvement in joint proposals in European Union on Speciation and on Biometals.

11. Appreciation of my research on the presence of metals in pharmaceuticals from the chair of

the Advisory Committee of Unite States Pharmacop., Dr. Nancy Lewen

12. Appreciation of my research on analytical methods for organometallic analytes from the

Chairman of the Chemische Technologichen Institute of TU Wien Herbert Danniger.

13. Three of my tutorial books were teached in other greek universities.

14. Invitation for articles in Journal of Chromatography A, and Analytica Chimica Acta.

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3. ACADEMIC POSITIONS

24/7/2008 – Up today: Associate Professor, Lab. of Analytical Chemistry, Dept. of

Chemistry (12-5-2008). 14/9/2006 – 24/7/2008: Assistant Professor (Permanent position), Lab. of Analytical

Chemistry, Dept. of Chemistry 10-4-2006. 18/2/2003 – 14/9/2006: Assistant Professor, Lab. of Analytical Chemistry, Dept. of Chemistry

10-4-2006. 1/7/1998 - 25/11/2002: Lecturer, Lab. of Analytical Chemistry, Dept. of Chemistry 10-4-2006. 5/11/1990 - 30/6/1998: Special Teaching and Technical Assistant position, Dept. of

Chemistry. 1/3/1988 - 5/11/1990: Special Post-graduate, Assisting Stuff with Scholarship, Dept. of

Chemistry, ΑΠΘ. Other professional exper.: Assistant Chemist, in Industry (1985).

4. ADMINISTRATIVE DUTIES

1998 - 2001: Member of the General Assembly of Chemistry Department, AUTH. 1999 - 2000: Represenative of Chemistry Department in AUTH Senate. 1999 - 2001: Member of the Committee for the Reformation of Undergraduate Curriculum

of Chemistry Department, AUTH, which was finally accepted from dept.. 2002 - 2003: Member of the Proposal Submitting Committee for the foundation of the

reformation of the undergraduate program of Chemistry Department, AUTH. The proposal was accepted and founded in 2004.

2002 - 2003: Member of the Proposal Submitting Committee for the participation of the Laboratory of Analytical Chemistry in the Ministerial network of Academic Laboratories for the national Monitoring of Surface Waters of Ministry of Environment.

2004 - 2005: Member of the General Assembly of Chemistry Department, AUTH 2004 - 2005: Member of the Committee for the Undergraduate Curriculum of Chemistry

Department, AUTH. 2005 - 2006: Member of the General Assembly of Chemistry Department, AUTH 2005 - 2006: Member of the Joined Committee for the Undergraduate and Postgraduate

Curriculum of Chemistry Department, AUTH to obtain EUROBACHELOR. 2008 - 2009: Member of the Committee for the Postgraduate Curriculum of Chemistry

Department, AUTH. 2009 - 2010: Member of the General Assembly of Chemistry Department, AUTH 2009 - 2010: Member of the Committee for the Undergraduate Curriculum of Chemistry

Department, AUTH. 2010 - 2011: Member of the Students‘ Advisory Committee of Chemistry Department. 2008 - 2012: Member of the Control Committee of Instrumentation in Analytical Chemistry

laboratory, Dept. of Chemistry Department, AUTH 2006 - 2012: Member of the Library Committee of Chemistry Department, AUTH I was member in 6 recommendation committees in relation to Analytical Chemistry research field for the following academic development procedures: 1. Development to Assistant Professor, Dept. of Chemistry, AUTH (2006).

2. Development to Assistant Professor, Dept. of Chemistry, AUTH (2006).

3. Development to Assistant Professor, Dept. of Chemistry, Athens Univ, (2006).

4. Development to Associate Professor, Dept. of Chemistry, AUTH (2006).

5. Development to Assistant Professor, Dept. of Chemistry, Patras Univ, (2011).

6. Election to Lecturer, Dept. of Chemistry, Patras Univ, (2011).

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5. MEMBER OF CONFERENCE ORGANIZATION COMMITTEES

1. Organizing Committee of Internat. Confer. on Instrumental Methods of Analysis, IMA 2003" 2. Organizing Committee of Intern. Confer. "5th Aegean Analytical Chemistry Days, AACD 2006". 3. Scientific Committee of Intern Conference "7th Aegean Analytical Chemistry Days, AACD 2010. 4. Scientific Committee of Intern. Conference on "7th Instrumental Methods of Analysis, IMA 2011. 5. Co-Chairman of Intern. Conference on ―Flow Analysis XII, FA 2012‖ .

6. EDUCATIONAL DUTIES IN UNIVERSITY

Teaching courses: UNDERGRADUATE LEVEL: 1998 - 2013: Principles of Analytical Chemistry, 1ο Semester, Dept. of Chemistry. 2010 - 2013: Quantitative Analytical Chemistry, 2ο Semester, Dept. of Chemistry. 1998 - 2008: Quantitative Analytical Chemistry, 2ο Semester, Dept. of Pharmaceutics. 2008 - 2013: Analytical Chemistry, 1ο Semester, Dept. of Pharmaceutics. 2003 - 2013: Instrumental Chemical Analysis (II), 7ο Semester, Dept. of Chemistry. 2003 - 2013: Special Methods of Separation and Chemical Analysis, 8ο Semester, Dept.Chem.

2005 - 2013: Archaeometry, 8ο Semester, Dept. of Chemistry.

1998 - 2003: Spectroscopic methods of Analysis, 7ο Semester, Dept. of Chemistry. 1998 - 2003: Advanced Quantitative Chemical Analysis, 7ο Semester, Dept. of Chemistry. POSTGRADUATE LEVEL: 1998 - 2013: Advanced Instrumental Analysis, 1ο Semester, in Chemistry postgraduate program. 2002 - 2013: Chemometrics-Statistics-Metrology, 1ο Semester, in ―Chemical Analysis and Quality

Control‖ postgraduate program. 2004 - 2013: Atomic Spectrometry, 2ο Semester, in ―Conservation and Restoration of Art

Objects‖ postgraduate program. 2010 - 2013: Analytical, measurement and control techniques, 3ο Semester, in ―Nanoscience

and Nanotechnology‖ postgraduate program. Laboratory excercises for Undergraduate Students 1988 – 1999 Instrumental Chemical Analysis (Dept. of Chemistry) 1989 – 1992 Quantitative Chemical Analysis (Dept. of Chemistry) 1989 – 1998 Analytical Chemistry (Dept. of Biology and Dept. Agriculture) 1992 – 1999 Qualitative Chemical Analysis (Dept. of Chemistry) 1995 – 1996 Analytical Chemistry (Depts. of Geology) 1997 – 2003 Spectroscopic methods of Analysis (Dept. of Chemistry) 1997 – 2000 Advanced Quantitative Analysis (Dept. of Chemistry) 1999 – 2008 Quantitative Analytical Chemistry (Dept. of Pharmaceutics) 2008 – 2010 Analytical Chemistry (Dept. of Pharmaceutics) 2000 – 2010 Principles of Analytical Chemistry (Dept. of Chemistry) 2003 – 2013 Instrumental Chemical Analysis II (Dept. of Chemistry) 2003 – 2013 Special Methods for Separation and Chemical Analysis (Dept. of Chemistry)

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7. INVITATIONS FOR LECTURES IN SEMINARS AND CONFERENCES

I was Invited to give lectures in the following Conferences and Seminars. Documents of the relevant correspondence are given in the CD, Part IX, Chapter 7. Υ-1. (2004) In Technische Universitat Wien, Austria, Institute fur Chemische Technologie und Analytik, with the title «Hyphenation of On-line Solid Phase Extraction with Atomic Spectrometric Techniques. A Convenient Tool to Enhance the Analytical Performance of Mercury Determination and Speciation». Υ-2. (2006) In Technische Universitat Wien, Austria, Institute fur Chemische Technologie und Analytik, with the title ―Inductively Coupled Plasma Atomic Emission Spectrometry with various sample introduction techniques»” Υ-3. (2011) In Technische Universitat Wien, Austria, Institute fur Chemische Technologie und Analytik, with the title «Plasma Emission Spectrometry and its applications». Υ-4. (2009) In EEESD 09 International Conference in Athens, Greece, on «Modern hyphenated analytical techniques for the analysis of environmental, food, and biological matrices» Υ-5. (2011) In BIOMEDCH11 International Conference in Cambridge, United Kingdom, with the title «Recent developments in high Resolution tandem mass spectrometry for analysis of biomolecules and related analytes». Υ-6. (2007) In seminar of Medical School of Larissa, Greece, with the title ―Atomic Absorption Spectroscopy and its Applications in Environemntal Chemistry and Toxicology‖. Υ-7. (2007) In seminar of Medical School of Larissa, Greece with the title ―Atomic Spectroscopy and its Applications‖ Υ-8. (2012) In seminar of Polytechnic School, Aristotle University, Thessaloniki, on Diagnostic Techniques in Science of Conservation of Art Objects‖ with the title ―Modern Analytical Techniques based on Spectrometry and Plasma Atomization‖

8. SCIENTIFIC RESEARCH AREAS

Hyphenated Techniques and Mass Spectrometry: Development and evaluation of hyphenated analytical methods using spectrometric detectors. Gas chromatography with microwave induced plasma atomic emiision spectrometry (GC-AED) or with mass spectrometry (GC-MS). Development of Metal Speciation methods of analysis of biological samples and Metallomics research for Bioanalysis, using modern hyphenated techniques like liquid chromatography mass spectrometry (LC-MS) or liquid chromatography plasma emission spectrometry. Atomic Spectrometry Techniques: Development and evaluation of analytical methods using Inductively Coupled Plasma Atomic Emission Spectrometry (ICPAES), Atomic Absorption Spectrometry with Flame (FAAS), or Graphite Furnace (ΔΣAAS), or Hydride Generation and Cold Vapor Generation, (HGAAS and CVAAS) either in batch mode or by on-line coupling with other and automated flow analysis techniques. Also development of non-destructive methods based on X-ray fluoresence spectrometry. Speciation analysis: Development of analytical methods for speciation analysis of organometallic species in biological matrices using separation techniques and atomic or mass spectrometric detectors. Methods for the analysis of archaeometric specimens, pharmaceutical matrices, soils and sediments, plant and animal tissues and organisms, biological fluids, food and food supplements, natural waters, silicates, alloys, standards, etc. Sample pretreatment techniques: Development of wert-acid digestion methods, microwave assisted digestion, liquid-liquid extraction, solid phase extraction, headspace solid phase microextraction, etc. Chemometrics: Analysis of Variance, Regression Analysis, Cluster Analysis, Principal Component Analysis, Factorial Designs, Experimental Design, in Analytical methods optimization, evaluation and data interpretation.

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9. SUPERVISOR AND MEMBER OF ADVISORY COMMITTEES IN Ph.D THESES

1. Supervisor in thesis of Christos Vogiatzis in "Methods of Speciation analysis of metals and trace elements and metallomic studies by hyphenated mass spectrometric and plasma spectrometric techniques". Started May 2011. 2. Supervisor in thesis of Nikolaos Tzollas in "Development and optimization of speciation methods for tin species by hyphenated techniques with chromatography mass spectrometry and atomic spectrometry". Started March 2008. To be defended early in 2013. Gained Prize of Distinction during 2011. Three published papers until now. 3. Supervisor in thesis of Dimitra Kapsimali in "Determination of organometallic selenium compounds in biological and food matrices by techniques with extraction and chromatography hyphenated to mass spectrometry". Started June 2008. Completed September 2012. Gained Prize of Distinction during 2011. Four published papers. 4. Supervisor in thesis of Emmanouel Daftsis in "Development of methods for determination of metals ions and compounds with biomolecules by hyphenated atomic spectrometric techniques and chemometric optimization". Started June 2003. Completed Sept 2007 3 published papers. 5. Co-supervisor in thesis of Awwad momen in "Development of multielement analytical methods for food analysis by inductively coupled plasma atomic emission spectromety". Started June 2002. Completed September 2006. Four published papers. 6. I was also member of the Advisory Committee in 6 other Doctoral Theses

10. SUPERVISOR OF POSTDOCTORAL RESEARCHERS

1. Post Doc research of Lemonia Valianou in the field of the ―Development of analytical methods for the analysis of archaeological samples towards the investigation of the use of pharmaceuticals and cosmetics in ancient times‖. January 2011 until January 2012. 2. Application of Αwad Momen (From Yuba university, Sudan) for Postdoc research in the ―Development of Multivariable Chemometrical Methods for experimental design and optimization of Plasma Atomic Emission Spectrometry. I accepted but not started yet until beginning funding from Yuba University. 3. Applications from several other students for supervising their doctoral thesis.

11. SUPERVISOR OF POSTGRADUATE (MASTER) STUDENTS

1. Supervisor in master of Evangellos Trikas in "Speciation of Germanium by hyphenated gas chromatography microwave plasma spectrometric technique". (2012-2013). 2. Supervisor in master of Anna Katsavou in "Development of analytical method for platinum determination in human tissues by inductively coupled plasma atomic emission spectrometry". (2012-2013). 3. Supervisor in master of Efrosini Sahanidou in "Archaoemtric study of organic residues by solid phase microextraction and gas chromatography mass spectrometry". (2012-2013). 4. Supervisor in master of Eleni Spanou in "Determination of metal and non-metal anions by ion chromatography and inductively coupled plasma atomic emission spectrometry". (2011-2012). 5. Supervisor in master of Chrysanthi Karageorgaki in "Study on preconcentration and determination of platinum anticancer drugs in biological fluids by inductively coupled plasma atomic emission spectrometry ". (2011-2012). 6. Supervisor in master of Anastasia Lyratzi in "Determination of alkali and alkaline earth cations in drinking water, pharmaceutical preparations, and energy drinks by high pressure ion chromatography". (2010-2011). 7. Supervisor in master of Christos Vogiatzis in "Investigation of the use of yttrium as internal standard in aqueous and non-aqueous solutions in inductively coupled plasma atomic emission spectrometry ". (2009-2010). 8. Supervisor in master of Athanasia Laggioli in "Study on the matrix effect and the type of sample pretreatment techniques in the determination of volatile aromatic components of essential oils from mastic products by GC-MS". (2009-2010). 9. Supervisor in master of Antonios Ntantasios in "Development of analytical method for determination of volatile substances in essential oils and plants after extraction and by GC-MS". (2009-2010).

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10. Supervisor in master of Dimitra Kapsimali in "Development of speciation methods for the determination of organomercury species by GC-MS". (2007-2008). 11. Supervisor in master of Lemonia Valianou in "Development of multielement methods for milk analysis by inductively coupled plasma atomic emission spectrometry and slurry introduction". (2005-2006). 12. Supervisor in master of Agathi Olympiou in "Development of multielement methods for the analysis of multivitamin preparations by inductively coupled plasma atomic emission spectrometry and slurry inntroduction". (2005-2006). 13. Co-Supervisor in master of Maria Ioannidou in "Determination of heavy metals in honey and sugars by inductively coupled plasma atomic emission spectrometry and slurry inntroduction". (2003-2004). 14. Co-Supervisor in master of Evangelia Dimitrakoudi in "Development of glass decomposition methods by microwaves and determination of metals by inductively coupled plasma atomic emission spectrometry and slurry inntroduction". (2003-2004).

12. SUPERVISOR IN SENIOR THESES OF UNDERGRADUATE STUDENTS

I was supervisor or (co-supervisor before 2000) in the following senior thesis: (1) Lada Ν. (Ga, FAAS) 1991. (2) Baila A. (Ga, FAAS) 1991. (3) Petridis S. (Polymer decomposition) 1993, (4) Pappi R. (Ashing of polymers and analysis, FAAS) 1994. (5) Sioros V. (olive oil analysis, ETAAS) 1994. (6) Raidou E. (yeast analysis, FAAS) 1997. (7) Siakavaras D. (heavy metlas in sediments) 1997. (8) Drakos A (Cluster Analysis, FAAS) 1999. (9) Mpakali A. (Glass multielement analysis, ICP-AES) 2001. (10) Roussou M. (Calibration techniques, ICP-AES) 2002. (11) Fragki E. (Archaeometric analysis of metals ICPAES and FΑAS) 2003. (12) Tsiknas C. (Cd in drinks - ETAAS) 2000. (13) Chalatsi M. (Sn in drinks - ETAAS) 2000. (14) Kougkoulis I. (Cr - On-line SPE-FAAS) 2001. (15) Bettas P. (Pb - On-line SPE-FAAS) 2001. (16) Sfetsiou E. (Ga - On-line SPE-FAAS) 2002. (17) Karpouzi M. (Hg - On-line SPE - CVAAS) 2003. (18) Farastelis C. (Cd - On-line LLE - ΑAS) 2003. (19) Michos C. (Hg – On-line SPE CV ICP AES) 2004. (20) Tzollas N. (Standard addition in CRMs, ICP-AES) 2005 (21) Kapsimali D. (Direct sample introduction of syrups, ICPAES) 2006 (22) Sarafidou P. (Direct slurry introduction of plants CRM, ICPAES) 2008 (23) Sahanidou E. (Sunscreen analysis, Ti, Zn, Al , ICPAES) 2008 (24) Spanou Δ. (ICPAES) (Internal Standadization, ICPAES) 2009. (25) Trikas Δ. (ICPAES) (Cr and As speciation, ICPAES) 2010. (26) Nikolaou M. (Stability of organotins GCMS in urine) 2011. (27) Ntontou D. (GCMS) (Stability of organomercury in urine) 2011.

13. PARTICIPATION IN INTERNATIONAL INTERLABORATORY TESTS

(i) During the years 1990-1998 and 2005-2009, I participated in the intercalibration and proficiency testing of ΗΑΔΑ employing and testing the performance of analytical methods on the analysis of plant and animal tissues, sediments, waters etc. (ii) From January 1994 until December 1995 I participated in the Quality Assurance Project, QUASIMEME which was organized from the Μarine Laboratory ηοσ Aberdeen, U.K. due to European Commission on behalf of the lab. of Analytical Chemistry for the methodologies of trace elements analysis.

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14. COOPERATIONS WITH OTHER LABORATORIES AND INSTITUTES

(i) Cooperation with the Laboratory of Botany of Department of Biology regarding toxic metals determination in plant tissues. See papers Γ5, Γ7, Γ16, Γ21, Γ22, Γ28, Γ40, Γ42. (ii) Cooperation with two institutes in Berlin and in Paris (visits during May 1991 and June 1993) respectively regarding the methodology of asbestos fibers determination in drinking and natural waters.See work Α10. (iii) Cooperation with the Laboratory of Toxicology and Forensic analysis of Medical School, regarding toxic metals and drugs residues determination in animal tissues. See papers Γ8, Γ9, Γ10, Γ11 θαη Γ19. (iv) Cooperation with the Laboratory of Analytical Chemistry of Sofia University in Bulgaria in the frame of the Greek-Bulgarian Exchange Project, regarding metal determination and chemometrics. See papers Γ15, Γ24, Γ25, Γ32 θαη Γ33. (v) Cooperation with the Laboratory of Environmental Pollution Control of AUTH regarding the analysis of aqueous environmental samples and wastewaters. See papers Γ13, 14, 35, 36, 43. (vi) Cooperation with the Institute of Chemical Technologies and Analytics of the Vienna Technical Univeristy, (2006), in the frame of Erasmus Project, regarding the development of speciation methods for organometallics and various extraction and microextraction methods for gas chromatography mass spectrometry (GC-MS) or atomic emission spectropmetry (GC-MIP-AED). See papers Γ80 θαη Γ81. (vii) Cooperation with the Laboratory of Forest Treatment of the School of Agriculture, AUTH regarding the analysis of plant samples for environmental monitoring purposes. See paper Γ94. (viii) Cooperation with the Laboratory of Conservation of the Archaeological Museum of Thessaloniki, regarding the analysis of ancient samples and specimens. (ix) Cooperation with the Laboratory of Solid State Physics regarding the analysis of innovative semiconductors and the stoichiometry of boron and other chemical elements in the paterial. See paper Γ102. (x) Cooperation with the Laboratory of Pharmaceutical Analysis, and also with the Laboratory of Biochemistry.of AUTH, for the analysis of protein preparations and the determination of metal traces or impurities or biometals in protein sequences.See papers Γ59 and Γ76. (xi) Cooperation with the Lung Disease Clinic of Medical School of AUTH for the development of analytical methodology to be appolied in the analysis of biological tissues from patients with lung cancer, who are treated with carboplatinum, platinum, cisplatinum and other metallodrugs. See paper Γ98 and an article in the international book N1. (xii) Cooperation with the Institute of Chemical Technologies and Analytics of the Vienna Technical Univeristy, (2009) regarding the development of speciation methods for metallomics and organometallics and hyphenated techniques like Liquid Chromatography Mass Spectrometry (LC-MS) with various ionization techniques like ESI and APCI and also Gas Chromatography Mass Spectrometry (GC-MS). See papers Γ90, Γ100, Γ103, 104 and Γ105. This cooperation is continuous with regular visits and work in the institute in 2010, 2011 and 2012.

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15. EDITORIAL BOARDS AND PAPER REVIEWING

Documents of the relevant correspondence are given in the CD, Part IX, Chapter 15. Α. I was invited as Guest Editor in the Special issue of the journal of CURRENT ANALYTICAL CHEMISTRY, in my topic «METAL SPECIATION AND METALLOMICS‖. The issue was completed and is going to be published as Volume 9, in 2013. It includes also an Editorial article written by me. Β. I am nominated as member of the Editorial Board of the journal: AMERICAL JOURNAL OF ANALYTICAL CHEMISTRY. C. I was invited as reviewer in submitted papers in 30 international peer-reviewed journals in 86 scientific works in total. Αnalytical and Bioanalytical Chemistry: (1) paper.

Αnalytica Chimica Acta: (6) papers.

Analytical Letters: (3) papers.

Analytical Methods: (2) papers.

American Journal of Analytical Chemistry: (6) papers.

Bionalysis: (1) paper.

Bioinorganic Chemistry and Applications: (1) paper.

Canadian Journal of Analytical Science and Spectroscopy: (2) papers.

Central European Chemistry Journal: (4) papers.

Chemosphere: (1) paper.

Current Analytical Chemistry: (1) paper.

Current Organic Chemistry: (1) paper.

Environmental Monitoring and Assesment: (3) papers.

Food Analytical Methods: (2) papers.

Food Chemical Toxicology: (1) paper.

Food Chemistry: (2) papers.

International Journal of Environmental Analytical Chemistry: (6) papers.

Italian Journal of Food Science: (1) paper.

Journal of Analytical Atomic Spectrometry: (1) paper.

Journal of Agricult. And Food Chemistry: (1) paper.

Journal of Food Composition and Analysis: (4) papers.

Journal of Food Quality: (2) papers.

Journal of Liquid Chormatography and Related Techniques: (1) paper.

Journal of Pharmaceutical and Biomedical Analysis: (3) papers.

Journal of Separation Science: (2) papers.

Journal of Serbian Chemical Society: (9) papers.

Microchimica Acta: (4) papers.

Pharmaceutica Analytica Acta: (1) paper.

Talanta: (13) papers.

Toxicological and Environmental Chemistry: (1) paper.

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16. PARTICIPATION IN RESEARCH PROJECTS

I was Scientific Supervisor in the following research projects (Τ1-Τ4), and one of the main researchers in other 30 research projects (Π1 - Π30). Y1 Μέζνδνη πνιπζηνηρεηαθήο αλάιπζεο ζηε κειέηε πιηθώλ. (2008-2009)

ISOMAT Α.Δ. Τ2 Δθαξκνγέο Αλαιπηηθώλ Μεζόδσλ ζηε κειέηε θπζηθνρεκηθώλ παξακέηξσλ

ησλ λεξώλ ηεο πεξηνρήο νρνύ. (2009-2010) Municipality of Sohos. Τ3 Έιεγρνο κεηαβνιώλ θπζηθνρεκηθώλ παξακέηξσλ κε ηελ εθαξκνγή κέηξσλ

βειηίσζεο ηεο πνηόηεηάο ηνπ. (2010-2011) Τ4 Μειέηε κεζόδσλ αλάιπζεο πιώλ θαη ηέθξαο από δηαδηθαζίεο αλαθύθισζεο

αινπκηλίνπ (2012-2013) ΜΔΣΑΜ. Π1 Μειέηε ζαιάζζηαο ξύπαλζεο ζηνλ όξκν ηεο Νέαο Καξβάιεο.

1986-87. (See works Α1 θαη Γ2). Π2 Μειέηε παξακέηξσλ ξύπαλζεο ηνπ πνηακνύ Αμηνύ θαη ηεο ιίκλεο Aγίνπ Βαζηιείνπ.

1987-89. (See works Α5, Α6, Γ3 θαη Γ4). Π3 Δπηζηεκνληθή ζπλεξγαζία ζε ζέκαηα ξύπαλζεο ηνπ Παγαζεηηθνύ θόιπνπ.

1988. (See work Α2). Π4 Μειέηε ηνπ νηθνζπζηήκαηνο ηεο παξάθηηαο πεξηνρήο Ξάλζεο.

1988-89. Η. ηράηες. Π5 Έξεπλα ησλ πεξηβαιινληηθώλ επηπηώζεσλ ζηνλ θόιπν ηεο Θεζζαινλίθεο.

1987-1991. Π6 Μειέηε ηνπ νηθνζπζηήκαηνο ηνπ πνηακνύ 'Δβξνπ.

1991-1992. (See work Γ12). Π7 Αλαιπηηθνί πξνζδηνξηζκνί θαη δηεξγαζίεο θαζαξηζκνύ επηθαλεηαθώλ λεξώλ.

Πεξίπησζε Αιηάθκνλα. 1991-1993. (See works Α10, Γ13, Γ14 θαη Α20). Π8 πλερήο παξαθνινύζεζε ηεο πεξηβαιινληηθήο θαηάζηαζεο ηνπ Θεξκατθνύ θόιπνπ. (MED POL PHASE I) 1991-1994. Π9 Παξαθνινύζεζε πνηόηεηαο απνβιήησλ Θεζζαινλίθεο θαη απνδέθηε Θεξκατθνύ

θόιπνπ ζηελ πεξηνρή Αλαηνιηθνύ Αληιηνζηαζίνπ. 1992-1993. Π10 Παξαιαβή γαιιίνπ από πδαηηθά δηαιύκαηα κε εθρύιηζε.

1993-1994. Π11 Πξνζδηνξηζκόο ηλώλ ακηάληνπ ζε λεξά ηνπ πνηακνύ Αιηάθκνλα θαη ηνπ δηθηύνπ ηεο πόιεο ηεο Θεζζαινλίθεο. 1993-1994. Π12 Έιεγρνο θαη Αμηνιόγεζε ηεο ηιύνο από κνλάδεο βηνινγηθνύ θαζαξηζκνύ αζηηθώλ

ιπκάησλ (ΟΑΘ) θαη βηνκεραληθώλ απνβιήησλ (ΔΣΒΑ), Θεζζαινλίθεο - ίλδνπ. 1994. (See works Α21, Γ26 θαη Γ27).

Π13 Eιεγρνο επηβάξπλζεο ησλ επηθαλεηαθώλ πδάησλ ηνπ πνηακνύ Αμηνύ θαη ηεο ιίκλεο ηνπ Αγίνπ Βαζηιείνπ. 1993-1995. (See works Α24 θαη Α25).

Π14 πλερήο παξαθνινύζεζε ηεο πεξηβαιινληηθήο θαηάζηαζεο ησλ θόιπσλ Θεξκατθνύ, ηξπκνληθνύ θαη Καβάιαο. (MED POL PHASE II) 1995-1997. Π15 Γίθηπν θαη βάζε δεδνκέλσλ ηεο θαηάζηαζεο ηεο αηκόζθαηξαο ηεοΘεζζαινλίθεο.

1996-1997. (See work Α29). Π16 Έιεγρνο ηεο επηβάξπλζεο ησλ εδαθώλ από ησδηνύρν άξγπξν θαηά ηνλ ςεθαζκό

γηα πξνζηαζία από ην ραιάδη. 1996-1997. (See works Α26, Α27, Α28, Γ29). Π17 Έιεγρνο θαη Αλάπηπμε κή ξππνγόλσλ κπαηαξηώλ ιηζησκέλνπ MnO2. (ΔΠΔΣ ΙΙ)

1995-1997. (See work Α23). Π18 Πξνζδηνξηζκόο παξακέηξσλ ξύπαλζεο ζε λεξά πνηακώλ ηεο Βόξεηαο Διιάδαο

1997-1998. (See works Α38, Α40 θαη Γ36). Π19 πλερήο παξαθνινύζεζε ηεο πεξηβαιινληηθήο θαηάζηαζεο ησλ θόιπσλ

Θεξκατθνύ, ηξπκνληθνύ θαη Καβάιαο. (MED POL PHASE II) 1998-2000. (See works Α39, Γ39 θαη Α48).

Π20 Πξνζδηνξηζκόο παξακέηξσλ ξύπαλζεο ζε λεξά πνηακώλ ηεο Βόξεηαο Διιάδαο Β Φάζε 1999-2000. (See work Α49).

Π21 Δληνπηζκόο πεγώλ ξύπαλζεο κε ίλεο ακηάληνπ ζηα λεξά ηνπ πνηακνύ Αιηάθκνλα. 1997-1999.

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Π22 Αλάπηπμε θαη εθαξκνγή αλαιπηηθώλ κεζόδσλ ειέγρνπ πνηόηεηαο πξώησλ πιώλ θαη ρεκηθώλ αληηδξαζηεξίσλ. 1998-2000.

Π23 Δύξεζε ηεο ρεκηθήο ζύζηαζεο ησλ πγξώλ απνβιήησλ ηνπ αηκνειεθηξηθνύ ζηαζκνύ ζην Γήκν Σακπλαίσλ (Αιηβεξίνπ) θαη αξηζηνπνίεζε ηεο δηαδηθαζίαο αδξαλνπνίεζεο ηεο ηιύνο. 2000.

Π24 Έιεγρνο ηεο ζαιάζζηαο ξύπαλζεο ζηνλ Κόιπν ηεο Ιεξηζζνύ (ηξαηόλη). 2000.

Π25 Αλάπηπμε Γηθηύνπ Διιελνβνπιγαξηθήο ζπλεξγαζίαο γηα ηνπο πνηακνύο ηξπκόλα θαη Νέζην. 2000.

Π26 Πξνζδηνξηζκνί ηλώλ ακηάληνπ ζην λεξό ηνπ πνηακνύ Αιηάθκνλα, ζην ζεκείν πδξνδόηεζεο ηεο Θεζζαινλίθεο. 2001-2002.

Π27 Αξραία Κεξακηθή Σερλνινγία - Γξόκνη Κεξακηθήο: Αλάπηπμε ζπζηήκαηνο γηα ηε δηεξεύλεζε ηεο αξραίαο θεξακηθήο. Δθαξκνγή ζηελ θεξακηθή ησλ Αξραίσλ Αβδήξσλ. (ΠΔΝΔΓ) 2002-2005.

Π28 Αμηνπνίεζε ησλ παξαπξντόλησλ βηνκεραλίαο επεμεξγαζίαο θξνύησλ. (ΠABET) 2003-2005.

Π29 Παξαθνινύζεζε Πεξηβάιινληνο ησλ Διιεληθώλ Θαιαζζώλ. (MED POL PHASE III). Δρεσλεηηθό πρόγρακκα σπό ηελ αηγίδα ηες UNEP. 2004-2006.

Π30 Γεηγκαηνιεςίεο, ρεκηθέο αλαιύζεηο κεηαιεπηηθώλ πιηθώλ από ηα κεηαιιεία

Καζζάλδξαο. (2008-2010).

17. PROPOSALS OF RESEARCH PROJECTS

I was member or supervisor of the research teams and also of the scientific teams which submitted the next research proposals. Documents of these can be found in the CD, Part IX, Cp. 17. 2012 FP7 – HEALTH – 2013 - INNOVATION – Investigator – driven treatment trials to combat or prevent metastases in patients with solid cancer”. Member in a proposal submitted under the title ―Aerosolised Cancer Chemotherapy In Lung – Efficacy and Safety (ACCHIL-ES). Jointed proposal of 10 european academic research centers on the development of innovative chemotherapy techniques for lung cancer treatment using platinum containing anticancer preparations and nanotechnology. 2012 ARISTEIA ΙΙ: Participation in a archaeometric proposal submitted from the Department of History and Ethnology of Thrace University. 2011 EUROPEAN SPACE AGENCY: Editing team in a proposal submitted in the frame of European Space Agency announcements for research projects under the title «Development of Multianalyte systems». 2010 THALES ΙΙ: Scientific Supervisor of 5 research teams in a proposal submitted in the field of «Speciation analysis of metals and organometallics, and their complexes with biomolecules in biological and environmental materilas. AUTH-ΣΔΗΘ. 2010 THALES ΙΙ: Participation in a proposal submitted under the title: Holistic approach in am Excavation Challenge (Archaeometry – Multielemental analsyis – Chemometrics). ΗΠΔΣ – AUTH. 2009 PEOPLE MARIE CURIE ACTION: Proposal submitted from 13 european academic laboratories and 8 private scientific institutes. I was participating from Greece side, to the proposal of establishing a Speciation Νetwork under the title ―INTROSPECT‖ INITIAL TRAINING NETWORK FOR TRACE ELEMENT SPECIATION.. 2009 HERACLITUS ΙΙ: Scientific Supervisor in a proposal submitted in the field of «Study of the determination of organometallic compounds of selenium by extraction techniques and gas chromatography coupled to mass spectrometry».

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2009 HERACLITUS II: Scientific Supervisor in a proposal submitted in the field of «Development and Optimization of analytical methods for metal determination by hyphenated chromatographic techniques with mass and atomic spectrometry. 2005 ΔPΔΑΔΚ Editing team in a proposal submitted by the Dept. of Chemistry under the title ―Infrastructure Equipment for the upgrade and reformation of the undergraduate curriculum and the educational process in the Department of Chemistry». 2004 ΠΔΝΔD. Scientific Supervisor in a proposal submitted in the field of «Development of bioanalytical methods for determination of heavy metals ions and species by atomic spectrometry, in human blood and its fractions».

18. PARICIPATION IN CONFERENCES

I have participated in the following International and national conferences and Symposia. In these I have presented the works with code numbers A1 to A111). Documents of these can be found in the CD, Part IX, Cp. 18. 1 4th International Symposium on “Environmental Pollution and its Impact on Life in

the Mediterranean Region” (MESAEP). Kavala, 6-11 September 1987. Presentation Α1.

2 12ν Panhellenic Conference in Chemistry, Chemical Education. Thessaloniki, 21-25 November 1988. Presentations Α2, Α4.

3 1ν Conference of Environmental Science and Technology. Mytilini, Greece, September 1989. Presentations Α5, Α6, Α7.

4 13ν Panhellenic Conference in Chemistry. Athens, 21-25 October 1991. Presentation Α8.

5 Annual Conference of Organization for the Restoration and Protection of the Environment in the '90s. Aristotle University, Thessaloniki, 19-21 August 1992. Presentation Α10.

6 Symposium «Quality Assurance in Analytical Laboratories». Society of North Greece Industries – Lab. of Analytical Chemistry, AUTH. Thessaloniki, 15 July 1993. Presentation Α11.

7 Workshop on "Trace Element Determination in Environmental and Food Matrices" European Commission, BCR Measurements and Testing Directorate Thessaloniki, 9-11 October 1994. Presentation Α12.

8 15ν Panhellenic Conference in Chemistry “Chemistry and Development”. Thessaloniki 6-10 December 1994. Presentations Α15, Α16, Α17, Α18, Α19.

9 6ν Panhellenic Conference of Greek Hydrotechnic Society. Thessaloniki, 22-26 May 1995. Presentation Α20.

10 Panhellenic Conference “Food Quality and Consumers Demands after 2000”. Thessaloniki, 5-6 May 1995. Presentation Α22.

11 5ν Oceanography and Fishery Conference. Kavala 15-18 April 1997. Presentation Α24, Α25.

12 Symposium of ELGA on “Introduction of new technologies in ELGA. The challenge of 2000”. Thessaloniki 31 March - 1 April 1997. Presentations Α26, Α27, Α28.

13 Environment and Education Conference Prefecture of Thessaloniki and ΑΠΘ-ΣΔΗΘ-MESAEP Thessaloniki, 16-18 October 1997. Presentation Α30.

14 2ν Panhellenic Conference in Enology. ΔΚΔΦΔ ΓΖΜΟΚΡΗΣΟ, 8-10 Μαϊοσ 1998. Presentation Α31.

15 1st Internat. Conference of Chemical Societies of South East European Countries Halkidiki, 1-4 June 1998. Presentation Α32, Α33.

16 1st Balkan Conference on “Education and Research within the frame of the

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substainable development. The case of the environment. Thessaloniki, 19-21 November 1998. Presentation Α35.

17 1st International Conference on “Instrumental Methods of Analysis. Modern Trends and Applications.

Chalkidiki, 19-22 September 1999. Presentations Α37, Α38. 18 3rd Euroconference on “Environmental Analytical Chemistry.

Environmental Αnalytical Chemistry for the 21st Century”. Chalkidiki, 9-15 October 1999. Presentations Α39, Α40. 19 18o Panhellenic Conference in Chemistry «Instrumental analysis-Accrediation».

Piraeus 10-13 March 2001. Presentations Α42, Α43, Α44. 20 2nd International Conference on Instrumental Methods of Analysis.

IMA 2001, Ioannina, 5-8 September 2001. Presentations Α45, Α46, Α47. 21 1ν Environmental Conference of Macedonia.

Thessaloniki, 1-4 Μαρηίοσ 2002. Presentations Α48, Α49. 22 Symposium for Strymonikos Gulf, Lakes Koronia and Volvi. Saint George Municipality of north Greece, 14 April 2002. 23 3rd Aegean Analytical Chemistry Days (3rd AACD). Lesbos, Polichnitos, Greece, 29 September - 3 October 2002. Presentations Α51, Α52. 24 3nd International Conference on Instrumental Methods of Analysis.

IMA 2003, Thessaloniki, 23-27 September 2003. Presentations Α55,Α56,Α57,Α58,Α59,Α60 25 8ν Panhellenic Conference in Chemistry of Greece and Cyprus.

Thessaloniki, 10-13 December 2004. Presentations Α64, Α65, Α66. 26 4th International Conference on Instrumental Methods of Analysis.

IMA 2005, Iraklion Crete, 2-6 October 2005. Presentations Α67, Α68, Α69, Α70, Α71, 72. 27 5th Aegean Analytical Chemistry Days (5th AACD). Thessaloniki, Greece, 5-8 October 2006. Presentations Α73, Α74, Α75, Α76, Α77. 28 11th Workshop on Progress of Analytical Methodologies for Trace Metal Speciation

(TraceSpec 2007)”. Munster, Germany, 4-7 September 2007. Presentation Α78.

29 5th International Conference on Instrumental Methods of Analysis (ΙΜΑ 2007). IMA 2007, Rio Patras, 30 Sept – 3 Oct 2007. Presentations Α79, Α80, Α81.

30 14th Biennial National Atomic Spectrometry Symposium. 14th BNASS 2008, Brighton, UK, 7 – 9 July 2008. Presentations Α83, Α84.

31 5th International Conference on Energy, Environment, Ecology and Sustainable Development EEESD 2009, WSEAS International Conference. Vouliagmeni, Athens, 28 – 30 Sept 2009. Presentation Α87.

32 6th International Conference on Instrumental Methods of Analysis (ΙΜΑ 2009). IMA 2009, Athens, 4 – 8 Oct 2009. Presentations Α88, Α89, Α90, Α91, Α92, Α93.

33 14th Hellenic Symposium (HELMED 2010). Thessaloniki, 23 – 25 April 2010. Presentations Α94, Α95, Α96.

34 7th Aegean Analytical Chemistry Days (7th AACD). Lesvos, Greece, 29 Sept -3 October 2010. Presentations Α97, Α98, Α99, Α100, Α101. 35 36th International Conference on Environmental Analytical Chemistry

36th IAEAC, Rome, 5 – 9 October 2010. Presentation Α102. 36 2nd International Conference on Biochemistry and Medical Chemistry

2nd BIOMEDCH11, University of Cambridge, 23 – 25 February 2011. Presentation Α103. 37 7th International Conference on Instrumental Methods of Analysis (ΙΜΑ 2011).

IMA 2011, Chania, Crete, 18 – 23 Sep 2011. Presentations Α104, Α105, Α106. 38 Diagnostic Techniques in the Science of Conservation of Art Objects.

Center of Research Results, AUTH, Thessaloniki, 17 – 19 February 2012. 39 12th Flow Analysis International Conference. Thessaloniki, Greece, 23-28 September 2012. Presentations Α108, Α109, Α110, Α111.

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19. PARTICIPATION IN SCIENTIFIC SEMINARS

I have attended the following scientific and technological seminars regarding the development and

use of modern instrumentation used in Analytical Chemistry. Documents of these can be found in the CD, Part IX, Cp. 19.

Μ1 Fundamentals, applications and Instrumentation of Inorganic Analysis (ICP-AES,

ICP-MS). Athens, 29 May 2002, Perkin Elmer Instruments. Μ2 Φαζκαηνκεηξία Μαδώλ κε Δπαγσγηθά πδεπγκέλν Πιάζκα (ICP-MS)

Thessaloniki, 10 November 2004, Hellamco. Μ3 Eθαξκνγή ηεο Οδεγίαο 2000/60/ΔΔ γηα λεξά ζηελ Διιάδα θαη Δπξσπατθή εκπεηξία.

1ο Environemntal Symposium, Thessaloniki, 11-12 November 2004. M4 4th Austrian Proteome Research Symposium, 20-21 September 2006. Μ5 Gas Chromatography and Mass Spectrometry (GC-MS). Hellamco.

Thessaloniki, 2-3 November 2006, Μ6 ARTAX Non-Destructive Analysis of Cultural Heritage Artefacts. Μicro XRF. 7 March

2007, Αλαισηηθές σζθεσές, Archaeological Museum of Thessaloniki. Μ7 PANanalytical and Hellamco, XRF and XRD. 12 Novemebr 2007, Thessaloniki

Μacedonia Palace. Μ8 ICP/ ICP MS, Perkin Elmer, Αληηει. 22 November 2007, Panorama. Μ9 Workshop on Holistic Analytical Technologies for Systems Biology Studies, 30-31

October 2008, Telloglio, Thessaloniki. Μ10 EcoAnalytix. ICPMS and Atomic Spectroscopy, Perkin Elmer, Αληηει. Hyatt, 6

November 2008, Thessaloniki. Μ11 Liquid Chromatography and Mass Spectrometry, DIONEX and BRUKER, Αλαισηηθές

σζθεσές, 12 November 2008, Capsis Hotel, Thessaloniki. Μ12 The role of Chromatography in food quality and safety (Perkin Elmer) - Total

solutions in modern mass spectrometry (Αgilent Technologies) - Spectrometric methods for the determination of metals by ICP-MS (ALS S.A.), 7 October 2009, Σitania Hotel, Athens.

20. INTERNATIONAL BOOK

Ν 1 “Inductively Coupled Plasma Atomic Emission Spectrometry. A model multielement

technique for modern laboratories” George Zachariadis NOVA Science Publishers, New York, USA, 2012 pp 260. A file of this book can be found in the CD, Part IX, Cp. 20.

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21. TUTORIAL BOOKS

From the tutorial books listed below, Β3, Β5, Β6, Β7, Β8, Β9, Β10, Β11, Β12 were teached or still are teached in the Department of Chemistry of Aristotle University. Β4, Β7, Β8 are teached in the Department of Pharmaceutics. Β1 and Β4 in the departments of Biology, Geology and Agriculture. Β2 in ΗΔΚ Institutes. Β4 in Technological and Educational Institute. Β7 in Academy and in Department of Chemistry in Ioannina and Chemical Engineering Patras. See in the CD, Part VIII. Β1 Analytical Chemistry. Introduction- Qualitative and Quantitative Analysis

(Αλαιπηηθή Υεκεία. Eηζαγσγή - Πνηνηηθή θαη Πνζνηηθή Αλάιπζε) D.G. Themelis, G.Α. Zachariadis, Simoni – Chatzipantou Publishing, Thessaloniki, 1994, pp.226.

Β2 Basics of General Chemistry. (ηνηρεία Γεληθήο Υεκείαο) G.Α. Zachariadis, ΙΕΚ Publishing, Thessaloniki, 1994, pp. 195.

Β3 Laboratory excercises of Instrumental Chemical Analysis (Δξγαζηεξηαθέο Αζθήζεηο Δλόξγαλεο Υεκηθήο Αλάιπζεο) Editor J. Papadoyannis. UV-Vis Spectrophotometry: G.Α. Zachariadis, Simoni – Chatzipantou Publishing, Thessaloniki, 1994. Edition, 2000.

Β4 Analytical Chemistry (Αλαιπηηθή Υεκεία) D.G. Themelis, G.Α. Zachariadis, ΕHTH Publishing. Thessaloniki,1998, 4ε Edition 2003, pp. 412.

Β5 Students notes in Advanced Quantitative Analysis (εκεηώζεηο Πξνρσξεκέλεο Πνζνηηθήο Αλάιπζεο) I.Α. Stratis, Γ.Α. Εαταρηάδες. AUTH Publishing, Thessaloniki, 1998, pp. 110.

Β6 Student Notes in Spectroscopic methods of Analysis (εκεηώζεηο Φαζκαηνζθνπηθώλ Μεζόδσλ Αλάιπζεο) I.Α. Stratis, D.G. Themelis, G.Α. Zachariadis, G. Theodoridis. AUTH Publishing, Thessaloniki,, 2001, pp. 358.

Β7 Introduction to Quantitative Analytical Chemistry. (Eηζαγσγή ζηελ Πνζνηηθή Υεκηθή Αλάιπζε)

(Αρτές, Χημειομεηρία, Μεθοδολογία, Καηεργαζίες) Α.Ν. Voulgaropoulos, G.Α. Zachariadis, I.Α. Stratis, ΕHTH Publishing. Thessaloniki, 1ε Edition 1999, Next Edition 2004, pp. 363.

Β8 Laboratory Methods of Quantitative Analytical Chemistry (Δξγαζηεξηαθέο Μέζνδνη Πνζνηηθήο Υεκηθήο Αλάιπζεο)

(Σηαθμική ανάλσζη και Τιηλομεηρία) I.Α. Stratis, G.Α. Zachariadis, Α.Ν. Voulgaropoulos ΕHTH Publishing. Thessaloniki, 1ε Edition 2000, Next Edition 2004, pp. 306.

Β9 Instrumental Chemical Analysis II (Δλόξγαλε Υεκηθή Αλάιπζε ΙΙ) I.Α. Stratis, D.G. Themelis,, G.Α. Zachariadis, Α. Αnthemidis,, Α. Economou. ΑΠΘ, ΕHTH Publishing, Thessaloniki, 2004, pp. 489.

Β10 Special Methods for Separation and Chemical Analysis (Δηδηθέο κέζνδνη δηαρσξηζκνύ θαη ρεκηθήο αλάιπζεο) I.Α. Stratis, G.Α. Zachariadis, V. Samanidou, G. Theodoridis. ΑΠΘ, ΕHTH Publishing, Thessaloniki, 2004, pp. 398.

Β11 Quantitative Chemical Analysis. Principles and laboratory excercises (Πνζνηηθή Υεκηθή Αλάιπζε. Αξρέο θαη εξγαζηεξηαθέο αζθήζεηο)

Α. Αnthemidis, Α.Ν. Voulgaropoulos, G.Α. Zachariadis, I.Α. Stratis ΕHTH Publishing, Thessaloniki, 2012, pp. 588.

Β12 Metrology, Chemometrics and Quality Control. In the Editor. (Μεηξνινγία ρεκεηνκεηξία θαη έιεγρνο πνηόηεηαο, Τπό έθδνζε)

G.Α. Zachariadis, Α.Ν. Voulgaropoulos, S. Girousi

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22. MY DOCTORAL THESIS

Γ1 Determination of mercury compounds by Cold vapour Atomic Absorption Spectrometry – Optimization by Factorial Design. G. Zachariadis. Doctoral Thesis, Department of Chemistry, Aristotle University. Thessaloniki, 1991, pp. 176. Scanned version of this can be found in the CD, Part VI.

23. PUBLISHED PAPERS IN PEER REVIEWED JOURNALS

PDF files of these can be found in the CD, Part III, with the code names Δ1, Δ2, ….. Γ1 Critical comparison of decomposition procedures for atomic absorption

spectrometrical analysis of prehistoric ceramics. J. Stratis, G. Zachariadis, E. Dimitrakoudi and V. Simeonov. Fresenius Journal of Analytical Chemistry, 1988, 331, 725-729.

Γ2 Preliminary environmental study of New Karvali bay (Kavala Gulf, North Greece). K. Ouzounis, G. Zachariadis and J. Stratis. Toxicological and Environmental Chemistry, 1989, 20-21, 227-232.

Γ3 Environmental study on chemical pollution of the lake Koronia in Northern Greece. G. Vassilikiotis, J. Stratis, A. Voulgaropoulos, G. Zachariadis and V. Michalopoulou. Journal of Environmental Science and Health, 1990, Α25, 611-620.

Γ4 Determination of chemical pollution parameters of the river Axios in Norther Greece. G. Vassilikiotis, A. Voulgaropoulos, J. Stratis, V. Michalopoulou and G. Zachariadis. Journal of Environmental Science and Health, 1991, Α26, 621-633.

Γ5 Distribution of heavy metals in sediments and aquatic plants of river Pinios (Central Greece). T. Sawidis, J. Stratis and G. Zachariadis. Σhe Science of the Total Environment, 1991, 102, 261-266.

Γ6 Optimization of Cold Vapour Atomic Absorption Spectrometric Determination of Mercury (CVAAS) with and without amalgamation by subsequent use of complete and fractional factorial designs with one-at-a-time experiment and modified Simplex methods. G. Zachariadis and J. Stratis. Journal of Analytical Atomic Spectrometry, 1991, 6, 239-245.

Γ7 Mosses as biological indicators for monitoring of heavy metal pollution. T. Savidis, G. Zachariadis, J. Stratis and E. Ladoukakis. Fresenius Environmental Bulletin, 1993, 2, 193-199.

Γ8 Furazolidone determination at random specimens of consumable bovine and pig muscles by HPLC. Caniou, H. Tsoukali, P. Epivatianos and G. Zachariadis. Journal of Environmental Science and Health. 1993, Α28, 645-650.

Γ9 Separation and determination of five penicillins by reversed phase HPLC. Caniou, G. Zachariadis and J. Stratis. Journal of Liquid Chromatography, 1993, 16, 2891-2897.

Γ10 Separation and determination of carbadox, nitrofurazone, nitrofurantoin, furazolidone and furaltadone in their mixtures by Thin Layer and High Performance Liquid Chromatography. Caniou, G. Zachariadis, G. Κaligas, H. Tsoukali and J. Stratis. Journal of Liquid Chromatography, 1994, 17, 1385-1398.

Γ11 Thin Layer and High Performance Liquid Chromatographic determination of histamine in fish tissues. G. Kalligas, I. Kaniou, G. Zachariadis, H. Tsoukali, P. Epivatianos Journal of Liquid Chromatography, 1994, 17, 2457-2468.

Γ12 Heavy metal pollution in the river Evros, (Southern Δurope). K. Ouzounis, G. Papageorgiou, J. Stratis and G. Zachariadis. Environmental Education and Information, 1994, 13, 31-44.

Γ13 Evaluation of chemical parameters in Aliakmon river, Northern Greece. Part I: Quality characteristics and nutrients.

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T. Kouimtzis, C. Samara, D. Voutsa and G. Zachariadis. Journal of Environmental Science and Health, 1994, Α29, 2115-2126.

Γ14 Evaluation of chemical parameters in Aliakmon river, Northern Greece. Part II: Dissolved and suspended heavy metals. D. Voutsa and G. Zachariadis, K. Samara, T. Kouimtzis. Journal of Environmental Science and Health, 1995, 30, 1-13.

Γ15 Determination of mercury by ETAAS using different chemical modifiers. Η. Karadjova, P. Mandjukov, S. Tsakovski, V. Simeonov, J. Stratis, G. Zachariadis. Journal of Analytical Atomic Spectroscopy, 1995, 10, 1065-1068.

Γ16 A study of air pollution with heavy metals in Thessaloniki using trees as biological indicators. T. Sawidis, A. Marnasidis, G. Zachariadis and I. Stratis. Archives of Environmental Contamination and Toxicology, 1995, 28, 118-124.

Γ17 Atomic Absorption Spectrometrical determination of molybdenum in aqueous tetrathiomolybdate solutions. Α. Voulgaropoulos, A. Agiannidis, G. Zachariadis, J. Stratis and S. Giroussi Fresenius Journal of Analytical Chemistry, 1995, 351, 139-140.

Γ18 Classification of Macedonian Early Bronze Age ceramics by the use of their various chemical and technological features. J.A. Stratis, G.A. Zachariadis, V.D. Simeonov Fresenius Journal of Analytical Chemistry, 1995, 351, 697-699.

Γ19 Determination of chloramphenicol residues in meat tissues High Performance Liquid Chromatography. Kaniou, G. Kalligas, Δ. Νikolaides, G. Zachariadis, J. Stratis Journal of Liquid Chromatography, 1995, 18, 3519-3527.

Γ20 Selective spectrophotometric determination of Co(II) using 2,2’-dipyridyl-2-pyridyl Hydrazone (DPPH) and a Flow Injection manifold. D.G. Themelis, G.A. Zachariadis, J.A. Stratis. Analyst, 1995, 120, 1593-1598.

Γ21 Heavy metal bioaccumulation in lichens from Macedonia in Northern Greece. T. Sawidis, M.K. Chettri, G.A. Zachariadis, J.A. Stratis, M.D. Seaward. Toxicological and Environmental Chemistry, 1995, 50, 157-166.

Γ22 Heavy metals in aquatic plants and sediments from water systems in Macedonia, Greece. T. Savidis, M.K. Chettri, G.A. Zachariadis, J.A. Stratis. Ecotoxicology and Environmental Safety, 1995, 32, 73-80.

Γ23 Critical comparison of wet and dry digestion procedures for trace metal analysis of meat and fish tissues. G.A. Zachariadis, J.A. Stratis, I. Kaniou and G. Kalligas. Mikrochimica Acta, 1995, 119, 191-198.

Γ24 Chemometrical classification of biomonitoring analytical data for heavy metals. Part I. Trees (leaves) as bioindicators. J. Stratis, G. Zachariadis, V. Simeonov, P. Mandjukov, T. Sawidis. Toxicological and Environmental Chemistry, 1996, 54, 29-37.

Γ25 Chemometrical approaches to treat analytical data from aquatic macrophytes and marine algae.

J.A. Stratis, V. Simeonov, G.A. Zachariadis, T. Savidis, P. Mandjukov, S. Tsakovski. Fresenius Journal of Analytical Chemistry, 1996, 355, 65-70.

Γ26 Evaluation of the toxic content of sludges produced during the biological treatment of municipal and industrial wastewaters.

D. Voutsa, G. Zachariadis, C. Samara, Th. Kouimtzis. Journal of Environmental Science and Health, 1996, 31, 657-671. Γ27 Evaluation of the quality of municipal and industrial wastewater sludges for

agricultural purposes. D. Voutsa, G. Zachariadis, N. Gantidis, C. Samara, Th. Kouimtzis. Fresenius Environmental Bulletin, 1996, 5, 1-6. Γ28 Uptake of heavy metal by living and dead Cladonia Thalli.

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M.K. Chettri, T. Sawidis, G.A. Zachariadis, J.A. Stratis Εnvironmental and Experimental Botany, 1997, 37, 39-52.

Γ29 Soxhlet extraction and acid digestion methods for silver determination in soils by flame and electrothermal Atomic Absorption Spectrometry. I.N. Papadoyannis, M.K. Sofoniou, V. F. Samanidou, G.A. Zachariadis, A.C Zotou, H.A. Constantinidou and S.E. Tsiouris. Instrumentation Science and Technology, 1997, 25, 283-296.

Γ30 Flame AAS and UV-VIS determination of cobalt, nickel and palladium using the synergetic effect of 2-benzoylpyridine-2-pyridylhydrazone and thiocyanate ions. G.A. Zachariadis, D.G. Themelis, D.J. Kosseoglou and J.A. Stratis. Talanta, 1998, 47, 161-167.

Γ31 Electrothermal atomic absorption spectrometric determination of aluminium, cadmium, chromium, copper, iron, manganese, nickel, and lead in olive oils. Η. Karadjova, G. Zachariadis, G. Boskou, J. Stratis Journal of Analytical Atomic Spectroscopy, 1998, 13, 201-204.

Γ32 Chemometrical classification of biomonitoring analytical data for heavy metals. Part II. Mosses as bioindicators. S. Tsakovski, V. Simeonov, Th. Sawidis, G. Zachariadis, J. Stratis Toxicological and Environmental Chemistry, 1999, 69, 287-294.

Γ33 Chemometrical classification of biomonitoring analytical data for heavy metals. Part III. Lichens as bioindicators. J. A. Stratis, S. Tsakovski, V. Simeonov, G. Zachariadis, Th. Sawidis, Toxicological and Environmental Chemistry, 1999, 69, 295-304.

Γ34 Effects of cadmium exposure on bioaccumulation and larval growth in the frog Rana ridibunda. N.S. Loumbourdis, P. Kyriakopoulou-Sklavounou, G. Zachariadis. Environmental Pollution, 1999, 104, 429-433.

Γ35 Use of Macroalgae as Biological Indicators of heavy metal pollution in Thermaikos Gulf, Greece. K. Fytianos, E. Evgenidou, G. Zachariadis. Bulletin of Environmental Contamination Toxicology, 1999, 62, 630-637.

Γ36 Spectrophotometric determination of phenols and cyanides after distillation from natural waters.

M.K.Sofoniou, G.A.Zachariadis, A.N.Anthemidis, Th.A. Kouimtzis. International Journal of Environmental Analytical Chemistry, 2000, 78, 353-365.

Γ37 Profile of heavy metals distribution in water and sediment samples from river Evros (Southeast Europe).

G. Vasilikiotis, G. Zachariadis, J. Stratis, F. Vosniakos Journal of Environmental Protection and Ecology, 2000, 1, 58-62. Γ38 SEM-EDXRF Studies of the behavior of two chemical modifiers based on Platinum

and ηungsten, and their effect in ETAAS determination of lead. G.A. Zachariadis, S.A. Sklavounos, P.B. Mandjukov, J.A. Stratis. Microchimika Acta, 2001, 136, 115-122.

Γ39 Determination of lead, cadmium and mercury in surface sediments and mussels. G.A.Zachariadis, A.N.Anthemidis, I.Caniou, J.A.Stratis. International Journal of Environmental Analytical Chemistry, 2001, 80, 153-166.

Γ40 A study of metal distribution from lignite fuels using trees as biological monitors. T. Sawidis, M.K. Chettri, A. Papaioannou, G. Zachariadis, J. Stratis.

Ecotoxicology and Environmental Safety, 2001, 48, 27-35. Γ41 On-line solid phase extraction system using PTFE packed column for the flame

atomic absorption spectrometric determination of copper in water samples. A.N. Anthemidis, G.A. Zachariadis, J.A. Stratis.

Talanta 2001, 54, 935-942. Γ42 Trace metal concentrations in marine macroalgae from different biotopes in the

Aegean Sea. T. Sawidis, M.T. Brown, G. Zachariadis, J. Stratis Εnvironment International, 2001, 27, 43-47.

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Γ43 Accumulation of heavy metals in vegetables grown in an industrial area in relation to soil. K. Fytianos, G. Katsianis, P. Triantafyllou, G. Zachariadis. Bulletin of Environmental Contamination Toxicology, 2001, 67, 423-430.

Γ44 Determination of mineral content of active dry yeast used in pharmaceutical formulations.

G.A. Zachariadis, E.S. Raidou, D. Themelis, J.A. Stratis. Journal of Biomedical and Pharmaceutical Analysis, 2002, 28, 463-473.

Γ45 Flame atomic absorption spectrometric determination of chromium (VI) by on-line preconcentration system using a PTFE packed column. A.N. Anthemidis, G.A. Zachariadis, J. Cougoulis, J.A. Stratis Σalanta, 2002, 57, 15-22.

Γ46 Assesment of the quality characteristics of Pinios River, Greece. K. Fytianos, A. Siumka, G. Zachariadis, S. Beltsios Water, Air and Soil Pollution, 2002, 136, 317-329.

Γ47 Determination of lead by on-line solid phase extraction using a PTFE micro-column and flame atomic absorption spectrometry. G.A. Zachariadis, A.N. Anthemidis, P. Bettas, J.A. Stratis Σalanta, 2002, 57, 919-927.

Γ48 Soil silver content of agricultural areas subjected to cloud seeding with silver iodide. S.E. Tsiouris, F.A. Aravanopoulos, I.N. Papadoyannis, M.K.Sofoniou, N. Polyzopoulos, M. Christodoulou, V.F. Samanidou, G.A. Zachariadis, H.A. Constantinidou

Fresenius Environmental Bulletin, 2002, 11, 697-702. Γ49 On-line preconcentration and determination of copper, lead and chromium(VI) using

unloaded polyurethane foam packed column by flame atomic absorption spectrometry in natural waters and biological samples. A.N. Anthemidis, G.A. Zachariadis, J.A. Stratis Σalanta, 2002, 58, 831-840.

Γ50 Cobalt ultra trace on-line preconcentration and determination using a PTFE turnings packed column and electrothermal atomic absorption spectrometry. Applications in natural waters and biological samples N. Anthemidis, G.A. Zachariadis, J.A. Stratis Journal of Analytical Atomic Spectrometry, 2002, 17, 1330-1334.

Γ51 Evaluation of different calibration methods in inductively coupled plasma atomic emission spectrometric analysis of certified glass materials and archaeological glass specimens G.A. Zachariadis, A.N. Anthemidis, J.A. Stratis

Journal of Analytical Atomic Spectrometry, 2003, 18, 358-366. Γ52 Gallium trace on-line preconcentration / separation and determination using a

polyurethane foam mini-column and flame atomic absorption spectrometry. Application in 22rocedure alloys, natural waters and urine. N. Anthemidis, G.A. Zachariadis, J.A. Stratis Talanta 2003, 60, 929-936.

Γ53 On-line preconcentration of mercury using an integrated column / gas-liquid separator (PCGLS) and cold vapor atomic absorption spectrometry G.A. Zachariadis*, A.N. Anthemidis, M. Karpouzi, J.A. Stratis Journal of Analytical Atomic Spectrometry, 2003, 18, 1274-1278.

Γ54 Development of an on-line solvent extraction system for electrothermal atomic absorption spectrometry utilizing a new gravitational phase separator. Determination of cadmium in natural waters and urine samples N. Anthemidis, G.A. Zachariadis, J.A. Stratis Journal of Analytical Atomic Spectrometry, 2003, 18, 1400-1403.

Γ55 Assesment of the surface water quality in Northern Greece V. Simeonov, J.A. Stratis, C. Samara, G. Zachariadis, D. Voutsa, A. Anthemidis, M. Sofoniou, Th. Kouimtzis Water Research, 2003, 37, 4119-4124.

Γ56 Soil silver mobility in areas subjected to cloud seeding with AgI

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S.E. Tsiouris, F.A. Aravanopoulos, I.N. Papadoyannis, M.K. Sofoniou, V.F. Samanidou, G.A. Zachariadis, E. Constantinidou. Fresenius Εnvironental Bulletin, 2003, 12, 1059-1063.

Γ57 On-line liquid-liquid extraction system using a new phase separator for flame atomic absorption spectrometric determination of ultra-trace cadmium in natural waters N. Anthemidis, G. A. Zachariadis, Ch. G. Farastelis, J. A. Stratis

Talanta, 2004, 62, 437-443. Γ58 Microwave-assisted versus conventional decomposition procedures applied on a

ceramic potsherd standard reference material by inductively coupled plasma atomic emission spectroscopy D.N. Papadopoulou, G.A. Zachariadis, A.N. Anthemidis, N.C. Tsirliganis, J.A. Stratis

Analytica Chimica Acta 2004, 505, 173-181. Γ59 Human recombinant mutated forms of the mitochondrial COΥ assembly Sco2

protein differ from wild-type in physical state and copper binding capacity. P.F. Foltopoulou, G.A. Zachariadis, A.S. Politou, A.S. Tsiftsoglou, L.C. Papadopoulou Molecular Genetics and Metabolism, 2004, 81, 225-236.

Γ60 Time-based injection on-line preconcentration cold vapour generation procedure for ultra-trace mercury determination with inductively coupled plasma atomic emission spectrometry. A.N. Anthemidis, G.A. Zachariadis, C.E. Michos, J.A. Stratis Analytical and Bioanalytical Chemistry, 2004, 379, 764-769.

Γ61 Development of a sequential injection system for trace mercury determination by cold vapour atomic absorption spectrometry utilizing an integrated gas liquid separator / reactor. N. Anthemidis, G. A. Zachariadis, J.A. Stratis Talanta, 2004, 64, 1053-1057.

Γ62 Direct determination of toxic metals in honey and sugars using inductively coupled plasma atomic emission spectrometry. M.D. Ioannidou, G.A. Zachariadis*, A.N. Anthemidis, J.A. Stratis Talanta, 2004, 65, 92-97.

Γ63 Comparison of portable micro-X-ray spectrometry with inductively coupled plasma atomic emission spectrometry for the ancient ceramics analysis. D.N. Papadopoulou, G.A. Zachariadis, A.N. Anthemidis, N.C. Tsirliganis, J.A. Stratis Spectrochimica Acta Part B, 2004, 59, 1877-1884.

Γ64 Determination of arsenic(III) and total inorganic arsenic in water samples using an on-line sequential insertion system and hydride generation atomic absorption spectrometry. A.N. Anthemidis, G. A. Zachariadis, J.A. Stratis Analytica Chimica Acta, 2005, 547, 237-242.

Γ65 On-line speciation of mercury and methylmercury by cold vapor atomic absorption spectrometry using selective solid phase extraction.

G. A. Zachariadis*, A. N. Anthemidis, Δ. Daftsis, J.A. Stratis Journal of Analytical Atomic Spectrometry, 2005, 20, 63-65.

Γ66 Development and validation of routine analysis methods for the determination of essential, non-essential and toxic minor and trace elements in cereals and cereal flour samples by inductively coupled plasma – atomic emission spectrometry.

Momen Awwad, G. A. Zachariadis*, A. N. Anthemidis, J.A. Stratis Journal of AOAC, 2005, 88, 1797-1810.

Γ67 Optimized microwave-assisted decomposition method for multi-element analysis of glass standard reference material and ancient glass specimens by inductively coupled plasma atomic absorption spectrometry. G.A. Zachariadis*, E. Dimitrakoudi, A.N. Anthemidis, J.A. Stratis Talanta 2006, 68, 1448-1456.

Γ68 Development and optimization of a portable-micro-XRF method for the in situ multi-element analysis of ancient ceramics D.N. Papadopoulou, G.A. Zachariadis, A.Ν. Anthemidis, N.C. Tsirliganis, J.A. Stratis

Talanta 2006, 68, 1692-1699.

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Γ69 Investigation of four digestion procedures for multi–element determination of toxic and nutrient elements in legumes by inductively coupled plasma optical emission spectrometry Momen Awwad, G. A. Zachariadis*, A. N. Anthemidis, J.A. Stratis Anal. Chim. Acta, 2006, 565, 81-88

Γ70 Development of a rapid multi-element method of analysis of antitussive syrups by inductively coupled plasma atomic emission spectrometry and direct sample introduction. G.A. Zachariadis*, D. Kapsimali, J. Pharm. Biomed. Anal. 2006, 41, 1212-1219

Γ71 Use of fractional factorial design for optimization of digestion procedures followed by multi-element determination of essential and non essestial elements in nuts using ICP-OES technique. Momen Awwad, G. A. Zachariadis*, A. N. Anthemidis, J.A. Stratis Talanta, 2007, 71, 443-451 .

Γ72 Analytical performance of ETAAS method for Cd, Co, Cr and Pb determination in blood fraction samples. E.Daftsis, G.A. Zachariadis* Talanta, 2007, 71, 722-730.

Γ73 Development of a slurry introduction method for multi-element analysis of antibiotics by inductively coupled plasma atomic emission spectrometry using various types of spray chamber and nebulizer configurations. G.A. Zachariadis*, C. E. Michos J. Pharm. Biomed. Anal. 2007, 43, 951-958.

Γ74 Analytical performance of a multi-element ICP-AES method for Cd, Co, Cr, Cu, Mn, Ni and Pb determination in blood fraction samples E.Daftsis, G.A. Zachariadis* Microchimica Acta, 2008, 160, 405-411.

Γ75 Optimization and comparison of two digestion methods for multi-element analysis of certified reference plant materials by ICP–AES. Application of Plackett-Burman and Central Composite Designs. Momen Awwad, G. A. Zachariadis*, A. N. Anthemidis, J.A. Stratis Microchimica Acta, 2008, 160, 397-403.

Γ76 Arabino-Galactan Proteins from Pistacia lentiscus var. chia: isolation, characterization and biological function F. Kottakis, F. Lamari, Ch. Matragkou, G. Zachariadis, N. Karamanos, and T. Choli-Papadopoulou. AminoAcids, 2008, 34, 413-420.

Γ77 Evaluation and comparison of two combinations of pneumatic nebulizers and spray chambers for direct slurry aspiration and multielement analysis of infant milk powders by axial-viewing inductively coupled plasma atomic emission spectrometry G.A. Zachariadis*, L.I. Valianou Applied Spectroscopy, 2008, 62, 716-720.

Γ78 Use of slyrry suspension sample introduction technique in multielement analysis of multivitamin preparations by inductively coupled plasma atomic emission spectrometry G. A. Zachariadis*, A. F. Olympiou, J. Pharm. Biomed. Anal. 2008, 47, 541-546.

Γ79 Effect of sample matrix on sensitivity of mercury and methylmercury quantitation in human urine, saliva and serum using GC-MS G. A. Zachariadis*, D. C. Kapsimali, J. Sep. Sci. 2008, 31, 3884 – 3893

Γ80 Determination of butyl- and phenyltin compounds in human urine by HS-SPME after derivatization with tetraethylborate and subsequent determination by capillary GC with microwave-induced plasma atomic emission and mass spectrometric detection G. A. Zachariadis*, E. Rosenberg,

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Talanta, 2009, 78, 570-576. Γ81 Speciation of organotin compounds in urine by GC-MIP-AED and GC-MS after

ethylation and liquid-liquid extraction

G. A. Zachariadis*, E. Rosenberg, J. Chromatogr B, 2009, 877, 1140-1144.

Γ82 Internal standardization with yttrium spectral lines using axial-viewing inductively coupled plasma atomic emission spectrometry for plant certified reference materials G. A. Zachariadis*, C. Sarafidou, Microchimica Acta, 2009, 166, 77-81.

Γ83 Multi-element method for determination of trace elements in sunscreen by ICP-AES G. A. Zachariadis*, E. Sahanidou, J. Pharm. Biomed. Anal. 2009, 50, 342-348.

Γ84 Headspace and direct immersion solid phase microextraction procedures for selenite determination in urine, saliva and milk by gas chromatography mass spectrometry. D.C.Kapsimali, G.A.Zachariadis* J Chromatography B, 2009, 877, 3210-3214.

Γ85 On-line preconcentration and determination of nickel and zinc in natural water samples by flow injection - flame atomic absorption spectrometry using PTFE-turnings for column packing A.N. Anthemidis, G.A. Zachariadis, J.A. Stratis. Intern. J. Environ. Analyt. Chem., 2010, 90, 127-136.

Γ86 Comparison of tetraethylborate and tetraphenylborate for selenite determination in human urine by gas chromatography mass spectrometry, after headspace solid phase microextraction

D.C.Kapsimali, G.A.Zachariadis* Talanta 2010, 80, 1311-1317.

Γ87 A new approach to indophenol blue method for determination of ammonium in geothermal waters with high mineral contents N. M. Tzollas, G. A. Zachariadis*, A. N. Anthemidis. J. A. Stratis Intern. J. Environ. Analyt. Chem., 2010, 90, 115-126.

Γ88 Speciation of inorganic tin and tetramethyltin by headspace solid phase microextraction and gas chromatography mass spectrometry N. M. Tzollas, G.A.Zachariadis*, J Separation Science, 2010, 33, 1610-1616,

Γ89 Poly(etheretherketone)-turnings, a novel sorbent material for lead determination by flow injection flame atomic absorption spectrometry and factorial design optimization. A.N. Anthemidis, I.S.I. Adam, G.A. Zachariadis Talanta, 2010,81, 996-1002.

Γ90 A review of recent developments in sample pretreatment, separation and hyphenated atomic and mass spectrometric techniques for organoselenium speciation in biological liquids G.A. Zachariadis*, D.C. Kapsimali, E. Rosenberg Current Organic Chemistry, 2010, 14, 2282-2299.

Γ91 An overview of the use of yttrium for internal standardization in Inductively Coupled Plasma – Atomic emission spectrometry. G. A. Zachariadis*, C. Vogiatzis Applied Spectroscopy Reviews, 2010, 45, 220-239.

Γ92 Microwave-assisted acid extraction methodology for trace elements determination in mastic gum of Pistacia Lentiscus by Inductively Coupled Plasma Atomic Emission Spectrometry. G. A. Zachariadis*, E. Spanou Phytochemical Analysis, 2011, 22, 31-35.

Γ93 Analytical performance of a fast multi-element method for titanium and trace elements determination in cosmetics and pharmaceuticals by ICP-AES G. A. Zachariadis*, E. Sahanidou,

26

Central European J Chemistry, 2011, 9, 213-217. Γ94 Effect of traffic pollution on seed quality characteristics of Pinus brutia.

P. Ganatsa, M. Tsakaldimi, G. A. Zachariadis Environmental and Experimental Botany, 2011, 74, 157-161.

Γ95 An evaluation of the use of Yttrium and beryllium as internal standards in inductively coupled plasma emission spectrometry for untreated aqueous solutions in presence of high concentrations of organic solvents and matrices. G. A. Zachariadis*, C. Vogiatzis Journal of Analytical Atomic Spectrometry, 2011, 26, 2030 - 2038

Γ96 Ion chromatographic method for the determination of cations of group IA and IIA in water samples, pharmaceuticals and energy drinks by non-suppressed conductometric detection. G. A. Zachariadis*, A. Lyratzi, J. A. Stratis Central European Journal Chemistry, 2011, 9, 941-947.

Γ97 Hypersep-SCX micro-cartridge for on-line flow injection-inductively coupled plasma atomic emission spectrometry determination of trace elements in biological and environmental samples A. N. Anthemidis, G. Giakisikli, G.A. Zachariadis Analytical Methods 2011, 3, 2108-2114.

Γ98 Feasibility and effectiveness of inhaled carboplatin in NSCLC patients. P. Zarogoulidis, E. Eleftheriadou, I. Sapardanis, V. Zarogoulidou, H. Lithoxopoulou, T. Kontakiotis, N. Karamanos, G. Zachariadis, M. Mabroudi, A. Zisimopoulos, K. Zarogoulidis Investigational New Drugs, 2012, 30, 1628-1640.

Γ99 Speciation of Cr(III) and Cr(VI) using solid phase extraction and determination of total As by inductively coupled plasma atomic emission spectrometry. G. A. Zachariadis*, E. Trikas Intern J Environ. Analytical Chemistry 2012, 92, 385-391.

Γ100 Investigation of the HPLC-IT-TOF-MS Technique with Atmospheric Pressure Chemical Ionization for Speciation of Selenoaminoacids, Dimethyldiselenide, and Diphenyldiselenide D. Kapsimali, E. Rosenberg, G. A. Zachariadis* Analytical Letters, 2012, 45, 642-650.

Γ101 Headspace solid phase microextraction for terpenes and volatile compounds determination in mastic gum extracts, mastic oil and human urine by GC–MS G. A. Zachariadis*, A. Laggioli Analytical Letters, 2012, 45, 993-1003.

Γ102 Far infrared study of local impurity modes of Boron-doped PbTe. P. M. Nikolic, K. M. Paraskevopoulos, G. Zachariadis, O. Valasiadis, T.T. Zorba, S.S. Vujatovic, N. Nikolic, O. S. Aleksic, T. Ivetic, O. Cvetkovic, V. Blagojevic, M.V. Nikolich, Journal of Material Science, 2012, 47, 2384-2389.

Γ103 Determination of organolead and organotin compounds in urine and aqueous samples by GC-MIP-AED and GC-MS after ethylation and liquid-liquid extraction G. A. Zachariadis*, E. Rosenberg, J Sep Science, 2012, 35, 1132-1137.

Γ104 Storage stability studies for tributyltin determination in human urine samples using headspace SPME and gas chromatography and mass spectrometry. N. M. Tzollas, M. Nikolaou, E. Rosenberg, G. A. Zachariadis* Biomedical Chromatography, 2012, DOI 10.1002/bmc.2791

Γ105 Speciation study of trialkyl- and triphenyl tin by liquid chromatography using Ion Trap TOF tandem MS and atmospheric pressure chemical ionization. N. M. Tzollas, E. Rosenberg, G. A. Zachariadis*, Current Analytical Chem.y 2012, In press.

Γ106 Editorial. Metal Speciation and metallomics. G. A. Zachariadis* Current Analytical Chemistry 2013, Vol. 9, In press.

Γ107. Use of Dohelert-type designs in the optimization of a hybrid Electrospray Ionization Ion Trap TOF-MS for glutathione determination G. A. Zachariadis*, E. Rosenberg, Rapid Communication in Mass Spectrometry, In Press.

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24. CONFERENCE PRESENTATIONS

PDF files of these can be found in the CD, Part V, with the code names A1, A2, …..

Α1 Preliminary environmental study of New Karvali Bay.

Κ. Οuzounis, J. Stratis and G. Zachariadis. Abstracts of the 4th International Symposium on Environmental Pollution and its Impact on Life in the Mediterranean Region (MESAEP). Kavala, 6-11 September 1987.

Α2 Πξνζδηνξηζκόο παξακέηξσλ ρεκηθήο ξύπαλζεο ζηνλ Παγαζεηηθό θόιπν. Β. Μηταιοπούιοσ, Γ. Εαταρηάδες, Κ. Νηθοιάοσ, Γ. Γηακαληίδες θαη Α. Βοσιγαρόποσιος. Ππακηικά 12ος Πανελλήνιος ςνέδπιος Υημείαρ, Σόκος Β, ζει. 189-193. Θεζζαιολίθε, 21-25 Νοεκβρίοσ 1988.

Α3 Αλάπηπμε πξνγξάκκαηνο γηα κηα ηαρεία πξνζέγγηζε ζηα πξνβιήκαηα ζηηο εθρύιηζεο. Η. Α. ηράηες, Γ. Α. Εαταρηάδες θαη Α. Κ. Παπαδόποσιος. Ππακηικά ζςνεδπίος (Apple) Hellenic University Consortium, ζει. 153-155. Υαιθηδηθή 1988.

Α4 Θεσξεηηθή πξνζέγγηζε θαη βειηηζηνπνίεζε ζπλζεθώλ ππνινγηζκνύ ηνπ ιόγνπ θαηαλνκήο θαηά ηελ εθρύιηζε ζπκπιόθσλ ησλ κεηάιισλ κε ηελ ΦΑΑ.

Η. Α. ηράηες, Γ. Α. Εαταρηάδες θαη Α. Κ. Παπαδόποσιος. Ππακηικά 12ος Πανελλήνιος ςνέδπιος Υημείαρ, Σόκος Β, ζει. 177-182. Θεζζαιολίθε, 21-25 Νοεκβρίοσ 1988.

Α5 Μειέηε παξακέηξσλ ρεκηθήο ξύπαλζεο ηεο ιίκλεο ηνπ Αγίνπ Βαζηιείνπ. Γ. Βαζηιηθηώηες, Η. ηράηες, Α. Βοσιγαρόποσιος, Γ. Εαταρηάδες θαη Β. Μηταιοπούιοσ. Ππακηικά 1ος ςν. Πεπιβαλλονηικήρ Επιζηήμηρ και Σεσνολογίαρ, Σόκος Α, ζει. 325-334. Μσηηιήλε, επηέκβρηος 1989.

Α6 Πξνζδηνξηζκόο παξακέηξσλ ρεκηθήο ξύπαλζεο ζηνλ πνηακό Αμηό. Γ. Βαζηιηθηώηες, Α. Βοσιγαρόποσιος, Η. ηράηες, Β. Μηταιοπούιοσ θαη Γ. Εαταρηάδες. Ππακηικά 1ος ςν. Πεπιβαλλονηικήρ Επιζηήμηρ και Σεσνολογίαρ, Σόκος Α, ζει. 335-344. Μσηηιήλε, επηέκβρηος 1989.

Α7 Μεζπιίσζε θαη ηνμηθόηεηα ηνπ πδξαξγύξνπ θαη ησλ κνξθώλ ηνπ ζηνλ άλζξσπν θαη ζηηο ζαιάζζηνπο νξγαληζκνύο. Η. ηράηες, θαη Γ. Εαταρηάδες. Ππακηικά 1ος ςν. Πεπιβαλλονηικήρ Επιζηήμηρ και Σεσνολογίαρ, Σόκος Β, ζει.74-82. Μσηηιήλε, επηέκβρηος 1989.

Α8 Φαζκαηηθέο παξεκπνδίζεηο από νξγαληθνύο δηαιύηεο ζηνλ πξνζδηνξηζκό πδξαξγύξνπ κε ηε θαζκαηνκεηξία αηνκηθήο απνξξόθεζεο ςπρξνύ αηκνύ. Γ. Εαταρηάδες θαη Η. ηράηες. Ππακηικά 13ος Πανελλήνιος ςνέδπιος Υημείαρ, Σόκος Β, ζει. 399-403. Αζήλα, 21-25 Οθηωβρίοσ 1991.

Α9 Αλίρλεπζε θνπξαδνιηδόλεο ζε κπηθό ηζηό ρνίξσλ θαη βννεηδώλ κε ηε κέζνδν ζηηο πγξήο ρξσκαηνγξαθίαο πςειήο απόδνζεο. Η. Καληού, Δ. Σζούθαιε, Π. Δπηβαηηαλός, Γ. Εαταρηάδες Ππακηικά 4ος Πανελλήν. ςνέδπιος Επιζηήμηρ και Σεσνολογίαρ Σποθίμων, ζει. 259-264. Αζήλα, 25-27 Νοεκβρίοσ 1992.

Α10 Preliminary notes on a modified method for asbestos determination in surface waters. S. Α. Sklavounos, E. G. Pavlidou, B. K. Kyriakopoulos and G. A. Zachariadis Proceed. Of Annual Conference on Restoration and Protection of Environment ζει.38-41. Aristotle University, Thessaloniki, 19-21 August 1992.

Α11 Γηεξγαζηεξηαθέο δνθηκαζίεο ζηε ρεκηθή αλάιπζε. Γ. Εαταρηάδες. Ζκερίδα «Διαζθάλιζηρ Ποιόηηηαρ ζηα Αναλςηικά Επγαζηήπια» ύλδ.Βηοκεταληώλ Βορείοσ Διιάδος- Δργ. Αλαισηηθής Υεκείας, ΑΠΘ. Θεζζαιολίθε, 15 Ηοσιίοσ 1993

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Α12 Critical comparison of wet and dry digestion procedures for trace metal analysis of meat and fish tissues. G.A. Zachariadis, J.A. Stratis, I. Kaniou and G. Kalligas Workshop on “Trace Element Determinations in Environmental and Food Matrices”, European Commission, BCR Measurements and Testing Directorate Thessaloniki, 9-11 October 1994.

Α13 Determination of mercury by ETAAS using different chemical modifiers. Η.Karadjova, P. Mandjukov, S. Tsakovski, V. Simeonov, J. Stratis and G. Zachariadis. Abstracts of East European Furnace Symposium, EEFS. Warsaw, 4-7 September 1994.

Α14 Chemometrical approaches to treat analytical data from aquatic macrophytes and marine algae. J.A. Stratis, V. Simeonov, G.A. Zachariadis, T. Savidis, P. Madjukov, S. Tsakovski 2nd FAO/UNEP Sub-Regional Workshop on the Monitoring of Chemical Contaminants in Marine Biota for Trends. Lesbos Island, 19-22 October 1994.

Α15 Μειέηε δηαιπηνπνίεζεο πνιπηεξεθζαιηθνύ αηζπιελεζηέξα (ΡΔΣ) κε πγξή πέςε θαη πξνζδηνξηζκόο αληηκνλίνπ, ςεπδαξγύξνπ θαη καγγαλίνπ ζε κε ηε θαζκαηνκεηξία αηνκηθήο απνξξόθεζεο. Γ. Εαταρηάδες, Ρ. Παπή, . Πεηρίδες, Η. ηράηες Ππακηικά 15ος Πανελλήνιος ςνέδπιος Υημείαρ, Σόκος A, ζει. 139-143. Θεζζαιολίθε 6-10 Γεθεκβρίοσ 1994.

Α16 Πξνζδηνξηζκόο αληηκνλίνπ, ςεπδαξγύξνπ θαη καγγαλίνπ ζε πνιπηεξεθζαιηθό αηζπιελεζηέξα (ΡΔΣ) κε ηε θαζκαηνκεηξία αηνκηθήο απνξξόθεζεο. Γ. Εαταρηάδες, . Πεηρίδες, Ρ. Παπή, Η. ηράηες Ππακηικά 15ος Πανελλήνιος ςνέδπιος Υημείαρ, Σόκος A, ζει.144-148. Θεζζαιολίθε 6-10 Γεθεκβρίοσ 1994.

Α17 Πξνζδηνξηζκόο ζηδήξνπ θαη ραιθνύ ζε ειαηόιαδν κε πγξή πέςε θαη θαζκαηνκεηξία αηνκηθήο απνξξόθεζεο κε θνύξλν γξαθίηε. Β. Υ. ηώρος, Γ.Γ. Μπόζθοσ, Γ. Α. Εαταρηάδες, Η. Α. ηράηες Ππακηικά 15ος Πανελλήνιος ςνέδπιος Υημείαρ, Σόκος A, ζει.149-153. Θεζζαιολίθε 6-10 Γεθεκβρίοσ 1994.

Α18 Πξνζδηνξηζκόο ηρλνζηνηρείσλ ζε ειαηόιαδν ρσξίο πέςε κε ETAAS θαη νξγαληθνύο ρεκηθνύο ηξνπνπνηεηέο. Γ.Γ. Μπόζθοσ, Δ. Καραληδόβα, Γ. Α. Εαταρηάδες, Η. Α. ηράηες Ππακηικά 15ος Πανελλήνιος ςνέδπιος Υημείαρ, Σόκος A, ζει. 154-157. Θεζζαιολίθε 6-10 Γεθεκβρίοσ 1994.

Α19 Πξνζδηνξηζκόο ππνιεηκκάησλ ρισξακθεληθόιεο ζε θξέαο κε πγξή ρξσκαηνγξαθία πςειήο απόδνζεο. Η. Καληού, Δ. Νηθοιαϊδες, Δ. Σζούθαιε, Η. ηράηες, Γ. Εαταρηάδες Ππακηικά 15ος Πανελλήνιος ςνέδπιος Υημείαρ, Σόκος B, ζει.936-939 Θεζζαιολίθε 6-10 Γεθεκβρίοσ 1994.

Α20 Υαξαθηεξηζηηθά πνηόηεηαο ησλ λεξώλ ηνπ πνηακνύ Αιηάθκνλα. Γ. Βοσηζά, Γ. Εαταρηάδες, Κ. ακαρά, Θ. Κοσϊκηδής, . θιαβούλος Ππακηικά 6ος Πανελλήνιος ςνέδπιος Ελληνικήρ Τδποηεσνικήρ Ένωζηρ, ζει. 95-105. Θεζζαιολίθε, 22-26 Μαϊοσ 1995.

Α21 Αμηνιόγεζε ηνπ ηνμηθνύ πεξηερνκέλνπ ζηηο ιάζπεο πνπ παξάγεηαη θαηά ην βηνινγηθό θαζαξηζκό αζηηθώλ θαη βηνκεραληθώλ απνβιήησλ. Γ. Βοσηζά, Γ. Εαταρηάδες, Γ. Γθαληίδες, Κ. ακαρά, θαη Θ. Κοσϊκηδής Ππακηικά 4ος ςνεδπίος Πεπιβαλλονηικήρ Επιζηήμηρ και Σεσνολογίαρ, ζελ. 754-763. Λέζβος, επηέκβρηος 1995.

Α22 Αλαιπηηθνί πξνζδηνξηζκνί ηρλώλ θαδκίνπ θαη κνιύβδνπ ζε ηρζπξά, νζηξαθνεηδή θαη καιάθηα, κε ηε κέζνδν ζηηο θαζκαηνκεηξίαο αηνκηθήο απνξξόθεζεο. Γ. Καιιηγάς, Η. Καληού, Η. ηράηες, Γ. Εαταρηάδες Πανελλήνιο ςνέδπιο Ποιόηηηα ηων Σποθίμων, ΔΕΣΡΟΠ. Θεζζαιολίθε, 5-6 Μαίοσ 1995.

Α23 Δξεπλα θαη αλάπηπμε ζηηο κε ξππνγόλνπ, πςειήο απόδνζεο κπαηαξίαο ηόλησλ Li

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κε θάζνδν ιηζησκέλν ΜnO2. Γ. ηάπθας, Η. ακαράς, . Κόθθοσ, Υ. Μήηζας, Σ. Εορκπά, Κ. Παραζθεσόποσιος, Δ. Υαηδεθραληώηες, Η. ηοηκέλος, Α. Βοσιγαρόποσιος, Η. ηράηες, Α. Εοσκπούιες, Γ. Μοσκοσδηάς, . Γερούζε, Ν. Λαδαρίδες, Μ. οθολίοσ, Γ. Εαταρηάδες, Α. Αλζεκίδες. Ππακηικά 17ος Πανελλήνιος ςνέδπιος Υημείαρ, ζει. 627-630. Πάηρα 1-5 Γεθεκβρίοσ 1996.

Α24 Αλαιπηηθνί πξνζδηνξηζκνί βαξέσλ κεηάιισλ ζηα ηδήκαηα ζηηο ιίκλεο Κνξόληαο. Α. Αλζεκίδες, Γ. Εαταρηάδες, Η. ηράηες, Α. Βοσιγαρόποσιος, Γ. Βαζηιηθηώηες Ππακηικά 5ος ςμποζίος Ωκεανογπαθίαρ και Αλιείαρ, Σόκος ΗΗ, ζει. 313-316. Καβάια 15-18 Απρηιίοσ 1997.

Α25 Αλαιπηηθνί πξνζδηνξηζκνί βαξέσλ κεηάιισλ ζηα ηδήκαηα ηνπ πνηακνύ Αμηνύ. Γ. Εαταρηάδες, Α. Αλζεκίδες, Η. ηράηες, Α. Βοσιγαρόποσιος, Γ. Βαζηιηθηώηες Ππακηικά 5ος ςμποζίος Ωκεανογπαθίαρ και Αλιείαρ, Σόκος ΗΗ, ζει. 317-320. Καβάια 15-18 Απρηιίοσ 1997.

Α26 Πξνζδηνξηζκνο ηνπ ησδηνύρνπ αξγύξνπ ζε εδάθηθά, θπηηθά θαη δστθά δείγκαηα. Η. Παπαδογηάλλες, Μ. οθολίοσ, Β. ακαλίδοσ, Γ. Εαταρηάδες Διημεπίδα“Η ειζαγωγή νέων ηεσνολογιών ζηιρ Eλληνικέρ Γεωπγικέρ Αζθαλίζειρ. ελ. 18-20. Θεζζαιολίθε 31 Μαρηίοσ – 1 Απρηιίοσ 1997.

Α27 Πξνζδηνξηζκνο AgI ζην πεδίν. Απνηειέζκαηα. Η. Παπαδογηάλλες, Μ. οθολίοσ, Β. ακαλίδοσ, Γ. Εαταρηάδες, . Σζηούρες, Φ. Αραβαλόποσιος, Ν. Ποισδόποσιος, Δ. Κωλζηαληηλίδοσ. Διημεπίδα“Η ειζαγωγή νέων ηεσνολογιών ζηιρ Eλληνικέρ Γεωπγικέρ Αζθαλίζειρ. ελ. 24-26. Θεζζαιολίθε 31 Μαρηίοσ-1 Απρηιίοσ 1997.

Α28 Παζνινγναλαηνκηθέο επηπηώζεηο ηνπ AgI ζηα δώα. Η. Βιέκκας, Ν. Παπαϊωάλλοσ, Η. Παπαδογηάλλες, Μ. οθολίοσ, Θ. Ποσηατίδες, Β. ακαλίδοσ, Γ. Εαταρηάδες. Διημεπίδα: Η ειζαγωγή νέων ηεσνολογιών ζηιρ Eλληνικέρ Γεωπγικέρ Αζθαλίζειρ. ζελ.34-36. Θεζζαιολίθε 31 Μαρηίοσ-1 Απρηιίοσ 1997.

Α29 ENVIRO-SOFT: A network for environmental data base F. Vosniakos, P. Mentzelou, N. Zoumakis, G. Zachariadis, M. Katsantoni and G. Vasilikiotis. Abstracts of the 9th Intern. Symp. On Environmental Pollution and its Impact on Life in the Mediterranean Region (MESAEP). Italy, 4-9 October 1997.

Α30 Βαξέα θαη ηνμηθά κέηαιια ζηα λεξά Γ. Εαταρηάδες, Ππακηικά ζςνεδπίος «Πεπιβάλλον και Εκπαίδεςζη»

Νομαπσία Θεζζαλονίκηρ και ΑΠΘ-ΣΕΙΘ-MESAEP, ζελ. 126-131. Θεζζαιολίθε, 16-18 Οθηωβρίοσ 1997.

Α31 πγθξηηηθή κειέηε κεζόδσλ πξνζδηνξηζκνύ Na+, K+, Ca2+, Mg2+, NH4+, Cl- θαη SO4

2- ζε νίλνπο κε ηνληθή ρξσκαηνγξαθία, θαζκαηνκεηξία αηνκηθήο απνξξόθεζεο θαη πνηελζηνκεηξία.

Α. Θεοδωρίδες, Γ. Εαταρηάδες, . Γερούζε, Γ. Θεκειής, Η. ηράηες. 2ο Πανελλήνιο ςνέδπιο Οινολογίαρ, ΔΚΔΦΔ ΓΖΜΟΚΡΗΣΟ, 8-10 Μαϊοσ 1998.

Α32 Synthesis, characterization and electrochemical properties of Li2Mn2O4 for lithium batteries.

G. Zachariadis, A. Anthemidis, M. Sofoniou, J. Stratis, T. Zorba, S. Kokou, D. Siapkas. 1st Intern. Conference of the Chemical Societies of the South East European Countries, Chalkidiki, 1-4 June 1998.

Α33 Critical comparison of decomposition procedures for AAS determination of metals in yeast.

E.S. Raidou, G.A. Zachariadis, D. G. Themelis, J.A. Stratis. 1st Intern. Conference of the Chemical Societies of the South East European Countries. Chalkidiki, 1-4 June 1998.

Α34 πγθεληξώζεηο ηζηακίλεο ζε αιηεύκαηα θαη δεκόζηα πγεία. Η. Καληού, Φ.Σζάθαρες, Γ. Καιιηγάς, Γ. Εαταρηάδες. 1ο Πανελλήνιο ςνέδπιο Ιαηποδικαζηικήρ και Σοξικολογίαρ,

Θεζζαιολίθε 19-20 επηεκβρίοσ 1998.

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Α35 Profile of heavy metals distribution in water and sediment samples from river Evros G. Vasilikiotis, G. Zachariadis, J. Stratis, F. Vosniakos. 1st Balkan Conference on “Education and Research within the frame of the substainable development. The case of the environment”. Thessaloniki, 19-21 November 1998.

Α36 Use of macroalgae as biological indicators of heavy metal pollution in Thermaikos Gulf, Greece. K. Fytianos, E. Evgenidou, G. Zachariadis. 6ο ςνέδπιο Πεπιβαλλονηικήρ Επιζηήμηρ και Σεσνολογίαρ,

άκος 30 Ασγούζηοσ – 2 επηεκβρίοσ 1999. Α37 Investigations on chemical and structural changes of Pt- and W-based chemical

modifiers in ET-AAS by use of SEM-EDS techniques. G.A. Zachariadis, S.A. Sklavounos, P.B. Mandjukov, J.A. Stratis. Proceedings of 1st International Conference on “Instrumental Methods of Analysis. Modern Trends and Applications” Vol. I. pp. 124-128. Chalkidiki, 19-22 September 1999.

Α38 A quality study of surface waters of Macedonia, Greece: Heavy metals and other toxic elements. D. Voutsa, G. Zachariadis, A. Kouras, A. Anthemidis, C. Samara. Proceedings of 1st International Conference on “Instrumental Methods of Analysis. Modern Trends and Applications” Vol. II. Pp. 383-387.

Chalkidiki, 19-22 September 1999. Α39 Determination of lead, cadmium and mercury in surface sediments and mussels.

G.A.Zachariadis, A.N.Anthemidis, I.Caniou, J.A.Stratis. 3rd Euroconference on “Environmental Analytical Chemistry for the 21st Century”

Chalkidiki, 9-15 October 1999. Α40 Spectrophotometric determination of phenols and cyanides after distillation from

natural waters. M.K.Sofoniou, G.A.Zachariadis, A.N.Anthemidis, Th.A. Kouimtzis.

3rd Euroconference on “Environmental Analytical Chemistry for the 21st Century” Chalkidiki, 9-15 October 1999. Α41 Gas chromatographic determination of organochlorine pesticides and

polychlorinated biphenyls in marine sediments and seawater. D.Siakavaras, G.A.Zachariadis, C. Oxinos and J.A. Stratis 2nd Balkan Conference on “Industrial Pollution.” Sofia, 19-21 November 1999.

Α42 πγθξηηηθή κειέηε αλόξγαλσλ ρεκηθώλ ηξνπνπνηεηώλ ζηελ ειεθηξνζεξκηθή αηνκνπνίεζε ηνπ θαδκίνπ κε ηε κέζνδν ζηηο θαζκαηνκεηξίαο αηνκηθήο απνξξόθεζεο (ΔΣAAS). Γ.Α. Εαταρηάδες, Κ.. Σζίθλας, Α.Ν. Αλζεκίδες, Η.Α. ηράηες Ππακηικά 18oς Πανελλήνιος ςνέδπιος Υημείαρ. ελ. 545-548. Πεηραηάς 10-13 Μαρηίοσ 2001.

Α43 Μειέηε ζηηο ειεθηξνζεξκηθήο αηνκνπνίεζεο θαηά ηνλ πξνζδηνξηζκό θαζζηηέξνπ παξνπζία ρεκηθώλ ηξνπνπνηεηώλ κε θαζκαηνκεηξία αηνκηθήο απνξξόθεζεο (ΔΣAAS) Γ.Α. Εαταρηάδες, Μ.Ν. Υαιάηζε, Α.Ν. Αλζεκίδες, Η.Α. ηράηες Ππακηικά 18oς Πανελλήνιος ςνέδπιος Υημείαρ. ελ. 369-372. Πεηραηάς 10-13 Μαρηίοσ 2001.

Α44 Μειέηε ηνπ PTFE σο πξνζξνθεηηθνύ ζε επί ζηηο γξακκήο (on-line) ζπζηήκαηα πξνζπγθέληξσζεο γηα ηνλ πξνζδηνξηζκό κεηάιισλ κε θαζκαηνκεηξία αηνκηθήο απνξξόθεζεο θαη αηνκνπνίεζε κε θιόγα. Α.Ν. Αλζεκίδες, Γ.Α. Εαταρηάδες, Η.Α. ηράηες Ππακηικά 18oς Πανελλήνιος ςνέδπιος Υημείαρ. ελ. 337 – 340. Πεηραηάς 10-13 Μαρηίοσ 2001.

Α45 Flame atomic absorption spectrometric determination of chromium (VI) by on-line on-line preconcentration system using a PTFE packed column.

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A.N. Anthemidis, G.A. Zachariadis, J. Kougoulis, J.A. Stratis Abstracts of the 2nd Intern. Confer. On Instrumental Methods of Analysis, IMA 2001, p. 91. Ioannina, 5-8 September 2001.

Α46 Application of polyurethane foam as a sorbent material for on-line preconcentration of metals in flame atomic absorption spectrometry. A.N. Anthemidis, G.A. Zachariadis, J.A. Stratis Abstracts of the 2nd Intern. Confer. On Instrumental Methods of Analysis, IMA 2001, p. 92. Ioannina, 5-8 September 2001.

Α47 On-line solid phase extraction using PTFE turnings as sorbent material: determination of lead by flame atomic absorption. G.A. Zachariadis, A.N. Anthemidis, P. Bettas, J.A. Stratis Abstracts of the 2nd Intern. Confer. On Instrumental Methods of Analysis, IMA 2001, p. 93. Ioannina, 5-8 September 2001.

Α48 Πξνζδηνξηζκόο κνιύβδνπ θαη θαδκίνπ κε θαζκαηνκεηξία αηνκηθήο απνξξόθεζεο ειεθηξνζεξκηθή αηνκνπνίεζε. Υεκεηνκεηξία ζε πεξηβαι. Γείγκαηα (environmetrics) Γ.Α. Εαταρηάδες, Α.Ν. Αλζεκίδες, Η.Α. ηράηες Ππακηικά 1oς Πεπιβαλλονηικού ςνέδπιος Μακεδονίαρ. ελ. 340-345. Θεζζαιολίθε 1-4 Μαρηίοσ 2002.

Α49 Γηαθπκάλζεηο ησλ ζπγθεληξώζεσλ βαξέσλ θαη ηνμηθώλ ζηνηρείσλ ζηα επηθαλεηαθά λεξά ζηηο Μαθεδνλίαο. Α.Ν. Αλζεκίδες, Γ.Α. Εαταρηάδες, Γ. Βοσηζά, Α. Κούρας, Κ. ακαρά. Ππακηικά 1oς Πεπιβαλλονηικού ςνέδπιος Μακεδονίαρ. ελ. 104-109. Θεζζαιολίθε 1-4 Μαρηίοσ 2002.

Α50 Cloning, expression and purification of the mitochondrial assembly protein Sco2, involved in the pathogenesis of fatal infantile cardioencephalopathy. P.F. Foltopoulou, G. Zachariadis, A.S. Tsiftsoglou, L.C. Papadopoulou. Proceedings of the 2nd Panhellenic Conference in Pharmacology, Επιθεώπηζη Κλινικήρ Φαρκαθοιογίας θαη Φαρκαθοθηλεηηθής, Intern. Edition, 2002, 16, 66.

Αζήλα 2-6 Φεβροσαρίοσ 2002. Α51 Ultra trace on-line preconcentration and determination of cobalt using a PTFE

turnings packed column by electrothermal atomic absorption spectrometry. Aristidis N. Anthemidis, George A. Zachariadis, John A. Stratis Proceedings of “3rd Aegean Analytical Days (3rd AACD)”.

Lesbos, Polichnitos, 29 September – 3 October 2002. Α52 Comparative study of decomposition procedures applied on a ceramic standard

reference material by inductively coupled plasma atomic emission spectroscopy D.N. Papadopoulou, G.A. Zachariadis, A.Ν. Anthemidis, N. Tsirliganis, J. A. Stratis

Proceedings of “3rd Aegean Analytical Days (3rd AACD)”. Lesbos, Polichnitos, 29 September – 3 October 2002. Α53 The implication of the isolated glycoconjugates from mastic gum pistacia lentiscus

on the inhibition of the non-opsonic effect of helicobacter pylori mediated by h.p.neutrophil activated protein (HPNAP). Ph. Kottakis, C. Matragou, G. Zachariadis, M. Weber, T. Choli-Papadopoulou. Proceedings of the 14th Biennial Meeting on Methods of Protein Structure Analysis of the Intern. Association for Protein Structure Analysis and Proteomics. Valencia Spain, September 2002. Proceedings of the 54th Meeting of Hellenic Society of Biochemistry and Molecular Biology, Ioannina, 2002, Vol. 49, p. 164 – 168.

Α54 H εκπινθή ησλ απνκνλσκέλσλ γιπθνπξσηετλώλ ζηηο καζηίραο ζηηο Υίνπ ζηελ παξεκπόδηζε ζηηο νςσληλνπνίεζεο πνπ πξνθαιείηαη από ην Η. Pylori κέζσ ζηηο ΗΡΝΑΡ πξσηετλεο. Φ. Κωηηάθες, Υ. Μαηραγθού, M. Weber, Γ. Εαταρηάδες, Θ. Υοιή-Παπαδοπούιοσ. Πραθηηθά 19οσ Παλειιελίοσ σλεδρίοσ Υεκείας ζει. 404-407. Ζράθιεηο Κρήηες, 6-10 Νοεκβρίοσ 2002.

Α55 A novel ptfe packed column-gas liquid separator (PCGLS) for mercury on-line preconcentration and determination by cold 31roced atomic absorption spectrometry

32

G. A. Zachariadis, A. N. Anthemidis, M. Karpouzi, J. A. Stratis Proceedings of the “3nd International Conference on Instrumental Methods of Analysis, IMA 2003, Thessaloniki, 23-27 September 2003. p. 7-10.

Α56 Study of archaic copper jewels with atomic spectroscopy techniques (ICP-AES, FAAS, SEM-EDS). 2006. N. Anthemidis, G. A. Zachariadis, E. Fragi, E. Mirtsou and J. Stratis Proceedings of the “3nd International Conference on Instrumental Methods of Analysis, IMA 2003, Thessaloniki, 23-27 September 2003. p.425-428.

Α57 Determination of heavy metals in honey and sugars using inductively coupled plasma atomic emission spectrometry. M. D. Ioannidou, G. A. Zachariadis, A. N. Anthemidis, J. A. Stratis Proceedings of the “3nd International Conference on Instrumental Methods of Analysis, IMA 2003, Thessaloniki, 23-27 September 2003. p. 429-432.

Α58 Comparison of ICP-AES and κ-XRF spectrometry for the analysis of ancient ceramics D.N. Papadopoulou, G.A. Zachariadis, A.N. Anthemidis, N.C. Tsirliganis, J.A. Stratis Proceedings of the “3nd International Conference on Instrumental Methods of Analysis, IMA 2003, Thessaloniki, 23-27 September, 2003. p. 433-436.

Α59 On-line sample preconcentration /separation by flow injection liquid-liquid extraction coupled to electrothermal atomic absorption spectrometry as demonstrated for ultra-trace cadmium determination in environmental and biological samples ETAAS. N. Anthemidis, G. A. Zachariadis, J. A. Stratis Proceedings of the “3nd International Conference on Instrumental Methods of Analysis, IMA 2003, Thessaloniki, 23-27 September, 2003. p. 60-63.

Α60 Flow injection on-line liquid liquid extraction using a gravitational phase separator for flame atomic absorption spectrometry. N. Anthemidis, G. A. Zachariadis, C. Farastelis, J. A. Stratis Proceedings of the “3nd International Conference on Instrumental Methods of Analysis, IMA 2003, Thessaloniki, 23-27 September, 2003. p. 234-237.

Α61 Micro-XRF analysis of ancient ceramics that exhibit color variations in their lateral cross-section. D. Papadopoulou, G. A. Zachariadis, A. N. Anthemidis, N. Tsirliganis, J. A. Stratis Proceedings of the 34th International Symposium on Archaeometry “Archaeometry 2004”, Saragosa, Spain, 3-7 May 2004, p.260.

Α62 On-line speciation of mercury and methylmercury by cold vapour atomic absorption spectrometry using selective solid phase extraction. G. Zachariadis, A. Anthemidis, E. Daftsis, J. Stratis Proceedings of the “4th Aegean Analytical Chemistry Days (4th AACD)” Kusadasi-Aydin, Turkey, 29 September – 3 October, 2004. pp. 408-410.

Α63 Effect of Different Conventional Digestion Techniques on the determination of Essential, Non-Essential and Toxic Trace Elements in Cereals and Cereal flours by Inductively Coupled Plasma-Atomic Emission Spectrometry Adballa Momen, G. Zachariadis, A. Anthemidis, J. Stratis Proceedings of the “4th Aegean Analytical Chemistry Days (4th AACD)” Kusadasi-Aydin, Turkey, 29 September – 3 October, 2004. pp. 515-517.

Α64 Αλάπηπμε κεζόδνπ πξνζδηνξηζκνύ κεζπιπδξαξγύξνπ ζε νξό αίκαηνο κε θαζκαηνκεηξία αηνκηθήο απνξξόθεζεο ςπρξνύ αηκνύ θαη εθιεθηηθό δηαρσξηζκό ζε ζηήιε κε ptfe σο πιεξσηηθό πιηθό. Δ. Η. Γάθηζες, Γ. Α. Εαταρηάδες, Α. Ν. Αλζεκίδες, θαη Η. Α. ηράηες Ππακηικά 8ος ςνέδπιος Υημείαρ Ελλάδαρ Κύππος. Θεζζαιολίθε, 10-13 Γεθεκβρίοσ 2004. ζει. 113.

Α65 Αλάπηπμε κεζόδσλ δηαιπηνπνίεζεο πξόηππνπ δείγκαηνο γπαιηνύ SRM 621 κε επίδξαζε κηθξνθπκάησλ θαη πξνζδηνξηζκόο κεηάιισλ κε ηελ ηερληθή ζηηο θαζκαηνκεηξίαο αηνκηθήο εθπνκπήο επαγσγηθά ζπδεπγκέλνπ πιάζκαηνο (ICPAES) Δ. Γεκεηραθούδε, Γ. Εαταρηάδες, Α. Αλζεκίδες, Η. ηράηες Ππακηικά 8ος ςνέδπιος Υημείαρ Ελλάδαρ Κύππος Θεζζαιολίθε, 10-13 Γεθεκβρίοσ 2004. ζει. 114.

33

Α66 Αλάπηπμε on-line ζπζηήκαηνο δηαδνρηθήο έγρπζεο γηα ηνλ πξνζδηνξηζκό ηνπ αξζεληθνύ κε ηερληθή παξαγσγή πδξηδίσλ θαη θαζκαηνκεηξία αηνκηθήο απνξξόθεζεο Α. Ν. Αλζεκίδες, Γ. Α. Εαταρηάδες, Γ. Σζηκατίδες, Η. Α. ηράηες Ππακηικά 8ος ςνέδπιος Υημείαρ Ελλάδαρ Κύππος Θεζζαιολίθε, 10-13 Γεθεκβρίοσ 2004. ζει. 112.

Α67 Οptimized method for multi-element determination of metals in antitussives by direct syrup dilution technique and inductively coupled plasma atomic emission spectrometry. G.A. Zachariadis, D. Kapsimali Book of Abstracts of the 4th International Conference on Instrumental Methods of Analysis, IMA 2005, Iraklion Crete, 2-6 October 2005. ζει. 368.

Α68 Determination of toxic metals in antibiotics by using inductively coupled plasma atomic emission spectrometry with various spray-chamber and nebulizer configurations and the slurry technique G.A. Zachariadis, C.E. Michos, A.N. Anthemidis Book of Abstracts of the 4th International Conference on Instrumental Methods of Analysis, IMA 2005, Iraklion Crete, 2-6 October 2005. ζει. 465.

Α69 Evaluation of the standard addition technique in wet acid digestion of certified fish reference material for multi-element determination by inductively coupled plasma atomic emission spectrometry G. A. Zachariadis, N.M. Tzollas, A.N. Anthemidis, J.A. Stratis Book of Abstracts of the 4th International Conference on Instrumental Methods of Analysis, IMA 2005, Iraklion Crete, 2-6 October 2005. ζει. 367.

Α70 Development and validation of various digestion procedures for multi-element determination of toxic and nutrient contents in legume samples by ICP-AES A.A. Momen, G.A. Zachariadis, A.N. Anthemidis, J.A. Stratis Book of Abstracts of the 4th International Conference on Instrumental Methods of Analysis, IMA 2005, Iraklion Crete, 2-6 October 2005. ζει. 466.

Α71 Determination of mercury and methyl mercury in human blood fractions after centrifugal separations and cold vapour atomic absorption spectrometry E.I. Daftsis, G.A. Zachariadis, A.N. Anthemidis Book of Abstracts of the 4th International Conference on Instrumental Methods of Analysis, IMA 2005, Iraklion Crete, 2-6 October 2005. ζει. 374.

Α72 Multi-element determination of Cu, Mn, Ni, Cr, Co, Cd and Pb in human blood fractions by ICP-AES or ETAAS

E.I. Daftsis, G.A. Zachariadis Book of Abstracts of the 4th International Conference on Instrumental Methods of Analysis, IMA 2005, Iraklion Crete, 2-6 October 2005. ζει. 375.

Α73 Optimization and comparison of two digestion methods for multi-element analysis of plant material By ICP-AES A. Momen, G. A. Zachariadis, A. N. Anthemidis, J. A. Stratis Book of abstracts of the “5th Aegean Analytical Chemistry Days (5th AACD)” Thessaloniki, Greece, 5-8 October, 2006. p. 18.

Α74 Development of multielement analysis of multivitamin preparations by Inductively coupled plasma optical emission spectrometry slyrry/wet digestion sample introduction Ag. Olympiou, G. A. Zachariadis Book of abstracts of the “5th Aegean Analytical Chemistry Days (5th AACD)” Thessaloniki, Greece, 5-8 October, 2006. p. 108.

Α75 Development of multielement methods for the analysis of powdered infant milk by direct suspension introduction or after wet digestion and Inductively Coupled Plasma Atomic Emission Spectrometry L. Valianou, G.A. Zachariadis Book of abstracts of the “5th Aegean Analytical Chemistry Days (5th AACD)” Thessaloniki, Greece, 5-8 October, 2006. p. 109.

Α76 Determination of toxic metals in human blood and blood fractions by atomic

34

spectrometry techniques E. Daftsis, G. Zachariadis Book of abstracts of the “5th Aegean Analytical Chemistry Days (5th AACD)” Thessaloniki, Greece, 5-8 October, 2006. p. 177.

Α77 Application of micro-X-ray spectroscopy to the study of black glazed ancient ceramics. D. Papadopoulou, G. A. Zachariadis, A. Anthemidis, N. Tsirliganis, J. Stratis Book of abstracts of the “5th Aegean Analytical Chemistry Days (5th AACD)” Thessaloniki, Greece, 5-8 October, 2006. p. 178

Α78 Head-space solid phase-microextraction of butyl- and phenyltin compounds in human urine after derivatisation with sodium tetraethylborate and subsequent determination by capillary gas chromatography with microwave-induced plasma atomic emission and mass spectrometric detectors. G. A. Zachariadis, E. Rosenberg, Book of abstracts of the 11th Workshop on Progress of Analytical Methodologies for Trace Metal Speciation (TraceSpec 2007)”. Munster, Germany, 4-7 September 2007. p. 121

Α79 Determination of organotin compounds in urine and aqueous samples by GC-MIP-AED and GC-MS after ethylation and liquid-liquid extraction G. A. Zachariadis, E. Rosenberg, Book of abstracts of 5th Intern. Confer. On Instrumental Methods of Analysis, IMA 2007”. Rio, Patras, Greece, 30 Sept – 3 October 2007. p113.

Α80 Gas chromatographic mass spectrometric determination of organomercury species G. A. Zachariadis, D. Kapsimali, Book of abstracts of 5th Intern. Confer. On Instrumental Methods of Analysis, IMA 2007”. Rio, Patras, Greece, 30 Sept – 3 October 2007. p112.

Α81 Comparison and evaluation of the standard methods ion chromatography and spectrophotometry UV-VIS for the determination of ammonia in geothermal water samples N. M. Tzollas, G. A. Zachariadis, A. N. Anthemidis. J. A. Stratis Book of abstracts of 5th Intern. Confer. On Instrumental Methods of Analysis, IMA 2007”. Rio, Patras, Greece, 30 Sept – 3 October 2007. p240.

Α82 Comparison of liquid-liquid- and head-space solid phase-microextraction for the speciation analysis of butyl- and phenyltin compounds in human urine by tetraalyklborate derivatisation and GC/AED and GC/MS determination Erwin Rosenberg and George A. Zachariadis 32nd International Symposium on Capillary Chromatography and 5th GCxGC Symposium Riva del Garda, Italy, 26-30 May 2008. Book of abstracts C12.

Α83 Effect of sample matrix on the sensitivity of fast mercury and methylmercury quantitation in human urine, saliva and serum by gas chromatography mass spectrometry, after aqueous alkylation and headspace solid phase microextraction. G. A. Zachariadis, D. Kapsimali, Book of abstracts of 14th Biennial National Atomic Spectroscopy Symposium, 14th BNASS” Brighton, Sussex, United Kingdom, 7 – 9 July 2008. p. 212.

Α84 Signal normalization in axial-viewing inductively coupled plasma atomic emission spectrometry using yttrium as internal standard and certified reference materials G. A. Zachariadis, C. Sarafidou, Book of abstracts of 14th Biennial National Atomic Spectroscopy Symposium, 14th BNASS” Brighton, Sussex, United Kingdom, 7 – 9 July 2008. p. 210

Α85 Flow injection on-line metal preconcentration using a PTFE-turnings packed column for nickel and zinc determination by flame atomic absorption spectrometry. A. Anthemidis, G. A. Zachariadis, J. A. Stratis Book of abstracts of the “6th Aegean Analytical Chemistry Days (6th AACD)” Denizli, Turkey, 9-12 October, 2008. p. 131.

Α86 Determination of ammonium in high mineral content water samples and geothermal waters N. M. Tzollas, G. A. Zachariadis, A. N. Anthemidis, J. A. Stratis

35

Book of abstracts of the “6th Aegean Analytical Chemistry Days (6th AACD)” Denizli, Turkey, 9-12 October, 2008. p. 411.

Α87 Modern and Hyphenated Analytical Techniques for Metal Analysis of Environmental, Food, Pharmaceutical and Biological Matrices G. A. Zachariadis Proceedings of the 5th EEESD 09, Vouliagmeni, 28-30 September 2009, p. 60-63.

Α88 Speciation of tetralkyltin compounds by headspace solid-phase microextraction and gas chromatography – mass spectrometry N. M. Tzollas, G. A. Zachariadis Book of abstracts of 6th Intern. Confer. On Instrumental Methods of Analysis, IMA 2009”. Athens, Greece, 4 – 8 October 2009. p. 197.

Α89 Fast method for organolead and organotin speciation in urine by hyphenated GC-MIP-AED after LLE extraction George A. Zachariadis and Erwin Rosenberg Book of abstracts of 6th Intern. Confer. On Instrumental Methods of Analysis, IMA 2009”. Athens, Greece, 4 – 8 October 2009. p. 106.

Α90 Tetraethylborate and tetraphenylborate as derivatizing agents for selenite determination in human urine by GC-MS after headspace solid phase microextraction D. Kapsimali, G. A. Zachariadis Book of abstracts of 6th Intern. Confer. on Instrumental Methods of Analysis, IMA 2009”. Athens, Greece, 4 – 8 October 2009. p. 105.

Α91 Introduction of biological, food and pharmaceutical samples to Inductively Coupled Plasma Atomic Emission Spectrometry (ICP-AES) G. A. Zachariadis Book of abstracts of 6th Intern. Confer. On Instrumental Methods of Analysis, IMA 2009”. Athens, Greece, 4 – 8 October 2009. p. 79.

Α92 Speciation of organotin compounds by Liquid Chromatography and an Ion trap-Time of Flight mass spectrometric detector N. M. Tzollas, G. A. Zachariadis, E. Rosenberg Book of abstracts of 6th Intern. Confer. On Instrumental Methods of Analysis, IMA 2009”. Athens, Greece, 4 – 8 October 2009. p. 68.

Α93 Peek-turnings, a novel sorbent material for trace metal determination by flow injection flame atomic absorption spectrometry. Multivariable optimization by a two-level full factorial design A.N. Anthemidis, I.S.I. Adam, G.A. Zachariadis Book of abstracts of 6th Intern. Confer. on Instrumental Methods of Analysis, IMA 2009”. Athens, Greece, 4 – 8 October 2009. p. 104.

Α94 Microwave-assisted acid extraction methodology for trace elements in mastic gum using inductively coupled plasma atomic emission spectrometry G.A. Zachariadis, E. Spanou Book of abstracts of 14th Hellenic Medicinal Chemistry Symposium”. Thessaloniki, Greece, 23 –25 April 2010. p. 147.

Α95 Titanium and trace elements in drugs and cosmetics by ICP AES G.A. Zachariadis, E. Sahanidou Book of abstracts of 14th Hellenic Medicinal Chemistry Symposium”. Thessaloniki, Greece, 23 –25 April 2010. p. 148.

Α96 Analytical methodology for the determination of a-pinene, b-myrcene and p-cymene in human urine by headspace SPME and gas chromatography mass spectrometry G.A. Zachariadis, A. Ntantasios Book of abstracts of 14th Hellenic Medicinal Chemistry Symposium”. Thessaloniki, Greece, 23 –25 April 2010. p. 153.

Α97 Development of analytical method for determination of volatile compounds in mastic Gum extracts and mastic oil by solid phase microextraction and gas chromatography mass spectrometry G.A. Zachariadis, A. Langioli

36

Book of abstracts of 7th AACD Aegean Analytical Chemistry Days, Lesvos, Greece, 29 Sept –3 Octob 2010. p. 131.

Α98 Investigation of the HPLC-IT-TOF-MS technique with atmospheric pressure chemical ionization for the speciation of selenocystine, selenomethionine, selenoethionine, dimethyldiselenide and diphenyldiselenide D. Kapsimali, E. Rosenberg, G.A. Zachariadis. Book of abstracts of 7th AACD Aegean Analytical Chemistry Days, Lesvos, Greece, 29 Sept –3 Octob 2010. p. 134.

Α99 Storage stability studies of tributyltin in human urine samples by the use of headspace spme technique and GC-MS G.A. Zachariadis, M. Nikolaou, N. Tzollas, E. Rosenberg Book of abstracts of 7th AACD Aegean Analytical Chemistry Days, Lesvos, Greece, 29 Sept –3 Octob 2010. p. 217.

Α100 An evaluation of the use of yttrium internal standard in icpaes concerning untreated aqueous solutions in presence of high concentration of organic solvents and matrices. G.A. Zachariadis, C. Vogiatzis Book of abstracts of 7th AACD Aegean Analytical Chemistry Days, Lesvos, Greece, 29 Sept –3 Octob 2010. p. 36.

Α101 Investigation of organomercury species stability in human urine samples under various storage conditions using headspace SPME GC-MS. G.A. Zachariadis, D. Ntontou Book of abstracts of 7th AACD Aegean Analytical Chemistry Days, Lesvos, Greece, 29 Sept –3 Octob 2010. p. 132.

Α102 Speciation method for Cr3+, Cr6+, As3+ / As5+ determination by solid phase extraction and inductively coupled plasma atomic emission spectrometry. G.A. Zachariadis, E.Trikas Book of abstracts of 36th International Conference of Environmental Analytical Chemistry”. Rome, Italy, 5 –9 October 2010. p.47.

A103 Recent developments in High Resolution Tandem Mass Spectrometry for biomolecules and related analytes G.A. Zachariadis, 2nd International Conference on Biochemistry and Medical Chemistry 2nd BIOMEDCH11, University of Cambridge, 23 – 25 February 2011. p.375-378.

Α104 Use of Doehlert-type designs in the optimization of a hybrid Ion Trap TOF-MS technique for glutathione determination by Electrospray Ionization. G.A. Zachariadis, E.Rosenberg IMA 2011, Chania, Crete, 18-22 September 2011, pp.11.

Α105 A novel non-destructive spot desorption technique for archaeometric analysis of essential oils residues using headspace solid phase microextraction and capillary GC-MS G.A. Zachariadis, L. Valianou IMA 2011, Chania, Crete, 18-22 September 2011, pp.171.

A106 Speciation Analysis of Health-Related Small-Molecular Weight Organometallic Compounds by LC-IT-TOF-MS E. Fernandez-Diez, G.A. Zachariadis and E. Rosenberg IMA 2011, Chania, Crete, 18-22 September 2011, pp.233.

Α107 Photocatalytic degradation of the anticancer drug Carboplatin and recovery of Pt, V. Kitsiou, I. Poulios, G. A. Zachariadis 7th European meeting on Solar chemistry and photocatalysis, Porto, June, 17-20, 2012.

Α108 A new method for simultaneous determination of metal and non-metal anions based on the on-line coupling of ICP-AES to ion chromatography without signal suppression G.A. Zachariadis, E. Spanou. Book of abstracts of 12th Flow Analysis Conference”. Thessaloniki, Greece, 23 –28 September 2011. p. 54.

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Α109 Recent applications concerning FIA of metal-containing biomolecules (metallomics) C. Vogiatzis, G.A. Zachariadis. Book of abstracts of 12th Flow Analysis Conference”. Thessaloniki, Greece, 23 –28 September 2011. p. 81.

Α110 Study of carboplatin interactions with DNA nucleosides using LCMS-IT-TOF. C. Vogiatzis, E. Rosenberg, G.A. Zachariadis. Book of abstracts of 12th Flow Analysis Conference”. Thessaloniki, Greece, 23 –28 September 2011. p. 82.

Α111 A fast method for platinum determination in carboplatin containing biofluids from anticancer treated patients by direct ICP-AES and on-line preconcentration SPE-ICP- AES. G.A. Zachariadis, C. Karageorgaki. Book of abstracts of 12th Flow Analysis Conference”. Thessaloniki, Greece, 23 –28 September 2011. p. 107.

25. ARTICLES PUBLISHED IN PERIODICALS

Copies of these publications are given in the CD, Part IX, Chapter 25. Σ1 Analysing the Past: Archaeological Chemistry.

G. Zachariadis. Pemptousia (periodical), Issue 21, 2006.

Σ2 Heavy Metals: Health threat. G. Zachariadis. Pemptousia (periodical), Issue 24, 2007.

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26. IMPACT FACTORS OF JOURNALS

Impact factors of 2011 as published in 2012 from Web of Science (Journal Citation Ranking). I was Corresponding author in 37 papers, which I published as Associate or Assistant professor.

Paper number

(*)

Number of

pages

Number of co- authors

JOURNAL TITLE Year of

publ.

Impact Factor 2011

a

Impact Factor 5-years

Cita-

tions f

Γ01 5 3 Fresenius Journal of Analytical Chemistry b 1988 3.778 3.733 6

Γ02 6 2 Toxicological and Environmental Chemistry a 1989 - 1

Γ03 10 4 J Environmental Science and Health A 1990 1.190 1.344 0

Γ04 13 4 J Environmental Science and Health A 1991 1.190 1.344 1

Γ05 6 2 Science of the Total Environment 1991 3.286 3.536 5

Γ06 7 1 Journal of Analytical Atomic Spectrometry 1991 3.220 2.966 25

Γ07 7 3 Fresenius Environmental Bulletin 1993 0.660 0.635 22

Γ08 6 3 J Environmental Science and Health A 1993 1.190 1.344 0

Γ09 7 2 Journal of Liquid Chromatography 1993 0.706 0.884 11

Γ10 14 4 Journal of Liquid Chromatography 1994 0.706 0.884 16

Γ11 12 4 Journal of Liquid Chromatography 1994 0.706 0.884 5

Γ12 14 3 Environmental Education and Information 1994 - 1

Γ13 12 3 J Environmental Science and Health A 1994 1.190 1.344 12

Γ14 13 3 J Environmental Science and Health A 1995 1.190 1.344 9

Γ15 4 5 Journal of Analytical Atomic Spectroscopy 1995 3.220 2.966 44

Γ16 7 3 Archieves of Envir. Contam. And Toxicology 1995 1.927 2.102 54

Γ17 2 4 Fresenius Journal of Analytical Chemistry b 1995 3.778 3.733 3

Γ18 3 2 Fresenius Journal of Analytical Chemistry b 1995 3.778 3.733 0

Γ19 9 4 Journal of Liquid Chromatography 1995 0.706 0.884 5

Γ20 6 2 Analyst 1995 4.230 4.119 18

Γ21 10 4 Toxicological and Environmental Chemistry a 1995 - 17

Γ22 8 3 Ecotoxicology and Environmental Safety 1995 2.294 2.617 51

Γ23 8 3 Microchimica Acta 1995 3.033 2.508 10

Γ24 9 4 Toxicological and Environmental Chemistry 1996 - 2

Γ25 6 5 Fresenius Journal of Analytical Chemistry b 1996 3.778 3.733 1

Γ26 15 3 J Environmental Science and Health A 1996 1.190 1.344 9

Γ27 6 4 Fresenius Environmental Bulletin 1996 0.660 0.635 2

Γ28 4 3 Εnvironmental and Experimental Botany 1997 2.985 3.644 24

Γ29 14 6 Instrumentation Science and Technology 1997 0.430 0.386 3

Γ30 7 3 Talanta 1998 3.794 3.747 9

Γ31 4 3 Journal of Analytical Atomic Spectroscopy 1998 3.220 2.966 61

Γ32 8 4 Toxicological and Environmental Chemistry 1999 - 4

Γ33 10 4 Toxicological and Environmental Chemistry 1999 - 0

Γ34 5 2 Environmental Pollution 1999 3.746 3.987 23

Γ35 8 2 Bulletin of Environ. Contam. And Toxicology 1999 1.018 1.050 16

Γ36 13 3 Intern. J. of Environ. Analytical Chemistry 2000 1.162 1.163 0

Γ37 5 3 Journal of Environ. Protection and Ecology 2000 0.102 0

Γ38 8 3 Microchimica Acta 2001 3.033 2.508 3

Γ39 14 3 Intern. J. of Environ. Analytical Chemistry 2001 1.162 1.163 4

Γ40 13 4 Ecotoxicology and Environmental Safety 2001 2.294 2.617 41

39

(continued)

Paper number

(*)

Number of

pages

Number of co- authors

JOURNAL TITLE Year of

publ.

Impact Factor 2011*

Impact Factor 5-years

Cita-

tions b

Γ41 8 2 Talanta 2001 3.794 3.747 72

Γ42 5 3 Εnvironment International 2001 5.297 5.339 37

Γ43 8 3 Bulletin of Environ. Contam. And Toxicology 2001 1.018 1.050 58

Γ44 11 3 J. Biomedical Pharmaceutical Analysis 2002 2.967 2.979 7

Γ45 8 3 Σalanta 2002 3.794 3.747 49

Γ46 13 3 Water, Air and Soil Pollution 2002 1.625 1.892 16

Γ47 9 3 Σalanta 2002 3.794 3.747 42

Γ48 6 8 Fresenius Environmental Bulletin 2002 0.660 0.635 1

Γ49 10 2 Σalanta 2002 3.794 3.747 72

Γ50 5 2 Journal of Analytical Atomic Spectrometry 2002 3.220 2.966 24

Γ51 9 2 Journal of Analytical Atomic Spectrometry 2003 3.220 2.966 2

Γ52 8 2 Σalanta 2003 3.794 3.747 24

Γ53* 5 3 Journal of Analytical Atomic Spectrometry 2003 3.220 2.966 10

Γ54 4 2 Journal of Analytical Atomic Spectrometry 2003 3.220 2.966 48

Γ55 6 7 Water Research 2003 4.865 5.315 231

Γ56 5 6 Fresenius Εnvironmentl Bulletin 2003 0.660 0.635 1

Γ57 7 3 Σalanta 2004 3.794 3.747 74

Γ58 9 4 Analytica Chimica Acta 2004 4.555 4.144 16

Γ59 12 4 Molecular Genetics and Metabolism 2004 3.193 3.080 15

Γ60 6 3 Analytical and Bioanalytical Chemistry 2004 3.778 3.733 26

Γ61 5 2 Σalanta 2004 3.794 3.747 18

Γ62* 6 3 Σalanta 2005 3.794 3.747 35

Γ63 8 4 Spectrochimica Acta Part B 2004 2.876 2.740 20

Γ64 6 2 Analytica Chimica Acta c 2005 4.555 4.144 18

Γ65* 3 3 Journal of Analytical Atomic Spectrometry 2005 3.220 2.966 23

Γ66* 15 3 Journal of AOAC International 2005 1.199 1.359 2

Γ67* 9 3 Σalanta 2006 3.794 3.747 3

Γ68 7 4 Σalanta 2006 3.794 3.747 23

Γ69* 8 3 Analytica Chimica Acta 2006 4.555 4.144 9

Γ70* 7 1 J. Biomedical and Pharmaceutical Analysis 2006 2.967 2.979 2

Γ71* 8 3 Σalanta 2007 3.794 3.747 12

Γ72* 8 1 Σalanta 2007 3.794 3.747 16

Γ73* 8 1 J. Biomedical Pharmaceutical Analysis 2007 2.967 2.979 4

Γ74* 7 1 Microchimica Acta 2008 3.033 2.508 5

Γ75* 7 3 Microchimica Acta 2008 3.033 2.508 6

Γ76 8 5 Aminoacids 2008 3.248 3.138 7

Γ77* 5 1 Applied Spectroscopy 2008 1.663 1.724 3

Γ78* 6 1 J. Pharmaceutical and Biomedical Analysis 2008 2.967 2.979 6

Γ79* 9 1 J. Separation Science 2008 2.733 2.725 5

Γ80* 7 1 Talanta 2009 3.794 3.747 14

(continued)

40

(continued)

Paper number

(*)

Number of

pages

Number of co- authors

JOURNAL TITLE Year of

publ.

Impact Factor 2011

a

Impact Factor 5-years

Cita-

tions b

Γ81* 5 1 J. Chromatography B 2009 2.888 3.057 10

Γ82* 5 1 Microchimica Acta 2009 3.033 2.508 4

Γ83* 7 1 J. Pharmaceutical Biomedical Analysis d 2009 2.967 2.979 10

Γ84* 4 1 J. Chromatography B 2009 2.888 3.057 5

Γ85 11 2 Intern. J. of Environ. Analytical Chemistry 2010 1.162 1.163 8

Γ86* 7 1 Talanta 2010 3.794 3.747 6

Γ87* 11 3 Intern. J. of Environ. Analytical Chemistry 2010 1.162 1.163 0

Γ88* 4 1 J. Separation Science 2010 2.733 2.725 1

Γ89 5 2 Talanta 2010 3.794 3.747 11

Γ90* 18 2 Current Organic Chemistry 2010 3.064 3.468 1

Γ91* 19 1 Applied Spectroscopy Reviews 2010 3.385 4.475 0

Γ92* 5 1 Phytochemical Analysis 2010 2.633 2.320 1

Γ93* 5 1 Central European J Chemistry 2011 1.073 1.035 1

Γ94 5 2 Environmental and Experimental Botany 2011 2.985 3.644 0

Γ95* 9 1 Journal of Anal. Atomic Spectrometry 2011 3.220 2.966 0

Γ96* 7 2 Central European J Chemistry 2011 1.073 1.035 0

Γ97 7 2 Analytical Methods 2011 1.547 1.547 1

Γ98 13 10 Investigational New Drugs 2012 3.357 2.946 3

Γ99* 7 1 Intern J Environm. Analyt. Chemistry 2012 1.162 1.163 0

Γ100* 9 2 Analytical Letters 2012 1.016 1.107 0

Γ101* 11 1 Analytical Letters 2012 1.016 1.107 0

Γ102 6 10 Journal of Material Science 2012 2.015 1.918 0

Γ103* 6 1 J Separation Science 2012 2.733 2.725 0

Γ104* 7 3 Biomedical Chromatography 2012 1.966 1.880 0

Γ105* Γ106*

9 3

2 0

Current Analytical Chemistrye

Current Analytical Chemistrye

In Pr In Pr

1.000 1.000

1.337 1.337

0 0

Γ107* 11 1 Rapid Communication Mass Spectrometry f In Pr 2.79 2.782

Total 1636g

Mean 2.70 2.70

Notes: Papers Γ76 to Γ107 were done from the position of Associate Professor (*) In papers with asterisk, I was the Corresponding Author. a The old Toxicological and Environm. Chemistry journal is renewed (Taylor Francis) and it is

again going to have impact factor from year 2013. (See in electronic information from the journal website).

b Fresenius Journal of Analytical Chemistry journal was replaced by Analytical and Bioanalytical Chemistry.

c This paper was in the list of the 25 most downloaded papers of the ANALYTICA CHIMICA ACTA journal.

d The conclusions of this paper were included in the ―Sunscreens‖ article of WIKIPEDIA. e These two articles are in press at the time of the submission of this curriculum. f This article is in press at the time of the submission of this curriculum. g The total citations (excluding the self-citations) is 1636 (until November 1, 2012) and the H-index

23. Except from the 1619 citations found from ―SCOPUS AUTHOR SEARCH‖ there are 17 other, older than 1995, which are not covered by SCOPUS but from Science Citation Reports.

41

27. BIBLIOGRAPHIC STATISTICS FOR MY PAPERS

Some statistics for papers and journal Impact Factors Total number of papers 107

Number of papers as Associate Professor 32

Rate of papers per year, as Associate Professor 32/5 = 6.4

Mean Impact Factor of Journals Year 2011 = 2.70

Mean Impact Factor of Journals 5-years = 2.70

Percentage (%) of papers as Corresp. Author, in all papers (37τ100/107) = 34.6%

Percentage (%) of papers as Corresp. Author, in Associate Prof. (25τ100/32) = 78.1 %

Percentage (%) of papers as First Author, in Associate Prof. (18τ100/32) = 56.2 %

Citations and Indeces Citations (excluding self-ciatations) 1636

H-index 23

G-index 37

FIGURE FROM ISI WEB OF KNOWLEDGE AUTHOR ZACHARIADIS G.A., recorded in November 1, 2012

Figure 1. Citations (excluding self citations) per year

42

PART ΙΙ ANALYSIS OF SCIENTIFIC WORK

Note: Copies of the papers are included in CD, Part III using the code Δ1, Δ2, Δ3, ……. Γ1 Critical comparison of decomposition procedures for atomic absorption

spectrometric analysis of prehistorical ceramics. J. A. Stratis, G. A. Zachariadis, E. A. Dimitrakoudi and V. Simeonov. Fresenius Journal of Analytical Chemistry, 1988, 331, 725-729.

This paper describes an effort to compare acid and fusion decomposition techniques for ceramic samples taken from a Neolithic settlement. An interpretation of the results is given following two statistical ways, the analysis of variance (ANOVA) and rank correlation. Also an ideographic approach is performed. Finally, conclusions are presented about comparability of the above representative digestions. Γ2 Preliminary environmental study of New Karvali bay (Kavala Gulf, North Greece).

K. G. Ouzounis, G. A. Zachariadis and J. A. Stratis. Toxicological and Environmental Chemistry, 1989, 20-21, 227-232.

In this work, the results of an extended environmental study are presented in reference to New Karvali bay, in Kavala, Greece. This region is influenced from the industrial effluents of nearby Fertilizers production industry. Accordingly the concentration levels and time variation of nitrates, phosphates and fluorides were determined and monitored by spectrophotometric techniques. On the other hand the concentration levels of heavy metals were determined by flame AAS after MIBK extraction of the dithiocarbamate complexes in a suitable pH. Γ3 Environmental study on chemical pollution of the lake Koronia in Northern Greece.

G. Vassilikiotis, J. Stratis, A. Voulgaropoulos, G. Zachariadis and V. Michalopoulou. Journal of Environmental Science and Health, 1990, A25, 611-620.

In this paper, an annual environmental study from the chemical point of view is reported, for the Lake Koronia in the major district of Thessaloniki. The survey is based upon determinations of chemical and physicochemical parameters such as pH, conductivity, nutrients, heavy metal concentrations etc. The samples were collected monthly from six sampling stations, and they were surface water and surface sediments. The state of pollution of the lake is discussed and compared with reported values. Γ4 Determination of chemical pollution parameters of river Axios in Northern Greece.

G. Vassilikiotis, A. N.Voulgaropoulos, J. Stratis, V. Michalopoulou and G. A. Zachariadis. Journal of Environmental Science and Health, 1991, A26, 621-633.

The degree of pollution of the River Axios in Northern Greece has been evaluated by determination of certain chemical parameters, such as dissolved oxygen, pH, conductivity, nutrients and heavy metals. The samples were collected monthly, for a period of 1 year, from three sampling stations within the greek section of the river, and they were surface water and surface sediments. In sediments it was observed that the hydrochloric extractable portion of the metals in sediments was similar to the total one. In water samples the variation of metals Pb, Cd, Zn, Mn and Ni were not important. Γ5 Distribution of heavy metals in sediments and aquatic plants of the river Pinios

(Central Greece). T. Sawidis, J. Stratis and G. Zachariadis. Τhe Science of the Total Environment, 1991, 102, 261-266.

Surface sediments and aquatic plants from the River Pinios, Greece, were collected and analyzed for Fe, Mn, Ni, Zn, Cr, and Cu, in order to obtain information about the heavy metal contamination of the river. The selected species proved to be good indicators for the monitoring of heavy metals. The distribution of heavy metals in the root, the stem and the leaves of the plants is also investigated.

43

Γ6 Optimization of cold vapour atomic absorption spectrometric determination of mercury (CVAAS) with and without amalgamation by subsequent use of complete and fractional factorial designs with univariate and modified Simplex methods. G. Zachariadis and J. Stratis. Journal of Analytical Atomic Spectrometry, 1991, 6, 239-245.

Experimental conditions for the determination of Hg with commercially available purpose-built apparatus were optimized by sequential use of two multiple parameter methods, based on a factorial design and a modified simplex procedure. The responses that were evaluated to determine the optimum conditions were the peak height and peak area of the Hg signal. The significance of the effects was tested using the analysis of variance (ANOVA), at a 99% level of significance. Interactions observed between the parameters were quantitatively evaluated and discussed. The flow-rate of air, volume of sample solution, use of a desiccant and the interactions between these parameters in the determination of Hg by cold vapour atomic absorption spectrometry (CVAAS) without amalgamation were optimized according to a complete 23 factorial design and a univariate method. The experimental design was also considered for the determination of Hg after amalgamation on an Au-Pt gauze. The flow-rate of nitrogen, mass of the amalgamator, trapping time, releasing time and interactions between them were statistically evaluated, according to a fractional factorial design (half-replicate of a complete 24 factorial design) and subsequent use of the modified simplex method. This approach for partial optimization of a commercial system is rapid and has many advantages over simple univariate methods. An absolute detection limit of 0.33 ng of Hg was achieved using the amalgamation technique for a total solution volume of 50 ml. This is comparable to the limits obtained by univariate methods of optimization. An approximately 10-fold improvement in the detection limit was observed with this technique in comparison with the direct method. Γ7 Mosses as biological indicators for monitoring of heavy metal pollution.

T. Savidis, G. Zachariadis, J. Stratis and E. Ladoukakis. Fresenius Environmental Bulletin, 1993, 2, 193-199.

The bioaccumulation of lead copper zinc and cadmium in mosses collected from polluted and unpolluted areas is presented in this work. Acid digestion and subsequent use of atomic absorption spectrometry was the analytical methodology followed for the analysis. The concentration of accumulated heavy metals differs in various moss species from the same biotope. The possibility of a good estimation of atmospheric pollution by analysing heavy metal content of mosses is also indicated, by application of this methodology in plants from polluted and unpolluted areas of Greece. The aid of moss analysis in prospecting for melal mineralisation is also discussed. Γ8 Furazolidone determination at random specimens of consumable bovine-pig

muscles by HPLC. I. Caniou, H. Tsoukali, P. Epivatianos and G. Zachariadis. Journal of Environmental Science and Health. 1993, Α28, 645-650.

For the qualitative and quantitative determination of furazolidone, HPLC technique was used. Identification was done through fluorescent derivative by HPTLC. The results of HPLC were used for quantitation after suitable calibration with standard solutions. Sixty pig and 180 bovine samples were analyzed for furazolidone presence. In only 10 specimens furazolidone was detected at a mean concentration of 3.7 κg . kg-1. Γ9 Separation and determination of five penicillins by reversed phase HPLC.

I. P. Caniou, G. A. Zachariadis and J. A. Stratis. Journal of Liquid Chromatography, 1993, 16, 2891-2897.

Rapid separation and quantitative determination of mixtures of amoxicillin, ampicillin, benzylpenicillin, cloxacillin and dicloxacillin was investigated by a reversed-phase high performance liquid chromatographic method with ultraviolet spectrometrical detection at 210 nm. A column Lichrosphere RP-18, 5 κm particle size, was used as stationary phase, and mixture of methanol, acetonitrile and aqueous phosphate buffer as mobile phase. Improved retention times were achieved, and all the substanced were eluted in 12 minutes. Baseline separation for the three of them, and similar sensitivity of all the five penicillins are the characteristics of

44

chromatogram. The regression data for the quantitative determination of each penicillin are also given in this work. | Rapid separation and quantitative determination of mixtures of amoxicillin, ampicillin, benzylpenicillin, cloxacillin and dicloxacillin was investigated by a reversed-phase high performance liquid chromatographic method with ultraviolet spectrometrical detection at 210 nm. A column Lichrosphere RP-18, 5 κm particle size, was used as stationary phase, and mixture of methanol, acetonitrile and aqueous phosphate buffer as mobile phase. Improved retention times were achieved, and all the substances were eluted in 12 minutes. Baseline separation for the three of them, and similar sensitivity of all the five penicillins are the characteristics of the chromatogram. The regression data for the quantitative determination of each penicillin are also given in this work. Γ10 Separation and determination of carbadox, nitrofurazone, nitrofurantoin,

furazolidone and furaltadone in their mixtures by thin layer and high performance liquid chromatography. I. Caniou, G. Zachariadis, G. Caligas, H. Tsoukali and J. Stratis. Journal of Liquid Chromatography, 1994, 17, 1385-1398.

The separation and quantitative determination of five drugs, namely carbadox, nitrofurazone, nitrofurantoin, furazolidone and furaltadone, in their various mixtures of 3, 4 or 5 components was investigated. Two types of mobile phases were examined for the TLC separation of the drugs, chloroform/acetonitrile/formic acid and chloroform/acetone. Two other mobile phases were also examined for the high performance liquid chromatographic determination of them, acetonitrile/sodium acetate and acetonitrile/sodium dihydrogen phosphate. The resolution of the chromatograms was studied in both cases and also the regression lines of the quantitative determination were described. The absolute detection limits of the determination were in the range of 0.2-1.6 ng for the five compounds. | The separation and quantitative determination of five drugs, namely carbadox, nitrofurazone, nitrofurantoin, furazolidone and furaltadone, in their various mixtures of 3, 4 or 5 components was investigated. Two types of mobile phases were examined for the TLC separation of the drugs, chloroform/acetonitrile/formic acid and chloroform/acetone. Two other mobile phases were also examined for the high performance liquid chromatographic determination of them, acetonitrile/sodium acetate and acetonitrile/sodium dihydrogen phosphate. The resolution of the chromatograms was studied in both cases and also the regression lines of the quantitative determination were described. The absolute detection limits of the determination were in the range of 0.2-1.6 ng for the five compounds. Γ11 Thin layer and high performance liquid chromatographic determination of

histamine in fish tissues. G. Kalligas, I. Kaniou, G. Zachariadis, H. Tsoukali, P. Epivatianos Journal of Liquid Chromatography, 1994, 17, 2457-2468.

TLC and HPLC techniques have been examined for histamine determination in fish. In case of HPLC determination a comparison of the performance characteristics of UV-Vis and Fluorescence detection is also described. Regression analysis was performed between the two sets of data, in order to examine the relative efficiency of the two techniques for the determination of histamine in fish sample extracts. Either of the two techniques proved sufficient for the determination of histamine in common concentration ranges in fish extracts although fluorescence detection had better sensitivity. The recovery of histamine from fish samples was decreased in comparison to the standard solutions but it was still sufficient for routine analysis of the fish samples. | TLC and HPLC techniques have been examined for histamine determination in fish. In case of HPLC determination a comparison of the performance characteristics of UV-Vis and Fluorescence detection is also described. Regression analysis was performed between the two sets of data, in order to examine the relative efficiency of the two techniques for the determination of histamine in fish sample extracts. Either of the two techniques proved sufficient for the determination of histamine in common concentration ranges in fish extracts although fluorescence detection had better sensitivity. The recovery of histamine from fish samples was decreased in comparison to the standard solutions but it was still sufficient for routine analysis of the fish samples. Γ12 Heavy metal pollution in the river Evros, (Southern Europe).

K. Ouzounis, G. Papageorgiou, J. Stratis and G. Zachariadis.

45

Environmental Education and Information, 1994, 13, 31-44. The concentration of some representative heavy metals in a river may indicate its pollution levels and the possible effects on aquatic organisms and the whole ecosystem. The results of such research may be very interesting in the case of a river of special importance such as the Evros, Greece. In this study, the concentrations of iron, manganese, copper, nickel, lead, mercury, zinc, chromium and cadmium were observed at six stations along the river. Analyses were made of both precipitates and fishes in the river. Γ13 Evaluation of chemical parameters in Aliakmon river, Northern Greece.

Part I: Quality characteristics and nutrients. Th. Kouimtzis, C. Samara, D. Voutsa and G. Zachariadis. Journal of Environmental Science and Health, 1994, A29, 2115-2126.

A two-year survey on the water quality characteristics of Aliakmon river in northern Greece is described. Physicochemical parameters (temperature, acidity, alkalinity, conductivity, hardness), as well as nutrients and major ionic components (NO3 -, NH4 +, PO4?, SO4(=), Cl-) were determined and their concentration levels were related to the flow characteristics of the river and the influence from urban, agricultural and industrial activities. The quality of the water is discussed taking into account the fact that this river will be used in the near future for the water supply of the city of Thessaloniki. Γ14 Evaluation of chemical parameters in Aliakmon river, Northern Greece.

Part II: Dissolved and particulate heavy metals. D. Voutsa and G. Zachariadis, C. Samara, Th. Kouimtzis. Journal of Environmental Science and Health, 1995, A30, 1-13.

A two-year survey on the water quality characteristics of Aliakmon river in northern Greece is described. Heavy metals (Pb, Cd, Cr, Cu, Mn and Fe) were determined in the dissolved fraction, as well as in two particulate fractions, labile and residual, separated after a two-step sequential extraction procedure. The partitioning of metals between different fractions was examined at various sampling sites along the river. Variations observed were related to the geology of the transected area and the influence from anthropogenic sources (mainly municipal effluents). Statistical analysis of variance of metal partition coefficient showed significant differences between sampling periods, while insignificant ones between sampling sites. Factor analysis of analytical data yielded four factors that were interpreted as natural, anthropogenic, geological and run-off contributions Γ15 Determination of mercury by electrothermal atomic absorption spectrometry using

different chemical modifiers or a slurry technique. I.Karadjova, P. Mandjukov, S. Tsakovski, V. Simeonov, J. A. Stratis, G. A. Zachariadis. Journal of Analytical Atomic Spectroscopy, 1995, 10, 1065-1068.

The applicability of different chemical modifiers for thermal stabilization and ETAAS determination of mercury is studied. The modifier effect is strongly influenced by the type of acid and acid content in the sample solution. A method is described for mercury determination in tuna fish and sediment after high pressure digestion with nitric acid, using thioacetamide (TAC) as chemical modifier. The method permits determination of 0.5 κg g-1 mercury. A simpler and faster procedure using a slurry technique for the determination of mercury in reference materials (tuna fish, spinach, cabbage and sediments) was also evaluated. Optimal instrumental parameters for mercury determination in slurries are presented. On the basis of the results obtained a new procedure which allows determination of 0.1 κg g-1 mercury was developed. Γ16 A study of air pollution with heavy metals in Thessaloniki city (Greece) using trees

as biological indicators. T. Sawidis, A. Marnasidis, G. Zachariadis and I. Stratis. Archives of Environmental Contamination and Toxicology, 1995, 28, 118-124.

The air pollution of the city of Thessaloniki was studied, using park trees as biomonitors. The species analyzed were Ligustrum japonicum, Nerium oleander, Olea europea, Pinus brutia, Platanus orientalis, Populus alba, Populus nigra, and Robinia pseudoacacia. Acid digestion of leaf

46

tissues and subsequent use of atomic absorption spectrometry was the analytical methodology used for the determination of heavy metals (Cu, Zn, and Pb). The concentration of heavy metals in the tree leaves depended on the metal species as well as on the position of the tree in the city. The mean concentrations of Cu ranged between 5 and 10 mg kg-1 dry weight, of Zn between 19 and 85 mg kg-1 DW and of Pb between <1.5 and 4.5 mg kg-1 DW. In some cases, the effect of road junctions proved more significant than the traffic load for the contamination of leaves. Some differentiation was also observed between tree species. Old leaves collected during the fall were more contaminated than young leaves collected during the spring. The roughness of the outer surface leaves contributed to the trapping and retention of air particles. The results were compared with corresponding results from lichens and mosses which were also collected for a similar study. Γ17 Atomic absorption spectroscopic determination of molybdenum in aqueous tetra-

thiomolybdate solutions. Α. Voulgaropoulos, A. Ayiannidis, J. Stratis, G. Zachariadis, and S. Giroussi. Fresenius Journal of Analytical Chemistry, 1995, 351, 139-140.

The flame atomic absorption spectroscopic determination of molybdenum, in aqueous solutions of tetrathiomolybdate (MoS4 2-, TTM) and paramolybdate species (Mo7O24 2-, PM) has been investigated. A serious signal decrease occurred, if molybdenum has been present as tetrathiomolybdate species, and the sensitivity loss has been 85%. To improve the sensitivity, the solutions of TTM have been digested by wet-acid treatment, and made 0.14 mol·l-1 ammonia. Finally, Mo has been determined by AAS using a nitrous oxide-acetylene flame. The range of the calibration was 0-100 mg·l-1 and the relative standard deviation of the slope was less than 1%. Γ18 Classification of Macedonian Early Bronze Age ceramics using their various

chemical and technological features. J.A. Stratis, G.A. Zachariadis, V.D. Simeonov. Fresenius Journal of Analytical Chemistry, 1995, 351, 697-699.

Twenty-eight early bronze age ceramic sherds from Macedonia, Greece, have been analysed for 12 major and minor chemical components and 5 physicochemical characteristics; the analytical data were treated by hierarchical clustering procedures to obtain different classifications of the sherds. The application of cluster analysis showed that when using only chemical factors of the specimens without simultaneous use of other technological features of the specimens may lead to false conclusions for the archaeologist. This was highlighted with a clay sample 16 among others. Γ19 Determination of chloramphenicol residues in meat samples by high performance

liquid chromatography. I. Caniou, Δ. Νikolaides, H. Tsoukali, J. A. Stratis, G. A. Zachariadis. Journal of Liquid Chromatography, 1995, 18, 3519-3527.

The results of a comparative study of chloramphenicol (CAP) determination of meat, by high performance liquid chromatography are presented. A spectrophotometrical detector at 278 nm was used for measurement of CAP absorbance and subsequent quantitative determination. Two different mobile phases, were tested. The first phase consisted of acetonitrile - water (30:70 v/v) and the second of acetonitrile - di-ammonium hydrogen phosphate 0.005M (25:75 v/v). The two procedures were compared by means of the retention time and the sensitivity achieved. Recoveries of CAP from meat samples ranged between 63-79%. The detection limits for the two procedures were 14.1 κg·kg-1 and 18.0 κg·kg-1 respectively. Γ20 Selective spectrophotometric determination of cobalt(II) using 2,2'-dipyridyl- 2-

pyridyl- hydrazone and a flow injection manifold. D.G. Themelis, G.A. Zachariadis, J.A. Stratis. Analyst, 1995, 120, 1593-1598.

Micro-amounts of cobalt (II) were determined spectrophotometrically at 500 nm using a flow injection manifold and 2,2′-dipyridyl-2-pyridylhydrazone (DPPH) as a colour-forming reagent. The various chemical and physical variables were optimized and a study of interfering ions was also carried out. A linear calibration graph was obtained for 0-12.0 mg l-1 cobalt(II), at a sampling rate of 90 injections per hour. The accuracy and reproducibility were very good (sr = 0.4%), the limit of

47

detection was 0.017 mg l -1 and the limit of quantitation was 0.031 mg l-1. The method was found to be adequately selective owing to the fact that the strongly acidic medium used prevented the formation of complexes of the reagent with other ions. The method has been successfully applied to the determination of cobalt in cyanocobalamin (vitamin B12). Γ21 Heavy metal bioaccumulation in lichens from Macedonia in Northern Greece.

T. Sawidis, M.K. Chettri, G.A. Zachariadis, J.A. Stratis, M. R. D. Seaward. Toxicological and Environmental Chemistry, 1995, 50, 157-166.

An extended research on lichens as biological monitoring indicators is described. The concentration of Pb, Cu, Zn and Cd in lichens from polluted and unpolluted areas of Greece were determined by atomic absorption spectrometry after acid digestion. The levels of the heavy metals in the tissues of different species are discussed. Although bioaccumulation of heavy metals depends on the degree of pollution of the given biotope, large variations were observed between different species collected from the same biotope. In areas with porphyry copper deposits, pendulous lichens hanging from trees and, to a lesser extent epiphytes, were less contaminated than epigeic or epilithic ones. Epilithic lichens accumulated larger concentrations of these metals than did epigeic lichens. Finally, the capacity of lichens to accumulate heavy metals, is compared to that of mosses and tree leaves. Γ22 Heavy metals in aquatic plants and sediments from water systems in Macedonia,

Greece. T. Sawidis, M.K. Chettri, G.A. Zachariadis, J.A. Stratis Ecotoxicology and Environmental Safety, 1995, 32, 73-80.

Heavy metal concentrations were surveyed in several species of aquatic macrophytes and in sediments of a lake and four rivers from Macedonia, northern Greece. Acid digestion of sediments and plant material and the subsequent use of atomic absorption spectrometry were the analytical methodologies followed for the analysis. Values were variable within each species while differences in metal contamination among the studied water ecosystem were also found. The lake was less contaminated than the rivers, the contamination of which is dependent on the metal species. Sediment had greater Cu, Pb, and Ni contamination than the aquatic plants, whereas the reverse was the case for Zn, Cd, and Mn. The mean heavy metal contents of the sediments and aquatic plants were in the descending order of Mn > Zn > Ni > Cu > Pb > Cd, whereas in the accumulation of metals by plant tissues, the corresponding order was root > rhizomes > leaf > flower > stem > seed. Γ23 Critical comparison of wet and dry digestion procedures for trace metal analysis of

meat and fish tissues. G.A. Zachariadis, J.A. Stratis, I. Kaniou and G. Kalligas Mikrochimica Acta, 1995, 119, 191-198.

The efficiency of four methods of digestion was evaluated for trace metal analysis of pork meat and carp fish tissues. Two methods of dry and two methods of wet ashing were compared in terms of calculated variances. Mixtures of HCl+HNO3 were applied for wet ashing of the samples at 100 °C, while dry ashing with or without H2SO4 at 450 °C were the alternative methods. The digests were subsequently analysed for Pb, Cd, Cu and Zn by graphite furnace atomic absorption spectrometry. Analysis of variance and Student's t-test were performed separately for meat and fish analytical results. Wet digestion with a (1+1) mixture of HCl+HNO3 has given better recovery and repeatability for almost all metals than a (9+1) mixture of HCl+HNO3. Also between the dry ashing methods, the use of H2SO4 has given better results than ashing of tissues without H2SO4. Γ24 Chemometrical classification of biomonitoring analytical data for heavy metals.

Part I. Trees (leaves) as bioindicators. J. Α. Stratis, G. Α. Zachariadis, V. Simeonov, P. Mandjukov, T. Sawidis. Toxicological and Environmental Chemistry, 1996, 54, 29-37.

The application of tree species (leaves) as biomonitoring devices is studies by the use of cluster analysis. It is shown that the chemical analysis of leaf samples should be accompanied by a careful chemometrical data treatment in order to obtain a reliable information on the behaviour of various tree species to heavy metal exposure (8 trees for three heavy metals in twelve different

48

places) in fall and spring season. Additionally, the distribution of the sampling sites is studied by the same approach and a commentary on the urban aspects of sampling is given. Γ25 Chemometrical approaches to evaluate analytical data from aquatic macrophytes

and marine algae. J.A. Stratis, V. Simeonov, G.A. Zachariadis, T. Sawidis, P. Madjukov, S. Tsakovski

Fresenius Journal of Analytical Chemistry, 1996, 355, 65-70. The possibility of using aquatic macrophytes and algae as bioindicators of water pollution is investigated. The use of cluster analysis enables the treatment of the analytical data in a global way, revealing hidden clusters of similar behaviour between various biological species or sampling sites. A great number of species was collected from four rivers and fourteen coastal sites in Greece and were analysed for zinc, copper, cadmium, lead and manganese. The analytical data obtained were treated by hierarchical clustering, for marine algae and aquatic macrophytes separately. Γ26 Evaluation of the toxic content of sludges produced during the biological treatment

of municipal and industrial waste waters. D. Voutsa, G. Zachariadis, C. Samara, Th. Kouimtzis. Journal of Environmental Science and Health, 1996,A 31, 657-671. Sewage sludge was periodically sampled from one municipal and one industrial wastewater treatment plant in Thessaloniki, N. Greece, to determine toxic elemental content, as well as seasonal variation. Higher concentrations of As, Cd, Cr, Cu, Hg, Pb, Mn, Zn and Fe were found in the municipal sludge. The application of the US EPA Extraction Procedure of Toxicity showed that both sludges can be characterized as not toxic. Metal leachability was highest from the industrial sludge, which has not subjected to digestion. Leachability of most metals from both sludge types increased under sludge codisposal with either an acidic (gypsum solid waste) or an alkaline material (dolomite). However, in all cases, sludge mixtures were not toxic according to the criteria set by the US EPA for landfilling. Γ27 Evaluation of the quality of municipal and industrial wastewater sludges for

agricultural purposes. D. Voutsa, G. Zachariadis, N. Gantidis, C. Samara, Th. Kouimtzis. Fresenius Environmental Bulletin, 1996, 5, 1-6. Sewage sludge was periodically sampled from one municipal and one industrial wastewater treatment plant in Thessaloniki, N. Greece to determine quality parameters for potentially agricultural utilization. Both sludges were alkaline in nature and had similar nutrient content At an application rate of 10 tn ha-1 y-1, the P additions from sludge will be in excess of crop requirement, wherease supplemental N and K fertilization will be needed to optimize crop yields. Both sludges had low heavy metal content thus meeting the requirements set by the European directive and the Greek law for agricultural use. An application rate of 10 tn ha-1 y-1 of industrial sludge is in agreement with the existing regulations, whereas lower application rates for the municipal sludge should be considered. Γ28 Uptake of heavy metals by living and dead Cladonia Thalli.

M.K. Chettri, T. Sawidis, G.A. Zachariadis, J.A. Stratis Εnvironmental and Experimental Botany, 1997,37,39-52.

Uptake of Pb, Cu and Zn by thalli of Cladonia convoluta (Lam,) and Cladonia rangiformis (Hoffm.) from different concentrations of solutions of Pb(NO3)2, CuCl2 and ZnCl2 was studied. Zinc accumulation was higher in living than in dead thalli, but the opposite occurred for Ph and Cu. Further study with a solution containing all three metals revealed that at higher concentrations, uptake of Zn was very low compared with that for the single-metal solution. Long term exposure of Cladonia living thalli in double deionised water and also in a 10-6 M ZhCl2 solution resulted in leaching of Zn, Leaching of Zn from the thalli was also observed after uptake of Pb or Cu from a single-metal solution. No effects were observed on naturally accumulated PB with uptake of Cu or Zn and on naturally accumulated Cu with uptake of Pb or Zn from a single-metal solution. It can be concluded that Pb, Cu and Zn ions were more strongly hound in C. convoluta than in C. rangiformis.

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Γ29 Soxhlet extraction and acid digestion methods for silver determination in soils by

flame and electrothermal atomic absorption spectrometry. I.N. Papadoyannis, M.K. Sofoniou, V. F. Samanidou, G.A. Zachariadis, A.C Zotou, H.A. Constantinidou and S.E. Tsiouris. Instrumentation Science and Technology, 1997, 25, 283-296.

This paper describes the development of a soxhlet extraction method for the extraction of silver as silver iodide from soil, and its subsequent determination by atomic absorption spectrometry. Silver iodide is extracted as a double salt with potassium iodide in acetone and, after condensation, silver is measured in the extract by flame atomic absorption spectrometry. The efficiency of this extraction was compared to a wet digestion method. Concentrated nitric acid was used for soil digestion and silver was determined in the acid digests by electrothermal atomic absorption spectrometry. In a series of soil samples, silver recovery varied between 90-110%. The detection limits were mass-dependent and, at the experimental conditions described in this work, these were found to be 2 κg/kg in extracted and 3 κg/kg in acid digested soil samples. Γ30 Flame AAS and UV-VIS determination of cobalt , nickel and palladium using the

synergetic effect of 2-benzoylpyridine-2-pyridylhydrazone and thiocyanate ions. G.A. Zachariadis, D.G. Themelis, D.J. Kosseoglou and J.A. Stratis Talanta, 1998, 47, 161-167.

A quantitative synergetic extraction procedure for cobalt, nickel and palladium from thiocyanate aqueous solutions into methyl isobutyl ketone (MIBK), containing 2-benzoylpyridine-2-pyridylhydrazone (BPPH), was studied by flame atomic absorption spectrometry (FAAS) and molecular absorption spectrometry (UV-VIS). Using FAAS, linear calibration graphs were obtained from 0.0-0.5 mg l-1 Co(II), 0.0-1.5 mg l-1 Ni(II) and 0.0-2.0 mg l-1 Pd(II). The reproducibilities were s(r, Co(II)) = 2.0%, s(r, Ni(II)) = 1.0% and s(r, Pd(II)) = 1.3% and the limits of detection were c(L, Co(II)) = 0.004 mg l-1 , c(L, Ni(II)) = 0.009 mg l-1 and c(L, Pd(II)) = 0.012 mg l-1. Using UV-VIS method the linear calibration graphs were 0.0-0.5 mg l-1 for Co(II), 0.0-1.0 mg l-1 for Ni(II) and 0.0-2.0 mg l-1 for Pd(II). The reproducibilities were s(r, Co(II)) = 1.3%, s(r, Ni(II)) = 1.7% and s(r, Pd(II)) = 1.0% and the limits of detection were c(L, Co(II)) = 0.001 mg l-1, c(L, Ni(II)) = 0.004 mg l-1 and c(L, Pd(II)) = 0.002 mg l-1. The extraction method is almost free from interferences and has been successfully applied to the determination of cobalt, nickel and palladium in dental alloys. Γ31 Electrothermal atomic absorption spectrometric determination of aluminium,

cadmium, chromium, copper, iron, manganese, nickel, and lead in olive oils. I. Karadjova, G. Zachariadis, G. Boskou, J. Stratis Journal of Analytical Atomic Spectrometry, 1998, 13, 201-204.

A simple and fast procedure for the direct ETAAS determination of Al, Cd, Cr, Cu, Fe, Mn, Ni and Pb in olive oil is proposed. N,N-Hexamethylenedithiocarbamic acid, hexamethyleneammonium salt (HMDC-HMA), was used as a universal modifier with two functions: isoformation of the chemical species of the investigated elements, present in olive oil and their thermal stabilisation during the pre-treatment step. Various organic solvents were studied and 1,4-dioxane was found to be the most suitable solvent for oil dilution prior to ETAAS analysis. 1,4-Dioxane improves the decomposition of triglycerides during the pre-treatment step and permits aqueous standard solutions to be used for calibration. Uncoated graphite tubes with platforms are proposed as atomisers for the ETAAS determination of Cd, Cu, Fe, Mn and Fe, and pyrolytic graphite coated graphite tubes with grooves are the most suitable atomisers for Al, Cr and Ni. Instrumental parameters optimised according to pre-treatment and atomisation curves, obtained in the presence of the olive oil matrix and with HMDC-HMA as modifier, are presented. The proposed method permits the determination of 0.1 κg g-1 Fe, 0.05 κg g-1 Ni, 0.02 κg g-1 Al, Cu, Cr and Pb, and 0.01 κg g-1 Cd and Mn with relative standard deviations of about 8-10% for all analytes in this concentration range. Γ32 Chemometrical classification of biomonitoring analytical data for heavy metals.

Part II. Mosses as bioindicators. S. Tsakovski, V. Simeonov, Th. Sawidis, G. Zachariadis, J. Stratis Toxicological and Environmental Chemistry, 1999, 69, 287-294.

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The present paper deals with chemometric interpretation of the bioindicating ability of mosses for heavy metal pollution in urban and rural areas of Greece. Fourteen different moss species collected at twelve sampling sites are included in the study. Four heavy metals concentrations (lead, zinc, copper and cadmium) are determined in each of the totally 61 various samples (with respect to plant speciation and site characteristics). Cluster and principal components analysis are applied as classification and display approaches to reveal, on one hand, specific information about mosses specificity towards pollution species and, on the other hand, response of the sampling location towards bioindication by the use of mosses. It is shown that no specific differentiation with respect to the biotope or mosses' family is proved probably, due to lack of sampling design strategy rather than to equal bioindicator response or similar ecological situation in the regions of sampling. Specific detection is proved mainly in region with well-expressed pollution. Γ33 Chemometrical classification of biomonitoring analytical data for heavy metals.

Part III. Lichens as bioindicators. J. A. Stratis, S. Tsakovski, V. Simeonov, G. Zachariadis, Th. Sawidis, Toxicological and Environmental Chemistry, 1999, 69, 295-304.

The present study deals with the application of lichens as bioindicators in a large region of Northern Greece including rural and urban sites. Various types of lichens are collected throughout the sampling procedure and the concentrations of four heavy metals (zinc, lead, copper and cadmium) are determined in the bioindicator species by the use of atomic absorption spectrometry. The analytical data is then treated by hard and crisp clustering approaches to evaluate some similarities in the sampling sites and, if possible to report on some specific bioindicating. It is shown that the sampling areas are grouped with respect to their ecological situation and the similarity is proved within the clusters of 'rural', 'urban', 'anthropogenically influenced' sites. No lichen selectivity or specificity with respect to certain heavy metal is proved which may be due to the undesigned sampling procedure. Γ34 Effects of cadmium exposure on bioaccumulation and larval growth in the frog

Rana ridibunda. N.S. Loumbourdis, P. Kyriakopoulou-Sklavounou, G. Zachariadis. Environmental Pollution, 1999, 104, 429-433.

The bioaccumulation of cadmium and the effect on growth of larvae of the frog Rana ridibunda after exposure to Cd for 15 and 30 days was investigated. The 96-h LC50 value was 71.8 ppm. The water content of all the tadpoles exposed to all cadmium concentrations was higher than that of controls. There was a tendency for the animals exposed to Cd to retard their growth. Mortality rates at 15 days exposure increased with increasing cadmium concentration and remained constant in all the cadmium concentrations at 30 days exposure. Cadmium accumulation was higher in the 15 days exposed tadpoles, compared to that of 30 days exposure. Growth retardation may be a factor contributing to the declining of amphibian populations, since they, because of their smaller size, spend more time in more vulnerable stages, becoming thus more susceptible to predators. Γ35 Use of macroalgae as biological indicators of heavy metal pollution in Thermaikos

gulf, Greece. K. Fytianos, E. Evgenidou, G. Zachariadis. Bulletin of Environmental Contamination Toxicology, 1999, 62, 630-637.

The present study describes an approach to heavy metal estimation in a particular area of the Northern Aegean Sea, the Thermaikos Gulf. Macroalgae were sampled in four stations representative of three areas of Thermaikos Gulf. The city of Thessaloniki with more than 1.200.000 inhabitants and its surrounding industrial area use Thermaikos Gulf as the final reservoir for their wastewaters. Thermaikos Gulf, which has an overall perimeter of about 70 km, consist of two basins, the inner and the central bay, having a small mean depth of about 20 m. The two basins communicate together and to the opean sea by narrow and shallow mouths, which do not permit the streams to carry off the wastes into the open sea for keeping the pollution on low levels. In Greece, certain coastal areas and especially several closed gulfs such as the Thermaikos Gulf in North Aegean Sea have been in the recent years very rapidly affected by industrialization, which is taking place without the adequate provisions for protective measures to

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maintain the quality of the marine environment. About 120.000 m3/day of untreated and partially treated (40.000 m3/day) sewage water from the city of Thessaloniki and an amount of about 30.000 m3/day of treated/untreated industrial effluents are discharged on the north western coast of the Gulf. Γ36 Spectrophotometric determination of phenols and cyanides after distillation from

natural waters. M.K.Sofoniou, G.A.Zachariadis, A.N.Anthemidis, Th.A. Kouimtzis.

International Journal of Environmental Analytical Chemistry, 2000, 78, 353-365. Total phenols were determined by molecular spectrophotometry, after distillation, complexation with 4-aminoantipyrine and extraction into chloroform. Cyanides were also determined spectrophotometrically after distillation from the acidified samples, and complexation in moderate acidic solution with barbituric acid. The dynamic ranges were 0 - 100 κg L -1 for total phenols and 0 - 30 κg L -1 for cyanides. The above methods were applied in the analysis of river, lake and stream waters collected from Northem Greece. The seasonal and spatial variation of concentrations was evaluated by two-way ANOVA. Background levels (4 - 12 κg L -1 for total phenols and 0.3 - 3 κg L -1 for cyanides), were found in almost all surface waters, with some exceptions. Γ37 Profile of heavy metals distribution in water and sediment samples from river Evros

(Southeast Europe). G. Vasilikiotis, G. Zachariadis, J. Stratis, F. Vosniakos Journal of Environmental Protection and Ecology, 2000, 1, 58-62. This is a study of the anthropogenic contamination of water and sediments of River Evros in Northern Greece with several heavy and toxic metals like Pb, Mn, Cu θαη Zn. All the analytes were determined by Atomic Absorption Spectrometric techniques . The results showed that zinc and manganese appear in the water in higher concentrations than lead or copper at least by an order of magnitude. In sediments the presence of manganese has a geological origin. The results are compared with a previous study in the same aquatic system.

Γ38 SEM-EDXRF study of two chemical modifiers based on platinum and tungsten, and

their effect on ETAAS atomization of lead. G.A. Zachariadis, S.A. Sklavounos, P.B. Mandjukov, J.A. Stratis. Mikrochimika Acta, 2001, 136, 115-122.

The behavior of two chemical modifiers based on platinum and tungsten, is studied in the two parts of this work. At first, the chemical and morphological alterations of these during pyrolysis and atomization stages in electrothermal atomic absorption spectrometry are studied. The compounds were H2PtCI6 and Na2WO4. A cylindrical graphite tube was used, and fixed shaped rectangular graphite plates were put inside as platforms to prepare the specimens. The crystalline formations on the platforms were studied by scanning electron microscopy and the energy dispersive X-ray spectra allowed the estimation of the chemical composition changes of the modifiers and the graphite surface. According to the spectra and the images obtained, both modifiers where found to change their crystalline form at temperatures between 500-1250°C. Severe corrosion of the graphite surface was observed in case of H2PtCl6 at temperature higher than 750°C, while minor damages were observed in case of Na2WO4. In the second part of this work, the efficiency of these modifiers was tested for maximum loss-free pyrolysis temperature and atomization of lead by electrothermal atomic absorption spectrometry. The optimum results, concerning these parameters, obtained for medium concentration of the modifiers. The effect of each modifier on the atomization signal is discussed, and the optimum concentration of each one is estimated. Γ39 Determination of lead, cadmium and mercury in surface marine sediments and

mussels. G.A.Zachariadis, A.N.Anthemidis, I.Caniou, J.A.Stratis. International Journal of Environmental Analytical Chemistry, 2001 80, 153-166.

Lead, cadmium and mercury were determined in sediments and mussels, and the ability of these indicators to record metal variations in coastal marine environment is described in this work. The results of an extended investigation of the status of three gulfs at Northern Greece are given,

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regarding the content of these metals in surface sediments and Mytilus galloprovincialis. The samples were collected during a four-year period. The total concentration of the above heavy metals was determined after digestion of the samples by suitable mixtures of acids, including nitric, perchloric and hydrofluoric acid. The digestion was carried out in a steel pressurised bomb with closed teflon vessels. Lead and cadmium were determined by means of electrothermal atomic absorption spectrometry (ETAAS), and mercury by cold vapour atomic absorption spectrometry (CVAAS). The results were statistically evaluated by analysis of variance, and emphasis was given to annual, seasonal and spatial sources of variation. The annual changes during the last four years and the spatial distribution of heavy metals load is also discussed. Γ40 A study of metal distribution from lignite fuels using trees as biological monitors. T. Sawidis, M.K. Chettri, A. Papaioannou, G. Zachariadis, J. Stratis.

Ecotoxicology and Environmental Safety, 2001, 48, 27-35. Concentrations of five metals (Cd, Cu, Mn, Fe, Zn) were determined in tree leaves collected from an area with large coal-fired plants in Ptolemais, Macedonia, Greece. The analyses were carried out with flame atomic absorption spectroscopy. Mean heavy metal content in the tree leaves is, in descending order, Fe > Mn > Zn > Cu > Cd. In Populus nigra and Salix babylonica the dense piled leaves and the widespread root system are the reasons for high heavy metal uptake. Conifer trees Pinus nigra and Juniperus arizona, which have a rough leaf surface, also had elevated concentrations. Leaves with a smooth surface were less contaminated. The presence of one metal within leaf cells may reduce the uptake or toxicity of other metals. Fruits accumulated less heavy metal than the corresponding leaves. The most damaged areas are those in the direct vicinity of the coal power plants, and the rate of damage caused in the environment is affected by the main wind direction. All sampling sites in Ptolemais basin are generally more or less polluted when compared with the control site.

Γ41 On-line solid phase extraction system using PTFE packed column for the flame

atomic absorption spectrometric determination of copper in water samples. A.N. Anthemidis, G.A. Zachariadis, J.A. Stratis.

Talanta 2001, 54, 935-942. A new flow injection on-line adsorption preconcentration system adapted to flame atomic absorption spectrometry (FAAS) for copper determination at the κg 1-1 level was developed. Polytetrafluoroethylene (PTFE) turnings packed in a mini-column were used as sorbent material. The copper ammonium pyrrolidine dithiocarbamate (APDC) complex was sorbed on the PTFE turnings, from which it could be eluted on-line instantly by isobutyl methyl ketone (IBMK) into the flame at a flow rate of 2.3 ml min-1. The system was optimized and offered good performance characteristics with practically unlimited life time, greater flow rates and improved flexibility, as compared with other sorbent materials and the knotted reactor preconcentration systems. With 1 min preconcentration time, and a sample frequency of 40 h-1, the enhancement factor was 340, which could be further improved by increasing the preconcentration time. The detection limit was cL = 0.05 κg 1-1, and the precision was 1.5%, at the 2.0 κg 1-1 Cu level. The method has been applied successfully to the analysis of potable, river and seawater, and its accuracy was tested by the analysis of certified reference materials and by recovery measurements on spiked samples. No significant interferences exist from other substances usually occurring in natural water. Γ42 Trace metal concentrations in marine macroalgae from different biotopes in Aegean

Sea. T. Sawidis, M.T. Brown, G. Zachariadis, J. Stratis Εnvironment International, 2001, 27, 43-47.

The commonest species of red, brown, and green macroalgae were sampled from a range of biotopes in the Aegean Sea and analysed for five different trace metals. Significant differences in metal concentrations were found among different seaweed species from the same biotope. The concentrations of metals in the various seaweed species may reflect their morphology, with those having a larger surface area having a greater internal content. Different species of seaweed have different affinities for different heavy metals. This may reflect competition between metals for binding or uptake sites in the seaweed: Comparing metal concentrations in algae among the studied sampling stations clearly indicates that the degree of accumulation depends not only on

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human activities but also on the geology of the specific area. While seaweed can be used successfully to assess the levels of heavy metals in the marine environment, not all elevated concentrations of heavy metals necessarily reflect increased levels of pollution. Indeed, the high concentrations of certain metals, e.g., Ni, found in our seaweed samples reflected the metaliferrous nature of the rock. It is therefore important to take account of a region's geology before attempting to interpret the data. Γ43 Accumulation of heavy metals in vegetables grown in industrial area in relation to

soil. K. Fytianos, G. Katsianis, P. Triantafyllou, G. Zachariadis. Bulletin of Environmental Contamination Toxicology, 2001, 67, 423-430.

This paper describes a study of the accumulation of heavy metals in various vegetables in relation to the levels of the metals in corresponding soil samples. Flame atomic absorption spectrometry was employed and a method to determine the total concentration of Pb, Cd, Ni, Cu, Mn, Zn, Fe after complete wet acid decomposition of the samples using a mixture of HNO3-HClO4. Two regions were sampled, one with only agricultural activities and another from an industrial area. Higher concentrations of several heavy metals were observed in the samples from the more polluted areas, although in comparison to literature data from other areas, the estimated levels were comparable to those from unaffected regions. Γ44 Determination of mineral content of active dry yeast used in pharmaceutical

formulations G.A. Zachariadis, E.S. Raidou, D.G. Themelis, J.A. Stratis.

Journal of Biomedical and Pharmaceutical Analysis, 2002,28, 463-473. The efficiency of seven common methods of digestion of active dry yeast (ADY), which is used in anticariogenic dental formulations, was evaluated for the analytical determination of Fe, Zn, Ca, Mg, Na and K. Four wet-acid digestion and three dry ashing methods are compared in consideration of their estimated reproducibility and metal concentrations obtained. HNO 3, HNO 3+HCl, HNO 3+H 2SO 4 and HNO 3+HClO 4 were applied for wet digestion of the samples in medium temperatures, while dry ashing at higher temperature with Mg(NO 3) 2 or SrCl 2 as ashing aid agents, were the alternative methods. The final solutions were subsequently analyzed for Fe, Zn, Ca and Mg by flame atomic absorption spectroscopy (FAAS) and for Na and K by atomic emission spectroscopy (AES). Two multivariate statistical methodologies, Analysis of variance (ANOVA) and the Kruskal-Wallis test were applied for the interpretation of the results. Seven additional statistical tests (least-significant difference, Bonferoni, Duncan multiple range, Student-Newman-Keuls, Tuckey significant difference, Tuckey b and Scheffe) were used and proved useful to estimate which of the decomposition methods are outliers. The ideographic approach enabled the comparison of the methods in terms of complexity and difficulty of their steps. Zn and Mg could be reliably determined by any one of the tested methods, while for the other elements, the most powerful method was found and the obtained recoveries were found (>95%). Γ45 Flame atomic absorption spectrometric determination of chromium(VI) by on-line

preconcentration system using a PTFE packed column. A.N. Anthemidis, G.A. Zachariadis, J. Kougoulis and J.A. Stratis Talanta, 2002, 57, 15-22.

A new, sensitive and robust time-based flow injection (FI) method for on-line preconcentration and determination of ultra trace amounts of chromium(VI) by flame atomic absorption spectrometry (FAAS) has been elaborated. The sample is initially mixed on-line with ammonium pyrrolidine dithiocarbamate (APDC) and the Cr(VI)-PDC chelate is absorbed quantitatively on a mini-column packed with polytetrafluoroethylene (PTFE) turnings at a pH range 0.8-1.4. The complex is subsequently eluted with isobutyl methyl ketone (IBMK) and introduced directly into the nebulizer-burner system. The optimized system offered improved performance characteristics, with unlimited lifetime of the proposed column. The enhancement factor was 80, for a 3-min preconcentration time and the sample frequency was 18 h-1. The calibration curve was linear over the concentration range 1-40 κg 1-1 with a detection limit of cL = 0.8 κg 1-1 and a relative standard deviation of sr = 3.2%, at the 20 κg 1-1 level. The proposed method was evaluated by

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analyzing samples of certified and spiked water, and it was applied to the analysis of natural water samples and sediments. Γ46 Assesment of the quality characteristics of Pinios river, Greece.

K. Fytianos, A. Siumka, G. Zachariadis, S. Beltsios. Water, Air and Soil Pollution, 2002, 136, 317-329.

The results of a one-year detailed water quality study of the Pinios river are presented. Physicochemical parameters (pH, conductivity, DO) did not show significant differences neither between sampling sites, nor between sampling periods. Nutrients (nitrogen and phosphorus compounds) showed temporal variations probably caused by seasonal variations in runoff nutrient from weather events. Heavy metals (Pb, Cd, Cr, Cu, Ni) were determined in the dissolved fraction and the variations observed were related to the geology of the examined area and the influence from anthropogenic sources. Statistical analysis of variance of the most important chemical parameters and heavy metals showed significant variation between sampling periods, but insignificant ones between sampling sites. Γ47 Determination of lead by on-line solid phase extraction using a PTFE micro-column

and flame atomic absorption spectrometry. G.A. Zachariadis, A.N. Anthemidis, P.G. Bettas and J.A. Stratis Τalanta, 2002, 57, 919-927.

A rapid and sensitive time-based flow injection (FI) method for on-line preconcentration and determination of lead by flame atomic absorption spectrometry (FAAS), using polytetrafluoroethylene (PTFE) turnings as packing material in a micro-column, has been developed. The sample was mixed on-line with ammonium pyrrolidine dithiocarbamate (APDC) and the non-charged Pb(II)-PDC complex was absorbed quantitatively on the hydrophobic PTFE material, at a pH range 1,4-3.2. The preconcentrated complex was effectively eluted with isobutyl methyl ketone (IBMK) and introduced into the nebulizer-burner system. A nested coil (NC) is proposed for parking the eluate temporarily, in order to enable different elution and nebulization flow rates. With 180 s preconcentration time the sample frequency was 15 h -1, and the enhancement factor was 330 at 13.0 ml min -1 sample flow rate. The detection limit was C L = 0.8 κg 1 -1, the relative standard deviation (R.S.D.) 2.6% at the 30 κg 1 -1 level and the calibration curve was linear over the concentration range 1.6-100 κg 1 -1. The proposed method was evaluated by analyzing certified reference materials of water, sediments and fish tissue. Finally, it was applied successfully to the analysis of various environmental samples. Γ48 Soil silver content of agricultural areas subjected to cloudseeding with silver iodide.

S.E.Tsiouris, F.A.Aravanopoulos, I.N.Papadoyannis, M.K.Sofoniou, N.Polyzopoulos, M. Christodoulou, V.F. Samanidou, G.A. Zachariadis, H.A. Constantinidou

Fresenius Environmental Bulletin, 2002, 11, 697-702. The Hellenic Organization of Agricultural Insurance carried out cloud seeding with silver iodide (AgI) over two agricultural regions in Northern Greece, Hemathia-Pella and Serres, for 12 years in the former and 13 years in the latter, in order to suppress hailstone formation. The possibility of soil pollution due to Ag input was investigated by analyzing 2500 soil samples. A spectrophotometric method capable of detecting Ag in ppb quantities in soil samples was developed, reflecting the low total amount of AgI used in cloud seeding in the two regions (469 and 361 kg, respectively). Average Ag concentrations in soils of Hemathia-Pella (45 ppb) and Serres (37 ppb) were found to be within the range of those in control areas and comparable to those reported in the literature for unpolluted soils. Γ49 On-line preconcentration and determination of copper, lead and chromium(VI) using

unloaded polyurethane foam packed column by flame atomic absorption spectrometry in natural waters and biological samples. A.N. Anthemidis, G.A. Zachariadis, J.A. Stratis Τalanta, 2002, 58, 831-840.

A simple, sensitive and low cost, flow injection time-based method was developed for on-line preconcentration and determination of copper, lead and chromium(VI) at sub κg1-1 levels in natural waters and biological samples. At the optimum pH, the on-line formed metal-ammonium

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pyrrolidine dithiocarbamate (APDC) complexes were sorbed on the unloaded commercial polyurethane foam (PUF), and subsequent eluted quantitatively by isobutylmethylketone and determined by flame atomic absorption spectrometry (FAAS). All chemical, and flow injection variables were optimized for the quantitative preconcentration of each metal and a study of interference level of various ions was also carried out. The system offered improved flexibility, low backpressure and applicability to all the studied metals. At a sample frequency of 36 h-1 and a 60 s preconcentration time, the enhancement factor was 170, 131 and 28, the detection limit was 0.2, 1.8 and 2.0 κg 1-1, and the precision, expressed as relative standard deviation (sr), was 2.8 (at 10 κg 1-1), 3.4 (at 50 κg 1-1) and 3.6% (at 50 κg 1-1) for Cu(II), Pb(II) and Cr(VI), respectively. The accuracy of the developed method was sufficient and evaluated by the analysis of certified reference materials and spiked water samples. Finally, the method was applied to the analysis of environmental samples. Γ50 Cobalt ultra trace on-line preconcentration and determination using a PTFE turnings

packed column and electrothermal atomic absorption spectrometry. Applications in natural waters and biological samples A. N. Anthemidis, G.A. Zachariadis, J.A. Stratis Journal of Analytical Atomic Spectroscopy, 2002, 17, 1330-1334.

A novel flow injection (FI) on-line column preconcentration system, coupled to electrothermal atomic absorption spectrometry (ETAAS) for ultra-trace cobalt determination, has been developed. Polytetrafluoroethylene (PTFE) turnings are used as sorbent material. The sample is mixed on-line with ammonium pyrrolidine dithiocarbamate (APDC) and the non-charged CoII-PDC complex is retained on the hydrophobic PTFE turnings at a pH range of 5.5-7.0. The preconcentrated complex is effectively eluted with a discrete zone of 35 κl isobutyl methyl ketone (IBMK). Airflow is used to introduce the eluent into the transversely heated graphite tube atomizer, through the column. With 60 s preconcentration time and 11.5 ml min-1 sample flow rate the enhancement factor is 87 and the sample frequency is 20 h-1. The detection limit is cL = 4 ng l-1, the relative standard deviation is 4.5% at the 0.1 κg l-1 level and the calibration curve is linear over the concentration range 0.01-0.25 κg l-1. The proposed method was evaluated by analyzing certified reference materials of water and mussel tissue. Finally, it was successfully applied to the analysis of natural waters and biological samples Γ51 Evaluation of different calibration methods in inductively coupled plasma atomic

emission spectrometric analysis of certified glass materials and archaeological glass specimens G.A. Zachariadis, A.N. Anthemidis, J.A. Stratis Journal of Analytical Atomic Spectrometry, 2003, 18, 358-366.

An extended study of the effect of the calibration strategy followed in multi-element Analysis of glass matrices by inductively coupled plasma atomic emission spectrometry (ICP-AES) is reported. The accuracy of the determination of two major (Ca, Na) and four minor constituents (Al, Fe, Mg, Ba) was evaluated by Anal. yzing two certified reference glass materials (SRM 620 and SRM 621) by three different calibration methods, including multi-element and single-element standards. The effect of sample dilution and the presence of high concentrations of some major constituents was also investigated. Two-way Analysis of variance was applied at a given level of confidence (95%) to evaluate the significance of these effects as sources of variation, while the slope of the calibration curves was used as a reliable criterion for the evaluation of the different calibration processes. The recoveries obtained from the two SRMs were also found. Finally, the three calibration methods were applied successfully in the Analysis of archaeological glass fragments and glassy specimens from an excavated ancient oven. Γ52 Gallium trace on-line preconcentration / separation and determination using a

polyurethane foam mini-column and flame atomic absorption spectrometry. Application in aluminum alloys, natural waters and urine. A. N. Anthemidis, G.A. Zachariadis, J.A. Stratis Talanta 2003, 60, 929-936.

A sensitive and selective flow injection time-based method for on-line preconcentration/separation and determination of gallium by flame atomic absorption spectrometry at trace levels was

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developed. The on-line formed gallium chloride complex is sorbed onto a polyether-type polyurethane foam mini-column, followed by on-line quantitative elution with isobutyl methyl ketone and direct introduction into the flame pneumatic nebulizer of the atomic absorption spectrometer. All chemical and flow variables of the system as well as the possible interferences were studied. The manner of strong HCl solutions propulsion was investigated and established using a combination of two displacement bottles. For 90 s preconcentration time, a sample frequency of 28 h-1, an enhancement factor of 40, a detection limit of 6 κg 1-1 and a precision expressed as relative standard deviation (sr) of 3.3% (at 1.00 mg 1-1) were achieved. The calibration curve is linear over the concentration range 0.02-3.00 mg 1-1. The accuracy of the developed method was sufficient and evaluated by the analysis of a silicon-aluminum alloy standard reference material. Finally, it was successfully applied to gallium determination in commercial aluminum alloys, natural waters and urine. Γ53 On-line preconcentration of mercury using an integrated column / gas-liquid

separator (PCGLS) and cold vapor atomic absorption spectrometry. G.A. Zachariadis, A.N. Anthemidis, M. Karpouzi, J.A. Stratis Journal of Analytical Atomic Spectrometry, 2003, 18, 1274-1278.

A novel flow injection (FI) method for the determination of mercury at ng 1-1 levels was developed, using on-line solid phase extraction and cold vapour atomic absorption spectrometry (CVAAS). The pyrrolidine dithiocarbamate mercury complex Hg(PDC)2, is adsorbed and reduced to elemental mercury, and the generated vapour is separated in a newly specifically designed and constructed unified and combined preconcentration column/gas-liquid separator (PCGLS). The lower of the two sections of PCGLS is a preconcentration column packed with polytetrafluoroethylene (PTFE) turnings for the retention of Hg(PDC)2 and subsequent on-column reduction of mercury by SnCl2. Mercury vapour is generated within the column and expelled from the reaction mixture by passing nitrogen through the PCGLS. The upper section of the PCGLS is a headspace for receiving mercury vapour and separating it from humidity residues before entering the absorption cell. The use of strong inorganic acids or volatile organic solvents as eluents is avoided, because the elution step is eliminated. The excellent performance of PTFE as packing material and the compact design of the PCGLS results in higher sensitivity and sampling frequency and to a more functional and simple manifold. An enrichment factor of 32, a detection limit of cL = 6 ng 1 -1, a sampling frequency of 30 h-1 were obtained at sample flow rate of 11.5 ml min-1, for 60 s preconcentration time. The precision, expressed as relative standard deviation, at 0.6 κg l-1 Hg(n), was sr = 2.1%. The proposed method overcomes the common interferences including those from volatile organic solvents and has been successfully applied to the determination of inorganic mercury in natural waters, and total mercury in urine and mussel samples after digestion. Γ54 Development of an on-line solvent extraction system for electrothermal atomic

absorption spectrometry utilizing a new gravitational phase separator. Determination of cadmium in natural waters and urine samples. A. N. Anthemidis, G.A. Zachariadis, J.A. Stratis Journal of Analytical Atomic Spectrometry, 2003, 18, 1400-1403.

A novel flow injection (FI) on-line solvent extraction preconcentration/ separation system coupled to electrothermal atomic absorption spectrometry (ETAAS), which can tolerate high aqueous to organic flow rate ratios, was developed. The organic extract was effectively separated from a large volume of aqueous phase in a newly designed gravitational phase separator. The performance of the system was illustrated for ultra-trace cadmium determination using ammonium diethyldithiophosphate (DDPA) as chelating agent and isobutyl methyl ketone (IBMK) as extractant. The ETAAS determination and solvent extraction are successfully synchronized through a parallel-operated sequence. The obtained enrichment factor was 24.6 with a sampling frequency of 30 h-1. The detection limit was 2.8 ng 1-1 and the precision (RSD at 0.2 κg 1-1 Cd) was 3.2%. The proposed method was evaluated by analyzing certified reference materials and applied to the analysis of natural waters and urine samples. Γ55 Assesment of the surface water quality in Northern Greece.

V. Simeonov, J.A. Stratis, C. Samara, G. Zachariadis, D. Voutsa, A. Anthemidis,

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M. Sofoniou, Th. Kouimtzis Water Research, 2003, 37, 4119-4124.

The application of different multivariate statistical approaches for the interpretation of a large and complex data matrix obtained during a monitoring program of surface waters in Northern Greece is presented in this study. The dataset consists of analytical results from a 3-yr survey conducted in the major river systems (Aliakmon, Axios, Gallikos, Loudias and Strymon) as well as streams, tributaries and ditches. Twenty-seven parameters have been monitored on 25 key sampling sites on monthly basis (total of 22,350 observations). The dataset was treated using cluster analysis (CA), principal component analysis and multiple regression analysis on principal components. CA showed four different groups of similarity between the sampling sites reflecting the different physicochemical characteristics and pollution levels of the studied water systems. Six latent factors were identified as responsible for the data structure explaining 90% of the total variance of the dataset and are conditionally named organic, nutrient, physicochemical, weathering, soil-leaching and toxic-anthropogenic factors. A multivariate receptor model was also applied for source apportionment estimating the contribution of identified sources to the concentration of the physicochemical parameters. This study presents the necessity and usefulness of multivariate statistical assessment of large and complex databases in order to get better information about the quality of surface water, the design of sampling and analytical protocols and the effective pollution control/management of the surface waters. Γ56 Soil silver mobility in areas subjected to cloud seeding with AgI

Tsiouris SE, Aravanopoulos FA, Papadoyannis IN, Sofoniou MK, Samanidou VF, ZachariadisGA, Constantinidou Δ. Fresenius Environmental Bulletin, 2003, 12, 1059-1063.

Agricultural areas in Northern Greece were subjected to cloud seeding with AgI over a period of 12 years in order to suppress hailstone formation and protect crops from damage. Potential soil pollution due to the Ag used and Ag mobility were investigated. Soil samples taken from undisturbed surface soils and from three soil depths were analysed. Average Ag concentrations at soil depths of 0-10, 10-20 and 20-30 cm were 87, 51 and 43 ppb, respectively. Silver mobility through various soil substrates and into plants was investigated in a two-year pot experiment. Silver iodide or AgNO 3 were applied to the pot soil surface and grass species seeded were periodically watered, simulating a natural rainfall pattern. Silver was quantified in water leachates, in grass shoots, and in the pot soil profile at the end of the experiment. Silver was not detected in water leachates, exhibiting a very slow motion through the soil columns. Plants absorbed Ag, but in minute quantities, when supplied in the form of AgI, whereas appreciable amounts of Ag were absorbed from the much more soluble AgNO3 form.

Γ57 On-line liquid-liquid extraction system using a new phase separator for flame

atomic absorption spectrometric determination of ultra-trace cadmium in natural waters A. N. Anthemidis, G. A. Zachariadis, Ch. G. Farastelis, J. A. Stratis

Talanta, 2004, 62, 437-443. A robust flow injection (FI) on-line liquid-liquid extraction (LLE) preconcentration/separation system associated with a newly designed gravitational phase separator, coupled to flame atomic absorption spectrometry (FAAS) was developed. The performance of the system was illustrated for cadmium determination at the κgl-1 level. The non-charged cadmium complex with ammonium pyrrolidine dithiocarbamate (APDC) was extracted on-line into isobutyl methyl ketone (IBMK). The organic phase was effectively separated from a large volume of aqueous phase and is led into a 100κl loop of an injection valve before its introduction into the nebulizer. The system was optimized and offered good performance characteristics with unlimited life time of phase separator, greater flow rate ratios and improved flexibility, as compared with other solvent extraction preconcentration systems. With a sampling frequency of 33h-1, the enhancement factor was 155, the detection limit was 0.02κgl-1, the relative standard deviation was 3.2% at 2.0κgl-1 Cd concentration level and the calibration curve was linear over the concentration range 0.06-6.0κgl-1. The accuracy of the proposed method was evaluated by analyzing a certified reference material of water and by recovery measurements on spiked samples. Finally, it was successfully applied to the analysis of tapwater, river and seawater samples.

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Γ58 Microwave-assisted versus conventional decomposition procedures applied on a

ceramic potsherd standard reference material by inductively coupled plasma atomic emission spectroscopy D.N. Papadopoulou, G.A. Zachariadis, A.N. Anthemidis, N.C. Tsirliganis, J.A. Stratis

Analytica Chimica Acta, 2004, 505, 173-181. Inductively coupled plasma atomic emission spectrometry (ICP-AES) is a powerful, sensitive analytical technique with numerous applications in chemical characterization including that of ancient pottery, mainly due to its multi-element character, and the relatively short time required for the analysis. A critical step in characterization studies of ancient pottery is the selection of a suitable decomposition procedure for the ceramic matrix. The current work presents the results of a comparative study of six decomposition procedures applied on a standard ceramic potsherd reference material, SARM 69. The investigated decomposition procedures included three microwave-assisted decomposition procedures, one wet decomposition (WD) procedure by conventional heating, one combined microwave-assisted and conventional heating WD procedure, and one fusion procedure. Chemical analysis was carried out by ICP-AES. Five major (Si, Al, Fe, Ca, Mg), three minor (Mn, Ba, Ti) and two trace (Cu, Co) elements were determined and compared with their certified values. Quantitation was performed at two different spectral lines for each element and multi-element matrix-matched calibration standards were used. The recovery values for the six decomposition procedures ranged between 75 and 110% with a few notable exceptions. Data were processed statistically in order to evaluate the investigated decomposition procedures in terms of recovery, accuracy and precision, and eventually select the most appropriate one for ancient pottery analysis.

Γ59 Human recombinant mutated forms of the mitochondrial COΥ assembly Sco2

protein differ from wild-type in physical state and copper binding capacity. P.F. Foltopoulou, G.A. Zachariadis, A.S. Politou, A.S. Tsiftsoglou, L.C. Papadopoulou Molecular Genetics and Metabolism, 2004, 81, 225-236.

The human Sco2 protein is a cytochrome c oxidase assembly protein that participates in mitochondrial copper pathway, acting downstream of Cox17 protein. In a previous work, we detected mutations in the human SCO2 gene in three unrelated infants with fatal cardioencephalomyopathy and COX deficiency. In this study, full-length processed recombinant wild-type and two mutated forms of hSco2p (w/t-rhSco2p, E140K-rhSco2p, and S225F-rhSco2p) were produced in bacteria as soluble recombinant peptides for the first time and evaluated for differences in their physical state and ability to bind copper. Our data indicate the following: (a) w/t-rhSco2p and S225F-rhSco2p were found to be in a monomeric form in contrast to E140K-rhSco2p that was in a major non-reducible dimer and a minor monomer form; (b) wild-type and mutated rhSco2p exhibited clear differences in their physical conformational state, as shown by circular dichroism and thermal denaturation analyses; (c) copper binding studies showed that E140K-rhSco2p bound markedly less copper while S225F-rhSco2p more than expected as compared to amount of the copper bound with w/t-rhSco2p. rhCox17p served as positive control experiment. These data indicate that S225F and E140K mutations found in the SCO2 gene derived from patients alter the physical conformational state of encoded hSco2p that may disturb the normal copper transport pathway in mitochondria. These findings are valuable for understanding the molecular basis of fatal cardioencephalomyopathy and COX deficiency and for designing appropriate pharmacological interventions. Γ60 Time-based injection on-line preconcentration cold vapour generation procedure for

ultra-trace mercury determination with inductively coupled plasma atomic emission spectrometry. A.N. Anthemidis, G.A. Zachariadis, C.E. Michos, J.A. Stratis Analytical and Bioanalytical Chemistry, 2004, 379, 764-769.

A time-based sequential dispensing on-line column preconcentration procedure for mercury determination at trace levels by cold vapour generation inductively coupled plasma atomic emission spectrometry (CV-ICP-AES), by means of a unified module of a preconcentration column and a gas-liquid separator (PCGLS) is described. The complex of mercury formed on-line with

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ammonium pyrrolidine dithiocarbamate (APDC) is retained on the surface of the hydrophobic poly(tetrafluoroethylene) (PTFE) turnings, which are packed into the lower compartment of the PCGLS. Subsequently, mercury vapour is generated directly on the PTFE turnings by reductant SnCl2 and separated from the liquid mixture via the PCGLS by argon purge gas. The outlet of the PCGLS is connected directly to the torch adapter of the plasma without the normal spray chamber and nebulizer. With 60-s preconcentration time and 12.0 mL min-1 sample flow rate, the sampling frequency is 30 h-1. The calibration curve is linear over the concentration range 0.02-5.0 κg L-1, the detection limit (c L) is 0.01 κg L-1 and the relative standard deviation (s r) is 3.1% at the 1.0 κg L-1 level. The proposed method was evaluated by analysis of BCR CRM 278 (Mytilus Edulis) reference material and applied to the determination of total mercury in digested urine, blood and hair samples. Γ61 Development of a sequential injection system for trace mercury determination by

cold vapour atomic absorption spectrometry utilizing an integrated gas liquid separator / reactor.

A. N. Anthemidis, G. A. Zachariadis, J.A. Stratis Talanta, 2004, 64, 1053-1057.

A simple and robust time-based on-line sequential injection system for trace mercury determination via cold vapour atomic absorption spectrometry (CVAAS), employing a new integrated gas-liquid separator (GLS), which in parallel operates as reactor, was developed. Sample and reductant are sequentially loaded into the GLS while an argon flow delivers the released mercury vapour through the atomic absorption cell. The proposed method is characterized by the ability of successfully managing variable sample volume up to 30 ml in order to achieve high sensitivity. For 20 ml sample volume, the sampling frequency is 25 h-1. The calibration curve is linear over the concentration range 0.05-5.0 κg l-1 of Hg(II), the detection limit is cL = 0.02 κg l-1, and the relative standard deviation is sr = 2.6% at 1.0 κg l-1 Hg(II) level. The performance of the proposed method was evaluated by analyzing certified reference material and applied to the analysis of natural waters and biological samples. Γ62 Direct determination of toxic metals in honey and sugars using inductively coupled

plasma atomic emission spectrometry. M.D. Ioannidou, G.A. Zachariadis, A.N. Anthemidis, J.A. Stratis Talanta, 2004, 65, 92-97.

A rapid method for the determination of Pb, Cd, Cu, Cr, Co, Ni, Mn and Zn in honey and sugars without prior digestion or ashing of the sample was developed, using inductively coupled plasma atomic emission spectrometry (ICP-AES). The critical instrumental parameters such as sample flow rate and radio frequency incident power were thoroughly optimized. The effect of matrix type and its concentration was also examined for glucose/fructose, sucrose and honey matrices. The sensitivity was investigated using calibration curves obtained in presence of the above matrices. The obtained recoveries for Cd, Cu, Cr, Co, Ni and Mn at the κg l-1 level were satisfactory and practically independent of the matrix used for the calibration standards. The recoveries of Pb and Zn were less sufficient. Various commercial samples of honey, sugar, glucose and fructose were analyzed with respect to their toxic metal content. The method can be applied for routine analysis, quality and environmental pollution control purposes at the κg l-1 level of concentration, after suitable dilution of the samples. Γ63 Comparison of a portable micro-X-ray fluoresence spectrometry with inductively

coupled plasma atomic emission spectrometry for the ancient ceramics analysis. D.N. Papadopoulou, G.A. Zachariadis, A.N. Anthemidis, N.C. Tsirliganis, J.A. Stratis Spectrochimica Acta Part B, 2004, 59, 1877-1884.

Two multielement instrumental methods of analysis, micro X-ray fluorescence spectrometry (micro-XRF) and inductively coupled plasma atomic emission spectrometry (ICP-AES) were applied for the analysis of 7th and 5th century B.C. ancient ceramic sherds in order to evaluate the above two methods and to assess the potential to use the current compact and portable micro-XRF instrument for the in situ analysis of ancient ceramics. The distinguishing factor of interest is that micro-XRF spectrometry offers the possibility of a nondestructive analysis, an aspect of primary importance in the compositional analysis of cultural objects. Micro-XRF measurements

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were performed firstly directly on the ceramic sherds with no special pretreatment apart from surface cleaning (micro-XRF on sherds) and secondly on pressed pellet disks which were prepared for each ceramic sherd (micro-XRF on pellet). For the ICP-AES determination of elements, test solutions were prepared by the application of a microwave-assisted decomposition procedure in closed high-pressure PFA vessels. Also, the standard reference material SARM 69 was used for the efficiency calibration of the micro-XRF instrument and was analysed by both methods. In order to verify the calibration, the standard reference materials NCS DC 73332 and SRM620 as well as the reference materials AWI-1 and PRI-1 were analysed by micro-XRF. Elemental concentrations determined by the three analytical procedures (ICP-AES, micro-XRF on sherds and micro-XRF on pellets) were statistically treated by correlation analysis and Student's t-test (at the 95% confidence level). Γ64 Determination of arsenic (III) and total inorganic arsenic in water samples using an

on-line sequential insertion system and hydride generation atomic absorption spectrometry. A.N. Anthemidis, G. A. Zachariadis, J.A. Stratis Analytica Chimica Acta, 2005, 547, 237-242.

A simple and robust on-line sequential insertion system coupled with hydride generation atomic absorption spectrometry (HG-AAS) was developed, for selective As(III) and total inorganic arsenic determination without pre-reduction step. The proposed manifold, which is employing an integrated reaction chamber/gas-liquid separator (RC-GLS), is characterized by the ability of the successful managing of variable sample volumes (up to 25 ml), in order to achieve high sensitivity. Arsine is able to be selectively generated either from inorganic As(III) or from total arsenic, using different concentrations of HCl and NaBH4 solutions. For 8 ml sample volume consumption, the sampling frequency is 40 h-1. The detection limit is cL = 0.1 and 0.06 κg l-1 for As(III) and total arsenic, respectively. The precision (relative standard deviation) at 2.0 κg l-1 (n = 10) level is sr = 2.9 and 3.1% for As(III) and total arsenic, respectively. The performance of the proposed method was evaluated by analyzing the certified reference material NIST CRM 1643d and spiked water samples with various concentration ratios of As(III) to As(V). The method was applied for arsenic speciation in natural waters samples This paper was placed 25th in the ANAL. CHIM. ACTA list of downloads. Γ65 Οn-line speciation of mercury and methylmercury by cold vapor atomic absorptiνn

spectrometry, using selective solid phase extraction. G. A. Zachariadis, A. N. Anthemidis, Δ. Daftsis, J.A. Stratis

Journal of Anaytical Atomic Spectrometry, 2005, 20, 63-65. A novel non-chromatographic on-line cold vapour atomic absorption spectrometric (CV-AAS) method for sequential mercury speciation at the ng l -1 level was developed based on the selective retention of inorganic mercury on polytetrafluoro ethylene (PTFE) using a dual manifold. A column packed with PTFE turnings was used for inorganic mercury separation from the sample solution via the efficient retention on the sorbent material of the pyrrolidine dithiocarbamate complex Hg(PDC)2. On the other hand, the PDC complex of methylmercury (CH3HgPDC) is barely adsorbed, thus facilitating its direct determination after reduction by NaBH4 and subsequent on-line thermal dissociation of the resulting hydride. Inorganic mercury in the presence of methylmercury species is determined in a parallel manifold due to the fact that the later one cannot be reduced by SnCl 2. The recovery of the proposed method was evaluated for drinking water, sea-water and urine samples. Γ66 Development and validation of routine analysis methods for the determination of

essential, non-essential and toxic minor and trace elements in cereals and cereal flour samples by inductively coupled plasma - atomic emission spectrometry.

Momen Awwad, G. A. Zachariadis, A. N. Anthemidis, J.A. Stratis Journal of AOAC 2005, 88, 1797-1810.

Various digestion procedures were carefully investigated and accurately evaluated with respect to their effect on the analysis of cereals and cereal flours. Multielement methods were selected and well developed for the determination of essential (Cr, Cu, Fe, Mg, Mn, and Zn), nonessential (Ag, Al, Ba, Bi, In, and Ga), and toxic (Cd and Pb) minor and trace elements by inductively coupled

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plasma-atomic emission spectrometry. Only Ag could be determined, either with aqueous standard or standard addition calibration methods, while the standard addition methods were more accurate for the determination of other elements. The recoveries were mostly within the range of 84.1-113% for the expected values of all analytes with respect to certified reference material NIST SRM 1586a (rice flour). The results proved that, for cereals and cereal flours, the use of H2O2 for wet digestion and HNO3 for dry ashing were not necessary. Linear regression analysis and Student's paired t-test were applied to evaluate the significant differences between different procedures and type of samples. Γ67 Optimized microwave-assisted decomposition method for multi-element analysis of

glass standard reference material and ancient glass specimens by inductively coupled plasma atomic absorption spectrometry. G.A. Zachariadis*, E. Dimitrakoudi, A.N. Anthemidis, J.A. Stratis Talanta 2006, 68, 1448-1456.

A novel microwave-assisted wet-acid decomposition method for the multi-element analysis of glass samples using inductively coupled plasma atomic emission spectrometry (ICP-AES) was developed and optimized. The SRM 621 standard reference glass material was used for this purpose, because it has similar composition with either archaeological glass specimens or common modern glasses. For the main constituents of SRM 621 (Ca, Na, Al, Fe, Mg, Ba and Ti), quality control data are given for all the examined procedures. The chemical and instrumental parameters of the method were thoroughly optimized. Thirteen acid mixtures of hydrochloric, nitric, and hydrofluoric acids in relation to two different microwave programs were examined in order to establish the most efficient protocol for the determination of metals in glass matrix. For both microwave programs, an intermediate step was employed with addition of H 3BO3 in order to compensate the effect of HF, which was used in all protocols. The suitability of the investigated protocols was evaluated for major (Ca, Na, Al), and minor (Fe, Mg, Ba, Ti, Mn, Cu, Sb, Co, Pb) glass constituents. The analytes were determined using multi-element matrix matched standard solutions. The analytical data matrix was processed chemometrically in order to evaluate the examined protocols in terms of their accuracy, precision and sensitivity, and eventually select the most efficient method for ancient glass. ICP-AES parameters such as spectral line, RF power and sample flow rate were optimized using the proposed protocol. Finally, the optimum method was successfully applied to the analysis of a number of ancient glass fragments. Γ68 Development and optimization of a portable-micro-XRF method for the in situ multi-

element analysis of ancient ceramics D.N. Papadopoulou, G.A. Zachariadis, A.Ν. Anthemidis, N.C. Tsirliganis, J.A. Stratis*

Talanta 2006, 68, 1692-1699. Non-destructive analysis of cultural objects by micro-XRF spectrometry is an advantageous multi-element technique that has rapidly developed during the past few years. Portable instruments contribute significantly to the in situ analysis of valuable cultural objects, which cannot be transported to the laboratory. Ancient ceramics are the most common archaeological findings and they carry a significant historical content. Their analysis often presents certain particularities due to surface irregularities and heterogeneity problems. In the present work, the analytical characteristics (beam spot size, geometry effect and detection limits) of a compact and portable micro-XRF instrument with a monocapillary lens are presented in details. The standard reference materials SARM 69, SRM 620, NCS DC 73332 and the reference materials AWI-1 and PRI-1 were analysed for the determination of the detection limits (DL's) and the evaluation of the accuracy of the micro-XRF. Emphasis is given on the critical parameters, which should be monitored during measurements and influence the final results in the analysis of ancient ceramics. A quantitative analysis of ancient ceramic samples from Abdera (North Greece) is also presented. Γ69 Investigation of four digestion prosedures for multi–element determination of toxic

and nutrient elements in legumes by inductively coupled plasma optical emission spectrometry A. Α. Momen, G. A. Zachariadis*, A. N. Anthemidis, J.A. Stratis

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Anal. Chim. Acta, 2006, 565, 81-88 A simple and reliable multi-element procedure for determination of essential (Cr, Cu, Fe, Mg, Mn, Zn) and toxic (Al, Cd, Pb) elements in legumes by inductively coupled plasma-optical emission spectrometry (ICP-OES) was developed. In this contribution, four different digestion procedures were thoroughly investigated and accurately evaluated with respect to their affect on the analysis of legumes. These included wet digestion with HNO3/H2SO4 and HNO3/H2SO4/H2O2, and dry ashing with Mg(NO3)2 and Mg(NO3)2/HNO3. Two calibrations (aqueous standard and standard addition) procedures were studied, and proved that standard addition was preferable for all analytes. ICP-OES operating parameters, such as radio-frequency (RF) incident power, sample uptake flow rate and nebulizer argon gas flow rate were optimized. The precision as repeatability, expressed as relative standard deviation (R.S.D.) for aqueous standard containing 250 κg l-1 of each analyte was in the range1.5-8.0%. The accuracy, expressed as relative error was generally varied in the range of 0.5-10% for all analytes, while the quantification limits were lower than 2.5 κg g-1. Although, acceptable results were obtained from all developed procedures, wet digestion method with HNO3/H2SO4/H2O2 is recommended for better recovery. The good agreement between measured and certified concentrations with respect to IAEA-331 and IAEA-359 (CRM's supplied by IAEA, International Atomic Energy Agency) indicates that the developed analytical method is well suited for determination of toxic and nutrient elements in legumes and possibly similar matrices. Γ70 Development of a rapid multi-element method of analysis of antitussive syrups by

inductively coupled plasma atomic emission spectrometry and direct sample introduction. G.A. Zachariadis*, D. Kapsimali, J. Pharm. Biomed. Anal. 2006, 41, 1212-1219

A new rapid method was developed and optimized for routine multi-element determination of traces of metals in antitussive syrups using direct introduction of diluted syrup into the nebulization system of inductively coupled plasma atomic emission spectrometer (ICP-AES). Using a Scott-type double-pass spray chamber combined with a cross-flow nebulizer, the optimum ICP conditions, like RF incident power, argon gas flow rate and nebulizer sample uptake flow rate were found. A critical objective of the study was to evaluate the matrix effect on the intensity and consequently on the sensitivity of the developed method. Thus, the maximum syrup concentration which could be introduced into the argon plasma, was estimated. The sensitivity variation was calculated as compared to the corresponding sensitivity obtained from aqueous solutions for each analyte. The performance characteristics of the proposed method were evaluated for quantitative and semi-quantitative determination and finally, the method was applied to the analysis of various commercial antitussives. Γ71 Use of fractional factorial design for optimization of digestion procedures followed

by multi-element determination of essential and non essential elements in nuts using ICP-OES technique. Α.Α. Momen, G. A. Zachariadis*, A. N. Anthemidis, J.A. Stratis Talanta, 2007, 71, 443-451

Two digestion procedures have been tested on nut samples for application in the determination of essential (Cr, Cu, Fe, Mg, Mn, Zn) and non-essential (Al, Ba, Cd, Pb) elements by inductively coupled plasma-optical emission spectrometry (ICP-OES). These included wet digestions with HNO3/H2SO4 and HNO3/H2SO4/H2O2. The later one is recommended for better analytes recoveries (relative error < 11%). Two calibrations (aqueous standard and standard addition) procedures were studied and proved that standard addition was preferable for all analytes. Experimental designs for seven factors (HNO3, H2SO4 and H2O2 volumes, digestion time, pre-digestion time, temperature of the hot plate and sample weight) were used for optimization of sample digestion procedures. For this purpose Plackett-Burman fractional factorial design, which involve eight experiments was adopted. The factors HNO3 and H2O2 volume, and the digestion time were found to be the most important parameters. The instrumental conditions were also optimized (using peanut matrix rather than aqueous standard solutions) considering radio-frequency (rf) incident power, nebulizer argon gas flow rate and sample uptake flow rate. The analytical performance, such as limits of detection (LOD < 0.74 κg g-1), precision of the overall

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procedures (relative standard deviation between 2.0 and 8.2%) and accuracy (relative errors between 0.4 and 11%) were assessed statistically to evaluate the developed analytical procedures. The good agreement between measured and certified values for all analytes (relative error <11%) with respect to IAEA-331 (spinach leaves) and IAEA-359 (cabbage) indicates that the developed analytical method is well suited for further studies on the fate of major elements in nuts and possibly similar matrices. Γ72 Analytical performance of ETAAS method for Cd, Co, Cr and Pb determination in

blood fraction samples. E.Daftsis, G.A. Zachariadis* Talanta, 2007, 71, 722-730

An electrothermal atomic absorption method (ETAAS) for direct determination of several toxic trace elements (Cd, Co, Cr, Pb) in human blood fractions was developed, because of increasing interest of toxic elements distribution in various blood constituents. Zeeman background correction and pyrolitically coated graphite tubes with L'vov platforms were used. Centrifugation was employed for the separation of blood fractions at different centrifugal conditions at 1200 ? g and 3000 ? g. The samples were acid-digested by HNO3 in closed tubes under high temperature and pressure before injection into graphite furnace. Two common modifiers were used and were compared for their effectiveness to the determination of each analyte at the examined blood fractions. The effect of modifier, matrix, calibration technique and peak characteristic (peak area and peak height) on the total variation of the method was examined by analysis of variance. The sensitivity and recovery (Cd 98-110%, Cr 93-109%, Co 95-106% and Pb 91-107%) of the developed method are presented for the various fractions. The overall precision (R.S.D.) using peak area (Cd 6.3-13.1%, Cr 8.2-13.9%, Co 7.4-8.5% and Pb 7.0-11.8%) and peak height measurements (Cd 1.1-9.3%, Cr 6.5-13.5%, Co 6.5-17.3% and Pb 6.9-14.8%) are also presented for pellet and supernatant solution. Standard addition technique was more accurate in terms of analyte recovery. Γ73 Development of a slurry introduction method for multi-element analysis of

antibiotics by inductively coupled plasma atomic emission spectrometry using various types of spray chamber and nebulizer configurations. G.A. Zachariadis*, C. E. Michos J. Pharm. Biomed. Anal. 2007, 43, 951-958.

A direct sample introduction inductively coupled plasma atomic emission spectrometric (ICP-AES) method, for multi-element analysis of powdered antibiotic drugs was developed using the slurry formation technique. The slurry of powdered sample is formed in dilute nitric acid solution in presence of Triton X-100 surfactant. Two different configurations of spray chamber and nebulizer were tested for direct aspiration of slurry into the plasma: (i) cyclonic spray chamber combined with babington-type nebulizer and (ii) scott-type double-pass spray chamber combined with cross-flow nebulizer. The latter configuration proved to be less tolerable to slurry aspiration. RF power generator, nebulizer argon gas flow rate, nebulizer sample uptake flow rate and slurry sample concentration were optimized. The sensitivity of the proposed method was compared to the corresponding sensitivity obtained from aqueous solutions for each analyte. The performance characteristics of the slurry aspiration method were evaluated against the complete acid-digestion method followed ICP-AES. Finally, the proposed method was applied to the analysis of commercial antibiotics. Γ74 Analytical performance of a multi-element ICP-AES method for Cd, Co, Cr, Cu, Mn, Ni

and Pb determination in blood fraction samples E.Daftsis, G.A. Zachariadis* Microchimica Acta, 2008, 160, 405-411.

An analytical method using inductively coupled plasma atomic emission spectrometry (ICP-AES) for rapid simultaneous determination of seven heavy metals (Cd, Co, Cr, Cu, Mn, Ni and Pb) in human blood fractions, like plasma, cells fraction and whole blood, is performed. The optimum wavelength was selected using as criterions the sensitivity, the linearity and recovery of aqueous standard solutions. The pretreatment of the sample, the centrifugal conditions, the necessity of digestion and the dilution of the digests were also studied. For plasma it was possible to avoid digestion of the

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sample, but for cells fractions and whole blood the digestion is necessary. The samples were acid-digested by HNO3 in closed Teflon tubes under high temperature and pressure conditions and were diluted before injection into ICP-AES. Also, optimization of the inductively coupled plasma conditions like nebulizer argon flow rate, sample flow rate and power of radio frequency was performed for each analyte. Finally, the effect of the type of the employed calibration technique on the total variation of the method was examined. Calibration using the standard addition technique was proved more accurate for the determination in terms of analyte recovery. The sensitivity and recovery (Cd 99%, Co 101%, Cr 100%, Cu 99%, Mn 101%, Ni 100% and Pb 97%) of the developed method are presented for all examined blood fractions. Γ75 Optimization and comparison of two digestion methods for multi-element analysis

of certified reference plant materials by ICP–AES. Application of Plackett-Burman and central composite designs.

Α.Α. Momen, G. A. Zachariadis*, A. N. Anthemidis, J.A. Stratis Microchimica Acta, 2008, 160, 397-403.

Two open-vessel wet digestion methods using mixtures of (i) HNO3 and H2SO4 (WD I), and (ii) HNO3, H 2SO4 and H2O2 (WD II) were developed and optimized for determination of a wide range of elements in plant reference materials by inductively coupled plasma atomic emission spectrometry. A Plackett-Burman fractional factorial experimental design with eight experiments for seven variables was used for the evaluation of the effects of several digestion variables at once. From these studies, certain variables showed up as significant, and they were further optimized by using a star-type central composite experimental design, which involved fourteen experiments. Instrumental variables such as radio-frequency incident power, nebulizer argon gas flow rate and sample uptake flow rate were also optimized. The analytical performance was assessed statistically. Nine elements in total can be simultaneously determined at the concentration levels usually found in plant materials. The agreement between measured and certified values with respect to NIST-SRM 1568a (rice flour), IAEA-331 (spinach leaves) and IAEA-359 (cabbage) proved that the developed methods are well suited for routine elemental analysis of plants or foods of plant origin Γ76 Arabino-Galactan Proteins from Pistacia lentiscus var. chia: isolation,

characterization and biological function F. Kottakis, F. Lamari, Ch. Matragkou, G. Zachariadis, N. Karamanos, and T. Choli-Papadopoulou. AminoAcids, 2008, 34, 413-420

Arabino-Galactan Proteins (AGPs) were isolated from Chios mastic gum (CMG) by using a buffer containing 0.1 M NaCl, 20 mM Tris–HCl at pH 7.5. Protein analytical methods, combined with specific procedures for carbohydrate characterization, indicated the presence of highly glycosylated protein backbone. In particular, staining by Yariv reagent of the electrophoretically separated molecules revealed the existence of arabinose and galactose and such a modification is characteristic for AGPs. After experiments involving extensive dialysis of the isolated extracts against water and atomic absorption, there was evidence of the existence of zinc ions that are probably covalently bound to the AGPs. By using anion-exchange chromatography, capillary electrophoresis, colorimetric methods and GC-MS, it was found that the extracts were separated into three major populations (A, B, and C), which were consistent with their respective negative charge content namely, uronic acid. The characterization of neutral sugars that was investigated with GC-MS showed the existence of arabinose and galactose in different amounts for each group. Experiments concerning the inhibition of growth of Helicobacter pylori in the presence of AGPs, as is shown for other CMG constituents, showed that the extracts of at least 1.4 g CMG affected the viability of the bacterium. There is no evidence as to whether the AGPs provoke abnormal morphologies of H. pylori, as is reported for the total CMG, or for O-glycans that possess terminal a1, 4-linked N-acetylglucosamine and are expressed in the human gastric mucosa; this has to be further investigated. Γ77 Evaluation and comparison of two combinations of pneumatic nebulizers and spray

chambers for direct slurry aspiration and multielement analysis of infant milk powders by axial-viewing inductively coupled plasma atomic emission spectrometry G.A. Zachariadis*, L.I. Valianou

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Applied Spectroscopy, 2008, 62, 716-720. Two different introduction systems were used for the determination of multielement content of infant milk powders by inductively coupled plasma optical emission spectrometry (ICP-AES). The first system consisted of cross-flow nebulizer and double-pass spray chamber while the second of V-groove type nebulizer and cyclonic spray chamber. Additionally an alternative and simple method is proposed and evaluated for both introductions devices. The aforementioned procedure includes only dilution of small aliquots of powdered milk and direct introduction of suspensions without involving time consuming mineralization stage. A method of sample mineralization by wet acid digestion was developed as well, for comparative purposes. Standard addition procedures were used to minimize possible matrix interferences. The elements that were determined are: Ag, Al, B, Ba, Bi, Ca, Cd, Co, Cr, Cu, Fe, Ga, In, Mg, Mn, Ni, Pb, Zn, As, and Se. Method validation was performed by measuring a SRM NIST 1549 non fat milk powder and according to its certificate, recoveries was oscillated between 93-114%. The precision of the method expressed as relative standard deviation was better than 5% for all analytes. After validation the proposed method has been applied to the multielemental analysis of five available commercial powdered infant formulas. The results processed by statistical methods, two-way Analysis of Variance (ANOVA) and Cluster analysis. Γ78 Use of slyrry suspension sample introduction technique in multielement analysis of

multivitamin preparations by inductively coupled plasma atomic emission spectrometry George A. Zachariadis*, Agathi F. Olympiou J. Pharm. Biomed. Anal. 2008, 47, 541-546.

A slurry suspension sampling technique has been developed and optimized for multi-elemental analysis of multivitamin and/or multimineral preparations using inductively coupled plasma – atomic emission spectrometry (ICP-AES). The following macro- and trace-elements Ca, Mg, Mn, Fe, Cr, Al, Ag, B, Ba, Bi, Cd, Co, Cu, Ga, In, Ni, Pb, Zn, As and Se were determined by the proposed method, and in addition a wet acid mineralization technique, was applied for comparative purposes. Samples were prepared as slurries in aqueous acidic media at a concentration of 5 % m/v. For the analyzed commercial preparations, the found element concentration is compared to the amount declared by producer. Obtained results were subjected to two – way analysis of variance (ANOVA) to ascertain the homogeneity across the analytical procedure and also to multiple linear regression analysis. Finally, the slurry ICP–AES technique was found to be applicable to routine quality control and contamination monitoring of multivitamins preparations. Γ79 Effect of sample matrix on sensitivity of mercury and methylmercury quantitation in

human urine, saliva and serum using GC-MS G. A. Zachariadis*, D. C. Kapsimali, J. Sep. Sci. 2008, 31, 3884 – 3893

Organomercury species like methyl-, dimethyl-, diethyl- and phenyl- mercury, are extremely important toxic species of mercury, due to their high toxicity for almost all living organisms, and especially to humans. These compounds are found in the environment either due to natural formation after alkylation processes of inorganic mercury species by specific bacteria, or because of extended use in the past as antibacterial and agricultural drugs. Although almost all of these compounds are banned in the many countries, still residues of such species are found in the environment, natural waters, foods and biological tissues. The most powerful method for speciation analysis of mercury is the hyphenation of capillary column gas chromatography with a mass spectrometric detector (GCMS). Due to their high volatility, careful optimisation of the chromatographic conditions is a critical point in order to achieve sufficient sensitivity and accuracy of the determination. Standards of all the above compounds were prepared and analysed in hexane and methanol solvents in splitless mode of injection. High resolution, non-polar polydimethylosiloxane capillary columns were applied for the gas chromatographic separation in a constant helium flow rate protocol. Electron ionisation mass detector equipped with a quadrupole mass analyser was also used for the separation of the species and identification. All organomercury species were detected and identified and the sensitivity for most of them was at the ng or pg κL-1 level.

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Γ80 Determination of butyl- and phenyltin compounds in human urine by HS-SPME after

derivatization with tetraethylborate and subsequent determination by capillary GC with microwave-induced plasma atomic emission and mass spectrometric detection G. A. Zachariadis*, E. Rosenberg, Talanta, 2009, 78, 570-576.

A head-space solid phase micro-extraction (HS-SPME) method was developed and optimized for gas chromatographic separation and determination of commonly found organotin compounds in cases of organotin contamination of human urine. Butyl- and phenyltin compounds were in-situ derivatized to ethylated derivatives by sodium tetraethylborate (NaBEt4) directly in urine matrix. Various parameters affecting the yield of the SPME procedure were examined, by using tetrabutyltin as internal standard. The method was optimized for direct use in the analysis of undiluted human urine samples and mono-, di- and trisubstituted butyl- and phenyl compounds could be determined after a 15 min head-space equilibration time at room temperature. The selectivity of the microwave-induced plasma atomic emission detector (MIP-AED) as an element specific detector in combination with the relatively selective sample preparation technique of HS-SPME allowed the interference-free detection of the organotins in all cases. A quadrupole mass spectrometer detector (MD) was used in parallel for confirmation of the identity of molecular structure of the eluted compounds. The performance characteristics of the developed method are given either for determination of mixtures of these compounds. Finally the proposed method was applied to the analysis of several human urine samples. Γ81 Speciation of organotin compounds in urine by GC-MIP-AED and GC-MS after ethylation and liquid-liquid extraction

G. A. Zachariadis*, E. Rosenberg, J. Chromatogr B, 2009, 877, 1140-1144.

A method for the determination of organotin compounds in urine samples based on liquid-liquid extraction (LLE) in hexane and gas chromatographic separation was developed and optimized. Seven organotin species, namely monobutyltin (MBT), dibutyltin (DBT), tributyltin (TBT), tetrabutyltin (TeBT), monophenyltin (MPhT), diphenyltin (DPhT) and triphenyltin (TPhT), were in-situ derivatized by sodium tetraethylborate (NaBEt4) to form ethylated less polar derivatives directly in the urine matrix. The critical parameters which have a significant effect on the yield of the successive liquid-liquid extraction procedure were examined, by using standard solutions of tetrabutyltin in hexane. The method was optimized for use in direct analysis of undiluted human urine samples and ways to overcome practical problems such as foam formation during extraction, due to various constituents of urine are discussed. After thorough optimization of the extraction procedure, all examined species could be determined after 3 min of simultaneous derivatization and extraction at room temperature and 5 min phase separation by centrifugation. Gas chromatography with either a microwave-induced plasma atomic emission detector (MIP-AED) as element specific detector was employed for quantitative measurements while a quadrupole mass spectrometric detector (MS) was used as molecular specific detector. The detection limits were between 0.42-0.67 µg L-1 (as Sn) for the quantitative LLE-GC-MIP-AED method and the precision between 4.2 - 11.7%, respectively. Γ82 Internal standardization with yttrium spectral lines using axial-viewing inductively

coupled plasma atomic emission spectrometry for plant certified reference materials analysis G. A. Zachariadis*, C. Sarafidou, Microchimica Acta, 2009, 166, 77-81.

Elements rarely found in biological samples, like yttrium, can be used as potential internal standards to improve the precision and some times the accuracy of atomic emission measurements obtained during inductively coupled plasma excitation of the analytes. The rate of improvement achieved is dependent on many factors like the spectral line of the analyte, the spectral line of yttrium, the type of plasma and the matrix of the injected sample. In this work the performance of yttrium as internal standard was investigated when aspirating digested samples or alternatively slurries of certified plant reference materials. In addition the stability of the yttrium

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signal used for analyte signal normalization was examined for three ionic emission lines of yttrium. The results showed that the use of yttrium was beneficial however for slurry sample introduction technique it was not equivalently efficient in terms of overall recovery. In this case, the employment of a cyclonic instead of a doublepass spray chamber in combination with yttrium internal standardization leads to quantitative recoveries of analytes ranging between 90-102 %. Γ83 Multi-element method for determination of trace elements in sunscreen by ICP-AES

G. A. Zachariadis*, E. Sahanidou, J. Pharm. Biomed. Anal. 2009, 50, 342-348.

An inductively coupled plasma atomic emission spectrometric (ICP-AES) method was developed for multi-element analysis of sunscreen creams and lotions. The objective was the simultaneous determination of Ti, because TiO2 is the only authorized inorganic UV filter in the European Union, and several minor, trace or toxic elements (Al, Zn. Mg, Fe, Mn, Cu, Cr, Pb and B) in the final products. Two alternative pretreatment procedures were examined: (i) total acid digestion in closed pressurized vessels prior to sample introduction into the plasma and (ii) direct introduction of sample in the form of emulsified slurry. The latter was proved inefficient for several types of creamy samples due to the high viscosity and insolubility of them. Several acid mixtures were examined for wet digestion because of the complex and fatty matrix of creams and lotions. Plasma parameters like nebulizer argon gas flow rate and radiofrequency incident power were optimized in order to improve the atomization. The recovery of the proposed acid-digestion method was evaluated using spiked samples. The calculated recoveries were 95.0 % for Ti, 98.2 % for Zn and 101.3 % for Fe, and the detection limits were 0.2 κg g-1 for Ti, 0.2 κg g-1 for Zn and 0.5 κg g-1 for Fe, respectively. Possible interference from the presence of Ti on the sensitivity of each analyte was examined. Finally the method was applied successfully to several commercial sun protection products and the results were compared to those obtained by atomic absorption spectrometry as reference method. Γ84 Headspace and direct immersion solid phase microextraction procedures for

selenite determination in urine, saliva and milk by gas chromatography mass spectrometry. D.C.Kapsimali, G.A.Zachariadis* J Chromatography B, 2009, 877, 3210-3214.

Two solid phase microextraction modes were investigated and compared for their performance on the determination of selenites in various biological liquids like human urine and saliva and various types of milk. Using sodium tetraethylborate (NaBEt4) as ethylating reagent, selenites are converted in situ to volatile diethylselenides (DESe) in aqueous medium. The derivative is collected in situ by solid phase microextraction (SPME) using a silica fiber coated with poly(dimethylsiloxane) (PDMS) either from the headspace (HS-SPME) or directly from the liquid phase (LP-SPME) and finally determined by capillary GC/MS. Under optimum conditions of SPME, the GC separation was also optimized. Between the two examined microextraction techniques, direct immersion of the PDMS fiber in the liquid phase was proved less satisfactory. In contrast, the headspace procedure appears to be more efficient. The quantification of selenites was achieved in SIM mode with good analytical performance. A non-fat milk powder certified reference material was analyzed to evaluate the accuracy of the method. The overall precision of the method was ranged between 6.2% and 9.7%. Detection limits achieved were 0.05 κg L-1 for human urine, 0.08 κg L-1 for saliva and 0.03–0.06 κg L-1 in various milk matrices. Γ85 On-line preconcentration and determination of nickel and zinc in natural water

samples by flow injection - flame atomic absorption spectrometry using PTFE-turnings for column packing Aristidis N. Anthemidis*, George A. Zachariadis, John A. Stratis. Intern. J. Environ. Analyt. Chem., 2010, 90, 127-136.

A rapid, sensitive and low cost, time-based flow injection atomic absorption spectrometric method was described for the determination of nickel and zinc upon on-line preconcentration on a micro-column packed with polytetrafluoroethylene-turnings material. The metals formed on-line complexes with ammonium pyrrolidine dithiocarbamate from acidified solutions (pH 2.0) in the flow manifold and retained in the column. The preconcentrated elements were subsequently eluted quantitatively with

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isobutylmethylketone and injected directly into the nebulizer for atomization and measurement. The system offered improved flexibility, low back-pressure and applicability. The enhancement factors were 170 and 65 for Ni(II) and Zn(II) respectively. The respective detection limits were 0.5 and 0.3 κg L-1, and the precision, expressed as relative standard deviation, were 2.8 % (at 5.0 κg L-1) and 3.2 % (at 2.0 κg L-1) respectively. The accuracy of the developed method was sufficient and evaluated by the analysis of certified reference material and spiked environmental water samples. Γ86 Comparison of tetraethylborate and tetraphenylborate for selenite determination in

human urine by gas chromatography mass spectrometry, after headspace solid phase microextraction

D.C.Kapsimali, G.A.Zachariadis* Talanta 2010, 80, 1311-1317.

Two different derivatizing reagents were tested for the development of a fast and sensitive method for determination of selenites (SeIV) in human urine. The reagents were sodium tetraethylborate (NaBEt4) and tetraphenylborate (NaBPh4), respectively, and the procedure is based on in situ derivatization of selenites in aqueous medium. Selenite ions are converted to diethylselenide (DESe) or diphenylselenide (DPhSe) and subsequently collected from the headspace by solid phase microextraction using a silica fiber coated with polydimethylsiloxane (HS-SPME). Finally they are quantitated by GC/MS in SIM mode. Ethylation over phenylation was proved preferable for the headspace extraction because of the higher volatility of the diethyl- derivative of selenites. The optimization of the HS-SPME conditions was performed both in aqueous and urinary solutions. Under the optimum conditions for HS-SPME, the gas chromatographic conditions were also optimised. Between the two alkylation reagents tetraethylborate was proved more efficient and the quantitation was satisfactory. Aqueous certified reference materials were analyzed to evaluate the accuracy of the method. The precision of the method was 4.2 % and the calculated detection limit was 0.05 κg L-1 for human urine.

Γ87 A new approach to indophenol blue method for determination of ammonium in geothermal waters with high mineral contents N. M. Tzollas, G. A. Zachariadis*, A. N. Anthemidis. J. A. Stratis Intern. J. Environ. Analyt. Chem., 2010, 90, 115-126.

Determination of ammonia-nitrogen, one of the decay products of nitrogenous organic compounds, is frequently needed to evaluate the drinking water safety and environmental pollution. Although methods based on the Berthelot reaction are more sensitive than the Nessler one, their sensitivities are not sufficient for the determination of ammonia in saline or geothermal waters due to their dependence of the pH and the buffering capacity problems. The pH shift in seawater analysis resulting ηο lower sensitivity and slower reaction rate has been demonstrated. In addition the precipitation of magnesium as hydroxide in strong alkaline conditions is another problem. On the other hand ion chromatography (IC) is particularly useful for the separation, identification, and quantification of ammonium ion at the κg/L level. The objective of work at first is the application and comparison of the standard methods for the determination of ammonia in the analysis of geothermal waters samples. The geothermal waters have elements in high concentrations as well as high salinity and in many cases the matrix is similar with the seawater. This fact makes the determination of ammonia with the mentioned methods to be problematic. The scope of this work is to study the obstructive factors and the ways to overcome these problems in the determination of ammonia in the geothermal waters. A comparison and evaluation of standard methods ion chromatography and UV-VIS spectrophotometric ones for ammonia determination was investigated and finally an optimisation of method was proposed in order to overcome the above problems. Γ88 Speciation of inorganic tin and tetramethyltin compounds by headspace solid phase

microextraction and gas chromatography mass spectrometry N. M. Tzollas, G.A.Zachariadis*, J Separation Science, 2010, 33, 1610-1616,

A headspace solid-phase microextraction (HS-SPME) method coupled to gas chromatography mass spectrometry (GC–MS) was developed in order to determine trace levels of tetramethyltin and inorganic tin after ethylation to tetraethyltin in various matrices. The derivatization of iSn and

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the extraction of both TeMT and iSn as TeET were performed in one step. Sodium tetraethylborate (NaBEt4) was used as derivatization agent and the volatile derivatives were absorbed on a poly(dimethysiloxane) (PDMS)-coated fused silica fiber. The conditions for the HS-SPME procedure were optimized in order to gain in repeatability and sensitivity. Several critical parameters of GC-MS were also studied. The detection of (TeMT) and (iSn) as (TeET) peaks was performed by the selected ion monitoring (SIM) mode. The precision of the proposed method is satisfactory providing relative standard deviations (RSD) values below 10% for both tin species and good linearity up to 10 κg/L. The developed method was successfully applied to the determination of tin species in several samples like canned fish, fish tissues, aquatic plants, canned mineral water and sea water. The proposed headspace SPME–GC–MS method was proved suitable to monitor the concentration levels of toxic tin compounds in environmental and biological samples. Γ89 Poly(etheretherketone)-turnings, a novel sorbent material for lead determination by

flow injection flame atomic absorption spectrometry and factorial design optimization. A.N. Anthemidis, I.S.I. Adam, G.A. Zachariadis Talanta, 2010,81, 996-1002.

A novel hydrophobic sorbent material for on-line column preconcentration and separation systems coupled with atomic spectrometry was developed. Poly(etheretherketone) (PEEK) in the form of turnings was used as packing material and evaluated for trace lead determination in environmental samples. Sample and ammonium diethyl-dithiophosphate (DDPA) reagent were mixed on-line and the Pb(II)–DDPA complex was retained effectively on PEEK-turnings. Isobutyl methyl ketone (IBMK) was adopted for efficient analyte complex elution and subsequently transportation into the nebulizer–burner system for atomization. The developed sorbent material has shown, excellent chemical and mechanical resistance, fast adsorption kinetics permitting the use of high sample flow rates without significant loss of retention efficiency. For 120 s sample preconcentration time the sampling frequency was 20 h−1, the enhancement factor was 110, the detection limit (3s) was cL = 0.32 κg L−1, and the relative standard deviation (RSD) was sR = 2.2%, at the 50.0 κg L−1 Pb(II) level. The accuracy of the developed method was evaluated by analyzing certified reference materials. Γ90 A review of recent developments in sample pretreatment, separation and

hyphenated atomic and mass spectrometric techniques for organoselenium speciation in biological liquids G.A. Zachariadis*, D.C. Kapsimali, E. Rosenberg Current Organic Chemistry, 2010, 14, 2282-2299.

Since organoselenium species like selenoaminoacids, selenopeptides, alkylselenides etc. and also the inorganic species selenite, Se (IV), and selenite, Se (VI), are known to be present in biological liquids and tissues, some of them playing an important role, there is a constant demand to develop or improve current analytical methodologies in order to enable species-specific detection and determination of these species in biological samples. The availability of various atomization techniques in atomic spectrometry and also of various ionization techniques in mass and tandem mass spectrometry have significantly increased the analytical armoury for organoselenium speciation. The literature in this field is abundant and fast growing, thus in this review only recent developments reported during the last decade and referring to the application of modern hyphenated techniques based on atomic or mass spectrometric detection are reviewed. The available information is classified according to the sample pretreatment procedures applied, the separation techniques and detectors used. Γ91 An overview of the use of yttrium for internal standardization in Inductively Coupled

Plasma – Atomic emission spectrometry. G. A. Zachariadis*, C. Vogiatzis Applied Spectroscopy Reviews, 2010, 45, 220-239.

Internal Standardization is a widely used method for compensating non-spectral interference in ICP spectrometry. Emphasis is given on the choice of the suitable element and its specific spectral line as an efficient internal standard. This choice is the result of multi-variable evaluations and

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analytical applications. In ICP-AES and other spectrometric techniques several spectral lines of the same or different elements have been evaluated as potential internal standards. Yttrium spectral lines fulfill certain prerequisites and they are favored by a large number of researchers. In our discussed review, we present several reports of ICP-AES techniques which employ yttrium as the internal standard. These reports are classified according to specific sample origin and aim of the research. Γ92 Microwave-assisted acid extraction methodology for trace elements determination in

mastic gum of Pistacia Lentiscus by Inductively Coupled Plasma Atomic Emission Spectrometry. G. A. Zachariadis*, E. Spanou Phytochemical Analysis, 2011, 22, 31-35.

Considering food safety, the accurate knowledge of the level of several trace elements is always necessary. This stands also for the natural products of plants and at least to those resins used for human consumption or therapeutic treatments, like the mastic gum of pistacia lentiscus. The rapid analysis of gum and resins matrices is a challenge because there are decomposition difficulties of such complicated matrices. The objective was to develop an efficient multielemental analytical method for the determination of trace elements and to compare different procedures for analyte extraction when microwave-assisted digestion is applied. The inductively coupled plasma atomic emission spectrometric (ICP-AES) technique was applied and the optimum ICP conditions like radiofrequency power, argon flow rate and nebulizer sample uptake flowrate were found. The microwave-assisted procedure was compared to that with conventional heating. Since mastic and resinous materials are difficult for dissolution and extraction of trace element, influential acid mixtures containing hydrofluoric acid proved to be capable for quantitative extraction of the analytes. The digestion of mastic resin or similar matrices is significantly facilitated by using microwave radiation instead of conventionally heating since the obtained recovery for several analytes is much higher. It was proved that the acid mixture of HCl-HNO3-HF was the most efficient to result in complete sample digestion and recovery of the analytes. The performance characteristics of the developed method were evaluated against certified reference material and the method was proved reliable and applicable to the analysis of mastic gum and possibly to similar resinous matrixes. Γ93 Analytical performance of a fast multi-element method for titanium and trace

elements determination in cosmetics and pharmaceuticals by ICP-AES G. A. Zachariadis*, E. Sahanidou, Central European J Chemistry, 2011, 9, 213-217.

A multi-element analytical method based on inductively coupled plasma atomic emission spectrometry (ICP-AES) was developed for trace elements in pharmaceutical tablets and cosmetics. Titanium was also included in the analytes since it is widely used in pharmaceuticals. Critical ICP conditions, like RF incident power, argon gas flow rate and nebulizer sample uptake flow rate were optimized. The most sensitive spectral line of each analyte was selected as optimum for further study. Detection limits in the low κg g−1 range were obtained. Prior to chemical analysis, the samples were decomposed by acid digestion, using various mixtures of HCl, HNO3 and HF. Yttrium was used as a suitable internal standard in order to correct for possible matrix effects. The method was applied to the analysis of six different pharmaceutical products (anti-biotic, anti-inflammatory, anti-hypertensive) in the form of tablets with film coating and also three cosmetic products like hair and face masks. Γ94 Effect of traffic pollution on seed quality characteristics of Pinus brutia

P. Ganatsa, M. Tsakaldimi, G. A. Zachariadis Environmental and Experimental Botany, 2011, 74, 157-161.

This study was undertaken to examine to what degree traffic air pollution affect the reproductive material (the seeds) of Pinus brutia. The study focused on the investigation of the seeds morphological characteristics and physiological attitudes as well as heavy metal concentrations of P. brutia trees grown close the ring-road of Thessaloniki (Greece‘s second biggest city), polluted by heavy traffic conditions, and how these characteristics are changed with the distance from the pollution source. A sampling procedure was established for seed collection from various distances

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from the pollution source. Seeds were properly prepared, measured for their morphological characteristics and heavy metal concentrations, as well as for their physiological behaviour (germination and viability). Analysis of the results showed that even though there were no significant differences in seed heavy metal concentrations, and only few in seed morphological characteristics, related to the distance from the traffic pollution source, there was a significant alteration of physiological behaviour for the seeds originated closed to the traffic. All the seed collected close to the ring-road (sample distances 0 and 30 m from the road) were unable to germinate, even those subjected to pre-treatments and repeated germination trials over longer duration, while those collected from distances over 100 m from the ring-road showed a common germination behaviour. Γ95 An evaluation of the use of Yttrium and beryllium as internal standards in

inductively coupled plasma emission spectrometry for untreated aqueous solutions in presence of high concentrations of organic solvents and matrices. G. A. Zachariadis*, C. Vogiatzis Journal of Analytical Atomic Spectrometry, 2011, 26, 2030 - 2038

Non-spectral interferences are known to affect the robustness and precision of Inductively Coupled Plasma –Emission Spectrometry (ICP-AES), especially in the case where the ICP-AES instrumentation includes conventional nebulization systems and the samples should not be pre-treated or are specially prepared as in-house working standards. Under these circumstances, yttrium ionic lines and a beryllium atomic line may be evaluated as internal standards (ISs). In this study, the working standards were prepared with the addition of several reagents that are known to affect the plasma‘s robustness in a set of series of multi-element standard solutions. In the applied methodology, conventional equipment (cross-flow pneumatic nebulizer and a double-pass, scott-type spray chamber) was used and the operating parameters of ICP-AES were adjusted to produce a robust plasma. A sum of 39 analytical lines of 19 elements (Ag, Al, B, Ba, Bi, Ca, Cd, Co, Cr, Cu, Fe, Ga, In, Mg, Mn, Ni, Pb, Tl θαη Zn) were studied. The studied organic substances were solvents like dioxane and formic acid and also glucose and sucrose in various concentration levels. The overall sensitivity changes and the accuracy of the method were used as quantitative criteria to evaluate the effect of the presence of the above mentioned substances. The results are useful for the analysis of many kinds of untreated samples, as well as in the analysis of eluents from separation procedures in hyphenated ICP-AES methodologies. Γ96 Determination of cations of group IA and IIA in potable water, pharmaceuticals and

energy drinks by ion chormatography. G. A. Zachariadis*, A. Lyratzi, J. A. Stratis Central European Journal Chemistry, 2011, 9, 941-947.

An efficient ion chromatographic (IC) method was developed for the simultaneous quantitative determination of Li+, Na+, NH4

+, K+, Cs+, Ca2+, Mg2+, Sr2+, Ba2+ and Be2+ in energy drinks,

pharmaceutical and drinking water samples by non-suppressed conductometric detection. The separation of ten cations including ammonium was achieved using a cation-exchange column and low conductivity mobile phase. The mobile phase consisted of tartaric acid, dipicolinic acid and boric acid. The separation of the cations is completed in less than 20 min, with a flow rate of 1.2 mL/min. The separation is not affected by the existence of cations Co2+, Cr3+, Cd2+, Cu2+, Bi2+, Ag3+, Fe3+ and Zn2+ in concentrations up to 20 mg/L. Using an injection volume of 20 κL the obtained detection limits (S/N=3) were 0.003 mg/L, 0.02 mg/L, 0.01 mg/L, 0.01 mg/L, 0.10 mg/L, 0.01 mg/L, 0.02 mg/L, 0.02 mg/L, 0.003 mg/L and 0.1 mg/L, for Li+, Na+, NH4

+, K+, Cs+, Ca2+, Mg2+,

Sr2+, Be2+ and Ba2+ respectively. The intra-day repeatability (RSD%, n=5) ranged from 1.1 % to 4.8%, and the inter-day (n=5) between 1.8 % and 5.4% respectively. The method was applied to the analysis of various bottled and tap water, pharmaceutical preparations and energy drinks from the market. Γ97 Hypersep-SCX micro-cartridge for on-line flow injection-inductively coupled plasma

atomic emission spectrometry determination of trace elements in biological and environmental samples Aristidis N. Anthemidis*, Georgia Giakisikli, George A. Zachariadis

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Analytical Methods 2011, 3, 2108-2114. This work covers an investigation into the potential of developing an automated on-line column preconcentration system using the readily available micro-cartridge HyperSep SCX for fast simultaneous determination of trace Ag(I), Cd(II), Co(II), Cr(III), Cu(II), Fe(III), Mn(II), Ni(II), Pb(II) and Zn(II) ions by inductively coupled plasma atomic emission spectrometry. The proposed method was based on the quantitative sorption of target elements onto the surface of the strong cation exchange resin at pH ~ 2.0, and complete elution with 2.0 mol L-1 HCl. All factors affecting the performance of the system such as sample acidity, loading and elution flow rate, preconcentration time as well as radio frequency (RF) incident power and nebulizer gas flow rate were examined in detail. The noticeable reduced consumption of chemicals along with automatic manipulations enabled the realization of a simplified and relatively clean procedure with low detection limits in the range of 0.05 and 0.23 κg L-1. Under the optimal conditions, the developed method provided a sampling frequency of 24 h-1 for all 10 studied elements. The accuracy of the developed method was evaluated by analyzing certified reference materials and spiked environmental natural water samples. Γ98 Feasibility and effectiveness of inhaled carboplatin in NSCLC patients.

P. Zarogoulidis, E. Eleftheriadou, I. Sapardanis, V. Zarogoulidou, H. Lithoxopoulou, T. Kontakiotis, N. Karamanos, G. Zachariadis, M. Mabroudi, A. Zisimopoulos, K. Zarogoulidis Investigational New Drugs, 2012, 30, 1628-1640.

Background Inhaled chemotherapy is under investigation as an alternative therapeutic modality for Non-Small Cell Lung Cancer. 60 NSCLC patients were randomized into 3 groups in this study. 20/60 patients (group A—control group) received I.V. chemotherapy (carboplatin AUC ≈ 5.5 D1); 20/60 (group B) received 2/3 of I.V. predicted carboplatin dose by I.V. infusion and the rest 1/3 as aerosol (jet nebulised D1); and 20/60 (group C) received all the predicted I.V. dose of carboplatin as aerosol in 3 equally divided fractions D1-3. In all patients I.V. docetaxel 100/m2 was as well administered (D1). Lung functional tests were performed in all groups before chemotherapy in the 3rd and 6th cycles. According to the obtained results, Group B had a statistically significant increase in survival compared to control group A [275 days (95% CI 249–300) vs. 211 (95% CI 185–236)]. In regard to lung functional tests, a statistically significant decline was observed only in FEV1 of group C in 6 months compared to the initial measurement. It was finally concluded that inhaled carboplatin could be given as an alternative root of pulmonary drug delivery in selected patients, but further randomized studies remain to prove whether the inhaled chemotherapy is an efficient and safe treatment modality.

Γ99 Speciation of Cr(III) and Cr(VI) using solid phase extraction and determination of total As by inductively coupled plasma atomic emission spectrometry. G. A. Zachariadis*, E. Trikas Intern J Environ. Analytical Chemistry 2012, 92, 385-391.

An inductively coupled plasma atomic emission spectrometric (ICP-AES) method was developed for speciation and simultaneous determination of Cr and As, since these two analytes are commonly determined in various water samples in order to assess their toxicity. The objective of this research was to study the speciation of Cr(III), Cr(VI) in presence of As(III) and/or As(V) using solid phase extraction (SPE) and ICP-AES. For these measurements, four spectral lines were used for each analyte with the purpose to select the most appropriate one for each element. Finally with the use for first time of a cation-exchange column filled with benzosulfonic acid and elution with HCl, the speciation in solutions which contained [Cr(III)+ Cr(VI)+ As(V)] and [Cr(III)+ Cr(VI)+ As(III)] was examined. It was proved that the separation of the two chromium species is almost quantitative and the simultaneous determination of chromium species and total arsenic analytes is possible, with very good performance characteristics. The estimated limits of detection for Cr(III), Cr(VI), As(III) and/or As(V) were 0.9 κg L-1, 1.1 κg L-1, 4.7 κg L-1 and 4.5 κg L-1

respectively, the calculated RSD were 3.8 %, 4.1 %, 5.2 % and 5.1 % correspondingly, and finally the accuracy of the methods was estimated using a certified aqueous reference material and found to be 5.6 % and 4.8 % for Cr(III) and Cr(VI) respectively. The method was applied to the routine analysis of various water samples.

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Γ100 Investigation of the HPLC-IT-TOF-MS Technique with Atmospheric Pressure Chemical Ionization for Speciation of Selenoaminoacids, Dimethyldiselenide, and Diphenyldiselenide D. Kapsimali, E. Rosenberg, G. A. Zachariadis* Analytical Letters, 2012, 45, 642-650.

The simultaneous determination of selenomethionine, selenoethionine, selenocystine, dimethyldiselenide and diphenyldiselenide was investigated for the first time by a hyphenated HPLC-IT-TOF-MS technique using atmospheric pressure chemical ionization (APCI). Alkylselenides and phenyldiselenides and also the most common selenoaminoacids were successfully determined after optimization of the instrumental and procedural parameters of the above system. Non-polar columns were studied in order to obtain baseline separation and speciation in less than 10 min. Selenium speciation was achieved successfully after optimization of the instrumental and procedural parameters of the above system. The APCI interface was studied at different zone temperatures and the most characteristic ions for each analyte were defined using both positive and negative polarity. The detection limits of the six selenium species were ranged between 13 – 59 κg/L and the relative standard deviation was between 4.2 - 9.3 % respectively. Γ101 Headspace solid phase microextraction for terpenes and volatile compounds

determination in mastic gum extracts, mastic oil and human urine by GC–MS G. A. Zachariadis*, A. Laggioli Analytical Letters, 2012, 45, 993-1003.

A reliable analytical method was developed, which is based on Headspace Solid Phase Microextraction (HS-SPME) and Gas Chromatography–Mass Spectrometry (GC-MS) for detection of volatile components of the gum and the essential oil of Pistacia lentiscus var. chia, commonly known as mastic gum and mastic oil respectively. The conditions of the HS-SPME were optimized and aqueous-ethanolic extracts of mastic gum and solutions of mastic oil in ethanol-water were analyzed by GC-MS. Almost 26 volatile components in mastic gum and 34 in mastic oil were identified in the gum and the oil respectively. The major constituents of the mastic gum and the mastic oil were α-pinene (63% and 76% respectively), β-myrcene (15% and 14%), β-pinene (4% and 4%), limonene (2.5% and 1.5%) and caryophyllene (5% and 1%). The quantitative determination of six of the above substances in multi-analyte standard solutions was achieved with good performance features. The repeatability (RSD%) was < 4.2% and the limits of detection were 1.2 κg/L for α-pinene, 0.09 κg/L for β-pinene, 0.7 κg/L for β-myrcene, 0.02 κg/L for camphene, 0.02 κg/L for p-cymene and 0.07 κg/L for α-terpineol. The HS-SPME/GC-MS procedure was successfully applied to samples of human urine samples after dietetic use of various mastic products such as mastic gum, mastic oil and a greek traditional higly viscous white mastic sweet. Traces of several constituents of mastic, such as α-pinene, β-myrcene, limonene, p-methyl anisole, terpinene, carveol, myrtenol, caryophyllene, α-caryophyllene, etc. were detected in the collected urine samples. Γ102 Far infrared study of local impurity modes of boron-doped PbTe.

P. M. Nikolic, K. M. Paraskevopoulos, G. Zachariadis, O. Valasiadis, T.T. Zorba, S.S. Vujatovic, N. Nikolic, O. S. Aleksic, T. Ivetic, O. Cvetkovic, V. Blagojevic, M.V. Nikolich, Journal of Material Science, 2012, 47, 2384-2389.

In this study, optical and electrical properties changes in lead telluride samples doped with a precisely measured atomic percentage of boron, in the range between 0.01 B and 2 at.% B were measured. The study focused mostly on samples with boron concentrations less than 0.5 at.% B because it was expected that boron atoms for single crystal samples just fill lead vacancies. PbTe single crystals, doped with B, were grown using the Bridgman method. Far infrared spectra were measured in the temperature range between 10 K and room temperature. The experimental spectra were numerically analyzed, and optical parameters were calculated. Local impurity modes of boron were observed at about 150 and 240 cm-1. For all samples, after FIR measurements, the content of boron was measured using inductively coupled plasma atomic emission spectrometry. Optical mobility of free carriers was calculated and it was the highest for the sample with only 0.014 at.% of boron in PbTe. A negative photoconductivity effect at 130 K for PbTe+B was also observed.

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Γ103 Determination of organolead and organotin compounds in urine and aqueous

samples by GC-MIP-AED and GC-MS after ethylation and liquid-liquid extraction G. A. Zachariadis*, E. Rosenberg, J Sep Science, 2012, 35, 1132-1137.

This work describes the development of a fast method for speciation of triethyl-lead (TEL), and tributyl-tin (TBT) species in urine samples after in situ derivatization by tetraethyl- or tetrapropyl-borate reagents. The alkylation reaction is done in the aqueous and urine medium and the less polar derivatives are extracted in hexane by liquid-liquid extraction (LLE). The species were extracted and the extract was efficiently collected from the aqueous phase after centrifugation. Finally the organometallic species are separated by gas chromatography and determined from the emission signals of elemental lead and tin. Atomic lead and tin are formed from the organolead and organotin compounds during atomization of the column eluate in a microwave induced helium plasma source (MIP). The simultaneous measurement of lead at 405.780 nm and Sn at 303.419 nm was achieved by an atomic emission detector (AED). The extraction step and the chromatographic separation were optimized using standard solutions in hexane. Finally the analytes were determined with satisfactory precision (RSD < 5%) and detection limits of 0.05 κg Pb /L (TEL) and 0.48 κg Sn/L (TBT) respectively, when 10 mL of urine is extracted with 1 mL of hexane and 1 κL of extract is injected. Γ104 Storage stability studies for tributyltin determination in human urine samples using

headspace solid phase microextraction and gas chromatography and mass spectrometry. N. M. Tzollas, M. Nikolaou, E. Rosenberg, G. A. Zachariadis* Biomedical Chromatography, 2012, DOI 10.1002/bmc.2791

A headspace solid-phase micro-extraction (HS-SPME) method was employed in order to study the effect of storage conditions of human urine samples spiked with tributyltin (TBT) using gas chromatography and mass spectrometry. To render the analyte more volatile, the derivatization (ethylation) was made in situ by sodium tetraethylborate (NaBEt4), which was added directly to dilute unpreserved urine samples and in buffers of similar acidity. The stability of TBT in human urine matrix was compared with the stability of TBT in buffer solutions of similar pH value. Critical parameters of storage conditions such as temperature and time, which affect the stability of TBT in this kind of matrix, were examined extensively. The tests showed that the stability of TBT remains practically satisfactory for a maximum of 2 days of storage either at +4 or 20 oC. Greater variations were observed in the concentration of TBT in human urine samples at +4 oC and lower ones at - 20 oC over a month‘s storage. The freeze–thaw cycles have negative effect on the stability and should be kept to a minimum. The results from spiked urine samples are also discussed in comparison to those acquired from buffer solutions of equal TBT concentration. Γ105 Speciation study of trialkyl- and triphenyl tin by liquid chromatography using Ion

Trap TOF tandem MS and atmospheric pressure chemical ionization. N. M. Tzollas, E. Rosenberg, G. A. Zachariadis* Current Analytical Chemistry 2012, In press.

In this work, the capability of a hybrid tandem technique of LCMS-IT-TOF was examined for speciation of tripropyltin (TPrT), tributyltin (TBT), tripentyltin (TPeT) and triphenyltin (TPhT) and a relatively fast and accurate method was developed for their determination in aqueous solutions. In this context, several chromatographic parameters were studied such as chromatographic column, mobile phase, flow rate, gradient program etc. in order to optimise the separation of the above species. Optimum separation was achieved using reverse phase C18 bonded-silica column. The mobile phase consisted of water and methanol and acetic acid at a total flow rate of 0.4 mL min–1

and the separation was completed in less than 8 min. Determination of the analytes was performed based on the signal of characteristic ions for each particular tri-organotin compound. The detection limits of the set of the above four tri-organotin species were ranged between 13 – 45 pg as absolute mass of Sn injected into the column and the relative standard deviation was ranged between 4.3 – 9.9 %. Finally, the performance characteristics of the method were compared to those reported in literature using similar tandem LC-MS techniques.

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Γ106 Editorial. Metal Speciation and metallomics.

G. A. Zachariadis* Current Analytical Chemistry 2013, Vol. 9, In press.

It is my Editorial preface text as Guest Editor in the Special issue of Current Analytical Chemistry journal, devoted to Metal Speciation and Metallomics. The metal-containing biomolecules like metalloproteins, metalloenzymes, etc., define the so-called metallome in the metallomics field, as it happens for genomes in genomics and proteomes in proteomics. It was Haragushi (2002) who proposed the term ‗‗metallomics‘‘ to describe the new scientific field with the purpose to integrate all research fields related to biometals science. Besides the apparent purpose to describe presence and distribution of metals and metal species in various biological systems, cells, organs, etc., research on metallomics is considered of great importance also to elucidate various complicated biological mechanisms. However, since the specific form of metal is critical for its behavior in the metallome, the metal speciation becomes a necessity, from the analytical point of view. It is reasonable to say that any analytical approach related to metallomics definitely relates to metal speciation also, although the reverse is not necessary. In this context, modern speciation analysis methods based on element- and species- specific hyphenated analytical techniques allowed the ultimate detection and identification of various metal species in any kind of biological matrix. This was the driving force to devote the present special issue to both the above described fields. Therefore one of the aims of this mini hot topic issue of Current Analytical Chemistry was to highlight the current trends in metal speciation analysis and also in metallomics.

Γ107. Use of Dohelert-type designs in the optimization of a hybrid Electrospray Ionization Ion Trap TOF-MS for glutathione determination G. A. Zachariadis*, E. Rosenberg

Rapid Communication in Mass Spectrometry, In Press. The capabilities of Doehlert-type experimental designs to optimize the performance of a hybrid mass spectrometer were investigated in this paper for the first time. In particular, a tandem technique based on sequential ion management by an ion trap followed by a time-of-flight mass analyzer (IT-TOF-MS) was introduced commercially in recent years and investigated for glutathione determination. The optimization process was completed in three successive steps with groups of variables avoiding any univariable approach. Glutathione (GSH, reduced) was selected as a very interesting analyte since it is considered to be one of the most abundant tripeptides in human organism and its action against xenobiotics and oxidative radicals is well known. Glutathione was injected in a stream of a typical mobile phase used in liquid chromatography and analyzed after electrospray ionization (ESI) in tandem MS. The three main steps of the LC-MS system, namely the LC mobile phase, the ESI interface and the MS analyzer were independently optimized in terms of maximum sensitivity. In this context quadratic models were found and their prediction power was evaluated. Finally, calibration study was performed at default and optimum conditions in order to quantitatively estimate the sensitivity enhancement of the employed technique for this analyte. The results highlighted the possibilities offered by the employment of multi-factorial optimization.

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PART ΙΙΙ PAPERS IN INTERNATIONAL PEER REVIEWED JOURNALS

This part is in two independent volumes and includes all the published papers in peer reviewed journals with code numbers Γ1, Γ2, …. Note that the CD includes all these papers in electronic form as PDFs with the same Part III directory name..

PART ΙV CITATIONS IN MY PAPERS

This part is an independent volume and includes all the citations without self-citations in my published papers in peer reviewed journals. In addition the CD includes a file with the list of all citations in electronic form, with the same Part IV directory name.

PART V ANOUNCEMENTS IN CONFERENCES

This part is an independent volume and includes all the announcements in international and national conference. Accordingly, the CD includes all these presentations in electronic form as abstracts or works, with the same Part V directory name.

PART VΙ MY DOCTORAL THESIS BOOK

In this part a printed copy of my Ph. D. thesis is submitted. An electronic copy of the scanned pages of the thesis book is included in the CD, with the same Part VI directory name.

PART VΙΙ INTERNATIONAL BOOK FOR ICP-AES TECHNIQUE

In this part the International monography under the title ―INDUCTIVELY COUPLED PLASMA ATOMIC EMISSION SPECTROMETRY: A MODEL MULTIELEMENT TECHNIQUE FOR MODERN LABORATORY‖ is included and a copy of the book is submitted. An electronic copy of the book is included in the CD, with the same Part VII directory name.

PART VΙIΙ COPIES OF MY TUTORIAL BOOKS

The tutorial books are included as printed copies of almost all of them. In addition the CD includes scanned first pages of those books, with the same Part VIII directory name.

PART IX FORMAL DOCUMENTS

In this part, scanned formal documents are included confirming my scientific, educational, administrative and other activities, with the same Part IX directory name.