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THE ASIAN CITRUS PSYLLID Asian citrus Psyllid sequenced Genome, Transcriptome, Metagenome Hunter, W, Shatters, R, D. Hall, US. Horticultural Research Lab, Ft. Pierce, FL. USDA,ARS, USHRL, Subtropical Insects Res. Unit, Funded Project. Invasion of psyllid In Florida, 1998. Detection of Huanglongbing in citrus trees 2005. First Psyllid EST’s, Expressed sequence tags produced from psyllids. Adults, Nymphs, Midgut, Testes, USDA, ARS, Hunter, et al., 2005 , 2006, 2008. Milestones in Psyllid Research GENOME Gene expression studies- Metagenome, Heat shocks, Insecticide resistance, Pathology, Marutani-Hert et al., 2009. Hunter et al, 2009. RNA interference, demonstrated in psyllids. Hunter et al, 2009. Application of Molecular markers reveal biotypes Boykin et al., 2010 Psyllid RNAi research expands. Shatters et al, 2010,2011 Hunter et al, 2010.-2011. De Leon, et al, 2010,. International Psyllid Genome Consortium established- 2008 Hunter, W., & Bextine, B. Gene expression studies, Cell Culture, microsatellites Boykin et al, 2007, 2008 Hert, et al., 2008 Hunter et al, 2008 A Model for Development Insights into Gene Regulation The Genome Beyond the Genome Milestones in Psyllid Research 1998-2005 2007-2008 2009 2010 2011 2005-2006 The International Psyllid Genome Consortium: http://www.uttyler.edu/biology/faculty/bextine/psyllid_consortium/index.php http://www.ncbi.nlm.nih.gov/genomeprj?Db=genomeprj&cmd=ShowDetailView&TermToSearch=29473 Arthropod Genomes: http://arthropodgenomes.org/wiki/Diaphorina_citri Halbert 2001 Halbert & Manjunath 2004 Author: Wayne B. Hunter, USDA, ARS (Diptera) (Lepidoptera) (Coleoptera) (Hymenoptera) (Phthiraptera) (Hemiptera) Fruit Flies Mosquito Silk Worm Flour Beetle Honey bee Parasitoid Wasps Louse Blood-feeding bug Pea Aphid Asian Citrus Psyllid 400 0 Divergence Time Millions of Yrs Holometabola Hemimetabola

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THE ASIAN CITRUS PSYLLID

Asian citrus

Psyllid sequencedGenome, Transcriptome,

Metagenome

Hunter, W, Shatters, R,

D. Hall, US. Horticultural

Research Lab, Ft. Pierce,

FL.

USDA,ARS, USHRL,

Subtropical Insects Res.

Unit, Funded Project.

Invasion of psyllid

In Florida, 1998.

Detection of

Huanglongbing

in citrus trees 2005.

First Psyllid EST’s,

Expressed sequence

tags produced from

psyllids.

Adults, Nymphs, Midgut,

Testes,

USDA, ARS,

Hunter, et al., 2005 ,

2006, 2008.

Milestones in Psyllid Research

GENOME

Gene expression

studies-Metagenome, Heat shocks,

Insecticide resistance,

Pathology,

Marutani-Hert et al., 2009.

Hunter et al, 2009.

RNA interference,

demonstrated in

psyllids.

Hunter et al, 2009.

Application of

Molecular markers

reveal biotypes

Boykin et al., 2010

Psyllid RNAi

research expands.

Shatters et al, 2010,2011

Hunter et al, 2010.-2011.

De Leon, et al, 2010,.

International Psyllid

Genome Consortium

established- 2008

Hunter, W., & Bextine, B.

Gene expression

studies, Cell Culture,

microsatellites

Boykin et al, 2007, 2008

Hert, et al., 2008

Hunter et al, 2008

A Model for

Development

Insights into

Gene Regulation

The Genome

Beyond the

Genome

Milestones in

Psyllid Research

1998-2005 2007-2008 2009 2010 20112005-2006

The International Psyllid Genome Consortium:

http://www.uttyler.edu/biology/faculty/bextine/psyllid_consortium/index.php

http://www.ncbi.nlm.nih.gov/genomeprj?Db=genomeprj&cmd=ShowDetailView&TermToSearch=29473

Arthropod Genomes: http://arthropodgenomes.org/wiki/Diaphorina_citri

Halbert 2001

Halbert & Manjunath

2004

Author: Wayne B. Hunter, USDA, ARS

(Diptera)

(Lepidoptera)

(Coleoptera)

(Hymenoptera)

(Phthiraptera)

(Hemiptera)

Fruit Flies

Mosquito

Silk Worm

Flour Beetle

Honey bee

Parasitoid Wasps

Louse

Blood-feeding bug

Pea Aphid

Asian Citrus Psyllid

400 0Divergence Time Millions of Yrs

Holo

meta

bola

Hem

imeta

bola