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Gene Expression *Protein coding gene *Gene expression Genes control inherited variation via: DNA, RNA and protein Phenotype *Gene DNA Phenotype RNA Protein ReplicationTranscriptionTranslation

Gene Expression *Protein coding gene *Gene expression Genes control inherited variation via: DNA, RNA and protein Phenotype *Gene DNAPhenotypeRNAProtein

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Page 1: Gene Expression *Protein coding gene *Gene expression Genes control inherited variation via: DNA, RNA and protein Phenotype *Gene DNAPhenotypeRNAProtein

Gene Expression

*Protein coding gene

*Gene expression

Genes control inherited variation via:

DNA, RNA and protein

Phenotype

*Gene

DNA PhenotypeRNA ProteinReplication TranscriptionTranslation

Page 2: Gene Expression *Protein coding gene *Gene expression Genes control inherited variation via: DNA, RNA and protein Phenotype *Gene DNAPhenotypeRNAProtein

Brief overview of transcription:

Page 3: Gene Expression *Protein coding gene *Gene expression Genes control inherited variation via: DNA, RNA and protein Phenotype *Gene DNAPhenotypeRNAProtein

Transcription*RNA polymerase (from E. coli )

2 ’

CoreSigma factor

Holoenzyme

Page 4: Gene Expression *Protein coding gene *Gene expression Genes control inherited variation via: DNA, RNA and protein Phenotype *Gene DNAPhenotypeRNAProtein

Transcription

Functions of a prokaryotic promoter:

1. ON/OFF Switch

2. Speed Switch

3. Alignment

Page 5: Gene Expression *Protein coding gene *Gene expression Genes control inherited variation via: DNA, RNA and protein Phenotype *Gene DNAPhenotypeRNAProtein

Binding of RNA Polymerase to Promoters

How tightly does core enzyme vs. holoenzyme bind DNA?

Page 6: Gene Expression *Protein coding gene *Gene expression Genes control inherited variation via: DNA, RNA and protein Phenotype *Gene DNAPhenotypeRNAProtein

Transcription Initiation

Page 7: Gene Expression *Protein coding gene *Gene expression Genes control inherited variation via: DNA, RNA and protein Phenotype *Gene DNAPhenotypeRNAProtein

Transcriptional Elongation:

Page 8: Gene Expression *Protein coding gene *Gene expression Genes control inherited variation via: DNA, RNA and protein Phenotype *Gene DNAPhenotypeRNAProtein

Termination of TranscriptionInverted Repeats and Hairpins

• The repeat at right is symmetrical around its center shown with a dot

• A transcript of this sequence is self-complementary– Bases can pair up

to form a hairpin as seen in the lower panel

Page 9: Gene Expression *Protein coding gene *Gene expression Genes control inherited variation via: DNA, RNA and protein Phenotype *Gene DNAPhenotypeRNAProtein

Termination of Transcription

*Rho-independent termination

Page 10: Gene Expression *Protein coding gene *Gene expression Genes control inherited variation via: DNA, RNA and protein Phenotype *Gene DNAPhenotypeRNAProtein

factor

Termination of Transcription*Rho-dependent termination